id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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76,248 | Immunofluorescence-based assay to assess LRRK2 recruitment by Rab29 | 4 | dx.doi.org/10.17504/protocols.io.bp2l69r8klqe/v1 | https://www.protocols.io/view/immunofluorescence-based-assay-to-assess-lrrk2-rec-cnpyvdpw | Francesca Tonelli | TITLE: Immunofluorescence-based assay to assess LRRK2 recruitment by Rab29
AUTHORS: Francesca Tonelli
[DESCRIPTION]
Previous studies using confocal fluorescence microscopy showed that transient over-expression of Rab29 recruits the bulk of cellular LRRK2 to the Golgi, which results in its activation (PMID: 29212815). ... | ["[Plating cells] In a laminar flow hood for cell culture, remove culture medium from one flask of HEK293 cells.", "[Preparing coverslips] Sterilise the glass coverslips by immersing in 100% ethanol for at least 30 minutes.", "[Preparing coverslips] Leave the coverslips to air dry completely in the laminar flow hood.",... |
null | null | null | dx.doi.org/10.17504/protocols.io.c5ey3d | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Can be used for diluting or resuspending phage preparations.
[GUIDELINES]
Materials needed:<br />NaCl (MW=58.44)<br />Tris-Cl (MW=157.64)<br />MgSO<sub>4</sub>-7H<sub>2</sub>O (MW=246.47)<br />Milli Q water<br /><br />This buffer can be prepared from powdered chemicals or from ... | [] |
67,742 | PROTOCOL – Availability of Open Access Metadata from Open Journals – A case study in DOAJ and Crossref | 1 | dx.doi.org/10.17504/protocols.io.kxygxz7ywv8j/v5 | https://www.protocols.io/view/protocol-availability-of-open-access-metadata-from-ced6ta9e | Davide Brembilla, Chiara Catizone, Giulia Venditti | TITLE: PROTOCOL – Availability of Open Access Metadata from Open Journals – A case study in DOAJ and Crossref
AUTHORS: Davide Brembilla, Chiara Catizone, Giulia Venditti
[DESCRIPTION]
This protocol is for the research about the availability of Metadata from Open Journals in the DOAJ in Crossref.
The goal is to find ou... | ["[‱ Data Gathering] Download DOAJ articles metadata\n\nFor starting our research, we first focused on retrieving and cleaning DOAJ articles' data as we identified it as the starting point from which we could look for an answer to our research questions.", "[‱ Data Gathering] We downloaded the DOAJ public data dump con... |
105,014 | Beam traversal | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1212lmk/v1 | https://www.protocols.io/view/beam-traversal-disw4efe | Livia Hecke Morais | TITLE: Beam traversal
AUTHORS: Livia Hecke Morais
[DESCRIPTION]
This test measures the time for mice to cross a 1-meter plexiglass beam with segments decreasing
in width (3.5 cm, 2.5 cm, 1.5 cm, 0.5 cm). Mice were trained for two days
before testing. On testing day, mice were placed at the start of the 3.5 cm
segment ... | ["[Information] Experiments involving GF and GF x SPF comparisons were conducted inside germ-free isolators. For comparisons involving only SPF animals, experiments were completed in a biological safety cabinet. All experiments were performed between ZT6 and ZT10 of the light phase and started at the same time each day... |
36,631 | PONE-D-19-35784 Proximal renal tubular function in HIV-infected children on tenofovir disoproxil fumarate for treatment of HIV infection at two tertiary hospitals in Harare, Zimbabwe | 1 | dx.doi.org/10.17504/protocols.io.bfzxjp7n | https://www.protocols.io/view/pone-d-19-35784-proximal-renal-tubular-function-in-bfzxjp7n | Runyararo Mano | TITLE: PONE-D-19-35784 Proximal renal tubular function in HIV-infected children on tenofovir disoproxil fumarate for treatment of HIV infection at two tertiary hospitals in Harare, Zimbabwe
AUTHORS: Runyararo Mano
[STEPS]
?. AbstractThis laboratory protocol describes urine and blood sample collection and measuring th... | ["AbstractThis laboratory protocol describes urine and blood sample collection and measuring the concentration of urine glucose and protein, urine protein creatinine ratio, urine, and serum phosphate as well as urine and serum creatinine to determine the prevalence of proteinuria, estimated glomerular filtration rat... |
45,816 | Brad-seq mRNA (for Shotgun or DGE) | 4 | dx.doi.org/10.17504/protocols.io.bqyymxxw | https://www.protocols.io/view/brad-seq-mrna-for-shotgun-or-dge-bqyymxxw | Yin-Tse Huang | TITLE: Brad-seq mRNA (for Shotgun or DGE)
AUTHORS: Yin-Tse Huang
[STEPS]
?. [Tissue Lysis]
Wipe off RNAlatter from tissue; Place of tissue in crushing tube with metal cone
20 mg
?. [Tissue Lysis]
Add of 2-Mercaptoethanol (2-ME) to of LBB (for ratio, adjust it for the amount LBB used)
5 µl
1 mL
?. [Tissue Lysis]
Add ... | ["[Tissue Lysis]\nWipe off RNAlatter from tissue; Place of tissue in crushing tube with metal cone\n20 mg", "[Tissue Lysis]\nAdd of 2-Mercaptoethanol (2-ME) to of LBB (for ratio, adjust it for the amount LBB used)\n5 µl\n1 mL", "[Tissue Lysis]\nAdd in tube\n[100/10: LBB/Tissue ratio]", "[Tissue Lysis]\nCrush sample ... |
null | null | null | dx.doi.org/10.17504/protocols.io.rwtd7en | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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95,121 | Sanger Tree of Life HMW DNA Extraction: Automated MagAttract for Small Arthropods | 1 | dx.doi.org/10.17504/protocols.io.ewov1qp42gr2/v1 | https://www.protocols.io/view/sanger-tree-of-life-hmw-dna-extraction-automated-m-c85rzy56 | Amy Denton, Abitha Thomas, Caroline Howard | TITLE: Sanger Tree of Life HMW DNA Extraction: Automated MagAttract for Small Arthropods
AUTHORS: Amy Denton, Abitha Thomas, Caroline Howard
[DESCRIPTION]
This protocol describes the automated extraction and SPRI of HMW DNA from small arthropod samples intended for long-read sequencing using the Qiagen MagAttract HMW ... | ["[Sample Lysis] Prepare a lysis buffer mastermix:", "[Sample Lysis] For samples which have been cryogenically homogenised via cryoPREP:\nTransfer 25 mg of the sample into a 2 mL microcentrifuge tube, then hold on dry ice to keep the sample frozen.\nAdd 187 µL of the lysis buffer master mix to sample, then homogenise t... |
107,136 | Deep-learning tool for early identification of non-traumatic intracranial hemorrhage etiology based on CT scan | 0 | dx.doi.org/10.17504/protocols.io.yxmvmejoog3p/v1 | https://www.protocols.io/view/deep-learning-tool-for-early-identification-of-non-dku84wzw | Meng Zhao | TITLE: Deep-learning tool for early identification of non-traumatic intracranial hemorrhage etiology based on CT scan
AUTHORS: Meng Zhao
[DESCRIPTION]
Objective
To develop an
artificial intelligence system that can accurately identify acute non-traumatic
intracranial hemorrhage (ICH) etiology based on non-contrast CT ... | ["Dataset and Clinical Taxonomy Retrospectively review NCCT scans from 4019 patients at Beijing Tiantan Hospital (2011-2018). Include only the first NCCT scan post-ICH event; exclude traumatic, post-surgical, or delayed scans.", "Inclusion and Exclusion Criteria Exclude scans with poor quality, lacking a complete ser... |
null | null | null | dx.doi.org/10.17504/protocols.io.gstbwen | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p>This protocol describes a cell viability assay that uses near-infrared fluorescent detection. Sapphire700 Stain is used to determine cell viability by assessing cell membrane integrity, and the as... | ["[Cell Preparation] Grow A431 cells in a 100-mm tissue culture dish with growth medium (DMEM supplemented with 10% FBS) using standard cell culture practices. Always make sure that cells are healthy before using them for the experiment.", "[Saponin Treatment] {\"blocks\":[{\"key\":\"f8s9a\",\"text\":\" The day befor... |
89,372 | Plasmid construction | 1 | dx.doi.org/10.17504/protocols.io.bp2l6x3b5lqe/v1 | https://www.protocols.io/view/plasmid-construction-c3h4yj8w | Minghao Chen, Xuefeng Ren | TITLE: Plasmid construction
AUTHORS: Minghao Chen, Xuefeng Ren
[DESCRIPTION]
plasmid construction
[STEPS]
