id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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87,901 | Evaluation of knowledge and awareness of pediatric oral health among school teachers of Hazaribag before and after oral health education: Study Protocol for Interventional study | 1 | dx.doi.org/10.17504/protocols.io.j8nlko926v5r/v1 | https://www.protocols.io/view/evaluation-of-knowledge-and-awareness-of-pediatric-cz35x8q6 | drvipinahuja, dr annapurna ahuja, dr nilima thosar | TITLE: Evaluation of knowledge and awareness of pediatric oral health among school teachers of Hazaribag before and after oral health education: Study Protocol for Interventional study
AUTHORS: drvipinahuja, dr annapurna ahuja, dr nilima thosar
[DESCRIPTION]
Background: The aim of this study will be assess the knowl... | ["[Introduction] Children’s growth and development is directly linked to their oral health. As they spend a\nlot of time in schools, oral education can bring an inevitable change in dental\nattitude and behaviour of these children, if taught with other subjects in\nschools. There is a paucity of studies reported in the... |
44,758 | PBMC- 05 - In Vitro Culture of TEFF+TREG - Cytokine Production by TEFF | 4 | dx.doi.org/10.17504/protocols.io.bpxwmppe | https://www.protocols.io/view/pbmc-05-in-vitro-culture-of-teff-treg-cytokine-pro-bpxwmppe | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: PBMC- 05 - In Vitro Culture of TEFF+TREG - Cytokine Production by TEFF
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">List of published work using this procedure:</... | ["Isolate TEFF and TREG with Miltenyi Kit according to the protocol PBMC- 03.", "Count both TEFF and TREG following the appropriate protocol (CELL COUNT- 02 or CELL COUNT- 03).", "Use sterile 96-well round bottom plates.\n[Consider that these plates can contain a volume of maximum 250µL]", "Centrifuge TEFF and TREG at ... |
null | null | null | dx.doi.org/10.17504/protocols.io.s3iegke | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Results of the additive and multiplicative diversity partitioning of gobiid fishes</strong></p>
[GUIDELINES]
<p>To obtain additive and multiplicative alpha and beta diversity, we used PARTITION 3; here can download manual and program: http://www.users.miamioh.edu/cri... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dqq5vv | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
58,029 | Measuring urea concentrations in water samples | 1 | dx.doi.org/10.17504/protocols.io.b4wmqxc6 | https://www.protocols.io/view/measuring-urea-concentrations-in-water-samples-b4wmqxc6 | Jacob Waldbauer, Amy Amy Zimmerman | TITLE: Measuring urea concentrations in water samples
AUTHORS: Jacob Waldbauer, Amy Amy Zimmerman
[DESCRIPTION]
Colorimetric assay for direct (vs. enzymatic) measurement of urea to a detection limit of 0.4 µM concentration. The reaction of urea with diacetylmonoxime (DAM) to form a colored product is enhanced by add... | ["Making Reagents and Solutions", "Diacetylmonoxime (DAM) solution: Dissolve 3.4 g in 100 mL nanopure or LC-MS water (34 g L-1 or 0.3363 M stock). Store solution at 4°C in dark. Stable for at least 1 month. \na. Also known as 2,3-butanedione monoxime.\nb. Dissolve using rotisserie (hybridization oven) set to room temp ... |
36,029 | Sample reception, unpacking and barcoding | null | dx.doi.org/10.17504/protocols.io.bfe5jjg6 | https://www.protocols.io/view/sample-reception-unpacking-and-barcoding-bfe5jjg6 | Leigh Jones, Philip Walker, Robert Goldstone, Simon Caidan | TITLE: Sample reception, unpacking and barcoding
AUTHORS: Leigh Jones, Philip Walker, Robert Goldstone, Simon Caidan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Purpose of examination / Clinical relevance</span></div><div class = "text-block"><div class = "justi... | ["Location of sample receipt and processing area: Clinical samples are delivered by courier and transferred to sample reception area by nominated staff.At the Receipt station, staff will act in the following capacities:Sample Checker (SC)Barcode Operator (BO)Barcode Label Operator (BLO)Sample Sorter (SS) Sample packagi... |
59,368 | idpr Workflow | 5 | dx.doi.org/10.17504/protocols.io.b58gq9tw | https://www.protocols.io/view/idpr-workflow-b58gq9tw | William McFadden, Judith Yanowitz | TITLE: idpr Workflow
AUTHORS: William McFadden, Judith Yanowitz
[DESCRIPTION]
This protocol details about idpr workflow.
[GUIDELINES]
References
Paper Citations
R / Package Citations
Additional Information:
Session Info
Runtime
[STEPS]
SECTION: Installing idpr: Downloading the Current Relea... | ["[Installing idpr: Downloading the Current Release] idpr is published in Bioconductor where the stable, released version of the package can be downloaded. The development version, which may be unstable, is published on GitHub.\nThe package can be installed from Bioconductor with the following line of code. This requir... |
91,515 | Data sharing barriers in a viral pandemic: semi-structured interview protocol | 1 | dx.doi.org/10.17504/protocols.io.n2bvj3j7xlk5/v1 | https://www.protocols.io/view/data-sharing-barriers-in-a-viral-pandemic-semi-str-c5k3y4yn | Yo Yehudi, Lukas Hughes-Noehrer, Carole Goble, Caroline Jay | TITLE: Data sharing barriers in a viral pandemic: semi-structured interview protocol
AUTHORS: Yo Yehudi, Lukas Hughes-Noehrer, Carole Goble, Caroline Jay
[DESCRIPTION]
In 2020, the COVID-19 pandemic resulted in a rapid response from governments and researchers worldwide. As of May 2022, over 6 million people died as a... | ["[Initial interview set-up] Start by preparing the materials you'll need for your interviews. \n\nIf interviews are in-person, prepare an audio and/or video recording setting which is quiet uninterrupted. \n\nIf interviews are online (e.g. via Zoom or other video conferencing tool), ensure that you have a quiet area t... |
40,773 | ELISA for quantification of IL-14 in human serum. | 6 | dx.doi.org/10.17504/protocols.io.bj3dkqi6 | https://www.protocols.io/view/elisa-for-quantification-of-il-14-in-human-serum-bj3dkqi6 | Angel Justiz-Vaillant | TITLE: ELISA for quantification of IL-14 in human serum.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. T... | ["An anti-human IL-14 coating antibody is adsorbed onto microwells by incubation overnight at 4°C.", "Add 50 µl of human serum. Human IL-14 present in the serum sample binds to antibodies adsorbed to the microwells.", "The microplate is blocked with 3% non-fat milk-PBS buffer and later wash to remove unbound proteins."... |
92,930 | Transfecting COS-1 cells using FuGENE® 4K Transfection Reagent and cell lysis (M-PER) in a 6-well plate | 1 | dx.doi.org/10.17504/protocols.io.261ged1rov47/v1 | https://www.protocols.io/view/transfecting-cos-1-cells-using-fugene-4k-transfect-c6zazf2e | Jailyn Izu | TITLE: Transfecting COS-1 cells using FuGENE® 4K Transfection Reagent and cell lysis (M-PER) in a 6-well plate
AUTHORS: Jailyn Izu
[DESCRIPTION]
General protocol for transfecting COS-1 cells with FuGene.
[STEPS]
SECTION: Lysis
9. Aspirate the media from all wells.
SECTION: Lysis
10. Wash each well 3 times with 2 mL i... | ["[Lysis] Aspirate the media from all wells.", "[Lysis] Wash each well 3 times with 2 mL ice cold 1X PBS (filtered).", "[Lysis] Add M-PER with 1X protease and phosphatase inhibitor to each well (see specific protocols for volume)\n\nBe sure to keep M-PER and samples on ice.\n\nNote: HALT protease and phosphatase inhibi... |
58,486 | ShortestSplitlineAlgorithm | 5 | null | https://www.protocols.io/view/shortestsplitlinealgorithm-b5cwq2xe | Jamal Thomas | TITLE: ShortestSplitlineAlgorithm
AUTHORS: Jamal Thomas
[DESCRIPTION]
Takes the US Census results and geographic data and creates electoral districts that are iso-populous (same population in each district). This eliminates gerrymandering since it only uses openly available census data, with an algorithm that can be ... | ["Place , , \"MakeFile\": \n \ninto an new folder. \nand \"run\":\n \ninto the folder labeled: \"State_Data\"", "Unzip the \"State_Data\" folder. (Warning: Be sure to only unzip the State_Data folder and nothing else as anything else will interfere with program execution)", "Compile the \"geoproc.cpp\" and \"block_... |
101,282 | BAF_Protocol_012_Lipidomics LC-MS(/MS): Vanquish UPLC and Orbitrap ID-X | 0 | dx.doi.org/10.17504/protocols.io.6qpvr885olmk/v1 | https://www.protocols.io/view/baf-protocol-012-lipidomics-lc-ms-ms-vanquish-uplc-de6a3hae | Nicholas Sherman | TITLE: BAF_Protocol_012_Lipidomics LC-MS(/MS): Vanquish UPLC and Orbitrap ID-X
AUTHORS: Nicholas Sherman
[DESCRIPTION]
This protocol is the basic LC and MS running parameters for lipids or very hydrophobic molecules. Most are singly charged. Lipids can be more challenging as more mass isoforms exist.
