id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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58,072 | Preparation and use of 12-well plates for the rapid detection of terbinafine-resistant dermatophytes | 4 | dx.doi.org/10.17504/protocols.io.bp2l6116zvqe/v1 | https://www.protocols.io/view/preparation-and-use-of-12-well-plates-for-the-rapi-b4xyqxpw | Khalid El Moussaoui | TITLE: Preparation and use of 12-well plates for the rapid detection of terbinafine-resistant dermatophytes
AUTHORS: Khalid El Moussaoui
[DESCRIPTION]
This protocol describes the steps necessary for the preparation and use of screening plates for the rapid detection of terbinafine resistant dermatophytes.
[GUIDELIN... | ["[Medium preparation] Dissolve 30 g of in 1 L of and let mix on the heated magnetic stirrer for 5 min (temperature and mixing speed knob at mid-step)", "[Medium preparation] Add 15 g of and let dissolve for 10 min while mixing and heating", "[Medium preparation] Pour 250 mL of this medium into 4 erlenmeyer flas... |
66,316 | Immunity 911: Protect Against Toxins and Infections! | 1 | dx.doi.org/10.17504/protocols.io.5qpvob6ybl4o/v1 | https://www.protocols.io/view/immunity-911-protect-against-toxins-and-infections-cczksx4w | hkdfjzae | TITLE: Immunity 911: Protect Against Toxins and Infections!
AUTHORS: hkdfjzae
[DESCRIPTION]
Product Name - Immunity 911
Purpose - Immune Booster
Administration Route - Oral
Dosage - Take 2 Capsules a day
Unit Count - 60 Capsules
Side Effects - No major side effects reported
Price - $69.95
Availability - Only thro... | [] |
39,278 | Methanol-Chloroform-Water Precipitation | 1 | dx.doi.org/10.17504/protocols.io.biknkcve | https://www.protocols.io/view/methanol-chloroform-water-precipitation-biknkcve | Bryon Drown, Kelleher Research Group | TITLE: Methanol-Chloroform-Water Precipitation
AUTHORS: Bryon Drown, Kelleher Research Group
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Methanol-chloroform-water (MCW) precipitation is a rapid method for removing interferring species from protein samples prior to LC-MS analysis. MCW precipitati... | ["Dilute protein sample to 150 µL with Optima-grade water.", "Precipitate protein at interface", "Pellet protein at interface", "Wash pellet with methanol. (This step can be repeated if a high level of contaminating species is anticipated)", "Redissolve protein in LC buffer", "Add 600 µL Optima-grade methanol and mix w... |
31,090 | Ancient DNA Extraction from Skeletal Material | 1 | dx.doi.org/10.17504/protocols.io.baksicwe | https://www.protocols.io/view/ancient-dna-extraction-from-skeletal-material-baksicwe | Irina Velsko, Eirini Skourtanioti, Guido Brandt | TITLE: Ancient DNA Extraction from Skeletal Material
AUTHORS: Irina Velsko, Eirini Skourtanioti, Guido Brandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Silica-based total DNA extraction protocol optimised for the recovery of ultra-short DNA molecules from skeletal material powder (e.g. b... | ["[Day 1: Binding buffer preparation (Buffer Prep Room)]\nPrepare binding buffer calculating . ABC1Reagent [Stock Concentration]Final ConcentrationVolume/reaction2GuHCl (1 mol=95.53 g)5 M4.77 g3UV HPLC-water up to6 ml4Isopropanol (100%)40%4 ml5Total10 ml\n[/ reaction]\nABC1Reagent [Stock Concentration]Final Concentrat... |
null | null | null | dx.doi.org/10.17504/protocols.io.j3bcqin | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes a procedure how primary metabolites and proteins can be extracted from <em>Deinococcus radiodurans</em> simultaneously. Polar metabolites are purified and derivatized for GC-MS (TOF) analysis. Proteins are tryptically digested in-gel and peptides are p... | [] |
64,290 | ONT DNA Barcoding Fungal Amplicons w/ MinION & Flongle | 2 | dx.doi.org/10.17504/protocols.io.36wgq7qykvk5/v1 | https://www.protocols.io/view/ont-dna-barcoding-fungal-amplicons-w-minion-amp-fl-ca2asgae | Stephen Douglas Russell | TITLE: ONT DNA Barcoding Fungal Amplicons w/ MinION & Flongle
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
This collection of protocols outline a working methodology to DNA barcode fungal specimens. This process will work for dried tissue, fresh tissue, or with DNA template that has already gone through a... | [] |
58,447 | Chloramphenicol 25 mg/mL Stock Solution | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn7ewvx9/v1 | https://www.protocols.io/view/chloramphenicol-25-mg-ml-stock-solution-b5bpq2mn | Bailey Clark | TITLE: Chloramphenicol 25 mg/mL Stock Solution
AUTHORS: Bailey Clark
[DESCRIPTION]
The abstract will be added later.
[STEPS]
SECTION: Preparing the Stock Solution
1. Weigh 25 mg (0.025 g ) of Chloramphenicol.
SECTION: Preparing the Stock Solution
2. Add the chloramphenicol to a 1.5 mL tube.
SECTION: Preparing the ... | ["[Preparing the Stock Solution] Weigh 25 mg (0.025 g ) of Chloramphenicol.", "[Preparing the Stock Solution] Add the chloramphenicol to a 1.5 mL tube.", "[Preparing the Stock Solution] Add 100% ethanol to the 1mL level on the tube.", "[Preparing the Stock Solution] Vortex the tube for 5 seconds, repeat 3 times.", "[Pr... |
43,575 | RNA Electrophoresis in Agarose Gels | 4 | dx.doi.org/10.17504/protocols.io.bnsxmefn | https://www.protocols.io/view/rna-electrophoresis-in-agarose-gels-bnsxmefn | Jonathan Houseley, Cristina Cruz | TITLE: RNA Electrophoresis in Agarose Gels
AUTHORS: Jonathan Houseley, Cristina Cruz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Over the past decade a plethora of noncoding RNAs (ncRNAs) have been identified, initiating an explosion in RNA research. Although RNA sequencing methods provide unsur... | ["[RNA Electrophoresis in Agarose Gels]\nNote that the method described below is for a 15 × 15 cm gel.", "[RNA Electrophoresis in Agarose Gels]\nAdd ethidium bromide to an aliquot of glyoxal mix to a final concentration of .", "[RNA Electrophoresis in Agarose Gels]\nMix with (can use less of RNA sample), then incubat... |
26,446 | Measure 40 worm feeding with bioluminescent bacteria after drug treatment | null | dx.doi.org/10.17504/protocols.io.53ng8me | null | Serena Ding | TITLE: Measure 40 worm feeding with bioluminescent bacteria after drug treatment
AUTHORS: Serena Ding
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protocol uses bioluminescently labelled bacteria (Gregor et al, 2008, Addgene Plasmid #107879, </span><a href="https://www.pnas.org/content/115/... | ["[Grow bacteria overnight]\nDay -2 Streak out bioluminescent bacteria frozen stock onto an LB plate containing 50 μg/mL ampicillin and incubate overnight at .\n37 °C\nThis step does not need to be performed every time. A streaked plate can be re-used within 1 month, so long as single colonies are still available. Othe... |
28,453 | Endometrium dissociation with trypsin | null | dx.doi.org/10.17504/protocols.io.72dhqa6 | null | Regina Hoo, Roser Vento-Tormo | TITLE: Endometrium dissociation with trypsin
AUTHORS: Regina Hoo, Roser Vento-Tormo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for enrichment of epithelial glands on endometrium; following the step from "Endometrium dissociation with collagenase".</div></div>
[STEPS]
?. Follo... | ["Following step 10 from \"Endometrium dissociation with collagenase\" protocol, where pieces of tissue are retained on the cell strainer. Collect pieces retained on the filter by inverting the filter into a new 50 mL tub and adding 45 ml of PBS with a 1 mL pipette.", "Centrifuge at 450 g for together with collagenase... |
88,739 | Preparation of Tissue Samples for DNA Extraction and Copy Number Analysis by iDNA Technologies | 1 | null | https://www.protocols.io/view/preparation-of-tissue-samples-for-dna-extraction-a-c2wbyfan | Lynn Doran | TITLE: Preparation of Tissue Samples for DNA Extraction and Copy Number Analysis by iDNA Technologies
AUTHORS: Lynn Doran
[DESCRIPTION]
Preparation of leaf tissue for shipment to iDNA Genetics for DNA extraction and copy number analysis.