1. Amplify the insert gene fragment by PCR with primers including 21 nt of overlapping sequence with the target gene.
2. Linearize the backbone with restriction enzymes (NEB).
3. Treat linearized backbone with... | ["Amplify the insert gene fragment by PCR with primers including 21 nt of overlapping sequence with the target gene.", "Linearize the backbone with restriction enzymes (NEB).", "Treat linearized backbone with quick CIP (NEB).", "Run PCR products and linearized backbone in an agarose gel to confirm the size.", "Purify ... |
null | null | null | dx.doi.org/10.17504/protocols.io.gvgbw3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p style="text-align: center;"><img id="s-mce-img" class="s-mce-img" style="display: block; margin-left: auto; margin-right: auto;" src="https://s3.amazonaws.com/pr-journal/hnnfjtn.png" width="100" height="90" data-src="https://s3.amazonaws.com/pr-journal/hnnfjtn.png" data-ofn="... | [] |
21,292 | Yale - Blood Glucose | null | dx.doi.org/10.17504/protocols.io.y2kfycw | null | John Stack, Gary Cline | TITLE: Yale - Blood Glucose
AUTHORS: John Stack, Gary Cline
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Procedure used to measure glucose concentrations. Glucose is measured by the enzymatically coupled reactions o... | ["Calibrate Cobas for Glucose analysis by running a multi analyte standard and two control serum.", "Sample Handling as performed by the Cobas Mira Plus. a) Pipette 3 µL of sample into cuvette. b) Absorbance is measured at 340 nm. c) Add 100 µL of Glucose liquid reagent. d) Mixture is incubated at 37ºC for ... |
null | null | null | dx.doi.org/10.17504/protocols.io.qrgdv3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>RNA from matched FFPE, PFPE and cryo preserved human tissues, stored for up to seven years at 22°C and 4°C, examined for integrity and usability in quantitative RT-PCR</p>
[STEPS]
?. | [] |
52,713 | A Mixed-Methods Catchment Area Modelling Approach Using Free DEMs to Inform Environmental Surveillance in Kolkata, India and Accra, Ghana | 1 | dx.doi.org/10.17504/protocols.io.dm6gpwkxdlzp/v1 | https://www.protocols.io/view/a-mixed-methods-catchment-area-modelling-approach-bxqhpmt6 | Jamie VanTassell, Christine Moe | TITLE: A Mixed-Methods Catchment Area Modelling Approach Using Free DEMs to Inform Environmental Surveillance in Kolkata, India and Accra, Ghana
AUTHORS: Jamie VanTassell, Christine Moe
[DESCRIPTION]
Environmental surveillance (ES) is an effective epidemiological tool that has successfully been used to monitor polio... | ["[Case Study #1: Kolkata, India] Extracting Waterways from OpenStreetMap\nFreely available DEMs have a resolution quality of 30 meters (30-m by 30-m pixels). This resolution is oftentimes too low for ArcGIS to detect all of the waterways, particularly in densely populated areas with flat terrain. To improve the qualit... |
null | null | null | dx.doi.org/10.17504/protocols.io.cr5v85 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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92,191 | Automated 96 well plate based protein quantitation using a Beckman Biomek™ NxP workstation and a Pierce™ 660nm Protein Assay Kit | 1 | dx.doi.org/10.17504/protocols.io.4r3l2215ql1y/v1 | https://www.protocols.io/view/automated-96-well-plate-based-protein-quantitation-c597y99n | ronan.ocualain | TITLE: Automated 96 well plate based protein quantitation using a Beckman Biomek™ NxP workstation and a Pierce™ 660nm Protein Assay Kit
AUTHORS: ronan.ocualain
[DESCRIPTION]
These include
sample lysis,
protein extraction,
solubilisation,
estimation,
reduction and alkylation,
normalisation,
clean-up,
enzymatic ... | ["[Deck layout] Add the sample plate to be measured in P1 of the deck.", "[Deck layout] To a Beckman full reservoir, add 50 mL of Pierce 660nm protein assay reagent with 1 g of ionic detergent compatibility reagent (IDCR). This can be prepared by adding the 1g from a packet of IDCR to a 50 mL falcon tube, and adding 50... |
86,093 | Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry | 1 | null | https://www.protocols.io/view/preparation-of-soluble-and-insoluble-mitochondrial-cybmxsk6 | Louise Uoselis | TITLE: Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry
AUTHORS: Louise Uoselis
[DESCRIPTION]
Preparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for mass spectrometry
[STEPS]
SECTION: Day 1
1. Thaw mitochondrial stocks on ice, and aliquot out... | ["[Day 1] Thaw mitochondrial stocks on ice, and aliquot out one tube of 60 µg of mitochondria for each sample.", "[Day 1] Centrifuge samples at 10,000x rcf for 10 min at 4 deg C, and carefully aspirate the supernatant", "[Day 1] Add 60 µL of ice cold 0.5% TX-100 in PBS, vortex each sample for ~5 seconds, and leave samp... |
101,358 | rtWIZARD Protocol with the Opentrons OT-2 v1 | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8813lmk/v1 | https://www.protocols.io/view/rtwizard-protocol-with-the-opentrons-ot-2-v1-de8n3hve | Rohan Laurent, Benjamin Hinnant, Michael D. Talbott, Kenneth Kim | TITLE: rtWIZARD Protocol with the Opentrons OT-2 v1
AUTHORS: Rohan Laurent, Benjamin Hinnant, Michael D. Talbott, Kenneth Kim
[DESCRIPTION]
Starting with sample tubes racked in a 48-well plate, this rtWIZARD protocol uses the Opentrons OT-2 to automate performance of up to 42 rapid tests(RTs) or lateral flow assays. F... | ["[Testing grid and board preparation] Print the four pages in the deck printouts file (Appendix 1, Fig 1a).", "[Testing grid and board preparation] Using ruler, measure the 140mm scale bar on grid printouts to verify that dimensions are true. If dimensions need adjusting, adjust scale in your printer settings until di... |
59,714 | Useful methods: Herbarium of duckweed specimen | 1 | null | https://www.protocols.io/view/useful-methods-herbarium-of-duckweed-specimen-b6jarcie | K. Sowjanya Sree, Klaus-J. Appenroth | TITLE: Useful methods: Herbarium of duckweed specimen
AUTHORS: K. Sowjanya Sree, Klaus-J. Appenroth
[DESCRIPTION]
This protocol details about preparation of herbaria for duckweed specimens. It contains protocols from the The International Steering Committee on Duckweed Research and Application (ISCDRA) Newsletter. A c... | ["[Method] For preparing herbarium samples, select the representative clones of each of the duckweed\nspecies from the stock cultures at the University of Jena, Germany (Sree and Appenroth,\n2020).", "[Method] Cultivate under optimal conditions for four weeks by sub-culturing them each week. Use the plants from such we... |
45,240 | SPOT2 revised protocol | 4 | dx.doi.org/10.17504/protocols.io.bqeymtfw | https://www.protocols.io/view/spot2-revised-protocol-bqeymtfw | Huimin Zhao, stlane2 , Guanhua Xun | TITLE: SPOT2 revised protocol
AUTHORS: Huimin Zhao, stlane2 , Guanhua Xun
[STEPS]
?. [SPOT testing protocol]
Prepare a new test kit and collect a patient saliva sample.
?. [SPOT testing protocol]
Perform sample pretreatment for 5 minutes at 95°C.If using water baths: Place P-tube in floating rack and insert rack into ... | ["[SPOT testing protocol]\nPrepare a new test kit and collect a patient saliva sample.", "[SPOT testing protocol]\nPerform sample pretreatment for 5 minutes at 95°C.If using water baths: Place P-tube in floating rack and insert rack into sous vide water bath preheated to 95°C. Incubate for 5 minutes for sample pretrea... |
null | null | null | dx.doi.org/10.17504/protocols.io.dqj5um | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For <a href="https://www.protocols.io/view/Enumeration-of-virus-particles-in-aquatic-or-sedim-dpy5pv" target="_blank">Enumeration of virus particles in aquatic or sediment samples by epifluorescence microscopy</a> protocol.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
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?. | [] |
54,682 | Intravenous (tail vein) injection of AAV into rat | 1 | dx.doi.org/10.17504/protocols.io.6qpvrdbbpgmk/v1 | https://www.protocols.io/view/intravenous-tail-vein-injection-of-aav-into-rat-bzm2p48e | Janet R Keast, Nicole Wiedmann, Peregrine B Osborne | TITLE: Intravenous (tail vein) injection of AAV into rat
AUTHORS: Janet R Keast, Nicole Wiedmann, Peregrine B Osborne
[DESCRIPTION]
This protocol is used to administer adeno-associated virus (AAV) in male and female rats. This procedure is performed under anesthesia and should incorporate all local requirements for st... | ["[Preparation] Determine the dose of virus to administer per rat. Divide the dose (viral genomes (vg)) by the titre (vg/ml) to calculate the volume of virus needed to inject one rat. \n\nViral dosage can be determined using:", "[Preparation] In a screw-cap vial, prepare the solution to be administered. Add virus, and... |
null | null | null | dx.doi.org/10.17504/protocols.io.qtndwme | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div style="left: 45.1137px; top: 176.402px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.955321);">Transformation & Storage Solution (2X TSS) enables researchers to take advantage of the simple system described by Chung et al. <sup>1</sup> for the preparati... | [] |
101,924 | iPSC Midbrain differentiation in 96 well plates | 1 | dx.doi.org/10.17504/protocols.io.bp2l62dj5gqe/v1 | https://www.protocols.io/view/ipsc-midbrain-differentiation-in-96-well-plates-dfsc3naw | gustavo.parfitt Parfitt, Elena Coccia | TITLE: iPSC Midbrain differentiation in 96 well plates
AUTHORS: gustavo.parfitt Parfitt, Elena Coccia
[DESCRIPTION]
Midbrain differentiation protocol using spinner flasks.
