id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
64,412 | Condor CBD Gummies (Scam or Legit?) Effective Formula or Cheap Brand? | 3 | dx.doi.org/10.17504/protocols.io.yxmvmnm46g3p/v1 | https://www.protocols.io/view/condor-cbd-gummies-scam-or-legit-effective-formula-ca54sg8w | fluxactive | TITLE: Condor CBD Gummies (Scam or Legit?) Effective Formula or Cheap Brand?
AUTHORS: fluxactive
[DESCRIPTION]
Official Website - Click Here for Condor CBD Gummies
Availability Of Condor CBD Gummies - Online On Website
Main Benefits - Support Pain Relief Without Any Side Effect
[STEPS] | [] |
102,393 | Magic Red Cathepsin B activity assay | 0 | dx.doi.org/10.17504/protocols.io.ewov192b2lr2/v1 | https://www.protocols.io/view/magic-red-cathepsin-b-activity-assay-df8z3rx6 | Razaul Karim | TITLE: Magic Red Cathepsin B activity assay
AUTHORS: Razaul Karim
[DESCRIPTION]
This protocol details the magic red Cathepsin B activity assay.
[STEPS]
SECTION: Cell Culture/Treatments
1. 0.5x10^4/ml of cells plated on coverslips.
SECTION: Cell Culture/Treatments
2. PFF-488 treatment.
SECTION: Cell Culture/Treatmen... | ["[Cell Culture/Treatments] 0.5x10^4/ml of cells plated on coverslips.", "[Cell Culture/Treatments] PFF-488 treatment.", "[Cell Culture/Treatments] PBS/trypsin (0.01%) wash.", "[Cell Culture/Treatments] 1x Magic Red solution for last 15 min at 37 °C in cell culture incubator.", "[Imaging] Live-Cell imaging.", "[Imaging... |
53,315 | 6M GITC | 4 | dx.doi.org/10.17504/protocols.io.bybbpsin | https://www.protocols.io/view/6m-gitc-bybbpsin | Jacquelina.Woods | TITLE: 6M GITC
AUTHORS: Jacquelina.Woods
[DESCRIPTION]
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA ... | ["Aseptically mix 7.09 g in sterile distilled or ultrapure water to 4.5 mL", "Bring total volume up to 10 mLwith additional sterile distilled or ultrapure water", "Store at 2-8 Room temperaturein the dark (or in light occluding tube)"] |
null | null | null | dx.doi.org/10.17504/protocols.io.u8iezue | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Protocol to recover plasmids from filter papers - copy from Addgene webpage (https://help.addgene.org/hc/en-us/articles/206127485-DNA-on-filter-paper-or-tube)
[STEPS]
?.
?.
?. | ["To recover the plasmid, use a clean razor blade to cut out one of the circles containing your DNA.", "{\"blocks\":[{\"key\":\"4tbep\",\"text\":\"Immerse the circle in of \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[{\"offset\":22,\"length\":1,\"key\":0}],\"data\":[]},{\"key\":\"cvr... |
null | null | null | dx.doi.org/10.17504/protocols.io.kaecsbe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocols for samping and shipping human skin swabs from clinics to lab.</p>
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
94,079 | Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections. | 1 | dx.doi.org/10.17504/protocols.io.q26g7pk1qgwz/v1 | https://www.protocols.io/view/protocol-for-preparing-post-mortem-tissue-for-intr-c747zqzn | Marta Gonzalez-Sepulveda, Thais Cuadros, Miquel Vila | TITLE: Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
AUTHORS: Marta Gonzalez-Sepulveda, Thais Cuadros, Miquel Vila
[DESCRIPTION]
Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections
[STEPS... | ["[Tissue Processing] Formalin-fixed paraffin-embedded tissue blocks from the pontine and midbrain regions were sectioned at 5µm, collected onto adhesive microscope slides and allowed to dry at room temperature for 24 hours.", "[Tissue Processing] Slides were incubated in the oven at 60°C for 30 minutes to melt the par... |
41,092 | SARS-CoV-2 detection with ApharSeq | 1 | dx.doi.org/10.17504/protocols.io.bkdcks2w | https://www.protocols.io/view/sars-cov-2-detection-with-apharseq-bkdcks2w | Daphna Strauss, Ayelet Rahat, Israa Sharkia, Alon Chappleboim, Miriam Adam, Daniel Kitsberg, Gavriel Fialkoff, Matan Lotem, Omer Gershon, Anna-Kristina Schmidtner, Esther Oiknine-Djian, Agnes Klochendler, Ronen Sadeh, Yuval Dor, Dana Wolf, Naomi Habib, Nir Friedman | TITLE: SARS-CoV-2 detection with ApharSeq
AUTHORS: Daphna Strauss, Ayelet Rahat, Israa Sharkia, Alon Chappleboim, Miriam Adam, Daniel Kitsberg, Gavriel Fialkoff, Matan Lotem, Omer Gershon, Anna-Kristina Schmidtner, Esther Oiknine-Djian, Agnes Klochendler, Ronen Sadeh, Yuval Dor, Dana Wolf, Naomi Habib, Nir Friedman
[D... | ["[Apharseq]\nPrepare poly dT beadsUse 5 µl poly dT beads/sampleWash beads twice in binding buffer: 2.1 Resuspend in binding buffer 2.2 Magnetize and remove buffer 3. Resuspend beads in 320 µl binding buffer", "[Apharseq]\nHybridization to beadsAdd 320 µl inactivated viral sample to 320 µl beads in binding b... |
null | null | null | dx.doi.org/10.17504/protocols.io.f48bqzw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 16">
<div class="layoutArea">
<div class="column">
<p>The ISOLATE II Biofluids RNA Kit is specially developed for the rapid phenol-free isolation of high quality total RNA from biofluids and viruses.</p>
<p> </p>
<p>The following protocol is two par... | [] |
59,614 | FLASH-seq Low-Amplification protocol | 4 | dx.doi.org/10.17504/protocols.io.yxmvmnod5g3p/v2 | https://www.protocols.io/view/flash-seq-low-amplification-protocol-b6f6rbre | Simone Picelli, Vincent Hahaut | TITLE: FLASH-seq Low-Amplification protocol
AUTHORS: Simone Picelli, Vincent Hahaut
[DESCRIPTION]
Building upon the existing Smart-seq2/3 workflows, we developed FLASH-seq (FS), a new full-length scRNA-seq method capable of detecting a significantly higher number of genes than both previous versions, requiring lim... | ["[Prepare lysis mix] Prepare the following lysis mix:\n ReagentReaction concentrationVolume (µl)384-well plateTriton-X100 (10% v/v)0.2%0.0208.448dNTP mix (25 mM each)6 mM0.240101.376SMART dT30VN (100 µM)1.8 mM0.0187.603RNAse inhibitor (40 U/µL)1.2 U/µL0.03012.672DTT (100 mM)1.2 mM0.0125.069FS TSO (100 µM)9.2 µM0.09... |
62,111 | Pre-extraction sample processing for CALIBER project | 4 | dx.doi.org/10.17504/protocols.io.n92ldz7xov5b/v1 | https://www.protocols.io/view/pre-extraction-sample-processing-for-caliber-proje-b8v7rw9n | James JN Kitson | TITLE: Pre-extraction sample processing for CALIBER project
AUTHORS: James JN Kitson
[DESCRIPTION]
This is a document that outlines the steps needed to prepare field-collected samples during the CALIBER project for archiving and further processing.
[STEPS]
SECTION: Setup
1. Source steel beads (ball bearings) for tiss... | ["[Setup] Source steel beads (ball bearings) for tissue grinding (Tungsten beads are not usually necessary). We use hardened carbon steel or stainless steel bearings from simplybearings.co.uk. This protocol requires two beads per sample tube.", "[Setup] Beads are usually shipped coated in manufacturing oil (especially ... |
69,778 | Astrocyte extraction from brain organoids | 4 | dx.doi.org/10.17504/protocols.io.261ge364wl47/v1 | https://www.protocols.io/view/astrocyte-extraction-from-brain-organoids-cgdsts6e | gustavo.parfitt | TITLE: Astrocyte extraction from brain organoids
AUTHORS: gustavo.parfitt
[DESCRIPTION]
Protocol for astrocyte extraction from brain organoids.
[STEPS]
2. Collect 10-20 spheres and place in a 6 well plate (day 40+ spheres)
1. Coat a 6 well plate coat with gelatin 0.1% or 1:100
3. Wash in PBS twice
4. Aspirat... | ["Collect 10-20 spheres and place in a 6 well plate (day 40+ spheres)", "Coat a 6 well plate coat with gelatin 0.1% or 1:100", "Wash in PBS twice", "Aspirate the PBS", "Add 1 mL of for 5 min", "Triturate using glass a pipette (2-3 up and down)", "Transfer the cells and tissue (even the chunks, the trituration is... |
null | null | null | dx.doi.org/10.17504/protocols.io.cjkukv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the Double Digest Protocol with Standard Restriction Enzymes, in the same buffer, using two separate incubation temperatures for the enzymes.
[BEFORE_START]
NEB's online tools, <a href="https://www.neb.com/tools-and-resources/interactive-tools/double-digest-fi... | [] |
63,908 | Via Keto Gummies Reviews | Is This Fat Burning Method To Effective? | 3 | dx.doi.org/10.17504/protocols.io.kxygxz2jdv8j/v1 | https://www.protocols.io/view/via-keto-gummies-reviews-is-this-fat-burning-metho-cancsdaw | Via Keto Gummies Reviews | TITLE: Via Keto Gummies Reviews | Is This Fat Burning Method To Effective?
