id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
102,586 | “Leveraging AI Tools to Bridge the Healthcare Gap in Rural Areas in India" | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1z9zlmk/v1 | https://www.protocols.io/view/leveraging-ai-tools-to-bridge-the-healthcare-gap-dge23tge | Ajit Kerketta | TITLE: “Leveraging AI Tools to Bridge the Healthcare Gap in Rural Areas in India"
AUTHORS: Ajit Kerketta
[DESCRIPTION]
Introduction:
Despite considerable progress in the healthcare sector, rural areas continue to grapple with healthcare deficiencies, which eventually impact the quality of health outcomes. However,... | ["[“Leveraging AI Tools to Bridge the Healthcare Gap in Rural Areas in India"]"] |
44,745 | Protocols for "Bicolor Angelfish (Centropyge bicolor) genome provided first chromosome-level reference of Pomacanthidae family and clues for bi-color body formation" | 2 | dx.doi.org/10.17504/protocols.io.bpxhmpj6 | https://www.protocols.io/view/protocols-for-34-bicolor-angelfish-centropyge-bico-bpxhmpj6 | Chunhua Li, Xianwei Yang, Libin Shao, Rui Zhang, QunLiu, Mengqi Zhang, Shanshan Liu, Shanshan Pan, Weizhen Xue, Congyan Wang, Chunyan Mao, He Zhang, Guangyi Fan | TITLE: Protocols for "Bicolor Angelfish (Centropyge bicolor) genome provided first chromosome-level reference of Pomacanthidae family and clues for bi-color body formation"
AUTHORS: Chunhua Li, Xianwei Yang, Libin Shao, Rui Zhang, QunLiu, Mengqi Zhang, Shanshan Liu, Shanshan Pan, Weizhen Xue, Congyan Wang, Chunyan Mao,... | [] |
85,663 | P5ARP/P10ARP Media Preparation | 1 | null | https://www.protocols.io/view/p5arp-p10arp-media-preparation-cxv7xn9n | rebecca.bennett | TITLE: P5ARP/P10ARP Media Preparation
AUTHORS: rebecca.bennett
[DESCRIPTION]
How to make P5ARP and P10ARP media plates, simplified from https://wiki.bugwood.org/PARP_or_PARP
Contains: pimarcin, ampicillin, rifampicin, pentachloronitrobenzens.
Pimaricin is a broad-spectrum antifungal antibiotic that inhibits the true... | ["[Preparation] Sign up for Autoclave and turn on.", "[Preparation] Add 17 g of Difco cornmeal agar to each 2 L Erlenmeyer flask", "[Preparation] Add RO water to 1000 mL line (pre-measure line and mark with sharpie or use a graduated cylinder).\nCover top with foil so that at least 2\" of foil is below flask lip.", "[P... |
51,440 | Body composition assessment and sarcopenia in patients with biliary tract cancer: A systematic review and meta-analysis (protocol) | 1 | dx.doi.org/10.17504/protocols.io.bwgqpbvw | https://www.protocols.io/view/body-composition-assessment-and-sarcopenia-in-pati-bwgqpbvw | Jun Watanabe, Ryota Matsui, Hideki Sasanuma, Kazuhiko Kotani, Naohiro Sata | TITLE: Body composition assessment and sarcopenia in patients with biliary tract cancer: A systematic review and meta-analysis (protocol)
AUTHORS: Jun Watanabe, Ryota Matsui, Hideki Sasanuma, Kazuhiko Kotani, Naohiro Sata
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The purpose of the study will ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.d7p9mm | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<span style="color: #333333; font-family: helvetica, sans-serif; font-size: 14px; line-height: 21px;">In order to decompose a complecated metagenomic de Bruijn graph into simpler subgraphs, MetaVelvet searches the following X-structure, i.e., chimeric nodes have two incoming edge... | [] |
42,217 | Immunohistochemical staining of Syndecan-1 (Sdc-1) core proteins in islet beta cells of formalin-fixed human pancreas | 1 | dx.doi.org/10.17504/protocols.io.bmghk3t6 | https://www.protocols.io/view/immunohistochemical-staining-of-syndecan-1-sdc-1-c-bmghk3t6 | Lora Starrs, Debra Brown, Sarah Popp, Charmaine Simeonovic | TITLE: Immunohistochemical staining of Syndecan-1 (Sdc-1) core proteins in islet beta cells of formalin-fixed human pancreas
AUTHORS: Lora Starrs, Debra Brown, Sarah Popp, Charmaine Simeonovic
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Paraffin sections (4 μm thickness) of formalin-fixed human ... | ["See Guidelines, \"Before starting\".", "Deparaffinize slides in each xylene for 1 min. rehydrate slides in graded alcohols beginning in absolute ethanol (10 dips)/ container of absolute ethanol), followed by 90% ethanol (10 dips) and 70% ethanol (10 dips). Wash well in running tap water for 5 min.", "Wipe around sect... |
81,714 | Quantifying Acetylcholinesterase activity in striatal brain tissue with DTNB assay | 4 | dx.doi.org/10.17504/protocols.io.e6nvwj3r7lmk/v1 | https://www.protocols.io/view/quantifying-acetylcholinesterase-activity-in-stria-ct2swqee | Stefania Vietti-Michelina, Stephanie J Cragg | TITLE: Quantifying Acetylcholinesterase activity in striatal brain tissue with DTNB assay
AUTHORS: Stefania Vietti-Michelina, Stephanie J Cragg
[DESCRIPTION]
This protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue, including how to store tissue samples, extract ... | ["[Preparation of ex vivo mouse brain slices] Harvest dorsal and ventral striatal tissue punches (1.2 mm diameter) were from 300 μm thick acute coronal striatal tissue slices, prepared with a vibratome.", "[Preparation of ex vivo mouse brain slices] Equilibrate slices at room temperature for at least 1-hour prior co... |
61,939 | Power Keto Gummies - Lose Weight Faster! | Special Offer! | 3 | dx.doi.org/10.17504/protocols.io.n2bvj6pzwlk5/v1 | https://www.protocols.io/view/power-keto-gummies-lose-weight-faster-special-offe-b8qtrvwn | jeneferloma | TITLE: Power Keto Gummies - Lose Weight Faster! | Special Offer!
AUTHORS: jeneferloma
[DESCRIPTION]
Power Keto Gummies
[STEPS] | [] |
103,324 | NEXTTEC 96 WELL PLATE DNA Extraction EFGL | 0 | dx.doi.org/10.17504/protocols.io.bp2l62381gqe/v1 | https://www.protocols.io/view/nexttec-96-well-plate-dna-extraction-efgl-dg543y8w | EagleFish GeneticsLab | TITLE: NEXTTEC 96 WELL PLATE DNA Extraction EFGL
AUTHORS: EagleFish GeneticsLab
[DESCRIPTION]
The purpose of this extraction protocol is to prepare a DNA sample for later pipeline processing, such as our GT-seq protocol EFGL, SANGER Sequencing EFGL, or RADSeq protocol EFGL. DNA extraction at EFGL occurs via tissue sam... | ["[TISSUE SAMPLING] Gather the items you need to do your EXTRACTION\n a. Extraction sheet/map for tray (Print this sheet from the GTSeq Extraction Template located in the \n project’s folder. The template for Master Extraction Sheets can be found in S:\\Eagle Fish Genetics \n Lab\\LA... |
24,000 | Biochemical Measures of Neuropathy - DHE | null | dx.doi.org/10.17504/protocols.io.3n8gmhw | null | Eva Feldman | TITLE: Biochemical Measures of Neuropathy - DHE
AUTHORS: Eva Feldman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">Oxidative stress is highly correlated with the metabolic changes caused by hyperglycemia. Increased le... | ["Open Fluoroskan and choose open under the file menu. Scroll down and select DHE.sed. Set up your plate layout. Save your layout as DHxxxxxx.sed with xxxxxx being yy/mm/dd.", "*Treat cells per experimental paradigm.Note: *As an alternative, cells can be pre-loaded with DHE- do steps 3, 4, 5, 2, then 5.", "15 minutes p... |
91,487 | Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings | 1 | dx.doi.org/10.17504/protocols.io.n2bvj3jw5lk5/v1 | https://www.protocols.io/view/immunocytochemistry-of-acute-brain-slices-used-in-c5j7y4rn | Yan-Feng Zhang, Stephanie J Cragg | TITLE: Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings
AUTHORS: Yan-Feng Zhang, Stephanie J Cragg
[DESCRIPTION]
The steps detailed in this protocol are to label choline acetyltransferase (ChAT) or tyrosine hydroxylase (TH), which are known markers for cholinergic interneurons (Chl) and... | ["[Fixation] Fix slices in 4% paraformaldehyde (PFA) dissolved in PBS containing 0.2% picric acid.", "[Fixation] Leave to fixate overnight at 4°C and then store in PBS.", "[Fixation] Wash x5 in PBS for 5 min.", "[Labelling Cholinergic Interneurons (ChAT)] Add primary antibody goat anti-ChAT (Millipore, 1:100) or goat a... |
62,628 | C+ Kapseln Kaufen, Test, Preis, Bewertung or Erfahrung | 1 | dx.doi.org/10.17504/protocols.io.5jyl896x6v2w/v1 | https://www.protocols.io/view/c-kapseln-kaufen-test-preis-bewertung-or-erfahrung-b9ecr3aw | Health | TITLE: C+ Kapseln Kaufen, Test, Preis, Bewertung or Erfahrung
AUTHORS: Health
[DESCRIPTION]