NOTE: TOBACCO TISSUE IS REGULATED AS DRUGS AND ALCOHOL. IT CANNOT BE SHIPPED... | ["[Get a Quote] Email iDNA Technologies for a quote. Include the number of samples to be tested, which genes you want copy number analysis on, and which species the samples are from. \n\nInclude one sample with a known copy number of 2 as an internal reference standard for each assay (gene).", "[Get a Quote] When you'... |
30,473 | Immunoprecipitation (IP) and Mass Spectrometry | 1 | null | https://www.protocols.io/view/immunoprecipitation-ip-and-mass-spectrometry-9zhh736 | Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying | TITLE: Immunoprecipitation (IP) and Mass Spectrometry
AUTHORS: Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol explains Imm... | ["[Immunoprecipitation (IP)]\nTwenty-four hours after mRNA transfection, extract nuclear proteins of iPSCs using the NE-PER Nuclear and Cytoplasmic Extraction Kit.", "[Immunoprecipitation (IP)]\nPerform FLAG IP using anti-FLAG M2 antibody and Protein G Dynabeads following the manufacturer’s protocol.", "[Immunoprecipit... |
85,002 | Using a Sonicator Bath to Clean the Nozzle on a BD FACS Aria II/III/Fusion, SONY SY3200, or Other Instrumentation with Similarly Sized Nozzles | 1 | dx.doi.org/10.17504/protocols.io.rm7vzx31rgx1/v1 | https://www.protocols.click/view/using-a-sonicator-bath-to-clean-the-nozzle-on-a-bd-cw9ixh4e | Jamie C Tijerina | TITLE: Using a Sonicator Bath to Clean the Nozzle on a BD FACS Aria II/III/Fusion, SONY SY3200, or Other Instrumentation with Similarly Sized Nozzles
AUTHORS: Jamie C Tijerina
[DESCRIPTION]
Sonicating the nozzle is an important step when dealing with clogs on a cell sorter. In sorters where the nozzle positioning is n... | ["Wipe down the interior of the sonicator bath with a CaviWipe or 70% ethanol", "Check that the sonicator is connected and the power is on.", "Very carefully fill the sonicator bath to operating line with DI water from MilliQ or similar dispenser.\nDo not use regular tap water.\nDo not fill a small receptacle in lieu o... |
34,620 | PCR Protocol for OneTaq® DNA Polymerase (M0480) | 1 | dx.doi.org/10.17504/protocols.io.bd24i8gw | https://www.protocols.io/view/pcr-protocol-for-onetaq-dna-polymerase-m0480-bd24i8gw | New England Biolabs | TITLE: PCR Protocol for OneTaq® DNA Polymerase (M0480)
AUTHORS: New England Biolabs
[DESCRIPTION]
The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification.Taq DNA Polymerase is an enzyme widely used in PCR. OneTaq DNA Polymerase allows for greater amplification sensitivity acr... | ["Set up the following reaction on ice:\n Component 25 μl reaction 50 μl reaction Final Concentration 5X OneTaq Standard", "Gently mix the reaction.", "Collect all liquid to the bottom of the tube by a quick spin if necessary and overlay the sample with mineral oil if using a PCR machine without a heated lid.", "Qu... |
69,175 | Olfactory mucosa sampling and processing | 1 | null | https://www.protocols.io/view/olfactory-mucosa-sampling-and-processing-cfsxtnfn | maria.xylaki | TITLE: Olfactory mucosa sampling and processing
AUTHORS: maria.xylaki
[DESCRIPTION]
This protocol describes the olfactory mucosa sampling and processing for RT-QuIC assays and was adapted from A. Stefani et al. (doi:10.1093/brain/awab005).
[STEPS]
SECTION: Olfactory mucosa sampling
1. Two olfactory mucosa samples ar... | ["[Olfactory mucosa sampling] Two olfactory mucosa samples are collected per person, one nasal swabbing from each nostril.\nA sterile, disposable swab is inserted in the nasal cavity, (similar to a covid test but more towards the top) to reach the olfactory and not the epithelial mucosa. \nThe swab is gently rolled on ... |
null | null | null | dx.doi.org/10.17504/protocols.io.grjbv4n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong style="box-sizing: border-box; margin: 0px; padding: 0px; border: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-numeric: inherit; font-weight: bold; font-stretch: inherit; font-size: 14px; line-height: inherit; font-f... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fktbkwn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Experiment purpose is to monitor the time-course of a large-scale infection of host cyanobacteria by phage under variable media conditions and obtain samples for proteomic and transcriptomic analysis.</p>
<p> </p>
<p>As a minimum, prepare 12 sample bottles for cells infected ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.e2ibgce | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p>-Protocol can be used for Beta Amyloid products that list “IHC” as an application on the datasheet (ex 4G8, 6E10, etc)</p>
<p> </p>
<p>-Use with Ultra Streptavidin Detection Kit (<a href="https://antibody.biolegend.com/datasheet.php?UpProd=&catalogno=SIG-32250" target="_bl... | [] |
81,944 | Complete Medium or Complete Medium Xylose (from Leach, Lang and Yoder 1982) | 4 | null | https://www.protocols.io/view/complete-medium-or-complete-medium-xylose-from-lea-ct9ywr7w | Megan Mcdonald | TITLE: Complete Medium or Complete Medium Xylose (from Leach, Lang and Yoder 1982)
AUTHORS: Megan Mcdonald
[DESCRIPTION]
For the growth and maintenance of Cochliobolus carbonum and Cochliobolus victoriae
[STEPS]
SECTION: Make 100x Salt Solutions A and B
2. 100X Salt Solution A
10g Ca(NaO3)2 * 4 H2O
100 mL ddH2O
Au... | ["[Make 100x Salt Solutions A and B] 100X Salt Solution A\n\n10g Ca(NaO3)2 * 4 H2O\n100 mL ddH2O\n\nAutoclave\n\nCitation:\nLeach, J., Lang, B. R. & Yoder, O. C. Microbiology128, 1719-1729, doi:https://doi.org/10.1099/00221287-128-8-1719 (1982).", "[Make 100x Salt Solutions A and B] 100X Salt Solution B\n\n2 g KH2PO4 ... |
null | null | null | dx.doi.org/10.17504/protocols.io.p4ndqve | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes the recipe for apple-agar plates which can be used for embryo collection. Alternatively, grape juice can be used instead of apple juice.</p>
[GUIDELINES]
<p>This protocol describes how to make sufficient apple-agar for approximatley 10x 90mm Petri dis... | [] |
44,083 | RNA to cDNA and RT-PCR protocol | 4 | dx.doi.org/10.17504/protocols.io.bpatmien | https://www.protocols.io/view/rna-to-cdna-and-rt-pcr-protocol-bpatmien | Heather Robeson, Jing Jin, Mohammed S. Orloff | TITLE: RNA to cDNA and RT-PCR protocol
AUTHORS: Heather Robeson, Jing Jin, Mohammed S. Orloff
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Used applied biosystems high-capacity RNA-to-cDNA kit and fast SYBR green master mix protocol</div></div>
[STEPS]
?. [RNA to cDNA reverse transcription]
Prep... | ["[RNA to cDNA reverse transcription]\nPrepare the RT reaction mix2X buffer mix :20X RT enzyme Mix: RNA sample: Nuclease-free water: quantity sufficient to Total per reaction:\n10 µl\n1 µl\n9 µl\n20 µl\n20 µl", "[RNA to cDNA reverse transcription]\nPrepare the reverse transcription reactions:Aliquot RT reaction mix i... |
49,398 | Mutational Analysis of Hepatitis B Virus Precore Region Molecular Variants in Ile-ife, Nigeria | 4 | dx.doi.org/10.17504/protocols.io.bugwntxe | https://www.protocols.io/view/mutational-analysis-of-hepatitis-b-virus-precore-r-bugwntxe | Oluwadamilola Osasona, Olumuyiwa Ariyo | TITLE: Mutational Analysis of Hepatitis B Virus Precore Region Molecular Variants in Ile-ife, Nigeria
AUTHORS: Oluwadamilola Osasona, Olumuyiwa Ariyo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><span style = "font-weight:bold;">Abstract</span><... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gmnbu5e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Use for solid phase culture and chemical selection of <em>Pseudo-nitzschia spp</em> and other recalcitrant diatoms</p>
[GUIDELINES]
<p>Reagents:</p>
<ul>
<li>0.2 μm filtered seawater (FSW)</li>
<li>L1 Medium Kit (ncma.bigelow.org)</li>
<li>SeaPrep™ Agarose (Lonza Cat#: 50302... | [] |
87,598 | DNA extraction (BOMB) | 4 | dx.doi.org/10.17504/protocols.io.n2bvj6mdnlk5/v8 | https://www.protocols.io/view/dna-extraction-bomb-czsnx6de | Yin-Tse Huang, Tsu-Chun Hung | TITLE: DNA extraction (BOMB)
AUTHORS: Yin-Tse Huang, Tsu-Chun Hung
[DESCRIPTION]
DNA extraction (BOMB)
[STEPS]
SECTION: Sample Collection
1. Add 200 µL of 0.5 mm beads to 2mL screw tube
SECTION: Sample Collection
2. Add200 µL of 1 mm beads to 2mL screw tube
SECTION: Sample Collection
3. Add870 µL Lysis master m... | ["[Sample Collection] Add 200 µL of 0.5 mm beads to 2mL screw tube", "[Sample Collection] Add200 µL of 1 mm beads to 2mL screw tube", "[Sample Collection] Add870 µL Lysis master mix to 2mL screw tube. The final look:", "[Sample Collection] Collect 20-50 mg of sample to 2mL screw tube", "[Sample crush] Put the 2mL screw... |
43,796 | Lab 3 Notebook (Draft) | 3 | null | https://www.protocols.io/view/lab-3-notebook-draft-bnzumf6w | TITLE: Lab 3 Notebook (Draft)
AUTHORS:
[STEPS] | [] | |
35,999 | NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428) | null | dx.doi.org/10.17504/protocols.io.bfd7ji9n | https://www.protocols.io/view/nebexpress-ni-resin-gravity-flow-typical-protocol-bfd7ji9n | New England Biolabs | TITLE: NEBExpress Ni Resin Gravity Flow Typical Protocol (NEB #S1428)
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for u... | ["[Guidelines for Ni Resin Cleaning]\nTo remove lipoproteins, lipids and proteins strongly bound by hydrophobic interaction follow one of these three methods:Add to the column and allow a contact time of to , followed by 10 CV of Lysis/Binding Buffer.Add 30% isopropanol to the column and allow a contact time of -, fo... |
106,910 | Annotation for Fungi | 0 | dx.doi.org/10.17504/protocols.io.e6nvw14nwlmk/v3 | https://www.protocols.io/view/annotation-for-fungi-dkm64u9e | Sebastian Bassi, Virginia Gonzalez, Tristan Yang | TITLE: Annotation for Fungi
AUTHORS: Sebastian Bassi, Virginia Gonzalez, Tristan Yang
[DESCRIPTION]
Protocol to annotate a fungi genome
[STEPS]
SECTION: Setup
1. Install Docker
If you don't have Docker already, install it. There are two versions, Docker Engine (also known as CE) and Docker Desktop. The Desktop versi... | ["[Setup] Install Docker\n\nIf you don't have Docker already, install it. There are two versions, Docker Engine (also known as CE) and Docker Desktop. The Desktop version is more user friendly but since may require commercial license for large enterprise, this tutorial is based on the Docker engine. Both version will w... |
19,679 | Binary synthetic template oligonucleotide positve control for in-house diagnostic real-time RT-PCR | null | dx.doi.org/10.17504/protocols.io.xf7fjrn | null | Ian Mackay, Judy Northill | TITLE: Binary synthetic template oligonucleotide positve control for in-house diagnostic real-time RT-PCR
AUTHORS: Ian Mackay, Judy Northill
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details how to make and use a pair of synthetic template oligonucleotides (STOs) for use as real-... | ["[Overview]\nThis protocol describes the design and use of a binary positive control system for use in clinical biospecimen testing. The end products avoid the need to handle infectious wild-type virus or rely on previously positive clinical specimens for a PCR positive control source. Ours is a public health virology... |
63,211 | Preparation of primary rat cortical neuron and astrocyte co-culture | 4 | dx.doi.org/10.17504/protocols.io.n92ldzq1xv5b/v1 | https://www.protocols.io/view/preparation-of-primary-rat-cortical-neuron-and-ast-b9yjr7un | mineechoi | TITLE: Preparation of primary rat cortical neuron and astrocyte co-culture
AUTHORS: mineechoi
[DESCRIPTION]
This protocol describes how to prepare primary rat cortical neuron and astrocyte co-culture.