AUTHORS: Via Keto Gummies Reviews
[DESCRIPTION]
https://www.eastbaytimes.com/2022/05/27/viaketo-apple-gummies-reviews/
[STEPS] | [] |
87,112 | Test_PDF_uploadV3 | 1 | dx.doi.org/10.17504/protocols.io.e6nvwddwzlmk/v3 | https://www.protocols.io/view/test-pdf-uploadv3-czbgx2jw | Brian, Brian Lee | TITLE: Test_PDF_uploadV3
AUTHORS: Brian, Brian Lee
[DESCRIPTION]
This is the another update to a fake NGN2 protocol is an example of just a pdf upload of a process. This is trying share things are still in development, or another version is now existing.
[STEPS] | [] |
70,544 | Chairside vs Labside All-Ceramic FDPs - A Systematic Review | 1 | dx.doi.org/10.17504/protocols.io.kqdg3953qg25/v1 | https://www.protocols.io/view/chairside-vs-labside-all-ceramic-fdps-a-systematic-cg5qty5w | tim.joda | TITLE: Chairside vs Labside All-Ceramic FDPs - A Systematic Review
AUTHORS: tim.joda
[DESCRIPTION]
Nowadays, the term digital is ubiquitous and everything has to be digitally branded to be up to date. Digitalization is not a trend, it is reality – and dental medicine is no exception. Dentistry is digital, the attribu... | ["SYSTEMATIC REVIEW\nChairside versus labside CAD/CAM monolithic all-ceramic fixed dental prostheses (FDPs): A systematic review", "Focused question (PICO)Do chairside versus labside CAD/CAM monolithic all-ceramic fixed dental prostheses demonstrate comparable outcomes in terms of cost-effectiveness, patient-reported o... |
48,106 | Protocols for "Chromosome-level genome assembly of the humpback puffer, Tetraodon palembangensis" | 2 | dx.doi.org/10.17504/protocols.io.bs8inhue | https://www.protocols.io/view/protocols-for-34-chromosome-level-genome-assembly-bs8inhue | Rui Zhang, Chang Li, Mengjun Yu, Xiaoyun Huang, Mengqi Zhang, Shanshan Liu, Shanshan Pan, Weizhen Xue, Congyan Wang, Chunyan Mao, He Zhang, Guangyi Fan | TITLE: Protocols for "Chromosome-level genome assembly of the humpback puffer, Tetraodon palembangensis"
AUTHORS: Rui Zhang, Chang Li, Mengjun Yu, Xiaoyun Huang, Mengqi Zhang, Shanshan Liu, Shanshan Pan, Weizhen Xue, Congyan Wang, Chunyan Mao, He Zhang, Guangyi Fan
[DESCRIPTION]
<div class = "text-blocks"><div class =... | [] |
90,384 | Cryoprotection of Mouse Brain | 1 | dx.doi.org/10.17504/protocols.io.yxmvm358ol3p/v1 | https://www.protocols.io/view/cryoprotection-of-mouse-brain-c4hqyt5w | Asta Zane, Nicole J Corbin-Stein, Gabrielle Childers, Jhodi Webster, Vickie Yang, Woong-Jai Won, Rajesh Gupta, Ashley Harms | TITLE: Cryoprotection of Mouse Brain
AUTHORS: Asta Zane, Nicole J Corbin-Stein, Gabrielle Childers, Jhodi Webster, Vickie Yang, Woong-Jai Won, Rajesh Gupta, Ashley Harms
[DESCRIPTION]
This protocol allows for accurate cryoprotection of mouse brain, post-perfusions, to be used for histology. The methods utilize another... | ["[PROCEDURE] Transfer the brain into 5-10ml of 4% PFA solution in PBS for 2 hours at room temperature in a 15 ml conical tube.", "[PROCEDURE] Transfer the brain into 30% sucrose solution in PBS, wait until it sinks to the bottom for 48-72 hours at 4°C.", "[PROCEDURE] Freeze brain on dry ice and store at minus -80 °C t... |
null | null | null | dx.doi.org/10.17504/protocols.io.c78zrv | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
To suppress both positive and negative phototactic behavior, we use an aversive stimulus. We place a filter paper wetted with either a 0.15M quinine/water solution (gustatory stimulus) or an 0.1 mixture (v/v) of benzaldehyde/paraffin oil (olfactory stimulus) in the preferred tube... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.jcncive | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Deep learning is an emerging field that promises unparalleled results on many data analysis problems. We show the success offered by such techniques when applied to the challenging problem of image-based plant phenotyping, and demonstrate state-of-the-art results for root and... | [] |
61,956 | Titan XL Male Enhancement Shred Muscles, Boost Sex Drive, Improve Energy Naturally And More(Work Or Hoax) | 3 | dx.doi.org/10.17504/protocols.io.kxygxz51ov8j/v1 | https://www.protocols.io/view/titan-xl-male-enhancement-shred-muscles-boost-sex-b8rcrv2w | Titan XL Male Enhancement | TITLE: Titan XL Male Enhancement Shred Muscles, Boost Sex Drive, Improve Energy Naturally And More(Work Or Hoax)
AUTHORS: Titan XL Male Enhancement
[DESCRIPTION]
Read This: "More Information From Knowledgeable Expertise of Titan XL Male Enhancement"
[STEPS] | [] |
82,285 | Whole Mouse Brain Delipidation - Dichloromethane | 1 | dx.doi.org/10.17504/protocols.io.dm6gpj7n5gzp/v1 | https://www.protocols.io/view/whole-mouse-brain-delipidation-dichloromethane-cukmwuu6 | Holly Myers, daphne.toglia | TITLE: Whole Mouse Brain Delipidation - Dichloromethane
AUTHORS: Holly Myers, daphne.toglia
[DESCRIPTION]
This protocol describes the delipidation of a mouse brain specimen using a modified iDISCO protocol. The brain is dehydrated with methanol and then cleared of lipids with dichloromethane to prepare for refractive ... | ["[Day 1: Dehydrate the PFA-fixed mouse brain in a step gradient of Methanol] Prepare 10mL of the following Methanol concentrations: \n\n20% Methanol in Milli-Q Water\n40% Methanol in Milli-Q Water\n60% Methanol in Milli-Q Water\n80% Methanol in Milli-Q Water", "Remove the Methanol solution used in the previous step fr... |
87,693 | Seawater virome concentration with Vivaflow | 1 | dx.doi.org/10.17504/protocols.io.ewov1qdopgr2/v1 | https://www.protocols.io/view/seawater-virome-concentration-with-vivaflow-czvmx646 | Natascha Varona, Cynthia Silveira | TITLE: Seawater virome concentration with Vivaflow
AUTHORS: Natascha Varona, Cynthia Silveira
[DESCRIPTION]
Concentration of viral particles from seawater samples using a Vivaflow.
[GUIDELINES]
This protocol is written for one sample only, however, with additional pumpheads and vivaflows you can run up to 4 viromes ... | ["[Virome collection] Collect 500mL - 2L of seawater (depending on microbial density) in a sterile container.", "[Virome collection] Wipe down outside of all masterflex tubing with 70% ethanol.", "[Virome collection] Insert one end of the tubing into the sample and feed it through the Masterflex pump.", "[Virome collec... |
87,729 | hsqc-tocsy_metab.nan | 5 | dx.doi.org/10.17504/protocols.io.8epv5x2rng1b/v1 | https://www.protocols.io/view/hsqc-tocsy-metab-nan-czwrx7d6 | NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison | TITLE: hsqc-tocsy_metab.nan
AUTHORS: NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison
[DESCRIPTION]
This is a protocol for running the Bruker pulse program "hsqcdietgpsisp.2".
[BEFORE_START]
This protocol assumes:
Your sample is loaded, locked, t... | ["[Create a new dataset]", "[Create a new dataset] On the menu bar on TopSpin, click on\nStart → Create Dataset", "[Create noesypr1d experiment file] When Lock, Tune, Shim are complete proceed to the specific protocol for your desired pulseprogram(s).", "[Acquire a spectrum]", "[Acquire a spectrum] Click on\nAcquire → ... |
86,418 | Collection and Analysis of Mouse Open Field Activity | 4 | null | https://www.protocols.io/view/collection-and-analysis-of-mouse-open-field-activi-cymsxu6e | Victoria Vance, Katerina Rademacher, Ken Nakamura | TITLE: Collection and Analysis of Mouse Open Field Activity
AUTHORS: Victoria Vance, Katerina Rademacher, Ken Nakamura
[DESCRIPTION]
The open field task is used to measure locomotor activity in mice. This protocol describes the setup of the task and the acquisition of open field data. It also covers the use of EthoVi... | ["[Setup] 1 hour prior to recording video, bring mice to behavior room to habituate.", "[Setup] Take two racks and place the clear acrylic sheet between them.", "[Setup] Clip the acrylic to the racks using large binder clips.", "[Setup] Tape 4 sheets of white paper together and wrap tightly around the outside of the cy... |
61,931 | Peptide C-Terminal Modification | 6 | dx.doi.org/10.17504/protocols.io.q26g743d3gwz/v1 | https://www.protocols.io/view/peptide-c-terminal-modification-b8qjrvun | Cathy Miller | TITLE: Peptide C-Terminal Modification
AUTHORS: Cathy Miller
[DESCRIPTION]
The post-translation modification of eukaryotic proteins by the addition of isoprenyl lipids at their C-termini was first observed in the 1970s and 1980s. Since then, more than a hundred proteins have been shown to be modified by C15 farnesyl ... | [] |
55,920 | Multicolour flow cytometry protocol for dogs | 1 | dx.doi.org/10.17504/protocols.io.kqdg3p45el25/v1 | https://www.protocols.io/view/multicolour-flow-cytometry-protocol-for-dogs-b2uqqevw | Takanori Kitamura, Maciej Parys | TITLE: Multicolour flow cytometry protocol for dogs
AUTHORS: Takanori Kitamura, Maciej Parys
[DESCRIPTION]
Although immunotherapy is becoming a standard approach of human cancer treatment, only a minor fraction of patients responds to the therapy. It is therefore required to determine the sub-populations of patients w... | ["[Sample collection and processing- blood and lymph node] Blood: Collect 1-2 mL of peripheral blood from the vein of dogs into tubes including EDTA.\nLN: Collect fine-needle aspirate, using a 23G needle from the enlarged LN and place it into EDTA tubes with RPMI 1640 supplemented with 10% FBS. \nThe yield and number o... |
null | null | null | dx.doi.org/10.17504/protocols.io.fwgbpbw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is for the JetSeqTM DNA Library Preparation Kit. The kit is designed to generate high-quality next generation sequencing (NGS) libraries suitable for sequencing on Illumina MiSeqTM, NextSeqTM or HiSeqTM instruments.</p>
[GUIDELINES]
<div class="page" title="Pag... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hhub36w | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
99,786 | Barcoded vector cloning | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1jj7lmk/v1 | https://www.protocols.io/view/barcoded-vector-cloning-ddpi25ke | Raining Wang, Melinda Wheelock | TITLE: Barcoded vector cloning
AUTHORS: Raining Wang, Melinda Wheelock
[DESCRIPTION]
This is the protocol for inserting barcodes to the N- and C- vector.
