id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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34,995 | RNA extraction with spin columns from yeast cells grown on 12-column deep well plates | 1 | dx.doi.org/10.17504/protocols.io.beetjben | https://www.protocols.io/view/rna-extraction-with-spin-columns-from-yeast-cells-beetjben | Jamie Auxillos, Rosey Bayne, Edward Wallace | TITLE: RNA extraction with spin columns from yeast cells grown on 12-column deep well plates
AUTHORS: Jamie Auxillos, Rosey Bayne, Edward Wallace
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is for yeast cell growth, lysis, and RNA extraction using silica spin columns. We use ... | ["[Harvesting and lysis of cells]\nTake a screw cap tube (Sarstedt cat #12705493) and use a PCR tube to measure zirconia beads (BioSpec #11079105z) (note - fill the entire PCR tube with beads) . Add the zirconia beads into the screw cap tubes. Label each tube.", "[Harvesting and lysis of cells]\nResuspend the pellet in... |
66,324 | Via Keto Apple Gummies Canada Reviews: Keto BHB ACV Formula in Canada UK, USA | 3 | dx.doi.org/10.17504/protocols.io.bp2l616brvqe/v1 | https://www.protocols.io/view/via-keto-apple-gummies-canada-reviews-keto-bhb-acv-cczusx6w | ViaKeto Apple Gummies | TITLE: Via Keto Apple Gummies Canada Reviews: Keto BHB ACV Formula in Canada UK, USA
AUTHORS: ViaKeto Apple Gummies
[DESCRIPTION]
Via Keto Apple Gummies Canada Reviews: Keto BHB ACV Formula in Canada UK, USA
Visit the Official Website:- https://rvmac.net/viaketo-apple-gummies-canada/
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.jbncime | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.ix7cfrn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Suprarenal region of the abdominal aorta is the portion from the last intercostal artery to the right renal artery.In the Angiotensin II infused mouse model, abdominal aortic aneurysms are located in this region. Maximal outer width of the suprarenal aorta is a major paramete... | [] |
30,948 | SPARC - Setting up the BEADS for the Millipore Metabolic Rat Milliplex Assay | 1 | dx.doi.org/10.17504/protocols.io.bp2l6n9ergqe/v1 | https://www.protocols.io/view/sparc-setting-up-the-beads-for-the-millipore-metab-bagcibsw | Doris Kemler, J Paul Robinson | TITLE: SPARC - Setting up the BEADS for the Millipore Metabolic Rat Milliplex Assay
AUTHORS: Doris Kemler, J Paul Robinson
[DESCRIPTION]
The beads are made for two half plates and are good for 1 month.
[BEFORE_START]
The following are required:
Teal clip tips
5x16 Eppendorf rack
Multi-vortex tube holder
Vortex
Large... | ["Before making the beads, the filter plate must be pre-wet for at least 10 min.", "Add 75 µL to each well using the teal (15-1250µl) Eclip tip using only 6 pipette tips (see below for diagram), using settings Preset>stepper>pre-step, 75ul, 12x, 3, 4 (will pull up 900µl).\n\nUse only 6 tips (pipette twice in the same r... |
95,923 | LENTIVIRAL TRANSDUCTION OF HUMAN PLURIPOTENT STEM CELLS | 0 | null | https://www.protocols.io/view/lentiviral-transduction-of-human-pluripotent-stem-c9wtz7en | Renuka Ravi Gupta, Nona Farbehi, hendersa, Vikram Khurana, Gist Croft, Lorenz Studer, Joseph Powell | TITLE: LENTIVIRAL TRANSDUCTION OF HUMAN PLURIPOTENT STEM CELLS
AUTHORS: Renuka Ravi Gupta, Nona Farbehi, hendersa, Vikram Khurana, Gist Croft, Lorenz Studer, Joseph Powell
[DESCRIPTION]
We have developed a protocol for lentiviral transduction of human pluripotent stem cells (hPSCs), including induced pluripotent stem ... | ["[Day 0: Coating wells with VTN-N and seeding hPSCs] Add 60 ul of VTN-N to 6 ml of DPBS.", "[Day 0: Coating wells with VTN-N and seeding hPSCs] Coat 100 ul per well in a 48 - well plate.", "[Day 0: Coating wells with VTN-N and seeding hPSCs] Incubate the plate at room temperature for an hour and the plate is ready to ... |
43,724 | Introduction to Primer Design (Draft) | 3 | null | https://www.protocols.io/view/introduction-to-primer-design-draft-bnxkmfkw | TITLE: Introduction to Primer Design (Draft)
AUTHORS:
[STEPS] | [] | |
59,525 | Preparing multiplexed 16S/18S/ITS amplicon SMRTbell libraries with the Express TPK2.0 for the PacBio Sequel2 | 4 | dx.doi.org/10.17504/protocols.io.j8nlkk89wl5r/v1 | https://www.protocols.io/view/preparing-multiplexed-16s-18s-its-amplicon-smrtbel-b6ddra26 | André M Comeau, Gina V Filloramo | TITLE: Preparing multiplexed 16S/18S/ITS amplicon SMRTbell libraries with the Express TPK2.0 for the PacBio Sequel2
AUTHORS: André M Comeau, Gina V Filloramo
[DESCRIPTION]
The preparation of amplicon libraries using the PacBio Express Template Kit 2.0 (TPK2.0) with fusion primers at the IMR.
Based upon PacBio protoco... | ["[Barcoded PCRs] Prepare PCRs and verify by Coastal Genomics gels as in the IMR protocol Preparing multiplexed 16S/18S/ITS amplicons for the Illumina MiSeq, except only using Plates 1+2 (F1R1 + F1R2) of the PacBio working primers from the IMR protocol Preparing Combined Indexed Primer Plates (IDT Standard) for the Pac... |
38,168 | Protocol for reading and understanding a paper | 3 | null | https://www.protocols.io/view/protocol-for-reading-and-understanding-a-paper-bhhyj37w | Arindam Basu | TITLE: Protocol for reading and understanding a paper
AUTHORS: Arindam Basu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol for generating zettels from texts and books and articles. It includes techniques discussed by other authors. </div></div>
[STEPS] | [] |
97,940 | Quantification of tissue creatine content using capillary electrophoresis | 4 | dx.doi.org/10.17504/protocols.io.4r3l22j23l1y/v2 | https://www.protocols.io/view/quantification-of-tissue-creatine-content-using-ca-dbvu2n6w | Jane Choi, Angelo Bautista, Jessica Terrill, J. Jane Pillow, Peter G Arthur | TITLE: Quantification of tissue creatine content using capillary electrophoresis
AUTHORS: Jane Choi, Angelo Bautista, Jessica Terrill, J. Jane Pillow, Peter G Arthur
[DESCRIPTION]
The current protocol describes an alternative method for creatine quantification in biological tissue samples using capillary electrophores... | ["[Preparation of buffers] Prepare the stock phosphate buffer (PB) 200 millimolar (mM) pH 6", "[Preparation of buffers] pH the solution to 6.0 with concentrated NaOH", "[Preparation of buffers] top up the volume to 50 mL with ddH2O (ideally using a volumetric flask)", "[Preparation of buffers] filter sterilise the solu... |
78,438 | Immunostaining of iPSC-derived neurons | 1 | dx.doi.org/10.17504/protocols.io.e6nvwj4kdlmk/v1 | https://www.protocols.io/view/immunostaining-of-ipsc-derived-neurons-cquevwte | Dan Dou, Erika L.F. Holzbaur | TITLE: Immunostaining of iPSC-derived neurons
AUTHORS: Dan Dou, Erika L.F. Holzbaur
[DESCRIPTION]
Here, we fix, permeabilize, and stain human iPSC-derived neurons for the purpose of observing and quantifying somal and axonal abundance of proteins of interest. For preceding culture of neurons, see "Protocol: Culture an... | ["Replace culture media in dish with 1 mL room temperature BrainPhys culture media (Stemcell Technologies)", "Add 1 mL Bouin's solution (Sigma). Incubate at room temperature for 30 minutes.", "Wash at least 5 times with PBS. Yellow color should be absent.", "Permeabilize in ice-cold Optima methanol (Thermo Fisher) at -... |
79,948 | Total Starch Enzymatic Digestion | 1 | null | https://www.protocols.io/view/total-starch-enzymatic-digestion-csbkwakw | Lynn Doran, Amanda P. De Souza | TITLE: Total Starch Enzymatic Digestion
AUTHORS: Lynn Doran, Amanda P. De Souza
[DESCRIPTION]
Enzymatic digestion of total soluble starch to glucose in plant tissue extracts for preparation for quantification via the GOD-POD Method (NZYtech).
