id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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90,827 | Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) | 1 | dx.doi.org/10.17504/protocols.io.14egn3nrml5d/v1 | https://www.protocols.io/view/liquid-chromatography-with-tandem-mass-spectrometr-c4xjyxkn | Leonardo A Parra-Rivas | TITLE: Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
[STEPS]
1. The data acquisition by LC-MS/MS was adapted from a published procedure
2. Derived peptides were resuspended in a loa... | ["The data acquisition by LC-MS/MS was adapted from a published procedure", "Derived peptides were resuspended in a loading buffer\n(0.1% trifluoroacetic acid, TFA) and separated on a Water's Charged Surface\nHybrid (CSH) column (150 µm internal diameter x 15 cm; particle size: 1.7 µm).", "The samples were run on an EV... |
30,469 | High-Performance Liquid Chromatography (HPLC) | 1 | null | https://www.protocols.io/view/high-performance-liquid-chromatography-hplc-9zdh726 | Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying | TITLE: High-Performance Liquid Chromatography (HPLC)
AUTHORS: Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes Hig... | ["[HPLC Preparation]\nReplace neuron culture medium with Hanks’ balanced saline solution buffer with the addition of .\n[KCl]", "[HPLC Preparation]\nIncubate for at .\n37 °C", "[HPLC Preparation]\nCollect media and centrifuge to clear cell debris. Collect neuron pellet.", "[HPLC Preparation]\nFreeze immediately and st... |
27,269 | One Part CPEC and quick change | null | dx.doi.org/10.17504/protocols.io.6vdhe26 | null | N.J. Hillson | TITLE: One Part CPEC and quick change
AUTHORS: N.J. Hillson
[STEPS]
?. Setup 2 PCR-like reactions, each with only one of the two full-length primers:Sterile water 13.5 µLGC buffer 5 µLDMSO (30% stock) 2.5 µLdNTPs (10 mM stock) 0.5 µLPrimer (10 µM stock) 2.5 µLMini-prepped template DNA 0.5 µLPolymerase (Pfu) 0.5 µLTota... | ["Setup 2 PCR-like reactions, each with only one of the two full-length primers:Sterile water 13.5 µLGC buffer 5 µLDMSO (30% stock) 2.5 µLdNTPs (10 mM stock) 0.5 µLPrimer (10 µM stock) 2.5 µLMini-prepped template DNA 0.5 µLPolymerase (Pfu) 0.5 µLTotal 25 µL", "Combine both reactions and add 1 µL more of polymerase.", "... |
68,935 | APOE Genotyping Using TaqMan + Data Processing | 1 | dx.doi.org/10.17504/protocols.io.n92ldzo68v5b/v1 | https://www.protocols.io/view/apoe-genotyping-using-taqman-data-processing-cfjftkjn | Mario A Mosqueda | TITLE: APOE Genotyping Using TaqMan + Data Processing
AUTHORS: Mario A Mosqueda
[DESCRIPTION]
Protocol for APOE4 genotyping for SNPs rs7412 (C/T) and rs429358 (C/T)
[STEPS]
SECTION: Thaw DNA Samples
1. Thaw DNA samples and APOE Controls at room temp, gently vortex and briefly centrifuge to collect the DNA solution i... | ["[Thaw DNA Samples] Thaw DNA samples and APOE Controls at room temp, gently vortex and briefly centrifuge to collect the DNA solution into the bottom of the tube or well-plate..", "[Thaw DNA Samples] While the samples thaw, label a 96 well PCR plate \"DNA Dilution\"", "[Thaw DNA Samples] Dilute DNA in UP H2O (Sample a... |
26,704 | SPARC_Duke_PelotGrill_OT2-OD025340_PigVagusNerve_Collection_Histology_Microscopy | 1 | dx.doi.org/10.17504/protocols.io.6bqhamw | https://www.protocols.io/view/sparc-duke-pelotgrill-ot2-od025340-pigvagusnerve-c-6bqhamw | J. Ashley Ezzell, Nicole A. Pelot, Kara A. Clissold, Warren M. Grill | TITLE: SPARC_Duke_PelotGrill_OT2-OD025340_PigVagusNerve_Collection_Histology_Microscopy
AUTHORS: J. Ashley Ezzell, Nicole A. Pelot, Kara A. Clissold, Warren M. Grill
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for collection, histological processing (paraffin embedding, Masson's trichro... | ["[Collect pig vagus nerve samples.]\nWe collected fresh vagus nerve samples from Yorkshire pigs after they were euthanized following medical training courses at Duke University.", "[Collect pig vagus nerve samples.]\nWe collected samples of the carotid sheath in the neck bilaterally. We collected 2 cm samples at the l... |
null | null | null | dx.doi.org/10.17504/protocols.io.fp9bmr6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span class="TextRun SCX27213965" lang="EN-US" style="font-size: 14pt; font-family: Cambria,serif; line-height: 24px;" xml:lang="EN-US"><span class="NormalTextRun SCX27213965" style="background-color: inherit;">Procedure</span></span><span class="TextRun SCX27213965" lang="EN... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gs2bwge | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol for Northern Blot using radioactive RNA probes.</p>
[BEFORE_START]
<p>Generate a PCR product with a T7 promoter for generating riboprobes. Make sure the riboprobe is antisense to the RNA of interest.</p>
[STEPS]
?.
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108,203 | Isolation of high quality RNA from tree leaves using Polyclar in the Spectrum Plant Total RNA Kit | 0 | dx.doi.org/10.17504/protocols.io.14egn6qkml5d/v1 | https://www.protocols.io/view/isolation-of-high-quality-rna-from-tree-leaves-usi-dmwj47cn | Tobias Bruegmann, Franziska Orgel, Susanne Jelkmann, Hilke Schroeder | TITLE: Isolation of high quality RNA from tree leaves using Polyclar in the Spectrum Plant Total RNA Kit
AUTHORS: Tobias Bruegmann, Franziska Orgel, Susanne Jelkmann, Hilke Schroeder
[DESCRIPTION]
This protocol is based on the use of the Spectrum Plant Total RNA Kit from Sigma. The protocol has been adapted for the is... | ["[Sample preparation] Harvest plant material and freeze it immediately in liquid nitrogen. Alternatively, you can use frozen plant material.", "[Sample preparation] Grind approximately 100 mg using a swing mill (2-3 x 1:15 min at 17.5 Hz) with small metal balls or using a bead ruptor (3 × 15 s at 2.1 m/s).", "[Prepara... |
96,757 | Performance and Safety of Vaginal Administration of Tocopherol Acetate (Vitamin E) in Pre-, Peri-, and Post-Menopausal Women | 0 | dx.doi.org/10.17504/protocols.io.81wgbxmmqlpk/v1 | https://www.protocols.io/view/performance-and-safety-of-vaginal-administration-o-daqv2dw6 | Prof. Francesco Cosentino, Prof. Roberto Di Marco | TITLE: Performance and Safety of Vaginal Administration of Tocopherol Acetate (Vitamin E) in Pre-, Peri-, and Post-Menopausal Women
AUTHORS: Prof. Francesco Cosentino, Prof. Roberto Di Marco
[DESCRIPTION]
The vaginal microbiota has a significant bearing on a woman's mental and physical health at every stage of life. A... | [] |
48,743 | PCLS Single Cell Suspension | 4 | null | https://www.protocols.io/view/pcls-single-cell-suspension-btufnntn | Morrisey Lab | TITLE: PCLS Single Cell Suspension
AUTHORS: Morrisey Lab
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">PCLS Single Cell Suspension</span></div></div>
[STEPS] | [] |
94,179 | Copy number variation analysis by ddPCR | 4 | dx.doi.org/10.17504/protocols.io.q26g7pkd8gwz/v1 | https://www.protocols.io/view/copy-number-variation-analysis-by-ddpcr-c78bzrsn | Katie Jing Kay Lam, Olubankole Arogundade, Claire D Clelland | TITLE: Copy number variation analysis by ddPCR
AUTHORS: Katie Jing Kay Lam, Olubankole Arogundade, Claire D Clelland
[DESCRIPTION]
This protocol describes copy number variation analysis using Bio-Rad Droplet Digital PCR ddPCR and QuantaSoft Software (with modifications).
[STEPS]
SECTION: ddPCR reaction set up
2. Thaw... | ["[ddPCR reaction set up] Thaw all components to room temperature", "[Droplet generation] Insert DG8‱ Cartridge into cartridge holder", "[Droplet generation] FIRST - Load >20µL sample into wells\nNote:\n- Add a 50:50 mix of Supermix & H2O in empty wells\n- All 8 wells must be loaded with samples or Supermix + H2O\n- Ma... |
101,443 | Mapping ONT Reads to Reference Sequences with Minimap2 and Samtools | 0 | dx.doi.org/10.17504/protocols.io.4r3l2q98jl1y/v1 | https://www.protocols.io/view/mapping-ont-reads-to-reference-sequences-with-mini-dfbb3iin | Hung Luong, Hiep Vu | TITLE: Mapping ONT Reads to Reference Sequences with Minimap2 and Samtools
AUTHORS: Hung Luong, Hiep Vu
[DESCRIPTION]
This simple protocol maps ONT raw reads to Reference Sequences by Minimap2 and Samtools.
[STEPS]
SECTION: Install requirement software
1. git clone https://github.com/lh3/minimap2
cd minimap2 && make
... | ["[Install requirement software] git clone https://github.com/lh3/minimap2\ncd minimap2 && make", "[Install requirement software] conda create -n samtools\nconda activate samtools\nconda config --add channels bioconda\nconda config --add channels conda-forge\nconda install -c bioconda samtools", "[Align reads to refere... |
null | null | null | dx.doi.org/10.17504/protocols.io.shieb4e | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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?.
?.