[GUIDELINES]
The... | ["[Prepare samples for injection] Add 10 uL of Splash Mix standard to the extracted lipids. Dry samples under liquid nitrogen at 40C.", "[Prepare samples for injection] Suspend dried extracted samples with 100 µL of 1:1 IPA/ACN", "[Prepare samples for injection] Mix well using a “figure-eight” movement of the vials in... |
13,136 | SPRI purification beads | null | dx.doi.org/10.17504/protocols.io.q3qdymw | null | Tomasz Suchan | TITLE: SPRI purification beads
AUTHORS: Tomasz Suchan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for preparing SPRI beads 100x cheaper than the commercial AMPure XP.</div><div class = "text-block">Based on DeAngelis et al. (1995) and online protocols:</div><div class = "text-block"><a ... | ["[Preparation of the solutions]\nPrepare 2.5 M solution of NaCl:place a 50 ml Falcon tube on the scale and tareadd 14.61 g of NaCl to the tubeadd water to 40 mldissolve NaClfill up the tube with water to 50 ml and mix", "[Preparation of the solutions]\nPrepare 50% solution of PEG-8000:place a 50 ml Falcon tube on the ... |
null | null | null | dx.doi.org/10.17504/protocols.io.pkudkww | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The data-base are monthly counts in five communal roosts of Blue-fronted Amazon (<em>Amazona aestiva</em>) in the Brazilian Pantanal, carried out from July, 2004 to June, 2009. The counts were stratified by flock-sizes.</p>
[STEPS] | [] |
62,522 | Nanopore (without barcode) | 4 | null | https://www.protocols.io/view/nanopore-without-barcode-b9a2r2ge | Hsin-Mao Wu | TITLE: Nanopore (without barcode)
AUTHORS: Hsin-Mao Wu
[DESCRIPTION]
Nanopore
[BEFORE_START]
1. 上機前先用QBIT測量DNA含量
2. 多樣本用Nanopore (with barcode) protocol
3. 依樣本數數據量決定是否加barcode
[GUIDELINES]
1. 含DNA禁止pipetting, vortex
2. 含DNA用輕彈、輕輕spin-down
3.使用filter tip
4. 多個樣本請放在水域槽或保冷孔盤
[STEPS]
SECTION: Step 1 End-prep.
30. 50 ... | ["[Step 1 End-prep.] 50 µL 中含有100 fmol DNA之DDW", "[Step 1 End-prep.] 上NEBiocalculator計算DNA用量\n並取出計算好用量的DNA", "[Step 1 End-prep.] ds:mass⇆moles", "[Step 1 End-prep.] moles→mass", "[Step 1 End-prep.] 輸入DNA片段長度及DNA fmol\n輸出DNA mass(ng)\nmass/conc.=DNA用量(μl)", "[Step 2 Adapter Ligation] 取Step 1之60 µL 產物", "[Step 1 End-pre... |
63,961 | Systematic review and meta-analysis of incidence and outcomes of hip and vertebral fractures hip fracture in patients with end-stage kidney disease | 1 | dx.doi.org/10.17504/protocols.io.3byl4brjrvo5/v3 | https://www.protocols.io/view/systematic-review-and-meta-analysis-of-incidence-a-capzsdp6 | Yoshinosuke Shimamura, Yasutaka Kuniyoshi, Hiroshi Ueta, Takamasa Miyauchi, Mari Yamamoto, Yasushi Tsujimoto | TITLE: Systematic review and meta-analysis of incidence and outcomes of hip and vertebral fractures hip fracture in patients with end-stage kidney disease
AUTHORS: Yoshinosuke Shimamura, Yasutaka Kuniyoshi, Hiroshi Ueta, Takamasa Miyauchi, Mari Yamamoto, Yasushi Tsujimoto
[DESCRIPTION]
This is the protocol for a ... | ["[Authors' Information] Last update: June 24 2022.\n\nAuthors: Yoshinosuke Shimamura MD, MPH1, 2; Yasutaka Kuniyoshi MD, PhD2,3; Hiroshi Ueta MD, PhD2,4; Takamasa Miyauchi MD, PhD2,5; Mari Yamamoto MD2,6; Yasushi Tsujimoto MD, MPH2, 7.\n\n1 Department of Nephrology, Teine Keijinkai Medical Center, Sapporo, Hokkai... |
94,869 | Standard DAB Staining for Free-floating Fixed NHP Brain Tissue | 4 | dx.doi.org/10.17504/protocols.io.261ged767v47/v2 | https://www.protocols.io/view/standard-dab-staining-for-free-floating-fixed-nhp-c8vvzw66 | Andreea C. Bostan | TITLE: Standard DAB Staining for Free-floating Fixed NHP Brain Tissue
AUTHORS: Andreea C. Bostan
[DESCRIPTION]
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.
This protocol has been te... | ["[Part I (Day 1)] Bring tissue to Room temperature in buffer (e g., Phosphate buffered saline, PBS) on an orbital shaker for 30 minutes. 30 min.", "[Part I (Day 1)] Prepare Peroxide Solution (0.3 - 3 % H2O2) in dH2O.\nE.g., for 10 mL 0.3% H2O2 use:\n100 µL 30% H2O2 \n9900 µL dH2O", "[Part I (Day 1)] Prepare Blocking S... |
52,143 | Detecting nitric oxide in free-living symbiotic dinoflagellates exposed to nanoparticles | 1 | dx.doi.org/10.17504/protocols.io.bw6pphdn | https://www.protocols.io/view/detecting-nitric-oxide-in-free-living-symbiotic-di-bw6pphdn | Liza M Roger, Nastassja Lewinski | TITLE: Detecting nitric oxide in free-living symbiotic dinoflagellates exposed to nanoparticles
AUTHORS: Liza M Roger, Nastassja Lewinski
[DESCRIPTION]
Diaminofuorescein-2 diacetate (DAF-2 DA) is a fluorescent indicator of nitric oxide (NO). The DAF reacts with the nitric anhydride (N2O3) which formed by oxidation of... | ["[Equipment] - 15 mL conical tubes\n- 50 mL conical tubes\n- 1.5 mL or 2 mL Eppendorf tubes\n- Multichannel pipettor (at least 8 positions) with 300μL volume/pipette\n- 1 mL pipettor and tips\n- 20-200 μL pipettor and tips\n- 10-100 μL pipettor and tips\n- 0.5 to 2.5 μL pipettor and tips\n- 96 well dosing plate, ro... |
28,416 | 18 Monitoring in living bacterial cells by UV-Vis spectroscopy | null | dx.doi.org/10.17504/protocols.io.7y8hpzw | null | TJUSLS China | TITLE: 18 Monitoring in living bacterial cells by UV-Vis spectroscopy
AUTHORS: TJUSLS China
[STEPS]
?. Pipet 5μL NDM-28a BL21(DE3) glycerol bacteria into 5ml LB medium, and 2.5μL kanamycin is added. Incubate aiming bacterial liquid at 37°C until its OD600 reach 0.5-0.6 then add inducer IPTG.
?. Centrifuge bacterial li... | ["Pipet 5μL NDM-28a BL21(DE3) glycerol bacteria into 5ml LB medium, and 2.5μL kanamycin is added. Incubate aiming bacterial liquid at 37°C until its OD600 reach 0.5-0.6 then add inducer IPTG.", "Centrifuge bacterial liquid and add phosphate buffer to resuspend bacterial precipitation, then centrifuge again and discard ... |
91,787 | Methodologies of chosen playlist selection for people living with dementia: A Systematic Review Protocol | 1 | dx.doi.org/10.17504/protocols.io.n2bvj3b9plk5/v1 | https://www.protocols.io/view/methodologies-of-chosen-playlist-selection-for-peo-c5vjy64n | bethanyroseevison, Nicolas Farina | TITLE: Methodologies of chosen playlist selection for people living with dementia: A Systematic Review Protocol
AUTHORS: bethanyroseevison, Nicolas Farina
[DESCRIPTION]
Abstract
Background: To further personalise and refine playlist collection methodologies for people with dementia, current utilised methodologies mus... | [] |
27,916 | MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing | null | dx.doi.org/10.17504/protocols.io.7hkhj4w | null | Hadas Keren Shaul, Hadas Keren-Shaul, Ephraim Kenigsberg, Diego Adhemar Jaitin, Eyal David, Franziska Paul, Amos Tanay, Ido Amit | TITLE: MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing
AUTHORS: Hadas Keren Shaul, Hadas Keren-Shaul, Ephraim Kenigsberg, Diego Adhemar Jaitin, Eyal David, Franziska Paul, Amos Tanay, Ido Amit
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-... | ["[Making MARS-seq2.0 384-well cell capture plates (Timing ~50 min for 24 plates)]\nTurn on the Bravo liquid-handling platform and wait for initialization to be completed. Load the ‘cell capture plate preparation’ protocol file for the 96ST head. The appropriate protocol file can be found in the Supplementary Software.... |
29,167 | ChroPlate - ProteinA | null | dx.doi.org/10.17504/protocols.io.8qphvvn | null | Alexandra Ehl, David Frommholz, Nadine Stefanczyk | TITLE: ChroPlate - ProteinA
AUTHORS: Alexandra Ehl, David Frommholz, Nadine Stefanczyk
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Purification Guide for the Isolation of Antibodies with ChroPlate Filtration Plates by DALEX Biotech.</span></div><div class = "tex... | ["[Load and Wash]\nEmpty the deep-well plate or place the ChroPlate on a clean one. Add 500 µl wash buffer to each well and centrifuge 2 minutes at 400 g in a swing-out rotor.\nIn case of unspecific hydrophobic and/or ionic interactions include up to 1 % Tween-20 and/or up to 0.5 M NaCl in the wash buffer.", "[Elution]... |
20,481 | Neural progenitor banking | null | dx.doi.org/10.17504/protocols.io.x89frz6 | null | Celeste Karch, Rita Martinez, Jacob Marsh | TITLE: Neural progenitor banking
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh
[STEPS]
?. Upon reaching at least 85% confluency, harvest neural progenitor cells as described in protocol below. Neural progenitor expansion protocol
?. Add equal volumes of NIM and 2x neural freezing medium to the NPC cell suspension... | ["Upon reaching at least 85% confluency, harvest neural progenitor cells as described in protocol below. Neural progenitor expansion protocol", "Add equal volumes of NIM and 2x neural freezing medium to the NPC cell suspension for a final 1 x 106 cells/mL.", "Gently mix solution and distribute into sterile cryovials. ... |
87,557 | Dementia post-diagnostic support in UK rural communities: experiences of people living with dementia, informal caregivers, and healthcare professionals. A systematic review protocol. | 1 | dx.doi.org/10.17504/protocols.io.81wgbxrdylpk/v1 | https://www.protocols.io/view/dementia-post-diagnostic-support-in-uk-rural-commu-czrdx526 | Dannielle Bilkey, Nicolas Farina, Ben Hicks | TITLE: Dementia post-diagnostic support in UK rural communities: experiences of people living with dementia, informal caregivers, and healthcare professionals. A systematic review protocol.