[BEFORE_START]
Ensure there are enough maxi-prep kits available to use.
Prepare 2-3 of 245 mm LB plus ampicillin/Carbenicillin agarose plates. Each plate should hav... | ["[Generate barcode amplicon] qPCR reaction: 25ul/rxn", "[Generate barcode amplicon] Purify with Zymo Clean & Concentrate with pooling 4 reactions together for one column: elute the column with 14 µL H2O. This is counting the volume needed for qubit and nanodrop.", "[Generate barcode amplicon] Thermocycling in a qPCR m... |
66,367 | Diatoxil Avis France : Avis d'expert sur ce produit ! {Prix & Détails} | 1 | dx.doi.org/10.17504/protocols.io.4r3l2oj7qv1y/v1 | https://www.protocols.io/view/diatoxil-avis-france-avis-d-39-expert-sur-ce-produ-cc27syhn | roxyrubo | TITLE: Diatoxil Avis France : Avis d'expert sur ce produit ! {Prix & Détails}
AUTHORS: roxyrubo
[DESCRIPTION]
Must Visit : https://www.facebook.com/DiaetoxilAvis/
https://ipsnews.net/business/2022/07/01/diaetoxil-avis-france-gelules-diaetoxil-erfahrungen-bezugsquellen-entgiftung-avis/
https://ipsnews.net/... | ["[Diatoxil Avis France] Diaetoxil Avis France : Le complément alimentaire révolutionnaire Diaetoxil Gélules, dont l’efficacité a été scientifiquement prouvée, est désormais disponible pour les personnes au régime qui souhaitent perdre du poids de la manière la plus naturelle possible tout en préservant leur santé et l... |
44,081 | NEBNext Single Cell/ Low Input RNA Library Prep Kit for Illumina E6420 Protocol for Cells | 4 | dx.doi.org/10.17504/protocols.io.81wgb762ovpk/v1 | https://www.protocols.io/view/nebnext-single-cell-low-input-rna-library-prep-kit-bparmid6 | New England Biolabs | TITLE: NEBNext Single Cell/ Low Input RNA Library Prep Kit for Illumina E6420 Protocol for Cells
AUTHORS: New England Biolabs
[DESCRIPTION]
The NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng ... | ["[Sample and Reagents Preparation] Briefly centrifuge the tubes containing NEBNext Single Cell RT Enzyme Mix and Murine RNase Inhibitor to collect solutions to the bottom of the tubes, then place on ice.", "[Sample and Reagents Preparation] Thaw all other frozen components at Room temperature (if the 10X NEBNext Cell ... |
63,075 | Immunohistochemistry | 4 | dx.doi.org/10.17504/protocols.io.14egn7nezv5d/v1 | https://www.protocols.io/view/immunohistochemistry-b9ubr6sn | Alexandra Nelson | TITLE: Immunohistochemistry
AUTHORS: Alexandra Nelson
[DESCRIPTION]
This protocol describes immunohistochemical staining of fixed brain sections.
[STEPS]
1. Sectioning
2. Blocking.
Incubate sections in blocking buffer at Room temperature for 1-2 hours.
Blocking buffer is: 3% NDS (normal donkey serum)/ 0.1% triton (a... | ["Sectioning", "Blocking. \nIncubate sections in blocking buffer at Room temperature for 1-2 hours.\nBlocking buffer is: 3% NDS (normal donkey serum)/ 0.1% triton (also called NDST) in PBS.", "Primary Antibody. \nPrepare primary antibody in 3% NDS at desired concentration (make sure this is the final concentration in t... |
91,816 | Protocol for Systematic Analysis Data Elements | 1 | dx.doi.org/10.17504/protocols.io.5qpvo3wodv4o/v1 | https://www.protocols.io/view/protocol-for-systematic-analysis-data-elements-c5wgy7bw | Anushka Sheoran, Maryann Martone, Abel Torres-Espin | TITLE: Protocol for Systematic Analysis Data Elements
AUTHORS: Anushka Sheoran, Maryann Martone, Abel Torres-Espin
[DESCRIPTION]
This protocol provides a detailed framework for the systematic annotation and analysis of the semantic interoperability of data elements in data shared through repositories. We applied this... | ["[SET UP] Document Organization: Download and systemically organize all required documents for curation, including datasets and data dictionaries (e.g., all data packages are stored in a folder called data set pool)", "[SET UP] Curation Record Keeping: Establish a DecisionLog/ READ-ME file to meticulously document al... |
null | null | null | dx.doi.org/10.17504/protocols.io.exbbfin | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Mix the components below for a total volume of <strong>20 µl.</strong>
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
78,526 | 1. CONSENT FORM | 1 | dx.doi.org/10.17504/protocols.io.rm7vzb9j4vx1/v1 | https://www.protocols.io/view/1-consent-form-cqw6vxhe | Haydeh Payami | TITLE: 1. CONSENT FORM
AUTHORS: Haydeh Payami
[DESCRIPTION]
This protocol details the consent form used for enrolling subjects into the study of interactions of gut microbiome, genetic susceptibility, and environmental factors in Parkinson's Disease, and collecting blood, saliva, stool, and metadata.
[STEPS] | [] |
68,269 | Preparation and Preservation of the Female Reproductive System (Ovaries, Fallopian Tubes, and Uterus) | 4 | dx.doi.org/10.17504/protocols.io.ewov1nr57gr2/v1 | https://www.protocols.io/view/preparation-and-preservation-of-the-female-reprodu-cewmtfc6 | Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill | TITLE: Preparation and Preservation of the Female Reproductive System (Ovaries, Fallopian Tubes, and Uterus)
AUTHORS: Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill
[DESCRIPTION]
This protocol describes procuremen... | ["[Modified aortic and portal cannulation strategy to perfuse pelvic organs] Conduct primary evaluation of ovaries, Fallopian tubes, and uterus to rule out structural abnormalities, malignancy, or infection.", "[Modified aortic and portal cannulation strategy to perfuse pelvic organs] Establish a cold perfusion line in... |
107,046 | Western Blot | 0 | null | https://www.protocols.io/view/western-blot-dkse4wbe | Karyna Tarasova, Angkana Kidtiwong, Sinan Gültekin, Florien Jenner | TITLE: Western Blot
AUTHORS: Karyna Tarasova, Angkana Kidtiwong, Sinan Gültekin, Florien Jenner
[DESCRIPTION]
Western Blot - protein expression assessment
[BEFORE_START]
Before start please check the antibody datasheet provided by the manufacturer.
[STEPS]
SECTION: Sample preparation
1. Protein Quantification - BCA... | ["[Sample preparation] Protein Quantification - BCA, Quibit measurment", "[Sample preparation] After determining how much protein to load, add an equal volume of 2X Laemmli sample buffer, in order to reduce and denature the samples, unless the online antibody datasheet indicates that non-reducing and non-denaturing con... |
17,456 | Modified Leeming and Notman Agar | 1 | dx.doi.org/10.17504/protocols.io.kxygxm3ywl8j/v1 | https://www.protocols.click/view/modified-leeming-and-notman-agar-vaqe2dw | Amy Gladfelter | TITLE: Modified Leeming and Notman Agar
AUTHORS: Amy Gladfelter
[DESCRIPTION]
Media recipe for growth of Malassezia species.
[STEPS]
1. bacteriological peptone (Oxoid) 10 g
2. yeast extract 2 g
3. desiccated ox bile (Oxoid) 8 g
4. glucose 10 g
5. glycerol monostearate 0.5 g
6. agar 15 g
7. glycerol, 50% 20 mL
... | ["bacteriological peptone (Oxoid) 10 g", "yeast extract 2 g", "desiccated ox bile (Oxoid) 8 g", "glucose 10 g", "glycerol monostearate 0.5 g", "agar 15 g", "glycerol, 50% 20 mL", "tween 60 5 mL", "olive oil 20 mL", "dH2O 955 mL", "Sterilize by autoclaving."] |
43,622 | Ethanol Quantification assay | 4 | dx.doi.org/10.17504/protocols.io.bnuemete | https://www.protocols.io/view/ethanol-quantification-assay-bnuemete | Marcos Valenzuela-Ortega | TITLE: Ethanol Quantification assay
AUTHORS: Marcos Valenzuela-Ortega
[DESCRIPTION]
Chemoenzymatic method for quantification of ethanol with a spectrophotometer at 500 nm (not UV !)
Based on Lewicka 2014 (DOI: 10.1021/sb500020g )
Adapted for analysis in plate reader of multiple samples at the same time.
