id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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88,546 | A method to prepare Sera-Mag SpeedBeads for purification and size selection of nucleic acids | 4 | dx.doi.org/10.17504/protocols.io.x54v9p7b1g3e/v1 | https://www.protocols.io/view/a-method-to-prepare-sera-mag-speedbeads-for-purifi-c2qaydse | John B. Ridenour, Rafal Donczew | TITLE: A method to prepare Sera-Mag SpeedBeads for purification and size selection of nucleic acids
AUTHORS: John B. Ridenour, Rafal Donczew
[DESCRIPTION]
In this protocol, we describe a method to prepare Sera-Mag Speedbeads for purification and size selection of nucleic acids. We additionally describe a method to va... | ["[Preparation of 10% (v/v) Tween 20] Place a 50 ml conical tube on a balance and tare the balance.", "[Preparation of 10% (v/v) Tween 20] Slowly add 5.475 g of Tween 20 to the conical tube using a serological pipette.", "[Preparation of 10% (v/v) Tween 20] Remove the conical tube from the balance and add 45 ml of Mill... |
72,296 | Washington University SenNet Case Collection Protocol | 1 | dx.doi.org/10.17504/protocols.io.bp2l699rdlqe/v1 | https://www.protocols.io/view/washington-university-sennet-case-collection-proto-ciuguetw | Daniel Rapp, Matthew Wyczalkowski | TITLE: Washington University SenNet Case Collection Protocol
AUTHORS: Daniel Rapp, Matthew Wyczalkowski
[DESCRIPTION]
This document outlines inclusion and exclusion criteria for healthy participants for the WUSTL Senescence Network (SenNet) by the Tissue Mapping Center (TMC) at Washington University.
[STEPS]
1. Stud... | ["Study coordinator will receive surgeon schedules at the beginning of each week. All surgeons should be IRB approved for the study.", "Study coordinator will evaluate patient for eligibility using criteria detailed in following sections.", "Study coordinator will call potential study participants over phone using IRB ... |
74,687 | Isotope sample preparation of diatoms for paleoenvironmental research | 1 | dx.doi.org/10.17504/protocols.io.36wgq4knovk5/v2 | https://www.protocols.io/view/isotope-sample-preparation-of-diatoms-for-paleoenv-ck67uzhn | George Swann, Andreasnelling | TITLE: Isotope sample preparation of diatoms for paleoenvironmental research
AUTHORS: George Swann, Andreasnelling
[DESCRIPTION]
Isotopes in diatoms are increasingly used in palaeoenvironmental studies in both lacustrine and marine settings, enabling the reconstruction of a range of variables including temperature, pr... | ["[Disaggregation of samples] This step breaks up aggregated sediment using non-alkaline chemicals so that diatoms can be successfully extracted. Full removal of external organic matter occur later in Step 6 of the protocol.", "[Disaggregation of samples] Place up to 1 cm3 of freeze dried sediment sample in 10-12 ml ce... |
65,248 | ProDentim Reviews Scam exposed 2022 And where to buy | 3 | dx.doi.org/10.17504/protocols.io.bp2l61exzvqe/v1 | https://www.protocols.io/view/prodentim-reviews-scam-exposed-2022-and-where-to-b-cbx8sprw | prodentim | TITLE: ProDentim Reviews Scam exposed 2022 And where to buy
AUTHORS: prodentim
[DESCRIPTION]
The ProDentim is a natural dietary supplement that supports healthy gums and strong teeth as well as provides a safe and all-natural treatment for a variety of dental conditions. ProDentim pill produces calming effects by co... | [] |
38,081 | Metabarcoding using MinION: PCR, Multiplexing and Library Preparation | 1 | dx.doi.org/10.17504/protocols.io.bhe9j3h6 | https://www.protocols.io/view/metabarcoding-using-minion-pcr-multiplexing-and-li-bhe9j3h6 | Bastian Egeter, Joana Veríssimo, Manuel Lopes-Lima, Cátia Chaves, Joana Pinto, Nicoletta Riccardi, Pedro Beja, Nuno A. Fonseca | TITLE: Metabarcoding using MinION: PCR, Multiplexing and Library Preparation
AUTHORS: Bastian Egeter, Joana Veríssimo, Manuel Lopes-Lima, Cátia Chaves, Joana Pinto, Nicoletta Riccardi, Pedro Beja, Nuno A. Fonseca
[DESCRIPTION]
A protocol for the metabarcoding of DNA samples using nanopore technology, for the purposes ... | ["[Preparing first PCR] Prepare first PCR with a 25 µL total PCR volume using optimised conditions for the primers / enzyme in use. Primers should include MinION adapters (5´-TTTCTGTTGGTGCTGATATTGC-forward primer-3´, 5´-ACTTGCCTGTCGCTCTATCTTC-reverse primer-3´).", "[Preparing first PCR] Test PCR product to assess ampli... |
47,855 | SARS-CoV-2 NCBI submission workflow + guidance for structuring and releasing metadata | 2 | dx.doi.org/10.17504/protocols.io.bsypnfvn | https://www.protocols.io/view/sars-cov-2-ncbi-submission-workflow-guidance-for-s-bsypnfvn | Ruth Timme, Emma Griffiths | TITLE: SARS-CoV-2 NCBI submission workflow + guidance for structuring and releasing metadata
AUTHORS: Ruth Timme, Emma Griffiths
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">PURPOSE: </span></div><div class = "text-block"><span style = "font-weight:bold;">This wo... | [] |
91,143 | eDNA Water Sample Collection, Preservation and Extraction (low-tech sampling, modified Qiagen PowerWater extraction) | 4 | dx.doi.org/10.17504/protocols.io.4r3l22b7pl1y/v1 | https://www.protocols.io/view/edna-water-sample-collection-preservation-and-extr-c49fyz3n | Eldridge Wisely | TITLE: eDNA Water Sample Collection, Preservation and Extraction (low-tech sampling, modified Qiagen PowerWater extraction)
AUTHORS: Eldridge Wisely
[DESCRIPTION]
Marine environmental DNA (eDNA) collection, filtration, preservation, and extraction protocol used in Galápagos fieldwork 2021-2023, and Gulf of California ... | ["[Water collection and filtration] Collect water samples in a cleaned (see step 12) 1L Nalgene bottle by submerging the bottle and then opening it and closing it underwater. This step can be adapted to your sampling logistics, while reducing as much as possible any contact between your skin or clothes or other equipme... |
21,844 | Enzo’s CGH Labeling Kit for Oligo Arrays | null | dx.doi.org/10.17504/protocols.io.zjuf4nw | null | Hendrik F van Essen | TITLE: Enzo’s CGH Labeling Kit for Oligo Arrays
AUTHORS: Hendrik F van Essen
[STEPS]
?. [Denature DNA and anneal random primers]
pipette the following in a nuclease free thermocycler strip:a. DNA X µlb. water 19 – X µl (Vial W) b. Primers/Reaction buffer 20 µl (Vial 1) Total volume: 39 µl
?. [Denature DNA ... | ["[Denature DNA and anneal random primers]\npipette the following in a nuclease free thermocycler strip:a.\tDNA\t X µlb. water\t19 – X µl\t(Vial W) b.\tPrimers/Reaction buffer\t20 µl\t(Vial 1) Total volume:\t39 µl", "[Denature DNA and anneal random primers]\nAdd cap, flick to mix contents and briefly spin do... |
85,309 | DNA quantification, Purity and Integrity | 4 | dx.doi.org/10.17504/protocols.io.81wgbx6pqlpk/v1 | https://www.protocols.click/view/dna-quantification-purity-and-integrity-cxi5xkg6 | Elena L. Peredo | TITLE: DNA quantification, Purity and Integrity
AUTHORS: Elena L. Peredo
[DESCRIPTION]
We will use three complementary methods to asses DNA.
[STEPS]
SECTION: Quantification.