C+ Kapseln Kaufen - Sie müssen schnell handeln. Viel mehr Männer kennen die Hauptvorteile des allgemeinen Ausstellung Verstärkers ohne Drogen. Darüber hinaus verwenden sie C+ Kapseln Kaufen Pillen. Daher könnte ein Teil dieser ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.chwt7d | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
88,179 | Janelia Atalanta series plasmid cloning | 4 | dx.doi.org/10.17504/protocols.io.bp2l6bjokgqe/v2 | https://www.protocols.io/view/janelia-atalanta-series-plasmid-cloning-c2ctyawn | David Stern | TITLE: Janelia Atalanta series plasmid cloning
AUTHORS: David Stern
[DESCRIPTION]
The Janelia Atalanta plasmids allows simple cloning of a gRNA and homology arms for CRISPR/Cas9 mediated homology directed repair in Drosophila. Gateway-compatible arms are synthesized with appropriate attL recognition sequences and homo... | ["[Make plasmid DNA] Transform the pJAT into ccdB Survival cells following the standard protocol. Plate cells on LB _ Ampicillin (100ug/mL) and grow at 37°C at least 15 hours.", "[Make plasmid DNA] Pick one colony and grow in 3mL LB + antibiotics. I normally grow in Ampicillin, but the plasmid encodes three antibiotic ... |
30,801 | Enzymatic fingerprinting of AX and mixed-linked BG | null | dx.doi.org/10.17504/protocols.io.babriam6 | null | Alison Lovegrove | TITLE: Enzymatic fingerprinting of AX and mixed-linked BG
AUTHORS: Alison Lovegrove
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Lab protocol for: Enzymatic fingerprinting for arabinoxylan and mixed-linked beta-glucan in wheat flour by HPAEC-PAD. Based on Ordaz-Ortiz and Saulnier, 2005 J. Cereal... | [] |
30,128 | Optimization of blood pressure management after acute ischemic stroke and its prognostic significance: Prospective, randomized, open, blinded outcome evaluation, and feasibility Trial | null | dx.doi.org/10.17504/protocols.io.9nqh5dw | null | Beom Joon Kim | TITLE: Optimization of blood pressure management after acute ischemic stroke and its prognostic significance: Prospective, randomized, open, blinded outcome evaluation, and feasibility Trial
AUTHORS: Beom Joon Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Optimization of blood pressure managem... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.e9gbh3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is part of the <a href="https://www.protocols.io/view/Collection-of-FOCUS-SubCell-Protocols-For-the-Enri-e9ebh3e" target="_blank">collection</a> of FOCUS™ SubCell protocols for the enrichment of subcellular fractions. Please refer to the appropriate protocol depending on... | [] |
80,869 | Organelle isolation from Mouse Embryonic Fibroblasts (MEFs) stably expressing organelle tags for subsequent immunoblotting or proteomic analysis | 4 | dx.doi.org/10.17504/protocols.io.ewov1o627lr2/v1 | https://www.protocols.io/view/organelle-isolation-from-mouse-embryonic-fibroblas-cs8dwhs6 | Matthew Taylor, Pui Yiu Lam, Francesca Tonelli, Dario R Alessi | TITLE: Organelle isolation from Mouse Embryonic Fibroblasts (MEFs) stably expressing organelle tags for subsequent immunoblotting or proteomic analysis
AUTHORS: Matthew Taylor, Pui Yiu Lam, Francesca Tonelli, Dario R Alessi
[DESCRIPTION]
We describe here a method to perform rapid isolation of intact organelles (includ... | ["[Isobiotec cell-breaker assembly] Insert the metal ball of choice inside the cell breaker.", "[Isobiotec cell-breaker assembly] Screw the lids on tightly.", "[Isobiotec cell-breaker assembly] Push 3 mL of KPBS through the cell breaker to wash it.", "[Isobiotec cell-breaker assembly] Carefully tap dry.", "[Isobiotec c... |
null | null | null | dx.doi.org/10.17504/protocols.io.etkbekw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
These protocols were developed to show the features of the protocols.io platform and how groups like VERVE Net can implement future protocol design based from the mock and technical protocols written. Each author wrote one specific protocol to model applications of protocols.io ... | [] |
28,513 | Identify fetal yawns based on temporal dynamics of mouth opening. | null | dx.doi.org/10.17504/protocols.io.739hqr6 | null | Damiano Menin, Angela Costabile, Flaviana Tenuta, Harriet Oster, Marco Dondi | TITLE: Identify fetal yawns based on temporal dynamics of mouth opening.
AUTHORS: Damiano Menin, Angela Costabile, Flaviana Tenuta, Harriet Oster, Marco Dondi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In these two studies, we examined the temporal dynamics of yawning and non-yawn mouth openin... | ["[Procedure and video-recording]\nNeonates were observed while they were lying supine in a cot. Behavior was video-recorded (24 frames per second) for 10 to 30 minutes (M =18.63, SD = 6.31), at a midpoint in the feeding cycle, when the neonates were not receiving any stimulation through routine nursing or medical care... |
20,464 | Suppement data in AJPGI-00061-2018R2 | null | dx.doi.org/10.17504/protocols.io.x8qfrvw | null | Jian Tu, Ting Xiong | TITLE: Suppement data in AJPGI-00061-2018R2
AUTHORS: Jian Tu, Ting Xiong
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Methods and results of supplement figure 1</div></div>
[STEPS]
?. resultslegendsmethods | ["resultslegendsmethods"] |
92,159 | Nucleic acids extraction from single cell using MasterPure Complete DNA purification (Epicenter) | 1 | dx.doi.org/10.17504/protocols.io.x54v9mk3qg3e/v3 | https://www.protocols.io/view/nucleic-acids-extraction-from-single-cell-using-ma-c587y9zn | Sarah Romac | TITLE: Nucleic acids extraction from single cell using MasterPure Complete DNA purification (Epicenter)
AUTHORS: Sarah Romac
[DESCRIPTION]
Radiolaria are protists which can't be cultivated. These microoganisms have to be isolated by single-cell for genetic identification and it can be difficult to get clean DNA.
Here ... | ["[1. Cell Isolation] Isolate individually protist cells (at least 50µm in length) using a glass bent micropipette under a binocular microscope.", "[1. Cell Isolation] Wash each cell in three successive baths of 0.22µm-filtered and sterile seawater.", "[1. Cell Isolation] Transfer subsequently cells in a 1.5mL... |
null | null | null | dx.doi.org/10.17504/protocols.io.chct2v | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to <a href="High-Efficiency-Transformation-Protocol-using-NEB-imstq5" target="_blank">this protocol</a>.
[GUIDELINES]
<strong>Transformation Protocol Variables</stron... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.g89bzz6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Contact Dr. Steven Wilhelm (wilhelm@utk.edu) or Robbie Martin (rmarti49@vols.utk.edu) for additional information regarding this protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
106,610 | Induced Cortical Neuron Differentiation - Part 1 - Overview | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8yoolmk/v1 | https://www.protocols.io/view/induced-cortical-neuron-differentiation-part-1-ove-dkcs4swe | Isabel Lam, Alain Ndayisaba, Vikram Khurana | TITLE: Induced Cortical Neuron Differentiation - Part 1 - Overview
AUTHORS: Isabel Lam, Alain Ndayisaba, Vikram Khurana
[DESCRIPTION]
Induced Cortical Neuron Differentiation - Part 1 - Overview
[STEPS] | [] |
59,312 | Useful methods 4: Stock cultivation of duckweed | 1 | null | https://www.protocols.io/view/useful-methods-4-stock-cultivation-of-duckweed-b56qq9dw | K. Sowjanya Sree, Klaus-J. Appenroth | TITLE: Useful methods 4: Stock cultivation of duckweed
AUTHORS: K. Sowjanya Sree, Klaus-J. Appenroth
[DESCRIPTION]
This protocol details about stock cultivation of duckweed. It contains protocols from the The International Steering Committee on Duckweed Research and Application (ISCDRA) Newsletter. A complete list of ... | ["[Stock cultivation of duckweed] For stock cultivation we reduce the temperature to 18 °C. All the duckweed species can deal with this temperature, of course with a dramatically reduced growth rate. Even 15 °C is possible. The late Elias Landolt mentioned that clones collected from tropical climates cannot tolerate 5 ... |
88,813 | Carver et al, Aged Brain Spatial Profiling - CosMx | 1 | dx.doi.org/10.17504/protocols.io.dm6gp3yb5vzp/v1 | https://www.protocols.io/view/carver-et-al-aged-brain-spatial-profiling-cosmx-c2ymyfu6 | Chase Carver | TITLE: Carver et al, Aged Brain Spatial Profiling - CosMx
AUTHORS: Chase Carver
[DESCRIPTION]
This protocol provides the process for spatial molecular imaging using the Nanostring CosMx platform used in
Carver et al., "Senescent- and disease-associated microglia are modifiable features of aged brain white matter".