[STEPS]
S... | ["[Prepare stocks] Prepare the following stocks using your tris buffer:\n\n \n Stock mg/mL mM Mw Yeast Adh 5 NAD+ 13.3 20 685.41 PMS 6.1 20 306.34 INTV 25.3 50 ... |
16,985 | Fast-Seq, a universal method for rapid and inexpensive genomic validation of rAAV vectors in preclinical settings | null | dx.doi.org/10.17504/protocols.io.utzewp6 | null | Lucy Maynard, Olivia Smith, Nicolas Tilmans, Eleonore Tham, Shayan Hosseinzadeh, Ryan Leenay, Weilun Tan, Nicole Paulk | TITLE: Fast-Seq, a universal method for rapid and inexpensive genomic validation of rAAV vectors in preclinical settings
AUTHORS: Lucy Maynard, Olivia Smith, Nicolas Tilmans, Eleonore Tham, Shayan Hosseinzadeh, Ryan Leenay, Weilun Tan, Nicole Paulk
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">San... | ["[SOLUTIONS TO PREPARE IN ADVANCE OF AAV LIBRARY PREP AND STORE]\nThe dilution of each new batch of adapter-loaded Tn5 enzyme should be empirically determined based on activity. We recommend making a dilution series of loaded enzyme to determine the optimal yield and fragment size. Dilute the Tn5 enzyme in Tn5 Storage... |
19,127 | Intrapancreatic injection surgery | 1 | dx.doi.org/10.17504/protocols.io.14egnx47pl5d/v1 | https://www.protocols.click/view/intrapancreatic-injection-surgery-wwxfffn | Maria Jimenez Gonzalez, Rosemary Li, Sarah Stanley | TITLE: Intrapancreatic injection surgery
AUTHORS: Maria Jimenez Gonzalez, Rosemary Li, Sarah Stanley
[DESCRIPTION]
This is a protocol for performing intrapancreatic injection of Cholera Toxin Beta (CTb) in mice.
[BEFORE_START]
-This study was approved by the Institutional Animal Care and Use Committee of Icahn Schoo... | ["[Preparing surgery area] - Prepare the surgical space by placing the absorbant pad and the inhalatory anesthesia. Write down the mouse weight. \n- Anesthetize the animal by inducing anesthesia with 3% Isofluorane. Once anesthesia is induced, place animal in supine position and keep anesthesia at 1-1.5%.\n- Apply eye ... |
96,846 | Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment | 4 | dx.doi.org/10.17504/protocols.io.36wgq3zmylk5/v2 | https://www.protocols.io/view/lipopolysaccharide-intraperitoneal-injection-in-ra-datn2eme | mariangela.massarocenere | TITLE: Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment
AUTHORS: mariangela.massarocenere
[DESCRIPTION]
This protocol is a quick guide to how to prepare a solution of LPS on the saline vehicle, how to inject a rat for an intraperitoneal injection, and how to monitor the animal aft... | ["[1. Lipopolysaccharide (LPS) preparation] Before starting, it is better to prepare LPS solution on the same day it will be injected. However, under minimal conditions (that could be acceptable), you can work with LPS from previous days stored at kept at 2-8°C; in this case, shake very well before use.", "[1. Lipopoly... |
47,746 | Protein A and Protein AG Sandwich ELISA | 1 | dx.doi.org/10.17504/protocols.io.bsvane2e | https://www.protocols.io/view/protein-a-and-protein-ag-sandwich-elisa-bsvane2e | Angel Justiz-Vaillant | TITLE: Protein A and Protein AG Sandwich ELISA
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-AG (SpAG) with different immunoglobulin preparations from mammalian and avia... | ["This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-AG (SpAG) with different immunoglobulin preparations from mammalian and avian species. The 96 well microtiter plate was coated overnight at 4°C with 2 µg/µl per well of SpA in carbonate-bicarbonate buffer pH 9.6.", "The p... |
13,502 | Phenol-chloroform DNA purification | 1 | dx.doi.org/10.17504/protocols.io.re6d3he | https://www.protocols.io/view/phenol-chloroform-dna-purification-re6d3he | Tomasz Suchan | TITLE: Phenol-chloroform DNA purification
AUTHORS: Tomasz Suchan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Because of the influence of salts on enzymatic reactions, Qiagen extractions can be purified before making RAD tags. First, it is necessary to perform a chloropane extraction (phenol / ch... | ["[Phenol-chloroform extraction]\nTo 100ul eluted DNA, add 0.5 ul of 20% SDS (*this may not be necessary as there are no cells) and 100 ul of phenol-chloroform.", "[Phenol-chloroform extraction]\nVortex well.", "[Phenol-chloroform extraction]\nCentrifuge at room temperature for 5 min, at full speed (14,000 rpm).", "[Ph... |
null | null | null | dx.doi.org/10.17504/protocols.io.iv7ce9n | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
33,282 | Tissue Preparation for Spatial Metabolomics | null | dx.doi.org/10.17504/protocols.io.bcraiv2e | null | Guanshi Zhang, Dusan Velickovic, Annapurna Pamreddy, Jessica Lukowski, Theodore Alexandrov, Chris Anderton, Kumar Sharma | TITLE: Tissue Preparation for Spatial Metabolomics
AUTHORS: Guanshi Zhang, Dusan Velickovic, Annapurna Pamreddy, Jessica Lukowski, Theodore Alexandrov, Chris Anderton, Kumar Sharma
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Mass spectrometry imaging (MSI) is a cutting-edge molecular technology ... | ["[Snap frozen (liquid N2) sample preparation and sectioning]\nRemove fresh frozen (liquid nitrogen) kidney sample stored at and place in cryostat set at\n-80 °C\n-15 °C", "[Snap frozen (liquid N2) sample preparation and sectioning]\nMount the sample on chuck with minimal amount of water (one droplet) and make sect... |
58,941 | Immunofluorescent Staining | 4 | dx.doi.org/10.17504/protocols.io.b5s5q6g6 | https://www.protocols.io/view/immunofluorescent-staining-b5s5q6g6 | Haley Geertsma | TITLE: Immunofluorescent Staining
AUTHORS: Haley Geertsma
[DESCRIPTION]
This protocol is used to stain cryosectioned mouse brain tissue.
[STEPS]
1. To cryo-sectioned brain tissue, wash with 1X phosphate buffered saline (PBS) for 3x 5-minute washes.
2. Incubate in blocking buffer for 1 hour at room temperature.
Bloc... | ["To cryo-sectioned brain tissue, wash with 1X phosphate buffered saline (PBS) for 3x 5-minute washes.", "Incubate in blocking buffer for 1 hour at room temperature.\nBlocking buffer: 10% serum + 0.5% Triton X-100 in 1X PBS", "Wash tissue with 1X PBS.", "Incubate in primary antibody diluted in blocking buffer overnight... |
23,623 | EpiTect Plus Bisulfite Conversion | null | dx.doi.org/10.17504/protocols.io.3bfgijn | null | Bing Yang | TITLE: EpiTect Plus Bisulfite Conversion
AUTHORS: Bing Yang
[STEPS] | [] |
76,696 | Terrific broth (TB) medium | 4 | null | https://www.protocols.io/view/terrific-broth-tb-medium-cn5yvg7w | Andreas Sagen | TITLE: Terrific broth (TB) medium
AUTHORS: Andreas Sagen
[DESCRIPTION]
IBI’s Terrific Broth is used with Glycerol in cultivating recombinant strains of E. coli. Terrific broth is a highly enriched medium for improving yield in plasmid bearing E. coli. Recombinant strains have an extended growth phase in the medium. Th... | ["[Terrific broth base solution] Fill the bottle with 200 mL distilled water", "[Terrific broth base solution] Measure 11.8 g Yeast extract and 5.9 g Tryptone. Add 7.5 g for making agar plates\n\nMaterials:", "[Terrific broth base solution] Add powdered solids into bottle, and use a magnetic mixer with a stir bar to mi... |
62,271 | Ikaria Lean Belly Juice Uk Reviews | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn93qvx9/v1 | https://www.protocols.io/view/ikaria-lean-belly-juice-uk-reviews-b827ryhn | lkarialeanbellyreviews | TITLE: Ikaria Lean Belly Juice Uk Reviews
AUTHORS: lkarialeanbellyreviews
[DESCRIPTION]
Ikaria Lean Belly Juice Uk Reviews
[STEPS]
1. Ikaria Spare Belly Juice is an advanced superfood mix, offering easy and natural weight loss, especially for people with stubborn belly fat. It's made with clinically proven constit... | ["Ikaria Spare Belly Juice is an advanced superfood mix, offering easy and natural weight loss, especially for people with stubborn belly fat. It's made with clinically proven constituents with maximum support in fat burning by controlling uric acid, fat oxidation, appetite, inflammation and other threat factors of rot... |
84,566 | Mixed-methods study to validate and refine the 'Strategic Healthcare Implementation Framework for Task Shifting, Sharing and Resource Enhancement' (SHIFT-SHARE) | 1 | dx.doi.org/10.17504/protocols.io.36wgq35zylk5/v1 | https://www.protocols.click/view/mixed-methods-study-to-validate-and-refine-the-39-cwtwxepe | Shukanto Das, Liz Grant, David Weller | TITLE: Mixed-methods study to validate and refine the 'Strategic Healthcare Implementation Framework for Task Shifting, Sharing and Resource Enhancement' (SHIFT-SHARE)
AUTHORS: Shukanto Das, Liz Grant, David Weller
[DESCRIPTION]
Background: In healthcare, task shifting and task sharing (TS/S) are mechanisms th... | ["[Seeking of consent] The consent process will ensure that participants have clear understanding of the study and voluntarily provide consent to participate. Once participants are identified, the Investigator will send them a participation information sheet (PIS), which will contain explanation of the study's purpose,... |