[STEPS]
1. 1-3 days postpartum Sprague Dawley rats (University College London breeding colony) are used.
Experimen... | ["1-3 days postpartum Sprague Dawley rats (University College London breeding colony) are used. \nExperimental procedures are performed according to the United Kingdom Animal (Scientific Procedures) Act of 1986.", "Rat cortices are placed in an ice-cold Dissecting buffer (described in Materials).", "Wash five times wit... |
49,104 | Potentially inappropriate prescriptions of antipsychotics for patients with dementia | 1 | dx.doi.org/10.17504/protocols.io.bt7qnrmw | https://www.protocols.io/view/potentially-inappropriate-prescriptions-of-antipsy-bt7qnrmw | Manuel Machado, Jorge Machado Alba, Luis Valladales-REstrepo | TITLE: Potentially inappropriate prescriptions of antipsychotics for patients with dementia
AUTHORS: Manuel Machado, Jorge Machado Alba, Luis Valladales-REstrepo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Potentially inappropriate prescriptions of antipsychoti... | [] |
76,750 | Extraction of Yeast High-Molecular-Weight Genomic DNA | 4 | dx.doi.org/10.17504/protocols.io.rm7vzb1b4vx1/v1 | https://www.protocols.io/view/extraction-of-yeast-high-molecular-weight-genomic-cn7nvhme | Lois L. Hoyer | TITLE: Extraction of Yeast High-Molecular-Weight Genomic DNA
AUTHORS: Lois L. Hoyer
[DESCRIPTION]
This protocol is used for extraction of high-molecular-weight genomic DNA from yeast cells such as Candida albicans. We have used the protocol successfully for other fungal species such as Emydomyces testavorans that hav... | ["[Preparation of Reagents] The protocol requires four different solutions (called Solution 1 through Solution 4 below). Prepare each as directed and filter sterilize them. Solutions 1 through 3 are stored at 4 °C. Solution 4 is stored at room temperature.\n\n \n\nThe recipes below assume that you have various stock so... |
null | null | null | dx.doi.org/10.17504/protocols.io.eqfbdtn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
One-step growth curves are used to make determinations about the life cycle of a virus on a particular host. By following a virus infection during one life cycle phase of host a growth curve can be constructed and the burst size can be calculated.
[BEFORE_START]
<p>Before perf... | [] |
96,197 | Tissue Procurement SOP - Discarded Human Skin | 0 | dx.doi.org/10.17504/protocols.io.j8nlko7bwv5r/v1 | https://www.protocols.io/view/tissue-procurement-sop-discarded-human-skin-c97dz9i6 | Alberto Pappalardo, Rolando Perez-Lorenzo, Angela Christiano | TITLE: Tissue Procurement SOP - Discarded Human Skin
AUTHORS: Alberto Pappalardo, Rolando Perez-Lorenzo, Angela Christiano
[DESCRIPTION]
The purpose of this procedure is to provide instructions to clinicians, researchers and support staff for the collection and handling of human skin tissue. Specifically, this documen... | ["[Tissue Source] Discarded neonatal foreskin: \nThe neonatal human foreskin is a leftover of circumcision surgeries, which would otherwise be discarded.\n \nDiscarded adult skin:\nThe adult human skin is a leftover of reconstructive surgeries, which would otherwise be discarded.", "[Sample processing:] Samle collectio... |
null | null | null | dx.doi.org/10.17504/protocols.io.rk3d4yn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<p>This protocol was developed at this laboratory but had not been previously published.</p>
<p>The protocol aims explicitly to amplify hepatitis A virus (HAV) strains and not other virus species. The ... | [] |
39,948 | Reverse transcription with SuperScript VI VILO | 4 | null | https://www.protocols.io/view/reverse-transcription-with-superscript-vi-vilo-bi9kkh4w | Molly Moynihan | TITLE: Reverse transcription with SuperScript VI VILO
AUTHORS: Molly Moynihan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is from Invitrogen.</div><div class = "text-block"><span style = ":;"> </span><a href="https://www.thermofisher.com/order/catalog/product/11766050#/11766050" st... | ["[DNase Digestion]\nCheck for DNA contamination in RNA by performing PCR with universal primers (16S or 18S) (see PCR protocol). Doing the PCR first will help avoid unnecessary DNase use and wasting RNA.ORProceed directly to DNase treatment without initial check.", "[DNase Digestion]\nGently mix and incubate samples a... |
26,541 | UC Davis - Massons Trichrome | null | dx.doi.org/10.17504/protocols.io.56mg9c6 | null | Jennifer Rutkowsky | TITLE: UC Davis - Massons Trichrome
AUTHORS: Jennifer Rutkowsky
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Massons trichrome staining is used for detection of collagen fibers in tissues such as skin, heart, etc. o... | ["Hydrate to water.", "Place in Bouins for one hour in 55 degree oven or overnight at room temperature if needed.", "Wash well in running water until yellow is gone.", "Stain in Weigerts Hematoxylin for 10-20 minutes.", "Wash well in running water for 10 minutes. Rinse distilled water.", "Place slides in Biebrich Scarl... |
91,296 | DNA Concentration Protocol | 1 | dx.doi.org/10.17504/protocols.io.bp2l6xprklqe/v1 | https://www.protocols.io/view/dna-concentration-protocol-c5d8y29w | chyanne.rosenbaum | TITLE: DNA Concentration Protocol
AUTHORS: chyanne.rosenbaum
[DESCRIPTION]
This protocol is how the Eagle Fish Genetics Lab takes extracted DNA in a 96 well plate and concentrates it to five times the initial concentration.
[GUIDELINES]
This creates a 5x concentration.
[STEPS]
SECTION: Section 1 - Prepare Your Pla... | ["[Section 1 - Prepare Your Plate] Supplies needed per concentration plate:\na. Corresponding Initial DNA Extraction plate(s) \nb. low profile non-skirted PCR tray\nc. p200 pipette (green) set to 25 µL\nd. p200 pipette (green) tips\ne. Nexttec sealing tape (clear plastic seal)", "[Section 1 - Prepar... |
55,877 | SM buffer | 4 | dx.doi.org/10.17504/protocols.io.b2tdqei6 | https://www.protocols.io/view/sm-buffer-b2tdqei6 | Frej Larsen | TITLE: SM buffer
AUTHORS: Frej Larsen
[DESCRIPTION]
SM buffer is used for diluting and storing bacteriophages.
SM buffer contains:
200 mM NaCl2
10 mM MgSO4
50 mM Tris-HCl, pH 7.5
For storage at -20 or -80° C, 15% glycerol can be added before autoclaving.
The buffer can keep at room temperature for several months... | ["Weigh off 11.7 gNaCl2 and 1.4 gMgSO4 and add to a clean 1L blue cap flask or other autoclave safe container.", "Measure 50 mL Tris-HCl and add to the flask.", "Measure 950 mL demineralized water and add to the flask.", "Screw the cap on tight and mix thoroughly for 30 s.", "Loosen the cap and autoclave.", "If there a... |
28,929 | Simultaneous fitting of sigmoid curves in Excel (Excel Solver Add-in) | null | dx.doi.org/10.17504/protocols.io.8g9htz6 | null | Kenji Ohgane | TITLE: Simultaneous fitting of sigmoid curves in Excel (Excel Solver Add-in)
AUTHORS: Kenji Ohgane
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Sometimes it is desirable to fit sigmoid curves with shared parameters against several data sets. Such simultaneous fitting procedure is available ... | ["Download the following excel file.The file contains a sheet set up for simultaneous sigmoidal fitting with two set of example data, for which you can test the fitting procedure.", "Open the excel file, and enter your data set in the two set of \"x\" and \"y\" columns. Delete unnecessary data.\nThe data in the \"x\" c... |
94,160 | Intracardiac perfusion and rat brain fixation for immunohistochemistry | 4 | dx.doi.org/10.17504/protocols.io.yxmvm34w9l3p/v1 | https://www.protocols.io/view/intracardiac-perfusion-and-rat-brain-fixation-for-c77qzrmw | mariangela.massarocenere | TITLE: Intracardiac perfusion and rat brain fixation for immunohistochemistry
AUTHORS: mariangela.massarocenere
[DESCRIPTION]
Protocol for rat brain processing to perform parallel histological analysis in one hemisphere and high dimensional flow cytometry in the other.