[BEFORE_START]
Extract and dry total starch pellet from plant tissue per... | ["Prepare fresh daily 120 U/mL α-amylase in MOPS buffer. 1 mL per sample will be needed. Initial concentration of α-amylase is 3000 U/mL. Use C1V1 = C2V2 to calculate the volume of α-amylase and MOPS buffer to use.", "Prepare fresh daily 30 U/mL amyloglucosidase in acetate buffer. 1 mL per sample will be needed. Initi... |
19,253 | Pseudorabies Virus (PRV) injection into interscapular brown adipose tissue | 1 | dx.doi.org/10.17504/protocols.io.w2vfge6 | https://www.protocols.io/view/pseudorabies-virus-prv-injection-into-interscapula-w2vfge6 | Heike Muenzberg | TITLE: Pseudorabies Virus (PRV) injection into interscapular brown adipose tissue
AUTHORS: Heike Muenzberg
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Pseudorabies Virus (PRV) injection into interscapular brown adipose tissue.</div></div>
[STEPS]
?. Place a sterile towel on the surgery platform... | ["Place a sterile towel on the surgery platform over the heating mat, another sterile towel in the anesthesia induction chamber.\n- All PRV work is performed in BSL2 laboratories, and all PRV injected mice are kept in BSL2 housing till euthanasia.- All containers that are in contact with virus waste need to be properly... |
69,687 | MagAttract + Metapolyzyme metagenomic gDNA extraction from urine | 4 | null | https://www.protocols.io/view/magattract-metapolyzyme-metagenomic-gdna-extractio-cgaxtsfn | Natalie Ring | TITLE: MagAttract + Metapolyzyme metagenomic gDNA extraction from urine
AUTHORS: Natalie Ring
[DESCRIPTION]
A protocol for the metagenomic extraction of bacterial DNA from urine samples (optimised using dog urine), for use in a rapid diagnostics pipeline. At the end of the protocol, the DNA is cleaned up and ready for... | ["[Extended pre-lysis spin down] Pellet 2x 1.5 ml aliquots of urine in 1.5 ml tubes by centrifuging at maximum speed (13,000 RPM) for 20 minutes, then discard supernatant\n\n3 mL \n\n\n16,000 x g, 20 min, RT Room temperature", "[Metapolyzyme & Proteinase K Lysis] Resuspend cell pellets (which might be invisible) an... |
69,905 | ONT Q20+ (V12) Adapter Ligation for Fungal DNA Barcoding | 4 | dx.doi.org/10.17504/protocols.io.dm6gpb5zdlzp/v3 | https://www.protocols.io/view/ont-q20-v12-adapter-ligation-for-fungal-dna-barcod-cghrtt56 | Stephen Douglas Russell, Stephen Douglas Russell | TITLE: ONT Q20+ (V12) Adapter Ligation for Fungal DNA Barcoding
AUTHORS: Stephen Douglas Russell, Stephen Douglas Russell
[DESCRIPTION]
This process will take your A-tailed library and add the nanopore adapters. Simply combine several chemicals for a single reaction and do a bead cleanup.
Tested with:
Flowcel... | ["[Adapter Ligation] Spin down the Adapter Mix H (AMX H) and Quick T4 Ligase, and place on ice.\n\nAMX H - \nQuick T4 Ligase -", "[Adapter Ligation] Thaw Ligation Buffer (LNB) at room temperature, spin down and mix by pipetting. Due to viscosity, vortexing this buffer is ineffective. Place on ice immediately after tha... |
85,596 | Sample preparation and imaging for large scale 3D spectral confocal imaging of tissues | 1 | dx.doi.org/10.17504/protocols.io.bp2l6x1prlqe/v1 | https://www.protocols.click/view/sample-preparation-and-imaging-for-large-scale-3d-cxt4xnqw | Tarek Ashkar, Michael Ferkowicz | TITLE: Sample preparation and imaging for large scale 3D spectral confocal imaging of tissues
AUTHORS: Tarek Ashkar, Michael Ferkowicz
[DESCRIPTION]
Frozen participant samples are first sized and preserved for high-resolution 3D imaging. Preserved biopsy sections are imaged, without staining, to understand the sample’... | ["[Label Free Imaging of Tissue] Mount sample on uncharged slide in PBS using prepositioned piece of spacer tape to support coverslip.", "[Label Free Imaging of Tissue] Gently push coverslip to the sample and seal with rubber cement dispensed from a syringe and 16 G needle.", "[Label Free Imaging of Tissue] Configure t... |
51,019 | Terra Registration - Linking GCP Credits to the Terra Platform | 5 | null | https://www.protocols.io/view/terra-registration-linking-gcp-credits-to-the-terr-bv3jn8kn | Anusha Ginni, Jill V Hagey, Technical Outreach and Assistance for States Team | TITLE: Terra Registration - Linking GCP Credits to the Terra Platform
AUTHORS: Anusha Ginni, Jill V Hagey, Technical Outreach and Assistance for States Team
[DESCRIPTION]
This Terra registration protocol provides step-by-step guidance to register and activate a Google account as well enable Google Cloud free credits... | ["[Setup Terra and Google Cloud Accounts] To set up and link google account to Terra follow the instructions from the links below.\n\nFor detailed step-by-step instructions scroll down to Step 2.", "[Setup Terra and Google Cloud Accounts] Setting up the Google Cloud Platform (GCP) free credits to use in Terra \n\nIf yo... |
null | null | null | dx.doi.org/10.17504/protocols.io.ewabfae | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
A collection of protocols designed to guide the user in processing a viral metagenome from raw sequence data to assembly, and subsequent analysis. The user uses <em>actual</em> reads from <a href="https://github.com/MicroB3-IS/osd-analysis" target="_blank">Ocean Sampling Day (20... | [] |
13,264 | Second-derivative UV Spectroscopy | null | dx.doi.org/10.17504/protocols.io.q7qdzmw | null | Moriah Beck | TITLE: Second-derivative UV Spectroscopy
AUTHORS: Moriah Beck
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Second-derivative spectroscopy has proven to be an effective analytical tool because of its ability to resolve overlapping bands in the normal spectrum.</div><div class = "text-block">This p... | ["[Protein Concentrations]\nDetermine protein concentrations either using absorption coefficients (Abs @ 280 nm) or using BCA, Bradford, etc. colorimetric assays.Proteins should be used at concentration where A280 ~ 0.2", "[UV Scan]\nScan from 270-300 nm", "[Obtain Second-derivative]\nCopy UV scan data from instrument ... |
103,193 | Lentivirus Production and Astrocyte Transduction | 0 | dx.doi.org/10.17504/protocols.io.ewov1934klr2/v1 | https://www.protocols.io/view/lentivirus-production-and-astrocyte-transduction-dgzz3x76 | Shiyi Wang | TITLE: Lentivirus Production and Astrocyte Transduction
AUTHORS: Shiyi Wang
[DESCRIPTION]
Lentivirus Production and Astrocyte Transduction
[STEPS]
1. 1. **Materials Required**
1.1. - pLKO.1 shRNA Puro targeting plasmid (for astrocyte transduction)
1.2. - Envelope plasmid (VSVG)
1.3. - Packaging plasmid (dR8.91)
1.4. ... | ["1. **Materials Required**", "- pLKO.1 shRNA Puro targeting plasmid (for astrocyte transduction)", "- Envelope plasmid (VSVG)", "- Packaging plasmid (dR8.91)", "- HEK293T cells", "- X-tremeGENE transfection reagent (Roche)", "- Astrocyte growth media (AGM)", "- Puromycin (for selection)", "2. **Transfection of HEK293T... |
93,626 | Change in Research Metrics and ResearchGate dental users during the COVID-19 pandemic | 1 | null | https://www.protocols.io/view/change-in-research-metrics-and-researchgate-dental-c7n2zmge | Maha El Tantawi | TITLE: Change in Research Metrics and ResearchGate dental users during the COVID-19 pandemic
AUTHORS: Maha El Tantawi
[DESCRIPTION]
The field of dental science has experienced significant growth, with a substantial increase in research publications, particularly during the COVID-19 pandemic. This growth is evident in ... | ["[Background and Rationale] The field of dental science has witnessed a\nnotable surge in growth, marked by an exponential increase in research\npublications. This expansion wasparticularly pronounced in the context of the COVID-19 pandemic, which has led academics to transfer at least part of their academic and resea... |
54,686 | Depression Detection Algorithm | 3 | dx.doi.org/10.17504/protocols.io.bzm6p49e | https://www.protocols.io/view/depression-detection-algorithm-bzm6p49e | Umme Marzia Haque | TITLE: Depression Detection Algorithm
AUTHORS: Umme Marzia Haque
[DESCRIPTION]
The study has used data from YMM. The Yes/No variables that had a low correlation with target variable have been removed. To extract the most relevant features , the high correlated variables with the target variable , the Boruta method wa... | [] |
56,692 | Electroporation transformation of Ostreococcus tauri | 4 | dx.doi.org/10.17504/protocols.io.b3kuqkww | https://www.protocols.io/view/electroporation-transformation-of-ostreococcus-tau-b3kuqkww | Anbarasu Karthikaichamy, Shikha Adhikari | TITLE: Electroporation transformation of Ostreococcus tauri
AUTHORS: Anbarasu Karthikaichamy, Shikha Adhikari
[DESCRIPTION]
The protocol describes steps for electroporation transformation of Ostreococcus taurii. This protocol is a direct version of published transformation protocol, https://dx.doi.org/10.3791/4074 b... | ["[Preparing Ostreococcus taurii culture] Culture Ostreococcus taurii cells in K-media. The protocol used to prepare K-media is \ndx.doi.org/10.17504/protocols.io.brv7m69n", "[Preparing Ostreococcus taurii culture] Sub-culture Ostreococcus taurii cells in K-media at 1% dilution every 10 days and grow in a plant growth ... |
23,015 | Mouse Stellate- Intracellular recording | null | dx.doi.org/10.17504/protocols.io.2qfgdtn | null | John Tompkins, Mike Andresen | TITLE: Mouse Stellate- Intracellular recording
AUTHORS: John Tompkins, Mike Andresen
[STEPS]
?. See mouse stellate iosolation protocol
?. Isolated mouse stellate pinned to clean Sylgard perti dish
?. Continuously superfuse isolated stellate (6–7 ml/min) with PSS (32–35°C).
?. Impale individual neurons using microelect... | ["See mouse stellate iosolation protocol", "Isolated mouse stellate pinned to clean Sylgard perti dish", "Continuously superfuse isolated stellate (6–7 ml/min) with PSS (32–35°C).", "Impale individual neurons using microelectrodes filled with either 2M KCl (60–120 MΩ) or 2M KCl + 2% Neurobiotin (80-160 MΩ; Vector Labs)... |
86,481 | Calculating mitochondrial protein solubility | 5 | dx.doi.org/10.17504/protocols.io.81wgbxx7nlpk/v1 | https://www.protocols.io/view/calculating-mitochondrial-protein-solubility-cyprxvm6 | Louise Uoselis | TITLE: Calculating mitochondrial protein solubility
AUTHORS: Louise Uoselis
[DESCRIPTION]
Protocol for generating solubility calculations for mitochondrial proteins from starting 'soluble' and 'insoluble' fraction mass spectrometry data, using MaxQuant and Perseus software pipelines.