[STEPS]
SECTION: iPSCs plating for differentiation
1. Passage 1X106 iPSC into Geltrex coated 10-cm dish with Accutase and rock inhibitor (refer to... | ["[iPSCs plating for differentiation] Passage 1X106 iPSC into Geltrex coated 10-cm dish with Accutase and rock inhibitor (refer to material section).", "[iPSCs plating for differentiation] For the passaging add 3 mL of Accutase for 5 min .", "[iPSCs plating for differentiation] Add 5 mL of StemFlex , gently mix and tr... |
87,502 | JMN-MSMP Muscle bulk RNA seq | 1 | null | https://www.protocols.io/view/jmn-msmp-muscle-bulk-rna-seq-czpnx5me | ccherry | TITLE: JMN-MSMP Muscle bulk RNA seq
AUTHORS: ccherry
[DESCRIPTION]
RNA sequencing of muscle
[STEPS]
SECTION: Bulk RNA sequencing
1. cDNA synthesis and library preparation were performed using the TruSeq RNA Library Prep Kit V2 following the manufacturer's protocol.
SECTION: Bulk RNA sequencing
2. The Agilent BioAnal... | ["[Bulk RNA sequencing] cDNA synthesis and library preparation were performed using the TruSeq RNA Library Prep Kit V2 following the manufacturer's protocol.", "[Bulk RNA sequencing] The Agilent BioAnalyzer system was used to perform quality control on samples during the library prep process as suggested in the manufac... |
86,802 | Bulk RNASeq Delivery | 5 | null | https://www.protocols.io/view/bulk-rnaseq-delivery-cyzsxx6e | Tyler Stahl | TITLE: Bulk RNASeq Delivery
AUTHORS: Tyler Stahl
[DESCRIPTION]
This protocol will give an overview of the grc data delivery structure and results files
[STEPS]
SECTION: Analysis Overview
1. In brief, the RNASeq analysis follows pre-processing (quality control/filtering/trimming), alignment, post-processing, and dif... | ["[Analysis Overview] In brief, the RNASeq analysis follows pre-processing (quality control/filtering/trimming), alignment, post-processing, and differential expression analysis between sample groups with DESeq2. A detailed overview of the analysis can be found in the README.txt and methods.txt file. Also, please see ... |
61,607 | Quantitative real-time PCR of mRNA | 4 | dx.doi.org/10.17504/protocols.io.q26g74xoqgwz/v1 | https://www.protocols.io/view/quantitative-real-time-pcr-of-mrna-b8efrtbn | Laura Smith | TITLE: Quantitative real-time PCR of mRNA
AUTHORS: Laura Smith
[DESCRIPTION]
Total RNA was extracted from dry cell pellets using the RNAeasy kit (Qiagen), per manufacturer’s instructions. Concentration and purity of ribonucleic acid (RNA) was determined using the Nanodrop. 600 ng RNA was converted to cDNA with the Qu... | ["Protocol adapted from: Matthew E Gegg, Guglielmo Verona, Anthony H V Schapira, Glucocerebrosidase deficiency promotes release of α-synuclein fibrils from cultured neurons,Human Molecular Genetics, Volume 29, Issue 10, 15 May 2020, Pages 1716–1728,https://doi.org/10.1093/hmg/ddaa085", "[RNA purification] Total RNA was... |
9,582 | MMH1 Solution | 1 | dx.doi.org/10.17504/protocols.io.mknc4ve | https://www.protocols.io/view/mmh1-solution-mknc4ve | Erçağ Pinçe | TITLE: MMH1 Solution
AUTHORS: Erçağ Pinçe
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Minimal medium H1 protocol for bacterial competition experiments</div></div>
[STEPS]
?. [Prepare stock solutions of the salts]
Prepare Potassium Phosphate: ABCDEFGHI1Name of the chemicalFormulaBrandMolecular... | ["[Prepare stock solutions of the salts]\nPrepare Potassium Phosphate: ABCDEFGHI1Name of the chemicalFormulaBrandMolecular Weight(gr/mol)Final ConcentrationStockFactor Stock in 100mL in gr 2Potassium phosphate(Monobasic) KH2PO4Sigma-Aldrich139.06 50 mM 1M 20x9.5 3Potassium Pho... |
35,783 | An archaeological experiment focused on the intuitive way of long bones breakage to extract marrow | null | dx.doi.org/10.17504/protocols.io.be7fjhjn | https://www.protocols.io/view/an-archaeological-experiment-focused-on-the-intuit-be7fjhjn | Delphine Vettese | TITLE: An archaeological experiment focused on the intuitive way of long bones breakage to extract marrow
AUTHORS: Delphine Vettese
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-style:italic;">English:</span></div><div class = "text-block">This protocol was carried out followin... | ["[Workstation Preparation]\nEnglish:Material installation: extend the plastic sheet and provide the tools (hammer, anvil, plate, wooden sticks)French:Installation du matériel: étendre la bâche et mettre à disposition les outils (percuteur, enclume, assiette, bâtons de bois)", "[Workstation Preparation]\nEnglish:Place ... |
66,311 | [UPDATED] Prima Pills Review {Warnings}: Scam, 10 Facts Side Effects, Does It Work? | 3 | dx.doi.org/10.17504/protocols.io.n92ldzxyov5b/v1 | https://www.protocols.io/view/updated-prima-pills-review-warnings-scam-10-facts-cczfsx3n | Prima Weight Loss Pills | TITLE: [UPDATED] Prima Pills Review {Warnings}: Scam, 10 Facts Side Effects, Does It Work?
AUTHORS: Prima Weight Loss Pills
[DESCRIPTION]
Prima Weight Loss Pills
[STEPS] | [] |
86,976 | Protocol to isolate and fix nuclei from flash frozen mouse tibialis anterior for IGVF | 4 | null | https://www.protocols.io/view/protocol-to-isolate-and-fix-nuclei-from-flash-froz-cy68xzhw | Elisabeth Rebboah | TITLE: Protocol to isolate and fix nuclei from flash frozen mouse tibialis anterior for IGVF
AUTHORS: Elisabeth Rebboah
[DESCRIPTION]
This protocol describes isolation of nuclei from 10 week old mouse tibialis anterior from 8 founder strains (B6J, AJ, 129S1J, NZOJ, WSBJ, NODJ, PWKJ, and CASTJ), preparation of a single... | ["[Setup] Pre-chill centrifuge to 4C.", "[Setup] Prepare 2 large ice buckets.", "[Setup] Prepare lysis buffer on ice in a 50 mL conical tube. Distribute 2.5 mL into 8 gentleMACS C Tubes on ice.", "[Setup] Prepare 4 mL NB + BSA + RNAse inhibitor in a 5 mL tube.", "[Setup] Prepare 25 mL RSB on ice in a 50 mL conical tube... |
25,701 | RPA from banana DNA | null | dx.doi.org/10.17504/protocols.io.5cdg2s6 | null | Théo Nass, Luc Gabel | TITLE: RPA from banana DNA
AUTHORS: Théo Nass, Luc Gabel
[STEPS]
?. [Banana DNA extraction]
Put chopped banana in a closed ziploc-like bag
?. [Banana DNA extraction]
Add diluted 1:10 dish soap solution and 1g table salt to fruits
?. [Banana DNA extraction]
Mash it until homogeneous mixture is obtained
?. [Banana DNA e... | ["[Banana DNA extraction]\nPut chopped banana in a closed ziploc-like bag", "[Banana DNA extraction]\nAdd diluted 1:10 dish soap solution and 1g table salt to fruits", "[Banana DNA extraction]\nMash it until homogeneous mixture is obtained", "[Banana DNA extraction]\nStrain the mixture through a household coffee filter... |
73,826 | Protocol for Cross-species characterization in the reproduction of Spodoptera sunia (Lepidoptera: Noctuidae) | 4 | dx.doi.org/10.17504/protocols.io.81wgbyzk3vpk/v2 | https://www.protocols.io/view/protocol-for-cross-species-characterization-in-the-ckcausse | Carlos Ivan Real Baca, Carlos Alberto Zuniga Gonzalez | TITLE: Protocol for Cross-species characterization in the reproduction of Spodoptera sunia (Lepidoptera: Noctuidae)
AUTHORS: Carlos Ivan Real Baca, Carlos Alberto Zuniga Gonzalez
[DESCRIPTION]
The Protocol describes the process for Cross-species Characterization in the reproduction of Spodoptera Sunia(Lepidoptera: Noc... | ["Stage 1: Field tracking\nThe search for Spodoptera sunia was carried out in an onion plantation in Sébaco (12.87845, -86.09147), it was inspected in the upper and lower part of the leaves, larvae of different instars were collected, later they were placed in polyethylene cups of 4 and 16 ounces with the leaves and bu... |
33,917 | Symbiodiniaceae Enumeration in Ground Coral Samples Using Countess™ II FL Automated Cell Counter | null | dx.doi.org/10.17504/protocols.io.bdc5i2y6 | null | Rowan Mclachlan, Claire Juracka, Andrea Grottoli | TITLE: Symbiodiniaceae Enumeration in Ground Coral Samples Using Countess™ II FL Automated Cell Counter
AUTHORS: Rowan Mclachlan, Claire Juracka, Andrea Grottoli
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol outlines a method for quantifying the density of Symbiodiniaceae cells in Sc... | ["[Separate Symbiodiniaceae from coral skeleton]\nPrepare salt water. Using a glass graduated cylinder, dispense 250 ml of Milli-Q water into a glass beaker. Using weighing paper and a balance, weigh 9 g of Instant Ocean sea salt (or sodium chloride powder) and add to the glass beaker. Using a clean metal spatula, stir... |
null | null | null | dx.doi.org/10.17504/protocols.io.fd7bi9n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A simple protocol for staining with Methylene Blue, a very commonly available stain. </p>
[STEPS]
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?.