[STEPS]
SECTION: 1. Animal sacrifice
1. Befor... | ["[1. Animal sacrifice] Before starting, fill the tube of a peristaltic pump with ice-cold 1X PB solution", "[1. Animal sacrifice] Deeply anesthetize the animal. Check for no reflexes and slower breathing", "[1. Animal sacrifice] Cut the animal's thorax and perfuse the animal through the left ventricle with ice-cold 1X... |
50,305 | Study Procedures (Part 6 of Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus) | 1 | dx.doi.org/10.17504/protocols.io.bvc9n2z6 | https://www.protocols.io/view/study-procedures-part-6-of-safety-and-efficacy-of-bvc9n2z6 | Stephen.Gitelman , Jeffrey A. Bluestone | TITLE: Study Procedures (Part 6 of Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus)
AUTHORS: Stephen.Gitelman , Jeffrey A. Bluestone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is Part 6 of "Safety and Efficacy of Imatinib for Preservi... | ["[Random Assignment]\nParticipants who sign the informed consent and meet the eligibility criteria will be randomly assigned in a 2:1 ratio to either the experimental or control group. A central automated randomization system will be used for treatment assignment and to create a unique identifier for each new study pa... |
36,722 | Cell harvest and RNA prep using Qiagen RNAeasy Kit | 1 | null | https://www.protocols.io/view/cell-harvest-and-rna-prep-using-qiagen-rnaeasy-kit-bf4sjqwe | Katrin Blondrath | TITLE: Cell harvest and RNA prep using Qiagen RNAeasy Kit
AUTHORS: Katrin Blondrath
[STEPS]
?. [Cell harvesting and RNA extraction ]
Aspirate medium from 6 well plates, wash once with PBS
?. [Cell harvesting and RNA extraction ]
Add lysis buffer containig freshly added beta-mercaptoethanol to each well (lysis buffer ... | ["[Cell harvesting and RNA extraction ]\nAspirate medium from 6 well plates, wash once with PBS", "[Cell harvesting and RNA extraction ]\nAdd lysis buffer containig freshly added beta-mercaptoethanol to each well (lysis buffer provided in RNAeasy kit)\n500 µl\nBeta-mercaptoethanol should be added freshly every time pe... |
24,060 | Mammalian Cell Culture: Subculturing | null | dx.doi.org/10.17504/protocols.io.3q4gmyw | null | Kenneth Schackart | TITLE: Mammalian Cell Culture: Subculturing
AUTHORS: Kenneth Schackart
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details how to subculture/passage nearly confluent mammalian cells grown in a tissue culture flask.</div></div>
[STEPS]
?. [Assess Cell Confluency]
Under light micros... | ["[Assess Cell Confluency]\nUnder light microscope, look at the cells and assess level of confluency. This is how you will determine the need to subculture.\nConfluency can be estimated by evaluating the percentage of surface covered by cells.", "[Wash Cells]\nUsing serological pipette, add DPBS to flask. [ for T-25]\... |
52,119 | Lysosomal membrane integrity | 4 | dx.doi.org/10.17504/protocols.io.bw5xpg7n | https://www.protocols.io/view/lysosomal-membrane-integrity-bw5xpg7n | Veerle Baekelandt | TITLE: Lysosomal membrane integrity
AUTHORS: Veerle Baekelandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Lysosomal membrane integrity </span></div></div>
[STEPS]
?. seed cells (SHSY-5Y) for 24:00:00 in a 12 well plate at a density of 1 x 105 cells per well
?... | ["seed cells (SHSY-5Y) for 24:00:00 in a 12 well plate at a density of 1 x 105 cells per well", "incubate cells with 5 µg/ml acridine orange (disolved in media) for at\n37 °C", "discard medium and wash cells with of 1X PBS. Add fresh medium to the wells.\n1 mL", "treat cells with SPM (different concentrations: 0.001... |
null | null | null | dx.doi.org/10.17504/protocols.io.hneb5be | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
26,990 | Direct cDNA synthesis and pre-amplification of single embryos for RT-PCR | null | dx.doi.org/10.17504/protocols.io.6knhcve | null | Yao Xiao, Peter Hansen | TITLE: Direct cDNA synthesis and pre-amplification of single embryos for RT-PCR
AUTHORS: Yao Xiao, Peter Hansen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol allows gene expression analysis within single embryos on multiple targets in either conventional real-time PCR or using the Fl... | [] |
97,409 | GeoMx Digital Spatial Profiler (DSP) Protocol v2 - University of Minnesota TMCs | 0 | dx.doi.org/10.17504/protocols.io.8epv5r7k6g1b/v2 | https://www.protocols.io/view/geomx-digital-spatial-profiler-dsp-protocol-v2-uni-dbc92iz6 | Laura J Niedernhofer, David A Bernlohr | TITLE: GeoMx Digital Spatial Profiler (DSP) Protocol v2 - University of Minnesota TMCs
AUTHORS: Laura J Niedernhofer, David A Bernlohr
[DESCRIPTION]
The nanoString GeoMx‱ Digital Spatial Profiler (DSP) is a platform that allows high-plex profiling at the protein and RNA level, providing spatial and temporal assessment... | ["[Slide Preparation] GeoMx DSP Manual Slide Preparation User Manual", "[ROI Acquisition] GeoMx DSP Instrument User Manual", "[Library Preparation] GeoMx DSP NGS Readout User Manual", "[FASTQ Generation] BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq version 2.20.0"] |
97,013 | Analyze Repeats | 0 | dx.doi.org/10.17504/protocols.io.dm6gpzeyjlzp/v1 | https://www.protocols.io/view/analyze-repeats-dayv2fw6 | Karina Jhingan | TITLE: Analyze Repeats
AUTHORS: Karina Jhingan
[DESCRIPTION]
A protocol that uses Homer's analyzeRepeats, findGo, and FindMotifsGenome on the output of bowtie2 run on data using different pre-treatment conditions (LSD1i, TGFb, and LDS1i+TGFb) at 3 different timepoints (day 0, 8, 16).
[STEPS]
SECTION: Introduction
1. ... | ["[Introduction] This linux analysis is using Homer, linked below. Here we are only focusing on the H3K9me3 histone for the human genome to compare different pre treatment conditions at different time points. http://homer.ucsd.edu/homer/ngs/analyzeRNA.html", "[Load modules] I have found that the order that modules is... |
82,746 | Cell surface biotinylation | 4 | dx.doi.org/10.17504/protocols.io.x54v9d9o4g3e/v1 | https://www.protocols.click/view/cell-surface-biotinylation-cu22wyge | rosanne.wouters, Peter Vangheluwe | TITLE: Cell surface biotinylation
AUTHORS: rosanne.wouters, Peter Vangheluwe
[DESCRIPTION]
The protocol describes cell surface biotinylation to identify plasma membrane localized proteins in cell culture via western blotting.
[STEPS]
1. grown cells until 70-80% confluency in 10cm dish
3. incubate cells for 30 min c ... | ["grown cells until 70-80% confluency in 10cm dish", "incubate cells for 30 min c on ice with PBS containing 2.5 mg/ml Sulfo-NHS-SS-biotin (Pierce).", "stop the biotinylation reaction by washing 3 times for 5min with quenching solution (0.5% BSA and 100 mM glycine in PBS).", "collect cells by scraping in PBS and centri... |
63,617 | Optimum Keto Pills Reviews [Official-2022] Ratings & Results! | 1 | dx.doi.org/10.17504/protocols.io.3byl4bd58vo5/v1 | https://www.protocols.io/view/optimum-keto-pills-reviews-official-2022-ratings-a-cac9saz6 | Optimum Keto | TITLE: Optimum Keto Pills Reviews [Official-2022] Ratings & Results!