1. Qubit system https://www.thermofisher.com/order/catalog/product/Q32850#/Q32850
This protocol assumes that you are preparing standards for ca... | ["[Quantification.] Qubit system https://www.thermofisher.com/order/catalog/product/Q32850#/Q32850 \nThis protocol assumes that you are preparing standards for calibrating the Qubit® Fluorometer. If you plan to use the last calibration performed on the instrument (see “Qubit® Fluorometer calibration” on page 2), you ne... |
46,076 | Construction of a Moore Swab | 1 | null | https://www.protocols.io/view/construction-of-a-moore-swab-bq84mzyw | Dilip Abraham, Nicola Elviss, Nicholas Feasey, Nick Grassly, Jacob John, Gagandeep Kang, John Scott Meschke, Venkata Raghava Mohan, Satheesh Nair, Jonathan Rigby, Rajan Srinivasan, Catherine Troman, Christopher Uzzell, Nicolette Zhou | TITLE: Construction of a Moore Swab
AUTHORS: Dilip Abraham, Nicola Elviss, Nicholas Feasey, Nick Grassly, Jacob John, Gagandeep Kang, John Scott Meschke, Venkata Raghava Mohan, Satheesh Nair, Jonathan Rigby, Rajan Srinivasan, Catherine Troman, Christopher Uzzell, Nicolette Zhou
[DESCRIPTION]
Moore swabs are gau... | ["Fold the gauze eight times length-wise to form an 8-ply square", "Tie the gauze around the middle with twine/fishing line leaving a long tail for attaching the swab once it has been placed."] |
56,328 | Cleaning, Aggregating, and Filtering CMU Libraries Open Science and Data Collaborations Program Data | 4 | dx.doi.org/10.17504/protocols.io.b29gqh3w | https://www.protocols.io/view/cleaning-aggregating-and-filtering-cmu-libraries-o-b29gqh3w | Patrick Campbell, Huajin Wang, Melanie Gainey, Sarah Young, Katie Behrman | TITLE: Cleaning, Aggregating, and Filtering CMU Libraries Open Science and Data Collaborations Program Data
AUTHORS: Patrick Campbell, Huajin Wang, Melanie Gainey, Sarah Young, Katie Behrman
[DESCRIPTION]
This document describes the process and tools used to clean, aggregate, and filter the data resources collected... | ["[Designing Final Data Model] Each row in the final master dataset represents a unique user and columns represent attributes related to their identity and activity. The data is organized using the data model pictured below (Figure 1).", "[Cleaning] First, import all the component datasets (OSF, Workshops, LabArchives,... |
null | null | null | dx.doi.org/10.17504/protocols.io.phjdj4n | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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48,468 | PLF Plating and Freezing Protocol | 1 | null | https://www.protocols.io/view/plf-plating-and-freezing-protocol-btjunknw | Morrisey Lab | TITLE: PLF Plating and Freezing Protocol
AUTHORS: Morrisey Lab
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.d2y8fv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
In a study comparing obese versus lean twins, Turnbaugh and colleagues found that there were "shared microbial genes among sampled individuals, comprising an extensive, identifiable 'core microbiome' at the gene, rather than at the organismal lineage level". <br />This means th... | [] |
105,999 | Determination of Lethal and Effective Concentrations (LCx and ECx) of Hydrophobic Organic Contaminants in Parhyale hawaiensis | 0 | dx.doi.org/10.17504/protocols.io.14egn6rbyl5d/v1 | https://www.protocols.io/view/determination-of-lethal-and-effective-concentratio-djrp4m5n | Ibrahim Lawan | TITLE: Determination of Lethal and Effective Concentrations (LCx and ECx) of Hydrophobic Organic Contaminants in Parhyale hawaiensis
AUTHORS: Ibrahim Lawan
[DESCRIPTION]
This protocol outlines a robust methodology for assessing the acute toxicity of selected Polycyclic Aromatic Hydrocarbons (PAHs) in the tropical mari... | ["[INTRODUCTION] Polycyclic Aromatic Hydrocarbons (PAHs) are a critical class of hydrophobic organic contaminants known for their persistence, bioaccumulation potential, and toxicity within marine ecosystems (Honda & Suzuki, 2020). With the increasing prevalence of PAHs due to anthropogenic activities, it is essential ... |
96,396 | Exp01_protocolo | 0 | dx.doi.org/10.17504/protocols.io.bp2l6xodrlqe/v1 | https://www.protocols.io/view/exp01-protocolo-dadk2a4w | Julio Cesar Quintero Gámez | TITLE: Exp01_protocolo
AUTHORS: Julio Cesar Quintero Gámez
[DESCRIPTION]
Nuestro experimento consistió en investigar el comportamiento en la temperatura de una taza a 68 °C expuesta a una temperatura ambiente de 19 °C. Utilizamos sensores DS18B20 para medir tanto la temperatura del agua como la del entorno. Los resul... | ["[Código y Arduino] Instalación del software a utilizar.", "[Código y Arduino] Armado del circuito", "[Experimento] Vierte 100 mL de agua en tu olla. Enciende la parrilla y toma mediciones cada cierto tiempo para saber cuando el agua llegue a 68 °C para verterlo a la taza.", "[Experimento] Introduce uno de los sensore... |
41,148 | Pooling protocol using AMPHABIO HT-HiThroughput PCR COVID-19 Kit | 4 | dx.doi.org/10.17504/protocols.io.bke4ktgw | https://www.protocols.io/view/pooling-protocol-using-amphabio-ht-hithroughput-pc-bke4ktgw | hohuutho | TITLE: Pooling protocol using AMPHABIO HT-HiThroughput PCR COVID-19 Kit
AUTHORS: hohuutho
[STEPS]
?. [Pooling protocol]
Pre-amplification
?. [Pooling protocol]
Master mix preparation- Thaw all reagents to obtain homogeneous solutions. Mix all the tubes gently with the vortex mixer and briefly spin down. Do not leave ... | ["[Pooling protocol]\nPre-amplification", "[Pooling protocol]\nMaster mix preparation- Thaw all reagents to obtain homogeneous solutions. Mix all the tubes gently with the vortex mixer and briefly spin down. Do not leave the reagents at room temperature for more than 30 minutes.- Keep all the tubes on ice.- Prepare the... |
null | null | null | dx.doi.org/10.17504/protocols.io.f29bqh6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Experiment purpose: measure dynamics of phage infection and gene expression in cyanobacteria. We collect samples every 1-2 hours over a 12 hour period, starting from an initial infection volume of 200ml per replicate per treatment.</p>
[STEPS]
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33,444 | Flongle DirectRNA Library preparation | null | dx.doi.org/10.17504/protocols.io.bcwcixaw | null | Maximilian Krause | TITLE: Flongle DirectRNA Library preparation
AUTHORS: Maximilian Krause
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Oxford Nanopore Technologies allows sequencing of native RNA for the first time. Additionally they released tiny devices that democratize sequencing among scientists. However, the ... | ["[Flongle native RNA library preparation (SQK-RNA002)]\nTake poly(A)-selected RNA into a 0.2ml thin-walled DNA-free PCR tube and bring volume to with RNase-free water\n200 ng\n9.5 µl\nThe following description of Nanopore Library preparation is based on the protocols and consumable recommendations available at the d... |
96,160 | Making tetracycline LB agar plates | 0 | dx.doi.org/10.17504/protocols.io.yxmvm388bl3p/v1 | https://www.protocols.io/view/making-tetracycline-lb-agar-plates-c958z89w | Bonnie Evans | TITLE: Making tetracycline LB agar plates
AUTHORS: Bonnie Evans
[DESCRIPTION]
Making LB agar plates containing tetracycline for selecting strains carrying the pJC8 cosmid. Plates can be stored at 4 °C wrapped in aluminium foil for up to a month.
[STEPS]
SECTION: Making tetracycline LB agar plates
1. Autoclave or mic... | ["[Making tetracycline LB agar plates] Autoclave or microwave LB agar from media room", "[Making tetracycline LB agar plates] Make up 15 mg/ml tetracycline hydrochloride in sterile water", "[Making tetracycline LB agar plates] Let LB agar cool to 55 °C", "[Making tetracycline LB agar plates] Dilute tetracycline hydroch... |
69,796 | QIAGEN® DNeasy® PowerSoil® Pro | 4 | dx.doi.org/10.17504/protocols.io.bp2l69411lqe/v1 | https://www.protocols.io/view/qiagen-dneasy-powersoil-pro-cgecttaw | Hsin-Mao Wu | TITLE: QIAGEN® DNeasy® PowerSoil® Pro
AUTHORS: Hsin-Mao Wu
[DESCRIPTION]
For the isolation of microbial genomic DNA from all soil types, including difficult samples such as compost, sediment, and manure.
[BEFORE_START]
Ensure that the PowerBead Pro Tubes rotate freely in the centrifuge without rubbing.
If Solution C... | ["[Prepare sample & Cell lysis] Spin the PowerBead Pro Tube briefly to ensure that the beads have settled at the bottom. \nAdd up to 250 mg of soil and 800 µL of Solution CD1. Vortex briefly to mix.", "[Prepare sample & Cell lysis] Homogenize samples thoroughly using one of the following methods:", "[Inhibito... |
82,864 | Retina and RPE/Choroid RNA Extraction Protocol | 4 | dx.doi.org/10.17504/protocols.io.5qpvorjzdv4o/v1 | https://www.protocols.click/view/retina-and-rpe-choroid-rna-extraction-protocol-cu6qwzdw | Chottiwatt Jittprasong | TITLE: Retina and RPE/Choroid RNA Extraction Protocol
AUTHORS: Chottiwatt Jittprasong
[DESCRIPTION]
This protocol involves the extraction of RNA from retinal and retinal pigment epithelium (RPE) cells in the subject's retina, utilizing TRIzol as the main reagent for cell lysis.
[BEFORE_START]
Prior to the initiation ... | ["[RNA Extraction] Precool the centrifuge to 4 °C", "[RNA Extraction] Add 1000 µL of TRIzol per retina and pipette up and down 30 times to homogenize the mixture.", "[RNA Extraction] Incubate for 5 min on ice", "[RNA Extraction] Add 200 µL of Chloroform per 1000 µL of TRIzol used for lysis and mix thoroughly.", "[RNA ... |
64,245 | ONT Flongle Flowcell Loading with Q20+ Chemistry | 4 | null | https://www.protocols.io/view/ont-flongle-flowcell-loading-with-q20-chemistry-cayvsfw6 | Stephen Douglas Russell | TITLE: ONT Flongle Flowcell Loading with Q20+ Chemistry
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
Overview: This protocol describes the steps used to load a Flongle flowcell utilizing the Q20+ Ligation Sequencing Kit from ONT.
Time required: 10 minutes
[STEPS]
SECTION: Prepare the loading solution
4. Tha... | ["[Prepare the loading solution] Thaw the Sequencing Buffer II (SBII), Loading Beads (LBII), Flush Buffer (FB), and Flush Tether (FLT) at room temperature. \n\nFLT is found in the \nAll of the remainder are in the \n\nOriginal full Ligation Sequencing Kit (Q20+) protocol can be found here:", "[Prepare the loading s... |
33,949 | PCR with Taq DNA Polymerase with Standard Taq Buffer(M0273) | 1 | dx.doi.org/10.17504/protocols.io.bdd5i286 | https://www.protocols.io/view/pcr-with-taq-dna-polymerase-with-standard-taq-buff-bdd5i286 | New England Biolabs | TITLE: PCR with Taq DNA Polymerase with Standard Taq Buffer(M0273)
AUTHORS: New England Biolabs
[DESCRIPTION]
This Protocol explains methods for Taq DNA Polymerase with Standard Taq Buffer (M0273).
[BEFORE_START]
Annealing temperatures should be determined using the NEB Annealing Temp Calculator.
[GUIDELINES]
OVE... | ["Set up the following reaction on ice.\n Component 25 μl reaction 50 μl reaction Final Concentration 10X Standard Taq Reaction Buffer 2.5 μl 5 μl 1X 10 mM dNTPs 0.5 µl 1 μl 200 µM 10 µM Forward Primer 0.5 µl 1 μl 0.2 µM (0.05–1 µM) 10 µM Reverse Primer 0.5 µl 1 μl 0.2 µM (0.05–1 µM) Template DNA variable v... |
103,198 | Assessing Astrocyte Territory Volume and 3D Sholl Analysis | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj734lx1/v1 | https://www.protocols.io/view/assessing-astrocyte-territory-volume-and-3d-sholl-dgz63x9e | Shiyi Wang | TITLE: Assessing Astrocyte Territory Volume and 3D Sholl Analysis
AUTHORS: Shiyi Wang
[DESCRIPTION]
Assessing Astrocyte Territory Volume and 3D Sholl Analysis
[STEPS]
1. **Section Collection**
1.1. - Collect 100 μm-thick floating sections containing the anterior cingulate cortex (ACC) and primary motor cortex (MOp) f... | ["**Section Collection**", "- Collect 100 μm-thick floating sections containing the anterior cingulate cortex (ACC) and primary motor cortex (MOp) from mice.", "- Ensure astrocytes are sparsely labeled via PALE with mCherry-CAAX.", "**Imaging**", "- Use an Olympus FV 3000 microscope with a 60x objective to acquire high... |
72,295 | Immunocytochemistry | 4 | dx.doi.org/10.17504/protocols.io.eq2ly779wlx9/v1 | https://www.protocols.io/view/immunocytochemistry-ciufuetn | Addgene The Nonprofit Plasmid Repository | TITLE: Immunocytochemistry
AUTHORS: Addgene The Nonprofit Plasmid Repository
[DESCRIPTION]
Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe the basic steps for fixing and labeling cells in culture with a primary antibody against a target protein and a fluorescent se... | ["[Seeding cells] Place a sterile poly-D-lysine coated coverslip in each well of a 24-well cell culture treated plate.", "[Seeding cells] Seed 5*10^3 HeLa cells per well.", "[Seeding cells] Allow the HeLa cells to grow to the desired density before labeling.", "[Fixing and permeabilizing cells] Gently aspirate the medi... |
null | null | null | dx.doi.org/10.17504/protocols.io.etdbei6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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78,874 | ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding | 4 | dx.doi.org/10.17504/protocols.io.dm6gpb5zdlzp/v5 | https://www.protocols.io/view/ont-v14-nanopore-adapter-ligation-for-fungal-dna-b-cq92vz8e | Stephen Douglas Russell | TITLE: ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
This process will take your A-tailed library and add the nanopore adapters. Simply combine several chemicals for a single reaction and do a bead cleanup.