?. | [] |
81,463 | 10x Protocols: Visium v2 CytAssist FFPE Library Construction -- University of Minnesota TMCs (CG000495 Rev B) | 1 | dx.doi.org/10.17504/protocols.io.n2bvj872ngk5/v1 | https://www.protocols.io/view/10x-protocols-visium-v2-cytassist-ffpe-library-con-ctsxwnfn | IOx Genomics, Laura J Niedernhofer, David A Bernlohr | TITLE: 10x Protocols: Visium v2 CytAssist FFPE Library Construction -- University of Minnesota TMCs (CG000495 Rev B)
AUTHORS: IOx Genomics, Laura J Niedernhofer, David A Bernlohr
[DESCRIPTION]
Protocols from 10x Genomics for Visium Spatial Gene Expression v2 chemistry on FPPE samples with the CytAssist component.
Pro... | ["10x protocol CG000 495, Revision B (Library construction with CytAssist)", "Additional Protocols/Resources\nhttps://www.10xgenomics.com/support/spatial-gene-expression-ffpe"] |
72,731 | Loading and Execution of Automatic Watering and Weighing | 1 | dx.doi.org/10.17504/protocols.io.q26g7ypp3gwz/v1 | https://www.protocols.io/view/loading-and-execution-of-automatic-watering-and-w-ci93uh8n | Magdalena M Julkowska, Olga Khmelnitsky, Hayley Sussman | TITLE: Loading and Execution of Automatic Watering and Weighing
AUTHORS: Magdalena M Julkowska, Olga Khmelnitsky, Hayley Sussman
[DESCRIPTION]
This is part of a bigger phenotyping project at the Julkowska lab in Boyce Thompson Institute in Ithaca,NY.
[STEPS]
SECTION: Loading the Experiment
1. Download the AAWsmobo... | ["[Loading the Experiment] Download the AAWsmobox.ino code from GitHub (insert link here) to a location in your computer that is easy to access with all other Arduino codes that you will be using.", "[Loading the Experiment] Open Arduino IDE and open AAWsmobox.ino", "[Loading the Experiment] After completing the calibr... |
97,023 | Plug Removal for acute in vivo Electrophysiology Experiments | 0 | dx.doi.org/10.17504/protocols.io.eq2lywz8qvx9/v1 | https://www.protocols.io/view/plug-removal-for-acute-in-vivo-electrophysiology-e-day72fzn | Ryan Gillis, Mikayla Carlson, Severine Durand | TITLE: Plug Removal for acute in vivo Electrophysiology Experiments
AUTHORS: Ryan Gillis, Mikayla Carlson, Severine Durand
[DESCRIPTION]
This protocol describes the procedure for removing the SORTA-clear plug used in acute in vivo electrophysiology experiments in whole-hemisphere craniotomy (WHC) preparation mice with... | ["[Prepare the surgical rig and anesthesia system] Ensure the vacuum gauge is set to 10-10 pounds per square inch (PSI) and that all vacuum lines are functioning correctly for both the induction chamber and nosecone scoop.", "[Prepare the surgical rig and anesthesia system] Ensure the oxygen regulator is set to 0.8-1 L... |
null | null | null | dx.doi.org/10.17504/protocols.io.rkzd4x6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
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<p>This protocol was designed and developed at this laboratory by Greg A. Smith and colleagues for Public Helath Virology, Queensland's Department of Health, prior to his departure. It has not been pre... | [] |
78,332 | Bulk in vivo electroporation (single cell) | 4 | dx.doi.org/10.17504/protocols.io.kxygx9qrdg8j/v1 | https://www.protocols.io/view/bulk-in-vivo-electroporation-single-cell-cqq4vvyw | Anya Suppermpool | TITLE: Bulk in vivo electroporation (single cell)
AUTHORS: Anya Suppermpool
[DESCRIPTION]
This protocol will allow you to express single (or near single/mosaic) cell expression in the tectum of larvae. This protocol is adapted from (Hoegler and Horne, 2010; Nikolaou et al., 2015). If you would like to do focal-electro... | ["[Equipments] Stimulator SD9 (Grass Instruments) - borrow from Isaac Bianco (Ask Isaac before use!)", "[Equipments] Electroporation penTM", "[Equipments] Micro glass needle (0.58mm inside diameter, Sutter Instrument, Germany, BF100-58-15) pulled using a micropipette puller (Model P-87 Sutter Instrument, Germany) – nor... |
66,022 | TruKeto Reviews| Scam Or Legit | 1 | dx.doi.org/10.17504/protocols.io.x54v9y4zqg3e/v1 | https://www.protocols.io/view/truketo-reviews-scam-or-legit-ccqesvte | truketoosm | TITLE: TruKeto Reviews| Scam Or Legit
AUTHORS: truketoosm
[DESCRIPTION]
In the current society, different sorts of diets have showed up. Some follow depending on social orders for results, while others accept that obstructing explicit macronutrients takes care of business. This is where the ketogenic diet turns in... | [] |
63,434 | Steve Harvey CBD Gummies THE MOST POPULAR CBD GUMMY BEARS IN UNITED STATES READ HERE REVIEWS, BENEFITS, SIDE EFFECT, INGREDIENTS, DOES IT REALLY WORK? IS IT SAFE? BUY NOW GET INSTANTLY | 1 | dx.doi.org/10.17504/protocols.io.6qpvr62dovmk/v1 | https://www.protocols.io/view/steve-harvey-cbd-gummies-the-most-popular-cbd-gumm-b97ir9ke | Steve Harvey CBD Gummies | TITLE: Steve Harvey CBD Gummies THE MOST POPULAR CBD GUMMY BEARS IN UNITED STATES READ HERE REVIEWS, BENEFITS, SIDE EFFECT, INGREDIENTS, DOES IT REALLY WORK? IS IT SAFE? BUY NOW GET INSTANTLY
AUTHORS: Steve Harvey CBD Gummies
[DESCRIPTION]
Steve Harvey CBD Gummies
Steve Harvey CBD Gummies - You plan to recuperate jus... | ["Steve Harvey CBD Gummies\n\n➢Product Name —Steve Harvey CBD Gummies Reviews\n➢Main Benefits —Improve Metabolism & Help in Pain Relief\n➢ Composition —NaturalOrganic Compound\n➢ Side-Effects —NA\n➢ Rating : —⭐⭐⭐⭐⭐\n➢ Availability —Online\n➢Price (for Sale) Buy Now Here —Click HereSteve Harvey CBD Gu... |
107,619 | Quick estimation of molecular weight from gel images with an ImageJ macro, MolecularWeightEstimator | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qrk4l1y/v1 | https://www.protocols.io/view/quick-estimation-of-molecular-weight-from-gel-imag-dmcb42sn | Kenji Ohgane | TITLE: Quick estimation of molecular weight from gel images with an ImageJ macro, MolecularWeightEstimator
AUTHORS: Kenji Ohgane
[DESCRIPTION]
This protocol shares an ImageJ macro that enables easy and quick estimation of molecular weights from gel electrophoresis images, including the agarose gel electrophoresis for ... | ["[Installation of MolecularWeightEstimator macro into ImageJ] Download the macro file (\"_MolecularWeightEstimator.ijm\") anywhere in your local disk.", "[Installation of MolecularWeightEstimator macro into ImageJ] Start ImageJ.", "[Installation of MolecularWeightEstimator macro into ImageJ] From the menu bar, select ... |
50,790 | Mouse Surgery – Transplantation of human thymus into the kidney capsule of NSG mice | 4 | dx.doi.org/10.17504/protocols.io.bvuen6te | https://www.protocols.io/view/mouse-surgery-transplantation-of-human-thymus-into-bvuen6te | Austin Chen, Mohsen Khosravi-Maharlooei, Markus Holzl, Megan Sykes | TITLE: Mouse Surgery – Transplantation of human thymus into the kidney capsule of NSG mice
AUTHORS: Austin Chen, Mohsen Khosravi-Maharlooei, Markus Holzl, Megan Sykes
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details our minimally invasive approach for implanting human thymus ti... | ["[Day 0 or 1, Irradiation]\n1. Put the mice into an irradiation cage2. Irradiate the mouse with indicated grey 120 – 150 for NSG 250-270 for NS3. Put the mice back into their original cage", "[Day 1, Thymus surgery (all of these steps take place within the hood)]\nMake sure to read and understand steps 14-19 pr... |
62,611 | Oprah Winfrey Keto Pills Reviews - Where To Buy It? | 1 | dx.doi.org/10.17504/protocols.io.n92ldz26xv5b/v1 | https://www.protocols.io/view/oprah-winfrey-keto-pills-reviews-where-to-buy-it-b9dtr26n | oprahwinfreypillsreviews | TITLE: Oprah Winfrey Keto Pills Reviews - Where To Buy It?