AUTHORS: Dannielle Bilkey, Nicolas Farina, Ben Hicks
[DESCRIPTION]
People living with dementia in rural areas experience numero... | [] |
93,740 | Tissue processing and freezing after surgery | 1 | null | https://www.protocols.io/view/tissue-processing-and-freezing-after-surgery-c7skzncw | Bettina Ergün | TITLE: Tissue processing and freezing after surgery
AUTHORS: Bettina Ergün
[DESCRIPTION]
The aim of this protocol is to document the processing of fresh tissue after surgery.
The collected tissue must also be documented in the files which are linked down below
The protocol descibes in detail how to process the res... | ["[Sample processing in the lab] Fresh tissue should be processed on the same day if possible, but can be stored at 4°C for a maximum of overnight.\nTo reduce pathogens, the tissue should be incubated for 60 min atRoom temperature", "[Sample processing in the lab]", "[Sample processing in the lab]", "[Sample processing... |
44,268 | DNA extraction protocol (Salting out) Modified | 3 | dx.doi.org/10.17504/protocols.io.bpgkmjuw | https://www.protocols.io/view/dna-extraction-protocol-salting-out-modified-bpgkmjuw | Afaq M.M. Niyas, Caterina Villari | TITLE: DNA extraction protocol (Salting out) Modified
AUTHORS: Afaq M.M. Niyas, Caterina Villari
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to extract DNA with modified salting out method.</div><div class = "text-block"><span>This protocol is part of the Bark Beetle ... | [] |
60,007 | Test for Nastia DO NOT APpROVE | 1 | dx.doi.org/10.17504/protocols.io.q26g74m41gwz/v1 | https://www.protocols.io/view/test-for-nastia-do-not-approve-b6ufretn | Avery Smith | TITLE: Test for Nastia DO NOT APpROVE
AUTHORS: Avery Smith
[DESCRIPTION]
abstract
[STEPS]
1. aaa
2 mL
2. bbbb
3. nnnn | ["aaa\n2 mL", "bbbb", "nnnn"] |
26,476 | Use of Health Improvement Card by Chinese physical therapy students: A pilot study | null | dx.doi.org/10.17504/protocols.io.54kg8uw | null | Alice Jones, XB Wu, YW Bai, Jia Han, Elizabeth Dean | TITLE: Use of Health Improvement Card by Chinese physical therapy students: A pilot study
AUTHORS: Alice Jones, XB Wu, YW Bai, Jia Han, Elizabeth Dean
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the methods for a study aimed to investigate perceptions of Chinese physical ... | ["Physical therapy students were recruited from two universities in Shanghai, China.", "Introduction of the Health Improvement Card (HIC) to physical therapy students from two universities in Shanghai via a standardized 45-min tutorial. Content of the tutorial follows the User Guide for administering the HIC; both of w... |
82,481 | 15. Taxon Group: Unitary Ascidiacea | 4 | dx.doi.org/10.17504/protocols.io.81wgbym6ovpk/v1 | https://www.protocols.click/view/15-taxon-group-unitary-ascidiacea-cusrwwd6 | John Bishop, Chris Fletcher, Inez Januszczak | TITLE: 15. Taxon Group: Unitary Ascidiacea
AUTHORS: John Bishop, Chris Fletcher, Inez Januszczak
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedures (SOPs) for Marine Metazoa", lead by the Other Metazoa Working Group. The SOP collection contains guidance on how to process th... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.k84czyw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>We attempt to link laboratory-based measures of preferences with measures of school performance. We measured in an incentivized way risk, time, social and competitive preferences and also cognitive abilities of university students and look for associations between these measu... | [] |
82,334 | Human brain section staining | 3 | dx.doi.org/10.17504/protocols.io.6qpvr4bb2gmk/v1 | https://www.protocols.io/view/human-brain-section-staining-cum6wu9e | Shiyi Wang | TITLE: Human brain section staining
AUTHORS: Shiyi Wang
[DESCRIPTION]
How to stain Human brain sections
[STEPS] | [] |
100,959 | DNA Extraction from Ethanol Zooplankton Samples - Phenol-Chloroform | 1 | dx.doi.org/10.17504/protocols.io.n92ld88mnv5b/v1 | https://www.protocols.io/view/dna-extraction-from-ethanol-zooplankton-samples-ph-det73ern | Andreas Novotny, Colleen Kellogg, rute.carvalho Carvalho, Matt Lemay | TITLE: DNA Extraction from Ethanol Zooplankton Samples - Phenol-Chloroform
AUTHORS: Andreas Novotny, Colleen Kellogg, rute.carvalho Carvalho, Matt Lemay
[DESCRIPTION]
This protocol extracts genomic DNA from bulk zooplankton samples, preserved either in ethanol or frozen - using Phenol/Chloroform extraction. The protoc... | ["[LYSIS AND INCUBATION] Add 100 µL lysozyme (125 mg fully dissolved in 1000 μl 1 x TE) and 20µL RNase A (10 µg/ml: 1µL in 999µL 1 x TE) to each sample tube.\nIncubate samples in a rotating incubator at 37°C for 1h.", "[LYSIS AND INCUBATION] Add 100 µL Proteinase K and 100 µL (20%) SDS to each lysate tube. \nIncubate a... |
87,308 | Sanger Tree of Life Fragmented DNA clean up: Manual SPRI | 4 | dx.doi.org/10.17504/protocols.io.kxygx3y1dg8j/v1 | https://www.protocols.io/view/sanger-tree-of-life-fragmented-dna-clean-up-manual-czhkx34w | Michelle Strickland, Clare Cornwell, Caroline Howard | TITLE: Sanger Tree of Life Fragmented DNA clean up: Manual SPRI
AUTHORS: Michelle Strickland, Clare Cornwell, Caroline Howard
[DESCRIPTION]
This protocol describes the manual clean up of fragmented DNA following the Sanger Tree of Life HMW DNA Fragmentation protocols, using PacBio AMPure PB beads. This process is high... | ["[Laboratory protocol] Using a standard pipette tip, measure the post-shearing sample volume and transfer DNA solution from Diagenode tube to a new labelled 1.5 mL microcentrifuge tube. Record the volume.", "[Laboratory protocol] Calculate the volume of AMPure PB beads needed for each sample based on the sheared DNA v... |
null | null | null | dx.doi.org/10.17504/protocols.io.p9kdr4w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="font-weight: 400;">Single-cell RNA sequencing (scRNA-seq) has emerged as a central genome-wide method to characterize cellular identities and processes. Consequently, improving its sensitivity, flexibility and cost-efficiency can advance many research questions. ... | [] |
20,996 | UC Davis - LDL Protocol | null | dx.doi.org/10.17504/protocols.io.yrcfv2w | null | Peter Havel | TITLE: UC Davis - LDL Protocol
AUTHORS: Peter Havel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">LDL and VLDL are separated from HDL using a precipitation reagent. Then the HDL fraction is measured for either TC or... | ["Add 25µl 2X precipitation buffer to 25µl of sample using a positive displacement pipet.", "Vortex and let sit at RT for 10 minutes.", "Centrifuge at 2000×g for 10 minutes at 4°C.", "Pipet supernatant into new tube, this is the HDL fraction.", "Add 5 µl of calibrator and sample to each well.IMPORTANT: Make sure not to... |
68,562 | Meat speciation NGS protocol using DNeasy mericon food kit, Ion AmpliSeq Library Kit Plus, Ion Chef, and Ion GeneStudio S5 System | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkxp7vmk/v1 | https://www.protocols.io/view/meat-speciation-ngs-protocol-using-dneasy-mericon-ce7sthne | Rebecca P Wilkes, Jobin J Kattoor | TITLE: Meat speciation NGS protocol using DNeasy mericon food kit, Ion AmpliSeq Library Kit Plus, Ion Chef, and Ion GeneStudio S5 System
AUTHORS: Rebecca P Wilkes, Jobin J Kattoor
[DESCRIPTION]
The purpose of this protocol is to provide the standard operating procedures for determining meat species in a food sample. ... | ["[Nucleic Acid Extraction- DNeasy mericon food kit (Qiagen)] See information provided in Guidelines and Warnings prior to starting the procedure.", "[Nucleic Acid Extraction] To do before starting:\nHomogenize 2 grams of food material\nAdd the appropriate amount of ethanol (96-100%) to Buffer AW2 before using the firs... |
76,122 | Growing freshwater sponges from gemmules in the laboratory | 4 | dx.doi.org/10.17504/protocols.io.x54v9dkj4g3e/v1 | https://www.protocols.io/view/growing-freshwater-sponges-from-gemmules-in-the-la-cnj2vcqe | Scott Nichols | TITLE: Growing freshwater sponges from gemmules in the laboratory
AUTHORS: Scott Nichols
[DESCRIPTION]
This is a basic protocol for growing freshwater sponges from gemmules in the laboratory. We specifically developed this protocol for working with Ephydatia muelleri, but have used it for other species as well. This p... | ["[Gemmule sterilization] Place a 40-70 µm cell strainer into a clean 10 cm Petri dish filled with cold, lake/spring water.", "[Gemmule sterilization] Cut off the end of a p1000 pipette tip with scissors to increase the size of the opening. Using the trimmed pipette tip, transfer the isolated gemmules into the cell str... |