Tested with:
Flowcells: Flongle 10.4.1 or Min... | ["[Adapter Ligation] Spin down the Ligation Adapter (LA) and Quick T4 Ligase, and place on ice.\n\nLA - \nQuick T4 Ligase -", "[Adapter Ligation] Thaw Ligation Buffer (LNB) at room temperature, spin down and mix by pipetting. Due to viscosity, vortexing this buffer is ineffective. Place on ice immediately after thawi... |
55,940 | Hemocytometer use to quantify concentracion of cells' suspensions | 1 | null | https://www.protocols.io/view/hemocytometer-use-to-quantify-concentracion-of-cel-b2vcqe2w | abotero | TITLE: Hemocytometer use to quantify concentracion of cells' suspensions
AUTHORS: abotero
[DESCRIPTION]
This protocol presents the procedures to estimate the concentration of cells' suspensions using an hemocytometer
[GUIDELINES]
The lower limit for accurate counting of cells in a hemocytometer is usually consi... | ["Wash the hemocytometer with a washer bottle and dry it using a paper towel", "Cover the hemocytometer with the coverslip and place it on a flat surface", "Vortex briefly the cell suspension whose concentration will be calculated", "Using a micropipette collect 10-100 µl of the previously agitated suspension", "Place ... |
27,714 | MojoSort™ Isolation Kits Column Protocol - 1 | null | dx.doi.org/10.17504/protocols.io.7bahiie | null | Sam Li | TITLE: MojoSort™ Isolation Kits Column Protocol - 1
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple pro... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes. Kits for human samples have been optimized for PBMCs, please prepare the cells using a suitable method.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) ... |
10,124 | General fungi ITS2 (ITS4ngsUni - fITS7) for Illumina amplicon sequencing | 1 | dx.doi.org/10.17504/protocols.io.bp2l6em5gqe5/v1 | https://www.protocols.io/view/general-fungi-its2-its4ngsuni-fits7-for-illumina-a-m5kc84w | Roey Angel, Eva Petrova | TITLE: General fungi ITS2 (ITS4ngsUni - fITS7) for Illumina amplicon sequencing
AUTHORS: Roey Angel, Eva Petrova
[DESCRIPTION]
A general assay for the preparation of PCR amplicons for further analysis of fungal communities by Illumina amplicon sequencing.
The primers target the 2nd internal transcribed spacer region... | ["[PCR reaction] Prepare the following master mixture on ice .\nDo not forget to prepare some additional mixture for the negative (NTC = no template) and positive controls, and to account for pipetting errors. Work in a clean PCR box.", "[PCR program] Run the following PCR program:\n1. 95 °C 10 min\n2. x 30{\n ... |
109,263 | READDI protocol: Crystallisation of CHIKV nsP3 macrodomain | 1 | dx.doi.org/10.17504/protocols.io.j8nlk8qzdl5r/v2 | https://www.protocols.io/view/readdi-protocol-crystallisation-of-chikv-nsp3-macr-dnxp5fmn | Jasmin Aschenbrenner, Peter Marples, michael fairhead, Andre Schutzer de Godoy, Daren Fearon | TITLE: READDI protocol: Crystallisation of CHIKV nsP3 macrodomain
AUTHORS: Jasmin Aschenbrenner, Peter Marples, michael fairhead, Andre Schutzer de Godoy, Daren Fearon
[DESCRIPTION]
The crystallization protocol and buffer conditions used to obtain reproducible Chikungunya Virus NS3 macrodomain crystals suitable for XC... | ["[Crystallisation experiment] Protein and buffer requirements:\n21.6 µL11 mg/mL \n2.88 mL \n10.08 µL Seeds, dilution 1:100", "[Crystallisation experiment] Dispense 30 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.\nDispense 75 nL11 mg/mL to each lens using the SPT mosquito.\nD... |
null | null | null | dx.doi.org/10.17504/protocols.io.hh5b386 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol was used to check, whether the DNA can be delivered to Eutreptiella gymnastica.</p>
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.nu8dezw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><em>Asterionella formosa</em> is a freshwater diatom and as such as a silica cell wall. Therefore classical protocols for RNA extraction do no work. We have settled this protocol that gives high quality RNA (tested by NGS). This protocol was also applied to marine diatoms, <... | [] |
38,557 | POLAR Express: Pathogen-Oriented Low-cost Assembly & Re-sequencing | 4 | dx.doi.org/10.17504/protocols.io.bhv5j686 | https://www.protocols.io/view/polar-express-pathogen-oriented-low-cost-assembly-bhv5j686 | Brian Glenn St Hilaire | TITLE: POLAR Express: Pathogen-Oriented Low-cost Assembly & Re-sequencing
AUTHORS: Brian Glenn St Hilaire
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [RNA Extraction]
Pellet the beads onto the side of the sample tube using a magnet stand. Incubate for or until the beads have pelleted and the supernata... | ["[RNA Extraction]\nPellet the beads onto the side of the sample tube using a magnet stand. Incubate for or until the beads have pelleted and the supernatant is completely clear. Then, while avoiding the bead pellet, carefully remove the clear supernatant.", "[RNA Extraction]\nAdd MagBead DNA/RNA Wash 1 and mix by us... |
32,302 | Respiratory distress syndrome of the newborn and transient tachypnea of the newborn diagnosis | null | dx.doi.org/10.17504/protocols.io.bbsninde | null | Marconi Augusto Aguiar dos Reis, Roberta Maia C Romanelli, Zilma Reis | TITLE: Respiratory distress syndrome of the newborn and transient tachypnea of the newborn diagnosis
AUTHORS: Marconi Augusto Aguiar dos Reis, Roberta Maia C Romanelli, Zilma Reis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The authors detailed describe the standard procedures for immatur... | ["[Transient tachypnea of the newborn ]\nThe following clinical characteristics characterize the transient tachypnea of the newborn (TTN)", "[Transient tachypnea of the newborn ]\nA. Tachypnea that persists after 2 hours of life, with mild respiratory distress, according to the Silverman Anderson Bulletin, if possible.... |
67,430 | InstaHard: Does Apple Keto Gummies AU Work? | 3 | dx.doi.org/10.17504/protocols.io.261genz6jg47/v1 | https://www.protocols.io/view/instahard-does-apple-keto-gummies-au-work-cd4es8te | Apple Keto Gummies | TITLE: InstaHard: Does Apple Keto Gummies AU Work?
AUTHORS: Apple Keto Gummies
[DESCRIPTION]
Apple Keto Gummies
[STEPS] | [] |
98,457 | OSU TriState SenNet H&E staining of Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections | 1 | dx.doi.org/10.17504/protocols.io.81wgbzpjngpk/v1 | https://www.protocols.io/view/osu-tristate-sennet-h-amp-e-staining-of-formalin-f-dcdz2s76 | Lorena Rosas, Ana L Mora, mauricio.rojas | TITLE: OSU TriState SenNet H&E staining of Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections
AUTHORS: Lorena Rosas, Ana L Mora, mauricio.rojas
[DESCRIPTION]
Hematoxylin and eosin (H&E) stains are essential for recognizing the different tissue types and morphologic changes that contribute to the diagnosis of... | ["[Staining Procedure] Prepare slides", "[Staining Procedure] Place the slides at 60 °C for 30 minutes.", "[Staining Procedure] The solutions are fill in square glass staining jars.", "[Staining Procedure] Place slides in glass staining racks.", "[Staining Procedure] Deparaffinization of tissue slides: Remove remaining... |
86,485 | Strategic Savings in Ligation Sequencing: A Practical Nanopore Library Preparation Workflow | 1 | null | https://www.protocols.io/view/strategic-savings-in-ligation-sequencing-a-practic-cypvxvn6 | Jie Hao Ou, Yin-Tse Huang | TITLE: Strategic Savings in Ligation Sequencing: A Practical Nanopore Library Preparation Workflow
AUTHORS: Jie Hao Ou, Yin-Tse Huang
[DESCRIPTION]
This protocol introduces a cost-effective alternative for end repair and dA-tailing in DNA library preparation, particularly tailored for samples with an N50 of 3 kb. By e... | ["[Homebrew End Repair/dA-Tailing] Add the materials in the following table sequentially.\nMaterialsQuantityDNA800 – 1600 ngH2OBring up to a volume of 43 ul2X PNK/Taq Buffer50 ulATP (25 mM)1 uldNTP (10 mM)5 ulTaq polymerase0.5 ul (2.5 U)T4 PNK0.5 ul (5 U)", "[Homebrew End Repair/dA-Tailing] Incubate at 37 °C for30 min"... |
90,497 | Analysis of nuclei integrity in cultured induced neurons by fluorescence microscopy | 4 | dx.doi.org/10.17504/protocols.io.j8nlko6e1v5r/v1 | https://www.protocols.io/view/analysis-of-nuclei-integrity-in-cultured-induced-n-c4k9yuz6 | Melissa Hoyer, Harper JW | TITLE: Analysis of nuclei integrity in cultured induced neurons by fluorescence microscopy
AUTHORS: Melissa Hoyer, Harper JW
[DESCRIPTION]
The endoplasmic reticulum (ER) has a vast proteomic landscape to preform many diverse functions including protein and
lipid synthesis, calcium ion flux, and inter-organelle communi... | ["[Genetically modify Ngn2-inducible embryonic stem (ES) cell H9 line using Cas9] Genetic editing of Ngn2-inducible ES cells is done using the following protocol\n“Electroporation of Cas9 protein into human pluripotent stem cells” (dx.doi.org/10.17504/protocols.io.br87m9zn)", "[Differentiation of Stable Cell ES H9 line... |
null | null | null | dx.doi.org/10.17504/protocols.io.uvhew36 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
SECTION: Reaction Setup on ice
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SECTION: Reaction Setup on ice
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SECTION: Reaction Setup on ice
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SECTION: Reaction Setup on ice
?.