AUTHORS: oprahwinfreypillsreviews
[DESCRIPTION]
Oprah Winfrey Keto Pills Reviews - Where To Buy It?
[STEPS]
1. Oprah Winfrey Keto Pills Reviews - Where To Buy It?
Oprah Winfrey Keto Reviews-If you have decided that the time has come to lose a many redundan... | ["Oprah Winfrey Keto Pills Reviews - Where To Buy It?\nOprah Winfrey Keto Reviews-If you have decided that the time has come to lose a many redundant pounds, know that the path to take is neither easy nor short.\nStill, presumably, with the right provocation, If it were enough to suffer exhausting sessions in the spa o... |
null | null | null | dx.doi.org/10.17504/protocols.io.grxbv7n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 1">
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<p><strong>Developed for:</strong></p>
<p> </p>
<p>Aerius,<br /> Odyssey® Classic,</p>
<p>Odyssey CLx, and</p>
<p>Odyssey Sa<br /> Infrared Imaging Systems</p>
</div>
</div>
</div>
[GUIDELINES]
<p><... | [] |
96,841 | FUNDIS ONT Post-PCR Pooling & Purification for Fungal Barcoding | 4 | null | https://www.protocols.io/view/fundis-ont-post-pcr-pooling-amp-purification-for-f-dath2ej6 | Stephen Douglas Russell, Harte Singer | TITLE: FUNDIS ONT Post-PCR Pooling & Purification for Fungal Barcoding
AUTHORS: Stephen Douglas Russell, Harte Singer
[DESCRIPTION]
Pooling PCR products and purifying them.
[STEPS]
SECTION: Preparation
1. Bring magnetic beads to room temp. **Very Important**
SECTION: Preparation
2. Heat a fresh, sterile 1.5uL t... | ["[Preparation] Bring magnetic beads to room temp. **Very Important**", "[Preparation] Heat a fresh, sterile 1.5uL tube of molecular grade water or Low TE buffer to 55 °C in the heat block. 500uL should be sufficient.", "[Preparation] Create a fresh batch of 80% ethanol. You will be using 2 mL in this protocol. You wil... |
null | null | null | dx.doi.org/10.17504/protocols.io.t2ieqce | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This in silico protocol allows to have an insight into the sequence divergence of selected genomic region, including missing information, gaps and allignment with Open Reading Frames. The protocol is written for model plant Arabidopsis thaliana, but can be possibly adapted to pl... | ["{\"blocks\":[{\"key\":\"dle4v\",\"text\":\"Download the sequences of the group of accessions you want to compare from 1001 genomes SALK Genome Browser DB and save them in text-editor (make sure to remove all \\u201c[\\u201c, \\u201c]\\u201d, and \\u201c-\\u201c. Dots representing the missing data have to stay in the ... |
null | null | null | dx.doi.org/10.17504/protocols.io.n9zdh76 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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69,030 | Enhancing the growth of coral microfragments using the 'Green Film' Husbandry Method | 4 | dx.doi.org/10.17504/protocols.io.261genqmdg47/v1 | https://www.protocols.io/view/enhancing-the-growth-of-coral-microfragments-using-cfnetmbe | Christopher Page, Riley Perry, Claire Lager, E. Michael Henley, Mary Hagedorn | TITLE: Enhancing the growth of coral microfragments using the 'Green Film' Husbandry Method
AUTHORS: Christopher Page, Riley Perry, Claire Lager, E. Michael Henley, Mary Hagedorn
[DESCRIPTION]
The purpose of this method is to produce a repeatable husbandry protocol that increases coral microfragment growth, by... | ["[Establishing 'Green Film' on tank surfaces] Scrub, acid wash, and rinse clean the intended vessel prior to startup", "[Establishing 'Green Film' on tank surfaces] Equip the tank with two cleaned and sanitized air stones, an overflow strainer, and PVC seawater outfall", "[Establishing 'Green ... |
47,397 | Effective asexual propagation (fragmentation) method of the soft coral Xenia umbellata | 4 | dx.doi.org/10.17504/protocols.io.bsidnca6 | https://www.protocols.io/view/effective-asexual-propagation-fragmentation-method-bsidnca6 | Sohyoung Kim, Christian Wild, Arjen Tilstra | TITLE: Effective asexual propagation (fragmentation) method of the soft coral Xenia umbellata
AUTHORS: Sohyoung Kim, Christian Wild, Arjen Tilstra
[DESCRIPTION]
Many asexual propagation methods for hard corals can easily be found in scientific papers, while methods for soft corals are mostly present in grey literatu... | ["Select a coral stock and prepare it in a water basin filled with identical water from where it comes from.", "Cut prepared stock into the desired size with scissors or a scalpel. Use tweezers if needed to secure them and always keep them in the water.", "Gently allocate them on top of plugs inside eggcrate grids.", "... |
null | null | null | dx.doi.org/10.17504/protocols.io.mpcc5iw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
20,317 | Transfectionof constructs in diplonemids to block NHEJ pathway using the inhibitors of KU70/80 proteins. | null | dx.doi.org/10.17504/protocols.io.x35fqq6 | null | Binnypreet Kaur1, 2, Drahomíra Faktorová1, 2 and Julius Lukeš1, 2, 3 | TITLE: Transfectionof constructs in diplonemids to block NHEJ pathway using the inhibitors of KU70/80 proteins.
AUTHORS: Binnypreet Kaur1, 2, Drahomíra Faktorová1, 2 and Julius Lukeš1, 2, 3
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The process of NHEJ is completed when the DNA ligase complex ... | ["Step 0: Pre-incubation of Diplonema cells for 4 hours with W7 (5 mg/ml) beforeelectroporation. EC value of W7 was calculated by Alamar blue assay", "Count the cells and plan to 1 nucleofection with 5x 107 cells for each construct.", "Resuspendthe cell pellet in 100ul of AMAXA Human T- cell solution at 4C (from refri... |
20,179 | Wheat Protoplast Preparation and Transformation | 1 | dx.doi.org/10.17504/protocols.io.q26g7r3zkvwz/v1 | https://www.protocols.io/view/wheat-protoplast-preparation-and-transformation-xxtfpnn | Salome Wilson, Bayantes Dagvadorj, Rita Tam, Benjamin Schwessinger | TITLE: Wheat Protoplast Preparation and Transformation
AUTHORS: Salome Wilson, Bayantes Dagvadorj, Rita Tam, Benjamin Schwessinger
[DESCRIPTION]
Wheat protoplast protocol for studying effector recognition and cell-autonomous defense signaling. Plant resistance (R) proteins recognize transiently expressed Avirulence (... | ["[During incubation] Prepare PEG-Ca solution (shake vigorously to dissolve PEG) Make freah each day, don't reuse\n \n\nDispense amount of W5 and PEG required for transfection according to setup\nThaw 5% BSA and label culture plates for incubation", "[Washing] Add 5 mL of W5 solution to petri dish\nAfter three hours, r... |
43,652 | Primers for bark beetles and associated fungi | 3 | dx.doi.org/10.17504/protocols.io.bnvcme2w | https://www.protocols.io/view/primers-for-bark-beetles-and-associated-fungi-bnvcme2w | Andrew J. Johnson, Demian F Gomez, Jiri Hulcr | TITLE: Primers for bark beetles and associated fungi
AUTHORS: Andrew J. Johnson, Demian F Gomez, Jiri Hulcr
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the primers used for bark beetles and associated fungi.</div><div class = "text-block"><span>This protocol is part of th... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.n6sdhee | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The microbiological testing method for Salmonella spp. as used for testing primary envrionmental samples collected from poultry environments</p>
<p>The method is described as detailed in accordance with the Australian Standard 5013.10-2009 Horizontal method for the detection ... | [] |
87,531 | Eastern Hemlock Tissue Collection for DNA | 4 | null | https://www.protocols.io/view/eastern-hemlock-tissue-collection-for-dna-czqjx5un | Karl Fetter | TITLE: Eastern Hemlock Tissue Collection for DNA
AUTHORS: Karl Fetter
[DESCRIPTION]
Steps for collecting tissue from Eastern Hemlocks for DNA analysis. The Plant Computational Genomics lab is conducting a landscape genomics study of climate adaptation in the Eastern Hemlock. This protocol is a step-by-step guide to co... | ["[Introduction] This protocol is intended for field collection of leaf tissue for the Eastern Hemlock conservation genomics project sponsored by the Plant Computation Genomics lab at the University of Connecticut. The goal of the project is to identify climate adapted genomic variation for seed banking and potential u... |
67,320 | Microplastics in marine environments: protocol for isolating natural biofilms from seawater-incubated particles | 4 | dx.doi.org/10.17504/protocols.io.kqdg3pzjel25/v1 | https://www.protocols.io/view/microplastics-in-marine-environments-protocol-for-cdyys7xw | Meril Massot, elsa gadoin, Andreja Rajkovic, stephanie.bedhomme | TITLE: Microplastics in marine environments: protocol for isolating natural biofilms from seawater-incubated particles
AUTHORS: Meril Massot, elsa gadoin, Andreja Rajkovic, stephanie.bedhomme
[DESCRIPTION]
Microplastics, upon which microbial communities arise rapidly, have emerged as a significant source of pollution ... | ["[SAMPLE PREPARATION] MICROPLASTIC PARTICLES CONDITIONING\nMicroplastics are enclosed in nylon mesh bags with a mesh size inferior to microparticule size to ensure their entrapment. Each bag will contain one type of plastic polymer. Three replicates per polymer and per site should be conducted.", "[ON-FIELD SETUP] INC... |
51,403 | Relative quantification of mRNA transcript levels by qPCR | 1 | dx.doi.org/10.17504/protocols.io.14egnz12qg5d/v1 | https://www.protocols.io/view/relative-quantification-of-mrna-transcript-levels-bwfjpbkn | Will Hancock-Cerutti, Zheng Wu, Gerald S. Shadel, Pietro De Camilli | TITLE: Relative quantification of mRNA transcript levels by qPCR
AUTHORS: Will Hancock-Cerutti, Zheng Wu, Gerald S. Shadel, Pietro De Camilli
[DESCRIPTION]
This method describes isolation of RNA from cultured cells, generation of cDNA, and relative quantification of transcript levels by qPCR.