?. | ["Third instar larvae were ground for 10 s in 100 µL of ice‐cold homogenization buffer (50 mm phosphate buffer containing 0.05% Tween‐80) using a Kontes pellet pestle motor.", "Homogenates were then diluted with an additional 500 µL of homogenization buffer.", "To remove cell debris, samples were centrifuged at 2000 g ... |
null | null | null | dx.doi.org/10.17504/protocols.io.i32cgqe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A non-invasive PCR-based methodology for sensitive detection and identification of parasitic nematode DNA released in the faeces of infected amphibians and reptiles as egg or tissue fragments (environmental DNA). A DNA extraction protocol optimised for liberation of DNA from ... | [] |
97,805 | Rotarod Test | 0 | dx.doi.org/10.17504/protocols.io.8epv5r6qjg1b/v1 | https://www.protocols.io/view/rotarod-test-dbrm2m46 | Sabina Marciano, Roberta Marongiu | TITLE: Rotarod Test
AUTHORS: Sabina Marciano, Roberta Marongiu
[DESCRIPTION]
Behavioral assay to measure motor impairments, specifically balance and coordination, in mice.
[STEPS]
1. Set up rotarod equipment as needed and clean thoroughly.
2. Turn on rotarod using green button and log into computer. Open software dir... | ["Set up rotarod equipment as needed and clean thoroughly.", "Turn on rotarod using green button and log into computer. Open software directly from desktop once machine is ON and connected.", "Specify settings:\na. Protocol: Increasing speed\nb. Speed: 4.0- 40 RPM\nc. File name [EXP NAME].txt \n... |
27,331 | “O-map/way method-taxa”: A sampling method for complete plant taxa inventories in large forests in the moderate/colline zone using orienteering maps or maps of a similar quality | null | dx.doi.org/10.17504/protocols.io.6xbhfin | null | André Strauss | TITLE: “O-map/way method-taxa”: A sampling method for complete plant taxa inventories in large forests in the moderate/colline zone using orienteering maps or maps of a similar quality
AUTHORS: André Strauss
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><ol style = "list-style-type: decimal;"><li... | ["Making 3 complete seasonal inspection rounds (feb/mar; may/jun; aug/sep) in the same year, ideally carried out by one person, in the forest target area using an orienteering map or a map of similar quality (in paper or in an electronic device; ideally at a scale of 1: 5 000) with additional means, such as GPS, compas... |
26,534 | U Michigan - Tail Cuff Blood Pressure Determination | null | dx.doi.org/10.17504/protocols.io.56eg9be | null | Jeff Hodgin | TITLE: U Michigan - Tail Cuff Blood Pressure Determination
AUTHORS: Jeff Hodgin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span><span style = "font-weight:bold;"> </span></div><div class = "text-block">This protocol provides information for the non-in... | ["Setting up Equipment and Software\n1.1 Turn on warming platforms. The ideal temperature for blood pressure (BP) monitoring is 30 ~ 35 ̊C. If the mouse tail is too cold, BP will not be read.However, if the warming platform is too hot, mice will be stressed and may lead to severe dehydration or even death.\n1.2 Selec... |
49,857 | LC-MS/MS quantitation of insulin, glucagon, somatostatin, and C-peptide | 3 | dx.doi.org/10.17504/protocols.io.buw9nxh6 | https://www.protocols.io/view/lc-ms-ms-quantitation-of-insulin-glucagon-somatost-buw9nxh6 | Matthew J. Donohue, Robert T. Filla, Daniel J. Steyer, Wesley J. Eaton, Michael G. Roper | TITLE: LC-MS/MS quantitation of insulin, glucagon, somatostatin, and C-peptide
AUTHORS: Matthew J. Donohue, Robert T. Filla, Daniel J. Steyer, Wesley J. Eaton, Michael G. Roper
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">An LC-MS method using multiple reaction monitoring for quantitation of ins... | [] |
82,320 | Fluorescence in situ hybridization (FISH) | 1 | dx.doi.org/10.17504/protocols.io.kqdg39mmeg25/v1 | https://www.protocols.io/view/fluorescence-in-situ-hybridization-fish-cumqwu5w | Peter Sims | TITLE: Fluorescence in situ hybridization (FISH)
AUTHORS: Peter Sims
[DESCRIPTION]
Fluorescence in situ hybridization (FISH) mRNA
[STEPS]
1. Mouse brain sections were prepared, removed from cryoprotectant solution, and washed three times in tris-buffered saline (TBS) at room temperature.
Sections were incubated with... | ["Mouse brain sections were prepared, removed from cryoprotectant solution, and washed three times in tris-buffered saline (TBS) at room temperature. \nSections were incubated with hydrogen peroxide (ACD) for 15 min at room temperature, washed several times in TBS, and then mounted to Superfrost slides. \nSections were... |
27,335 | Vandy - Myocardial Ischemia Reperfusion | null | dx.doi.org/10.17504/protocols.io.6xfhfjn | null | Lin Zhong, Jeffrey Rottman, Chee Lim | TITLE: Vandy - Myocardial Ischemia Reperfusion
AUTHORS: Lin Zhong, Jeffrey Rottman, Chee Lim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">The most common cause of cardiovascular mortality in man is the outcome from m... | ["Mice are anesthetized with pentobarbital (50 mg.kg, IP).", "The ventral neck and left parasternal region is shaved and disinfected with Betadine followed by 70% alcohol.", "The mouse is positioned supineon a heating pad and a small incision is made through the skin underlying the trachea.", "The trachea is exposed, a... |
19,115 | Derivation of Gnotobiotic Stickleback Fish | 1 | dx.doi.org/10.17504/protocols.io.n2bvjrd6plk5/v1 | https://www.protocols.io/view/derivation-of-gnotobiotic-stickleback-fish-wwjffcn | Kat Milligan-McClellan | TITLE: Derivation of Gnotobiotic Stickleback Fish
AUTHORS: Kat Milligan-McClellan
[DESCRIPTION]
This protocol is used to generate gnotobiotic stickleback fish. Based on the zebrafish protocols, this was optimized for stickleback. See the paper https://doi.org/10.1242/dmm.021881 for the first description of the use of ... | ["[Fertilize eggs] Put on gloves", "[Fertilize eggs] Gently squeeze eggs from females into sterile 20 or 60mm petri dishes. Place the cover on the eggs and transfer to the dissection area.", "[Fertilize eggs] Clean the surface of the dissection area with 70% ethanol", "[Fertilize eggs] Euthanize the male in freshly mad... |
null | null | null | dx.doi.org/10.17504/protocols.io.hixb4fn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<p>This protocol describes the immunostaining of cultured rat hippocampal neurons to stain for ratFwe2 in cell body. It is from 'Flower Ca<sup>2+</sup> channel in CME and ADBE' of Yao CK et al.</p>
<p> </p>
<p>Please see the manuscript for the full method details.</p>
</di... | [] |
79,543 | Complex I activity assay | 4 | dx.doi.org/10.17504/protocols.io.4r3l27r8qg1y/v1 | https://www.protocols.io/view/complex-i-activity-assay-crwxv7fn | María José Pérez J., michela.deleidi | TITLE: Complex I activity assay
AUTHORS: María José Pérez J., michela.deleidi
[DESCRIPTION]
This protocol describes the complex I activity assay.
[BEFORE_START]
All assays are carried out at 25 °C.
After mitochondrial isolation (Qproteome Mitochondria Isolation Kit. QIAGEN Cat. No. / ID: 37612), resuspend the final p... | ["[Protocol] Distribute the contents of tube A and B in strips suitable for multichannel use.", "[Protocol] In a Half Volume 96-well clear plate add 50 µL of the contents of tube A to each well.", "[Protocol] Add 20 µL of sample to each well.", "[Protocol] Place plate in plate reader and add 30 µL of B to each well.", ... |
null | null | null | dx.doi.org/10.17504/protocols.io.jtxcnpn | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
54,232 | Augur/Auspice/UShER SARS-CoV-2 Cluster Detection Workflow for the Terra Platform | 5 | null | https://www.protocols.io/view/augur-auspice-usher-sars-cov-2-cluster-detection-w-by7ypzpw | Anusha Ginni, Jill V Hagey, Michael Weigand, Technical Outreach and Assistance for States Team | TITLE: Augur/Auspice/UShER SARS-CoV-2 Cluster Detection Workflow for the Terra Platform
AUTHORS: Anusha Ginni, Jill V Hagey, Michael Weigand, Technical Outreach and Assistance for States Team
[DESCRIPTION]
The Titan_Augur workflow processes concatenated assembly fasta data for SARS-CoV-2 phylogenetic analysis and ... | ["[Setup Terra and Google Cloud Accounts]", "[Import Augur workflows from Dockstore] Importing the Titan_Augur Workflows from Dockstore to the Terra User Workspace\n\nNote: The Augur workflow includes two steps: Titan_Augur_Prep and Titan_Augur_Run.\n\nThe Titan_Augur_Prep workflow prepares the metadata and assembly fa... |
103,786 | Assessing Precision of One’s Own Pipetting | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1okzlmk/v1 | https://www.protocols.io/view/assessing-precision-of-one-s-own-pipetting-dhki34ue | Jonathan Phillips, Gregor Blaha | TITLE: Assessing Precision of One’s Own Pipetting
AUTHORS: Jonathan Phillips, Gregor Blaha
[DESCRIPTION]
Accurate pipetting is critical for producing precise in vitro assay results. However, since pipetting is such a routine task, many researchers believe their pipetting is precise enough. Rarely do they critically ev... | [] |
34,763 | Dissolved sulfide concentrations (H2S, HS-, S2-) colorimetric assay using a plate reader (96-well plate) | 1 | null | https://www.protocols.io/view/dissolved-sulfide-concentrations-h2s-hs-s2-colorim-bd7ji9kn | Jian Gong | TITLE: Dissolved sulfide concentrations (H2S, HS-, S2-) colorimetric assay using a plate reader (96-well plate)
AUTHORS: Jian Gong
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the adaption of a sulfide colorimetric assay originally described in </span><a href="https:... | ["Sample stabilization: Add 400 µL of liquid sample in 1 ml of reagent A upon sampling. This step makes zinc sulfide and stabilizes the sample from further oxidation (should be already done during sampling). Here 400 µL corresponds roughly to a detection range at 3 - 40 µM sulfide-sulfur. This amount can be further red... |
27,406 | Isolation of Nuclei from Frozen Human Dorsal Root Ganglia | null | dx.doi.org/10.17504/protocols.io.6znhf5e | null | Alexander Chamessian | TITLE: Isolation of Nuclei from Frozen Human Dorsal Root Ganglia
AUTHORS: Alexander Chamessian
[STEPS]
?. [Cryo-pulverization] | ["[Cryo-pulverization]"] |
null | null | null | dx.doi.org/10.17504/protocols.io.e2pbgdn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Anti-BrdU Staining Protocol Using 70% Ethanol and 2N HCl</p>
[STEPS]
?.