44,330 | RT-free Nanopore direct RNA sequencing v1 | 1 | dx.doi.org/10.17504/protocols.io.bpiimkce | https://www.protocols.io/view/rt-free-nanopore-direct-rna-sequencing-v1-bpiimkce | Miten Jain | TITLE: RT-free Nanopore direct RNA sequencing v1
AUTHORS: Miten Jain
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Reverse Transcription-free (RT-free) poly(A) RNA protocol for Nanopore Direct RNA Sequencing</span></div><div class = "text-block">This protocol was ... | ["Preparing input RNA:Record the quality, quantity and size of the input RNA.Guide criteria: Average fragment size: > 500 basesMass by Qubit RNA HS assay: Please ensure there are no detergents or surfactants in the bufferMass by Qubit RNA HS assay: Transfer of RNA to a PCR tube.Adjust the volume to with nuclease-f... |
null | null | null | dx.doi.org/10.17504/protocols.io.j9kcr4w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>RNA samples (RNA concentration: 100 ng/mL) were extracted from the kidney tissues of both homozygous knockout and wild-type mice and subjected to gene array analyses (Agilent Technologies, Santa Clara, CA) (GEO array GES57590). RNA samples were extracted from the blood of hea... | [] |
97,064 | Anti-GFP pull-down with syringe filter partition | 0 | dx.doi.org/10.17504/protocols.io.5qpvokx79l4o/v1 | https://www.protocols.io/view/anti-gfp-pull-down-with-syringe-filter-partition-da2g2gbw | Dorothy zhao | TITLE: Anti-GFP pull-down with syringe filter partition
AUTHORS: Dorothy zhao
[DESCRIPTION]
Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's disease (HD). Huntingtin (Htt), the ... | ["[Sample lysis and filter partition] Htt64Q-GFP induced with Muristerone A for polyQ expression in Neuro2a, for two days in a 15cm dish", "[Sample lysis and filter partition] wash with PBS, and lysed in cold isotonic buffer (0.25M sucrose, 1mM EDTA, 20mM HEPES pH 7.4) with protease inhibitor, with a gauge 22-needle fo... |
97,946 | Morphometry of thyroid cartilage, epiglottis and pyriform fossa | 0 | dx.doi.org/10.17504/protocols.io.x54v92m8ml3e/v1 | https://www.protocols.io/view/morphometry-of-thyroid-cartilage-epiglottis-and-py-dbv22n8e | Murlimanju Virupakshamurthy | TITLE: Morphometry of thyroid cartilage, epiglottis and pyriform fossa
AUTHORS: Murlimanju Virupakshamurthy
[DESCRIPTION]
Larynx is a complex organ of voice production and respiration, which is supported by a series of cartilages, membranes, muscles and joints. They bring about movement of vocal cords with a considera... | ["[Morphometry of thyroid cartilage, epiglottis and pyriform fossa] The goal of this study will be to obtain\nthe dimensions of thyroid cartilage, epiglottis and piriform sinus in embalmed\ncadavers of Indian sample population."] |
63,335 | ketp complete | 1 | dx.doi.org/10.17504/protocols.io.n92ldzq4ov5b/v1 | https://www.protocols.io/view/ketp-complete-b94fr8tn | keto complete | TITLE: ketp complete
AUTHORS: keto complete
[DESCRIPTION]
https://wintersupplement.com/keto-complete-australia/
Keto Complete Australia
With regards to Keto Complete Australia, it's basic to comprehend that a keto or 'ketogenic' plan is connected to a high-fat, low-carb food that helps weight decrease and advances fur... | [] |
101,666 | Single nuclei RNA sequencing (snRNA-seq) of frozen human lung tissue and hPCLS | 0 | dx.doi.org/10.17504/protocols.io.36wgqndb5gk5/v1 | https://www.protocols.io/view/single-nuclei-rna-sequencing-snrna-seq-of-frozen-h-dfia3kae | Heidi Monroe, Nayra Cardenes, Oliver Eickelberg, Melanie Königshoff, koenigshoffm, Robert Lafyatis | TITLE: Single nuclei RNA sequencing (snRNA-seq) of frozen human lung tissue and hPCLS
AUTHORS: Heidi Monroe, Nayra Cardenes, Oliver Eickelberg, Melanie Königshoff, koenigshoffm, Robert Lafyatis
[DESCRIPTION]
Single-cell RNA sequencing (scRNA-seq) has become an essential tool for delineating cellular diversity in norma... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hk4b4yw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Colony PCR using DreamTaq Green PCR mastermix (based on manufacturer ThermoFisher Scientific protocol).</p>
[STEPS]
?.
?. | [] |
58,286 | Rapid Run v2 Primer Rehyb | 1 | dx.doi.org/10.17504/protocols.io.b46nqzde | https://www.protocols.io/view/rapid-run-v2-primer-rehyb-b46nqzde | Amanda Chilaka, Sumeet Gupta | TITLE: Rapid Run v2 Primer Rehyb
AUTHORS: Amanda Chilaka, Sumeet Gupta
[DESCRIPTION]
Switch the flowcell to another sequencer if first base fails - HiSeq
[GUIDELINES]
If a rapid run has failed at first base and you want to switch the flowcell to another side or sequencer, follow these steps (ONLY applicable to sing... | ["[Rapid Run v2 Primer Rehyb] Wash the sequencer that will be used (MWB and then water). [Link to wash protocol]", "[Rapid Run v2 Primer Rehyb] Load the SBS rack (with reagents) to the sequencer.", "[Rapid Run v2 Primer Rehyb] Load water in the Paired End rack and the template tubes.", "[Rapid Run v2 Primer Rehyb] Plac... |
44,319 | Reverse Transcribe RNA (All Clip and Input Samples), and Reaction Cleanup | 4 | dx.doi.org/10.17504/protocols.io.bph7mj9n | https://www.protocols.io/view/reverse-transcribe-rna-all-clip-and-input-samples-bph7mj9n | Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo | TITLE: Reverse Transcribe RNA (All Clip and Input Samples), and Reaction Cleanup
AUTHORS: Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Profiling of RNA binding... | ["[Anneal Primer in 8-Well Strip Tubes]\nMix with .\n[RNA]\n[InvAR17 primer]", "[Anneal Primer in 8-Well Strip Tubes]\nHeat at for in preheated PCR block, place immediately .\n65 °C\non ice", "[Prepare Reverse Transcription Master Mix on Ice; 10 μL Per Sample]\nAB1H2O4.0 μL210× AffinityScript Buffer2.0 μL30.1 M DTT2... |
45,517 | Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer | 4 | dx.doi.org/10.17504/protocols.io.bqpmmvk6 | https://www.protocols.io/view/quantitative-real-time-measurements-of-intracellul-bqpmmvk6 | Matthew B. Robers, James D. Vasta, Cesear R. Corona, Rachel Friedman Ohana, Robin Hurst, Manisha A. Jhala, Kenneth M. Comess, Keith V. Wood | TITLE: Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer
AUTHORS: Matthew B. Robers, James D. Vasta, Cesear R. Corona, Rachel Friedman Ohana, Robin Hurst, Manisha A. Jhala, Kenneth M. Comess, Keith V. Wood
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In... | ["[3.2.1 [Day 1]: Transient Transfection of HEK293 Cells with NanoLuc(R) Fusions]\nCultivate desired cells (e.g., HEK293) appropriately prior to assay and resuspend cells into a single-cell suspension using complete cell culture medium.", "[3.2.1 [Day 1]: Transient Transfection of HEK293 Cells with NanoLuc(R) Fusions]\... |
60,073 | Intracellular metabolomics extraction | 1 | dx.doi.org/10.17504/protocols.io.j8nlkk985l5r/v1 | https://www.protocols.io/view/intracellular-metabolomics-extraction-b6whrfb6 | Juan Sanchez | TITLE: Intracellular metabolomics extraction
AUTHORS: Juan Sanchez
[DESCRIPTION]
This quench and intracellular extraction are optimal for the analysis of metabolites stable at high temperatures and moderately acidic pH that are rapidly consumed such as pyruvate and acetyl-CoA. It also avoids the use of methanol which... | ["[Fast Filtration] Harvest cells.\nThe recommended biomass input for mammalian cells is 10 million cells. For bacteria and yeast, 6 relative ODs are recommended.", "[Fast Filtration] Dispense cells onto a 47 mm nylon filter with a 0.2 µm pore size under vacuum in a vacuum filtration assembly. If desired the supernatan... |
88,042 | 18S V9 PCR | 4 | null | https://www.protocols.io/view/18s-v9-pcr-cz8ix9ue | Kathleen Pitz | TITLE: 18S V9 PCR
AUTHORS: Kathleen Pitz
[DESCRIPTION]
This protocol is aimed at amplifying the 18S rRNA hypervariable region 9 (18S V9) in eukaryotes with a focus on microbial eukaryotes. Amplicons generated using this protocol can then be sequenced using the Illumina platform. The primers (1391F, EukBr) utilized in ... | ["[Minimum Information about an Omics Protocol (MIOP)] MIOP Term\nValue\n \nmethodology category\n\n \nproject\n\n \npurpose\n\n \nanalyses\n\n \ngeographic location\n\n \nbroad-scale environmental context\n\n \nlocal environmental context\n\n \nenvironmental medium\n\n \ntarget\n18S gene\n \ncreator\n\n \nmaterials ... |
60,113 | Collecting samples for Dissolved Organic Carbon (DOC) analysis | 1 | null | https://www.protocols.io/view/collecting-samples-for-dissolved-organic-carbon-do-b6xrrfm6 | Krista Longnecker | TITLE: Collecting samples for Dissolved Organic Carbon (DOC) analysis
AUTHORS: Krista Longnecker
[DESCRIPTION]