SECTION: Reaction Setup on ice
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SECTION: Reaction Setup on ice
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SECTION: Reaction Setup on ice
?.
SECTION: Thermocycling conditions
?.
SE... | ["[Reaction Setup on ice] {\"blocks\":[{\"key\":\"1uf1g\",\"text\":\"1. Add 1 \\u00b5L of 70 ng Template DNA. \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[{\"offset\":34,\"length\":1,\"key\":0}],\"data\":[]},{\"key\":\"66ljr\",\"text\":\"\",\"type\":\"unstyled\",\"depth\":0,\"inlineSt... |
97,924 | Immunofluorescence staining protocol with Antigen Retrieval | 1 | dx.doi.org/10.17504/protocols.io.bp2l62nk1gqe/v1 | https://www.protocols.io/view/immunofluorescence-staining-protocol-with-antigen-dbvc2n2w | madalynn.erb Erb | TITLE: Immunofluorescence staining protocol with Antigen Retrieval
AUTHORS: madalynn.erb Erb
[DESCRIPTION]
This protocol details the immunofluorescence staining protocol with antigen retrieval.
[STEPS]
SECTION: Day 1
1. Staining protocol for free floating mouse brain sections.
SECTION: Day 1
2. Wash tissue section... | ["[Day 1] Staining protocol for free floating mouse brain sections.", "[Day 1] Wash tissue sections 3 times (5 minutes each wash) in PBS to remove cryoprotectant solution.", "[Day 1] Mount tissue onto positively charged slides.", "[Day 1] Allow slides to air dry then place in oven set at 37 °C-50 °C 5 min.", "[Day 2]... |
67,682 | Bioluminescence-based Minimum Inhibitory Concentration (MIC) testing of fungal extracts against Escherichia coli | 4 | dx.doi.org/10.17504/protocols.io.6qpvr6jrovmk/v1 | https://www.protocols.io/view/bioluminescence-based-minimum-inhibitory-concentra-cecatase | Shara Van De Pas, Siouxsie Wiles | TITLE: Bioluminescence-based Minimum Inhibitory Concentration (MIC) testing of fungal extracts against Escherichia coli
AUTHORS: Shara Van De Pas, Siouxsie Wiles
[DESCRIPTION]
In this protocol, we describe how to obtain the minimum inhibitory concentration (MIC) of fungal extracts using a bioluminescent derivative of ... | ["[Preparing 96-well plates] We test doubling dilutions of each extract fraction in duplicate with a maximum concentration of 1 mg/mL. Each round of screening also requires a control plate containing the solvent the extract was dissolved in (e.g. DMSO), an antibiotic (to be used as a positive control, e.g. erythromycin... |
36,112 | Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302) | 1 | dx.doi.org/10.17504/protocols.io.bfhqjj5w | https://www.protocols.io/view/determining-genome-targeting-efficiency-using-t7-e-bfhqjj5w | New England Biolabs | TITLE: Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302)
AUTHORS: New England Biolabs
[DESCRIPTION]
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I... | ["[PCR] Set up a 50 µL PCR reaction using ~100 ng as a template. For each amplicon set up 3 PCR reactions using the following templates:\ngDNA from targeted cells (e.g. Cas9, or TALEN transfected cells)\ngDNA from negative control cells (e.g. non-specific DNA transfected cells)\nwater (i.e. no template control)\nPCR us... |
25,536 | Sample Preparation for Illumina MiSeq Dual Index Amplicon Sequencing | null | dx.doi.org/10.17504/protocols.io.468gzhw | null | Máté Vass, Anna Székely | TITLE: Sample Preparation for Illumina MiSeq Dual Index Amplicon Sequencing
AUTHORS: Máté Vass, Anna Székely
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Amplicon sequencing sample preparation for bacteria and microeukaryotes</div></div>
[STEPS]
?. Perform the first PCR (duplicates of each samp... | ["Perform the first PCR (duplicates of each sample) using Illumina adaptor attached primers that target the gene of your choice. Here we present the protocol using the bacterial primers 341F and 805RN, and eukaryotic primers 574*f and 1132r. Bacterial primers (Herlemann et al., 2011):Illumina adapter-N4-341F:5’-ACACTCT... |
48,189 | Staphylococcal Protein-A and Chimeric Protein-LAG sandwich ELISA | 1 | dx.doi.org/10.17504/protocols.io.bta5nig6 | https://www.protocols.io/view/staphylococcal-protein-a-and-chimeric-protein-lag-bta5nig6 | Angel Justiz-Vaillant | TITLE: Staphylococcal Protein-A and Chimeric Protein-LAG sandwich ELISA
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-LAG (PLAG) with different immunoglobulin preparatio... | ["This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-LAG (PLAG) with different immunoglobulin preparations from mammalian and avian species. The 96 well microtiter plate was coated overnight at 4°C with 2 µg/µl per well of SpA in carbonate-bicarbonate buffer pH 9.6.", "The ... |
101,665 | Zika NS5 RdRp His-SUMO construct small scale expression and purification protocol | 1 | dx.doi.org/10.17504/protocols.io.3byl49wx2go5/v1 | https://www.protocols.io/view/zika-ns5-rdrp-his-sumo-construct-small-scale-expre-dfh93j96 | Korvus Wang, michael fairhead, Eleanor Williams | TITLE: Zika NS5 RdRp His-SUMO construct small scale expression and purification protocol
AUTHORS: Korvus Wang, michael fairhead, Eleanor Williams
[DESCRIPTION]
This protocol details the co-expression and purification of Zika NS5 NS5 RNA-dependent RNA polymerase bearing a N-terminal His-SUMO tag at small scale (<6L).
... | ["[Plasmid Transformation] ZVRdRp N-terminal 6His-SUMO tagged co-expression construct was inoculated from its SixPack glycerol stock.", "[Protein expression] When the OD600 reaches approximately 2.0, lower the temperature and shaker speed to and incubate . Harvest in late afternoon on the second day.", "[Protein Pu... |
57,131 | NGS library preparation using NEXTFLEX Rapid Directional RNAseq kit (NOVA-5138-08) for animal tissue samples | 4 | dx.doi.org/10.17504/protocols.io.q26g74843gwz/v1 | https://www.protocols.io/view/ngs-library-preparation-using-nextflex-rapid-direc-b32jqqcn | Yiqiao Li, Magda Bletsa, Ine Boonen, Philippe Lemey | TITLE: NGS library preparation using NEXTFLEX Rapid Directional RNAseq kit (NOVA-5138-08) for animal tissue samples
AUTHORS: Yiqiao Li, Magda Bletsa, Ine Boonen, Philippe Lemey
[DESCRIPTION]
This protocol is used for successful NGS library preparation from total RNA of animal tissue samples.
This method is mainly... | ["[STEP A: RNA Fragmentation] Adjust RNA volume to 14 µL by nuclease-free water.", "[STEP A: RNA Fragmentation] For each reaction combine the following in a nuclease-free microcentrifuge tube or plate:", "[STEP A: RNA Fragmentation] Mix thoroughly by pipetting.", "[STEP A: RNA Fragmentation] Heat for 7-10 minutes at 95... |
null | null | null | dx.doi.org/10.17504/protocols.io.htvb6n6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
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81,460 | 10x Protocols: Visium v1 FFPE Library Construction -- University of Minnesota TMCs (CG000407 Rev D) | 1 | dx.doi.org/10.17504/protocols.io.yxmvm2rpng3p/v1 | https://www.protocols.io/view/10x-protocols-visium-v1-ffpe-library-construction-ctsuwnew | 10x Genomics, Laura J Niedernhofer , David A Bernlohr | TITLE: 10x Protocols: Visium v1 FFPE Library Construction -- University of Minnesota TMCs (CG000407 Rev D)
AUTHORS: 10x Genomics, Laura J Niedernhofer , David A Bernlohr
[DESCRIPTION]
Protocols from 10x Genomics for Visium Spatial Gene Expression v1 chemistry on FPPE samples (without the CytAssist component).
1. Prot... | ["10x protocol CG000407, Revision D (Library construction)", "Additional Protocols/Resources\n \n \nhttps://www.10xgenomics.com/support/spatial-gene-expression-ffpe"] |
50,848 | Next generation shotgun library preparation for Illumina sequencing - low volume | 4 | dx.doi.org/10.17504/protocols.io.bvv8n69w | https://www.protocols.io/view/next-generation-shotgun-library-preparation-for-il-bvv8n69w | cweihe , Julio Avelar-Barragan | TITLE: Next generation shotgun library preparation for Illumina sequencing - low volume
AUTHORS: cweihe , Julio Avelar-Barragan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:center"><span style = "font-weight:bold;">Adapted from: </span><span>https://www.... | ["[PREPARATION]\nTake your DNA extractions from and thaw on ice - spin down on bench spinner.\n-20 °C", "[PREPARATION]\nOptional: Measure DNA concentration of your DNA extractions with Qubit (see Qubit protocol).", "[PREPARATION]\nOptional: Based on the DNA concentrations adjust the samples to ~equal ng for library pr... |
78,870 | Microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons | 4 | dx.doi.org/10.17504/protocols.io.yxmvm2kqog3p/v2 | https://www.protocols.io/view/microscopy-based-evaluation-of-mtkeima-flux-in-hes-cq9wvz7e | Felix Kraus | TITLE: Microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons
AUTHORS: Felix Kraus
[DESCRIPTION]
Protocol for the microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons
[STEPS]
SECTION: Differentiation of iNeurons
1. Day 0: Treat AAVS1-TRE3G-NGN2 cells w... | ["[Differentiation of iNeurons] Day 0: Treat AAVS1-TRE3G-NGN2 cells with Accutase and plate the dissociated cells in matrigel-coated 6-well plates (2x105 cells/well) in ND1 Medium supplemented with Y27632 (10 μM). \nND1 Medium: \nDMEM/F12 \nN2 (100x) 1x \nBDNF 10 ng/ml \nNT3 10 n... |
null | null | null | dx.doi.org/10.17504/protocols.io.rufd6tn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Glutamate is enzymatically measured in the cerebrospinal fluid by monitoring the fluorescence increase due to NADPH<sup>+ </sup>production in the presence of glutamate dehydrogenase and NADP<sup>+ </sup>on a spectrofluorimeter (Shimadzu RF-5301PC, Japan).</p>
[STEPS] | [] |
94,602 | N_terminal protein labeling | 1 | dx.doi.org/10.17504/protocols.io.rm7vzxpbrgx1/v1 | https://www.protocols.io/view/n-terminal-protein-labeling-c8mizu4e | Patricia Yuste Checa, F Ulrich Hartl | TITLE: N_terminal protein labeling
AUTHORS: Patricia Yuste Checa, F Ulrich Hartl
[DESCRIPTION]
This protocol details how to efficiently label a protein at the N-terminus using Clusterin protein as example.