[... | ["[CosMx Slide Preparation] Using FFPE sections, follow the protocol found in the document", "[CosMx Instrument run] Follow the protocol found in the document \nfor imaging of flow cells, FOV selection, and running spatial molecular imaging on the instrument.\nThe Cell Segmentation Profile used for mouse brain RNA is ... |
39,669 | SOLUTION- 01 - Sodium Chloride (NaCl) solution | 3 | dx.doi.org/10.17504/protocols.io.biyvkfw6 | https://www.protocols.io/view/solution-01-sodium-chloride-nacl-solution-biyvkfw6 | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: SOLUTION- 01 - Sodium Chloride (NaCl) solution
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[STEPS] | [] |
66,882 | ADP Glo Max measuring ATP13A2 ATPase activity in microsomes | 6 | dx.doi.org/10.17504/protocols.io.6qpvr65ozvmk/v1 | https://www.protocols.io/view/adp-glo-max-measuring-atp13a2-atpase-activity-in-m-cdjas4ie | Sue Sim, eunyong_park | TITLE: ADP Glo Max measuring ATP13A2 ATPase activity in microsomes
AUTHORS: Sue Sim, eunyong_park
[DESCRIPTION]
Measuring ATP13A2 ATPase activity in microsomes using commercial luminescence-based ADP detection assay (ADP Glo Max; Promega)
[STEPS]
1. Thaw microsomes on ice
2. Use 1 μg microsomes per reaction (1 µL ... | ["Thaw microsomes on ice", "Use 1 μg microsomes per reaction (1 µL of 5 mg/mL stock)", "Dilute to 4 µL in Reaction Buffer at 4C", "Equilibrate microsomes in reaction buffer for 90 min on ice and for 10 min at 37 °C", "Add 1 µL of 25 mM ATP (final concentration 5 mM ATP) to start reaction (total reaction volume 5 µL)", ... |
72,542 | Muconic acid isomers and aromatic compounds analyzed by UHPLC-DAD | 1 | dx.doi.org/10.17504/protocols.io.36wgqjjxyvk5/v1 | https://www.protocols.io/view/muconic-acid-isomers-and-aromatic-compounds-analyz-ci36ugre | Stefan J. Haugen, William E. Michener, Sean P. Woodworth, Kelsey J. Ramirez, Gregg T. Beckham | TITLE: Muconic acid isomers and aromatic compounds analyzed by UHPLC-DAD
AUTHORS: Stefan J. Haugen, William E. Michener, Sean P. Woodworth, Kelsey J. Ramirez, Gregg T. Beckham
[DESCRIPTION]
An analysis method was developed to allow for quantitation of muconic acid isomers (cis, cis and cis, trans) and aromatic compou... | ["[Preparation of Standards] Calibration Curve", "[Analytical Quality Control] Multiple strategies are utilized when performing this analysis to ensure instrument stability and reproducibility.", "[Preparation of Standards] Standards\n\n This procedure for standard preparation is previously documented in our work publi... |
68,773 | Using Github to create a eNotebook | 5 | null | https://www.protocols.io/view/using-github-to-create-a-enotebook-cfedtja6 | Alise J J. Ponsero | TITLE: Using Github to create a eNotebook
AUTHORS: Alise J J. Ponsero
[DESCRIPTION]
Instructions to create a Github Account and your first repository.
[STEPS]
SECTION: Create an account
1. Browse to the Github website
https://github.com/
SECTION: Create an account
2. Click on the Sign-up button on the right corner of... | ["[Create an account] Browse to the Github website\nhttps://github.com/", "[Create an account] Click on the Sign-up button on the right corner of the screen.", "[Create an account] Follow the instructions to create your account.\n\nTo the Qestion 1, answer that you are working alone and that you are a student.", "[Crea... |
42,388 | CRISPRon-GFP (based on pX459 plasmid) cloning of guides | 4 | null | https://www.protocols.io/view/crispron-gfp-based-on-px459-plasmid-cloning-of-gui-bmmuk46w | Ofer Barnea-Yizhar | TITLE: CRISPRon-GFP (based on pX459 plasmid) cloning of guides
AUTHORS: Ofer Barnea-Yizhar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol was written in accordance in accordance with the Zheng Lab protocol and the following lessons learned from the following papers.</div></div>
[STEP... | ["[Vector digest]\nDigest of an empty vector with (the enzyme is kept in -80 REVCO). Comprise the reaction as follows: AB1Vector (dervided from CRIPSRon) 1-1.5 ug ( X uL) 2 BbsI enzyme 1 uL 3 NEB buffer 2.1 5 uL 4 ddH2O up to 50 uL 5 TOTAL 50 uL\n1 µg\nAB1Vector (dervided from CRIPSRon) 1-1.5 ug ( X uL) 2 BbsI... |
null | null | null | dx.doi.org/10.17504/protocols.io.gwebxbe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Cluster genomes is script that clusters genomes at a set nucleotide identity and coverage length. Additonally, it offers the ability to cluster sequences whose ends may not align correspondingly, i.e. the special cases of assembled, circular viral genomes that are treated as ... | [] |
32,935 | Purifying DNA from Agarose with Homemade Glass Milk | null | dx.doi.org/10.17504/protocols.io.bcefitbn | null | Joanne Kamens | TITLE: Purifying DNA from Agarose with Homemade Glass Milk
AUTHORS: Joanne Kamens
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>You don't need an expensive kit to purify DNA. I used to make my own (very very cheap) glass milk from this protocol. It's hard to find online these days so I'm add... | ["Preparaton of Glass Powder (Steps 1-4)Resuspend 400 g of glass powder in 800 ml double distilled (dd) H20 in a 2 liter flask with a stir barStir for 60 minutes", "Collect the \"fines\"Remove and keep the supernatant by gentle pouring, leaving all settled glass. This contains fine particles which is what you want to c... |
41,614 | FloodLAMP LAMP Assay Protocol v4.1 (0.5ml, Strips, Multichannel) | 4 | dx.doi.org/10.17504/protocols.io.bkvnkw5e | https://www.protocols.io/view/floodlamp-lamp-assay-protocol-v4-1-0-5ml-strips-mu-bkvnkw5e | Randy True | TITLE: FloodLAMP LAMP Assay Protocol v4.1 (0.5ml, Strips, Multichannel)
AUTHORS: Randy True
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>FloodLAMP uses </span><a href="https://www.medrxiv.org/content/10.1101/2020.04.23.20076877v1" style = "text-decoration:underline;color:blue;cursor:pointer... | ["[Purification ( .5mL sample)]\nSpike selected samples with Twist RNA", "[Purification ( .5mL sample)]\nFor each 8 samples, Add 45uL of glass milk (GM) to 2.25mL of binding solution (BS), vortex glass milk before using, flicking tube to be sure that there is no pellet at the bottomand it's evenly mixed", "[Purificatio... |
91,188 | Non-enzymatic passaging of hPSC | 4 | dx.doi.org/10.17504/protocols.io.5qpvo32k7v4o/v1 | https://www.protocols.io/view/non-enzymatic-passaging-of-hpsc-c5auy2ew | Valeria Fernandez Vallone, Lyn Healy, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Lisa Pavinato, Fatma Visal FVO OKUR, Harald Stachelscheid | TITLE: Non-enzymatic passaging of hPSC
AUTHORS: Valeria Fernandez Vallone, Lyn Healy, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Lisa Pavinato, Fatma Visal FVO OKUR, Harald Stachelscheid
[DESCRIPTION]
This protocol describes the procedure to passage hPSC culture using enzyme-free reagents.
[BEFORE_START]
If usi... | ["[Preparation of destination vessel] Prepare coated tissue culture vessels and culture media according to hPSC line requirements and desired format. \nRefer to protocols: Coating of tissue Culture Vessels for hPSC and Maintenance of hPSC.", "[hPSC non-enzymatic passaging] Aspirate and discard media from selected sourc... |
null | null | null | dx.doi.org/10.17504/protocols.io.dtn6md | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
A short and relatively cheap method for non-homologous nuclear transformation of Chlamydomonas reinhardtii. Works best with linearized DNA. Requires 500 micron glass beads for DNA transformation.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
50,708 | Single cell digestion of tumor tissue | 4 | dx.doi.org/10.17504/protocols.io.bvrun56w | https://www.protocols.io/view/single-cell-digestion-of-tumor-tissue-bvrun56w | Shubhangi Agarwal, donna.peehl , Renuka Sriram | TITLE: Single cell digestion of tumor tissue
AUTHORS: Shubhangi Agarwal, donna.peehl , Renuka Sriram
[DESCRIPTION]
The purpose of this protocol is to provide details on how to obtain a single-cell suspension from a patient-derived xenograft tumor.