27,683 | MojoSort™ Mouse CD11c Nanobeads Column Protocol | null | dx.doi.org/10.17504/protocols.io.7abhian | null | Sam Li | TITLE: MojoSort™ Mouse CD11c Nanobeads Column Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple ... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with... |
90,918 | Knowledge transfer interventions on cancer in Africa and Asia : a scoping review | 1 | dx.doi.org/10.17504/protocols.io.36wgq3e83lk5/v1 | https://www.protocols.io/view/knowledge-transfer-interventions-on-cancer-in-afri-c42eyybe | Julie Robin, clemence.schantz, Kadiatou KANTE, Aurélien DANCOISNE, Valery Ridde | TITLE: Knowledge transfer interventions on cancer in Africa and Asia : a scoping review
AUTHORS: Julie Robin, clemence.schantz, Kadiatou KANTE, Aurélien DANCOISNE, Valery Ridde
[DESCRIPTION]
Introduction: Africa and Asia face many challenges related to knowledge transfer in the field of cancer diagnosis, treatment, su... | ["[Introduction] In 2020, breast cancer was the most common cancer in the world, accounting for 11.7% of the 19.3 million cancers diagnosed worldwide. Its incidence continues to rise, particularly in southern countries, and equity in breast cancer is a major concern. Cancer control capacity is inadequate in many countr... |
24,840 | Barcode plasmid library cloning | null | dx.doi.org/10.17504/protocols.io.4hggt3w | null | Benjamin Emert | TITLE: Barcode plasmid library cloning
AUTHORS: Benjamin Emert
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for cloning Time Machine barcode plasmid library. </div></div>
[STEPS]
?. [Gibson assemnbly]
Purify Gibson reaction using PCR clean-up column according to manufacturer's protocol ... | ["[Gibson assemnbly]\nPurify Gibson reaction using PCR clean-up column according to manufacturer's protocol eluting in 20 microliters of", "Add 40 microliters of DNA binding buffer to 20 microliters of Gibson assembly.\n[DNA binding buffer]\n[Gibson assembly ]", "Add 200 microliters DNA wash buffer. Make sure ethanol h... |
66,339 | Via Keto Apple Gummies (Today Update 2022 Australia & Canada Price) Real Trusted Reviews! | 3 | dx.doi.org/10.17504/protocols.io.n2bvj6w6xlk5/v1 | https://www.protocols.io/view/via-keto-apple-gummies-today-update-2022-australia-cc2bsyan | Via Keto Apple Gummies | TITLE: Via Keto Apple Gummies (Today Update 2022 Australia & Canada Price) Real Trusted Reviews!
AUTHORS: Via Keto Apple Gummies
[DESCRIPTION]
Health
[STEPS] | [] |
48,812 | Calibrating Vaisala HMP45 | 1 | null | https://www.protocols.io/view/calibrating-vaisala-hmp45-btwknpcw | USDA | TITLE: Calibrating Vaisala HMP45
AUTHORS: USDA
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Calibrating Vaisala HMP45</div></div>
[STEPS]
?. Clean the sensorRemove the cap and filter. The filter is like Teflon tape, very fragile. Use DI water and a Q-tip to remove dirt/dust. The filter can usual... | ["Clean the sensorRemove the cap and filter. The filter is like Teflon tape, very fragile. Use DI water and a Q-tip to remove dirt/dust. The filter can usually be unscrewed from inside the cap, if it will not turn while using your finger, then a screwdriver to push it out from the top is a last resort. There is a possi... |
70,491 | SuperSoil - Soil DNA Extraction | 4 | dx.doi.org/10.17504/protocols.io.4r3l27864g1y/v1 | https://www.protocols.io/view/supersoil-soil-dna-extraction-cg33tyqn | Jie Hao Ou, Yin-Tse Huang | TITLE: SuperSoil - Soil DNA Extraction
AUTHORS: Jie Hao Ou, Yin-Tse Huang
[DESCRIPTION]
SuperSoil - Soil DNA Extraction
[GUIDELINES]
Keep all solutions at room temperature
[STEPS]
SECTION: Lysis
1. Add following materials to 1.8 ml centrifuge tube
250 mg soil
1.5 g Bead
800 µl SD1
SECTION: Lysis
2. Vortex for 10 ... | ["[Lysis] Add following materials to 1.8 ml centrifuge tube\n250 mg soil\n1.5 g Bead\n800 µl SD1", "[Lysis] Vortex for 10 min at max speed", "[Lysis] 14000 rpm, 1 min", "[Lysis] Transfer the supernatant to a clean 1.5 ml or 2 ml tube", "[Remove inhibitor] Add 200 ul SD2", "[Remove inhibitor] Vortex for 5 s at max spe... |
104,443 | Behavior monitoring of D. melanogaster using ethoscopes | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qyqjl1y/v1 | https://www.protocols.io/view/behavior-monitoring-of-d-melanogaster-using-ethosc-dh8339yn | Natalie Kaempf, Jorge S. Valadas, Antonio Ortega, Sha Liu, Patrik Verstreken | TITLE: Behavior monitoring of D. melanogaster using ethoscopes
AUTHORS: Natalie Kaempf, Jorge S. Valadas, Antonio Ortega, Sha Liu, Patrik Verstreken
[DESCRIPTION]
This protocol describes the practical steps to perform behavioral monitoring using ethoscopes. The cleaning and preparation of glass tubes as well as loadin... | ["2. day: take off the wax-food layer at the top, rinse tubes multiple times with water, wash tubes with 75% vinegar in demineralized water to remove scale from the glass tubes and incubate for 2-3h", "[Setup of ethoscopes] please follow publications: \nGeissmann, Quentin, Luis Garcia Rodriguez, Esteban J. Beckwith, Al... |
92,733 | Air Compressor Use and Maintenance | 4 | dx.doi.org/10.17504/protocols.io.81wgbxwm3lpk/v1 | https://www.protocols.io/view/air-compressor-use-and-maintenance-c6s5zeg6 | Ksenija Sabic | TITLE: Air Compressor Use and Maintenance
AUTHORS: Ksenija Sabic
[DESCRIPTION]
This manual includes instructions for regular use and maintenance of ACGP Series Air Compressor by Newport.
Instructions are taken directly from manufacturer website: https://www.newport.com/p/ACGP (also attached).
Please use the printed... | ["[Weekly Maintenance] Check for oil leaks, abnormal noise or vibration.", "[Monthly Maintenance] Check oil level.\n\nDrain the condensate that has collected in the air tank. It may be necessary to do more often when operated in high humidity.\n\nDrain the water collected in the air outlet filter.\n\nClean the compress... |
31,083 | Cystometry in awake rats | null | dx.doi.org/10.17504/protocols.io.bakjicun | https://www.protocols.io/view/cystometry-in-awake-rats-bakjicun | Janet Keast, Peregrine Osborne, Nicole Wiedmann | TITLE: Cystometry in awake rats
AUTHORS: Janet Keast, Peregrine Osborne, Nicole Wiedmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used for bladder cannulation and cystometry in an experimental adult male or female rat. The surgery is performed under anesthesia and should inco... | ["[Preparation for surgery]\nPrepare bladder cannula at least 24 - 48 h prior to surgery. This is comprised of a short length of PE tubing that connects directly with the bladder lumen; this tubing inserts into a long piece of PVC tubing that is exteriorised at the base of the neck. By carefully holding PE tubing over... |
85,795 | KAPP-Sen TMC: Dissociation of Pancreatic Acinar and Ducts (non-recovered) | 1 | dx.doi.org/10.17504/protocols.io.eq2lyjnorlx9/v1 | https://www.protocols.io/view/kapp-sen-tmc-dissociation-of-pancreatic-acinar-and-cx2bxqan | Juliana Alcoforado Diniz, Jessica Garofalo, Dylan Baker, Paul Robson | TITLE: KAPP-Sen TMC: Dissociation of Pancreatic Acinar and Ducts (non-recovered)
AUTHORS: Juliana Alcoforado Diniz, Jessica Garofalo, Dylan Baker, Paul Robson
[DESCRIPTION]
The dispersed samples were shipped cold from PRODOLABS. Prior to scRNA-seq, dispersed samples from brain dead donor’s pancreatic acinar and ducts ... | ["[Cell Dissociation with TrypLE]", "[Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling] Cells were fixated prior to scRNAseq according to https://dx.doi.org/10.17504/protocols.io.x54v9py5zg3e/v1", "[Cell Dissociation with TrypLE] Distribute specimens into 50 ml conical tubes, try to make similar cell con... |
null | null | null | dx.doi.org/10.17504/protocols.io.p4vdqw6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol decribes how to make 1 L of Luria-Bertani liquid medium (also known as LB broth) for the culturing of bacteria.</p>
[GUIDELINES]
<p>LB broth can be stored at room temperature once autoclaved and kept for up to 6 months.</p>
<p>If your broth becomes cloudy it ha... | [] |
66,877 | Beliv Support Healthy Blood Sugar [All Myth Busted] ] 10 You Thing About Now! | 3 | dx.doi.org/10.17504/protocols.io.8epv5981dg1b/v1 | https://www.protocols.io/view/beliv-support-healthy-blood-sugar-all-myth-busted-cdi5s4g6 | H H | TITLE: Beliv Support Healthy Blood Sugar [All Myth Busted] ] 10 You Thing About Now!