[STEPS]
SECTION: N-terminal protein labeling
1. Exchange the protein buffer to Labeling buffer using a Nap-5 co... | ["[N-terminal protein labeling] Exchange the protein buffer to Labeling buffer using a Nap-5 column (Thermo Fisher Scientific, 45-000-151). Equilibrate the column with 10 column volumes (CV: 1 mL). Load the protein onto the column and elute the protein with the corresponding amount of Labeling buffer following the colu... |
96,824 | Cryo-fixation and resin embedding of biological samples for electron microscopy and chemical imaging | 0 | dx.doi.org/10.17504/protocols.io.bp2l62kndgqe/v1 | https://www.protocols.io/view/cryo-fixation-and-resin-embedding-of-biological-sa-dasy2efw | Benoit GALLET, Christine Moriscot, Guy Schoehn, Johan Decelle | TITLE: Cryo-fixation and resin embedding of biological samples for electron microscopy and chemical imaging
AUTHORS: Benoit GALLET, Christine Moriscot, Guy Schoehn, Johan Decelle
[DESCRIPTION]
Electron microscopy and chemical imaging are now essential in life science to access the interior of a cell and unveil its str... | ["[Sample preparation of cells and tissue] Cells in suspensions (e.g. microalgae, bacteria, mammal cells): Concentration of cells before freezing is an essential step in order to maximize the number of cells in the resin block and so the downstream microscopy observation of multiple cells. The challenge is to concentra... |
55,459 | Topographical mapping of sympathetic postganglionic innervation of mouse heart | 1 | dx.doi.org/10.17504/protocols.io.b2ebqban | https://www.protocols.io/view/topographical-mapping-of-sympathetic-postganglioni-b2ebqban | Ariege Bizanti, Yuanyuan Zhang, Kohlton Bendowski, Jin Chen, Mahyar Osanlouy, Maci Heal, Zixi Jack Cheng | TITLE: Topographical mapping of sympathetic postganglionic innervation of mouse heart
AUTHORS: Ariege Bizanti, Yuanyuan Zhang, Kohlton Bendowski, Jin Chen, Mahyar Osanlouy, Maci Heal, Zixi Jack Cheng
[DESCRIPTION]
This protocol describes the process of mapping the topographical organization of tyrosine hydroxy... | ["Animals \nMale C57BL/6J mice (Jackson laboratory), n=6 were used. Animals were kept in the animal room with dark/light cycle set to 12/12 hours and water and food were supplied ad libitum. All procedures were carried out under the ethical guidelines of University of Central Florida and approved by the Animal Care an... |
97,158 | Fiber Photometry during Sleep | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8xw3lmk/v1 | https://www.protocols.io/view/fiber-photometry-during-sleep-da5e2g3e | daniel.dautan daniel, Per Svenningsson | TITLE: Fiber Photometry during Sleep
AUTHORS: daniel.dautan daniel, Per Svenningsson
[DESCRIPTION]
Measurement of photometry during sleep using AAVs
[STEPS]
SECTION: Fiber Photometry- Virus Injection
1. Preparation:
SECTION: Fiber Photometry- Virus Injection
4. Injection Site Determination:
SECTION: Fiber Photometry... | ["[Fiber Photometry- Virus Injection] Preparation:", "[Fiber Photometry- Virus Injection] Injection Site Determination:", "[Fiber Photometry- Virus Injection] Surgical Site Preparation:", "[Fiber Photometry- Virus Injection] Anesthesia and Animal Preparation:", "[Fiber Photometry- Virus Injection] Syringe Preparation:"... |
null | null | null | dx.doi.org/10.17504/protocols.io.c3pymm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in <a href="https://www.protocols.io/view/DNase-I-Treatment-c3myk5" target="_blank">DNase I Treatment</a> protocol for DNase inactivation
[GUIDELINES]
Note: EGTA tetratsodium salt is far easier to get into solution than the disodium salt. It is possible to get to 1.5M w... | [] |
109,250 | ICD-11 post-traumatic stress disorder (PTSD) and complex post-traumatic stress disorder (CPTSD) assessed with the International Trauma Interview (ITI) and International Trauma Questionnaire (ITQ) for trauma-affected civilians and refugees in two outpatient | 0 | dx.doi.org/10.17504/protocols.io.rm7vzjz98lx1/v1 | https://www.protocols.io/view/icd-11-post-traumatic-stress-disorder-ptsd-and-com-dnxa5fie | Maja Bruhn, Hinuga Sandahl, Mads Christian Jensen, Henriette Laugesen, Sofie Folke, Jessica Carlsson | TITLE: ICD-11 post-traumatic stress disorder (PTSD) and complex post-traumatic stress disorder (CPTSD) assessed with the International Trauma Interview (ITI) and International Trauma Questionnaire (ITQ) for trauma-affected civilians and refugees in two outpatient
AUTHORS: Maja Bruhn, Hinuga Sandahl, Mads Christian Jens... | [] |
57,513 | Fluorescence-activated nuclei sorting (FANS) of purified neural cell populations from mouse cortex for multi-omic profiling | 4 | dx.doi.org/10.17504/protocols.io.dm6gpbwndlzp/v1 | https://www.protocols.io/view/fluorescence-activated-nuclei-sorting-fans-of-puri-b4ehqtb6 | Stefania S Policicchio, Isabel Castanho, Barry Chioza, Jonathan P Davies, Joe Burrage, Jonathan Mill, Emma L Dempster | TITLE: Fluorescence-activated nuclei sorting (FANS) of purified neural cell populations from mouse cortex for multi-omic profiling
AUTHORS: Stefania S Policicchio, Isabel Castanho, Barry Chioza, Jonathan P Davies, Joe Burrage, Jonathan Mill, Emma L Dempster
[DESCRIPTION]
Increased understanding of the functio... | ["[Nuclear prep for FACS separation (using NeuN, PU.1 and Hoechst)] In our hands the protocol below yields ~60,000 NeuN +ve (neuron enriched), ~5,000 PU.1 +ve (microglial enriched) and ~20,000 double negative (NeuN-ve/PU.1-ve; oligodendrocyte enriched) nuclei per ≤ 100 mg of frozen mouse cortex tissue. Recovery might v... |
21,771 | Microorganisms and culture conditions | null | dx.doi.org/10.17504/protocols.io.zhjf34n | null | Lis Rocha | TITLE: Microorganisms and culture conditions
AUTHORS: Lis Rocha
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Many efforts have been made to understand the pathogenesis of bovine mastitis to reduce losses and promote animal welfare. </span><span style = "font-style:italic;">Staphylococcus au... | ["[Growing and storing conditions]\nThe bacteria used in this study were maintained on BHI agar (Brain Heart Infusion, BHI HiMedia, Mumbai, India) at 37 °C", "[Growing and storing conditions]\nstored in the long term in BHI containing 20% glycerol."] |
98,518 | BIDMC-TMC Nuclei Isolation from Frozen Cervix for Single Cell RNA-Seq | 0 | dx.doi.org/10.17504/protocols.io.36wgqn4rxgk5/v1 | https://www.protocols.io/view/bidmc-tmc-nuclei-isolation-from-frozen-cervix-for-dcfw2tpe | Luciano G Martelotto, Antonella Arruda de Amaral, Nikolaos Kalavros, Ioannis Vlachos, Shuoshuo Wang | TITLE: BIDMC-TMC Nuclei Isolation from Frozen Cervix for Single Cell RNA-Seq
AUTHORS: Luciano G Martelotto, Antonella Arruda de Amaral, Nikolaos Kalavros, Ioannis Vlachos, Shuoshuo Wang
[DESCRIPTION]
As a fibromuscular organ, human cervix samples are naturally resilient to withstand strains and deformation during preg... | ["[Sample Preparation] Ensure the samples are free of OCT if they were previously embedded. Coarsely trim off the peripheral OCT and rinse the sample core with RNase-free chilled PBS thoroughly before processing.", "[Sample Preparation] Pre-cool a mortar and pestle by filling with sufficient liquid nitrogen;", "[Sample... |
67,569 | Centriflaken: an automated data analysis pipeline for assembly and in silico analyses of food-borne pathogens from metagenomic samples | 5 | dx.doi.org/10.17504/protocols.io.kxygxzdbwv8j/v3 | https://www.protocols.io/view/centriflaken-an-automated-data-analysis-pipeline-f-cd8rs9v6 | Kranti Konganti, Vishal Thovarai, Meghan Maguire, Julie A. Kase, Narjol Gonzalez-Escalona | TITLE: Centriflaken: an automated data analysis pipeline for assembly and in silico analyses of food-borne pathogens from metagenomic samples
AUTHORS: Kranti Konganti, Vishal Thovarai, Meghan Maguire, Julie A. Kase, Narjol Gonzalez-Escalona
[DESCRIPTION]
Rapid and comprehensive analysis of metagenomic data from any sa... | ["[Step 1] Create an account and Login:\n\nIf you do not already have an account on GalaxyTrakr, please create one by visiting this URL: https://account.galaxytrakr.org/Account/Register", "[Step 1] Once your account is activated, login by visiting https://galaxytrakr.org.", "[Step 2] Create a new history:\n \n\n \n\n\n... |
41,727 | Scoping review protocol on the use of telephone in the implementation of citizen participation in the processes of developing health system strengthening reforms/policies in Sub-Saharan Africa (participation in decision-making) | 1 | dx.doi.org/10.17504/protocols.io.bky7kxzn | https://www.protocols.io/view/scoping-review-protocol-on-the-use-of-telephone-in-bky7kxzn | Wendkouni Adelphe Sabine OUEDRAOGO, Sandrine Biau-Lalanne, Emmanuel Bonnet, Valery Ridde | TITLE: Scoping review protocol on the use of telephone in the implementation of citizen participation in the processes of developing health system strengthening reforms/policies in Sub-Saharan Africa (participation in decision-making)
AUTHORS: Wendkouni Adelphe Sabine OUEDRAOGO, Sandrine Biau-Lalanne, Emmanuel Bonnet, ... | ["[JUSTIFICATION]\nRationale\n\t\t\t\t\t\t\t .justify:after {\n\t\t\t\t\t\t\t content: \"\";\n\t\t\t\t\t\t\t display:inline-block;\n\t\t\t\t\t\t\t width: 100%;\n\t\t\t\t\t\t\t }\n\t\t\t\t\t\t\tAccountability and empowerment of populations are two notions that are increasingly important in sustainable develop... |
null | null | null | dx.doi.org/10.17504/protocols.io.crgv3v | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This electroporation protocol is for use with the NEB Turbo Electrocompetent E. coli cells (<a href="https://www.neb.com/products/c2986-neb-turbo-electrocompetent-e-coli" target="_blank">C2986</a>). These cells are suitable for high efficiency electroporation and rapid... | [] |
89,890 | 812.1 Lung FFPE OMAP Multiplexed Immunofluorescence Phenocycler-Fusion® Antibody Validation Protocol | 4 | dx.doi.org/10.17504/protocols.io.36wgq3b9olk5/v1 | https://www.protocols.io/view/812-1-lung-ffpe-omap-multiplexed-immunofluorescenc-c32ayqae | Jeffrey Purkerson, Gloria S Pryhuber, Heidie Huyck, gail.deutsch | TITLE: 812.1 Lung FFPE OMAP Multiplexed Immunofluorescence Phenocycler-Fusion® Antibody Validation Protocol