?.
?.
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?.
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62,110 | Keto Start ACV {2022} – Benefits & Side Effects! Is It helpful For Easy Weight Loss? | 3 | dx.doi.org/10.17504/protocols.io.6qpvr6mnbvmk/v1 | https://www.protocols.io/view/keto-start-acv-2022-benefits-amp-side-effects-is-i-b8v6rw9e | Danieldevines | TITLE: Keto Start ACV {2022} – Benefits & Side Effects! Is It helpful For Easy Weight Loss?
AUTHORS: Danieldevines
[DESCRIPTION]
Keto Start ACV can likewise be applied as a wellspring of electricity and a craving suppressant. These chewy sweets are simply handy for purchase at the authority site. Many people tak... | [] |
34,619 | Radioactive Labeling with T4 PNK 3' phosphatase minus (M0236) | 1 | dx.doi.org/10.17504/protocols.io.bd23i8gn | https://www.protocols.io/view/radioactive-labeling-with-t4-pnk-3-39-phosphatase-bd23i8gn | New England Biolabs | TITLE: Radioactive Labeling with T4 PNK 3' phosphatase minus (M0236)
AUTHORS: New England Biolabs
[DESCRIPTION]
Radioactive Labeling with T4 Polynucleotide Kinase 3' phosphatase minus.
[STEPS]
1. Set-up the following reaction:
2. Incubate at 37 °C for 30 min.
3. Heat inactivate by incubating at 65 °C... | ["Set-up the following reaction:", "Incubate at 37 °C for 30 min.", "Heat inactivate by incubating at 65 °C for 20 min."] |
null | null | null | dx.doi.org/10.17504/protocols.io.sqvedw6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Aims: Stress granules (SGs) are transient cytoplasmic mRNA and protein complexes that form in eukaryotic cells under stress. SGs are related to multiple diseases, but there are no reports of the existence of SGs in atrial fibrillation (AF); hence, the effects of SGs in AF are... | [] |
40,897 | ELISA for quantification of tumor necrosis factor alpha (TNF-α) in human serum or plasma. | 6 | dx.doi.org/10.17504/protocols.io.bj69krh6 | https://www.protocols.io/view/elisa-for-quantification-of-tumor-necrosis-factor-bj69krh6 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme | TITLE: ELISA for quantification of tumor necrosis factor alpha (TNF-α) in human serum or plasma.
AUTHORS: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Tumor necrosis factor</span><span> (</span><span style = "fo... | ["An anti-human TNF-α coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.", "Add 50 µl of human serum or plasma. Human TNF-α present in the serum or plasma binds to antibodies adsorbed into the microwells.", "The microplate is blocked with 3% non-fat milk-P... |
79,397 | DNA isolation from cattle semen for long read sequencing | 4 | dx.doi.org/10.17504/protocols.io.j8nlkw1qwl5r/v1 | https://www.protocols.io/view/dna-isolation-from-cattle-semen-for-long-read-sequ-crsdv6a6 | Erwan Denis, Cecile CG Grohs, Carole Iampietro | TITLE: DNA isolation from cattle semen for long read sequencing
AUTHORS: Erwan Denis, Cecile CG Grohs, Carole Iampietro
[DESCRIPTION]
Here we describe a method for isolate high molecular weight DNA from commercially available frozen bull semen straws.
This protocol is based on a salting-out method and uses several co... | ["[Preparation of sample] Recovery of spermatozoa from the straw:\n- Empty the 200 µL in a 2 mL by cutting the two ends of the straw\n\n \n\n- Rince the straw it with 200 µL Room temperature", "[Lysis] Step one: \n\nAdd 500 µL of RLT-TCEP to the pellet\n- Vortex 10 s by pulsing at max speed\n- If necessary, use a wi... |
48,099 | A lateral flow-based at-home test for detection of SARS-CoV-2 | 1 | dx.doi.org/10.17504/protocols.io.bs8bnhsn | https://www.protocols.io/view/a-lateral-flow-based-at-home-test-for-detection-of-bs8bnhsn | Peng Xu, Venice Servellita, Krzysztof Langer, Dan Weisgerber, Gordon Murtaugh, Adam R Abate, Charles Chiu | TITLE: A lateral flow-based at-home test for detection of SARS-CoV-2
AUTHORS: Peng Xu, Venice Servellita, Krzysztof Langer, Dan Weisgerber, Gordon Murtaugh, Adam R Abate, Charles Chiu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">We have developed a highly sensitive and specific LAMP(loop-mediated... | ["[QUALITY CONTROL]\n1. Positive control (PC) – pooled positive samples aliquoted into single-use tubes with 80μL/tube andstored in -80˚C until needed (prepared at UCSF).a. Multiple freeze/thaw cycles should be avoided. Maintain on ice when thawed.2. Negative template control (NTC) – nuclease-free water aliquoted into ... |
86,818 | Collection, Broadcast, Packaging and Shipping of Non-Islet Biospecimens | 4 | dx.doi.org/10.17504/protocols.io.81wgby71nvpk/v2 | https://www.protocols.io/view/collection-broadcast-packaging-and-shipping-of-non-cy2axyae | Integrated Islet Distribution Program | TITLE: Collection, Broadcast, Packaging and Shipping of Non-Islet Biospecimens
AUTHORS: Integrated Islet Distribution Program
[DESCRIPTION]
This SOP defines a standardized method for processing, broadcasting, packaging and shipping preparation of research quality non-islet biospecimens. These tissues may be matched o... | ["[Collection of Acinar/Non-Islet Pancreatic (Acinar) Tissue (NIPT): for Distribution (Fresh and Frozen)] The NIPT (the tissue remaining in the COBE purification bag) should be transferred under sterile conditions, into two 250 mL conical tubes, as soon as feasible during the islet isolation process. Top with HBSS, c... |
35,282 | Artificial Cerebrospinal Fluid IV (ACSF.IV) | null | dx.doi.org/10.17504/protocols.io.bepsjdne | null | Allen Institute for Brain Science | TITLE: Artificial Cerebrospinal Fluid IV (ACSF.IV)
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes making Artificial Cerebrospinal Fluid IV (ACSF.IV). ACSF.IV is used for multiple applications including incubation of fresh mouse brai... | [] |
72,354 | ReverseTranscription-Protocol-miRNA-SCALONMC | 6 | dx.doi.org/10.17504/protocols.io.261ge33j7l47/v1 | https://www.protocols.io/view/reversetranscription-protocol-mirna-scalonmc-ciwaufae | Marcela Scalon, Christine Souza Martins, Gabriel Ginani Ferreira, Franciele Schlemmer, Ricardo Titze de Almeida, Giane Regina Paludo | TITLE: ReverseTranscription-Protocol-miRNA-SCALONMC
AUTHORS: Marcela Scalon, Christine Souza Martins, Gabriel Ginani Ferreira, Franciele Schlemmer, Ricardo Titze de Almeida, Giane Regina Paludo
[DESCRIPTION]
This protocol is intended as a guideline to perform the Reverse Transcription procedure to detect and quantify ... | ["[Prepare de RT mix] In a polypropylene 0,6 tube, prepare the RT mix on ice by scaling the volumes listed below to the desired number of RT reactions + 1 (excess volume to compensate for losses that occur during pipetting):", "Mix gently. Centrifuge to bring the solution to the bottom of the tube.", "Place the RT mix ... |
null | null | null | dx.doi.org/10.17504/protocols.io.pnwdmfe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Adapted from Qiagen's <a href="https://www.qiagen.com/us/resources/resourcedetail?id=d2b85b26-16dd-4259-a3a7-a08cbd2a08a3&lang=en" target="_blank" rel="noopener noreferrer">genomic DNA handbook</a> and the <a href="https://www.qiagen.com/us/resources/resourcedetail?id=b45... | [] |
39,440 | Low-input DNA extractions in 96-well plates | 1 | dx.doi.org/10.17504/protocols.io.birqkd5w | https://www.protocols.io/view/low-input-dna-extractions-in-96-well-plates-birqkd5w | Tom Harrop, Reuben McKay Vercoe, Ciarán Cuddy | TITLE: Low-input DNA extractions in 96-well plates
AUTHORS: Tom Harrop, Reuben McKay Vercoe, Ciarán Cuddy
[STEPS]
?. [Prepare lysate for extraction]
Prepare lysate for extraction
?. [Prepare lysate for extraction]
Dissect e.g. 1 M. hyperodae pupa for a single sample.Place up to 88 individuals in a 1000 µL, round well ... | ["[Prepare lysate for extraction]\nPrepare lysate for extraction", "[Prepare lysate for extraction]\nDissect e.g. 1 M. hyperodae pupa for a single sample.Place up to 88 individuals in a 1000 µL, round well deepwell plateAdd 2 3.2 mm stainless steel ball bearings and 100 µL of lysis buffer with RNase to each well.", "[P... |
84,312 | Symbiotic Dose-50 (SD50) for Vibrio fischeri strain to colonize Euprymna scolopes | 4 | dx.doi.org/10.17504/protocols.io.yxmvm2155g3p/v2 | https://www.protocols.click/view/symbiotic-dose-50-sd50-for-vibrio-fischeri-strain-cwjyxcpw | ard, ejg, agc, Tim I Miyashiro | TITLE: Symbiotic Dose-50 (SD50) for Vibrio fischeri strain to colonize Euprymna scolopes
AUTHORS: ard, ejg, agc, Tim I Miyashiro
[DESCRIPTION]
This protocol details symbiotic dose-50 (SD50) for Vibrio fischeri strain to colonize Euprymna scolopes.