AUTHORS: H H
[DESCRIPTION]
Try not to stress regardless of whether you finish all containers of this enhancement and could do without the outcomes. You can reach them and request a total discount. It is just basic.
[STEPS] | [] |
48,186 | Streptococcal protein G and Protein-AG sandwich ELISA | 1 | dx.doi.org/10.17504/protocols.io.bta2nige | https://www.protocols.io/view/streptococcal-protein-g-and-protein-ag-sandwich-el-bta2nige | Angel Justiz-Vaillant | TITLE: Streptococcal protein G and Protein-AG sandwich ELISA
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA was used to study the interactions between Staphylococcal protein-G (SpG) and protein-AG (SpAG) with different immunoglobulin preparations from mamm... | ["This ELISA was used to study the interactions between Staphylococcal protein-G (SpG) and protein-AG (SpAG) with different immunoglobulin preparations from mammalian and avian species. The 96 well microtiter plate was coated overnight at 4°C with 2 µg/µl per well of SpG in carbonate-bicarbonate buffer pH 9.6.", "The p... |
63,910 | simply acv keto gummies reviews : Negative Reviews, Bad Complaints & Side Effects?Pills Advanced BHB Boost Ketogenic Supplement Exogenous Ketones for Men Women 60 Capsules 2 Bottles | 1 | dx.doi.org/10.17504/protocols.io.6qpvr62bbvmk/v1 | https://www.protocols.io/view/simply-acv-keto-gummies-reviews-negative-reviews-b-canesdbe | Simply Health ACV Keto Gummies | TITLE: simply acv keto gummies reviews : Negative Reviews, Bad Complaints & Side Effects?Pills Advanced BHB Boost Ketogenic Supplement Exogenous Ketones for Men Women 60 Capsules 2 Bottles
AUTHORS: Simply Health ACV Keto Gummies
[DESCRIPTION]
Simply Health ACV Keto Gummies
Simply Health ACV Keto Gummies, which are... | ["Simply Health ACV Keto Gummies : Negative Reviews, Bad Complaints & Side Effects?Pills Advanced BHB Boost Ketogenic Supplement Exogenous Ketones for Men Women 60 Capsules 2 Bottles", "Simply Health ACV Keto Gummies\nProduct Review: — Simply Health ACV Keto GummiesUsed For: — 🔶Weight Loss\n🔶Health Benefits\n🔶Burn ... |
null | null | null | dx.doi.org/10.17504/protocols.io.dy87zv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in "<a href="https://www.protocols.io/private/5098486f9db66cb9a8882b466a49d4fd" target="_blank">Fingerprinting aquatic virus communities using PFGE</a>"
[STEPS]
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gskbwcw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Denville LE Agarose is an all purpose agarose for routine nucleic acid electrophoresis of fragments between 500bp-23,000 bp.</p>
<p>Denville LE Agarose has no detectable DNase or RNase activity.</p>
<p> </p>
<p>Please refer to the appropriate protocol below, depending on whet... | [] |
43,309 | FCMPASS - Cataloguing fluorescence reference materials | 5 | dx.doi.org/10.17504/protocols.io.bnimmcc6 | https://www.protocols.io/view/fcmpass-cataloguing-fluorescence-reference-materia-bnimmcc6 | Joshua Welsh, Sean Cook, Jennifer Jones | TITLE: FCMPASS - Cataloguing fluorescence reference materials
AUTHORS: Joshua Welsh, Sean Cook, Jennifer Jones
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol outlines the steps required to catalogue fluorescence reference materials using the FCMPASS software. This is one of a number o... | ["[Opening the Bead Catalogue]\nOpen FCMPASS", "[Opening the Bead Catalogue]\nClick ‘Catalogue’ in the top menu bar", "[Bead Catalogue Fluorescence Overview]\nUnder the ‘Fluorescence’ tab entry fields exist for each of the pertinent metadata for reporting with fluorescence calibration.", "[Bead Catalogue Fluorescence O... |
96,306 | Ex vivo electrophysiology | 0 | dx.doi.org/10.17504/protocols.io.261gedqn7v47/v1 | https://www.protocols.io/view/ex-vivo-electrophysiology-daas2aee | Katerina Rademacher, Ken Nakamura | TITLE: Ex vivo electrophysiology
AUTHORS: Katerina Rademacher, Ken Nakamura
[DESCRIPTION]
This protocol describes steps for ex vivo electrophysiology in mouse brain slices. This protocol also includes instructions for clozapine N-oxide (CNO) testing in DREADD-expressing neurons.
[STEPS]
SECTION: Tissue Preparation
1.... | ["[Tissue Preparation] Deeply anesthetize the mouse with isofluorane, decapitate, and remove the brain.", "[Tissue Preparation] Using a vibratome, cut 150mm horizontal slices containing the region of interest in ice-cold aCSF solution and allow to recover at 33˚ C in aCSF for at least one hour.", "[Recording] For fluor... |
99,891 | Isolation of Nuclei from Human or NHP Brain Tissue | 1 | dx.doi.org/10.17504/protocols.io.ewov149p7vr2/v3 | https://www.protocols.io/view/isolation-of-nuclei-from-human-or-nhp-brain-tissue-ddst26en | Allen Institute for Brain Science | TITLE: Isolation of Nuclei from Human or NHP Brain Tissue
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis.
Note: Research reported in this publication was supported b... | [] |
86,414 | Protocol of a systematic review with metanalysis: forms of treatment in children diagnosed with congenital toxoplasmosis | 4 | dx.doi.org/10.17504/protocols.io.e6nvwddp2lmk/v1 | https://www.protocols.io/view/protocol-of-a-systematic-review-with-metanalysis-f-cymnxu5e | Sissi Kelly, José Roberto Mineo, Igor Mariano | TITLE: Protocol of a systematic review with metanalysis: forms of treatment in children diagnosed with congenital toxoplasmosis
AUTHORS: Sissi Kelly, José Roberto Mineo, Igor Mariano
[DESCRIPTION]
Toxoplasmosis is a zoonotic parasitic disease present
worldwide. Although the disease caused by T. gondii is usually
su... | ["[Background] Recently, congenital toxoplasmosis was also added to the list of diseases screened by the Piece test under the National Neonatal Screening Program [1]. In addition, gestational and congenital toxoplasmosis, in 2017, became a notifiable disease (mandatory communication to the health authority by doctors, ... |
21,290 | 18S Metagenomics in a Field Setting | null | dx.doi.org/10.17504/protocols.io.y2ifyce | null | Stefan Prost, Gideon Erkenswick, Mrinalini Watsa, Aaron Pomerantz | TITLE: 18S Metagenomics in a Field Setting
AUTHORS: Stefan Prost, Gideon Erkenswick, Mrinalini Watsa, Aaron Pomerantz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is was used to conduct DNA 18S metagenomics on FPI's Genomics in the Jungle - 2018 field course at the Green Lab, locate... | ["EquipmentBlueGel system MiniOne systemCreate .8 - 1.0% agarose 1 gel with 13 combs Measure 1 g of agaroseMix agraose with 100 mL of 1xTBEMicrowave the mixture until agarose is completely dissolved (1-3 min)Pour the agarose gel into the tray with the comb in place. Allow the agarose gel to harden (20-30 min)Create .8 ... |
55,771 | Making worm picks | 1 | dx.doi.org/10.17504/protocols.io.b2p3qdqn | https://www.protocols.io/view/making-worm-picks-b2p3qdqn | Bonnie Evans | TITLE: Making worm picks
AUTHORS: Bonnie Evans
[DESCRIPTION]
Making platinum wire picks for transferring C.elegans onto plates
[STEPS]
3. Light the ethanol burner. Take care that there is nothing directly above it, eg. papers attached to shelf.
1. Put on safety glasses.
4. Hold a disposable glass Pasteur pipette in ... | ["Light the ethanol burner. Take care that there is nothing directly above it, eg. papers attached to shelf.", "Put on safety glasses.", "Hold a disposable glass Pasteur pipette in one hand and the platinum wire with forceps in the other hand.", "Place the platinum wire inside the pipette and move it above the flame fo... |
78,956 | Neuromelanin staining (Fontana-Masson staining)-DAB staining on midbrain organoids | 1 | dx.doi.org/10.17504/protocols.io.kxygx98mdg8j/v1 | https://www.protocols.io/view/neuromelanin-staining-fontana-masson-staining-dab-crckv2uw | michela.deleidi, María José Pérez J., Hariam Raji, Pascale Baden, Federico Bertoli | TITLE: Neuromelanin staining (Fontana-Masson staining)-DAB staining on midbrain organoids
AUTHORS: michela.deleidi, María José Pérez J., Hariam Raji, Pascale Baden, Federico Bertoli
[DESCRIPTION]
Fontana-Masson staining is a silver staining technique that is commonly used to identify melanin-containing cells. That w... | ["[Neuromelanin staining] Incubate human midbrain organoid sections in a fresh solution of 3:1 methanol (MeOH)/3% hydrogen peroxide at Room temperature for 20 min.", "[Neuromelanin staining] Wash the slides and block it.", "[Neuromelanin staining] Apply primary antibodies in NGS 5% in PBS+ Triton-X 0.2% solution 60 min... |
null | null | null | dx.doi.org/10.17504/protocols.io.kdqcs5w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is used to prepare stock solutions for a M9-based ‘minimal’ medium containing trace metals, vitamins, and casamino acids. Pollen extract or other carbon sources can be added to promote growth of bee gut bacteria (proteobacteria).</p>
[STEPS]
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.pgmdju6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
48,739 | Plating Organoids | 4 | null | https://www.protocols.io/view/plating-organoids-btubnnsn | Morrisey Lab | TITLE: Plating Organoids
AUTHORS: Morrisey Lab
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Plating Organoids: Organoid Protocol Updated March 2020</div></div>
[STEPS] | [] |
91,110 | B-3 BLOOD STORAGE | 4 | dx.doi.org/10.17504/protocols.io.6qpvr3263vmk/v1 | https://www.protocols.io/view/b-3-blood-storage-c48eyzte | REDI-NET Consortium | TITLE: B-3 BLOOD STORAGE
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes blood storage.