[STEPS]
SECTION: Cell cu... | ["[Cell culture and treatments] Culture the HeLa-M cells at 37 °C in 5% CO2 and DMEM containing 10% FBS, 100 U/ml penicillin, 100 mg/mL streptomycin, and 2 Milimolar (mM) L-glutamine (all from Gibco).", "[Cell culture and treatments] For any given experiment, plate the cells at such density so as to be approximately 90... |
29,498 | Brain Clearing (iDISCO) | 1 | null | https://www.protocols.io/view/brain-clearing-idisco-822hyge | Adam Kimbrough, Olivier George | TITLE: Brain Clearing (iDISCO)
AUTHORS: Adam Kimbrough, Olivier George
[STEPS]
?. Day 1: See materials for solutions to mix:Dehydrate brains with methanol H2O series 1h each wash increasing in concentration (20%, 40%, 60%, 80%, 100%) and once again with 100% (6h total)Incubate brains in 66% dichloromethane (DCM)/33% m... | ["Day 1: See materials for solutions to mix:Dehydrate brains with methanol H2O series 1h each wash increasing in concentration (20%, 40%, 60%, 80%, 100%) and once again with 100% (6h total)Incubate brains in 66% dichloromethane (DCM)/33% methanol overnightDay 2:Wash the brains twice for 1h each with 100% methanol to ri... |
22,011 | Protocols involving the pgMAX plasmid | null | dx.doi.org/10.17504/protocols.io.zq3f5yn | null | manabu murakami | TITLE: Protocols involving the pgMAX plasmid
AUTHORS: manabu murakami
[STEPS]
?. Protocols involving the pgMAX plasmidManabu Murakami Department of Pharmacology, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562, Japan. Corresponding to: mmura0123@hotmail.co.jp [Abstract]The pgMAX system involves dir... | ["Protocols involving the pgMAX plasmidManabu Murakami Department of Pharmacology, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562, Japan. Corresponding to: mmura0123@hotmail.co.jp [Abstract]The pgMAX system involves direct subcloning of a DNA fragment into a prokaryotic DNA expression plasmid and r... |
28,383 | MojoSort™ Isolation Kits Protocol - 3 | null | dx.doi.org/10.17504/protocols.io.7x7hprn | null | Sam Li | TITLE: MojoSort™ Isolation Kits Protocol - 3
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Product description and procedure summary:</span></div><div class = "text-block"><span>Target cells are depleted by incubating your sample with the biotin an... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes. Kits for human samples have been optimized for PBMCs, please prepare the cells using a suitable method.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4mL in a 5 mL (12 x 75 mm) p... |
23,826 | hbes | null | dx.doi.org/10.17504/protocols.io.3hsgj6e | null | dikei@hanmail.net smc1710802 | TITLE: hbes
AUTHORS: dikei@hanmail.net smc1710802
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Original data-set of the sutdy entitled " risk factors associated with hypotensive bradycardic events during open shoulder surgery in the beach chair position'</div></div>
[STEPS] | [] |
75,766 | Testing Framework Protocol | 1 | dx.doi.org/10.17504/protocols.io.36wgqjmbxvk5/v1 | https://www.protocols.io/view/testing-framework-protocol-cm8wu9xe | Alex Lepauvre, Rony Hirschorn, Lucia Melloni, mudrikli | TITLE: Testing Framework Protocol
AUTHORS: Alex Lepauvre, Rony Hirschorn, Lucia Melloni, mudrikli
[DESCRIPTION]
This protocol provides a step-by-step guide for testing and validating experimental setups in visual event-related research. It includes Testing Preparation required to conduct the protocol, Visual Angle Che... | ["[Controlled Event content logging] Number of tested events conditions", "[Controlled Event content logging] Describe method used to test:", "[Controlled Event content logging] Number of tested events per condition:", "[Controlled event timing logging] Describe method used to test:", "[Controlled event timing logging]... |
37,486 | Thawing, Expanding, and Freezing Human Fibroblasts | null | dx.doi.org/10.17504/protocols.io.bgunjwve | https://www.protocols.io/view/thawing-expanding-and-freezing-human-fibroblasts-bgunjwve | Jacob Marsh, Rj Martinez, Celeste Karch | TITLE: Thawing, Expanding, and Freezing Human Fibroblasts
AUTHORS: Jacob Marsh, Rj Martinez, Celeste Karch
[STEPS]
?. [Thawing Human Fibroblasts]
Prior to thawing cells, prepare culture media
?. [Thawing Human Fibroblasts]
hFibroblast Media: DMEM/F12, 10-20%FBS1% L-Glut (or Glutamax)1% Antibiotic
?. [Thawing Human Fib... | ["[Thawing Human Fibroblasts]\nPrior to thawing cells, prepare culture media", "[Thawing Human Fibroblasts]\nhFibroblast Media: DMEM/F12, 10-20%FBS1% L-Glut (or Glutamax)1% Antibiotic", "[Thawing Human Fibroblasts]\nRemove cells from liquid nitrogen storage and thaw quickly by placing vial in a water bath for approxim... |
null | null | null | dx.doi.org/10.17504/protocols.io.mcyc2xw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Short DNA probes, for example, those used in characterizing protein-DNA complexes, have historically been radiolabeled with <sup>32</sup>P to allow their detection and quantitation following native polyacrylamide gel electrophoresis (PAGE). For reasons of economy or safety, a... | [] |
17,944 | Collect of Amoebophrya parasite (free-living stage) for genomic and transcriptomic analyses | null | dx.doi.org/10.17504/protocols.io.vrye57w | null | Estelle Bigeard | TITLE: Collect of Amoebophrya parasite (free-living stage) for genomic and transcriptomic analyses
AUTHORS: Estelle Bigeard
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><span style = "font-style:italic;">Amoebophrya</span><span> (Syndiniales or ... | ["Host and parasite culturesMediaInfected and uninfected host cultures were maintained in F/2 medium (Guillard’s Marine Water Enrichment Solution, Sigma), using filtered and autoclaved natural seawater from the Penzé estuary collected in June one year before at the 27 of salinity and stored at dark. After preparation, ... |
99,913 | Subcutaneous Injection Adult Mouse | 1 | dx.doi.org/10.17504/protocols.io.3byl47ryolo5/v2 | https://www.protocols.io/view/subcutaneous-injection-adult-mouse-ddth26j6 | Allen Institute for Brain Science | TITLE: Subcutaneous Injection Adult Mouse
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
This protocol describes general procedures used for subcutaneous injection in adult mice.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes o... | [] |
39,384 | Donor Acceptance Criteria for TMC Florida/Zurich HuBMAP Inclusion | 1 | dx.doi.org/10.17504/protocols.io.bipykdpw | https://www.protocols.io/view/donor-acceptance-criteria-for-tmc-florida-zurich-h-bipykdpw | Marda Jorgensen | TITLE: Donor Acceptance Criteria for TMC Florida/Zurich HuBMAP Inclusion
AUTHORS: Marda Jorgensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This document outlines the required criteria for inclusion for donors of thymus, lymph nodes and spleen in the HuBMAP project.</div></div>
[STEPS]
?. [Do... | ["[Donor Criteria]\nDonor must be less than seven days on ventilator", "[Donor Criteria]\nDonor tissue must be received within 24 hours of recovery", "[Donor Criteria]\nContraindications for donor acceptance are", "[Donor Criteria]\nMore than 4 blood transfusions", "[Donor Criteria]\nAny autoimmune diseases", "[Donor C... |
103,373 | BAF_S07_SpeedVac SPD1030 | 0 | dx.doi.org/10.17504/protocols.io.j8nlk8mmwl5r/v1 | https://www.protocols.io/view/baf-s07-speedvac-spd1030-dg7m3zk6 | Nicholas Sherman | TITLE: BAF_S07_SpeedVac SPD1030
AUTHORS: Nicholas Sherman
[DESCRIPTION]
General use of the SpeedVac for drying samples that contain aqueous or organic solvents. This is room temp and does not keep samples frozen like lyophilization.