[STEPS]
SECTION: Preparation of V. fischeri Cultures
1. For each strai... | ["[Preparation of V. fischeri Cultures] For each strain of interest, initiate a starter culture by inoculating 3 mL LBS with an isolated colony. Incubate starter cultures (~16 h) at 28 °C shaking at .", "[Preparation of V. fischeri Cultures] Measure the OD600 of each starter culture. In a microfuge tube, normalize ... |
63,857 | {Exposed} Keto Now Reviews Ketosis and Scam Keto Complete Pills – Test and Buy! | 3 | dx.doi.org/10.17504/protocols.io.81wgb69bolpk/v1 | https://www.protocols.io/view/exposed-keto-now-reviews-ketosis-and-scam-keto-co-cakrscv6 | keto-x-reviews | TITLE: {Exposed} Keto Now Reviews Ketosis and Scam Keto Complete Pills – Test and Buy!
AUTHORS: keto-x-reviews
[DESCRIPTION]
Keto Now Reviews
[STEPS] | [] |
80,973 | Rigour of Development of European Society of Cardiology, American College of Cardiology and American Heart Association guidelines over a 10-year period (2013-2022): A systematic review of guidelines. | 1 | dx.doi.org/10.17504/protocols.io.j8nlkwq6xl5r/v1 | https://www.protocols.io/view/rigour-of-development-of-european-society-of-cardi-ctbmwik6 | r.providencia | TITLE: Rigour of Development of European Society of Cardiology, American College of Cardiology and American Heart Association guidelines over a 10-year period (2013-2022): A systematic review of guidelines.
AUTHORS: r.providencia
[DESCRIPTION]
This protocol describes in the detail the processes for a systematic review... | ["[Systematic Review ID and Affiliation] Review title\nRigour of Development of European Society of Cardiology, American College of Cardiology and American Heart Association guidelines over a 10-year period (2013-2022): A systematic review of guidelines.", "[Systematic Review ID and Affiliation] Original language title... |
18,707 | NASC-seq (new transcriptome alkylation-dependent single-cell RNA sequencing) protocol | null | dx.doi.org/10.17504/protocols.io.whtfb6n | null | Gert-Jan Hendriks, Lisa Anna Jung, Anton J.M. Larsson, Oscar Andersson Forsman, Michael Lidschreiber, Katja Lidschreiber, Patrick Cramer, Rickard Sandberg | TITLE: NASC-seq (new transcriptome alkylation-dependent single-cell RNA sequencing) protocol
AUTHORS: Gert-Jan Hendriks, Lisa Anna Jung, Anton J.M. Larsson, Oscar Andersson Forsman, Michael Lidschreiber, Katja Lidschreiber, Patrick Cramer, Rickard Sandberg
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [P... | ["[Prepare lysis plate]\nPrepare the following lysis buffer on ice. ABC1Reagent1 reaction96-well plate2Sodium Phosphate buffer (200mM)2.5 μl264 μl3RNAse inhibitor0.2 μl21.12 μl4ERCC + 4sU Spike-in mix0.1 μl10.56 μl5Nuclease-free water0.2 μl21.12 μl6Total3 μl316.8 μl\nABC1Reagent1 reaction96-well plate2Sodium Phosphate... |
99,976 | Intramuscular Injection Adult Mouse | 1 | dx.doi.org/10.17504/protocols.io.n2bvjybqbvk5/v2 | https://www.protocols.io/view/intramuscular-injection-adult-mouse-ddvg263w | Allen Institute for Brain Science | TITLE: Intramuscular Injection Adult Mouse
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
This protocol describes the general procedures used for intramuscular injection in adult mice.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Inst... | [] |
33,844 | Japanese encephalitis virus isolation | 1 | dx.doi.org/10.17504/protocols.io.bdaui2ew | https://www.protocols.io/view/japanese-encephalitis-virus-isolation-bdaui2ew | Wenjing Liu, Shihong Fu | TITLE: Japanese encephalitis virus isolation
AUTHORS: Wenjing Liu, Shihong Fu
[DESCRIPTION]
The mosquito grinding supernatant and CSF samples were inoculated into Vero cells to finish virus isolation.
[BEFORE_START]
Mosquito grinding supernatant was sterilized using a filter with a pore size of 0.22 μm.
[STEPS]
SEC... | ["[virus isolation] The filtered sterile mosquito grinding solution was inoculated into 24-well plates grown to 80% monolayer Vero cells, 70 μl of each well was added for virus isolation, and a 3-well blank control was set for each plate.(for CSF samples 100μl of each well was added for virus isolation).", "[virus isol... |
31,341 | Dissection and immunohistochemistry of mouse vagal ganglia | null | dx.doi.org/10.17504/protocols.io.baumieu6 | null | Thomas Taylor-Clark, Seol-Hee Kim | TITLE: Dissection and immunohistochemistry of mouse vagal ganglia
AUTHORS: Thomas Taylor-Clark, Seol-Hee Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Mice are euthanized, perfused with fixative and the vagal ganglia extracted. Vagal ganglia are then cyosectioned. Slices are stained for protei... | ["6 to 8 weeks old mice (male) were euthanized by CO2 inhalation.", "Mice were transcardially perfused with phosphate buffered saline (PBS) to remove blood followed by 3.7% formaldehyde (PFA) in PBS.", "Vagal ganglia (including both jugular and nodose ganglia) were collected and fixed with 3.7% PFA for 2 hours on ice."... |
46,652 | NMJ | 1 | dx.doi.org/10.17504/protocols.io.brs4m6gw | https://www.protocols.io/view/nmj-brs4m6gw | Jake Willows, Kristy Townsend, Magdalena Blaszkiewicz | TITLE: NMJ
AUTHORS: Jake Willows, Kristy Townsend, Magdalena Blaszkiewicz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A series of protocols to fluorescently label neuromuscular junctions in mice for qualitative and quantitative studies.</div></div>
[STEPS]
?. See attachments under Abstract for ... | ["See attachments under Abstract for PDF copies of protocols."] |
53,855 | High Resolution Ex Vivo Structural MRI Imaging & Analysis Protocol | 3 | null | https://www.protocols.io/view/high-resolution-ex-vivo-structural-mri-imaging-amp-byt7pwrn | Bruce Fischl, Andre Van Der Kouwe, Divya Varadarajan, Matthew Dylan Tisdall, Leah Morgan, Robert Frost, Allison Stevens | TITLE: High Resolution Ex Vivo Structural MRI Imaging & Analysis Protocol
AUTHORS: Bruce Fischl, Andre Van Der Kouwe, Divya Varadarajan, Matthew Dylan Tisdall, Leah Morgan, Robert Frost, Allison Stevens
[DESCRIPTION]
At the Martinos Center for Biomedical Imaging, we are continuously developing cutting-edge ... | [] |
47,134 | One-Step RT-ddPCR for Detection of SARS-CoV-2, Bovine Coronavirus, and PMMoV RNA in RNA Derived from Wastewater or Primary Settled Solids v2 | 1 | dx.doi.org/10.17504/protocols.io.br96m99e | https://www.protocols.io/view/one-step-rt-ddpcr-for-detection-of-sars-cov-2-bovi-br96m99e | Stephanie Loeb, Katy Graham, David Catoe, Marlene Wolfe, Alexandria Boehm, Krista Wigginton | TITLE: One-Step RT-ddPCR for Detection of SARS-CoV-2, Bovine Coronavirus, and PMMoV RNA in RNA Derived from Wastewater or Primary Settled Solids v2
AUTHORS: Stephanie Loeb, Katy Graham, David Catoe, Marlene Wolfe, Alexandria Boehm, Krista Wigginton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Th... | ["[Plan Analysis - Plate Setup]\nThe goal of this procedure is to quantify concentration of N1, N2, BCoV, and PMMoV in extractions using RT-ddPCR. Two duplexed assays will be run for each sample and are described in this protocol: 1) SARS-CoV-2 N1/N2, and 2) BCoV and PMMoV. Samples from a single plant should be run tog... |
82,596 | Multi-Proxy Sampling Protocol for Teeth from Archaeological Collections | 1 | dx.doi.org/10.17504/protocols.io.bp2l69o8rlqe/v1 | https://www.protocols.io/view/multi-proxy-sampling-protocol-for-teeth-from-archa-cuwcwxaw | Sarah Defant, Pavlína Ingrová, Denisa Zlámalová, Arkadiusz Sołtysiak, Zuzana Hofmanová | TITLE: Multi-Proxy Sampling Protocol for Teeth from Archaeological Collections
AUTHORS: Sarah Defant, Pavlína Ingrová, Denisa Zlámalová, Arkadiusz Sołtysiak, Zuzana Hofmanová
[DESCRIPTION]
This protocol introduces a streamlined approach for concurrent sampling for endogenous and microbial ancient DNA (aDNA) and stable... | ["[Decontamination & Pre-Sampling] General Remarks", "[Decontamination & Pre-Sampling] 1. All new samples in their original (sample) bags must be cleaned on all sides using diluted bleach and then UV-irradiated for30 min before further use. Be careful when cleaning the bags to avoid removing any sample informat... |
52,505 | Mary Berry CBD Gummies Reviews | 1 | dx.doi.org/10.17504/protocols.io.bxhzpj76 | https://www.protocols.io/view/mary-berry-cbd-gummies-reviews-bxhzpj76 | health | TITLE: Mary Berry CBD Gummies Reviews
AUTHORS: health
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Mary Berry CBD Gummies Reviews</div></div>
[STEPS]
?. Mary Berry CBD Gummies UK: Official Website!Mary Berry CBD Gummies are a scientifically proven product that effectively relieves and heals p... | ["Mary Berry CBD Gummies UK: Official Website!Mary Berry CBD Gummies are a scientifically proven product that effectively relieves and heals pain and stress in the body without causing harm to the consumer's health. Experts endorse these CBD-infused candies, claiming that they would provide optimum health advantage... |
null | null | null | dx.doi.org/10.17504/protocols.io.gzjbx4n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>PCR using Phusion HotStart II Hifi Mastermix (based on ThermoFisher Scientific manufacturer protocol).</p>
[STEPS]
?.