[GUIDELINES]
OBJECTIVE
To outline steps for properly storing blood samples and nucleic acid samples purified from these samples.
SUMMARY/SCOPE
The overarching aim of the REDI-NET is to develop a collaborative... | ["[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Precool 96-well microfuge tube racks (for liquid forms including whole blood, plasma, serum, buffy coat) or sample box (for FTA cards only) on ice.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Using preprinted adhesive labels or permanent markers, label 1.5 ml microfuge tubes o... |
94,900 | Recombinant retroviral expression vectors that encode dominant-negative alleles of EGFR and ERBB2 | 1 | dx.doi.org/10.17504/protocols.io.bp2l6x5w1lqe/v1 | https://www.protocols.io/view/recombinant-retroviral-expression-vectors-that-enc-c8wuzxew | Ella Wilson, Markelle Scott, Vipasha Dwivedi, David J Riese II, Madison Zelan | TITLE: Recombinant retroviral expression vectors that encode dominant-negative alleles of EGFR and ERBB2
AUTHORS: Ella Wilson, Markelle Scott, Vipasha Dwivedi, David J Riese II, Madison Zelan
[DESCRIPTION]
EGFR and ERBB2 mutant alleles that encode proteins that lack tyrosine kinase activity typically possess a dominan... | ["[Introduction] We have described recombinant retroviral vectors based on pLXSN that encode a neomycin resistance gene and wild-type EGFR and ERBB2 alleles. Therefore, mammalian cells infected with these constructs are resistant to the antibiotic G418 [1].", "[Introduction] We have also described recombinant retrovir... |
null | null | null | dx.doi.org/10.17504/protocols.io.hfcb3iw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes the microarray-based quantification of human tRNAs. The tRNA microarray consists of 40 full-length tDNA probes recognising all 54 nuclearly encoded human isoacceptor tRNAs<sup>1</sup>. The array has been shown to reliably distinguish tRNA isoacceptors ... | [] |
67,437 | https://www.facebook.com/ViaKetoAppleGummiesinUnitedKingdom/ | 3 | dx.doi.org/10.17504/protocols.io.dm6gpbp8plzp/v1 | https://www.protocols.io/view/https-www-facebook-com-viaketoapplegummiesinunited-cd4ms8u6 | cdasfafaf | TITLE: https://www.facebook.com/ViaKetoAppleGummiesinUnitedKingdom/
AUTHORS: cdasfafaf
[DESCRIPTION]
https://www.facebook.com/ViaKetoAppleGummiesinUnitedKingdom/
[STEPS] | [] |
32,916 | Culture of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; f.2019-nCoV) | null | dx.doi.org/10.17504/protocols.io.bcduis6w | null | Alyssa Pyke, Ian M. Mackay, Frederick Moore, Andrew Van Den Hurk, Judy Northill, Mitchell Finger, Natalie Simpson, Neelima Nair, Peter Burtonclay, Peter Moore, Sarah Wheatley, Sean Moody, Sonja Hall-Mendelin, Elisabeth Gamez, Amanda De Jong, Ben Huang, Carmel Taylor, David Warrilow, Doris Genge, Glen Hewitson, Inga Sul... | TITLE: Culture of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; f.2019-nCoV)
AUTHORS: Alyssa Pyke, Ian M. Mackay, Frederick Moore, Andrew Van Den Hurk, Judy Northill, Mitchell Finger, Natalie Simpson, Neelima Nair, Peter Burtonclay, Peter Moore, Sarah Wheatley, Sean Moody, Sonja Hall-Mendelin, Elisab... | ["[Specimen and uninfected cell preparation]\nCell culture tubes were moved from the 37ºC incubator to a Class II Biosafety cabinet, within a PC3 laboratory environment.Growth medium from previously prepared Vero E6 tubes was discarded to waste.", "[Specimen and uninfected cell preparation]\nClinical samples were prep... |
null | null | null | dx.doi.org/10.17504/protocols.io.e7fbhjn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Procedure for making TCBS Agar plates for selection of Vibrio bacteria</p>
[STEPS]
?.
?.
?.
?. | [] |
38,969 | Preparation of soil bacteria for FCM | 4 | dx.doi.org/10.17504/protocols.io.biazkaf6 | https://www.protocols.io/view/preparation-of-soil-bacteria-for-fcm-biazkaf6 | Laura Espina | TITLE: Preparation of soil bacteria for FCM
AUTHORS: Laura Espina
[STEPS]
?. [Extraction of soil bacteria]
Measure the conductivity of the soil sample with a conductivimeter.
?. [Extraction of soil bacteria]
Prepare solution N: Add the needed amount of NaCl to 100 mL of distilled water to prepare a saline solution wit... | ["[Extraction of soil bacteria]\nMeasure the conductivity of the soil sample with a conductivimeter.", "[Extraction of soil bacteria]\nPrepare solution N: Add the needed amount of NaCl to 100 mL of distilled water to prepare a saline solution with the same conductivity that of the soil (see figure below). Autoclave.", ... |
99,989 | Nextera XT at 0.2X On the Mantis | 1 | dx.doi.org/10.17504/protocols.io.j8nlk57y1l5r/v3 | https://www.protocols.io/view/nextera-xt-at-0-2x-on-the-mantis-ddvv2666 | Allen Institute for Brain Science | TITLE: Nextera XT at 0.2X On the Mantis
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
Protocol to generate Nextera libraries using 0.2x reagents in a 96-well PCR plate using the Formulatrix Mantis instrument.
Note: Research reported in this publication was supported by the National Institute Of Mental Heal... | [] |
52,503 | FindingNemo in OneDay: Ultra-Long ONT Library Preparation from Cell to Flowcell in One Day | 1 | dx.doi.org/10.17504/protocols.io.bxhxpj7n | https://www.protocols.io/view/findingnemo-in-oneday-ultra-long-ont-library-prepa-bxhxpj7n | Inswasti Cahyani, John Tyson, Nadine Holmes, Josh Quick, Nicholas Loman, Matthew Loose | TITLE: FindingNemo in OneDay: Ultra-Long ONT Library Preparation from Cell to Flowcell in One Day
AUTHORS: Inswasti Cahyani, John Tyson, Nadine Holmes, Josh Quick, Nicholas Loman, Matthew Loose
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is focussed on the isolation of ultra-... | ["[Monarch UHMW DNA Extraction Kit]\nWe have obtained optimal DNA extractions using the NEB Monarch kit. This combines speed with high quality UHMW DNA. Follow the manufacturer’s instructions as described here, BUT incorporate the following changes as described below.\nOur most homogeneous extracted DNA samples were ob... |
null | null | null | dx.doi.org/10.17504/protocols.io.p2vdqe6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes how to make TRIS buffer (TRIS-HCl) at a 1M concentration and a pH 8.0</p>
[GUIDELINES]
<p>This protocol describes how to achieve a TRIS buffer at a pH 8.0. If a lower pH is required, i.e. pH 7.4, then add more hydrochloric acid until desired pH is rea... | [] |
47,218 | Cecret Workflow for SARS-CoV-2 Assembly and Lineage Classification | 5 | null | https://www.protocols.io/view/cecret-workflow-for-sars-cov-2-assembly-and-lineag-bscsnawe | Erin L Young, Technical Outreach and Assistance for States Team | TITLE: Cecret Workflow for SARS-CoV-2 Assembly and Lineage Classification
AUTHORS: Erin L Young, Technical Outreach and Assistance for States Team
[DESCRIPTION]
This protocol provides instructions to install and run the Cecret workflow as part of the StaPH-B Toolkit. Cecret produces SARS-CoV-2 consensus sequence ass... | ["[Software Dependencies] Load software dependencies\n \n\nWithin certain high-performance computing environments, these software can be loaded using GNU module commands similar to:", "[Installing StaPH-B Toolkit] The Cecret assembly workflow can be installed as part of the StaPH-B Toolkit using the following commands:... |
null | null | null | dx.doi.org/10.17504/protocols.io.hgpb3vn | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<ul>
<li>Make sure you are using fresh <em>E. coli</em> cells streaked for isolation on LB + antibiotics no more than 1 week from -80°C cryostock.</li>
</ul>
[STEPS]
?.