AUTHORS: Optimum Keto
[DESCRIPTION]
Optimum Keto
[STEPS]
1. Optimum Keto Pills
Optimum Keto Pills:- The Ketogenic diet is a huge worry for the overwhelming majority of people's human well-being and life span. Notwithstanding, they accept it is p... | ["Optimum Keto Pills\nOptimum Keto Pills:- The Ketogenic diet is a huge worry for the overwhelming majority of people's human well-being and life span. Notwithstanding, they accept it is protected and could be extra powerful than other standard methods, like dietary plans or items.\n\nKeto pills, running against the no... |
null | null | null | dx.doi.org/10.17504/protocols.io.s4cegsw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Successful transformation of <em>Phaeodactylum tricornutum </em>(CMP632) by electroporation was achieved based on a modified version of Zhang & Hu (2014). </p>
[BEFORE_START]
<p>Materials needed for transformation </p>
<ul... | [] |
50,494 | Q5® Mastermix PCR-OpenPlast | 1 | null | https://www.protocols.io/view/q5-mastermix-pcr-openplast-bvi6n4he | yasoo | TITLE: Q5® Mastermix PCR-OpenPlast
AUTHORS: yasoo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for PCR with Q5® High-Fidelity 2X Master Mix (M0492)</div></div>
[STEPS]
?. Set up the following reaction on ice: ABCD12Q5 High-Fidelity 2X Master Mix5 µl10 µl1X310 µM Forward Primer... | ["Set up the following reaction on ice: ABCD12Q5 High-Fidelity 2X Master Mix5 µl10 µl1X310 µM Forward Primer0.5 µl1 µl0.5 µM410 µM Reverse Primer0.5 µl1 µl0.5 µM5Template DNAvariablevariable6Nuclease-Free Waterto 10 µlto 20 µl\nABCD12Q5 High-Fidelity 2X Master Mix5 µl10 µl1X310 µM Forward Primer0.5 µl1 µl0.5 µM410 µM ... |
69,944 | cDNA synthesis | 4 | dx.doi.org/10.17504/protocols.io.kqdg3948qg25/v1 | https://www.protocols.io/view/cdna-synthesis-cgiytufw | miquel.vila | TITLE: cDNA synthesis
AUTHORS: miquel.vila
[DESCRIPTION]
High-Capacity cDNA Reverse Transcription Kits(#4368814, Applied Biosystems - ThermoFisher)
[STEPS]
SECTION: cDNA synthesis
1. Prepare the 2X master mix (ul per sample):
- 2ul RT Buffer
- 0.8ul 25X dNTP Mix(100mM)
- 2ul Random primers
- 1ul Multiscribe Reverse... | ["[cDNA synthesis] Prepare the 2X master mix (ul per sample):\n- 2ul RT Buffer\n- 0.8ul 25X dNTP Mix(100mM)\n- 2ul Random primers\n- 1ul Multiscribe Reverse Transcriptase\n- 4.2ul Nuclease free water", "[cDNA synthesis] Mix the 2X master mix (10ul) with the RNA samples (10ul) and mix by pippeting", "[cDNA synthesis] Ce... |
101,845 | Fixation and immunocytochemistry (fluorescence) in PFF-treated cultures | 0 | dx.doi.org/10.17504/protocols.io.x54v922q4l3e/v1 | https://www.protocols.io/view/fixation-and-immunocytochemistry-fluorescence-in-p-dfpv3mn6 | Maria Iuliano | TITLE: Fixation and immunocytochemistry (fluorescence) in PFF-treated cultures
AUTHORS: Maria Iuliano
[DESCRIPTION]
This protocol outlines methods used for the preparation of in vitro models for fluorescent imaging including primary rat and mouse hippocampal cultures treated with PFFs.
[BEFORE_START]
Please read safe... | ["[Fixation and Immunocytochemistry (Fluorescence)] In a cell culture hood remove media and wash briefly with 1x PBS.", "[Fixation and Immunocytochemistry (Fluorescence)] Fix using 4% PFA 4% sucrose in 1xPBS with gentle rocking/shaking.", "[Fixation and Immunocytochemistry (Fluorescence)] Wash 1x PBS. Repeat 3 times.",... |
16,211 | Hemolysis measurement of plasma/serum samples with Nanodrop ND1000 | null | dx.doi.org/10.17504/protocols.io.t3teqnn | null | Hetty Helsmoortel | TITLE: Hemolysis measurement of plasma/serum samples with Nanodrop ND1000
AUTHORS: Hetty Helsmoortel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to measure hemolysis on plasma or serum samples. </div></div>
[STEPS]
?. [Before you start]
Place: room 120.042, 2nd floor... | ["[Before you start]\nPlace: room 120.042, 2nd floor MRB1Aliquot 3 ul plasma sample in a separate tube (can be left at room temperature)Open sample arm and clean sample pedestal with an ethanol-dipped swab (dust-free). Swabs are standing next to the machine.Clean the sample pedestal with a dry swab (dust-free).", "[Pre... |
105,632 | Mycoplasma Removal Treatment Protocols | 1 | dx.doi.org/10.17504/protocols.io.kxygx34pkg8j/v2 | https://www.protocols.io/view/mycoplasma-removal-treatment-protocols-djd84i9w | Carolina Lopez | TITLE: Mycoplasma Removal Treatment Protocols
AUTHORS: Carolina Lopez
[DESCRIPTION]
This protocol describes three mycoplasma removal procedures to be utilized depending on need. The protocols are described from milder/maintenance to the harshest option to be used in extreme cases of contamination when it is not possib... | ["[Mycoplasma Treatment Protocols] NOTES for effective treatment\n\nA. All treatment antibiotics should be added directly to the media at time of use. They should not be diluted or stored in media.\n\nB. For the most effective treatment, the cells should be trypsinized when the media is changed to release any mycoplas... |
37,750 | Degassing Reagents | 1 | dx.doi.org/10.17504/protocols.io.bg4wjyxe | https://www.protocols.io/view/degassing-reagents-bg4wjyxe | Allen Institute for Brain Science | TITLE: Degassing Reagents
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used for the degassing of critical reagents used in both the colorimetric and fluorescent in situ hybridization (ISH). This is crucial in eliminating hybridization bu... | [] |
42,111 | SapphireAmp PCR Master Mix -- CHEM 584 | 4 | dx.doi.org/10.17504/protocols.io.bmc7k2zn | https://www.protocols.io/view/sapphireamp-pcr-master-mix-chem-584-bmc7k2zn | Ken Christensen | TITLE: SapphireAmp PCR Master Mix -- CHEM 584
AUTHORS: Ken Christensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">SapphireAmp Fast PCR Master Mix contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent as a 2X premix. SapphireAmp Fast PCR M... | ["[Setup Reaction]\nTo a aliquot of SapphireAmp PCR Master Mix, add template (4 µl cleared lysate for colony PCR or of purified DNA for typical PCR), forward and reverse primers to a final concentration of . Adjust final volume to with nuclease free water or autoclaved water. AB1Sapphire Master Mix12.5 ul (pre-aliq... |
30,335 | iPSC Differentiation | 1 | null | https://www.protocols.io/view/ipsc-differentiation-9u7h6zn | Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying | TITLE: iPSC Differentiation
AUTHORS: Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol explains the iPSC differentiation of lines N... | ["[Day 1-3]\nPlate iPSCs at a density of 1.5 × 105cells per cm2 in a 12‐well plate pre-coated with growth-factor-reduced Matrigel.\nCulture medium should be changed daily, and gradually shifted from mTeSR1 to N2 (Thermo Fisher Scientific) in 3 days. The medium also contains , , and .", "[Day 1-3]\nTransfect iPSCs wi... |
null | null | null | dx.doi.org/10.17504/protocols.io.jxqcpmw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Permutation analysis for eyetracking study</p>
<p>Permutation Analysis: Two and three year old children use an incremental first-NP-as-agent bias to process transitive sentence</p>
[STEPS]
?. | [] |
45,846 | DNA extraction from plants | 1 | null | https://www.protocols.io/view/dna-extraction-from-plants-bqzwmx7e | Remco Stam | TITLE: DNA extraction from plants
AUTHORS: Remco Stam
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Widely used protocol to extract DNA from plant leaves.</div><div class = "text-block">Many versions circulate on the web, this is the version as we use it.</div><div class = "text-block">It works we... | ["In a 2 ml tube, add 800 uL of 1,5x CTAB and 1 ul of Beta-mercaptoethanol to the ground leaf materialIncubate 1 hour at 60-65 degrees (C)", "Cool at Room TempAdd 1 volume of Chloroform/Isoamylalkohol (24:1) mixtureMix on overhead shaker for 10 minutesCentrifuge at 3000 rmp for 25 minutes", "Transfer supernatant to a n... |
49,488 | Imaging intrinsic cardiac ganglia from the Yucatan pig | 1 | dx.doi.org/10.17504/protocols.io.bujqnumw | https://www.protocols.io/view/imaging-intrinsic-cardiac-ganglia-from-the-yucatan-bujqnumw | John Tompkins | TITLE: Imaging intrinsic cardiac ganglia from the Yucatan pig
AUTHORS: John Tompkins
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In process....</div></div>
[STEPS]
?. | [] |
18,104 | Joint angle calculation from optical markers and IMUs attached to the lower body | null | dx.doi.org/10.17504/protocols.io.vwye7fw | null | Wolfgang Teufl, Markus Miezal, Bertram Taetz, Michael Fröhlich, Gabriele Bleser | TITLE: Joint angle calculation from optical markers and IMUs attached to the lower body
AUTHORS: Wolfgang Teufl, Markus Miezal, Bertram Taetz, Michael Fröhlich, Gabriele Bleser
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The attached file contains a detailed description of the methods used in th... | [] |
39,204 | Nanomaterials UV-Vis measurement | 1 | null | https://www.protocols.io/view/nanomaterials-uv-vis-measurement-biickcaw | Ana Carrazco Quevedo, Emily J. Guggenheim, Sophie M. Briffa, Anastasios Papadiamantis | TITLE: Nanomaterials UV-Vis measurement
AUTHORS: Ana Carrazco Quevedo, Emily J. Guggenheim, Sophie M. Briffa, Anastasios Papadiamantis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SOP describes a sample preparation procedure for particle size measurements in gold NP suspensions. The procedu... | ["Sample PreparationVortex each AuNP stock suspension for 2 minutes.Dilute the Au suspensions 1:2. Pipette 0.5 mL of the Au stock suspension and 0.5 mL UPW 18.2 MΩcm to obtain a final volume of 1 mL and a mass concentration of 25 mg/L.", "Sample AnalysisSwitch the UV-Vis Spectrometer (Jenway 6800 Double Beam Spectro... |