?.
?. | [] |
89,228 | Ex vivo Ca2+ 2PLSM measurements with genetically encoded probes | 4 | dx.doi.org/10.17504/protocols.io.5jyl8pby7g2w/v1 | https://www.protocols.io/view/ex-vivo-ca2-2plsm-measurements-with-genetically-en-c3dkyi4w | enrico.zampese | TITLE: Ex vivo Ca2+ 2PLSM measurements with genetically encoded probes
AUTHORS: enrico.zampese
[DESCRIPTION]
Ex-vivo slices obtained from mice that had previously undergone viral stereotaxic injections to express genetically encoded probes are used for fluorescence microscopy experiments. Because of the thickness of... | ["[Standard experimental procedure] Brain slices expressing GECI are obtained according to protocol and held at room temperature in a chamber containing aCSF continuously bubbled with 95% O2/5% CO2 blood gas mixture until the moment of the experiment.", "[Standard experimental procedure] Turn on 2PLSM working station, ... |
59,347 | Useful Methods 2: Sterilization of Duckweed | 4 | dx.doi.org/10.17504/protocols.io.eq2lynwzrvx9/v1 | https://www.protocols.io/view/useful-methods-2-sterilization-of-duckweed-b57tq9nn | Klaus-J. Appenroth | TITLE: Useful Methods 2: Sterilization of Duckweed
AUTHORS: Klaus-J. Appenroth
[DESCRIPTION]
This protocol details about sterilization of duckweed. It contains protocols from the The International Steering Committee on Duckweed Research and Application (ISCDRA) Newsletter. A complete list of these news letters can be ... | ["[For surface disinfection] Put the plants in a plastic tube (Falcon), depending on the size of the plants having a volume of 15 mL or 50 mL.", "[For surface disinfection] Dilute the commercial available “Eau de Javel” in water for different concentrations and treat the fronds for different periods by gentle shaking:... |
103,956 | Evaluating the Therapeutic Effects of Amino Acid Treatment on Vaso-Occlusive Pain in Sickle Cell Disease: A Systematic Review and Meta-Analysis Protocol | 0 | dx.doi.org/10.17504/protocols.io.kxygxyo5ol8j/v1 | https://www.protocols.io/view/evaluating-the-therapeutic-effects-of-amino-acid-t-dhru356w | Bohan Zhang, Ciaran Bubb, Sophie Yao, Vivian Dong, Priyal Patel, Aanya Shahani, Katie Lobner, Oluwakemi Badaki-Makun MD PhD | TITLE: Evaluating the Therapeutic Effects of Amino Acid Treatment on Vaso-Occlusive Pain in Sickle Cell Disease: A Systematic Review and Meta-Analysis Protocol
AUTHORS: Bohan Zhang, Ciaran Bubb, Sophie Yao, Vivian Dong, Priyal Patel, Aanya Shahani, Katie Lobner, Oluwakemi Badaki-Makun MD PhD
[DESCRIPTION]
Introduction... | ["[Abstract] Introduction: Sickle Cell Disease (SCD) affects over 100,000 individuals in the United States and 20 million globally, causing acute and chronic pain. The disease is characterized by misshapen red blood cells caused by mutations in beta-hemoglobin genes. SCD leads to multiorgan damage, chronic anemia, and ... |
22,746 | Role of Hyaluronic acid in evaluating tensile strengths of commonly used non absorbable suture materials | null | dx.doi.org/10.17504/protocols.io.2f2gbqe | null | Sudhir Varma, Salim Abufanas [Ajman University}, Mohamed Jaber, Vijay Desai | TITLE: Role of Hyaluronic acid in evaluating tensile strengths of commonly used non absorbable suture materials
AUTHORS: Sudhir Varma, Salim Abufanas [Ajman University}, Mohamed Jaber, Vijay Desai
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Abstract</div><div class = "text-block">Objective: In P... | ["Two non-absorbable suture materials were exposed to two different media (1 control and 1 test) in in vitro settings and thermostatically controlled environment. The suture materials were evaluated for tensile strength at pre immersion and later at 24hrs post immersion in selected medium.Study MaterialsTested suture m... |
40,402 | Enzyme linked immunosorbent assay for investigating the binding of chemically prepared protein-LAG-anti-IgY (SpLAG-anti-IgY) to avian and mammalian immunoglobulins. | 6 | dx.doi.org/10.17504/protocols.io.bjpskmne | https://www.protocols.io/view/enzyme-linked-immunosorbent-assay-for-investigati-bjpskmne | Angel Justiz-Vaillant, Norma McFarlane-Anderson | TITLE: Enzyme linked immunosorbent assay for investigating the binding of chemically prepared protein-LAG-anti-IgY (SpLAG-anti-IgY) to avian and mammalian immunoglobulins.
AUTHORS: Angel Justiz-Vaillant, Norma McFarlane-Anderson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SpLAG-anti-IgY... | ["This ELISA is used to study the interaction of protein-LAG-anti-IgY (SpLAG-anti-IgY) with different immunoglobulin preparations from avian and mammalian species.", "The 96 well microtitre plate is coated overnight at 4°C with 2 µg/µl per well of a mixture of SpL, SpA, SpG and anti-IgY (equal concentration of each pro... |
82,015 | Synthetic Leaching Procedure for Soils | 1 | dx.doi.org/10.17504/protocols.io.n2bvj8kdngk5/v1 | https://www.protocols.io/view/synthetic-leaching-procedure-for-soils-cub7wsrn | joshua.padilla | TITLE: Synthetic Leaching Procedure for Soils
AUTHORS: joshua.padilla
[DESCRIPTION]
This is a protocol following the EPA's Synthetic Leaching Procedure (SLP) for soils. This method provides an estimate of rainfall-leachable ions from soils, particularly from those that are metal-contaminated.
[STEPS]
SECTION: Prepar... | ["[Preparation of SLP acid mixture.] Add 2.4 g of H2SO4 and 1.6 g of HNO3 to about 30 mL of DI water in a graduated 50 mL centrifuge tube. Adjust the final volume to 40 mL. Add 1 mL of this to about 40 mL of DI water in a second graduated 50 mL centrifuge tube. Adjust the final volume to 50 mL. Wear proper PPE at all ... |
101,617 | Bacterial fluorescent in situ hybridization (FISH) in Ephydatia muelleri | 0 | dx.doi.org/10.17504/protocols.io.3byl49w52go5/v1 | https://www.protocols.io/view/bacterial-fluorescent-in-situ-hybridization-fish-i-dfgr3jv6 | Haley Womack, Gwendolyn Geiger, Scott Nichols | TITLE: Bacterial fluorescent in situ hybridization (FISH) in Ephydatia muelleri
AUTHORS: Haley Womack, Gwendolyn Geiger, Scott Nichols
[DESCRIPTION]
This protocol is used to visualize bacteria in and around gemmule-hatched freshwater sponges using a Eubacteria FISH probe.
[STEPS]
SECTION: Grow sponges
1. Add 3-4 mL ... | ["[Grow sponges] Add 3-4 mL mL of spring water to a 35mm coverslip bottom dish and place 1-3 gemmules into the center of the inner well.", "[Grow sponges] Let grow in the dark (to reduce autofluorescence of algae) at Room temperature for about 10080 min (1 week) or until tissue appears developed.", "[Day One] Make fixa... |
78,514 | Topographical distribution and morphology of SP-IR axons in the antrum, pylorus, and duodenum of mice | 1 | dx.doi.org/10.17504/protocols.io.e6nvwj41zlmk/v1 | https://www.protocols.io/view/topographical-distribution-and-morphology-of-sp-ir-cqwsvxee | Anas Mistareehi, Kohlton Bendowski, Ariege Bizanti, Jazune Madas, Yuanyuan Zhang, Andrew Kwiat, Duyen Nguyen, Nicole Kogut, Jichao Ma, Jin Chen, Zixi Cheng | TITLE: Topographical distribution and morphology of SP-IR axons in the antrum, pylorus, and duodenum of mice
AUTHORS: Anas Mistareehi, Kohlton Bendowski, Ariege Bizanti, Jazune Madas, Yuanyuan Zhang, Andrew Kwiat, Duyen Nguyen, Nicole Kogut, Jichao Ma, Jin Chen, Zixi Cheng
[DESCRIPTION]
This protocol describes the pr... | ["[Animals] 12 to 13-week old male were used. The animals were housed in a room in which light/dark cycles were set to 12 h/12 h (6:00 AM to 6:00 PM light cycle) and supplied with food and water ad libitum. Animals were fasted overnight, and food was provided 2h before perfusion. A catheter was introduced during per... |
81,807 | Pré-recrutamento das voluntárias | 1 | dx.doi.org/10.17504/protocols.io.q26g7yoz9gwz/v1 | https://www.protocols.io/view/pr-recrutamento-das-volunt-rias-ct5pwq5n | Alicia Rafaelly Vilefort Sales, Daniel Vartanian, Maria Augusta Medeiros Andrade, Alicia Rafaelly Vilefort Sales | TITLE: Pré-recrutamento das voluntárias
AUTHORS: Alicia Rafaelly Vilefort Sales, Daniel Vartanian, Maria Augusta Medeiros Andrade, Alicia Rafaelly Vilefort Sales
[DESCRIPTION]
Este protocolo foi desenhado para o processo de coleta de dados do projeto de pesquisa Sono e Gravidez. Consulte a aba Guidelines para visualiz... | ["[Preparo] Certifique que você está em um ambiente calmo, sem muitos ruídos e com acesso a uma internet estável.", "[Contato] Ligue para cada gestante .", "[Preparo] Por meio das informações presentes na agenda da Casa Parto, identifique e separe as informações das gestantes que estão com consulta de abertura de plano... |
92,325 | Harvesting and culturing of peritoneal macrophages and ex-vivo stimulation | 4 | dx.doi.org/10.17504/protocols.io.j8nlkoyoxv5r/v1 | https://www.protocols.io/view/harvesting-and-culturing-of-peritoneal-macrophages-c6edzba6 | Rebecca Wallings | TITLE: Harvesting and culturing of peritoneal macrophages and ex-vivo stimulation