[STEPS]
SECTION: Preparation of Medium
1. Prepare digestion me... | ["[Preparation of Medium] Prepare digestion medium \nDMEM supplemented with\na. 10 % volume FBS,\nb. 200 units/ml of type I collagenase, \nc. 1 units/ml DNase I, \nd. 2 Micromolar (µM) Y-27632 and \ne. 100 ug/ml Gentamicin", "[Preparation of Medium] Prepare storage medium\nDMEM supplemented with\na. 10 % volume FBS,... |
82,657 | Building a SpikeGLX Rig with camera: Chronic recoverable Neuropixels in mice | 1 | dx.doi.org/10.17504/protocols.io.kxygxzzxkv8j/v3 | https://www.protocols.click/view/building-a-spikeglx-rig-with-camera-chronic-recove-cux9wxr6 | Emily A Aery Jones | TITLE: Building a SpikeGLX Rig with camera: Chronic recoverable Neuropixels in mice
AUTHORS: Emily A Aery Jones
[DESCRIPTION]
This protocol collection explains how to build a low-cost, lightweight system to implant 1 Neuropixels 1.0 probe or 2 Neuropixels 2.0 probes into mice, record during freely moving behavior, the... | ["[Set up acquisition hardware] Load the PCIe module into your computer", "[Set up acquisition hardware] Load the PXIe modules and the IMEC module into the NIDAQ chassis", "[Set up acquisition hardware] Connect the PXIe modules to the BNC breakout board and the computer. Connect the IMEC SMA to the BNC board (analog sl... |
null | null | null | dx.doi.org/10.17504/protocols.io.ibvcan6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The protocol describes our attempt to transform three marine protists by biolistic.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
100,194 | Multiplex Labeling with Tyramide Fluorophores for Detecting CIAP-Resistant PSER129 and Proteinase K-Resistant aSyn in situ (Killinger 2024) | 0 | dx.doi.org/10.17504/protocols.io.x54v92dq4l3e/v1 | https://www.protocols.io/view/multiplex-labeling-with-tyramide-fluorophores-for-dd4a28se | Solji Choi, Bryan Killinger | TITLE: Multiplex Labeling with Tyramide Fluorophores for Detecting CIAP-Resistant PSER129 and Proteinase K-Resistant aSyn in situ (Killinger 2024)
AUTHORS: Solji Choi, Bryan Killinger
[DESCRIPTION]
This protocol aims to examine the association of calf-intestinal alkaline phosphatase (CIAP)-resistant alpha-synuclein ph... | ["[Day 1] Wash free-floating tissue (3 x 10 minutes) in dilution media (DM).\n\nDilution media:", "[Day 1] Wash free-floating tissue for 10 min in dilution media (DM). (1/3)", "[Day 1] Wash free-floating tissue for 10 min in dilution media (DM). (2/3)", "[Day 1] Wash free-floating tissue for 10 min in dilution media (D... |
97,014 | Macroalgae Analysis | 0 | dx.doi.org/10.17504/protocols.io.4r3l22xypl1y/v2 | https://www.protocols.io/view/macroalgae-analysis-dayw2fxe | Nohea Rodriguez, Kai Bloom, Mistie Tran | TITLE: Macroalgae Analysis
AUTHORS: Nohea Rodriguez, Kai Bloom, Mistie Tran
[DESCRIPTION]
Determine the abundance and biomass of the invasive macroalgae species, Turbinaria and Sargassum. Then, identify marine species located on random samples of each species. Turbinaria decreases fish abundance and biodiversity, comp... | ["[Algae Transect Survey] Split into five groups of four people.", "[Algae Transect Survey] Swim to the group's transect location, approximately 5 meters apart.", "[Algae Transect Survey] Begin 30 meter transect along reef crest, parallel to the shore.", "Swim along transect tape and record the presence of rock, coral,... |
67,166 | What Is The BeLiv Blood Sugar Oil ! | 1 | dx.doi.org/10.17504/protocols.io.36wgq78yyvk5/v1 | https://www.protocols.io/view/what-is-the-beliv-blood-sugar-oil-cdt6s6re | belivreport | TITLE: What Is The BeLiv Blood Sugar Oil !
AUTHORS: belivreport
[DESCRIPTION]
Many individuals with diabetes battle with fluctuating glucose levels. Diabetes medications and insulin shots can help. Be that as it may, certain individuals with diabetes search for regular choices to help glucose. As indicated by the US... | [] |
97,045 | Electrolyte Leakage Assay to Analyze Membrane Integrity in Leaves | 0 | dx.doi.org/10.17504/protocols.io.ewov19j37lr2/v1 | https://www.protocols.io/view/electrolyte-leakage-assay-to-analyze-membrane-inte-dazv2f66 | Laura Tovar-Rosales, Manoj-Kumar Arthikala, Kalpana Nanjareddy | TITLE: Electrolyte Leakage Assay to Analyze Membrane Integrity in Leaves
AUTHORS: Laura Tovar-Rosales, Manoj-Kumar Arthikala, Kalpana Nanjareddy
[DESCRIPTION]
The production of reactive oxygen species occurs naturally in certain cellular organelles as metabolic byproducts. However, under stress conditions, their accum... | ["[Preparation of Samples] Using a cork borer, cut leaf disks of the desired diameter (at least 5 mm) from one plant, ensuring at least one disk per leaf. Perform this process on sterilized paper towels. With the assistance of fine forceps and gloves, carefully detach the disks from the vascular bundles.", "[Preparatio... |
85,540 | Immunohistochemistry (IHC) Staining Mouse Brain Sections | 1 | dx.doi.org/10.17504/protocols.io.5qpvo3b7bv4o/v1 | https://www.protocols.io/view/immunohistochemistry-ihc-staining-mouse-brain-sect-cxscxnaw | Naveen Ouellette, daphne.toglia, Holly Myers | TITLE: Immunohistochemistry (IHC) Staining Mouse Brain Sections
AUTHORS: Naveen Ouellette, daphne.toglia, Holly Myers
[DESCRIPTION]
The Immunohistochemistry (IHC) Staining for Mouse Brain Sections protocol details the blocking, primary, and secondary antibody staining of 50-100 micron mouse brain tissue slices fixed i... | ["[Staining] Wash sections in 1xPBS & 0.01% Azide three times for 5 min in well plate or Netwell cell culture inserts on shaker at Room temperature .", "[Staining] Blocking:", "[Staining] Choose a blocking solution:", "[Staining] Chose a blocking duration based on section thickness. Note that the following times are mi... |
79,956 | Isolation of natural killer (NK) cells from human blood products | 1 | dx.doi.org/10.17504/protocols.io.81wgbp94yvpk/v2 | https://www.protocols.click/view/isolation-of-natural-killer-nk-cells-from-human-bl-csbuwanw | Philippa R Kennedy | TITLE: Isolation of natural killer (NK) cells from human blood products
AUTHORS: Philippa R Kennedy
[DESCRIPTION]
Standard isolation procedure for peripheral blood mononuclear cells (PBMC) from human blood leukopheresis products.
[GUIDELINES]
One trimacone will generally provide 500 million - 2 billion PBMCs.
NK c... | ["[Overview] Healthy donor blood products (Leukopaks) were obtained from Memorial Blood Bank (Minneapolis, MN). All samples were de-identified and their use was approved by the University of Minnesota and NMDP institutional review board in accordance with the Declaration of Helsinki.", "[Overview] Peripheral blood mono... |
60,599 | USER MANUAL of U-uF-BM# Bifurcating Mixing Microfluidic Chips | 4 | dx.doi.org/10.17504/protocols.io.yxmvmnyn9g3p/v2 | https://www.protocols.io/view/user-manual-of-u-uf-bm-bifurcating-mixing-microflu-b7exrjfn | Serhat S | TITLE: USER MANUAL of U-uF-BM# Bifurcating Mixing Microfluidic Chips
AUTHORS: Serhat S
[DESCRIPTION]
This is the user manual for U-uF-BM# Bifurcating Mixing Microfluidic Chips of uFluidic.com.
It is designed to make nanoencapsulation liposomes and polimeric nanoparticles.
[GUIDELINES]
There are no particular guideli... | ["[INTRODUCTION] THIS IS A BIFURCATING MIXING MICRO-CHANNEL. MAINLY DESIGNED FOR LIPOSOME AND NANO-PARTICLE GENERATION AND DRUG-VACCINE ENCAPSULATION PURPOSES.\n\nDESCRIPTION\n\nBy laminar flow microfluidic channels, it is mostly accepted that these two were very hard;\nrapid mixing of liquids\nhigh volume fabrication\... |
20,973 | Microwell-based Single-Cell RNA-seq | null | dx.doi.org/10.17504/protocols.io.yqmfvu6 | null | Peter Sims, Jinzhou Yuan, Yim L. Cheng | TITLE: Microwell-based Single-Cell RNA-seq
AUTHORS: Peter Sims, Jinzhou Yuan, Yim L. Cheng
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">(</span><span style = "font-weight:bold;">On</span><span>-chip reverse transcription version, Sims Lab – Jinzhou Yuan and Yim L... | ["[Experiment day 1]\nLive stain cellsHarvest and resuspend 500,000 cells in (500 cells/uL).\n[TBS]", "[Experiment day 1]\nIncubate the cells in 4 uM CalceinAM (4ul 1mM CalceinAM per ml cells) for on ice.", "[Experiment day 1]\nPreparation of lysis buffer, and wash buffer, and fluorinated oilWhile the cells are being ... |
91,949 | RSVAB WGS and GF protocols | 1 | dx.doi.org/10.17504/protocols.io.kqdg3xbzqg25/v2 | https://www.protocols.io/view/rsvab-wgs-and-gf-protocols-c52my8c6 | miglesias | TITLE: RSVAB WGS and GF protocols
AUTHORS: miglesias
[DESCRIPTION]
This SOP describes the procedure for generating cDNA from RSV viral nucleic acid extracts and subsequently producing amplicons tiling the viral genome using. We propose two systems for genomic characterization of RSV. First, a novel RSV amplicon-based ... | ["[dsCDNA generation:] During this step three master mixes will be prepared: MMI, MMII and MMIII.\nMaterials: Kit Superscript III First Strand (Invitrogen)\n 100% DMSO\n RNAseH (Invitrogen)\n Klenow fragment 3' ->5' exo (New England Biolab... |
52,233 | DNA extraction and precipitation | 4 | null | https://www.protocols.io/view/dna-extraction-and-precipitation-bw9hph36 | Marco Cacciabue, María Ines Gismondi | TITLE: DNA extraction and precipitation
AUTHORS: Marco Cacciabue, María Ines Gismondi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Briefly, the DNA solution is extracted with phenol / chloroform / isoamyl alcohol in a ratio of 25/24/1 to remove protein contaminants and then precipitated with 100... | ["Add 1 volume of 25/24/1 phenol / chloroform / isoamyl alcohol.", "Mix by vortexing for or more at .\nCentrifuge: 13000 33, 15 s\n0 Room temperature", "Take the aqueous phase (the upper one) using a pipette; transfer to new tube.\n200 µl", "Add 1/10 of 3M sodium acetate,. And mix by vortexing or tapping. An alternat... |
46,866 | Purification and quantification from PCR amplification protocol | 4 | dx.doi.org/10.17504/protocols.io.brzsm76e | https://www.protocols.io/view/purification-and-quantification-from-pcr-amplifica-brzsm76e | Rene Flores Clavo, Nataly Ruiz Quinones, Cristian Daniel Asmat Ortega | TITLE: Purification and quantification from PCR amplification protocol
AUTHORS: Rene Flores Clavo, Nataly Ruiz Quinones, Cristian Daniel Asmat Ortega
[STEPS]
?. [Purification steps]
Prepare collection tubes for each sample with their corresponding columns
?. [Purification steps]
Add 500 μL of capture buffer on each c... | ["[Purification steps]\nPrepare collection tubes for each sample with their corresponding columns", "[Purification steps]\nAdd 500 μL of capture buffer on each column", "[Purification steps]\nAdd 25 μL of PCR-amplified DNA", "[Purification steps]\nSlowly mix the solution using a micropipette.", "[Purification steps]\nC... |
39,311 | Collection, Packaging and Cold Shipping of Fresh Non-Islet Pancreatic (Acinar) Tissue | 4 | dx.doi.org/10.17504/protocols.io.bimpkc5n | https://www.protocols.io/view/collection-packaging-and-cold-shipping-of-fresh-no-bimpkc5n | Integrated Islet Distribution Program | TITLE: Collection, Packaging and Cold Shipping of Fresh Non-Islet Pancreatic (Acinar) Tissue
AUTHORS: Integrated Islet Distribution Program
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SOP defines a standardized method for packaging and cold shipping of research quality, fresh Non-Islet Panc... | ["[Preparation of Supplies and Reagents]\nThe IIDP will provide each center with the following supplies necessary for NIPT fresh shipping:Gemini Human AB Serum (ABS) -Heat Inactivated (HI)PIM(G)® - 5 mL – Glutamine/GlutathionePIM(T)® - 500 mL – Transport MediaCiprofloxacin Hydrochloride. Either MP Biomedicals™ MP219902... |
93,365 | A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols | 2 | dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1 | https://www.protocols.io/view/a-rab7a-phosphoswitch-coordinates-rubicon-homology-c7evzje6 | Dan Tudorica | TITLE: A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols
AUTHORS: Dan Tudorica
[DESCRIPTION]
Methods used in this paper.