[STEPS]
1. Process raw instrument... | ["Process raw instrument files using MaxQuant (protocol performed with v1.6.17) with the Andromeda search engine, searching against the Uniprot human data base containing reviewed and canonical isoform variants in the FASTA format, with recombinant Ag85A sequence added as a custom entry to the human database", "Set the... |
39,442 | Reverse transcription of complementary DNA | 4 | dx.doi.org/10.17504/protocols.io.birskd6e | https://www.protocols.io/view/reverse-transcription-of-complementary-dna-birskd6e | Madeline M Glennon, Austin Skinner, Mara Krutsinger, Marino Resendiz | TITLE: Reverse transcription of complementary DNA
AUTHORS: Madeline M Glennon, Austin Skinner, Mara Krutsinger, Marino Resendiz
[STEPS]
?. [Reverse Transcription using AMV RT]
Obtain an RNase -free microcentrifuge tube Add the following components in the described order:-Water -10 X Buffer -5'-P32 Radiolabeled DNA -RN... | ["[Reverse Transcription using AMV RT]\nObtain an RNase -free microcentrifuge tube Add the following components in the described order:-Water -10 X Buffer -5'-P32 Radiolabeled DNA -RNA\n400 µl\n15 µl\n[50 mM Tris-acetate, 75 mM Potassium Acetate, 8 mM Magnesium Acetate, 10 mM DTT (pH 8.3)]\n[< 1 pmol]\n[app. 2 pmol]", ... |
66,336 | Diaetoxil Kapseln Avis- FRance Prix, Medical Fake Avantage | 1 | dx.doi.org/10.17504/protocols.io.yxmvmn4nog3p/v1 | https://www.protocols.io/view/diaetoxil-kapseln-avis-france-prix-medical-fake-av-ccz8sx9w | health | TITLE: Diaetoxil Kapseln Avis- FRance Prix, Medical Fake Avantage
AUTHORS: health
[DESCRIPTION]
Diaetoxil Avis - Diaetoxil est l'un des suppléments de perte de poids les plus fiables. Il peut être utilisé par n'importe qui, quel que soit son âge, car il est extrêmement sûr à utiliser.
[STEPS]
1. Diaetoxil Avis - Di... | ["Diaetoxil Avis - Diaetoxil est l'un des suppléments de perte de poids les plus fiables. Il peut être utilisé par n'importe qui, quel que soit son âge, car il est extrêmement sûr à utiliser. Diaetoxil Ingrédients, Résultats Une alimentation propre se concentre sur la consommation d'aliments entiers peu transformés et ... |
26,324 | Defrost a C. elegans strain from the -80 C freezer | null | dx.doi.org/10.17504/protocols.io.5xug7nw | null | Cristian Riccio | TITLE: Defrost a C. elegans strain from the -80 C freezer
AUTHORS: Cristian Riccio
[STEPS]
?. Take the frozen C. elegans tube out of the -80 C freezer.
?. Put the tube in a foam floater.
?. Put the foam floater on the surface of lukewarm water in a beaker.
?. When the content of the tube has defrosted (should take onl... | ["Take the frozen C. elegans tube out of the -80 C freezer.", "Put the tube in a foam floater.", "Put the foam floater on the surface of lukewarm water in a beaker.", "When the content of the tube has defrosted (should take only a few minutes), pour the contents onto an NGM plate seeded with bacteria.", "Put the plate ... |
63,876 | Martha MacCallum CBD Gummies Benefits,Ingredients,side effects and Is it legitor Does it Really Work Shark TankReviews [HOAX OR SCAM]{Update 2022}-, What To Know Before Using It?? | 1 | dx.doi.org/10.17504/protocols.io.5jyl89ky6v2w/v1 | https://www.protocols.io/view/martha-maccallum-cbd-gummies-benefits-ingredients-camcsc2w | kaurnikki | TITLE: Martha MacCallum CBD Gummies Benefits,Ingredients,side effects and Is it legitor Does it Really Work Shark TankReviews [HOAX OR SCAM]{Update 2022}-, What To Know Before Using It??
AUTHORS: kaurnikki
[DESCRIPTION]
Martha MacCallum CBD Gummies
[STEPS]
1. Martha MacCallum CBD GummiesProduct Review: —Martha MacC... | ["Martha MacCallum CBD GummiesProduct Review: —Martha MacCallum CBD GummiesUsed For: — 🔶Pain Relief\n🔶Health Benefits\n🔶Burn excess fat\n🔶Better gut health & promote digestion\n🔶Improves heart health\n🔶Control your appetite\n➢ Where to Buy - 🔶Click Here to Rush Your Order from the Official Website\n➤ Price (for... |
106,891 | Endo H/ PNGase F Assay for JESS Automated Western Blot | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qzyjl1y/v1 | https://www.protocols.io/view/endo-h-pngase-f-assay-for-jess-automated-western-b-dkmj4u4n | Charis Ma, Yu Chen, sidranse | TITLE: Endo H/ PNGase F Assay for JESS Automated Western Blot
AUTHORS: Charis Ma, Yu Chen, sidranse
[DESCRIPTION]
This protocol details an optimized Endo H and PNGase F digestion assay that produces samples that can be run with the JESS Automated Western Blot System.
[STEPS]
SECTION: Glycoprotein Denaturation
1. In... | ["[Glycoprotein Denaturation] In an eppendorf tube, add 40 µg protein, 2 µL 10X Glycoprotein Denaturing Buffer, and water for a total reaction volume of 20 µL . Mix well.", "[Glycoprotein Denaturation] Denature glycoprotein by heating reaction at 100 °C for 10 min .", "[Glycoprotein Denaturation] Split reaction volume... |
44,747 | PBMC- 01a - Isolation of Human PBMC from Buffy Coat | 4 | dx.doi.org/10.17504/protocols.io.bpxjmpkn | https://www.protocols.io/view/pbmc-01a-isolation-of-human-pbmc-from-buffy-coat-bpxjmpkn | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: PBMC- 01a - Isolation of Human PBMC from Buffy Coat
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">List of published work using th... | ["Put the needed amount of blood sample from buffy coat into a 50 ml conical tube.", "Add an equal volume of PBS 1X and mix well.\n{\"blocks\":[{\"key\":\"6d8lo\",\"text\":\"This recepe is used in the following protocols:\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"k... |
18,539 | 0.1% Cresyl Violet Stain (pH 4.3) | null | dx.doi.org/10.17504/protocols.io.wcjfaun | null | Shaina Robbins, Sean Nieves | TITLE: 0.1% Cresyl Violet Stain (pH 4.3)
AUTHORS: Shaina Robbins, Sean Nieves
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Solution Prep]
Measure of ddH2O or molecular grade H2O and pour into a glass beaker
500 ml
?. [Solution Prep]
Aliquot of cresyl violet acetate into the glass beaker from the abov... | ["[Solution Prep]\nMeasure of ddH2O or molecular grade H2O and pour into a glass beaker\n500 ml", "[Solution Prep]\nAliquot of cresyl violet acetate into the glass beaker from the above step\n0.5 g\nCauses skin irritationMay cause respiratory irritationCauses serious eye irritation", "[Solution Prep]\nPut a magnetic ... |
89,431 | Filter-paper based PURExpress detection of ZikV and CoV2 RNA by enhancer toehold (TacToe) sensors | 4 | dx.doi.org/10.17504/protocols.io.q26g7p3p1gwz/v1 | https://www.protocols.io/view/filter-paper-based-purexpress-detection-of-zikv-an-c3jxykpn | Tanvi Kale, Chaitanya Anil Athale | TITLE: Filter-paper based PURExpress detection of ZikV and CoV2 RNA by enhancer toehold (TacToe) sensors
AUTHORS: Tanvi Kale, Chaitanya Anil Athale
[DESCRIPTION]
This method details the approach to using in vitro transcription-translation (TxTr) systems based on PURExpress adsorbed on a paper disc. These to run a to... | ["[Reaction Kinetics using PURExpress] BSA treated paper disk preparation:\n1) Make 5% BSA solution in ultrapure water\n2) Soak filter paper disc (Whatmann filter paper 42, ashless, dm 42.5) in BSA solution, keep it at room temperature for overnight in a beaker \n3) Rinse the filter paper using ultrapure water (repeat ... |
28,027 | Phage lambda genome concatemerization | null | dx.doi.org/10.17504/protocols.io.7k3hkyn | null | Marijn Ceelen | TITLE: Phage lambda genome concatemerization
AUTHORS: Marijn Ceelen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to create concatemers of phage genomes. This is done by ligating phage lambda genomes using the overhangs at the cos site with T4 ligase. </div></div>
[STEPS]
?.... | ["Add the following ingredients in a microcentrifuge tube. AB1Ingredient Amount 2Ligation buffer 10X 2 µL 3DNA 6 µg 4PEG 4000 (40% W/W) 2.5 µL 5T4 DNA ligase 1 µ L (400 Weiss units) 6Water To 20 µL \nAB1Ingredient... |
62,254 | Ketosium XS Keto Reviews : How To Use It? | 1 | dx.doi.org/10.17504/protocols.io.261gen26yg47/v1 | https://www.protocols.io/view/ketosium-xs-keto-reviews-how-to-use-it-b82nryde | ketosiumketoreviews | TITLE: Ketosium XS Keto Reviews : How To Use It?
AUTHORS: ketosiumketoreviews
[DESCRIPTION]
Ketosium XS Keto Reviews : How To Use It?