AUTHORS: Rebecca Wallings
[DESCRIPTION]
Harvesting and culturing of peritoneal macrophages and ex-vivo stimulation with IFNy
[STEPS]
SECTION: Prior to sac
1. 3 days prior to sac
30minutes to an hour prior to Thioglycolate injection, giv... | ["[Prior to sac] 3 days prior to sac\n30minutes to an hour prior to Thioglycolate injection, give slow-release buprenorphine SubQ as a proactive measure against any pain or discomfort\nIntraperitoneal inject (IP) mice with 1mL of sterilized, 3% thioglycolate solution per mouse and return to home cage for 3 days.", "[Pr... |
68,563 | Coating superfrost microscope slides with gelatin-chromium potassium sulfate | 1 | dx.doi.org/10.17504/protocols.io.n2bvj65owlk5/v1 | https://www.protocols.io/view/coating-superfrost-microscope-slides-with-gelatin-ce7tthnn | Benjamin Trist, Alejandra Rangel, Rain Kwan | TITLE: Coating superfrost microscope slides with gelatin-chromium potassium sulfate
AUTHORS: Benjamin Trist, Alejandra Rangel, Rain Kwan
[DESCRIPTION]
This protocol describes how to coat microscope slides with gelatin-chromium potassium sulfate (gelatin-chrom alum) in preparation for histology or immunohistochemical a... | ["[Experimental Outline] Place heated magnetic stirrer in fume hood.", "[Experimental Outline] Pre-heat oven to 42 °C.", "[Experimental Outline] Heat 1 L dH20 to 50 °C - 60 °C and completely dissolve 10 g gelatin with aid of magnetic stirrer.", "[Experimental Outline] Add 1 g chromium potassium sulfate - solution shoul... |
36,865 | SOP for populating NCBI submission templates for SARS-CoV-2 (BioSample, SRA, and GenBank) | 1 | dx.doi.org/10.17504/protocols.io.bf89jrz6 | https://www.protocols.io/view/sop-for-populating-ncbi-submission-templates-for-s-bf89jrz6 | Ruth Timme, Emma Griffiths, Duncan MacCannell, Stacia Wyman | TITLE: SOP for populating NCBI submission templates for SARS-CoV-2 (BioSample, SRA, and GenBank)
AUTHORS: Ruth Timme, Emma Griffiths, Duncan MacCannell, Stacia Wyman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">PURPOSE: </span><span>Guidance on how to populate... | ["[Three templates needed for NCBI SARS-CoV-2 submission]\nGuidance for populating the three NCBI metadata templates for SARS-CoV-2 submission. START HERE FIRST: Read the PHA4GE contextual data specification BEFORE populating your submission templates! Steps 3-4 help to map the NCBI submission template fields to the... |
36,528 | Starting a MinION sequencing run using MinKNOW | null | dx.doi.org/10.17504/protocols.io.bfwqjpdw | https://www.protocols.io/view/starting-a-minion-sequencing-run-using-minknow-bfwqjpdw | Muhammad Faisal, Olin Silander, Nikki Freed | TITLE: Starting a MinION sequencing run using MinKNOW
AUTHORS: Muhammad Faisal, Olin Silander, Nikki Freed
[STEPS]
?. If required plug the MinION into the computer and wait for the MinION and flowcell to ben detected.
?. Choose flow cell 'FLO-MIN106' from the drop-down menu.
?. Then select the flowcell so a tick appea... | ["If required plug the MinION into the computer and wait for the MinION and flowcell to ben detected.", "Choose flow cell 'FLO-MIN106' from the drop-down menu.", "Then select the flowcell so a tick appears.", "Click the 'New Experiment' button in the bottom left of the screen.", "On the New experiment popup screen, sel... |
43,353 | mr test 19.10 | 1 | null | https://www.protocols.io/view/mr-test-19-10-bnjzmcp6 | Maria Guliakina | TITLE: mr test 19.10
AUTHORS: Maria Guliakina
[STEPS]
?. test 1
?. test 2 | ["test 1", "test 2"] |
107,164 | ILT3 | 0 | null | https://www.protocols.io/view/ilt3-dkv44w8w | Mar Roca Cugat, UM ILT Team | TITLE: ILT3
AUTHORS: Mar Roca Cugat, UM ILT Team
[DESCRIPTION]
-
[STEPS]
SECTION: Bacterial cell destruction
1. Resuspend the bacterial pellet in 0.3 mL of Resuspension Buffer.
SECTION: Bacterial cell destruction
1.1. Mix vigorously using the vortex until no clumps remain.
The solution needs to be “clear” not “turbi... | ["[Bacterial cell destruction] Resuspend the bacterial pellet in 0.3 mL of Resuspension Buffer.", "[Bacterial cell destruction] Mix vigorously using the vortex until no clumps remain. \nThe solution needs to be “clear” not “turbid” otherwise invert the tube a few times more and continue.\nThe lysate should appear visco... |
88,977 | Oligo Pool Resuspension and PCR | 4 | dx.doi.org/10.17504/protocols.io.8epv5xe3ng1b/v1 | https://www.protocols.io/view/oligo-pool-resuspension-and-pcr-c25ryg56 | NUS iGEM | TITLE: Oligo Pool Resuspension and PCR
AUTHORS: NUS iGEM
[DESCRIPTION]
2023 NUS-Singapore iGEM team followed this protocol to resuspend the dried oligo pool and perform PCR to amplify the DNA fragments in the oligo pool.
[STEPS]
SECTION: Oligo Pool Resuspension
1. Centrifuge the oligo pool for a few seconds before o... | ["[Oligo Pool Resuspension] Centrifuge the oligo pool for a few seconds before opening the tube.", "[Oligo Pool Resuspension] Calculate the volume of required for the oligo pool (the final concentration of the sample must be at least 20ng/μL).", "[Oligo Pool Resuspension] Add the respective volume of into the tube ... |
null | null | null | dx.doi.org/10.17504/protocols.io.iszcef6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
109,345 | Effect of different cadences on a cycle ergometer on blood pressure in people with hypertension: an empty systematic review of randomized controlled trials | 0 | dx.doi.org/10.17504/protocols.io.ewov19p6ylr2/v1 | https://www.protocols.io/view/effect-of-different-cadences-on-a-cycle-ergometer-dnz95f96 | Alexey Antonov, Alexander Miroshnikov, Polina Rybakova | TITLE: Effect of different cadences on a cycle ergometer on blood pressure in people with hypertension: an empty systematic review of randomized controlled trials
AUTHORS: Alexey Antonov, Alexander Miroshnikov, Polina Rybakova
[DESCRIPTION]
Current guidelines recommend exercise as part of primary and secondary prevent... | [] |
43,588 | Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs | 2 | dx.doi.org/10.17504/protocols.io.bntcmeiw | https://www.protocols.io/view/protocols-for-northern-analysis-of-exosome-substra-bntcmeiw | Jonathan Houseley, Cristina Cruz | TITLE: Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs
AUTHORS: Jonathan Houseley, Cristina Cruz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Over the past decade a plethora of noncoding RNAs (ncRNAs) have been identified, initiating an explosion in RNA research. Al... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.f26bqhe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><em>Sampling is for qPCR assay to determine total extracellular phage (qEXT), and for MPN (most probable number) assay to determine infective phage concentration</em>. <em>Samples containing phage and cells are filtered through a 0.2µm membrane in 96-well format to remove cel... | [] |
88,416 | Preparation of Agarose Gel | 4 | dx.doi.org/10.17504/protocols.io.5qpvo3o7xv4o/v1 | https://www.protocols.io/view/preparation-of-agarose-gel-c2j8ycrw | NUS iGEM | TITLE: Preparation of Agarose Gel
AUTHORS: NUS iGEM
[DESCRIPTION]
2023 NUS-Singapore iGEM team followed this protocol to prepare a 1% agarose gel. The team used this 1% agarose gel to isolate the DNA fragments from the common PCR and the colony PCR products.
[STEPS]
1. For a 1% agarose gel, mix the following in a co... | ["For a 1% agarose gel, mix the following in a conical flask: \n5 g of Agarose powder. \n50 mL of 1x TAE buffer (Tris-Acetate-EDTA Buffer).", "Swirl the agarose solution to mix it well.", "Heat the agarose solution in a microwave until it boils.", "Take out the conical flask from the microwave and swirl the conical fl... |
48,654 | FL-BEEHIV HIV-1 Genotyping and Drug Resistance by Next Generation Sequencing | 4 | dx.doi.org/10.17504/protocols.io.btrnnm5e | https://www.protocols.io/view/fl-beehiv-hiv-1-genotyping-and-drug-resistance-by-btrnnm5e | Brenna McGruder Rawson, Jason Blanton | TITLE: FL-BEEHIV HIV-1 Genotyping and Drug Resistance by Next Generation Sequencing
AUTHORS: Brenna McGruder Rawson, Jason Blanton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The Florida Department of Health's Bureau of Public Health Laboratories in Jacksonville is developing a protocol fo... | ["[RNA Extraction]\nExtract RNA using the Qiagen Viral RNA Mini Kit (DSP or RUO)", "[RNA Extraction]\nAdd per sample\n[carrier RNA]", "[RNA Extraction]\nFollow Kit instructions for extraction", "[cDNA Synthesis]\nMaster Mix\n[SuperScript IV VILO Master Mix]\n[Nuclease Free Water]\n[RNA template]", "[cDNA Synthesis]\n... |
55,096 | arc-melting | 1 | null | https://www.protocols.io/view/arc-melting-bz2yp8fw | Sterling G Baird | TITLE: arc-melting
AUTHORS: Sterling G Baird
[DESCRIPTION]
Perform arc melting of various substances.
[BEFORE_START]
Measure and record the mass of each of the starting materials prior to arc melting.