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.tubensn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
These laboratory protocols contain more detailed information on the experimental methods of study 1 and 2 of the paper Dual Pathways to Voice Quality.
The paper investigates the involvement of Working Memory Capacity (WMC, the cognitive resource necessary for controlled elabo... | [] |
103,546 | Resuspension, Purification, and Preparation of Working Aliquots of Oligos | 0 | dx.doi.org/10.17504/protocols.io.x54v92341l3e/v1 | https://www.protocols.io/view/resuspension-purification-and-preparation-of-worki-dhc232ye | Noé Macias, Alejandra Montoya Rosales | TITLE: Resuspension, Purification, and Preparation of Working Aliquots of Oligos
AUTHORS: Noé Macias, Alejandra Montoya Rosales
[DESCRIPTION]
This protocol details the steps for resuspending, purifying, and preparing working aliquots of oligonucleotides (oligos) for use in molecular biology experiments. The procedure ... | ["[Resuspension, Purification, and Preparation of Working Aliquots of Oligos] RESUSPENSION\nRecord all data or attach the technical sheet of the oligo in the logbook.\nEnsure the lyophilized tube is well-sealed and centrifuge for 1 minute at 5000 rpm.\nIn a sterile, airflow-free hood, open the tube and add 200 µL of st... |
null | null | null | dx.doi.org/10.17504/protocols.io.nvude6w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>In this paper, an adaptive fractional order sliding mode control (AFSMC) scheme is designed for the current tracking control of the Boost-type converter in a Battery/Supercapacitor hybrid energy storage system (HESS). In order to stabilize the current, the adaptation rules ba... | [] |
96,905 | Cathepsin D assay to verify the retention of lysosomal content | 0 | dx.doi.org/10.17504/protocols.io.8epv5r7j5g1b/v1 | https://www.protocols.io/view/cathepsin-d-assay-to-verify-the-retention-of-lysos-davh2e36 | Rotimi Y. Fasimoye, Dario R. Alessi | TITLE: Cathepsin D assay to verify the retention of lysosomal content
AUTHORS: Rotimi Y. Fasimoye, Dario R. Alessi
[DESCRIPTION]
Cathepsin D assay is a fluorescence-based assay that leverage on the activity of cathepsin D, a lysosomal enzyme, to monitor the intactness of lysosome in the cell. Here, we describe a metho... | ["[Seeding cells and performing Lyso-IP with anti-TMEM192 beads] Seed HEK293 cells in 15cm plates and allow to reach 80-90% confluency.", "[Seeding cells and performing Lyso-IP with anti-TMEM192 beads] Perform Lyso-IP (using anti-TMEM192 beads) and Mock-IP (using BSA coated beads) as previously described in dx.doi.org/... |
null | null | null | dx.doi.org/10.17504/protocols.io.jtmcnk6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes isolation of PBMC from human whole peripheral blood samples by density gradient centrifugation, using One SepMate-50ml tube (<a href="https://www.stemcell.com/sepmate-trade-hassle-free-pbmcs-in-just-15-minutes.html" target="_blank">https://www.stemcell... | [] |
74,540 | Chile atole verde - test for draft posting | 1 | null | https://www.protocols.io/view/chile-atole-verde-test-for-draft-posting-ck2kuycw | Gabriel Gasque | TITLE: Chile atole verde - test for draft posting
AUTHORS: Gabriel Gasque
[DESCRIPTION]
Test for draft posting
[STEPS]
SECTION: Salsa verde
1. Asa el chile poblano y los chiles jalapeños. No es necesario pelar los chiles.
SECTION: Chileatole
5. Sofríe la cebolla por 3 min o hasta que esté translúcida y sin dorars... | ["[Salsa verde] Asa el chile poblano y los chiles jalapeños. No es necesario pelar los chiles.", "[Chileatole] Sofríe la cebolla por 3 min o hasta que esté translúcida y sin dorarse.", "[Chileatole] Agrega el ajo y cocina por 1 min.", "[Salsa verde] Licúa los chiles asados con la espinaca, la hoja santa y las 6 hojas d... |
99,224 | LC-MS of Native Nanodiscs | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1k97lmk/v2 | https://www.protocols.io/view/lc-ms-of-native-nanodiscs-dc5y2y7w | Caroline Brown, Snehasish Ghosh, Kallol Gupta | TITLE: LC-MS of Native Nanodiscs
AUTHORS: Caroline Brown, Snehasish Ghosh, Kallol Gupta
[DESCRIPTION]
This protocol provides a step-by-step guide for conducting LC-MS analysis of native nanodiscs extracted from biological membranes.
[STEPS]
SECTION: Liquid Chromatography
1. Resuspend peptides in water + 0.1 formic ac... | ["[Liquid Chromatography] Resuspend peptides in water + 0.1 formic acid varying amount of water so that 500ng of peptide can be loaded in a reasonable volume.", "[Liquid Chromatography] Chromatography is subsequently conducted using home-packed C18 columns (15cm x 75uM ID) for separation of peptides by hydrophobicity."... |
null | null | null | dx.doi.org/10.17504/protocols.io.dju4nv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in "<a href="https://www.protocols.io/view/SYBR-Green-Assay-djg4jv" target="_blank">SYBR Green Assay</a>".
[STEPS]
?.
?.
?.
?. | [] |
52,885 | FindingNemo Extraction 3: NEB Monarch Kit | 1 | dx.doi.org/10.17504/protocols.io.bxvvpn66 | https://www.protocols.io/view/findingnemo-extraction-3-neb-monarch-kit-bxvvpn66 | Inswasti Cahyani, John Tyson, Nadine Holmes, Josh Quick, Nicholas Loman, Matthew Loose | TITLE: FindingNemo Extraction 3: NEB Monarch Kit
AUTHORS: Inswasti Cahyani, John Tyson, Nadine Holmes, Josh Quick, Nicholas Loman, Matthew Loose
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is a sub-protocol designed to extract/isolate ultra-high molecular weight (UHMW) DNA to obtain u... | ["[UHMW DNA Extraction]\nThis protocol is using Monarch® HMW DNA Extraction Kit for Cells & Blood.", "[Cell Lysis]\nPellet 3 million cells in a 1.5 ml tube by centrifuging at 1000 x g for 1 min.\nCentrifuge: 1000 34, 1 min", "[Cell Lysis]\nWash with cold PBS (make sure all media and serum are rinsed off), spin at 1000 ... |
null | null | null | dx.doi.org/10.17504/protocols.io.jiuckew | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
63,414 | Biologic Trim Keto Gummies - Melt all layers of solvent fat of the body! Survey | 3 | dx.doi.org/10.17504/protocols.io.e6nvwk29dvmk/v1 | https://www.protocols.io/view/biologic-trim-keto-gummies-melt-all-layers-of-solv-b96wr9fe | Acv keto Gummies | TITLE: Biologic Trim Keto Gummies - Melt all layers of solvent fat of the body! Survey
AUTHORS: Acv keto Gummies
[DESCRIPTION]
Biologic Trim Keto Gummies
[STEPS] | [] |
26,656 | Assessment of Physiotherapy Practice-Chinese to evaluate student performance during clinical placement | 1 | dx.doi.org/10.17504/protocols.io.598g99w | https://www.protocols.io/view/assessment-of-physiotherapy-practice-chinese-to-ev-598g99w | Hu Jia, Alice YM Jones, Zhou Xuelian, Zhai Hua, Ngai PC Shirley, Siu Ka-Chun Joseph, Dalton Megan | TITLE: Assessment of Physiotherapy Practice-Chinese to evaluate student performance during clinical placement
AUTHORS: Hu Jia, Alice YM Jones, Zhou Xuelian, Zhai Hua, Ngai PC Shirley, Siu Ka-Chun Joseph, Dalton Megan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This project describes the process ... | ["Two physiotherapy academic staff from the involved university who received their education in China and English-speaking countries translated the English APP into Mandarin Chinese with Simplified Chinese characters (Chinese version 1).", "This version was then reviewed by a third bilingual physiotherapy educator (AJ)... |
null | null | null | dx.doi.org/10.17504/protocols.io.ehnbb5e | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
98,166 | FUNDIS ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding Flongle 10.4.1 | 4 | null | https://www.protocols.io/view/fundis-ont-v14-nanopore-adapter-ligation-for-funga-db4w2qxe | Stephen Douglas Russell, Harte Singer | TITLE: FUNDIS ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding Flongle 10.4.1
AUTHORS: Stephen Douglas Russell, Harte Singer
[DESCRIPTION]
This process will take your A-tailed library and add the nanopore adapters. Simply combine several chemicals for a single reaction and do a bead cleanup.