50,188 | Dietary Sample for Phytochemical Assays | 4 | null | https://www.protocols.io/view/dietary-sample-for-phytochemical-assays-bu9knz4w | Alicia Rich | TITLE: Dietary Sample for Phytochemical Assays
AUTHORS: Alicia Rich
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">All of the methods and protocols for this project have been reviewed and approved by the Otterbein University Institutional Animal Care and Use Committee, Otterbein University's Enviro... | ["[Set aside food]\nFor one full day, each time you prepare the diet for our target animal, set aside one extra portion of food in a plastic bag or styrofoam crate. Keep this food in the same location or under the same conditions as you would just before you feed it to the primate.\nOur goal will be to extract chemical... |
50,935 | Protocol for Creating Major Ion Solutions for Freshwater Systems | 1 | dx.doi.org/10.17504/protocols.io.bvyxn7xn | https://www.protocols.io/view/protocol-for-creating-major-ion-solutions-for-fres-bvyxn7xn | Malcolm Barnard | TITLE: Protocol for Creating Major Ion Solutions for Freshwater Systems
AUTHORS: Malcolm Barnard
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Several experimental designs require serial dilutions of site water. To perform dilutions without the use of prefiltered water or without the additi... | ["[Find major ion composition of the freshwater body]\nIdentify the elemental concentrations of Si4+, Ca2+, Mg2+, Na+, K+, Cl-, and SO42- in the freshwater system for which you wish to create a major ion solution. Note: USGS reports are helpful for water bodies in the United States, but major ion compositions from elem... |
106,502 | KAPP-Sen TMC: Tissue blocks paraffin embedding by JAX Histology Services (RNase-free) | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj9z2lx1/v1 | https://www.protocols.io/view/kapp-sen-tmc-tissue-blocks-paraffin-embedding-by-j-dj9e4r3e | Anne Marchini, Tim Adams, Elaine Bechtel, Lesley Bechtold | TITLE: KAPP-Sen TMC: Tissue blocks paraffin embedding by JAX Histology Services (RNase-free)
AUTHORS: Anne Marchini, Tim Adams, Elaine Bechtel, Lesley Bechtold
[DESCRIPTION]
This protocol describes embedding of formalin-fixed tissues in paraffin (FFPE). Paraffin-embedded blocks are then kept at 4 °C and protected from... | ["Wear prescribed PPE throughout all of the procedures described in this document. Change gloves if they get wet.", "Histology does not process tissue by hand for RNase free requests. The usual tissue processing unit (Leica Histocore Peloris 3) is used. Use RNase free best practices when handling the tissue (wear glove... |
91,258 | E8 media production | 4 | dx.doi.org/10.17504/protocols.io.6qpvr32ppvmk/v1 | https://www.protocols.io/view/e8-media-production-c5c2y2ye | Valeria Fernandez Vallone, Lyn Healy, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Lisa Pavinato, Fatma Visal FVO OKUR, Harald Stachelscheid | TITLE: E8 media production
AUTHORS: Valeria Fernandez Vallone, Lyn Healy, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Lisa Pavinato, Fatma Visal FVO OKUR, Harald Stachelscheid
[DESCRIPTION]
This protocol describes the procedure to prepare E8 Supplement and complete E8 medium for hPSC maintenance culture.
[GUIDELI... | ["[Reagent Storage Requirements Upon Delivery]", "[Preparation of reagents] L-Ascorbic acid 2-phosphate", "[E8 Supplement] Assembly of E8 Supplement", "[Supplemented E8 media] Preparation of supplemented E8 media (0.5 L)", "[Preparation of reagents] Insulin, Transferrin and Sodium Selenite", "[Preparation of reagents] ... |
100,974 | 18S rRNA-Gene Metabarcoding Library Prep: Dual-PCR Method | 1 | dx.doi.org/10.17504/protocols.io.rm7vzjjjxlx1/v1 | https://www.protocols.io/view/18s-rrna-gene-metabarcoding-library-prep-dual-pcr-deun3eve | rute.carvalho Carvalho, Colleen Kellogg, Matt Lemay | TITLE: 18S rRNA-Gene Metabarcoding Library Prep: Dual-PCR Method
AUTHORS: rute.carvalho Carvalho, Colleen Kellogg, Matt Lemay
[DESCRIPTION]
This protocol is used for eDNA metabarcoding of the 18S SSU rRNA Gene (Balzano et al 2015) using Pair-End Illumina Miseq. Sequencing. As part of the Hakai Institute Ocean Observin... | ["[Preparations] Ensure that the laboratory is appropriately configured and that staff has appropriate training. See \"Guidelines\" for more information. Pay attention to the separation of pre and post-PCR spaces and equipment.", "[Triplicate PCR Amplification (1st PCR)] Preparations\n\n \n\n Reagents:\n (Or equal)\n ... |
53,616 | DNA Extraction from Sterivex Filters | 4 | dx.doi.org/10.17504/protocols.io.bykqpuvw | https://www.protocols.io/view/dna-extraction-from-sterivex-filters-bykqpuvw | Christopher Neil Thornton, William Brazelton | TITLE: DNA Extraction from Sterivex Filters
AUTHORS: Christopher Neil Thornton, William Brazelton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Modified 2015 by the Brazelton Lab from protocols by Rika Anderson, Colleen Kellogg, Julie Huber, and Byron Crump. Incorporated some recommendations from ... | ["[Hot Lysis]\nAdd 1.4 mL of DEB to each Sterivex with syringe and needle. Position the needle just below the mouth of the Sterivex so that it does not come back out the top. Do not fill to the top – stop when solution covers white filter. Possible Stopping Point. Store at 20ºC", "[Hot Lysis]\nPossible Stopping Point.... |
99,556 | HEPES-Phosphate Medium, Suitable for Studies of Trace Element Nutrition in Photoautotrophic and Heterotrophic Auxenochlorella protothecoides. | 0 | dx.doi.org/10.17504/protocols.io.kxygxyzdzl8j/v1 | https://www.protocols.io/view/hepes-phosphate-medium-suitable-for-studies-of-tra-ddgc23sw | Dimitrios Camacho, Charles Perrino, Sabeeha Merchant | TITLE: HEPES-Phosphate Medium, Suitable for Studies of Trace Element Nutrition in Photoautotrophic and Heterotrophic Auxenochlorella protothecoides.
AUTHORS: Dimitrios Camacho, Charles Perrino, Sabeeha Merchant
[DESCRIPTION]
This protocol describes a method for preparing a defined medium for phototrophic and heterotr... | ["[Wash all culture flasks, stock solution containers, and graduated cylinders with 6 M HCl] Do not use glass containers to store stock solutions as glass will leach metal contaminants. Use new (previously unused) wide-mouth polypropylene / translucent high-density polyethylene (HDPE) bottles. For macronutrient stock s... |
43,707 | DNA-Extraction-ClostridiaProject | 1 | null | https://www.protocols.io/view/dna-extraction-clostridiaproject-bnw3mfgn | Tobias Wandt | TITLE: DNA-Extraction-ClostridiaProject
AUTHORS: Tobias Wandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol used for DNA-Extraction of Clostridia in my project. </div><div class = "text-block">DNA-Kit used is GenFind V3 by Beckmann/Coulter.</div><div class = "text-block">Some steps used f... | ["[Growing and Preparing Clostridia]\nInoculate Clostridia in 10ml TSB-Broth, incubating at least 48h in anaerobe atmosphere at 37°C\n[TSB broth]\n[at 37°C anaerobic]", "[Growing and Preparing Clostridia]\nTake 2ml of Clostridia TSB-Broth and centrifuge for 15min at 3,000g, after that remove supernatant\n[Clostridia TS... |
71,189 | Microscopy-based measurements of p62 recruitment in HeLa | 4 | dx.doi.org/10.17504/protocols.io.3byl4jkdjlo5/v1 | https://www.protocols.io/view/microscopy-based-measurements-of-p62-recruitment-i-chrvt566 | Felix Kraus | TITLE: Microscopy-based measurements of p62 recruitment in HeLa
AUTHORS: Felix Kraus
[DESCRIPTION]
Protocol for microscopy-based measurements of p62 recruitment in HeLa
[STEPS]
SECTION: Seeding of HeLa cells
1. Wash HeLa cells expressing doxycycline-inducible Parkin with 1x PBS
SECTION: Seeding of HeLa cells
2. Add T... | ["[Seeding of HeLa cells] Wash HeLa cells expressing doxycycline-inducible Parkin with 1x PBS", "[Seeding of HeLa cells] Add Trypsin to cells for 5 min and incubate at 37°C to dissociate cells from plastic well", "[Seeding of HeLa cells] Resuspend cells in 1 mL DMEM media", "[Seeding of HeLa cells] Count cells", "[Seed... |
34,131 | Heterologous protein expression in E. coli | 1 | dx.doi.org/10.17504/protocols.io.bdjti4nn | https://www.protocols.io/view/heterologous-protein-expression-in-e-coli-bdjti4nn | Diep Ganguly, Timothy Rhodes, Nay Chi Khin, Estee E Tee, Kai Xun Chan | TITLE: Heterologous protein expression in E. coli
AUTHORS: Diep Ganguly, Timothy Rhodes, Nay Chi Khin, Estee E Tee, Kai Xun Chan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for recombinant protein expression in E. coli for protein purification and subsequent enzyme assays, protein cryst... | ["[Prepare buffers]\nBuffer recipesAuto-induction TB medium (alternatively, liquid LB media for IPTG induction)5 g/L yeast extract20 g/L tryptone85.5 mM NaCl (5 g/L)22 mM KH2PO4 (2.99 g/L)42 mM Na2HPO4 (5.96 g/L)Supplement fresh: 0.6% glycerol, 0.05% glucose, 0.2% lactose10X PBSDissolve the following in 800 mL H2O:80 g... |
36,974 | Long Primer PCR (for Trypanosoma brucei) | null | dx.doi.org/10.17504/protocols.io.bgcnjsve | https://www.protocols.io/view/long-primer-pcr-for-trypanosoma-brucei-bgcnjsve | Alex Zegarra | TITLE: Long Primer PCR (for Trypanosoma brucei)
AUTHORS: Alex Zegarra
[STEPS]
?. PRC Mix 1 uL pPOT (25 ng/uL)0.2 mM dNTPs1 uM for primer 1 uM rev primer 1 uL PCR grade DMSO 5uL 10x buffer 2 (Roche) XX uL ddH20 for total volume of 49 uLAdd 1 uL Expand High Fidelity polymerase (Roche) once mixture has reached 94 C.