This protocol describes collecting samples for Dissolved Organic Carbon (DOC) analysis. Dissolved organic carbon is operationally defined as the material that passes through a filter with a known pore size. ... | ["[Collecting samples for Total Organic Carbon (TOC) analysis] The glass vials need to be combusted before use.", "[Collecting samples for Total Organic Carbon (TOC) analysis] Set aside the caps that were sent with the vials because they cannot be combusted.", "[Collecting samples for Total Organic Carbon (TOC) analysi... |
null | null | null | dx.doi.org/10.17504/protocols.io.jr2cm8e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The steps that species richness were calculated in each grid cell within DIVA-GIS Version 5.2,and endemicity analysis (EA) performed by NDM to identify areas of endemism.</p>
[STEPS] | [] |
91,374 | Detection of accessible cholesterol in primary cilia using purified His-ALOD4-mNeon in 3T3 Fibroblasts | 4 | dx.doi.org/10.17504/protocols.io.rm7vzx2qxgx1/v1 | https://www.protocols.io/view/detection-of-accessible-cholesterol-in-primary-cil-c5gny3ve | Sreeja V Nair, Ayan Adhikari, Suzanne R Pfeffer | TITLE: Detection of accessible cholesterol in primary cilia using purified His-ALOD4-mNeon in 3T3 Fibroblasts
AUTHORS: Sreeja V Nair, Ayan Adhikari, Suzanne R Pfeffer
[DESCRIPTION]
There exist at least three different pools of cholesterol in the plasma membrane: the essential pool, the sphingomyelin-sequestered pool, ... | ["[His-mNeon ALOD4 purification] Make a 50 mL starter culture of BL21 DE3 Rosetta plysS cells expressing pRSET+ His-mNeon-FLAG-ALOD4, starting from a fresh colony picked from freshly transformed plates containing carbenicillin (100μg/ml) and chloramphenicol (34μg/ml). Grow with shaking in an Erlenmeyer flask at 37 °C... |
86,860 | Sanger Tree of Life RNA Extraction: Manual TRIzol™ | 4 | dx.doi.org/10.17504/protocols.io.yxmvm334nl3p/v1 | https://www.protocols.io/view/sanger-tree-of-life-rna-extraction-manual-trizol-cy3kxykw | Raquel Juliana Vionette do Amaral, Clare Cornwell, Caroline Howard | TITLE: Sanger Tree of Life RNA Extraction: Manual TRIzol™
AUTHORS: Raquel Juliana Vionette do Amaral, Clare Cornwell, Caroline Howard
[DESCRIPTION]
This protocol describes the manual extraction of RNA from multiple different tissue samples intended for RNA-Seq, using the TRIzol™ Reagent, based on the Thermo Fisher TRI... | ["[Sample lysis] Place the samples on dry ice.", "[Sample lysis] For cryoprepped samples: \na) Label a 1.5 mL microcentrifuge tube for each sample.\nb) Add 500 μL of TRIzol into each 1.5 mL microcentrifuge tube.\nc) Add 500 μl of TRIzol into the tube containing the sample. Gently mix with a wide-bore tip and transfer t... |
64,286 | Protocol for Systematic review based on PROSPERO guidelines (adapted) | 3 | null | https://www.protocols.io/view/protocol-for-systematic-review-based-on-prospero-g-caz6sf9e | Mathilda Featherston-Lardeux | TITLE: Protocol for Systematic review based on PROSPERO guidelines (adapted)
AUTHORS: Mathilda Featherston-Lardeux
[DESCRIPTION]
This protocol sets out the approach taken for the systematic review: the causal effect of the Chinese pilot emission trading scheme on CO2 emissions.
[STEPS] | [] |
48,625 | Antioxidant activity by FRAP assay: in vitro protocol | 6 | dx.doi.org/10.17504/protocols.io.btqrnmv6 | https://www.protocols.io/view/antioxidant-activity-by-frap-assay-in-vitro-protoc-btqrnmv6 | Adrieli Sachett, Matheus Gallas-Lopes, Greicy M M Conterato, Ana Herrmann, Angelo Piato | TITLE: Antioxidant activity by FRAP assay: in vitro protocol
AUTHORS: Adrieli Sachett, Matheus Gallas-Lopes, Greicy M M Conterato, Ana Herrmann, Angelo Piato
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Considering the role of oxidative stress in the pathology of several diseases and the us... | ["[Preparing the reagents]\nThe first step is to prepare the reagents to be used in this protocol;", "[Preparing the reagents]\nAcetate buffer : 1.1.1 Weigh of sodium acetate in a beaker of appropriate size; 1.1.2 Dissolve the salt with of ultrapure water; 1.1.3 Transfer the solution to a volumetric flask;... |
35,410 | Extracellular DNA extraction from sediment using phosphate buffer and NucleoSpin® Soil kit (MACHEREY NAGEL) | 1 | dx.doi.org/10.17504/protocols.io.betsjene | https://www.protocols.io/view/extracellular-dna-extraction-from-sediment-using-p-betsjene | Cecile Chardon, Louis Jacas, Isabelle Domaizon | TITLE: Extracellular DNA extraction from sediment using phosphate buffer and NucleoSpin® Soil kit (MACHEREY NAGEL)
AUTHORS: Cecile Chardon, Louis Jacas, Isabelle Domaizon
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div></div>
[STEPS]
?. [... | ["[Prepare the sample]\nAnnotate 2mL tubes, one 2mL tube per sampleWeigh annotated tubes, use the weigh scale (0.0001g of precision) → On your notebook, note the name of tubes and their weightsWith a sterilized spatula, homogenize a sediment and check that it is completely thawed (if not, wait for total defrost... |
37,630 | Calibration Protocol - Plate Reader Abs600 (OD) Calibration with Microsphere Particles | null | dx.doi.org/10.17504/protocols.io.bgy6jxze | https://www.protocols.io/view/calibration-protocol-plate-reader-abs600-od-calibr-bgy6jxze | Jacob Beal, Traci Haddock-Angelli, Markus Gershater, Vishal Sanchania, Russell Buckley-Taylor, Geoff Baldwin, Natalie Farny, Richard Tennant, Paul Rutten | TITLE: Calibration Protocol - Plate Reader Abs600 (OD) Calibration with Microsphere Particles
AUTHORS: Jacob Beal, Traci Haddock-Angelli, Markus Gershater, Vishal Sanchania, Russell Buckley-Taylor, Geoff Baldwin, Natalie Farny, Richard Tennant, Paul Rutten
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-bl... | ["[Prepare the Microsphere Stock Solution]\nObtain the tube labeled “Silica Beads” from the Measurement Kit and vortex vigorously for 30 seconds.\nMicrospheres should NOT be stored at 0°C or below, as freezing affects the properties of the microspheres. If you believe your microspheres may have been frozen, please cont... |
41,295 | Mmolecular COVID-19 Extraction-Free Direct-One-Step Fast Cycling Protocol | 4 | dx.doi.org/10.17504/protocols.io.bkjpkumn | https://www.protocols.io/view/mmolecular-covid-19-extraction-free-direct-one-ste-bkjpkumn | steve.puts | TITLE: Mmolecular COVID-19 Extraction-Free Direct-One-Step Fast Cycling Protocol
AUTHORS: steve.puts
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Direct One-Step RT-qPCR COVID-19 Test Kit is designed for quantitative</div><div class = "text-block">real-time analysis of target RNA directly fr... | ["[Sample processing]\nPlace the swab brush into a 1.5ml microcentrifuge tube containing and .Rotate the brush 5-10 times.Squeeze the brush and remove it from the tube.\n[PCR-grade water]\n[PBS (10x conc)]", "[Sample processing]\nCentrifuge at for 3 min at room temperature\nCentrifuge: 12000 34", "[Sample processing... |
78,378 | Spotlight highlights - discussing peer-review | 1 | dx.doi.org/10.17504/protocols.io.261ge3k67l47/v1 | https://www.protocols.io/view/spotlight-highlights-discussing-peer-review-cqsivwce | Gabriel Gasque | TITLE: Spotlight highlights - discussing peer-review
AUTHORS: Gabriel Gasque
[DESCRIPTION]
This protocol offers snipets of interviews with PLOS ONE Lab Protocol authors discussing the motivations to peer-review their protocols, the significance of the peer-reviewer feedback they got, and the implications that these a... | ["[Motivation for peer-reviewing protocols] Danelle Agnew - Macquarie University, AUSTRALIA", "[Motivation for peer-reviewing protocols] Jacopo Cerasoni- Loyola University, USA", "[Motivation for peer-reviewing protocols] Noah Langenfeld - Utah State University, USA", "[Motivation for peer-reviewing protocols] Simon Tr... |
null | null | null | dx.doi.org/10.17504/protocols.io.jcecite | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
92,437 | mouse brain storage and sectioning | 1 | dx.doi.org/10.17504/protocols.io.dm6gp3mo5vzp/v1 | https://www.protocols.io/view/mouse-brain-storage-and-sectioning-c6hvzb66 | Tony Hsiao | TITLE: mouse brain storage and sectioning
AUTHORS: Tony Hsiao
[DESCRIPTION]
detailed protocol to receive mouse brain tissue from US to Australia including storage and sectioning
[STEPS]
SECTION: Storage and sectioning of mouse brain tissue
1. Archive mice brains for storage
SECTION: Storage and sectioning of mouse... | ["[Storage and sectioning of mouse brain tissue] Archive mice brains for storage", "[Storage and sectioning of mouse brain tissue] Sample arrive in the delivery room (4°C)", "[Storage and sectioning of mouse brain tissue] Move samples from the delivery room to PC2 laboratory.", "[Storage and sectioning of mouse brain t... |
97,040 | Freezing Adherent Cell Lines | 0 | dx.doi.org/10.17504/protocols.io.5qpvokxq9l4o/v1 | https://www.protocols.io/view/freezing-adherent-cell-lines-dazq2f5w | Carolina Lopez | TITLE: Freezing Adherent Cell Lines
AUTHORS: Carolina Lopez
[DESCRIPTION]
This protocol describes how to freeze Adherent cells. Examples of these cells are: A549 cells, LLCMK2 cells, and MDCK cells.