Tested with:
Fl... | ["[Adapter Ligation Flongle 10.4.1] You should already have your reagents on ice from the dA tailing step, but if not you will need the NEBNext Quick Ligation Module enzyme and from the ONT kit you will need 1 tube of SFB, and LA, LNB, EB, and AXP. Spin down the NEB enzyme and immediately place on ice. Thaw AXP and all... |
68,775 | Non-destructive DNA extraction from DESS preservation solution | 4 | null | https://www.protocols.io/view/non-destructive-dna-extraction-from-dess-preservat-cfeftjbn | Eri Ogiso-Tanaka, Minako Abe Ito, Daisuke Shimada | TITLE: Non-destructive DNA extraction from DESS preservation solution
AUTHORS: Eri Ogiso-Tanaka, Minako Abe Ito, Daisuke Shimada
[DESCRIPTION]
DESS is a widely used storage solution to preserve DNA in biological tissue samples. DESS consists of 20% dimethyl sulfoxide (DMSO), 250 mM ethylenediaminetetraacetic acid (ED... | ["Sample storage using DESS solution (see Materials page)\n\nTo transport or store the DNA as undamaged as possible, place the specimen or environmental sample in DESS solution.\n\n*Cautions when the sample is an animal\nSince the DESS solution has low toxicity, animals are not immediately killed when placed in the DES... |
53,115 | Symbiont density quantification in live Aiptasia | 1 | null | https://www.protocols.io/view/symbiont-density-quantification-in-live-aiptasia-bx43pqyn | Shumpei Maruyama, OSU Weis Lab, Virginia Weis | TITLE: Symbiont density quantification in live Aiptasia
AUTHORS: Shumpei Maruyama, OSU Weis Lab, Virginia Weis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is designed for quantifying symbiont density during the onset of
symbiosis and early proliferation. Other techniques should be... | ["[Anemone preparation]\nAfter initial inoculation, re-plate anemones in a clean plate to avoid imaging algae that are not inside the host.", "[Imaging]\nRemove half of the seawater from each well.", "Add MgCl2 (3.76g MgCl2 in 50mL filtered seawater) to well for a 1:1 dilution.\nNote: start with one anemone at a time s... |
28,627 | TXTL phage production | null | dx.doi.org/10.17504/protocols.io.77thrnn | null | Marijn Ceelen | TITLE: TXTL phage production
AUTHORS: Marijn Ceelen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol explains how to use the Abor BiosciencesSigma 70 TXTL mix to produce bacteriophages. This protocol works for bacteriophage T7. It has been tested on phage lambda. However, it seems that ... | ["Calculate ratios of ingredients. TXTL master mix should always be ¾ of the reaction.Concentrations of other ingredients can be varied, but these are the tested concentrations: AB1Ingredient Concentration 2PEG-8000 3% 3GamS 3.3 µM 4dNTPs 0.5 mM The remaining part of t... |
83,844 | Rapid, high-throughput phenotypic profiling of endosymbiotic dinoflagellates (Symbiodiniaceae) using benchtop flow cytometry | 4 | dx.doi.org/10.17504/protocols.io.dm6gpjr2jgzp/v3 | https://www.protocols.io/view/rapid-high-throughput-phenotypic-profiling-of-endo-cv5cw82w | Colin J Anthony, Colin Lock, Bastian Bentlage | TITLE: Rapid, high-throughput phenotypic profiling of endosymbiotic dinoflagellates (Symbiodiniaceae) using benchtop flow cytometry
AUTHORS: Colin J Anthony, Colin Lock, Bastian Bentlage
[DESCRIPTION]
Endosymbiotic dinoflagellates (Family Symbiodiniaceae) are the primary producer of energy for many cnidarians, includi... | ["[Sample Preservation] Sample tissue from cnidarian\nIf calcifying coral, we suggest 3 pieces at around 2 cm3 sampled with wire cutters or hammer and chisel \nIf gelatinous cnidarian, 0.05 - 0.1 g of tissue sampled with sterile scissors works well", "[Sample Preservation] Store tissue in bag or tube\nFor calcifiers, W... |
5,498 | Illumina (post-MR DNA) processing pipeline : QIIME | null | dx.doi.org/10.17504/protocols.io.hk2b4ye | null | Kylie Langlois | TITLE: Illumina (post-MR DNA) processing pipeline : QIIME
AUTHORS: Kylie Langlois
[DESCRIPTION]
<p>This pipeline is for datasets generated from Illumina (2x300) sequencing by MR DNA. MR DNA runs sequences through a proprietary pipeline for quality control of sequences and OTU clustering. This pipeline begins with the ... | ["[Create a mapping file]\nIn QIIME, use the alpha rarefaction script to rarefy the OTU abundance table according to specific alpha diversity metrics. Before that, you need to create a mapping file, a parameters file, and convert the OTU table into the biom format. Create a mapping file and validate it. Create a mappin... |
null | null | null | dx.doi.org/10.17504/protocols.io.p72drqe | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
20,502 | Data for manuscript: Estimation of seed yield in oilseed rape to identify the potential of semi-resynthesized parents for the development of new hybrid cultivars | null | dx.doi.org/10.17504/protocols.io.x9wfr7e | null | Laurencja Szała, Zygmunt Kaczmarek, Wiesława Popławska, Alina Liersch, Marek Wójtowicz, Marcin Matuszczak, Zdzisław R. Biliński, Katarzyna Sosnowska, Michał Stefanowicz, Teresa Cegielska-Taras | TITLE: Data for manuscript: Estimation of seed yield in oilseed rape to identify the potential of semi-resynthesized parents for the development of new hybrid cultivars
AUTHORS: Laurencja Szała, Zygmunt Kaczmarek, Wiesława Popławska, Alina Liersch, Marek Wójtowicz, Marcin Matuszczak, Zdzisław R. Biliński, Katarzyna Sos... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.c7fzjm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Case 2: Fix samples. Use this protocol when long term storage of slide is required.<br /><br />For when you can count your samples in a few days, see <a href="https://www.protocols.io/view/SYBR-Gold-Staining-for-Viral-Enumeration-Case-1-c69zh5" target="_blank">Case 1</a>.
... | [] |
85,933 | GFP-YIPF3 Immunoprecipitation V2 | 4 | dx.doi.org/10.17504/protocols.io.81wgbxy6qlpk/v1 | https://www.protocols.io/view/gfp-yipf3-immunoprecipitation-v2-cx6mxrc6 | Kelsey Hickey, Harper JW, Sharan Sharan Swarup | TITLE: GFP-YIPF3 Immunoprecipitation V2
AUTHORS: Kelsey Hickey, Harper JW, Sharan Sharan Swarup
[DESCRIPTION]
This is a protocol for the immunoprecipitation of the Golgi protein YIPF3 that is GFP tagged, and probing for ATG8 protein interactions.
[STEPS]
SECTION: HEK293 YIPF4 KO Creation
1. Maintain HEK293 cells in ... | ["[HEK293 YIPF4 KO Creation] Maintain HEK293 cells in Dulbecco’ Modifies Eagles Medium (DMEM) with 10% fetal bovine serum and optional 1x penicillin-streptomycin.", "[HEK293 YIPF4 KO Creation] Individual clones were subjected to immunoblotting with anti-YIPF4 (Sino Biological 202844-T46), clones lacking the relevant pr... |
21,099 | E. coli K12 DNA Extraction | null | dx.doi.org/10.17504/protocols.io.yujfwun | null | Kenneth Schackart, Kattika Kaarj | TITLE: E. coli K12 DNA Extraction
AUTHORS: Kenneth Schackart, Kattika Kaarj
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>How to extract DNA from </span><span style = "font-style:italic;">E. coli</span><span> K12 using Wizard® Genomic DNA Purification Kit by Promega®.</span></div><div class ... | ["[Culture bacteria]\nCulture E. coli K12 in BHI broth overnight.lyophilized E. coli K12 in BHI broth.\n2 mg\n10 ml", "[Pellet the cells]\nAdd cell suspension to 2 mL microcentrifuge tube.\n1 ml", "[Pellet the cells]\nCentrifuge at 13,000-16,000 × g for .", "[Pellet the cells]\nRemove supernatant.", "[Lyse nuclei]\nAdd... |
null | null | null | dx.doi.org/10.17504/protocols.io.k4kcyuw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Provide a modified Bradford method to determine the residual protein content in the bio-synthesis product. </p>
<p>The product may be obtained from enzymatic reaction and extract from organic solvent, and /or insoluble in water.</p>
[GUIDELINES]
<p>May not suitable the prod... | [] |
103,187 | Rat astrocyte isolation and culture | 0 | dx.doi.org/10.17504/protocols.io.n2bvjnpxpgk5/v1 | https://www.protocols.io/view/rat-astrocyte-isolation-and-culture-dgzt3x6n | Shiyi Wang | TITLE: Rat astrocyte isolation and culture
AUTHORS: Shiyi Wang
[DESCRIPTION]
Rat astrocyte isolation and culture
[STEPS]
1. **Cortex Dissection and Digestion** - Micro-dissect P1 rat cortices from both sexes. - Digest cortices in papain solution.