[STEPS]
1. Ketosium XS Keto Reviews : How To Use It?
Are you floundering with weight problems and facing health problems, including feeling bad and losing energy and vitality? Do yo... | ["Ketosium XS Keto Reviews : How To Use It?\nAre you floundering with weight problems and facing health problems, including feeling bad and losing energy and vitality? Do you no longer feel at ease with your standard of living as your energy, health, and vitality dwindle, and want to restore your sound health and vibra... |
null | null | null | dx.doi.org/10.17504/protocols.io.gtsbwne | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol for separating total RNA using denaturing formaldehyde agarose gel electrophoresis.</p>
<p>This method can be used to separate larger RNAs in a range of 400-6000 nt, either for quality control or downstream Northern Blot Analysis.</p>
<p> </p>
[GUIDELINES]
<p>Always... | [] |
38,207 | Cytoplasmic pH measurements with pHluorin | 1 | dx.doi.org/10.17504/protocols.io.bhi7j4hn | https://www.protocols.io/view/cytoplasmic-ph-measurements-with-phluorin-bhi7j4hn | Ekaterina Krasnopeeva, Teuta Pilizota | TITLE: Cytoplasmic pH measurements with pHluorin
AUTHORS: Ekaterina Krasnopeeva, Teuta Pilizota
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Single-cell </span><span style = "font-style:italic;">in vivo </span><span>cytoplasmic pH measurments with genetically encoded pHluorin sensor</span>... | ["[Sample preparation]\nGrow cells to the desired OD according to a chosen growth protocol.", "[Sample preparation]\nPrepare a tunnel-slide/flow cell and attach cells to the coverslip with the preferred surface attachment method (Wang et al. 2019)\nIt is preferable to use a non-fluorescent medium for imaging. If needed... |
83,812 | LB Broth | 4 | dx.doi.org/10.17504/protocols.io.yxmvm375nl3p/v1 | https://www.protocols.click/view/lb-broth-cv4cw8sw | sgoodwin | TITLE: LB Broth
AUTHORS: sgoodwin
[DESCRIPTION]
Process for producing LB broth growth medium
[STEPS]
SECTION: Making the LB broth
1. Dilute 20 g of LB powder in 800 mL distilled water
SECTION: Making the LB broth
2. Stir until fully dissolved with magnetic stirrer
SECTION: Making the LB broth
3. Transfer to a Duran ... | ["[Making the LB broth] Dilute 20 g of LB powder in 800 mL distilled water", "[Making the LB broth] Stir until fully dissolved with magnetic stirrer", "[Making the LB broth] Transfer to a Duran flask, and sterilise by autoclaving"] |
88,826 | 13158 - Rooting Medium | 4 | null | https://www.protocols.io/view/13158-rooting-medium-c2y2yfye | leiboffs | TITLE: 13158 - Rooting Medium
AUTHORS: leiboffs
[DESCRIPTION]
This is part of the Leiboff Lab maize transformation protocol for somatic embryogenesis of B104 immature embryos. This protocol is a combination of Chen et al. 2022 and Kang et al. 2022 with some modifications based on material availability. This protocol i... | ["[Planning] Estimate the volume of 13158 you will need based on the following:\n\n \n\nMake sure to round up! Check the table below to plan your media", "[Mixing Heat-Stable Ingredients] Retrieve the following heat-stable ingredients:\nMurashige & Skoog Basal Salt Mixture, 'Basal MS' - Stored in Main Lab, 4C Refriger... |
null | null | null | dx.doi.org/10.17504/protocols.io.jtjcnkn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
49,979 | Lysosomal flux assay | 1 | dx.doi.org/10.17504/protocols.io.q26g786j3lwz/v1 | https://www.protocols.io/view/lysosomal-flux-assay-bu23nygn | Giacomo Monzio Compagnoni | TITLE: Lysosomal flux assay
AUTHORS: Giacomo Monzio Compagnoni
[DESCRIPTION]
This protocol details the assessment of the autophagic flux in cells, by evaluating LC3II and p62 amount before and after bafilomycin treatment.
[STEPS]
SECTION: Bafilomycin treatment
1. Change medium to complete medium + bafilomycin (eg 50 ... | ["[Bafilomycin treatment] Change medium to complete medium + bafilomycin (eg 50 nanomolar (nM), 100 nanomolar (nM), 200 nanomolar (nM)).", "[Bafilomycin treatment] Treat control wells with complete medium + DMSO (eg 500X, to match bafilomycin dilution) (bafilomycin aliquots are resuspended in DMSO).", "[Bafilomycin tre... |
53,700 | ADE 2022 Day 3: Morphological Taxonomy | 3 | dx.doi.org/10.17504/protocols.io.36wgq4z1kvk5/v2 | https://www.protocols.io/view/ade-2022-day-3-morphological-taxonomy-bypcpviw | Tom Little | TITLE: ADE 2022 Day 3: Morphological Taxonomy
AUTHORS: Tom Little
[DESCRIPTION]
Day 3: ADE practical instructions for morphological identifications
[STEPS] | [] |
78,765 | 3D Mesh Cleanup Tutorial: Fossil Crab (Advanced) | 1 | dx.doi.org/10.17504/protocols.io.3byl4j68olo5/v1 | https://www.protocols.io/view/3d-mesh-cleanup-tutorial-fossil-crab-advanced-cq6mvzc6 | Elizabeth G. Clark, Kelsey M Jenkins, Craig R Brodersen | TITLE: 3D Mesh Cleanup Tutorial: Fossil Crab (Advanced)
AUTHORS: Elizabeth G. Clark, Kelsey M Jenkins, Craig R Brodersen
[DESCRIPTION]
This protocol details about 3D mesh cleanup tutorial of fossil crab (Advanced).
[GUIDELINES]
Skills developed: Advanced mesh editing
3D meshes of fossil specimens extracted directly... | ["[Skills developed: Advanced mesh editing] Import the crab into Meshlab by following Part 1 in the Cupule Cleanup Tutorial.", "[Remove External Debris] The imported mesh includes the crab, but it also includes other small isolated meshes that are 3D reconstructions of bits of the matrix. Remove this external debris by... |
70,382 | OT-2 Protocol to transfer volume from several plates to a single plate | 5 | dx.doi.org/10.17504/protocols.io.6qpvr4o62gmk/v1 | https://www.protocols.io/view/ot-2-protocol-to-transfer-volume-from-several-plat-cgyntxve | Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno | TITLE: OT-2 Protocol to transfer volume from several plates to a single plate
AUTHORS: Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno
[DESCRIPTION]
This protocol is meant to transfer samples from different plates to a single plate. The output of running this script will be the final plate(s) and the correspond... | ["[Files Preparation] Preparing Customized Template\n\nPreparing the template (.csv file) with the specific variables for each experiment.\n\nHere we attach one excel with the following sheets:\nTemplate to use in protocol\nExplanation of each variable\nAn Example", "[Running Protocol] Setting Labware", "[After-running... |
37,465 | Radioactive Labeling with T4 PNK (M0201) | 1 | dx.doi.org/10.17504/protocols.io.bgtzjwp6 | https://www.protocols.io/view/radioactive-labeling-with-t4-pnk-m0201-bgtzjwp6 | New England Biolabs | TITLE: Radioactive Labeling with T4 PNK (M0201)
AUTHORS: New England Biolabs
[DESCRIPTION]
Radioactive Labeling with T4 Polynucleotide Kinase.
[STEPS]
1. Set-up the following reaction:
2. Incubate at 37 °C for 30 min.
3. Heat inactivate by incubating at 65 °C for 20 min. | ["Set-up the following reaction:", "Incubate at 37 °C for 30 min.", "Heat inactivate by incubating at 65 °C for 20 min."] |
101,941 | Nissl Counterstaining - Cresyl Violet | 0 | dx.doi.org/10.17504/protocols.io.3byl49wmrgo5/v1 | https://www.protocols.io/view/nissl-counterstaining-cresyl-violet-dfsv3ne6 | Núria Peñuelas, Ariadna Laguna, Miquel Vila | TITLE: Nissl Counterstaining - Cresyl Violet
AUTHORS: Núria Peñuelas, Ariadna Laguna, Miquel Vila
[DESCRIPTION]
Nissl Counterstaining with Cresyl Violet for histological sections
[STEPS]
SECTION: Delipidation Step
1. Ethanol 95%, 5min
SECTION: Cresyl Violet staining
7. Cresyl Violet solution, 1min
SECTION: Cresyl Vi... | ["[Delipidation Step] Ethanol 95%, 5min", "[Cresyl Violet staining] Cresyl Violet solution, 1min", "[Cresyl Violet staining] dH2O, 15sec or less (quick rinse)", "[Cresyl Violet staining] dH2O, 2min", "[Differentiation] Ethanol 70%+Acid Acetic*, few seconds (control differentiation yourself). (*add 2-3 drops\nof acetic ... |
81,380 | Electroforesis de proteínas y Western blot DENV | 1 | dx.doi.org/10.17504/protocols.io.kxygx963dg8j/v1 | https://www.protocols.click/view/electroforesis-de-prote-nas-y-western-blot-denv-ctqcwmsw | Delia Piedad Recalde-Reyes, Juliana Lopez Calderon | TITLE: Electroforesis de proteínas y Western blot DENV
AUTHORS: Delia Piedad Recalde-Reyes, Juliana Lopez Calderon
[DESCRIPTION]
Una prueba tipo Western Blot (también conocida como inmunotransferencia) es una técnica utilizada para detectar proteínas específicas en una muestra. El procedimiento consiste en separar las... | ["Gel de electroforesis de proteínas (Denaturante) al 10% (Volumenes suficientes para 2 geles)\n\nPreparar gel de resolución empleando \n\nAcrilamida bis acrilamida 40% 2.5 mL\n1.5M Tris HCl pH 8.8 2.5 mL\nSDS 10% 100 µL\nAgua destilada ... |
44,019 | H&E staining on paraffin sections | 6 | dx.doi.org/10.17504/protocols.io.4r3l24on4g1y/v1 | https://www.protocols.io/view/h-amp-e-staining-on-paraffin-sections-bn8tmhwn | Mel Feany | TITLE: H&E staining on paraffin sections
AUTHORS: Mel Feany
[DESCRIPTION]
This protocol describes H&E staining on fly heads paraffin sections for neuronal counting experiments.