AUTHORS: Jeffrey Purkerson, Gloria S Pryhuber, Heidie Huyck, gail.deutsch
[DESCRIPTION]
This protocol describes validation of a 34-antibody panel used for multiplexed immunofluorescent (MxF) staining and imaging... | ["[Tissue Sections] Lung serial sections, and a tonsil section were prepared in FFPE as described in dx.doi.org/10.17504/protocols.io.kxygxejwdv8j/v2", "[Labeling of Tissue Sections with barcode conjugated Ab or unconjugated Ab] One of two serial healthy Lung sections (D016-RLL-11B2-6), a known diseased Lung section (D... |
62,002 | Protocol for use of Vertical Modified Moore Swab (VMMS) to Isolate Salmonella from Surface Water | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy7q5lx1/v1 | https://www.protocols.io/view/protocol-for-use-of-vertical-modified-moore-swab-v-b8ssrwee | Autumn L Kraft, Manan Sharma, Jonathan G Frye, James E Wells, Abasiofiok Mark Ibekwe, NARMS EWG | TITLE: Protocol for use of Vertical Modified Moore Swab (VMMS) to Isolate Salmonella from Surface Water
AUTHORS: Autumn L Kraft, Manan Sharma, Jonathan G Frye, James E Wells, Abasiofiok Mark Ibekwe, NARMS EWG
[DESCRIPTION]
This protocol describes the use of vertical modified Moore swab (VMMS) to recover and isol... | ["[VMMS Cartridge Assembly: End piece Assembly] Apply Purple Primer (Oatey 30756) to ½ to ¾ in length of the bottom part of the cylindrical section of part b (bushing, 1 ½” x ½” PVC S40 SPxFIP Bush).", "[VMMS Cartridge Assembly: End piece Assembly] Apply purple primer to the inside, non-threaded end of part c (male ada... |
87,500 | JMN-MSMP Muscle RNA Isolation | 4 | null | https://www.protocols.io/view/jmn-msmp-muscle-rna-isolation-czpkx5kw | ccherry | TITLE: JMN-MSMP Muscle RNA Isolation
AUTHORS: ccherry
[DESCRIPTION]
Isolation of RNA from muscle samples
[STEPS]
SECTION: Isolation of RNA
1. Muscle sections were submerged in Trizol Reagent (Thermo Fisher Scientific), minced, and grinded.
SECTION: Isolation of RNA
2. Subsequent RNA isolation was performed using the ... | ["[Isolation of RNA] Muscle sections were submerged in Trizol Reagent (Thermo Fisher Scientific), minced, and grinded.", "[Isolation of RNA] Subsequent RNA isolation was performed using the RNAeasy Mini Kit (Qiagen) following manufacturers protocol."] |
57,742 | Thawing of hPSCs grown on MEFs | 4 | dx.doi.org/10.17504/protocols.io.b4mnqu5e | https://www.protocols.io/view/thawing-of-hpscs-grown-on-mefs-b4mnqu5e | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Thawing of hPSCs grown on MEFs
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the standard procedure of thawing human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).
General notes
1. Throughout this proto... | ["Prepare one 6-well MEFs plate for each vial of frozen hPSCs", "Place vial of frozen hPSCs in 37 °C water bath with constant agitation.", "Pipette thawed cell suspension into 10 ml pre-warmed hPSCs medium.", "Centrifuge 200-300 x g, 5 min", "While cells are spinning, aspirate the MEFs medium from the MEFs plates and... |
48,814 | Turf Umbrella Runoff Study: Total Suspended Solids (TSS) | 1 | null | https://www.protocols.io/view/turf-umbrella-runoff-study-total-suspended-solids-btwnnpde | USDA | TITLE: Turf Umbrella Runoff Study: Total Suspended Solids (TSS)
AUTHORS: USDA
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Turf Umbrella Runoff Study: Total Suspended Solids (TSS)</div></div>
[STEPS]
?. Remove samples from the refrigerator. Bring to room temperature.
?. Assemble filtering appara... | ["Remove samples from the refrigerator. Bring to room temperature.", "Assemble filtering apparatus to vacuum.", "Record the aluminum tin ID # on data sheet.", "Using a tweezers, place an oven-baked filter (either side facing up) into the aluminum tin. Discard the blue paper between filters.If you need more baked filter... |
60,247 | Inorganic polyphosphate from microalgae: A DAPI-based estimation in microtiter plate | 1 | null | https://www.protocols.io/view/inorganic-polyphosphate-from-microalgae-a-dapi-bas-b63xrgpn | Yingyu Hu, Zoe V Finkel | TITLE: Inorganic polyphosphate from microalgae: A DAPI-based estimation in microtiter plate
AUTHORS: Yingyu Hu, Zoe V Finkel
[DESCRIPTION]
The DAPI-based fluorometric estimation of polyphosphate from microalgae has been widely used in field samples since the method was published by Martin P. et al., where fluoresce... | ["[Sample collection] Filter microalgae in liquid media onto precombusted GFF filters, using gentle vacuum pressure (5 inches Hg).", "[Sample collection] Rinse sample with filtered seawater", "[Sample collection] Place sample filters in cryogenic vials", "[Sample collection] Filter blank media (without cells) through p... |
24,429 | Protocol for Albuwell M kit: Murine Microalbuminuria ELISA By Exocell Inc | null | dx.doi.org/10.17504/protocols.io.34mgqu6 | null | Kathi Burke, Peter Reifsnyder | TITLE: Protocol for Albuwell M kit: Murine Microalbuminuria ELISA By Exocell Inc
AUTHORS: Kathi Burke, Peter Reifsnyder
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Albuwell M is an indirect competitive ELISA design... | ["[Urine Collection Protocols]\nSpot Urine Between 8:00 and 10:00 a.m., the mouse is picked up and induced to urinate into a 1.5 ml microfuge tube. If this is not successful, or not enough is collected, attempts on successive days can be combined (urine should be stored at 4ºC between attempts). Another way to collect ... |
60,160 | Run Clearmap 2 docker | 5 | dx.doi.org/10.17504/protocols.io.yxmvmn9pbg3p/v1 | https://www.protocols.io/view/run-clearmap-2-docker-b6y8rfzw | Moritz Negwer | TITLE: Run Clearmap 2 docker
AUTHORS: Moritz Negwer
[DESCRIPTION]
This protocol is a supplement to our upcoming publication "FriendlyClearMap: An optimized toolkit for mouse brain mapping and analysis".
In this protocol, we describe in detail how to run Clearmap2's CellMap portion in a Docker container.
[STEPS]
SE... | ["[Docker Setup] If you haven't already, download and set up Docker Desktop for Windows. This requires administrator privileges and will also install the Windows Subsystem for Linux (WSL2). \n\nDownload: \nhttps://www.docker.com/products/docker-desktop \n\nThen, download the docker container from our repository: \nXXX ... |
7,901 | DNA Extraction from Filters using QIAgen DNeasy and QIAshredder | null | dx.doi.org/10.17504/protocols.io.jx5cpq6 | null | Melissa Duhaime, Rachel Cable | TITLE: DNA Extraction from Filters using QIAgen DNeasy and QIAshredder
AUTHORS: Melissa Duhaime, Rachel Cable
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">From the Qiagen DNeasy Handbook (07/2006)</div><div class = "text-block"><a href="http://www.qiagen.com/products/catalog/sample-technologies/d... | ["[Reagent Preparation]\nDo the following once per kit:Add 25 mL ethanol to Buffer AW1; check box on bottle (✔).Add 30 mL ethanol to Buffer AW2; check box on bottle (✔). Do the following in preparation for each extraction:Confirm Buffers ATL and AL are completely in solution; warm to 56° C if necessary.Prepare a soluti... |
67,443 | https://www.facebook.com/primaweightlossdragonsdenUnitedKingdom/ | 1 | dx.doi.org/10.17504/protocols.io.4r3l2ow84v1y/v1 | https://www.protocols.io/view/https-www-facebook-com-primaweightlossdragonsdenun-cd4ts8wn | alexballosz | TITLE: https://www.facebook.com/primaweightlossdragonsdenUnitedKingdom/
AUTHORS: alexballosz
[DESCRIPTION]
Prima Weight Loss Dragons Den UK
Prima Weight Loss Dragons Den UK is a company that helps people who are interested in using their fitness knowledge to make more money. They offer an online blog and video cours... | ["[https://www.facebook.com/primaweightlossdragonsdenUnitedKingdom/]"] |
71,188 | Microscopy-based pUb-coverage measurements of mitochondria in iNeurons | 4 | dx.doi.org/10.17504/protocols.io.5qpvory2bv4o/v1 | https://www.protocols.io/view/microscopy-based-pub-coverage-measurements-of-mito-chrut56w | Felix Kraus | TITLE: Microscopy-based pUb-coverage measurements of mitochondria in iNeurons
AUTHORS: Felix Kraus
[DESCRIPTION]
Microscopy-based pUb-coverage measurements of mitochondria in iNeurons
[STEPS]
SECTION: Differentiation of iNeurons
1. Day 0: Treat AAVS1-TRE3G-NGN2 cells with Accutase and plate the dissociated cells in m... | ["[Differentiation of iNeurons] Day 0: Treat AAVS1-TRE3G-NGN2 cells with Accutase and plate the dissociated cells in matrigel-coated 6-well plates (2x105 cells/well) in ND1 Medium supplemented with Y27632 (10 μM). \nND1 Medium: \nDMEM/F12 \nN2 (100x) 1x \nBDNF 10 ng/ml \nNT3 10 n... |
33,707 | Single-gene short-term CRISPR ko viability assay | 1 | null | https://www.protocols.io/view/single-gene-short-term-crispr-ko-viability-assay-bc6jizcn | Benjamin Gaeta, Tsukasa Shibue, Brenton Paolella, Francisca Vazquez | TITLE: Single-gene short-term CRISPR ko viability assay
AUTHORS: Benjamin Gaeta, Tsukasa Shibue, Brenton Paolella, Francisca Vazquez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">The Cancer Dependency Map Project </span><span>is using genome-scale CRISPR loss-of-f... | ["[Assay Development]\n(Day 0) Template/Planning: create a copy of the \"Viability Assay Optimization\" template and fill out the information.", "[Assay Development]\n(Day 0) Make Viral Master Plate: prepare (a) master plate(s) of BRD003 lenti-virus that contains the following volume of virus per 40 uL of total volume ... |
102,396 | Maintenance & Differentiation: Embryonic Mouse Hippocampal Cells (CLU198) | 0 | dx.doi.org/10.17504/protocols.io.bp2l628wrgqe/v1 | https://www.protocols.io/view/maintenance-amp-differentiation-embryonic-mouse-hi-df843ryw | Md Razaul Karim | TITLE: Maintenance & Differentiation: Embryonic Mouse Hippocampal Cells (CLU198)
AUTHORS: Md Razaul Karim
[DESCRIPTION]
This protocol details the maintenance & differentiation of Embryonic Mouse Hippocampal Cells (CLU198).