[STEPS]
SECTION: SpeedVac SPD1030 ThermoScientific
1. First start day:
--> Ensure tha... | ["[SpeedVac SPD1030 ThermoScientific] First start day:\n--> Ensure that the refrigerated trap contains a clean, dry, Glass Condensation Flask (GCF400) and that the supply of Thermo Scientific CryoCoolTM (SCC1) Heat Transfer Fluid is sufficient.\nThe CryoCoolTM in the refrigerated trap must be cold before drying runs ca... |
49,602 | ADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes | 2 | dx.doi.org/10.17504/protocols.io.bupanvie | https://www.protocols.io/view/adi-isletcore-protocols-for-the-isolation-assessme-bupanvie | James Lyon, Aliya Spigelman, Jocelyn E Manning Fox, Patrick Macdonald | TITLE: ADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes
AUTHORS: James Lyon, Aliya Spigelman, Jocelyn E Manning Fox, Patrick Macdonald
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This collection of pro... | [] |
26,934 | FLASH v2.0 | null | dx.doi.org/10.17504/protocols.io.6iwhcfe | null | Amy Lyden, Gloria Castañeda, Emily Crawford, Saharai Caldera, Jenai Quan, Katherine Travisano | TITLE: FLASH v2.0
AUTHORS: Amy Lyden, Gloria Castañeda, Emily Crawford, Saharai Caldera, Jenai Quan, Katherine Travisano
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> FLASH is a crispr-... | ["[Dephosphorylation]\nPrepare a reaction for each cDNA or gDNA sample and mix well. Add the components in the order specified below. You can make a master mix (MM) of the rapid alkaline phosphatase (RAP) buffer and enzyme. If you are using the RAP MM, mix of MM with each sample. Mix thoroughly with a pipette or by ta... |
31,409 | AAV Titration by qPCR Using SYBR Green Technology | null | dx.doi.org/10.17504/protocols.io.bawrifd6 | null | Addgene the nonprofit plasmid repository | TITLE: AAV Titration by qPCR Using SYBR Green Technology
AUTHORS: Addgene the nonprofit plasmid repository
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol goes through AAV titration by qPCR using SYBR Green Technology. To see the full abstract and additional resources, visit the ... | ["[Procedure]\nPrepare a plasmid stock of 2x109molecules/μl to generate a standard curve:To obtain a solution at 2x109 molecules/μL:1.59 x 1011 / 2x109 = 79.8x dilution 100 μL / 79.8 = Therefore we need to dilute stock into H20\nOne option is to use plasmid #59462 from Addgene. The values highlighted below in red wer... |
68,718 | HTTM : Transposon mutagenesis | 4 | dx.doi.org/10.17504/protocols.io.36wgq72n3vk5/v2 | https://www.protocols.io/view/httm-transposon-mutagenesis-cfcntive | Antoine Champie | TITLE: HTTM : Transposon mutagenesis
AUTHORS: Antoine Champie
[DESCRIPTION]
Part of the HTTM protocol dedicated to the transposon mutagenesis of targets cells.
[BEFORE_START]
Per plate refers to the number of 96 well plates of target cells that need to be processed.
[STEPS]
SECTION: Day 1
1. (1-A) Make a 15 mL LB ... | ["[Day 1] (1-A) Make a 15 mL LB (Diaminopimelic acid [Dap], Ampicillin [Amp], Spectinomycin [Spec]) pre-culture ( 2 mL per plate minimum) of the donor strain eAC494 and incubate with agitation at 37 °C overnight.", "[Day 1] (1-B) Prepare the 96 deep-well plates for conjugation :", "[Day 1] Preheat the deep-well plates ... |
96,315 | Illustrated Protocol for Processing Radiometric Videos Using the Software FLIR Tools : An Approach Applied to a Novel Methodology for Lizard Thermal Exchange Rates Studies | 0 | dx.doi.org/10.17504/protocols.io.n2bvj358xlk5/v1 | https://www.protocols.io/view/illustrated-protocol-for-processing-radiometric-vi-daa32agn | Frederico M Barroso | TITLE: Illustrated Protocol for Processing Radiometric Videos Using the Software FLIR Tools : An Approach Applied to a Novel Methodology for Lizard Thermal Exchange Rates Studies
AUTHORS: Frederico M Barroso
[DESCRIPTION]
The novel methodology developed by Mochales-Riaño & Barroso et al. (in press) for experimentally ... | [] |
51,474 | Effect of immunonutrition on serum levels of C-Reactive Protein and lymphocytes in patients with COVID-19: Randomized controlled double-blind clinical trial | 3 | dx.doi.org/10.17504/protocols.io.bwhspb6e | https://www.protocols.io/view/effect-of-immunonutrition-on-serum-levels-of-c-rea-bwhspb6e | Rodrigo Fernandes Weyll Pimentel, Magno Conceição das Merces | TITLE: Effect of immunonutrition on serum levels of C-Reactive Protein and lymphocytes in patients with COVID-19: Randomized controlled double-blind clinical trial
AUTHORS: Rodrigo Fernandes Weyll Pimentel, Magno Conceição das Merces
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "just... | [] |
81,364 | 10x Protocols: Visium v1 FFPE Deparaffinization, H&E staining, and Imaging -- University of Minnesota TMCs (CG000409 Rev B) | 1 | dx.doi.org/10.17504/protocols.io.5qpvorm3bv4o/v1 | https://www.protocols.io/view/10x-protocols-visium-v1-ffpe-deparaffinization-h-a-ctpuwmnw | IOx Genomics, Laura J Niedernhofer, David A Bernlohr | TITLE: 10x Protocols: Visium v1 FFPE Deparaffinization, H&E staining, and Imaging -- University of Minnesota TMCs (CG000409 Rev B)
AUTHORS: IOx Genomics, Laura J Niedernhofer, David A Bernlohr
[DESCRIPTION]
Protocols from 10x Genomics for Visium Spatial Gene Expression v1 chemistry on FPPE samples without the Cyt... | ["Additional Protocols/Resources\n \n \nhttps://www.10xgenomics.com/support/spatial-gene-expression-ffpe", "10x protocol CG000409, Rev B (Deparaffinization, H&E, etc)"] |
null | null | null | dx.doi.org/10.17504/protocols.io.e4hbgt6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>SpinOUT™ protocol for desalting of peptide and protein samples.</p>
<p>(Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)</p>
[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>The SpinOUT™ columns are versatile, spin-format columns for the desalting and b... | [] |
28,450 | MojoSort™ Mouse CD326 (Ep-CAM) Positive Selection Protocol | null | dx.doi.org/10.17504/protocols.io.72ahqae | null | Sam Li | TITLE: MojoSort™ Mouse CD326 (Ep-CAM) Positive Selection Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Mouse CD326 (Ep-CAM) cells are selected by incubating the sample with biotin conjugated anti-mouse CD326 antibody after blocking with TruStain FcX™ (anti-mouse CD16/32 an... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes. Kits for human samples have been optimized for PBMCs, please prepare the cells using a suitable method.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4mL in a 5 mL (12 x 75 mm) p... |
86,053 | Detection of arboviruses in salivary glands and midgut of mosquitoes vector | 1 | dx.doi.org/10.17504/protocols.io.8epv5xjndg1b/v1 | https://www.protocols.io/view/detection-of-arboviruses-in-salivary-glands-and-mi-cyadxsa6 | ISABELLA FERREIRA DA COSTA, CAROLINE PIRES SANTANA, Isabela Cinquini Junqueira, Juscelino Rodrigues, Christian Luz, Suleimy Marinho Fernandes, Izabela Batista Melo, Fabíola Fiaccadori, Valéria Christina de Rezende Feres | TITLE: Detection of arboviruses in salivary glands and midgut of mosquitoes vector
AUTHORS: ISABELLA FERREIRA DA COSTA, CAROLINE PIRES SANTANA, Isabela Cinquini Junqueira, Juscelino Rodrigues, Christian Luz, Suleimy Marinho Fernandes, Izabela Batista Melo, Fabíola Fiaccadori, Valéria Christina de Rezende Feres
[DESCR... | ["[Before starting] Prepare pools of 2 to 10 live and engorged an non-engorged adult mosquito specimens, sorted by sex (male/female). Place the pools in 1.5 µl microtubes and store in a freezer at -80°C.", "[Mosquitoes dissection] Pinch one mosquito at a time and remove the legs.", "[Mosquitoes dissection] Place the mo... |
27,896 | Human Immunodeficiency Virus 1+2 (HIV) Antibody (HIV(1+2)-Ab) ELISA Kit by Abbexa | null | dx.doi.org/10.17504/protocols.io.7gyhjxw | null | Devi Oktafiani | TITLE: Human Immunodeficiency Virus 1+2 (HIV) Antibody (HIV(1+2)-Ab) ELISA Kit by Abbexa
AUTHORS: Devi Oktafiani
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over</div><div class = "tex... | ["Sample -\tWhole blood from plain tube (red cap) collection 3 mL -\tCentrifuge at approximately 1000 × g for 15 min.", "Wash buffer Dilute the concentrated Wash buffer 20-fold (1/20) with distilled water (i.e. add 25 ml of concentrated wash buffer into 475 ml of distilled water). B. Assay Procedure Equilibrate the ki... |