[STEPS]
SECTION: For neuronal counting experiments
1. Fix fly heads in 4 %.
SECTION: For neuronal counting experiments
2. Embed in para... | ["[For neuronal counting experiments] Fix fly heads in 4 %.", "[For neuronal counting experiments] Embed in paraffin.", "[For neuronal counting experiments] Cut 4 micron sections.", "[For neuronal counting experiments] Dry at 42 °C .", "[For neuronal counting experiments] Deparaffinize and bring through ethanols to ... |
null | null | null | dx.doi.org/10.17504/protocols.io.n62dhge | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Validated with : rodent liver, kidney, eye, skeletal muscles, adrenal gland. </p>
[BEFORE_START]
<ol>
<li>Prepare the lysis buffer on the day of the extraction by adding 1% of beta-mercaptoethanol to the total needed RLT volume.</li>
<li>Use 1000ul of RLT+1% β-ME per sample ... | [] |
85,251 | Eastern Hemlock Tissue Collection for DNA | 4 | null | https://www.protocols.click/view/eastern-hemlock-tissue-collection-for-dna-cxhbxj2n | Karl Fetter | TITLE: Eastern Hemlock Tissue Collection for DNA
AUTHORS: Karl Fetter
[DESCRIPTION]
Steps for collecting tissue from Eastern Hemlocks for DNA analysis. The Plant Computational Genomics lab is conducting a landscape genomics study of climate adaptation in the Eastern Hemlock. This protocol is a step-by-step guide to co... | ["[Introduction] This protocol is intended for field collection of leaf tissue for the Eastern Hemlock conservation genomics project sponsored by the Plant Computational Genomics lab at the University of Connecticut. The goal of the project is to identify climate adapted genomic variation for seed banking and potential... |
93,919 | Endocytosis and internalization assay in primary neuronal culture | 4 | dx.doi.org/10.17504/protocols.io.4r3l22j5ql1y/v1 | https://www.protocols.io/view/endocytosis-and-internalization-assay-in-primary-n-c7x7zprn | Arpine Sokratian, andrew.west west | TITLE: Endocytosis and internalization assay in primary neuronal culture
AUTHORS: Arpine Sokratian, andrew.west west
[DESCRIPTION]
This protocol outlines a method for quantifying endocytosis in primary neuronal cultures, specifically focusing on different a-synuclein fibril structures. By utilizing pH-sensitive dye co... | ["[Preparation of endocytosis inhibitors] Prepare stock solution of the endocytosis inhibitors in DMSO:", "[Preparation of endocytosis inhibitors] At the day of the experiment, thaw down the stock solutions in a water bath and dilute the drugs to reach concentrations:", "[Preparation of endocytosis inhibitors] For the ... |
null | null | null | dx.doi.org/10.17504/protocols.io.cdqs5v | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
A set of Stellaris FISH Probes comprises up to 48 singly labeled oligonucleotides designed to selectively bind to targeted transcripts. Stellaris FISH Probes bound to target RNA produce fluorescent signals that permit detection of single RNA molecules as diffraction-limited spot... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.iksccwe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.jk5cky6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Dear researcher,</p>
<p>This protocol gives you the steps to start an MRI project at the Spinoza Centre Roeterseiland. </p>
<p>If you haven't had contact with Steven Scholte or Tinka Beemsterboer, make sure to contact them before completing this protocol. </p>
<p> </p>
<p>You... | [] |
90,473 | C-SOP-901: Preparation of DNA Isolates for Domestic and Overseas Transport | 4 | null | https://www.protocols.io/view/c-sop-901-preparation-of-dna-isolates-for-domestic-c4khyut6 | Mihir Kekre, Ben Pascoe | TITLE: C-SOP-901: Preparation of DNA Isolates for Domestic and Overseas Transport
AUTHORS: Mihir Kekre, Ben Pascoe
[DESCRIPTION]
DNA eluted in nuclease-free water or low-TE solution is stable enough to be shipped at ambient temperatures overseas. This characteristic of DNA becomes vital when dealing with logistics and... | ["[Before Starting] Ensure that all of the materials listed are available before you begin aliquoting and packaging DNA samples for transport. Contact your local courier provider to obtain consignment containers.", "[Aliquoting DNA into a 96-well plate] Using filter tips, transfer the required amount of each DNA sample... |
43,654 | Sequencing for Bark beetles and associated fungi | 3 | dx.doi.org/10.17504/protocols.io.bnveme3e | https://www.protocols.io/view/sequencing-for-bark-beetles-and-associated-fungi-bnveme3e | Jiri Hulcr, Sawyer Adams | TITLE: Sequencing for Bark beetles and associated fungi
AUTHORS: Jiri Hulcr, Sawyer Adams
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The purpose of this protocol is to describe the submission for sequencing at the UF Forest Entomology Lab.</div><div class = "text-block"><span>This protocol is p... | [] |
35,127 | An optimized protocol for sequencing mammalian roadkill tissues with Oxford Nanopore Technology (ONT) | null | dx.doi.org/10.17504/protocols.io.beixjcfn | null | Marie-Ka Tilak, Rémi Allio, Frédéric Delsuc | TITLE: An optimized protocol for sequencing mammalian roadkill tissues with Oxford Nanopore Technology (ONT)
AUTHORS: Marie-Ka Tilak, Rémi Allio, Frédéric Delsuc
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol was developed to optimize DNA sequencing from mammalian roadkill tissue samp... | ["Tissue sampling and preservationTissues were preserved in RNAlater Thermo Fisher® or 95% EtOH. We have observed that RNAlater preservation was often better to conserve DNA from internal organ tissues than 95% EtOH (Figure 1).", "Tissue preparationBefore the lysis step, put the tissue in a new fresh preservation li... |
94,706 | Arabidopsis thaliana detached leaf assay for the disease assessment of Fusarium graminearum infection | 4 | dx.doi.org/10.17504/protocols.io.261gedmwov47/v1 | https://www.protocols.io/view/arabidopsis-thaliana-detached-leaf-assay-for-the-d-c8qszvwe | Victoria J Armer, Michael J. Deeks, Kim E. Hammond-Kosack | TITLE: Arabidopsis thaliana detached leaf assay for the disease assessment of Fusarium graminearum infection
AUTHORS: Victoria J Armer, Michael J. Deeks, Kim E. Hammond-Kosack
[DESCRIPTION]
Arabidopsis thaliana (At), as the plant model organism, has the advantage of a bank of genotypes in stock centres worldwide. This... | ["[1.1.\tPreparation of ½ MS plates for At germination] This step was found necessary to eliminate plate contamination from other fungal and bacterial species during seed germination on ½ MS agar plates. Unlike usual ½ MS preparations, this one\ndoes not contain sucrose to limit contamination.\n\nThis can be done ahead... |
59,679 | Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP | 1 | dx.doi.org/10.17504/protocols.io.q26g74169gwz/v1 | https://www.protocols.io/view/processing-frozen-cells-for-population-scale-oxfor-b6h7rb9n | Pilar Alvarez Jerez, Kimberley J Billingsley, Cornelis Blauwendraat, on behalf of the CARD Long-read Team | TITLE: Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP
AUTHORS: Pilar Alvarez Jerez, Kimberley J Billingsley, Cornelis Blauwendraat, on behalf of the CARD Long-read Team
[DESCRIPTION]
Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP
At... | ["Part 1: Extracting Cell DNA (~2 hours per 8 samples)\n\nFrom: Circulomics HMW DNA Extraction Cultured Cells Protocol\n\nKit required: Nanobind CBB Big DNA Kit or Nanobind Tissue Big DNA Kit\nCell Input Requirements: 1x106 – 5x106 diploid human cells or equivalent \nCell counts should be accurately determined using a ... |
89,089 | QIAGEN QIAseq FX Protocol | 1 | dx.doi.org/10.17504/protocols.io.3byl4q562vo5/v1 | https://www.protocols.io/view/qiagen-qiaseq-fx-protocol-c289yhz6 | Emily Gailey | TITLE: QIAGEN QIAseq FX Protocol
AUTHORS: Emily Gailey
[DESCRIPTION]
QIAseq Normalizer Kits provide a fast and easy way to normalize and pool NGS libraries for
Illumina, while skipping library qualification and quantification steps. QIAseq Normalizer will
accurately normalize over a broad range of library concentratio... | ["[Procedure] Program a thermocycler according to Table 2 using the predetermined FX fragmentation\ntime for step 2. Be sure to use the instrument’s heated lid, and if possible, set the\ntemperature of the heated lid to 70°C.\n\nTable 2. Input DNA (20 pg –1000 ng) free of EDTA, Buffer EB, or in 0.1x T", "[Procedure] St... |
91,668 | ELISA | 1 | dx.doi.org/10.17504/protocols.io.kqdg3xw67g25/v1 | https://www.protocols.io/view/elisa-c5ruy56w | Pranay Srivastava | TITLE: ELISA
AUTHORS: Pranay Srivastava
[DESCRIPTION]
This protocol is to determine the levels of IL-1β and TNF-α in plasma and brain (Srivastava et al. 2020).
[STEPS]
1. Blood samples from all the treatment groups were collected through cardiac puncture in 40 mM EDTA and plasma was collected by centrifugation.