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46,991 | Neurolucida 360: Quick Surface tool for whole organ annotations | 1 | null | https://www.protocols.io/view/neurolucida-360-quick-surface-tool-for-whole-organ-br5pm85n | Maci Heal, sbaldwin | TITLE: Neurolucida 360: Quick Surface tool for whole organ annotations
AUTHORS: Maci Heal, sbaldwin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">How to create contours to map interior and exterior surfaces of intact organs in 3D microscopy images using the Quick Surface in Neurolucida 360.</div>... | ["Launch Neurolucida 360 with SPARC-mode enabled.", "[Quick Surface]\nIn the Trace ribbon, select Quick Surface. The Quick Surface window will appear.\nThe top portion of the window displays the image stack plane-by-plane. On the right side, there is a Z-plane slider and below that, a Z-position jump box.Tools and sett... |
68,930 | Fluorescent Immunolabelling for endogenous mouse LRRK2 | 4 | dx.doi.org/10.17504/protocols.io.261genqkog47/v1 | https://www.protocols.io/view/fluorescent-immunolabelling-for-endogenous-mouse-l-cfjatkie | Roberta Marongiu, andrew.west west | TITLE: Fluorescent Immunolabelling for endogenous mouse LRRK2
AUTHORS: Roberta Marongiu, andrew.west west
[DESCRIPTION]
This protocol is used to label endogenous LRRK2 in mouse.
It has been edited from West et al., 2014
Protocol optimized for fresh fixed brain tissue sectioned at 40 um.
[STEPS]
2. Wash 3 times for 10... | ["Wash 3 times for 10 min in TBS", "Sections were “quenched” in 100% Methanol for 15 min at 4 °C on shaker.", "Wash 3 times for 10 min in TBS", "Incubate the slices with Sodium Citrate – 10 mM Sodium Citrate pH 6.0 w/0.05% Tween for 30 min at 37 °C on shaker.", "Wash 3 times for 10 min in TBS", "During the washes, p... |
null | null | null | dx.doi.org/10.17504/protocols.io.mvdc626 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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53,546 | Coverslip Functionalization SOP003.v2.2 | 1 | null | https://www.protocols.io/view/coverslip-functionalization-sop003-v2-2-byiipuce | Rory Kruithoff, Douglas Shepherd | TITLE: Coverslip Functionalization SOP003.v2.2
AUTHORS: Rory Kruithoff, Douglas Shepherd
[DESCRIPTION]
Document Summary:
This document, SOP003 – Coverslip Functionalization, describes the process for cleaning and modifying the coverslip surface for improved adhesion of a sample.This procedure describes two methods o... | ["[Part 1 - Clean coverslips] Wash for 30 min via immersion in 1:1 mix of 37 % (v/v) and methanol at Room temperature.", "[Part 1 - Clean coverslips] Rinse 3x in DI H2O.", "[Part 1 - Clean coverslips] Fill beaker with Rnase-free water and autoclave coverslips to sterilize.", "[Part 1 - Clean coverslips] Rinse 1x in 70 ... |
76,700 | Run Clearmap 2 docker | 5 | null | https://www.protocols.io/view/run-clearmap-2-docker-cn54vg8w | Moritz Negwer | TITLE: Run Clearmap 2 docker
AUTHORS: Moritz Negwer
[DESCRIPTION]
This protocol is a supplement to our upcoming publication "FriendlyClearMap: An optimized toolkit for mouse brain mapping and analysis".
In this protocol, we describe in detail how to run Clearmap2's CellMap portion in a Docker container.
[STEPS]
SE... | ["[Docker Setup] On Windows: \nIf you haven't already, download and set up Docker Desktop for Windows. This requires administrator privileges and will also install the Windows Subsystem for Linux (WSL2). \n\nDownload: \nhttps://www.docker.com/products/docker-desktop \n\nAlternatively, use our install script via powersh... |
null | null | null | dx.doi.org/10.17504/protocols.io.rpxd5pn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>The architecture of normal and diseased tissues strongly influences the development and progression of disease as well as responsiveness and resistance to therapy. We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immuno-fluo... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.eagbabw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is based on Schleper et al. (1992) as modified by Stedman et al. (2003).<br /><br />This is a protocol from: <br /><br />Stedman, K. M., K. Porter, and M. L. Dyall-Smith. 2010. Chapter 6: The isolation of viruses infecting Archaea. Manual of Aquatic Viral Ecology. ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kebctan | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Biodiversity data exist in large quantities, which is a boon to biodiversity science. In spite of large numbers of data records being available, however, the proportion of those records that is readily usable for science applications is quite small. The difference between the... | [] |
81,145 | Protocol for highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming | 4 | dx.doi.org/10.17504/protocols.io.ewov1o6pylr2/v1 | https://www.protocols.io/view/protocol-for-highly-efficient-and-rapid-generation-ctgzwjx6 | Hongkui Deng | TITLE: Protocol for highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming
AUTHORS: Hongkui Deng
[DESCRIPTION]
We recently established a highly efficient and rapid chemical reprogramming system for generation of human pluripotent stem cells (hCiPSCs) (Liuyang et al., 2023). Her... | ["[Isolation and culture of hADSCs] Adult human adipose derived stromal cells (hADSCs) were isolated from donated adult adipose tissue that obtained with informed written consent. \nThe tissues (2-4 cm3) were washed twice with PBS containing 2% penicillin-streptomycin, minced as much as possible with scissors to 1-2 mm... |
null | null | null | dx.doi.org/10.17504/protocols.io.ds76hm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Separates molecules based on size. Great for checking DNA after a Restriction Digest. This protocol is for a 1.2% agarose gel, sufficient for resolving DNA 400bp to 7kb (conservatively). The gel electrophoresis system is a 7.5cm x 10cm gel bed area with approximately 15cm electr... | [] |
30,504 | Loop L1 (odd level) BsaI type IIS cloning into pCk vectors | null | dx.doi.org/10.17504/protocols.io.92gh8bw | null | Eftychis Frangedakis, Susana Sauret-Gueto, Anthony West, Nicola Patron, marta tomaselli, Marius Rebmann, Jim Haseloff | TITLE: Loop L1 (odd level) BsaI type IIS cloning into pCk vectors
AUTHORS: Eftychis Frangedakis, Susana Sauret-Gueto, Anthony West, Nicola Patron, marta tomaselli, Marius Rebmann, Jim Haseloff
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol based on:</div><div class = "text-block"><a href="... | ["[Loop pCk vectors]", "[Example of assembly of L0 parts into a transcription unit (L1)]", "[Protocol for assembly of L0 parts into a transcription unit (L1)]\nDetermine the concentrations of each DNA plasmid needed (L0 plasmids and pCk acceptor plasmid) by spectrophotometry (Nanodrop).In the example in step 2, determi... |
81,498 | Bead Beating in Custom Buffer Followed by XP Bead Cleanup (NGS Workflow) | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbz7rvx1/v1 | https://www.protocols.io/view/bead-beating-in-custom-buffer-followed-by-xp-bead-ctt2wnqe | Jason D Limberis, Alina Nalyvayko, Janré Steyn, Jennifer Williams, Melanie Grobbelaar, Robin M Warren, john.metcalfe | TITLE: Bead Beating in Custom Buffer Followed by XP Bead Cleanup (NGS Workflow)
AUTHORS: Jason D Limberis, Alina Nalyvayko, Janré Steyn, Jennifer Williams, Melanie Grobbelaar, Robin M Warren, john.metcalfe
[DESCRIPTION]
DNA extraction method for Mycobacterium tuberculosis from various sample types as described in: "... | ["[Prepare Buffers] ComponentAmount to addNaCl (5M)2 mLTris-HCl pH 8.3 (1M)1 mLEDTA pH 9.0 (0.5M)0.2 mLTriton X-1001 mLH2O95.8 mL\n \n\nComponentAmount for 100 mLTris-HCl 1M pH 81 mLEDTA 0.5M20 uL", "[Extract DNA] Transfer the inactivated bacterial suspension to a new well-labeled Starsted screw cap tube containing ~ 2... |
null | null | null | dx.doi.org/10.17504/protocols.io.mqec5te | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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49,282 | Tissue Nuclei Isolation and Glutaraldehyde Fixation for sci RNA-Seq | 1 | dx.doi.org/10.17504/protocols.io.budans2e | https://www.protocols.io/view/tissue-nuclei-isolation-and-glutaraldehyde-fixatio-budans2e | David Fraser Read, Cole Trapnell | TITLE: Tissue Nuclei Isolation and Glutaraldehyde Fixation for sci RNA-Seq
AUTHORS: David Fraser Read, Cole Trapnell
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details a method for isolating and fixing nuclei from primary tissue such that the resulting fixed nuclei are compatible ... | ["[Buffer Preparaion]\nNuclei Buffer: 10 mM Tris HCl, 10 mM NaCl, 3 mM MgCl2 in nuclease-free waterStore at 4C.", "[Dissociation and filtering]\nFor each sample to dissociate, add 5 mL of lysis/fixation buffer (out of 10 mL available per sample) to a Gentle MACS M Tube. Store both the M Tube and a second tube (holding ... |
42,632 | Benchmarking missing-values approaches for predictive models on health databases | 1 | null | https://www.protocols.io/view/benchmarking-missing-values-approaches-for-predict-bmvgk63w | Alexandre Perez-Lebel, Gaël Varoquaux, Marine Le Morvan, Julie Josse, Jean-Baptiste Poline | TITLE: Benchmarking missing-values approaches for predictive models on health databases