[GUIDELINES]
The goal is to obtain a homogenous compound consisting of several constituent materials. The minimum to... | ["[Setup]", "[Setup] The argon gas tank should be set to (right dial).", "[Setup]", "[Setup]", "[Setup] Turn on the power supply.", "[Arc Melting] Flip the power switch on for 5 s and then flip the power switch off.\n\n\n \nIf the materials haven't fully melted, run the arc melter for an additional 5 s while moving ... |
80,896 | Inhibitor-free DNA extraction from soil and sediment samples | 1 | dx.doi.org/10.17504/protocols.io.bp2l6957zlqe/v1 | https://www.protocols.io/view/inhibitor-free-dna-extraction-from-soil-and-sedime-cs88whzw | Dominik Buchner | TITLE: Inhibitor-free DNA extraction from soil and sediment samples
AUTHORS: Dominik Buchner
[DESCRIPTION]
This protocol describes how to extract inhibitor-free DNA from soil and sediment samples. 5 g of soil or up to 10 g of sediment can be processed in one extraction, but there is also a miniaturized version for 250... | ["[Protocol for up to 10 g of input material] Prepare one 50 mL centrifuge tube per sample with 15 g of garnet beads.", "[Protocol for up to 10 g of input material] Add up to 10 g of soil to the tube.", "[Protocol for up to 10 g of input material] Add 15 mL and 1.2 mL. Vortex shortly.", "[Protocol for up to 10 g of in... |
54,296 | SNP Genotyping and ApoE Genotyping | 1 | dx.doi.org/10.17504/protocols.io.by9ypz7w | https://www.protocols.io/view/snp-genotyping-and-apoe-genotyping-by9ypz7w | Huw Morris, Manuela MX Tan, Donald G Grosset, Nigel M Williams | TITLE: SNP Genotyping and ApoE Genotyping
AUTHORS: Huw Morris, Manuela MX Tan, Donald G Grosset, Nigel M Williams
[DESCRIPTION]
This protocol details the steps for DNA extraction from a human blood sample, quality control, and SNP and APOE genotyping.
The protocol has been adapted from the PRoBaND SNP Genotyping a... | ["[Quality Control and Principle Component Analysis] Perform standard quality control procedures in PLINK v1.9: https://www.cog-genomics.org/plink/", "[Quality Control and Principle Component Analysis] Remove:", "[Sample Collection and Genotyping] At study entry (baseline), collect a 4 mL blood sample in an ethylenedia... |
9,482 | Sequential smFISH Allen Institute | 1 | dx.doi.org/10.17504/protocols.io.mhic34e | https://www.protocols.io/view/sequential-smfish-allen-institute-mhic34e | Jennie Close, Zoe Maltzer | TITLE: Sequential smFISH Allen Institute
AUTHORS: Jennie Close, Zoe Maltzer
[DESCRIPTION]
We have developed a multiplexed single molecule FISH protocol for use at the Institute. This protocol was optimized on human tissue, but will work on mouse tissue as well. It was adapted from Lyubimova et. al., Nature Protocols, ... | ["[Tissue and Sectioning] 10-14 um cryosections are taken from fresh-frozen tissue, which are collected on poly-lysine-treated #1 coverslips at room temperature (RT). After 5-10 min at RT, sections are placed at 4°C until sectioning is complete. At that point, proceed immediately to fixation and permeabilization.", "[F... |
26,526 | Breeding NONcNZO10/LtJ (NON/NZ) and NON/ShiLtJ (NON/Shi) mice | null | dx.doi.org/10.17504/protocols.io.556g89e | null | Ian Simpson | TITLE: Breeding NONcNZO10/LtJ (NON/NZ) and NON/ShiLtJ (NON/Shi) mice
AUTHORS: Ian Simpson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block"><span style = "font-weight:bold;">♦</span><span style = "font-weight:bold;"> NON... | ["[Breeding]\n1. Mice are bred at 6 to 7 weeks of age or later. Set up two females with one male. 2. Every 6 months new male and females breeders are purchased from JAX laboratories. 3. The mice are set up for 5 days. Typically the mice are set up on a Thursday and the male is separated on the Tuesday and the females r... |
50,011 | private comments test | 1 | null | https://www.protocols.io/view/private-comments-test-bu33nyqn | Mariia Bc. Bc. Guliakina II | TITLE: private comments test
AUTHORS: Mariia Bc. Bc. Guliakina II
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">test</div></div>
[STEPS]
?. test 1 | ["test 1"] |
78,848 | Total RNA and DNA from Microalgae (12 samples per microplate) | 1 | dx.doi.org/10.17504/protocols.io.6qpvro85bvmk/v12 | https://www.protocols.io/view/total-rna-and-dna-from-microalgae-12-samples-per-m-cq88vzzw | Ying-Yu Hu | TITLE: Total RNA and DNA from Microalgae (12 samples per microplate)
AUTHORS: Ying-Yu Hu
[DESCRIPTION]
Presented herein is a protocol for the extraction and quantification of bulk RNA and DNA from microalgae, adapted from the methodology outlined by Berdalet et al. (2005). RNA and DNA are extracted from microalgae sa... | ["[Sample collection] Freeze-dry samples. Store at -80 °C.", "[Primary solutions] Turn on UV light in biosafety cabinet for 15 min", "[Primary solutions] Clean working surface with decontamination solution.", "[Primary solutions] Prepare Tris buffer 5 mM pH 8.0", "[Primary solutions] Pour 1 M pH 8.0 Tris into an RNase... |
null | null | null | dx.doi.org/10.17504/protocols.io.gwpbxdn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
32,370 | Proteomic protocol for Tibetan hulless barley under osmotic stress | null | dx.doi.org/10.17504/protocols.io.bbusinwe | null | Yulin Wang, Zha Sang, Shaohang Xu, Qijun Xu, Xingquan Zeng, Dunzhu Jabu, Hongjun Yuan | TITLE: Proteomic protocol for Tibetan hulless barley under osmotic stress
AUTHORS: Yulin Wang, Zha Sang, Shaohang Xu, Qijun Xu, Xingquan Zeng, Dunzhu Jabu, Hongjun Yuan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the complete procedures for performing data‐independent acq... | ["[Protein extraction]\nWeigh 1-g subsample and homogenized by grinding in liquid nitrogen, moved the powdered samples to 50 cm3 tubes;", "[Protein extraction]\nAdd 25 cm3 precooled acetone (-20 °C) containing 10% (v/v) trichloroacetic acid (TCA) and 10 mM dithiothreitol (DTT);\n[precooled acetone (-20 °C)]", "[Protein... |
null | null | null | dx.doi.org/10.17504/protocols.io.tc3eiyn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>CAR T is a T cell modified by genetic recombination technology. CAR in CAR-T is a recombinant receptor, and its structure mainly includes three parts: extracellular antigen binding domain, transmembrane domain and intracellular signal domain.</p>
[STEPS]
?. | [] |
86,491 | Squishing insects for preservation of HMW DNA in the field | 4 | dx.doi.org/10.17504/protocols.io.4r3l2224jl1y/v1 | https://www.protocols.io/view/squishing-insects-for-preservation-of-hmw-dna-in-t-cyp3xvqn | Fiona Teltscher, Mara Lawniczak | TITLE: Squishing insects for preservation of HMW DNA in the field
AUTHORS: Fiona Teltscher, Mara Lawniczak
[DESCRIPTION]
Ideal preservation for maximum recovery of high molecular weight (HMW) DNA from insects is to snap freeze living insects on dry ice and store at -70C. However, dry ice or liquid nitrogen for snap ... | ["Transfer your live caught mosquito into to kill it.", "With clean forceps remove the mosquito from and gently dab it on a clean wipe (e.g. Kimtech wipe) to dry off any remaining ethanol. Transfer it into a 0.5 mL Eppendorf tube (or similar) filled with 400 µL of preservation liquid of your choice. \n\n[If you ar... |
null | null | null | dx.doi.org/10.17504/protocols.io.mpbc5in | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?. | [] |
82,332 | Mouse synapse imaging and analysis | 3 | dx.doi.org/10.17504/protocols.io.kxygx9jjog8j/v1 | https://www.protocols.io/view/mouse-synapse-imaging-and-analysis-cum4wu8w | Shiyi Wang | TITLE: Mouse synapse imaging and analysis
AUTHORS: Shiyi Wang
[DESCRIPTION]
Mouse synapse imaging and analysis
[STEPS] | [] |
12,446 | ARDRA: amplified rDNA restriction analysis | 1 | dx.doi.org/10.17504/protocols.io.x54v96zzv3eq/v1 | https://www.protocols.io/view/ardra-amplified-rdna-restriction-analysis-qd6ds9e | Eva Petrova, Roey Angel | TITLE: ARDRA: amplified rDNA restriction analysis
AUTHORS: Eva Petrova, Roey Angel
[DESCRIPTION]
ARDRA: amplified rDNA restriction analysis
1. Sklarz MY, Angel R, Gillor O, Soares MIM. Amplified rDNA Restriction Analysis (ARDRA) for Identification and Phylogenetic Placement of 16S-rDNA Clones. In: de Bruijn FJ, edi... | ["[PCR] A direct colony PCR is best used for this purpose, reaction mix might vary according to the specific Taq polymerase used. This following mix yields a strong amplification so that only 12 µL reaction is required. \nThe following mix was used with Hy Labs' Taq polymerase. If different Taq is used, re-optimization... |
97,918 | Green Lab Nanoparticle Prep For In Vivo | 0 | null | https://www.protocols.io/view/green-lab-nanoparticle-prep-for-in-vivo-dbu62nze | Edwin Yoo | TITLE: Green Lab Nanoparticle Prep For In Vivo
AUTHORS: Edwin Yoo
[DESCRIPTION]
draft
[STEPS]
SECTION: Background (Materials in bold)