[STEPS]
SECTION: Maintenance
1. For regular maintenance of CLU cells, use DMEM full media.... | ["[Maintenance] For regular maintenance of CLU cells, use DMEM full media.", "[Differentiation (CLU 198): Takes about a week of differentiation.] Day-01 (Mon): Plating with DMEM full media.\n\nWarm DMEM full media, PBS, and Trypsin in the 37 °C bead bath for 30 min. Clean the working area by using 70% ethanol.", "[Diff... |
null | null | null | dx.doi.org/10.17504/protocols.io.k7vczn6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.sh5eb86 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Before starting prepare the following</strong>:</p>
<ul>
<li>Measure the Luciferase plasmid concentration using NanoDrop and Qubit.</li>
<li>Design the PCR primers for the gene of interest with the RE (restriction enzyme) sequence at the 5’.</li>
<li>Add 3 to 4 nucleo... | [] |
87,216 | Nuclear Isolation and Purification Protocol for Single-Cell Methods | 4 | dx.doi.org/10.17504/protocols.io.5qpvo3kpxv4o/v1 | https://www.protocols.io/view/nuclear-isolation-and-purification-protocol-for-si-czeqx3dw | Guillermo Barreto Corona, Amelia Hall | TITLE: Nuclear Isolation and Purification Protocol for Single-Cell Methods
AUTHORS: Guillermo Barreto Corona, Amelia Hall
[DESCRIPTION]
This protocol describes how to isolate and purify nuclei from solid tissue for use in single-cell library construction methods. The resulting nuclei are suitable for a variety of sing... | ["[1. Buffer Preparation] The buffers that are noted with a '*' such as HDT-2RI, HB, and NSB are calculated for 2.5 reactions and should be adjusted for more reactions accordingly. The buffers that are able to be stored can be considered as stock buffers and are good for several iterations of the protocol. Make more PB... |
106,962 | AAV titration with qPCR | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1n47lmk/v1 | https://www.protocols.io/view/aav-titration-with-qpcr-dkps4vne | Gerard Michael Coughlin | TITLE: AAV titration with qPCR
AUTHORS: Gerard Michael Coughlin
[DESCRIPTION]
Titration of AAV genomes in a purified sample is critical for ensuring accurate dosing. Titering AAV samples is typically accomplished by first treating samples with a DNase to degrade DNA not contained within the AAV capsid (i.e. unencapsid... | ["[Sample preparation] With a P10 pipette, place 2 µL of AAV sample into wells of a 96-well plate in triplicate. Load 2 µL of DPBS + 0.001% Pluronic F-68 into 3 more wells. Do not load samples into wells at the edge of the plate. Add 50 µL of DNase I solution (Step 5) to wells with AAV or DPBS + 0.001% Pluronic F-68. S... |
70,208 | FSQC Protocol | 1 | dx.doi.org/10.17504/protocols.io.kxygx9m6wg8j/v1 | https://www.protocols.io/view/fsqc-protocol-cgs8twhw | Saashi Bedford | TITLE: FSQC Protocol
AUTHORS: Saashi Bedford
[DESCRIPTION]
This protocol is a guide to conducting a visual quality control (QC) of Freesurfer outputs. Quality control is important to make sure that the cortical surface reconstruction and grey and white matter classification are accurate.
To examine outputs, ten image... | ["[Instructions] Download, save and unzip the Image Rating QC app from https://github.com/sbedford0/imageratingQCApp", "[Instructions] Follow the instructions in the “readme” file to run the program:\nThe first time you run the program, in a terminal, run ‘npm install’ in the app directory\nRun ‘npm run build’ to build... |
17,448 | Transient CRISPR-Cas9 Coupled with Electroporation Protocol | 1 | dx.doi.org/10.17504/protocols.io.bp2l64x2kvqe/v1 | https://www.protocols.click/view/transient-crispr-cas9-coupled-with-electroporation-vage2bw | Amy Gladfelter | TITLE: Transient CRISPR-Cas9 Coupled with Electroporation Protocol
AUTHORS: Amy Gladfelter
[DESCRIPTION]
Transient CRISPR-Cas9 transformation of Cryptococcus neoformans.
[GUIDELINES]
References
1. Lin, X., et al., Generation of stable mutants and targeted gene deletion strains in Cryptococcus neoformans through elec... | ["PCR amplification of CAS9, sgRNA, your construct. \n \n \nCAS9: Use plasmid pXL1-CAS9-HYG as template with primers CAS9-F and CAS9-R. (6985 bp)\n\nsgRNA: U6P and sgRNA scaffold \n \nU6pPromoteris PCR amplified using serotype Dgenomic DNA astemplate with primers U6P-F and GOI-sgRNA-R. ~295 bp \nSgRNA scaffold is PCR a... |
63,901 | https://www.jpost.com/promocontent/simpli-acv-keto-gummies-acv-plus-keto-simply-keto-gummies-is-it-legitimate-or-scam-708281 | 5 | dx.doi.org/10.17504/protocols.io.j8nlkk2z6l5r/v1 | https://www.protocols.io/view/https-www-jpost-com-promocontent-simpli-acv-keto-g-cam5sc86 | Donnyqout | TITLE: https://www.jpost.com/promocontent/simpli-acv-keto-gummies-acv-plus-keto-simply-keto-gummies-is-it-legitimate-or-scam-708281
AUTHORS: Donnyqout
[DESCRIPTION]
More Option:-
Shop Now:- https://www.jpost.com/promocontent/simpli-acv-keto-gummies-acv-plus-keto-simply-keto-gummies-is-it-legitimate-or-scam-708281
S... | ["Simpli ACV Keto GummiesEverybody wishes to stay thin, and it is vital for know how one can deal with their ideal body weight or decrease overabundance muscle versus fat. Heaps of individuals across the globe are confronting corpulence or managing loads of fat gathering in the body.\n\nMany individuals consider an exo... |
80,257 | In-solution Digestion of ECM-Enriched Proteins Samples for Mass Spectrometry Analysis | 4 | dx.doi.org/10.17504/protocols.io.81wgbyer1vpk/v1 | https://www.protocols.io/view/in-solution-digestion-of-ecm-enriched-proteins-sam-csk9wcz6 | jconsi, Ikram Isa, Alexandra Naba | TITLE: In-solution Digestion of ECM-Enriched Proteins Samples for Mass Spectrometry Analysis
AUTHORS: jconsi, Ikram Isa, Alexandra Naba
[DESCRIPTION]
The pellet obtained after the decellularization procedure and removal of SDS is highly enriched in insoluble ECM proteins. For further analysis by mass spectrometry, th... | ["[Protein resuspension and reduction] Resuspend the ECM-enriched sample by adding the appropriate volume of 8 M urea (urea is dissolved in 100 mM ammonium bicarbonate) to the ECM-enriched pellet and add Dithiothreitol(DTT) at a final concentration of 10 mM (see Table 1). Incubate with continuous agitation at for 120... |
71,425 | abcde | 1 | dx.doi.org/10.17504/protocols.io.j8nlkwkb5l5r/v1 | https://www.protocols.io/view/abcde-chy9t7z6 | Nicolas Nitsche | TITLE: abcde
AUTHORS: Nicolas Nitsche
[DESCRIPTION]
new test protocol
[STEPS]
SECTION: Day 1
1. add 5 ul buffer
SECTION: Day 1
2. add 5 µL and centrifuge, add
SECTION: Day 1
3. pipette buffer
SECTION: Day 1
4. step 4
SECTION: Day 1
5. add 5 µL and centrifuge
SECTION: Day 1
6. pipette buffer
SECTI... | ["[Day 1] add 5 ul buffer", "[Day 1] add 5 µL and centrifuge, add", "[Day 1] pipette buffer", "[Day 1] step 4", "[Day 1] add 5 µL and centrifuge", "[Day 1] pipette buffer", "[Day 1] step 4", "[Day 1]", "[Day 1]", "[Day 1]", "[Day 1]", "[Day 1]", "[Day 1]", "[Day 1]", "[Day 2] add", "[day 3]"] |
90,506 | Testing ER stress induction in Cultured Induced Neurons via measuring ATF4 protein level or XBP-1 mRNA splicing | 4 | dx.doi.org/10.17504/protocols.io.261ged94ov47/v1 | https://www.protocols.io/view/testing-er-stress-induction-in-cultured-induced-ne-c4miyu4e | Melissa Hoyer, Harper JW | TITLE: Testing ER stress induction in Cultured Induced Neurons via measuring ATF4 protein level or XBP-1 mRNA splicing
AUTHORS: Melissa Hoyer, Harper JW
[DESCRIPTION]
The endoplasmic reticulum (ER) has a vast proteomic landscape to preform many diverse functions including protein and
lipid synthesis, calcium ion flux,... | ["[Genetically modify Ngn2-inducible embryonic stem (ES) cell H9 line using Cas9] Genetic editing of Ngn2-inducible ES cells is done using the following protocol\n“Electroporation of Cas9 protein into human pluripotent stem cells” (dx.doi.org/10.17504/protocols.io.br87m9zn)", "[Differentiate Stable Cell ES H9 line to i... |
94,029 | Immunocytochemistry for the characterization of hiPSC to Motor Neuron differentiation | 4 | dx.doi.org/10.17504/protocols.io.6qpvr3zbzvmk/v2 | https://www.protocols.io/view/immunocytochemistry-for-the-characterization-of-hi-c73mzqk6 | Mallory Wright, ckremitz, William J Buchser | TITLE: Immunocytochemistry for the characterization of hiPSC to Motor Neuron differentiation
AUTHORS: Mallory Wright, ckremitz, William J Buchser
[DESCRIPTION]
This immunocytochemistry protocol is used for the characterization of IPSC differentiation into motor neurons using several biomarkers: neuroepithelial cells (... | ["Remove the medium from your cells", "FIX - Dilute 32% Paraformaldehyde solution to 4% PFA in 1X phosphate-buffered saline (PBS)", "Add 100uL of 4% PFA to each well in the 96-well plate. (1ml if using a 6-well plate). Incubate for 15 min at room temperature.", "Remove the fixative solution and wash with 1XPBS at 100ul... |
13,231 | Bioinformatics: A rational combine approach used for the identification and in-vitro activity evaluation of potent β-Glucuronidase inhibitors | null | dx.doi.org/10.17504/protocols.io.q6pdzdn | null | Dr.Maria Yousuf | TITLE: Bioinformatics: A rational combine approach used for the identification and in-vitro activity evaluation of potent β-Glucuronidase inhibitors
AUTHORS: Dr.Maria Yousuf
[STEPS] | [] |
96,673 | Rapid Sequencing gDNA Barcoding RBK2004 | 1 | dx.doi.org/10.17504/protocols.io.6qpvr46zogmk/v2 | https://www.protocols.io/view/rapid-sequencing-gdna-barcoding-rbk2004-dam92c96 | Carlos Carlos Goller, Carly Sjogren | TITLE: Rapid Sequencing gDNA Barcoding RBK2004
AUTHORS: Carlos Carlos Goller, Carly Sjogren
[DESCRIPTION]
This protocol has been adapted from Oxford Nanopore Technologies.