2. **Trituration and Resuspension** - Triturate tissue in low and high ... | ["**Cortex Dissection and Digestion** - Micro-dissect P1 rat cortices from both sexes. - Digest cortices in papain solution.", "**Trituration and Resuspension** - Triturate tissue in low and high ovomucoid solutions. - Resuspend cells in astrocyte growth media (AGM): - DMEM (GIBCO 11960) - 10% FBS - 10 μM hydrocortison... |
72,218 | darkmode protocol | 1 | null | https://www.protocols.io/view/darkmode-protocol-cir2ud8e | Maria Gul | TITLE: darkmode protocol
AUTHORS: Maria Gul
[DESCRIPTION]
Piqued favour stairs it enable exeter as seeing. Remainder met improving but engrossed sincerity age. Better but length gay denied abroad are. Attachment astonished to on appearance imprudence so collecting in excellence. Tiled way blind lived whose new. The fo... | ["Piqued favour stairs it enable exeter as seeing. Remainder met improving but engrossed sincerity age. Better but length gay denied abroad are. Attachment astonished to on appearance imprudence so collecting in excellence. Tiled way blind lived whose new. The for fully had she there leave merit enjoy forth.\n\nBetraye... |
43,139 | Protocol 3: Antiobiotic Resistance | 4 | null | https://www.protocols.io/view/protocol-3-antiobiotic-resistance-bndbma2n | TITLE: Protocol 3: Antiobiotic Resistance
AUTHORS:
[STEPS]
?. Plate the CRISPR solution. Take LB Strep/Kan/Arabinose agar plate out of the fridge and let sit to adjust to room temperature. Shake the microcentrifuge tube containing the CRISPR experiment and then pour or pipette contents onto the agar plate. Use an i... | ["Plate the CRISPR solution. Take LB Strep/Kan/Arabinose agar plate out of the fridge and let sit to adjust to room temperature. Shake the microcentrifuge tube containing the CRISPR experiment and then pour or pipette contents onto the agar plate. Use an inoculation loop to spread contents onto the plate. Let dry for ... | |
101,635 | Protocol for Nuclei/ Cell Isolation and 10X Genomics Fixed RNA profiling for Human Ovary | 0 | dx.doi.org/10.17504/protocols.io.n92ld8rwxv5b/v1 | https://www.protocols.io/view/protocol-for-nuclei-cell-isolation-and-10x-genomic-dfhb3j2n | Nicolas Martin | TITLE: Protocol for Nuclei/ Cell Isolation and 10X Genomics Fixed RNA profiling for Human Ovary
AUTHORS: Nicolas Martin
[DESCRIPTION]
This is the 10X Genomics protocol to fix, dissociate, and profile RNA from human ovary tissue.
[GUIDELINES]
This protocol needs prior approval by the users' institutional review board... | ["[Cell/Nuclei Isolation Protocol for Human Skeletal Muscle] The protocol CG000553 REV B was used to fix, dissociate, and isolate cells/nuclei from frozen human skeletal muscle with the following modifications: \n\n1) 0.25 mg / mL of Liberase TH was used for dissociation at Step 2b, Page 6.\n2) Two extra \"spin only\" ... |
28,169 | Morphometry: Mouse | null | dx.doi.org/10.17504/protocols.io.7rhhm36 | null | Ed Leiter | TITLE: Morphometry: Mouse
AUTHORS: Ed Leiter
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This protocol describes a number of the measurements that are made on the mouse anatomy.</div><div class = "text-block"><span... | ["The left kidney weight is obtained after dissection from the abdominal cavity and the weight is obtained on a balance with 0.1 mg discrimination. The weight is obtained wet (non-dissecated) after the kidney is blotted on paper towels. This is the left kidney of the animal.", "The right kidney weight is obtained after... |
85,822 | HCR RNA-FISH protocol for the whole-mount brains of Drosophila melanogaster | 4 | null | https://www.protocols.io/view/hcr-rna-fish-protocol-for-the-whole-mount-brains-o-cx26xqhe | Amanda A. G. Ferreira, Bogdan Sieriebriennikov, Hunter Whitbeck | TITLE: HCR RNA-FISH protocol for the whole-mount brains of Drosophila melanogaster
AUTHORS: Amanda A. G. Ferreira, Bogdan Sieriebriennikov, Hunter Whitbeck
[DESCRIPTION]
This is a protocol to perform RNA fluorescent in situ hybridization (RNA-FISH) using hybridization chain reaction (HCR) on whole-mount samples of the... | ["[Day 1] Prepare all solutions", "[Day 1] Pre-heat an aliquot of Probe Hybridization Buffer if proceeding with hybridization on the same day (see step case below)\n250 µL (If preparing a new probe mixture)\n100 µL (If reusing probes)\n37 °C", "[Day 1] Dissect brains in cold Schneider's medium", "[Day 1] Fix brains in... |
33,854 | Total Flavonoid Content (TFC) | null | dx.doi.org/10.17504/protocols.io.bda6i2he | null | Jing Xu | TITLE: Total Flavonoid Content (TFC)
AUTHORS: Jing Xu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The amounts of total flavonoids was quantified. The reaction mixture consisted of 1.0 ml of extract, 0.3 ml of 5% sodium nitrite and 4 ml of 60% ethanol. After 6 min, 0.3 ml of 10% aluminium nitrite... | [] |
106,752 | Isolation of lysosomes using the Tagless LysoIP method in PBMCs | 4 | dx.doi.org/10.17504/protocols.io.x54v9yp51g3e/v3 | https://www.protocols.io/view/isolation-of-lysosomes-using-the-tagless-lysoip-me-dkg84tzw | Daniel Saarela, Esther Sammler, Dario R Alessi, Francesca Tonelli | TITLE: Isolation of lysosomes using the Tagless LysoIP method in PBMCs
AUTHORS: Daniel Saarela, Esther Sammler, Dario R Alessi, Francesca Tonelli
[DESCRIPTION]
Molecular homeostasis in cells is regulated in part by protein degradation, which is facilitated by the proteasome and lysosomal proteolysis. Lysosomes are mem... | ["[Methods] Prepare a 0.5 Molarity (M) stock solution of DIFP by diluting in isopropanol under a fume hood. Stock solution can be stored in -80 °C until needed.", "[Methods] Collect blood into a BD Vacutainer sodium heparin 10-mL tubes.", "[Methods] Add density gradient medium to the SepMate tube using a 20-mL syringe ... |
106,808 | Tardigrade videography, tracking, and step analysis | 0 | dx.doi.org/10.17504/protocols.io.kxygxy8nwl8j/v1 | https://www.protocols.io/view/tardigrade-videography-tracking-and-step-analysis-dkiy4ufw | Ian Woods | TITLE: Tardigrade videography, tracking, and step analysis
AUTHORS: Ian Woods
[DESCRIPTION]
Protocols for tardigrade handling, preparation for videography, video acquisition, path tracking, step timing quantification, and analysis. Descriptions of Python scripts used in the analysis pipeline.
[STEPS]
SECTION: Gel-bot... | ["[Gel-bottomed dishes] Tardigrades will not generally walk in glass or plastic Petri dishes, because they need something to grip with their claws. Hypsibius exemplaris individuals reliably walk if they are able to grasp the surface with all eight claws. We use plastic Petri dishes lined with 2% agar or agarose (in Pol... |
85,185 | Environmental DNA (eDNA) COI PCR Amplification and Gel Electrophoresis Protocol | 1 | dx.doi.org/10.17504/protocols.io.dm6gp3wpdvzp/v1 | https://www.protocols.io/view/environmental-dna-edna-coi-pcr-amplification-and-g-cxe9xjh6 | Meghan M. Shea, Alexandria B Boehm | TITLE: Environmental DNA (eDNA) COI PCR Amplification and Gel Electrophoresis Protocol
AUTHORS: Meghan M. Shea, Alexandria B Boehm
[DESCRIPTION]
This is a protocol for amplifying DNA extracts via PCR using broad COI primers (Leray et al., 2013) and confirming successful amplification via gel electrophoresis.