AUTHORS: Alexandre Perez-Lebel, Gaël Varoquaux, Marine Le Morvan, Julie Josse, Jean-Baptiste Poline
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">BACKGROUND</div><div class = "text-block">As databases grow larg... | ["[Introduction]\nThis protocol details the experiments run in the article Benchmarking missing-values approaches for predictive models on health databases, Perez-Lebel et al. 2021. Documented code is available on GitHub:And can be installed through the following steps:We benchmarked two sets of 9 supervised predictive... |
25,095 | RNA Isolation from Plant Tissue Protocol 6: pBIOZOL and Qiagen RNeasy Plant Mini Kit Method | null | dx.doi.org/10.17504/protocols.io.4rfgv3n | null | Marc T. J. Johnson, Eric J. Carpenter, Zhijian Tian, Richard Bruskiewich, Jason N. Burris, Charlotte T. Carrigan, Mark W. Chase, Neil D. Clarke, Sarah Covshoff, Claude W. dePamphilis, Patrick P. Edger, Falicia Goh, Sean Graham, Stephan Greiner, Julian M. Hibberd, Ingrid Jordon-Thaden, Toni M. Kutchan, James Leebens-Mac... | TITLE: RNA Isolation from Plant Tissue Protocol 6: pBIOZOL and Qiagen RNeasy Plant Mini Kit Method
AUTHORS: Marc T. J. Johnson, Eric J. Carpenter, Zhijian Tian, Richard Bruskiewich, Jason N. Burris, Charlotte T. Carrigan, Mark W. Chase, Neil D. Clarke, Sarah Covshoff, Claude W. dePamphilis, Patrick P. Edger, Falicia Go... | ["Grind tissue to a powder in liquid nitrogen.", "Add of cold () pBIOZOL reagent for up to of frozen ground tissue.\n1.3 ml\n4 °C\n100 mg", "Incubate the tube for at .\n0 Room temperature\nLay the tube down horizontally to maximize surface area during RNA extraction.", "Centrifuge for at in a microcentrifuge at .\n... |
31,076 | Immunohistochemical labeling of thick cryosections from pelvic ganglia | null | dx.doi.org/10.17504/protocols.io.bakcicsw | https://www.protocols.io/view/immunohistochemical-labeling-of-thick-cryosections-bakcicsw | Janet Keast, Peregrine Osborne | TITLE: Immunohistochemical labeling of thick cryosections from pelvic ganglia
AUTHORS: Janet Keast, Peregrine Osborne
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes immunohistochemical procedures applied to thick (50 µm) cryosections mounted directly on slides. It is used wh... | ["[Preparation of cryosections]\nCryoprotect fixed tissue in phosphate-buffered saline (PBS; 0.1 M, pH7.2) containing 30% sucrose. This should be performed at 4C, 24-72h prior to cutting.", "[Preparation of cryosections]\nEmbed tissue in cryomold using OCT, freeze in cryostat and cut sections (50 µm), distributing sect... |
54,417 | SNARE-seq2 | 1 | dx.doi.org/10.17504/protocols.io.bzdrp256 | https://www.protocols.io/view/snare-seq2-bzdrp256 | Nongluk Plongthongkum, Dinh H Diep, Song Chen, Blue Lake, Kun Zhang | TITLE: SNARE-seq2
AUTHORS: Nongluk Plongthongkum, Dinh H Diep, Song Chen, Blue Lake, Kun Zhang
[DESCRIPTION]
To study the heterogeneity of complex tissues by joint profiling of gene expression and its regulation, we require an accurate and high-throughput method. Here we described improved high-throughput combinato... | ["[Reagent setup] 40% (wt/vol) PEG 6000. Weigh 16.0 g of PEG 6000 in 50 mL tube. Add nuclease-free water and bring the total volume to 40 mL. Rotate the tube at room temperature until PEG 6000 completely dissolved. Spin down the tube at 200 g for 2 min, at room temperature to remove the tiny bubble. CRITICAL: 40% (wt/v... |
104,317 | Electrophysiology and 2-photon imaging of Ca+2-transients | 4 | dx.doi.org/10.17504/protocols.io.kxygx33yog8j/v2 | https://www.protocols.io/view/electrophysiology-and-2-photon-imaging-of-ca-2-tra-dh4538y6 | Beatriz E Nielsen, Andrew G Yee | TITLE: Electrophysiology and 2-photon imaging of Ca+2-transients
AUTHORS: Beatriz E Nielsen, Andrew G Yee
[DESCRIPTION]
This protocol describes the steps for imaging dendritic calcium transients evoked by backprogating action potentials. 2-photon imaging was performed using a 2-photon laser scanning microscopy system,... | ["[Acute brain slice preparation] Steps are described in the protocol linked below.", "[Rig setup] Turn on required devices and software for acquisition (Toronado and Axograph).\n\nToronado: https://github.com/StrowbridgeLab/Toronado-Laser-Scanning \nAxograph X (Axograph Scientific).", "[Rig setup] Place the intake lin... |
33,246 | Impact of a pre-feeding oral stimulation program on first feed attempt in preterm infants: double-blind controlled clinical trial | 1 | dx.doi.org/10.17504/protocols.io.bcp6ivre | https://www.protocols.io/view/impact-of-a-pre-feeding-oral-stimulation-program-o-bcp6ivre | Karine Da Rosa Pereira, Deborah Salle Levy, Renato S. Procianoy, Rita de Cássia Silveira | TITLE: Impact of a pre-feeding oral stimulation program on first feed attempt in preterm infants: double-blind controlled clinical trial
AUTHORS: Karine Da Rosa Pereira, Deborah Salle Levy, Renato S. Procianoy, Rita de Cássia Silveira
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Objective: To eva... | ["[PROCEDURES FOR SAMPLE SELECTION]\nPreterm infants were identified using medical record information. Following patient identification, parents or guardians were invited to participate in the study.", "[PROCEDURES FOR PATIENT RANDOMIZATION]\nThe randomization process was performed in the Random Alloc Software environm... |
null | null | null | dx.doi.org/10.17504/protocols.io.j7rcrm6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>In this protocol, we aimed to show the step-by.step procedures used to develop, evaluate and validate (CVD) risk charts.</p>
[STEPS]
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98,375 | Pseudoislets for diabetes research: Purifying and selective re-aggregating human islet cells | 4 | dx.doi.org/10.17504/protocols.io.5jyl85x7dl2w/v2 | https://www.protocols.io/view/pseudoislets-for-diabetes-research-purifying-and-s-dcbf2sjn | Wei Liu, Craig Dorrell, Xiaojuan Chen | TITLE: Pseudoislets for diabetes research: Purifying and selective re-aggregating human islet cells
AUTHORS: Wei Liu, Craig Dorrell, Xiaojuan Chen
[DESCRIPTION]
In vitro modeling of human islet cells for diabetes research utilizing purified and then selectively re-aggregated various combinations of human islet cells.
... | ["Human islet culture and single cell preparation\n\nIslets isolated from non-diabetic deceased human donors within 2-4 days post-isolation will be used in experiments. Islets and the dispersed cells are cultured at 37 °C with 5% CO² in a CMRL-1066 supplemented CIT medium (Cellgro, Cat. #98-304-CV) with 10 IU/ml Hepari... |
79,382 | Selection of Stationary Phase of HPLC for Posaconazole Estimation | 1 | dx.doi.org/10.17504/protocols.io.6qpvr4yn2gmk/v1 | https://www.protocols.io/view/selection-of-stationary-phase-of-hplc-for-posacona-crrwv57e | annamalai.rama | TITLE: Selection of Stationary Phase of HPLC for Posaconazole Estimation
AUTHORS: annamalai.rama
[DESCRIPTION]
Introduction: Posaconazole is a widely used antifungal drug, and its accurate quantification is essential for quality control and assessment of its pharmaceutical products. This study aimed to develop and val... | ["[Selection of Stationary Phase] To determine the appropriate stationary phase for the HPLC method, a total of 60 trials were conducted using various columns, including Phenomenex Luna C18, Phenomenex Kinetex C18, Phenomenex Hyperclone C18, and Agilent Eclipse XDB C18.", "[Selection of Stationary Phase] The selection ... |
103,698 | FruitRescue! - Apple phenotyping | 0 | null | https://www.protocols.io/view/fruitrescue-apple-phenotyping-dhhs336e | Mathieu Brisson, Amandine Cornille | TITLE: FruitRescue! - Apple phenotyping
AUTHORS: Mathieu Brisson, Amandine Cornille
[DESCRIPTION]
The FruitRescue project aims to create a pipeline to predict fruit trees' genomic offset. To confirm the pipeline predictions, tree fitness parameters are measured in crop and wild tree orchards along a longitudinal gradi... | ["[Trunk diameter] The trunk diameter is an indicator of the tree vigor (Waring 1987). Genetic information as well as environmental cues including abiotic and biotic stresses can modify the source-sink relationships and therefore modify the trunk growth during the year. Tree adaptation to its location can thus be asses... |
102,769 | JAX-Sen: Mouse pancreas dissociation for single-cell RNA sequencing | 0 | dx.doi.org/10.17504/protocols.io.ewov19zzylr2/v1 | https://www.protocols.io/view/jax-sen-mouse-pancreas-dissociation-for-single-cel-dgkr3uv6 | Ramalakshmi Ramasamy, Juliana Alcoforado Diniz, Patrick Fleming, Jessica Garofalo, Dylan Baker, Paul Robson | TITLE: JAX-Sen: Mouse pancreas dissociation for single-cell RNA sequencing
AUTHORS: Ramalakshmi Ramasamy, Juliana Alcoforado Diniz, Patrick Fleming, Jessica Garofalo, Dylan Baker, Paul Robson
[DESCRIPTION]
These samples are part of the JAX-Sen project in the SenNeT Consortium. We aim to study and characterize senescen... | ["[Reagents and Materials] 15 ml conical tubes – 4 per sample\n1.5 or 2 ml Protein lobind tubes\nMicro scissors/ scalpel\nIce-cold PBS\nHistopaque-1077 – Sigma H8889\nWide-bore pipette tips\nCollagenase solution\nWash buffer\n100 µm cell strainer (Corning, 431752)\nACK lysis buffer – 2 ml/sample\nSS_buffer – 5 ml/sampl... |
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