?... | ["PRC Mix 1 uL pPOT (25 ng/uL)0.2 mM dNTPs1 uM for primer 1 uM rev primer 1 uL PCR grade DMSO 5uL 10x buffer 2 (Roche) XX uL ddH20 for total volume of 49 uLAdd 1 uL Expand High Fidelity polymerase (Roche) once mixture has reached 94 C.", "Maintain procyclic form SMOX P9 cells [31] between 1x106 -1x107 cells ml-1 for ... |
18,334 | 5/12 | null | dx.doi.org/10.17504/protocols.io.v56e89e | null | Tasty Testy | TITLE: 5/12
AUTHORS: Tasty Testy
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.irtcd6n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289).
[GUIDELINES]
<p><strong>Dephosphorylation of 5' -ends of DNA in Restriction Enzyme Reaction</strong></p>
<ul>
<li>The phosphate can be added directly into the digestion react... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.drp55m | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol provides a detailed description of how to design and perform BONCAT experiments using two different bioorthogonal amino acids, <span class="s1">L</span>-azidohomoalanine (AHA) and <span class="s1">L</span>-homopropargylglycine (HPG), which are both surrogates of... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.f7ibrke | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Cara memasukkan foto mentah (<em>raw</em>) ke dalam <em>photo frame</em> yang sudah disiapkan dalam format PowerPoint, agar siap dipublikasi di media sosial dan situs web.</p>
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
95,311 | Total and PS129 aSyn levels using Western Blot | 4 | dx.doi.org/10.17504/protocols.io.n92ldmk78l5b/v1 | https://www.protocols.io/view/total-and-ps129-asyn-levels-using-western-blot-c9bpz2mn | daniel.dautan daniel, Wojciech Paslawski, Per Svenningsson | TITLE: Total and PS129 aSyn levels using Western Blot
AUTHORS: daniel.dautan daniel, Wojciech Paslawski, Per Svenningsson
[DESCRIPTION]
Measure total and ps129 alpha-synuclein in mouse plasma.
[STEPS]
1. Dilute plasma samples 10-fold in MilliQ water.
2. Mix the sample in a 1:1 ratio with 20 mM TCEP to reduce disulf... | ["Dilute plasma samples 10-fold in MilliQ water.", "Mix the sample in a 1:1 ratio with 20 mM TCEP to reduce disulfide bridges, and then 1:1 with a denaturing loading buffer (50 mM Tris-HCl, 70 mM Tris, 1% lithium dodecyl sulphate (LDS), 5%\nglycerol, 0.25mM EDTA, 0.11mM SERVA Blue G-250, 0.0875mM Phenol Red, pH-8.5). B... |
64,385 | Fluxactive Complete What are Customers Saying? Know the Truth! | 3 | dx.doi.org/10.17504/protocols.io.dm6gpbq61lzp/v1 | https://www.protocols.io/view/fluxactive-complete-what-are-customers-saying-know-ca49sgz6 | Fluxactive | TITLE: Fluxactive Complete What are Customers Saying? Know the Truth!
AUTHORS: Fluxactive
[DESCRIPTION]
Fluxactive Complete
• Product Name - Fluxactive Complete
• Composition - Natural Organic Compound
• Side-Effects - NA
• Price - Visit Official Website
• Supplement Type - Pills
• Official Website - www.fluxac... | [] |
72,812 | Use and efficiency of morpholinos in Neotropical tadpole brains | 4 | dx.doi.org/10.17504/protocols.io.yxmvm23y6g3p/v1 | https://www.protocols.io/view/use-and-efficiency-of-morpholinos-in-neotropical-t-cjckuiuw | Sarah C. Ludington, Julie Butler, Chloe Golde, Lauren A O'Connell | TITLE: Use and efficiency of morpholinos in Neotropical tadpole brains
AUTHORS: Sarah C. Ludington, Julie Butler, Chloe Golde, Lauren A O'Connell
[DESCRIPTION]
Antisense morpholinos are a common tool used to knockdown protein abundance in target tissues in fish and amphibians. However, protocols are largely limited to... | ["[Anesthesia Preparation] Mix 0.02g ethyl 3-aminobenzoate methanesulfonate (MS-222) and 0.08g sodium bicarbonate with 60 mL of tadpole water", "[Anesthesia Preparation] Store at 4C for up to one week", "[Injection Set-Up] Reconstitute morpholino solution (0.5 - 1.0 mM working concentration) in nuclease-free water", "[... |
65,251 | Scam Alert: ChillWell Portable AC Reviews 2022 #trending | 3 | dx.doi.org/10.17504/protocols.io.14egn713qv5d/v1 | https://www.protocols.io/view/scam-alert-chillwell-portable-ac-reviews-2022-tren-cbybspsn | tryProDentim | TITLE: Scam Alert: ChillWell Portable AC Reviews 2022 #trending
AUTHORS: tryProDentim
[DESCRIPTION]
Scam Alert: ChillWell Portable AC Reviews 2022 #trending
[STEPS] | [] |
41,490 | Culturing Chlamydomonas reinhardtii | 4 | null | https://www.protocols.io/view/culturing-chlamydomonas-reinhardtii-bkrskv6e | Joao Vitor Molino | TITLE: Culturing Chlamydomonas reinhardtii
AUTHORS: Joao Vitor Molino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocols describe the steps required for the growth of </span><span style = "font-style:italic;">Chlamydomonas reinhardtii</span><span> in liquid media. </span></div></d... | ["[Material]\nTAP media or other (How to prepare TAP media here)Erlenmeyer flaskOrbital shakerLight source", "[Inocullum]\n*Inoculation can be perfomed by scraping an agar plate containing Chlamydomonas reinhardtii or from another liquid cultureInnoculate the cells in an erlenmeyer containing media (Ex: containing ... |
44,462 | Designing gRNA using CRISPRdirect | 5 | null | https://www.protocols.io/view/designing-grna-using-crisprdirect-bpnnmmde | Gatesgibson | TITLE: Designing gRNA using CRISPRdirect
AUTHORS: Gatesgibson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is designed to help guide the user through the use of CRISPRdirect to design gRNA for CRISPR/Cas9 applications in the model mouse organism Mus musculus. The target gene for th... | ["[Software used]\nIn this protocol, the software used to generate both inputs and outputs are CRISPRdirect and Benchling", "[Software used]\nUsing Benchling, the user can easily access the genetic information needed to begin designing gRNA using the selected gRNA software.In this example, the gene in question is the I... |
65,884 | Tiger Woods CBD Gummies | 1 | dx.doi.org/10.17504/protocols.io.yxmvmnjyng3p/v1 | https://www.protocols.io/view/tiger-woods-cbd-gummies-ccj4suqw | bisena | TITLE: Tiger Woods CBD Gummies
AUTHORS: bisena
[DESCRIPTION]
This is an all-normal plant-based supplement to work on one's wellbeing and health every day by killing the issues that individuals habitually battle with
[STEPS]
SECTION: Tiger Woods CBD Gummies
1. ➢Product Review: — 🔶 Tiger Woods CBD Gummies
➢Used For ... | ["[Tiger Woods CBD Gummies] ➢Product Review: — 🔶 Tiger Woods CBD Gummies\n➢Used For —🔶Pain Relief\n➢Main Benefits: —🔶Improve Metabolism & Help in Pain Relief\n➢Composition: —🔶Natural Organic Compound\n➢Side-Effects: —🔶NA\n➢Rating: —🔶Overall rating: — ⭐⭐⭐⭐⭐ 5 out of 5\n➢Age range: ... |
null | null | null | dx.doi.org/10.17504/protocols.io.ejnbcme | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol outlines our analysis of the potential antibiotic resistance genes found within the skin virome. We start by visualizing the relative abundances of the top 10 antibiotic resistance categories (according to the CARD). We then quantify the number of unique antibiotic... | [] |
40,369 | ELISA for anti-SpA antibodies in hyper-immune egg whites. | 4 | dx.doi.org/10.17504/protocols.io.bjnrkmd6 | https://www.protocols.io/view/elisa-for-anti-spa-antibodies-in-hyper-immune-egg-bjnrkmd6 | Angel Justiz-Vaillant | TITLE: ELISA for anti-SpA antibodies in hyper-immune egg whites.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. The 96 well polystyrene microplate (U-shaped bottom) is coated with 500 ng of SpA (Sigma-Aldrich) in coating buffer for 4 h at 37oC.