2. T... | ["Blood samples from all the treatment groups were collected through cardiac puncture in 40 mM EDTA and plasma was collected by centrifugation.", "The plasma samples were immediately transferred to dry ice and then stored at -80°C till further analysis.", "A small fraction of ventral midbrain tissue homogenate prepared... |
36,645 | Imaging Mass Cytometry Compensation Slide Preparation | null | dx.doi.org/10.17504/protocols.io.bf2djqa6 | https://www.protocols.io/view/imaging-mass-cytometry-compensation-slide-preparat-bf2djqa6 | Michelle Daniel, Marda Jorgensen | TITLE: Imaging Mass Cytometry Compensation Slide Preparation
AUTHORS: Michelle Daniel, Marda Jorgensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SOP describes the preparation of compensation slides with single antibodyconjugate</div><div class = "text-block">spots that can be used to esti... | ["[Generation of agarose coated slides]\n1. Prepare 2% agarose in ddH2O in small beaker and melt, e.g. in a microwave.2. Preheat microscopy slide to 70°C on a hot plate.3. Distribute ca. 350-400 μl of liquid agarose over the slide until it is covered.4. Let the agarose dry for at least 30 min.\nAt the end of this secti... |
51,756 | An X-HTDC method for estimating particulate phosphorus from microalgae | 6 | null | https://www.protocols.io/view/an-x-htdc-method-for-estimating-particulate-phosph-bwskpecw | Yingyu Hu, Zoe V. Finkel | TITLE: An X-HTDC method for estimating particulate phosphorus from microalgae
AUTHORS: Yingyu Hu, Zoe V. Finkel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here we describe a protocol to estimate particulate phosphorus associated with microalgae using an extra-high temperature dry combustion met... | ["[Sampling]\nSampling microalgae for total particulate phosphorus (i.e. intracellular phosphorus and adsorbed phosphorus)", "[Sampling]\nFilter microalgae in liquid media onto polycarbonate filters, using gentle vacuum pressure (5 inches Hg).", "[Sampling]\nRinse samples with filtered seawater", "[Sampling]\nPlace sam... |
31,721 | Preparation of Adult Rat Ventricular Myocytes for FRET Imaging Experiments | 1 | dx.doi.org/10.17504/protocols.io.ba8hiht6 | https://www.protocols.io/view/preparation-of-adult-rat-ventricular-myocytes-for-ba8hiht6 | Robert Harvey, Shailesh Agarwal | TITLE: Preparation of Adult Rat Ventricular Myocytes for FRET Imaging Experiments
AUTHORS: Robert Harvey, Shailesh Agarwal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol describing method for isolating rat cardiac myocytes for use in FRET-based imaging experiments.</div></div>
[... | ["Anesthetize adult Sprague-Dawley rat (250-300 mg) with intraperitoneal injection of pentobarbital (150 mg/kg).", "After the animal has entered a deep plane of anesthesia (loss of corneal and toe pinch reflexes), remove the skin over the chest area. Open up the chest cavity using scissors to cut along both sides of th... |
40,863 | Quality Assurance and Quality Control (Part 10 of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj57kq9n | https://www.protocols.io/view/quality-assurance-and-quality-control-part-10-of-p-bj57kq9n | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Quality Assurance and Quality Control (Part 10 of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is Part 10 of "Phase 3 randomized, double-blinded, placebo-controlled t... | [] |
41,530 | ELISA for quantification of Granulocyte-colony stimulator factor (G-CSF) in human serum or plasma | 6 | dx.doi.org/10.17504/protocols.io.bks2kwge | https://www.protocols.io/view/elisa-for-quantification-of-granulocyte-colony-st-bks2kwge | Angel Justiz-Vaillant, Belkis Ferrer-Cosme | TITLE: ELISA for quantification of Granulocyte-colony stimulator factor (G-CSF) in human serum or plasma
AUTHORS: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. An anti-human granulocyte-colony stimulator factor (G-CSF) coating antibody is adsorbed onto t... | ["An anti-human granulocyte-colony stimulator factor (G-CSF) coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.", "Add 50 µl of human serum. Human G-CSF present in the serum sample binds to antibodies adsorbed into the microwells.", "The microplate is bloc... |
20,065 | U Mass - Cholesterol (LDL) | null | dx.doi.org/10.17504/protocols.io.xt9fnr6 | null | Jason Kim | TITLE: U Mass - Cholesterol (LDL)
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels... | ["Perform daily quality control assessment of instrumentation before analysis.", "Load each sample into a specialized micro-sample cup for the clinical chemistry analyzer.", "Select Cholesterol (LDL) test on display and run the analysis.", "Collect and analyze the data."] |
99,978 | Stereotaxic Injection by Iontophoresis | 1 | dx.doi.org/10.17504/protocols.io.14egn8ewzg5d/v4 | https://www.protocols.io/view/stereotaxic-injection-by-iontophoresis-ddvi264e | Allen Institute for Brain Science | TITLE: Stereotaxic Injection by Iontophoresis
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
This protocol describes the delivery of a neuronal tracer using the iontophoretic method. The surgery uses a stereotaxic system to target specific brain coordinates in the mouse.
Note: Research reported in this pu... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gkibuue | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Create a script to add functional information about the samples into Anvi'o. </p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
81,697 | 6.Taxon Group: Colonial Ascidiacea | 4 | dx.doi.org/10.17504/protocols.io.j8nlkwxq5l5r/v1 | https://www.protocols.io/view/6-taxon-group-colonial-ascidiacea-ctz9wp96 | John Bishop, Inez Januszczak | TITLE: 6.Taxon Group: Colonial Ascidiacea
AUTHORS: John Bishop, Inez Januszczak
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedures (SOPs) for Marine Metazoa", lead by the Other Metazoa Working Group. The SOP collection contains guidance on how to process the various marine ... | [] |
47,324 | Planting Tobacco Seeds in the Greenhouse | 4 | null | https://www.protocols.io/view/planting-tobacco-seeds-in-the-greenhouse-bsf4nbqw | Lynn Doran | TITLE: Planting Tobacco Seeds in the Greenhouse
AUTHORS: Lynn Doran
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for planting, germinating, and growing tobacco seedlings in the UIUC RIPE greenhouse. Has been verified on Petite Havana and Samson cultivars. </div><div class = "text-block... | ["Fill an 8\" azalea pot completely full with Berger BM6 HP Myco Organic Fertilized Peat Moss Mix soil.", "Tamp the soil down firmly with your hands. Trying to make as level of a surface as possible.", "Using the super fine (0.5 GPM @ 40 PSI) fogg-IT drench nozzle, soak the soil until saturated to remove any air bubble... |
null | null | null | dx.doi.org/10.17504/protocols.io.dpj5km | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is a protocol from: <br /><br />Brussaard, C. P. D., J. P. Payet, C. Winter, and M. G. Weinbauer. 2010. Chapter 11: Quantification of aquatic viruses by flow cytometry. Manual of Aquatic Viral Ecology. Waco, TX:American Society of Limnology and Oceanography. doi:1... | [] |
31,743 | Whole Blood Processing (SepMate) | null | dx.doi.org/10.17504/protocols.io.ba87ihzn | null | Yang Sun, David Lee, Jimmie Ye | TITLE: Whole Blood Processing (SepMate)
AUTHORS: Yang Sun, David Lee, Jimmie Ye
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Purpose: To purify PBMCs from 20 or 50mL whole blood (per donor). SepMate tubes will be used with Lymphoprep to ease processing time and effort. </div></div>
[STEPS]
?. [P... | ["[Plasma Separation]\nPurify 20mL or 50mL whole blood using the following protocol:", "[Plasma Separation]\nCentrifuge blood samples at swinging-out rotor and brake off within of collection.\nCentrifuge: 1200 34, 10 min", "[Plasma Separation]\nCarefully transfer from each tube in a 15mL tube avoiding the buffy coat,... |
42,797 | Rotina de Manutenção e Infecção de Células C6/36_v2 | 4 | null | https://www.protocols.io/view/rotina-de-manuten-o-e-infec-o-de-c-lulas-c6-36-v2-bm2mk8c6 | Fabio Gomes | TITLE: Rotina de Manutenção e Infecção de Células C6/36_v2
AUTHORS: Fabio Gomes
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocolo de Manutenção de Infecção (DENV e ZIKV) de Células C6/36 - PLO</div></div>
[STEPS]
?. [Tripsinização Cultura]
Pre-aquecer o meio e PBS 1x
?. [Tripsinização Cult... | ["[Tripsinização Cultura]\nPre-aquecer o meio e PBS 1x", "[Tripsinização Cultura]\nDe uma garrafa (25cm2) com 90% de confluência, remover o meio e lavar com 5 mL PBS (2)", "[Tripsinização Cultura]\nAcrescentar 1 mL tripsina", "[Tripsinização Cultura]\nDeixar agir por 1 min e acompanhe em microscopio", "[Tripsinização C... |
22,579 | Euplotes crassus GFP-neo artificial nanochromosomes sequence | null | dx.doi.org/10.17504/protocols.io.2atgaen | null | Angela Piersanti, Rachele Cesaroni | TITLE: Euplotes crassus GFP-neo artificial nanochromosomes sequence
AUTHORS: Angela Piersanti, Rachele Cesaroni
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-style:italic;">Euplotes crassus </span><span>GFP-neo artificial nanochromosomes </span></div></div>