[STEPS]
SECTION: Freezing Adherent cells
1.
Wash flask 2x with sterile PBS.
Add 2mL of trypsin/T75. Incubate at 37 oC ... | ["[Freezing Adherent cells] Wash flask 2x with sterile PBS.\nAdd 2mL of trypsin/T75. Incubate at 37 oC for 2-3 mins or until cells are detached from the flask.\nAdd 8mL of cell culture media and pipette up and down. Transfer all the media to a 15mL tube. \nCentrifuge at 1200 rpm for 5 min. \nDiscard supernatant and res... |
47,613 | Optimized Derivation of Midbrain Dopaminergic Neurons from iPSCs for research application | 1 | dx.doi.org/10.17504/protocols.io.bsq5ndy6 | https://www.protocols.io/view/optimized-derivation-of-midbrain-dopaminergic-neur-bsq5ndy6 | Elisangela Bressan, Ashutosh Dhingra, Stella Donato, Peter Heutink | TITLE: Optimized Derivation of Midbrain Dopaminergic Neurons from iPSCs for research application
AUTHORS: Elisangela Bressan, Ashutosh Dhingra, Stella Donato, Peter Heutink
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><span>The derivation of hum... | ["[Maintenance of iPSCs before start of differentiation]\nGrow iPSCs on Matrigel hESC-Qualified Matrix-coated 6-well plates in Essential 8 Flex (E8) medium for 4-5 days. Cells can be passaged once they reach 70-80% confluency (i.e., 70-80% of plate growth area is covered by a cell monolayer) with Gentle Dissociation Re... |
91,624 | CMV Resistance testing (UL54 and UL97) | 4 | dx.doi.org/10.17504/protocols.io.bp2l6xy4zlqe/v1 | https://www.protocols.io/view/cmv-resistance-testing-ul54-and-ul97-c5qgy5tw | Fernando Lazaro | TITLE: CMV Resistance testing (UL54 and UL97)
AUTHORS: Fernando Lazaro
[DESCRIPTION]
This protocol is a procedure for the study of antiviral resistance in Cytomegalovirus by NGS techniques.
The primers have been designed using https://primalscheme.com/ with the intention of covering the most relevant regions of the UL... | ["[Prepare Reagents] Q5‱ High-Fidelity DNA Polymerase (New England)\nAgarose gel 1%\nEthanol 70%\nMag-Bind‱ TotalPure NGS (omega)\nElution Buffer", "[DNA extraction] Perform DNA extraction with your method of choice. Preferably from a plasma sample collected on the same day.Perform DNA extraction with your method of ch... |
44,752 | PBMC- 02 - CD4+ T cell Isolation from PBMC with “Dynabeads CD4 Positive Isolation Kit” | 4 | dx.doi.org/10.17504/protocols.io.bpxqmpmw | https://www.protocols.io/view/pbmc-02-cd4-t-cell-isolation-from-pbmc-with-dynabe-bpxqmpmw | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | TITLE: PBMC- 02 - CD4+ T cell Isolation from PBMC with “Dynabeads CD4 Positive Isolation Kit”
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">List of published works using ... | ["Isolate PBMCs according either to the standard protocol from fresh blood or from buffy coat (PBMC- 01a - Isolation of Human PBMC from Buffy Coat, PBMC- 01b - Isolation of Human PBMC from Whole Blood).", "Count the cells with Cellometer machine or by manual count, using either Trypan Blue or Türk solutions accordingl... |
61,341 | PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX) | 4 | null | https://www.protocols.io/view/pcr-mixture-and-condition-2x-supergreen-pcr-master-b755rq86 | Yin-Tse Huang | TITLE: PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX)
AUTHORS: Yin-Tse Huang
[DESCRIPTION]
PCR mixture and condition (2X SUPERGREEN PCR MASTER MIX)
[STEPS]
SECTION: For generic primers
1. PCR mixture PCR condition for generic primers
SECTION: For generic primers
2. PCR condition for generic primers
... | ["[For generic primers] PCR mixture PCR condition for generic primers", "[For generic primers] PCR condition for generic primers", "[For barcoded primers] PCR mixture for barcoded primers", "[For barcoded primers] PCR condition for barcoded primers"] |
80,981 | IHC_cFOS+Parvalbumin | 4 | dx.doi.org/10.17504/protocols.io.81wgbyb9yvpk/v1 | https://www.protocols.io/view/ihc-cfos-parvalbumin-ctbvwin6 | Bilge Buyukdemirtas | TITLE: IHC_cFOS+Parvalbumin
AUTHORS: Bilge Buyukdemirtas
[DESCRIPTION]
Immunohistochemistry protocol for c-FOS and Parvalbumin co-staining of mouse brain tissue slices (30 microns thick).
[GUIDELINES]
All shaker incubations are done at 200 rpm speed.
[STEPS]
SECTION: Staining
1. Antigen Retrieval
Remove PBS from wel... | ["[Staining] Antigen Retrieval\nRemove PBS from wells. Add 500ul 0.3% citrate buffer into each well.", "[Staining] Incubate at RT on shaker for 30 min\nIncubate at 65 °C for 45 min\nCool down on the bench for 20 min", "[Staining] 3X PBS Washes", "[Staining] Remove citrate buffer from wells and replace with 500ul 1X PBS... |
null | null | null | dx.doi.org/10.17504/protocols.io.dna5ad | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
58,326 | Enrichment of motile phototrophs by phototaxis | 4 | dx.doi.org/10.17504/protocols.io.b47wqzpe | https://www.protocols.io/view/enrichment-of-motile-phototrophs-by-phototaxis-b47wqzpe | Usha Lingappa, Sabeeha Merchant | TITLE: Enrichment of motile phototrophs by phototaxis
AUTHORS: Usha Lingappa, Sabeeha Merchant
[DESCRIPTION]
This protocol describes a method for enriching motile phototrophs from environmental samples by positive phototaxis, or the ability to move towards light. Samples are collected in the field using sterile equi... | ["[Sample collection and preparation] Collect environmental samples (e.g. soil, sediment, water, etc. ) into sterile containers (e.g. Falcon tubes or Whirl-Pak bags), using sterile equipment (gloves, spatula/scoopula).", "[Sample collection and preparation] Encourage phototrophs to bloom by incubating samples under lig... |
94,399 | Acute Extracellular Multi-unit Recordings and Optotagging | 1 | dx.doi.org/10.17504/protocols.io.14egn3qd6l5d/v1 | https://www.protocols.io/view/acute-extracellular-multi-unit-recordings-and-opto-c8e7zthn | Cristian González-Cabrera, Matthias Prigge | TITLE: Acute Extracellular Multi-unit Recordings and Optotagging
AUTHORS: Cristian González-Cabrera, Matthias Prigge
[DESCRIPTION]
This protocol details acute extracellular multi-unit recordings and optotagging in mice. Preparation involves setting up recording systems and optogenetic equipment. The mouse is anestheti... | ["[Preparation and Anesthetization of the Mouse] Set up a clean surgical area.\nEnsure all surgical tools and the multielectrode silicone probe are sterilized.", "[Preparation and Anesthetization of the Mouse] Induce anesthesia in the mouse using the isofluorane induction box.\nOnce anesthetized, place the animal in th... |
null | null | null | dx.doi.org/10.17504/protocols.io.ciyufv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
How to make a 20 mg/mL X-Gluc Stock Solution
[STEPS]
?.
?.
?. | [] |
36,844 | Isolation and Characterization of Immune Cells from the Tumor Microenvironment of Genetically Engineered Pediatric High Grade Glioma Models Using the Sleeping Beauty Transposon System | null | dx.doi.org/10.17504/protocols.io.bf8kjruw | https://www.protocols.io/view/isolation-and-characterization-of-immune-cells-fro-bf8kjruw | Maria B Garcia Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler, Pedro R Lowenstein, Maria G Castro | TITLE: Isolation and Characterization of Immune Cells from the Tumor Microenvironment of Genetically Engineered Pediatric High Grade Glioma Models Using the Sleeping Beauty Transposon System
AUTHORS: Maria B Garcia Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler, Pedro R Lowe... | [] |
89,229 | Ex vivo ATP/ADP measurements | 4 | dx.doi.org/10.17504/protocols.io.8epv5xe44g1b/v1 | https://www.protocols.io/view/ex-vivo-atp-adp-measurements-c3dmyi46 | Cecilia Tubert | TITLE: Ex vivo ATP/ADP measurements
AUTHORS: Cecilia Tubert
[DESCRIPTION]
Ex-vivo slices obtained from mice that had previously undergone viral stereotaxic injections to express genetically encoded probes are used for fluorescence microscopy experiments. Because of the thickness of the slice tissue the preferred imagi... | ["[Procedure:] - Brain slices expressing PercevalHR are obtained according to protocol and held at room temperature in a chamber containing aCSF continuously bubbled with 95% O2/5% CO2 blood gas mixture until the moment of the experiment.", "[Procedure:] - Turn on 2PLSM working station, including heated sta... |
80,076 | Slow freeze (cryopreservation) protocol for human ovarian tissue | 4 | dx.doi.org/10.17504/protocols.io.5jyl8jzr6g2w/v1 | https://www.protocols.io/view/slow-freeze-cryopreservation-protocol-for-human-ov-csfkwbkw | Elizabeth L Tsui, Monica M Laronda, Hannah McDowell | TITLE: Slow freeze (cryopreservation) protocol for human ovarian tissue
AUTHORS: Elizabeth L Tsui, Monica M Laronda, Hannah McDowell
[DESCRIPTION]
Purpose: This protocol describes the procedure for cryopreservation of organ donor derived human ovarian tissue via slow freeze with a semi-automated controlled rate freeze... | ["[Equipment set up] The controlled rate freezer has three components: computer, temperature controller - measures temperature of the tube rack, and cryobath (with internal tube rack suitable for cryovials)\n\nSet up programmable freezer by opening the relevant freezer software and programming the controlled rate freez... |
null | null | null | dx.doi.org/10.17504/protocols.io.rv9d696 | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
58,682 | Golden Gate Assembly | 4 | null | https://www.protocols.io/view/golden-gate-assembly-b5i2q4ge | Amanda T Alker, Nicholas J Shikuma | TITLE: Golden Gate Assembly
AUTHORS: Amanda T Alker, Nicholas J Shikuma
[DESCRIPTION]
Protocol for golden gate assembly of modular plasmids from Yeast Toolkit, Bee Toolkit and Marine Modification Kit platforms.