This pr... | ["[Set-Up] Wipe down 10 µL pipette with 70% ethanol and RNase Away. UV for 10 minutes on each side", "[Set-Up] Wipe down a staging area near the PCR hood with 10% bleach, 70% ethanol, and RNase Away", "[Set-Up] Wipe down ice bin with 10% bleach, 70% ethanol, and RNase away, fill with ice, and remove DNA samples, PCR-gr... |
31,405 | Cloning shRNA Oligos into pLKO.1 | null | dx.doi.org/10.17504/protocols.io.bawmifc6 | null | Addgene The Nonprofit Plasmid Repository | TITLE: Cloning shRNA Oligos into pLKO.1
AUTHORS: Addgene The Nonprofit Plasmid Repository
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is the protocol accompanying the "pLKO.1 – TRC Cloning Vector". For information about the PLKO.1-TRC cloning vector and tips on designing shRNA oligos for pL... | ["[Annealing Oligos]\nResuspend oligos in ddH2O to a concentration of 20 μM.", "[Annealing Oligos]\nAdd 5ul Forward oligo\n5 µl", "[Annealing Oligos]\nAdd 5ul Reverse oligo\n5 µl", "[Annealing Oligos]\nAdd 5 μL\t10x NEB buffer 2\n5 µl", "[Annealing Oligos]\nAdd 35 μL ddH2O\n35 µl", "[Annealing Oligos]\nIncubate for 4 m... |
49,211 | Single cell RNA Sequencing for tissue samples processed with microfluidic device platform | 1 | dx.doi.org/10.17504/protocols.io.bua3nsgn | https://www.protocols.io/view/single-cell-rna-sequencing-for-tissue-samples-proc-bua3nsgn | Jeremy Lombardo | TITLE: Single cell RNA Sequencing for tissue samples processed with microfluidic device platform
AUTHORS: Jeremy Lombardo
[DESCRIPTION]
Protocol for single cell RNA sequencing of tissue samples processed with a microfluidic device platform.
[STEPS]
1. Euthanize mice (12 week, male, C57BL/6 for kidney; 10 week, femal... | ["Euthanize mice (12 week, male, C57BL/6 for kidney; 10 week, female, MMTV-PyMT for mammary tumor, both from Jackson Laboratory, Bar Harbor, ME) by CO2 inhalation.", "Dissect kidneys or mammary tumor and mince into ~1 mm3 pieces.", "Process samples using microfluidic device platform.", "After device processing, centri... |
43,120 | 5 Methods for DNA-protein imaging by AFM in fluid | 1 | dx.doi.org/10.17504/protocols.io.bncqmavw | https://www.protocols.io/view/5-methods-for-dna-protein-imaging-by-afm-in-fluid-bncqmavw | Philip J. Haynes, Kavit H. S. Main, Alice Pyne | TITLE: 5 Methods for DNA-protein imaging by AFM in fluid
AUTHORS: Philip J. Haynes, Kavit H. S. Main, Alice Pyne
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is part 5 of the "Atomic Force Microscopy of DNA and DNA-Protein Interactions" collection of protocols.</div><div class = "text-block"... | ["[Methods for DNA-protein imaging by AFM in fluid]\nPrepare a mica substrate as described in protocol 1.", "[Methods for DNA-protein imaging by AFM in fluid]\nPEGylate the substrate and adsorb DNA/DNA-protein as described in protocol 2, method 2.3. Place the sample in the AFM.", "[Methods for DNA-protein imaging by AF... |
null | null | null | dx.doi.org/10.17504/protocols.io.usxewfn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
de novo sequencing mass spectrometry is an very important process where amino acid sequences can be interpreted from tandem mass spectra without the assistance of a database.
also Peptidomics analysis studies can help yoou gain a better understanding of information about diseas... | ["{\"blocks\":[{\"key\":\"ep3bo\",\"text\":\" \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[{\"offset\":0,\"length\":1,\"key\":0}],\"data\":[]},{\"key\":\"7fv11\",\"text\":\"\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]}],\"entityMap\":[{... |
34,714 | DNA extraction from environmental biofilm using the NucleoSpin® Soil kit (MACHEREY-NAGEL) | null | dx.doi.org/10.17504/protocols.io.bd52i88e | null | Marine Vautier, Valentin Vasselon, Cecile Chardon, Frédéric Rimet, Agnès Bouchez, Isabelle Domaizon | TITLE: DNA extraction from environmental biofilm using the NucleoSpin® Soil kit (MACHEREY-NAGEL)
AUTHORS: Marine Vautier, Valentin Vasselon, Cecile Chardon, Frédéric Rimet, Agnès Bouchez, Isabelle Domaizon
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:le... | ["[Prepare the sample]\nShake the 50mL falcon tube containing the biofilm / ethanol mixture by inverting the tube in order to obtain a homogeneous solutionTake 2 mL of this homogeneized solution by pipetting twice 1 mL into a 2 mL sterile microcentrifuge tube and close the capNote : between each pipetting, aspirate/dis... |
31,816 | Making LB NGM Plates | null | dx.doi.org/10.17504/protocols.io.bbbgiijw | null | Priota Islam | TITLE: Making LB NGM Plates
AUTHORS: Priota Islam
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">C. elegans is maintained in the laboratory on Nematode Growth Medium (NGM) agar which has been aseptically poured into petri plates. Sometimes they can also be grown on LB-NGM, where the LB in the media... | ["[Pre-Autoclave:]\nBook the autoclave (notebook on top of the machine).", "[Pre-Autoclave:]\nTake clean flasks from the glass kitchen (Only the ones with autoclave tape on are sterile)", "[Pre-Autoclave:]\nMeasure all the pre-autoclave reagents and add to the flask (Use a new weighing boat and spatula for each reagent... |
50,871 | CELL STORAGE-02-Freezing and Thawing Protocol for Suspension Cell Lines | 4 | dx.doi.org/10.17504/protocols.io.bvwxn7fn | https://www.protocols.io/view/cell-storage-02-freezing-and-thawing-protocol-for-bvwxn7fn | Marco Cosentino, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Mariagiulia Albizzati, Marco Ferrari, Franca Marino, Alessandra Luini, Alessandra Luini | TITLE: CELL STORAGE-02-Freezing and Thawing Protocol for Suspension Cell Lines
AUTHORS: Marco Cosentino, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Mariagiulia Albizzati, Marco Ferrari, Franca Marino, Alessandra Luini, Alessandra Luini
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><d... | ["[CELL FREEZING PROCEDURE FOR SUSPENSION CELLS (PC-12)]\nAt confluence remove cell suspension from the T25 cm2 culture flask and recover in 15 mL conical tube.", "[CELL FREEZING PROCEDURE FOR SUSPENSION CELLS (PC-12)]\nCentrifuge for at .\nCentrifuge: 200 34\n0 Room temperature", "[CELL FREEZING PROCEDURE FOR SUSPENS... |
62,273 | Power Keto Gummy - *SCAMING ALERT*! You Won’t Believe Power Keto Gummies Report! | 3 | dx.doi.org/10.17504/protocols.io.j8nlkkne1l5r/v1 | https://www.protocols.io/view/power-keto-gummy-scaming-alert-you-won-t-believe-p-b829ryh6 | G A | TITLE: Power Keto Gummy - *SCAMING ALERT*! You Won’t Believe Power Keto Gummies Report!
AUTHORS: G A
[DESCRIPTION]
However, there are still ways that consumers can afford health coverage despite increasing premiums.
[STEPS] | [] |
11,640 | Isolation of mitochondria from Diplonema papillatum | null | dx.doi.org/10.17504/protocols.io.pkydkxw | null | Matus Valach | TITLE: Isolation of mitochondria from Diplonema papillatum
AUTHORS: Matus Valach
[STEPS]
?. Inoculate 50 mL of OSS growth medium supplemented with yeast extract (0.05%) and chloramphenicol (40 mg/L) with an axenic stock of Diplonema cells. Cultivate at 16 °C with occasional shaking (e.g. 4× per day) to cell density ap... | ["Inoculate 50 mL of OSS growth medium supplemented with yeast extract (0.05%) and chloramphenicol (40 mg/L) with an axenic stock of Diplonema cells. Cultivate at 16 °C with occasional shaking (e.g. 4× per day) to cell density approx. 5×106 – 107 per mL. This corresponds approximately to the late exponential phase and ... |
81,398 | Soil sample DNA extraction (SuperSoil) | 4 | dx.doi.org/10.17504/protocols.io.rm7vzbzj2vx1/v1 | https://www.protocols.click/view/soil-sample-dna-extraction-supersoil-ctqwwmxe | Hsin-Mao Wu, Jie Hao Ou, Yin-Tse Huang, Yu-Hsuan Fan | TITLE: Soil sample DNA extraction (SuperSoil)
AUTHORS: Hsin-Mao Wu, Jie Hao Ou, Yin-Tse Huang, Yu-Hsuan Fan
[DESCRIPTION]
A method for fungal and bacterial DNA extraction.
[STEPS]
SECTION: Sample preparation & Cell lysis
1. Add 0.2 mL ⌀=1.0 mm zirconia beads, 0.2 mL ⌀=0.5 mm zirconia beads, 800 µL SD1 solution... | ["[Sample preparation & Cell lysis] Add 0.2 mL ⌀=1.0 mm zirconia beads, 0.2 mL ⌀=0.5 mm zirconia beads, 800 µL SD1 solution, 0.5 g into a 1.5 ml microcentrifuge tube. Vortex briefly to mix.", "[Sample preparation & Cell lysis] Homogenize samples using Vortex-Genie® 2 with SI-H524 Horizontal microtube hold... |
null | null | null | dx.doi.org/10.17504/protocols.io.eyibfue | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Mapping metagenomic reads from <a href="https://github.com/MicroB3-IS/osd-analysis" target="_blank">Ocean Sampling Da</a>y (OSD) 2014 against NCBI's ViralRefSeq alongside viral sequences identified from the Tara Oceans survey using <a href="http://www.ncbi.nlm.nih.gov/pmc/articl... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ks8cwhw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Is an RNA loading dye. </p>
[STEPS]
?.
?. | [] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.