[STEPS]
?. CCCCAAAA... | ["CCCCAAAACCCCAAAACCCCAAAACCCCATTTATTCAAATTTTATTTGAAGTTTTATAAAAATATTTAAATCTAAAGATGAGAGAAATCGTACACGTTCAAGGAGGACAATGCGGAAACCAGATTGGTGCTAAGTTCTGGGAAGTCATCTCTGACGAACATGGTGTTGACCCAACTGGTACCTACCACGGAGACTCTGACTTGCAGCTCGAAAGAATCAACGTTTACTACAACGAAGCAACTGGCGGTAGATACGTGCCAAGAGCCGTCTTGATGGATCTCGAACCAGGAACCATGGACTCCGTCAGAGCCGGACCAT... |
59,602 | StyroStone: A protocol for scanning and extracting three-dimensional meshes of stone artefacts using Micro-CT scanners | 1 | dx.doi.org/10.17504/protocols.io.b6fsrbne | https://www.protocols.io/view/styrostone-a-protocol-for-scanning-and-extracting-b6fsrbne | Dominik Göldner, Fotios Alexandros Karakostis, Armando Falcucci | TITLE: StyroStone: A protocol for scanning and extracting three-dimensional meshes of stone artefacts using Micro-CT scanners
AUTHORS: Dominik Göldner, Fotios Alexandros Karakostis, Armando Falcucci
[DESCRIPTION]
This protocol presents the first detailed step-by-step pipeline for the 3D scanning and post processin... | ["[Part 1 - Styrofoam preparation] Note: Depending on the number of lithics that need to be scanned, this section might take several hours.\n\nSelect a blank Styrofoam body (or similar material) to hold the artefacts. It should be thick enough to carve holes on both sides (Face A and Face B).", "[Part 1 - Styrofoam pre... |
34,627 | Fitting of SAXS data using SCATTER | 1 | null | https://www.protocols.io/view/fitting-of-saxs-data-using-scatter-bd3bi8in | Chris Berndsen | TITLE: Fitting of SAXS data using SCATTER
AUTHORS: Chris Berndsen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Scatter is a Java-based Small Angle X-ray Scattering software developed by Rob Rambo. It can be used for processing and analyzing data to calculate Rg, Dmax, and other parameters. If th... | ["[Buffer subtraction]\nStart and select Subtract from the left side menu.", "[Buffer subtraction]\nDrag your .dat files collected on your sample into the top pane.", "[Buffer subtraction]\nDrag your .dat files collected on your buffer into the bottom pane.", "[Buffer subtraction]\nLook at the similarity vs. frame win... |
null | null | null | dx.doi.org/10.17504/protocols.io.cgztx5 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
37,588 | Guide RNA Library Titration of Cas9 Cell Lines | 1 | dx.doi.org/10.17504/protocols.io.bgxujxnw | https://www.protocols.io/view/guide-rna-library-titration-of-cas9-cell-lines-bgxujxnw | Verity Goodwin, Emily Souster, Charlotte Beaver, Adam Jackson, Rizwan Ansari, Mathew Garnett, Fiona Behan | TITLE: Guide RNA Library Titration of Cas9 Cell Lines
AUTHORS: Verity Goodwin, Emily Souster, Charlotte Beaver, Adam Jackson, Rizwan Ansari, Mathew Garnett, Fiona Behan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for the gRNA library titration of cas9 expressing cancer cell line... | ["[Day 1]\nDetach and collect cas9 cells as per protocol \"Passaging adherent cancer cell lines\" steps 1-8 found here: https://protocols.io/view/passaging-adherent-cancer-cell-lines-bgtbjwin.html", "[Day 1]\nPrepare a cell suspension containing 1.25x106 cells in complete media to make a final concentration of 1.56x10... |
64,752 | Workflow for human fallopian tube and uterine endomyometrium bulk ATACseq | 4 | dx.doi.org/10.17504/protocols.io.5jyl898brv2w/v1 | https://www.protocols.io/view/workflow-for-human-fallopian-tube-and-uterine-endo-cbgqsjvw | Scott Lindsay-Hewett, Valentina Stanley, Mana Parast, Louise Laurent | TITLE: Workflow for human fallopian tube and uterine endomyometrium bulk ATACseq
AUTHORS: Scott Lindsay-Hewett, Valentina Stanley, Mana Parast, Louise Laurent
[DESCRIPTION]
Described here is the workflow used by the Female Reproductive Tissue Mapping Center at UCSD to generate bulk ATACseq data from human fallopi... | ["[Tissue preparation] As soon as possible after sterilization (salpingectomy or tubal ligation), prepare fallopian tube tissue according to the following protocol:\n\nHuman Pregnant Fallopian Tube Tissue Collection and Preservation Methods - UCSD Female Reproductive TMC\n\nAt the time of C-section, prepare uterine end... |
79,330 | Effects of hypolipemic drugs on psoriasis | 1 | dx.doi.org/10.17504/protocols.io.261ge311wl47/v1 | https://www.protocols.io/view/effects-of-hypolipemic-drugs-on-psoriasis-crqav5se | Mateusz Matwiejuk, Hanna Mysliwiec, Olivia Jakubowicz-Zalewska, Adrian Chabowski, Iwona Flisiak | TITLE: Effects of hypolipemic drugs on psoriasis
AUTHORS: Mateusz Matwiejuk, Hanna Mysliwiec, Olivia Jakubowicz-Zalewska, Adrian Chabowski, Iwona Flisiak
[DESCRIPTION]
Psoriasis is a chronic, systematic, inflammatory disease in which, multiple metabolic and immunologic disturbances lead to lipid abnormalities, impaire... | [] |
47,948 | protocol_CPG | 3 | dx.doi.org/10.17504/protocols.io.bs3kngkw | https://www.protocols.io/view/protocol-cpg-bs3kngkw | juanruanoruiz , fjgg79 , Pedro J Gómez Arias, Jesús Gay-Mimbrera, Macarena Aguilar-Luque, Salvador Peiró Moreno, Beatriz Isla-Tejera | TITLE: protocol_CPG
AUTHORS: juanruanoruiz , fjgg79 , Pedro J Gómez Arias, Jesús Gay-Mimbrera, Macarena Aguilar-Luque, Salvador Peiró Moreno, Beatriz Isla-Tejera
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Hypothesis</span></div><div class = "text-block">Th... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.qhbdt2n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Dinoflagellates are unicellular algae that can have photosynthetic or nonphotosynthetic lifestyles. Dinoflagellates in the genus <em>Symbiodinium</em> can enter endosymbiotic associations with corals, providing the metabolic basis for the highly productive and biologically di... | [] |
66,337 | Testo Edge X: Reviews Benefits,-that boosts testosterone levels, increases lean muscle mass | 3 | dx.doi.org/10.17504/protocols.io.dm6gpbkb8lzp/v1 | https://www.protocols.io/view/testo-edge-x-reviews-benefits-that-boosts-testoste-ccz9sx96 | leesopers | TITLE: Testo Edge X: Reviews Benefits,-that boosts testosterone levels, increases lean muscle mass
AUTHORS: leesopers
[DESCRIPTION]
TestoEdgeX
[STEPS] | [] |
61,558 | Getting started with Micro-Meta App Tutorial | 5 | null | https://www.protocols.io/view/getting-started-with-micro-meta-app-tutorial-b8cwrsxe | Alessandro Rigano, Mathias Hammer, Joel Ryan, Claire M. Brown, David Grunwald, Caterina Strambio De Castillia | TITLE: Getting started with Micro-Meta App Tutorial
AUTHORS: Alessandro Rigano, Mathias Hammer, Joel Ryan, Claire M. Brown, David Grunwald, Caterina Strambio De Castillia
[DESCRIPTION]
For quality, interpretation, reproducibility, and sharing value, microscopy images should be accompanied by detailed descripti... | ["[Before the tutorial] Video introduction", "[Tutorial - 1 - Download and Install Micro-Meta App] Download Micro-Meta App\n\nFollow the instructions in this step and in Video 3 of the tutorial series to download and install the stand-alone version of the Micro-Meta App.", "[Tutorial - 1 - Download and Install Micro-Me... |
null | null | null | dx.doi.org/10.17504/protocols.io.krmcv46 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Concentrate phytoplankton samples about 100-fold typically from 5L down to 20 mL. Takes about 1 hour per sample. Samples can be used for flow cytometry sorting or for cultures. Enrichment by TFF usually keep growing for a longer time than unconcentrate sam... | [] |
41,485 | Chlamydomonas reinhardtii cell wall proteins recrystallization | 4 | null | https://www.protocols.io/view/chlamydomonas-reinhardtii-cell-wall-proteins-recry-bkrmkv46 | Joao Vitor Molino | TITLE: Chlamydomonas reinhardtii cell wall proteins recrystallization
AUTHORS: Joao Vitor Molino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocols describe the steps required for the recrystalization of cell wall proteins from </span><span style = "font-style:italic;">Chlamydomon... | ["[Before start]\nCheck the protocol \"Chlamydomonas reinhardtii cell wall extraction with perchlorate\"The amount of recrystalized material varies, but in some results I was able to obtain 1% of the inicial cell mass as cell wall material. Recrystalization can be performed by diafiltration of dialysis.", "[By Diafiltr... |
17,877 | Tracing long-term demographic changes: The issue of spatial scales | null | dx.doi.org/10.17504/protocols.io.vpve5n6 | null | Johannes Mueller, Aleksandr Diachenko | TITLE: Tracing long-term demographic changes: The issue of spatial scales
AUTHORS: Johannes Mueller, Aleksandr Diachenko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The protocol concerns estimations of the population density in prehistory at different spatial scales. The following issues are add... | ["The coefficient representing the ‘weight’ of regions was estimated one time for each of regions, while the number of times a region was considered in different chronological periods is not taken into account. The relative sizes of the regions, as parts of the constant territory, are obtained as the result of the divi... |