1. This protocol is to generate 3x100ul DNA nanoparticle injections at 40 w/w (DNA:polymer) and 0.25 mg/ml DNA.
-100 ul injections per mouse, 350 ul NP+DNA per frozen tube (thaw before... | ["[Background (Materials in bold)] This protocol is to generate 3x100ul DNA nanoparticle injections at 40 w/w (DNA:polymer) and 0.25 mg/ml DNA.\n-100 ul injections per mouse, 350 ul NP+DNA per frozen tube (thaw before animal injection).\n-DNA stock should be 1 ug/ul\n-Polymer stock should be 100 ug/ul\n-For 40 w/w, 88 ... |
81,431 | Reto F1016B | 1 | dx.doi.org/10.17504/protocols.io.q26g7y2mqgwz/v1 | https://www.protocols.io/view/reto-f1016b-ctrxwm7n | Camila Miranda Bajo Gutiérrez, Diana Paulina Arroyo Soltero | TITLE: Reto F1016B
AUTHORS: Camila Miranda Bajo Gutiérrez, Diana Paulina Arroyo Soltero
[DESCRIPTION]
Pasos de como realizar el circuito que se necesita para el reto F1016B
[STEPS]
SECTION: Preparación del circuito
1. Reúne todos los materiales que se necesitan y coloca la protoboard en tu área de trabajo.
SECTION: P... | ["[Preparación del circuito] Reúne todos los materiales que se necesitan y coloca la protoboard en tu área de trabajo.", "[Preparación del circuito] Conecte la sonda del osciloscopio y conectela a la protoboard.", "[Ejecución del experimento.] Encienda la fuente de carga y calibre el voltaje a 5 Volts.", "[Ejecución de... |
50,535 | What to include in your protocol | 1 | dx.doi.org/10.17504/protocols.io.bvkfn4tn | https://www.protocols.io/view/what-to-include-in-your-protocol-bvkfn4tn | Emma Ganley, Anita Broellochs, Lenny Teytelman | TITLE: What to include in your protocol
AUTHORS: Emma Ganley, Anita Broellochs, Lenny Teytelman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A list to advise on the different elements that are possible to include as part of your protocol in protocols.io. If possible, please include these in the p... | ["Full Author ListIf the protocol should have a specific author list, please be sure to include all author names and affiliations comprehensively in the order that you would like them to appear.", "AbstractIf you intend to publish your protocol, please include a short abstract to give an insight into the protocol, its ... |
25,849 | Flow Cytometry-Based Gamma-H2AX Assay for Radiation Biodosimetry | null | dx.doi.org/10.17504/protocols.io.5gzg3x6 | null | Ghazi Alsbeih, Krishna Mishra, Maha Alrashd, Subramanian M. Pulicat, Najla Al-Harbi, Sara Bin Judia, Belal Moftah | TITLE: Flow Cytometry-Based Gamma-H2AX Assay for Radiation Biodosimetry
AUTHORS: Ghazi Alsbeih, Krishna Mishra, Maha Alrashd, Subramanian M. Pulicat, Najla Al-Harbi, Sara Bin Judia, Belal Moftah
[STEPS]
?. [A. Lymphocytes separation and fixation:]
Collect blood samples in heparinized Vacutainers (BD, USA).
?. [A. Lymp... | ["[A. Lymphocytes separation and fixation:]\nCollect blood samples in heparinized Vacutainers (BD, USA).", "[A. Lymphocytes separation and fixation:]\nIrradiate 2 ml aliquot of whole blood with the required radiation dose.", "[A. Lymphocytes separation and fixation:]\nIncubate for 30 minutes (or other time) at 37ºC.", ... |
68,134 | Caracterización Ambiental de la Zona Estuarina del Golfo de Montijo, Provincia de Veraguas, Panamá | 1 | dx.doi.org/10.17504/protocols.io.6qpvr6b3bvmk/v1 | https://www.protocols.io/view/caracterizaci-n-ambiental-de-la-zona-estuarina-del-cesetebe | ana.garcia., Diana Araúz, Eridna Martínez, Jay Molino | TITLE: Caracterización Ambiental de la Zona Estuarina del Golfo de Montijo, Provincia de Veraguas, Panamá
AUTHORS: ana.garcia., Diana Araúz, Eridna Martínez, Jay Molino
[DESCRIPTION]
El protocolo aquí descrtio se basa en los trabajos realizados en el estudio Caracterización ambiental de la zona estuarina del Golfo de... | ["[Protocolo de Colecta de Muestras de Sedimento Marino] Colecta de muestras de sedimentos marinos\nPara la colecta de las muestras de sedimento marino se uso el protocolo de la Guía Nacional de Colecta y Preservación de Muestras: aguas, sedimentos, comunidades acuáticas y efluentes líquidos, Compañía Ambiental del Est... |
86,094 | Measuring mitophagy via FACS with mtKeima reporter | 1 | null | https://www.protocols.io/view/measuring-mitophagy-via-facs-with-mtkeima-reporter-cybnxsme | Louise Uoselis | TITLE: Measuring mitophagy via FACS with mtKeima reporter
AUTHORS: Louise Uoselis
[DESCRIPTION]
Preparation of samples for measuring mitophagy levels using mtKeima reporter by FACS
[STEPS]
SECTION: Day 1
1. Seed cells in a 24 well plate, aiming for a confluency of ~80-90% at the time of treatment. Seed additional wel... | ["[Day 1] Seed cells in a 24 well plate, aiming for a confluency of ~80-90% at the time of treatment. Seed additional wells of cells not expressing any fluorescent proteins, cells expressing only mtKeima, and cells expressing only YFP-Parkin (to be used as gating controls).", "[Day 2] Feed all cells with standard growt... |
33,464 | RNA isolation and RT-qPCR for Dengue, Chikungunya and Zika Viruses | 1 | dx.doi.org/10.17504/protocols.io.bcwyixfw | https://www.protocols.click/view/rna-isolation-and-rt-qpcr-for-dengue-chikungunya-a-bcwyixfw | Maria Fernanda Avila Mejia, Pei-Yun Shu, Dar-Der Ji | TITLE: RNA isolation and RT-qPCR for Dengue, Chikungunya and Zika Viruses
AUTHORS: Maria Fernanda Avila Mejia, Pei-Yun Shu, Dar-Der Ji
[DESCRIPTION]
This protocol is for dried blood spot (DBS) sample collection, the extraction of viral RNA from dried blood spots, and the following RT-qPCR setup for the detection of De... | ["[RNA Viral Extraction] 1. Use the paper puncher to punch a 6-mm punch in the dried blood spot. Sterilize the paper puncher with fire and make a clean punch after each use to avoid cross-contamination.\n\n2. Extract Viral RNA utilizing the QIAamp Viral RNA Mini Kit according to the manufacturer's instructions..", "[R... |
46,006 | Slice Preparation with Tissue Dissociation - Mouse Protocol | 1 | dx.doi.org/10.17504/protocols.io.bq6wmzfe | https://www.protocols.io/view/slice-preparation-with-tissue-dissociation-mouse-p-bq6wmzfe | Allen Institute for Brain Science | TITLE: Slice Preparation with Tissue Dissociation - Mouse Protocol
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the procedure for transcardial perfusion of an adult mouse, followed by brain dissection, embedding, slicing, blockfac... | [] |
59,208 | Unclear insomnia types in randomized controlled trials and systematic reviews: the protocol for a meta-epidemiological study | 1 | dx.doi.org/10.17504/protocols.io.b53gq8jw | https://www.protocols.io/view/unclear-insomnia-types-in-randomized-controlled-tr-b53gq8jw | Masahiro Banno, Yasushi Tsujimoto, Kunihiro Kohmura, Eisuke Dohi, Shunsuke Taito, Hidehiro Someko, Yuki Kataoka | TITLE: Unclear insomnia types in randomized controlled trials and systematic reviews: the protocol for a meta-epidemiological study
AUTHORS: Masahiro Banno, Yasushi Tsujimoto, Kunihiro Kohmura, Eisuke Dohi, Shunsuke Taito, Hidehiro Someko, Yuki Kataoka
[DESCRIPTION]
Objectives. To examine tendencies and charac... | ["[BACKGROUND] Insomnia disorder is diagnosed when both nocturnal insomnia symptoms and daytime dysfunctions continued for at least 1 month in the tenth revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-10), and for at least 3 months in the third edition of the Intern... |
87,560 | Gewebesammlung Frischgewebe Zystektomie | 1 | null | https://www.protocols.io/view/gewebesammlung-frischgewebe-zystektomie-czrgx53w | Annika Fendler, Bettina Ergün | TITLE: Gewebesammlung Frischgewebe Zystektomie
AUTHORS: Annika Fendler, Bettina Ergün
[DESCRIPTION]
Dieses Protokoll beschreibt die Schritte für die Sammlung von Frischgewebe, Gefriergewebe (Fresh-frozen), und Blut von Patienten mit Harnblasenkarzinom nach Zystektomie.
Verwandte Dokumente:
Protokoll zur Blutaufarb... | ["[Dokumentation] Patientendaten in Datei hinterlegen und korrespondierende Liquidnummer eintragen: \\\\Charite.de\\Centren\\C08\\UR\\FO\\Intern\\PROBEN_PATIENTEN\\01-Gewebe\\Gewebe_FF\\ Biobank_Gewebe_ab01012019.xlsx", "[Dokumentation] In der Liquid-Sammelliste die korresspondierende Gewebenummer eintragen: \\\\Charit... |
100,129 | Multi-electrode array recordings | 0 | dx.doi.org/10.17504/protocols.io.8epv5rj86g1b/v1 | https://www.protocols.io/view/multi-electrode-array-recordings-ddz92796 | Nicola Biagio Mercuri | TITLE: Multi-electrode array recordings
AUTHORS: Nicola Biagio Mercuri
[DESCRIPTION]
MEA recordings in acute slices
[STEPS]
SECTION: Spike detection
1. Individual slices are placed over an 8 × 8 array of planar microelectrodes, each 20μm×20μm in size,
with an interpolar distance of 100 μm (MED-P2105; Alpha MED Scienc... | ["[Spike detection] Individual slices are placed over an 8 × 8 array of planar microelectrodes, each 20μm×20μm in size,\nwith an interpolar distance of 100 μm (MED-P2105; Alpha MED Sciences, Kadoma, Japan). Slices are positioned over the multi-electrode array under visual control through an upright microscope (Leica DM... |
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