You have isolated, purified, and quantified DNA from your bacterial isolate. You have also worked with your bacterium and grown in the presence o... | ["[Library Preparation] Thaw kit components at Room temperature , spin down briefly using a microfuge, and mix by pipetting as indicated by the table below: \n\nFragmentation Mix RB01-12: not frozen, briefly spin down, mix well by pipetting \nRapid Adapter (RAP): not frozen, briefly spin down, mix well by pipetting \nS... |
null | null | null | dx.doi.org/10.17504/protocols.io.nmgdc3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol was used for the molecular profiling of the soil fungal community of two winter wheat fields as a part of a long-term field trial in Germany with following Illumina Amplicon Sequencing. Besides different pre-crops, the effects of soil management and fertilizatio... | [] |
56,717 | In vitro germination of Austropuccinia psidii urediniospores | 4 | dx.doi.org/10.17504/protocols.io.3byl4b73jvo5/v1 | https://www.protocols.io/view/in-vitro-germination-of-austropuccinia-psidii-ure-b3mmqk46 | Alyssa M Martino, Rebecca M Degnan | TITLE: In vitro germination of Austropuccinia psidii urediniospores
AUTHORS: Alyssa M Martino, Rebecca M Degnan
[DESCRIPTION]
Optimisation of Austropuccinia psidii urediniospore germination for use in RNA extraction and cytogenetics.
[STEPS]
SECTION: Spore collection, desiccation, and storage
2. Move fresh spores... | ["[Spore collection, desiccation, and storage] Move fresh spores to a glass petri dish with no lid. Transfer petri dish to a desiccator with silica gel beads for 24 - 48 hours to dry the spores.", "[Inocula preparation and plating] In a 15 mL centrifuge tube, make up inocula to a concentration of approximately 1 mg/mL ... |
51,097 | In vitro transcription of guide RNAs and 5'-triphosphate removal | 1 | dx.doi.org/10.17504/protocols.io.n2bvjyp5vk5w/v15 | https://www.protocols.io/view/in-vitro-transcription-of-guide-rnas-and-5-39-trip-bv5zn876 | Moritz F Schlapansky, Eric Aird, Mark Dewitt, Julia Wong, Beeke Wienert | TITLE: In vitro transcription of guide RNAs and 5'-triphosphate removal
AUTHORS: Moritz F Schlapansky, Eric Aird, Mark Dewitt, Julia Wong, Beeke Wienert
[DESCRIPTION]
sgRNA template assembly, in vitro T7 transcription, and sgRNA column cleanup to remove 5'-triphosphate groups
[GUIDELINES]
The primers used are: o... | ["Move spin column to an RNAse-free 1.5 ml microfuge tube\nAdd 33 µl DEPC-treated H2O; spin 1 min\nOptional: Repeat the elution to collect any remaining RNA on the column and increase RNA concentration.", "Move spin column to a new collection tube and spin for 1 min at 10.000 g to dry the membrane completely.", "Add 50... |
48,284 | Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030) | 1 | dx.doi.org/10.17504/protocols.io.btd4ni8w | https://www.protocols.io/view/oligonucleotide-cleanup-using-monarch-pcr-amp-dna-btd4ni8w | New England Biolabs | TITLE: Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The </span><a href="https://www.neb.com/protocols/2015/11/23/monarch-pcr-and-dna-cleanup-kit-protocol" style = "text-dec... | ["[Oligonucleotide Cleanup]\nAll centrifugation steps should be carried out at 16,000 xg. (~13K RPM in a typical microcentrifuge). This ensures all traces of buffer are eluted at each step.", "[Buffer Preparation]\nAdd ethanol to Monarch DNA Wash Buffer prior to use (4 volumes of ≥ 95% ethanol per volume of Monarch DNA... |
62,139 | Making Blood Agar Plates | 1 | dx.doi.org/10.17504/protocols.io.n2bvj6p7xlk5/v1 | https://www.protocols.io/view/making-blood-agar-plates-b8w3rxgn | Laura Sycuro, Ramon Cortez, sakonsch | TITLE: Making Blood Agar Plates
AUTHORS: Laura Sycuro, Ramon Cortez, sakonsch
[DESCRIPTION]
Purpose
Blood agar is agar base enriched with 5% blood. It is an enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties.
Key concepts
The agar base can v... | ["[Pouring] Pick up media from downstairs shortly after run completes (they should place it in a 55 °C incubator downstairs after the run). \n \n \nPlace in 55 °C water bath for 20-30 minutes.\nMedia is ready to pour when bottle is warm, but not hot to the touch.", "[Pouring] Mix blood (at room temperature) by gently ... |
66,349 | Bridport Health Reviews - Powerfully Detoxifies The Liver, Lose Liver Fat And Improve Gut Health! | 3 | dx.doi.org/10.17504/protocols.io.j8nlkkjk6l5r/v1 | https://www.protocols.io/view/bridport-health-reviews-powerfully-detoxifies-the-cc2msyc6 | cheddithepeon | TITLE: Bridport Health Reviews - Powerfully Detoxifies The Liver, Lose Liver Fat And Improve Gut Health!
AUTHORS: cheddithepeon
[DESCRIPTION]
Product Name - Bridport Health
Ingredients - Milk Thistle, Beetroot, Artichoke Extract & More.
Category - Liver Support Supplement
Main Benefits - Helps Protect The Liver Fro... | [] |
83,159 | Open Field test to assess spontaneous locomotion behavior in parkinsonian mice | 1 | dx.doi.org/10.17504/protocols.io.n92ldmbrol5b/v1 | https://www.protocols.click/view/open-field-test-to-assess-spontaneous-locomotion-b-cvfxw3pn | natalia.lopezgonzalezdelrey, Zachary Gaertner | TITLE: Open Field test to assess spontaneous locomotion behavior in parkinsonian mice
AUTHORS: natalia.lopezgonzalezdelrey, Zachary Gaertner
[DESCRIPTION]
The Open Field task is a simple sensorimotor test used to determine general activity levels, gross locomotor activity, and exploration habits in rodent models of CN... | ["[Open Field Test] Total duration: 1 day", "[Protocol] Place the animal cage in the testing room to allow the animals to acclimate. 60 min", "[Protocol] Place the animal in the testing boxes. The animals are transferred to the center of a 40cm by 40cm box in the quiet dark room.", "[Protocol] Video record and record f... |
null | null | null | dx.doi.org/10.17504/protocols.io.uatesen | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes how to make an acoustic droplet ejection compatible plate for liquid handling on the Echo 555 using an epMotion 5075. The protocol expects (4) 96-well primer plates and an empty 384-well low dead volume (LDV) echo plate. The 4 plates will be compressed in... | ["[Prepare primer plates] Thaw and centrifuge primer plates.", "[Prepare primer plates] Appropriately label destination plate.", "[Setup epMotion automation platform] {\"blocks\":[{\"key\":\"3cngr\",\"text\":\"\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"3j7p... |
25,111 | RNA Isolation from Plant Tissue Protocol 12: Hot Acid Phenol Method for Angiosperms | null | dx.doi.org/10.17504/protocols.io.4rxgv7n | null | null | TITLE: RNA Isolation from Plant Tissue Protocol 12: Hot Acid Phenol Method for Angiosperms
AUTHORS:
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Implemented by: Sarah Covshoff, Rowan Sage and Julian Hibberd</div><div class = "text-block">This RNA isolation method is a multi-component method inv... | ["Add of saturated acid phenol (pH 4.3) and of RNA extraction buffer to a 15 ml snap cap tube. Warm tube to in a fume hood using a heat block.\n3 ml\n3 ml\n65 °C", "Homogenize tissue in liquid nitrogen using a mortar and pestle.", "Transfer up to of ground tissue to a tube containing the phenol-buffer mixture and c... |
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