86,483 | ADAPTATION OF HUMAN HEPATOCELLULAR CARCINOMA (HUH7) CELLS MAINTAINED IN HIGH-GLUCOSE DULBECCO’S MODIFIED EAGLE MEDIUM (DMEM) TO ROSWELL PARK MEMORIAL INSTITUTE (RPMI)-1640 MEDIUM | 4 | dx.doi.org/10.17504/protocols.io.n92ldmm97l5b/v1 | https://www.protocols.io/view/adaptation-of-human-hepatocellular-carcinoma-huh7-cyptxvnn | Rafidah Lani, Sazaly AbuBakar, pouya Hassandarvish | TITLE: ADAPTATION OF HUMAN HEPATOCELLULAR CARCINOMA (HUH7) CELLS MAINTAINED IN HIGH-GLUCOSE DULBECCO’S MODIFIED EAGLE MEDIUM (DMEM) TO ROSWELL PARK MEMORIAL INSTITUTE (RPMI)-1640 MEDIUM
AUTHORS: Rafidah Lani, Sazaly AbuBakar, pouya Hassandarvish
[DESCRIPTION]
This is a standard operating procedure (SOP) for thawing, c... | ["[Thawing Huh7 cells] Remove the cryovial of Huh7 cells from liquid nitrogen storage and quickly place it into a 37°C water bath. Gently shake the vial to hasten thawing.", "[Thawing Huh7 cells] Once thawed (within 1-2 minutes), clean the outside of the cryovial with 70% ethanol.", "[Thawing Huh7 cells] Transfer the t... |
62,567 | Extraction of Total Nucleic Acid from Wastewater Using the Promega Wizard Enviro Total Nucleic Acid Kit | 5 | dx.doi.org/10.17504/protocols.io.4r3l2oebxv1y/v1 | https://www.protocols.io/view/extraction-of-total-nucleic-acid-from-wastewater-u-b9cfr2tn | Tamara Walsky, Padmini Ramachandran, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: Extraction of Total Nucleic Acid from Wastewater Using the Promega Wizard Enviro Total Nucleic Acid Kit
AUTHORS: Tamara Walsky, Padmini Ramachandran, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
Wastewater based epidemiology has proven to be a useful tool in the COVID-19 pandemic, allowing pr... | ["[Capture and Concentration] Dispense 40 mL of wastewater or pasteurized wastewater into a", "[Capture and Concentration] Add 500 µL of Protease Solution. Mix well by inversion and incubate for 30 min at Room temperature .", "[Capture and Concentration] 3000 x g, 10 min, 25 Room temperature .", "[Capture and Concen... |
94,166 | Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment | 4 | dx.doi.org/10.17504/protocols.io.36wgq3zmylk5/v1 | https://www.protocols.io/view/lipopolysaccharide-intraperitoneal-injection-in-ra-c77wzrpe | mariangela.massarocenere | TITLE: Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment
AUTHORS: mariangela.massarocenere
[DESCRIPTION]
This protocol is a quick guide to how to prepare a solution of LPS on the saline vehicle, how to inject a rat for an intraperitoneal injection, and how to monitor the animal aft... | ["[1. Lipopolysaccharide (LPS) preparation] Before starting, it is better to prepare LPS solution on the same day it will be injected. However, under minimal conditions (that could be acceptable), you can work with LPS from previous days stored at kept at 2-8°C; in this case, shake very well before use.", "[1. Lipopoly... |
85,723 | EdU Staining Protocol in juvenile F. hepatica | 4 | dx.doi.org/10.17504/protocols.io.eq2lyjnrrlx9/v1 | https://www.protocols.io/view/edu-staining-protocol-in-juvenile-f-hepatica-cxx3xpqn | Paul McCusker, Duncan Wells, Rebecca Armstrong, Emily Robb, Paul McVeigh, Nathan Clarke, Erica Gardiner, Erin McCammick, Aaron Maule | TITLE: EdU Staining Protocol in juvenile F. hepatica
AUTHORS: Paul McCusker, Duncan Wells, Rebecca Armstrong, Emily Robb, Paul McVeigh, Nathan Clarke, Erica Gardiner, Erin McCammick, Aaron Maule
[DESCRIPTION]
This protocol describes the process of labelling and whole mount staining proliferative cells in in vitro juve... | ["[In vitro addition of EdU to juvenile F. hepatica] Juvenile liver fluke are cultured in 50% Chicken serum (CS50) as described in McCusker et al. (2016).", "10 µL of 10 millimolar (mM) 5-Ethynyl-2'-deoxyuridine (EdU) is mixed with 190 µL pre-warmed CS50 and added to worms for between 6 and 24 h.", "[Wash out EdU and c... |
82,323 | 10. Taxon Groups: Medusozoa and Ctenophora | 4 | dx.doi.org/10.17504/protocols.io.eq2ly7ooplx9/v1 | https://www.protocols.io/view/10-taxon-groups-medusozoa-and-ctenophora-cumtwu6n | Kesella Scott-Somme, Inez Januszczak | TITLE: 10. Taxon Groups: Medusozoa and Ctenophora
AUTHORS: Kesella Scott-Somme, Inez Januszczak
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedures (SOPs) for Marine Metazoa", lead by the Other Metazoa Working Group. The SOP collection contains guidance on how to process the... | [] |
66,321 | Simpli Acv Keto Gummies Reviews | Simpli Acv Keto Gummies Shark Tank | Simpli Acv Keto Gummies Reviews for Weight Loss | 3 | dx.doi.org/10.17504/protocols.io.ewov1nw42gr2/v1 | https://www.protocols.io/view/simpli-acv-keto-gummies-reviews-simpli-acv-keto-gu-cczrsx56 | christinealez | TITLE: Simpli Acv Keto Gummies Reviews | Simpli Acv Keto Gummies Shark Tank | Simpli Acv Keto Gummies Reviews for Weight Loss
AUTHORS: christinealez
[DESCRIPTION]
The main ingredient in Simpli Acv Keto Gummies is acai berry extract, which has been proven to help in weight loss by suppressing appetite and preventing ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ea8bahw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is part of the VERVE holiday drive to collect exotic off-the-shelf laboratory recipes. More details <a href="https://www.protocols.io/g/verve-net/news/call-for-recipes" target="_blank">here</a>.
[GUIDELINES]
<img id="s-mce-img" class="s-mce-img" src="https://s3.am... | [] |
85,825 | How to Cook Ramen | 1 | null | https://www.protocols.io/view/how-to-cook-ramen-cx29xqh6 | wenchaoh | TITLE: How to Cook Ramen
AUTHORS: wenchaoh
[DESCRIPTION]
The complete guide to making delicious ramen.
[STEPS]
SECTION: Supplies You Will Need
1. Cooking Pot
One Package of Instant Ramen
Chopsticks/Fork
Egg
Any Frozen Veggies (Corn, Peas, etc)
SECTION: The Noodles
2. Pour 2.5 cups of water into a cooking pot and... | ["[Supplies You Will Need] Cooking Pot\nOne Package of Instant Ramen\nChopsticks/Fork\nEgg\nAny Frozen Veggies (Corn, Peas, etc)", "[The Noodles] Pour 2.5 cups of water into a cooking pot and put on a high-heat stove. Wait until the water boils.", "[The Noodles] After the water starts boiling, add the Ramen and Sauce p... |
77,446 | Custom open-chamber microfluidic fabrication | 1 | dx.doi.org/10.17504/protocols.io.6qpvr47q2gmk/v1 | https://www.protocols.io/view/custom-open-chamber-microfluidic-fabrication-cpvevn3e | Quyen Do, Jimena Baleriola, Nora Bengoa-Vergniory, Richard Wade-Martins | TITLE: Custom open-chamber microfluidic fabrication
AUTHORS: Quyen Do, Jimena Baleriola, Nora Bengoa-Vergniory, Richard Wade-Martins
[DESCRIPTION]
This protocol described the fabrication of two- and three-open chambered microfluidics suitable for cell culturing using commercially acquired master mould.
[BEFORE_START... | ["[Casting of microfluidic devices] A custom master mould containing both duo- and trio-chambers (Figure 1) is manufactured by and obtained from Microliquid (Spain).", "[Casting of microfluidic devices] Mix 46 ml of silicon elastomer SylGARD 184, Dow Corning © with 4 mL of its curing agent (supplied in the same pack), ... |
84,020 | Mitochondrial isolation protocol | 4 | dx.doi.org/10.17504/protocols.io.kqdg3x4zzg25/v1 | https://www.protocols.io/view/mitochondrial-isolation-protocol-cwauxaew | Elias Adriaenssens | TITLE: Mitochondrial isolation protocol
AUTHORS: Elias Adriaenssens
[DESCRIPTION]
This protocol describes how to isolate crude mitochondrial fractions from HeLa cells.
[STEPS]
SECTION: Mitochondrial isolation
1. Seed HeLa cells in 15 cm dishes and grow until confluence. Treat the cells with DMSO or the mitophagy-ind... | ["[Mitochondrial isolation] Seed HeLa cells in 15 cm dishes and grow until confluence. Treat the cells with DMSO or the mitophagy-inducing cocktail Oligomycin/Antimycin A (O/A) if needed.", "[Mitochondrial isolation] Collect HeLa cells by trypsinization and resuspension in DMEM medium. Centrifuge the cells at 300 x g, ... |
63,659 | Testing the effect of E. coli cell supernatant and lysate on C. elegans behaviour | 4 | dx.doi.org/10.17504/protocols.io.n92ldzj27v5b/v1 | https://www.protocols.io/view/testing-the-effect-of-e-coli-cell-supernatant-and-caejsbcn | Saul Moore | TITLE: Testing the effect of E. coli cell supernatant and lysate on C. elegans behaviour
AUTHORS: Saul Moore
[DESCRIPTION]
Experiment to identify whether the behavioural effect on worms feeding on E. coli mutants from the initial screen of the Keio Collection are due to metabolite(s) present in either the cell supe... | ["[Cell culture on solid media] 25mL fresh LB broth was added to each of 2 Falcon tubes, and inoculated with BW25113 parent strain for the Keio Collection, and the E. coli gene-deletion mutant of interest, respectively.", "[Cell culture on solid media] The inoculations were left to grow overnight in a shaking incubator... |
null | null | null | dx.doi.org/10.17504/protocols.io.ekrbcv6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Purpose: To test axenic cultures for purity. <br />Protocol described in S. Bertillson, O. Berglund, D.M. Karl, S.W. Chisholm (2003). Elemental composition of marine Prochlorococcus and Synechococcus: Implications for the ecological stoichiometry of the sea. Limnol Oceanogr 4... | [] |
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