?. The microplate is washed f... | ["The 96 well polystyrene microplate (U-shaped bottom) is coated with 500 ng of SpA (Sigma-Aldrich) in coating buffer for 4 h at 37oC.", "The microplate is washed four times with PBS-Tween-20 and blocked with 3% non-fat milk in PBS, 25 μl/well, 1h, RT.", "The microplate is washed four times again.", "Samples of egg whi... |
71,935 | HTTM : Transposon mutagenesis | 4 | dx.doi.org/10.17504/protocols.io.36wgq72n3vk5/v1 | https://www.protocols.io/view/httm-transposon-mutagenesis-cig7ubzn | Antoine Champie, Amélie De Grandmaison | TITLE: HTTM : Transposon mutagenesis
AUTHORS: Antoine Champie, Amélie De Grandmaison
[DESCRIPTION]
Part one of the HTTM protocol. A low-cost and high-throughput Tn-seq protocol.
This part cover the transposon mutagenesis and selection steps.
[BEFORE_START]
Per plate refers to the number of 96 well plates of target... | ["[Day 1] (1-A) Make a 15 mL LB (Diaminopimelic acid [Dap], Ampicillin [Amp], Spectinomycin [Spec]) pre-culture ( 2 mL per plate minimum) of the donor strain eAC494 and incubate with agitation at 37 °C overnight.", "[Day 1] (1-B) Prepare the 96 deep-well plates for conjugation :", "[Day 1] Preheat the deep-well plates ... |
null | null | null | dx.doi.org/10.17504/protocols.io.metc3en | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The basic decoding of MERFISH data—the identification of specific RNA species for each spot in the sample—consists of three basic steps. First, fluorescent spots must be identified in all images. Second, slight changes in the stage position between images of the same region f... | [] |
21,588 | Creation and maintenance of Ashworth outcrossed DGRP population | null | dx.doi.org/10.17504/protocols.io.zbuf2nw | null | Fergal Waldron, katy monteith, Pedro Vale | TITLE: Creation and maintenance of Ashworth outcrossed DGRP population
AUTHORS: Fergal Waldron, katy monteith, Pedro Vale
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Infomration about the creation and maintenance of Ashworth outcrossed DGRP population</span></di... | ["Information on creation and maintenance of Ashworth outcrossed DGRP populationFergal M Waldron, Katy M Monteith, Pedro F ValeThe Ashworth outcrossed DGRP population was founded by FMW on 15/10/14, and is derived from 113 inbred DGRP lines sampled from a wild population in Raleigh, NC, USA 1. The DGRP outcrossed popul... |
59,609 | High time-resolution of root tracking of Arabidopsis roots using SPIRO, SmartRoot and BackTracker | 4 | dx.doi.org/10.17504/protocols.io.81wgb6z5olpk/v1 | https://www.protocols.io/view/high-time-resolution-of-root-tracking-of-arabidops-b6fzrbp6 | Magdalena M Julkowska | TITLE: High time-resolution of root tracking of Arabidopsis roots using SPIRO, SmartRoot and BackTracker
AUTHORS: Magdalena M Julkowska
[DESCRIPTION]
This protocol describes an alternative way of analyzing SPIRO images for root growth of plants that are transferred to the treatment plates - and thus not imaged from th... | ["Collect your images caputred by SPIRO from the Raspberry Pi by going into the FileManager - and downloading your experiment:", "Pre-process your images for drifting using the SPIRO pre-process in ImageJ / Fiji - as described on SPIRO Assay github page here. This will result in all of your images being cropped and ALI... |
81,273 | In vitro transcription of guide RNAs and 5'-triphosphate removal | 1 | dx.doi.org/10.17504/protocols.io.n2bvjyp5vk5w/v17 | https://www.protocols.io/view/in-vitro-transcription-of-guide-rnas-and-5-39-trip-ctkzwkx6 | Mark Dewitt, Julia Wong, Beeke Wienert, Moritz F Schlapansky, Eric Aird | TITLE: In vitro transcription of guide RNAs and 5'-triphosphate removal
AUTHORS: Mark Dewitt, Julia Wong, Beeke Wienert, Moritz F Schlapansky, Eric Aird
[DESCRIPTION]
sgRNA template assembly, in vitro T7 transcription, and sgRNA column cleanup to remove 5'-triphosphate groups
[GUIDELINES]
The primers used are: o... | ["[Design sgRNA and order PCR oligos] Add the desired protospacer sequence to the T7FwdVarV2 oligo and order the oligo from your favorite oligonucleotide supplier. There are many programs available for protospacer design that attempt to optimize on- and/or off-target activity. Which program is most useful depends upon ... |
98,320 | Cryomiller SOP | 1 | dx.doi.org/10.17504/protocols.io.5qpvormjzv4o/v2 | https://www.protocols.io/view/cryomiller-sop-db9q2r5w | Lorenzo Lucherini | TITLE: Cryomiller SOP
AUTHORS: Lorenzo Lucherini
[DESCRIPTION]
Standard of procedure for cryomiller Retsch at the Soft Materials Laboratory (EPFL)
[STEPS]
SECTION: Refill the N2 dewar
4. Use two wrenches to unscrew the N2 pipe from the dewar. Firmly push downwards both wrenches at the same time.
SECTION: Refill th... | ["[Refill the N2 dewar] Use two wrenches to unscrew the N2 pipe from the dewar. Firmly push downwards both wrenches at the same time.", "[Refill the N2 dewar] Place the dewar in the lift and block the wheels. Pull the red strap in front of the door of the lift and send the lift to floor 0.", "[Refill the N2 dewar] Hand... |
67,275 | Sonovive Reviews – Consume The Supplement | 1 | dx.doi.org/10.17504/protocols.io.14egn7kqzv5d/v1 | https://www.protocols.io/view/sonovive-reviews-consume-the-supplement-cdxjs7kn | sonoviveget | TITLE: Sonovive Reviews – Consume The Supplement
AUTHORS: sonoviveget
[DESCRIPTION]
As per the authority site,Sonoviveis a high level regular equation planned explicitly to further develop ear wellbeing by working on hearing and ear cell working. The Ear Health Support Formula is made utilizing normal fixings that h... | [] |
61,945 | Power Keto Gummies Reviews! | 3 | dx.doi.org/10.17504/protocols.io.ewov1n37ygr2/v1 | https://www.protocols.io/view/power-keto-gummies-reviews-b8qzrvx6 | Power Keto Gummies Reviews | TITLE: Power Keto Gummies Reviews!
AUTHORS: Power Keto Gummies Reviews
[DESCRIPTION]
===>(SUPER OFFER) Click Here To Buy Now Power Keto Gummies Only From Its Official Website
[STEPS] | [] |
28,463 | ChroPack - ProteinA/G | null | dx.doi.org/10.17504/protocols.io.72phqdn | null | Alexandra Ehl, David Frommholz, Nadine Stefanczyk | TITLE: ChroPack - ProteinA/G
AUTHORS: Alexandra Ehl, David Frommholz, Nadine Stefanczyk
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Purification Guide for the Isolation of Antibodies with ChroPack Columns by DALEX Biotech.</span></div><div class = "text-block">W... | ["[What do you want to do?]\nDo you want to purify antibodies or sanitize your column?Please choose below.", "[Equilibration]\nConnect the column to your FPLC system. Set the flow rate to 1 bed volume per minute. Wash the column with 5 volumes deionized water (bed volume is written on the column).\nA dry column can be ... |
49,546 | Kujawinski Lab cleaning protocol | 1 | null | https://www.protocols.io/view/kujawinski-lab-cleaning-protocol-buminu4e | Krista Longnecker | TITLE: Kujawinski Lab cleaning protocol
AUTHORS: Krista Longnecker
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes lab cleaning procedure for glass and plastics.</div></div>
[STEPS]
?. [Kujawinski Lab cleaning protocol]
Add a small amount of diluted Citranox to hot water in ... | ["[Kujawinski Lab cleaning protocol]\nAdd a small amount of diluted Citranox to hot water in tub (less than into ~2 gallons of water).\n5 mL", "[Kujawinski Lab cleaning protocol]\nAdd dishes to the tub making sure every surface is covered with water.\nContainers with chunky bits should be scrubbed with a brush to remov... |
64,111 | DTS-TOT protocol | 1 | dx.doi.org/10.17504/protocols.io.n92ldzn88v5b/v1 | https://www.protocols.io/view/dts-tot-protocol-caupsevn | marcel.hinss , raphaelle.roy , Anke M Brock | TITLE: DTS-TOT protocol
AUTHORS: marcel.hinss , raphaelle.roy , Anke M Brock
[DESCRIPTION]
Cognitive Flexibility, the ability to adapt behavior and thought to the environment is one of the most important aspects of human cognition. A classical paradigm to study cognitive flexibility is the task-switching paradigm... | ["[Training Session] Participant Arrival", "[Training Session] Informing the Participant about the Nature of the Experiment", "[Training Session] Participant Arrival", "[Training Session] Colourblindness Test", "[Training Session] Signing of Information Sheet", "[Training Session] Signing of Informed Consent", "[Traini... |
50,345 | Quality Control and Quality Assurance (Part 11 of Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus) | 1 | dx.doi.org/10.17504/protocols.io.bvehn3b6 | https://www.protocols.io/view/quality-control-and-quality-assurance-part-11-of-s-bvehn3b6 | Stephen.Gitelman , Jeffrey A. Bluestone | TITLE: Quality Control and Quality Assurance (Part 11 of Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus)
AUTHORS: Stephen.Gitelman , Jeffrey A. Bluestone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is Part 11 of "Safety and Efficacy o... | [] |
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