[STEPS]
SECTION: Day 1
1. Streak out the plasmid parts that will be used in the assembly onto LB agar plate... | ["[Day 1] Streak out the plasmid parts that will be used in the assembly onto LB agar plates with the appropriate antibiotics (Chloramphenicol 100µg/mL for Type 1-7 parts; Kanamycin or Gentamicin 100µg/mL for Type 8 backbone parts). Incubate overnight at 37ºC.", "[Day 2] Inoculate a single colony into 25mL of LB plus a... |
64,895 | Guardian Blood Balance Australia (ALERT SCAM) Does This Botanicals Blood Balance Formula Really Works? Read Shocking Report! | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn4qmvx9/v1 | https://www.protocols.io/view/guardian-blood-balance-australia-alert-scam-does-t-cbk7skzn | guardianbloodbalanceaust | TITLE: Guardian Blood Balance Australia (ALERT SCAM) Does This Botanicals Blood Balance Formula Really Works? Read Shocking Report!
AUTHORS: guardianbloodbalanceaust
[DESCRIPTION]
Guardian Blood Balance is available in capsule form. The capsules are made in the United States in a GMP certified facility and FDA-appro... | ["Guardian Blood Balance (Australia) is a blood-sugar management supplement that works. It is useful for helping patients to keep their cholesterol and blood sugar levels in balance. This product can be used to treat diabetes, high blood sugar, cholesterol and other related diseases. Anyone who has these conditions can... |
28,913 | Plate reader setting | null | dx.doi.org/10.17504/protocols.io.8grhtv6 | null | Junyan Qian | TITLE: Plate reader setting
AUTHORS: Junyan Qian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A brief guideline of setting the plate reader to measure the sfGFP production.</div></div>
[STEPS]
?. Create a new protocol in PerkinElmer by clicking start wizard in Victor Workstation. Name it as the ... | ["Create a new protocol in PerkinElmer by clicking start wizard in Victor Workstation. Name it as the substance you want to measure. In our case sfGFP, select a folder to save the protocol. Click Next>.", "Select the plate you are using for the measurement, it allows the plate reader to have a default setting of the po... |
35,968 | SARS-CoV-2 detection using BGI RT-PCR kit | null | dx.doi.org/10.17504/protocols.io.bfc8jizw | https://www.protocols.io/view/sars-cov-2-detection-using-bgi-rt-pcr-kit-bfc8jizw | Wei-Ting Lu, Ming Jiang, Robert Goldstone, Karen Ambrose, Chris Ekin, Amy Strange, Nnenna Kanu, Paul Grant, Efthymios Fidanis, Jerome Nicod, David Moore, Michael Howell | TITLE: SARS-CoV-2 detection using BGI RT-PCR kit
AUTHORS: Wei-Ting Lu, Ming Jiang, Robert Goldstone, Karen Ambrose, Chris Ekin, Amy Strange, Nnenna Kanu, Paul Grant, Efthymios Fidanis, Jerome Nicod, David Moore, Michael Howell
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weigh... | ["[RNA Transfer: Biomek FX Setup]\nOpen the Biomek software, and select the program “qPCR Setup” which is located in the “Covid-19” project:\nNote: The RNA transfer into PCR plate can also be done manually with a multichannel pipette with filtered tips in a prepared PCR Hood (10 μl of RNA from RNA plate into correspon... |
56,933 | Ultrasound 96 Probe Device Protocol for cancer cell treatment | 1 | dx.doi.org/10.17504/protocols.io.b3udqns6 | https://www.protocols.io/view/ultrasound-96-probe-device-protocol-for-cancer-cel-b3udqns6 | Aisling Field, Brijesh Tiwari, James F Curtin, Julie Rose Mae Mondala, Janith Wanigasekara | TITLE: Ultrasound 96 Probe Device Protocol for cancer cell treatment
AUTHORS: Aisling Field, Brijesh Tiwari, James F Curtin, Julie Rose Mae Mondala, Janith Wanigasekara
[DESCRIPTION]
Ultrasound is a sound wave with frequencies ranging between 20 kHz and 20 MHz. Ultrasound is able to temporarily and repeatedly op... | ["Before starting the ultrasound probe system, ensure that all parts of the system/device are free from mechanical damage and that the probe is connected tightly.", "The 96-probe system is designed to fit perfectly into the 96 well plate, which can be used to grow and treat cancer cells. The retort stand is used to hol... |
43,643 | Mini culture slants for long term storage of fungi | 3 | dx.doi.org/10.17504/protocols.io.bnu3meyn | https://www.protocols.io/view/mini-culture-slants-for-long-term-storage-of-fungi-bnu3meyn | You Li, Jiri Hulcr | TITLE: Mini culture slants for long term storage of fungi
AUTHORS: You Li, Jiri Hulcr
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to store fungi in slants.</div><div class = "text-block"><span>This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordinat... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.chpt5m | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
97,610 | SOP – 3-step protein fractionation from fly heads | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8emolmk/v1 | https://www.protocols.io/view/sop-3-step-protein-fractionation-from-fly-heads-dbji2kke | ALFONSO.M.PENA | TITLE: SOP – 3-step protein fractionation from fly heads
AUTHORS: ALFONSO.M.PENA
[DESCRIPTION]
SOP – 3-step protein fractionation from fly heads
[STEPS]
SECTION: Protocol:
7. Collect 20-40 fly heads via Snap Freeze method into a Biomasher tube and transfer tubes to CTRND on dry ice.
SECTION: Protocol:
8. Turn on Sorv... | ["[Protocol:] Collect 20-40 fly heads via Snap Freeze method into a Biomasher tube and transfer tubes to CTRND on dry ice.", "[Protocol:] Turn on Sorvall ultracentrifuge and set temperature to 4degC, place rotor inside and turn on vacuum to allow internal temperature to set.", "[Protocol:] Add 50uL (2.5uL/fly head) of ... |
68,913 | Sample submission for LC-MS BioMS core facility at the University of Manchester | 1 | dx.doi.org/10.17504/protocols.io.dm6gpb4qdlzp/v1 | https://www.protocols.io/view/sample-submission-for-lc-ms-bioms-core-facility-at-cfirtkd6 | ronan.ocualain | TITLE: Sample submission for LC-MS BioMS core facility at the University of Manchester
AUTHORS: ronan.ocualain
[DESCRIPTION]
Submitting samples to the BioMS core facility.
[BEFORE_START]
Peptides should be dried down, use the speed vac in lab B2075 for this purpose.
Instructions are here
[GUIDELINES]
Peptides should... | ["Place the dried down peptides into the drawer in B2071, either in a labelled bag (<20), or box if you are submitting a large batch of samples.\n\nOn the label, you must include \nPPMS order number (not project), \nnumber of samples, \nand any other important details, such as run length, \nsample manifest (i.e. which ... |
90,558 | Protocol to isolate and fix nuclei from flash frozen mouse hypothalamus and pituitary gland for IGVF | 4 | null | https://www.protocols.io/view/protocol-to-isolate-and-fix-nuclei-from-flash-froz-c4n6yvhe | Elisabeth Rebboah | TITLE: Protocol to isolate and fix nuclei from flash frozen mouse hypothalamus and pituitary gland for IGVF
AUTHORS: Elisabeth Rebboah
[DESCRIPTION]
This protocol describes isolation of nuclei from 10 week old mouse hypothalamus and pituitary gland (tissue ID: 01) from 8 founder strains (B6J, AJ, 129S1J, NZOJ, WSBJ, N... | ["[Setup] Coat SHARE-seq nuclei prep tubes with BSA. Fill 8 1.5 ml tubes with 1.5 ml 1% BSA-DEPC and incubate for 30 minutes. After incubation, aspirate BSA solution and dry for 30 minutes. Store at 4C.", "[Setup] Label tubes.", "[Setup] Pre-chill centrifuge to 4C.", "[Setup] Prepare ice buckets.", "[Setup] Prepare 35 ... |
92,898 | DNA extraction NucleoSpin Tissue INRAE eWHALE | 1 | dx.doi.org/10.17504/protocols.io.rm7vzxexrgx1/v1 | https://www.protocols.io/view/dna-extraction-nucleospin-tissue-inrae-ewhale-c6yazfse | Teddy Urvois, Anne-Laure Besnard, Erwan Quéméré | TITLE: DNA extraction NucleoSpin Tissue INRAE eWHALE
AUTHORS: Teddy Urvois, Anne-Laure Besnard, Erwan Quéméré
[DESCRIPTION]
This DNA extraction protocol aims at extracting total genomic DNA from filtered seawater samples suspended in Tris-EDTA-SDS Buffer.
Our protocol mostly follows the NucleoSpin Tissue protocol (q... | ["[Lyse sample] Add 200 µL of to a microcentrifuge tube.\nAdd 200 µL Buffer B3 and 25 µL Proteinase K solution.\nVortex vigorously.\nIncubate at 70 °C for 10 min.\nVortex briefly.", "[Adjust DNA binding conditions] Add 210 µL ethanol (96 – 100 %) to the sample and vortex vigorously.", "[Bind DNA] For each , place on... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.