45,216 | Minimally-invasive sampling of pars petrosa (os temporale) for ancient DNA extraction | 1 | dx.doi.org/10.17504/protocols.io.bqd8ms9w | https://www.protocols.io/view/minimally-invasive-sampling-of-pars-petrosa-os-tem-bqd8ms9w | Eleftheria Orfanou, Marie Himmel, Franziska Aron, Wolfgang Haak | TITLE: Minimally-invasive sampling of pars petrosa (os temporale) for ancient DNA extraction
AUTHORS: Eleftheria Orfanou, Marie Himmel, Franziska Aron, Wolfgang Haak
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes how to obtain bone powder from the </span><span style = ... | ["[Workstation preparation]\nPlace a sheet of aluminium foil under the hood.Place weighing paper, drill bits, and forceps on a sheet of aluminum foil on an easily accessible clean surface outside of the hood. Alternatively, you may place them within hood but be sure to cover them with aluminum foil or a paper towel to ... |
67,386 | Huuman CBD Gummies | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkrx7vmk/v1 | https://www.protocols.io/view/huuman-cbd-gummies-cd22s8ge | Huuman CBD Gummies | TITLE: Huuman CBD Gummies
AUTHORS: Huuman CBD Gummies
[DESCRIPTION]
Huuman CBD Gummies
[STEPS]
1. Huuman CBD Gummies Reviews - Trending 100% Pure Easy Solution For Pain Relief! Cost & Website
2. Huuman CBD Gummies are supplemented and further developed that could mitigate any optional impacts connected with physical ... | ["Huuman CBD Gummies Reviews - Trending 100% Pure Easy Solution For Pain Relief! Cost & Website", "Huuman CBD Gummies are supplemented and further developed that could mitigate any optional impacts connected with physical and mental agony. Making this CBD supplement in the Huuman CBD Gummies is valuable, as it allows a... |
38,747 | Analysis of BDNF protein expression | 1 | dx.doi.org/10.17504/protocols.io.bh33j8qn | https://www.protocols.io/view/analysis-of-bdnf-protein-expression-bh33j8qn | Tamiris Silva, Sandra Kalil Bussadori, Raquel Agnelli Mesquita-Ferrari | TITLE: Analysis of BDNF protein expression
AUTHORS: Tamiris Silva, Sandra Kalil Bussadori, Raquel Agnelli Mesquita-Ferrari
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Neuroplasticity regards physiological and/or structural changes in circuits in response to changes in patterns caused by an injur... | [] |
54,351 | Experiment 1 | 1 | null | https://www.protocols.io/view/experiment-1-bzbpp2mn | Dikla Perez , Yael Steinhart , Amir Grinstein , Meike Morren | TITLE: Experiment 1
AUTHORS: Dikla Perez , Yael Steinhart , Amir Grinstein , Meike Morren
[DESCRIPTION]
In this experiment, we aimed to provide evidence regarding the effect of expected product visibility on consumers’ likelihood of engaging in consistent behavior when they are faced with two sequential choices in th... | ["Experimental design:\n We have employed two expected-visibility conditions (high vs. low) in a between-subjects design.", "Sample\nn = 179 residents of major cities (64.8% women, Mage = 29.22, SDage = 4.45) were paid to take part in an online experiment. Participants were each randomly assigned to one of two expected... |
null | null | null | dx.doi.org/10.17504/protocols.io.gr6bv9e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The SpinSmart PCR purification and gel extraction technologies utilize a lysis buffer containing chaotropic salts that allow DNA to bind to a silica membrane. Binding buffer PCR 1 is added to a PCR reaction or agarose gel slice; the mixture is subsequently loaded directly ont... | [] |
103,755 | An ImageJ macro for the quantification of ciliary accessible cholesterol | 0 | dx.doi.org/10.17504/protocols.io.q26g71d88gwz/v1 | https://www.protocols.io/view/an-imagej-macro-for-the-quantification-of-ciliary-dhjj34kn | Sreeja V Nair, Jonas Nikoloff, Suzanne R Pfeffer | TITLE: An ImageJ macro for the quantification of ciliary accessible cholesterol
AUTHORS: Sreeja V Nair, Jonas Nikoloff, Suzanne R Pfeffer
[DESCRIPTION]
ALOD4, a toxin-based probe, can be used to visualize the accessible pool of cholesterol (1). Here we describe an algorithm for segmenting fluorescently-labeled cilia u... | ["[Description of the Macro: Four basic steps] Step 1: Drag and drop the macro to ImageJ/Fiji", "[Description of the Macro: Four basic steps] Step 2: Click “Run’’. The macro requests the user for a folder containing .tif files of images to be analyzed. Choose the folder, click ‘Open’.", "[Description of the Macro: Four... |
101,406 | OT-2 Protocol to transfer volume from several plates to a single plate | 5 | dx.doi.org/10.17504/protocols.io.6qpvr4o62gmk/v3 | https://www.protocols.io/view/ot-2-protocol-to-transfer-volume-from-several-plat-de963h9e | Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno | TITLE: OT-2 Protocol to transfer volume from several plates to a single plate
AUTHORS: Ana Mariya Anhel, Lorea Alejaldre, Ángel Goñi-Moreno
[DESCRIPTION]
This protocol is meant to transfer samples from different plates to a single or fewer final plates than the source number, in other words, merge samples of differe... | ["[Files Preparation] Preparing Customized Template\n\nPreparing the template (a .xlsx) with the specific variables for each experiment.\n\nAttached there is a template of the variable file with several sheets and a PDF file explaining each variable:\nGeneralVariables: variables related to the labware to be used\nPipet... |
103,602 | Agrobacterium-Mediated Transient Expression in Nicotiana benthamiana Leaves | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj7prlx1/v1 | https://www.protocols.io/view/agrobacterium-mediated-transient-expression-in-nic-dhes33ee | Maryam Rahmati Ishka | TITLE: Agrobacterium-Mediated Transient Expression in Nicotiana benthamiana Leaves
AUTHORS: Maryam Rahmati Ishka
[DESCRIPTION]
This procedure outlines the Agrobacterium infiltration of tobacco leaves for the transient expression of fluorescently labeled proteins.
[BEFORE_START]
n/a
[GUIDELINES]
n/a
[STEPS]
SECTION:... | ["[Transform constructs into Agrobacterium tumefaciens strains that are compatible with vectors. Strain GV3101 is typically used (GV2260 can also be used). Make glycerol stock of.] Refresh bacteria containing the construct of interest from glycerol stock on agar YEP (or any other bacterial media such as LB) medium with... |
47,398 | Antibiotics for algaculture | 4 | null | https://www.protocols.io/view/antibiotics-for-algaculture-bsiencbe | Victoria Jackson | TITLE: Antibiotics for algaculture
AUTHORS: Victoria Jackson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Antibiotics recipe adapted from Roscoff Culture Collection protocol.</div></div>
[STEPS]
?. [ABX solution]
To of , add any 3 of:
10 mL
[Ampicillin]
[Gentamicin]
[Kanamycin]
[Neomycin]
[Stre... | ["[ABX solution]\nTo of , add any 3 of:\n10 mL\n[Ampicillin]\n[Gentamicin]\n[Kanamycin]\n[Neomycin]\n[Streptomycin]\nIt is best not to use all ABX in every solution, but to use a selection of 2-3 so that you can change the combinations in future solutions in case of bacterial resistance developing.", "[ABX solution]\n... |
null | null | null | dx.doi.org/10.17504/protocols.io.fftbjnn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
53,712 | Reprogramming fibroblasts and mononuclear blood cells into iPSCs using Sendai virus | 1 | dx.doi.org/10.17504/protocols.io.bypqpvmw | https://www.protocols.io/view/reprogramming-fibroblasts-and-mononuclear-blood-ce-bypqpvmw | Dmitry Ovchinnikov | TITLE: Reprogramming fibroblasts and mononuclear blood cells into iPSCs using Sendai virus
AUTHORS: Dmitry Ovchinnikov
[DESCRIPTION]
Reprogramming primary blood mononuclear suspension cells into induced pluripotent stem cells using a non-integrating RNA viral (Sendai) reprogramming factor delivery method. This ™ CytoT... | ["[Preparation of Reagents and Materials] Fibroblast culture medium:\n\nThe following example is for preparing 50 mL of Fibroblast Culture Medium. If preparing other volumes, adjust accordingly.\nCombine the following:\n45 mL of DMEM (+Glutamate) base medium\n5 mL of fetal bovine serum (FBS), ES-qualified\nNEAA 1x (Gib... |
69,710 | Illumina denature and dilute | 4 | dx.doi.org/10.17504/protocols.io.6qpvr4ex2gmk/v1 | https://www.protocols.io/view/illumina-denature-and-dilute-cgbntsme | Angela R.S. Kruse, Morad C Malek, Jamie Allen, Melissa Farrow, Jeff Spraggins | TITLE: Illumina denature and dilute
AUTHORS: Angela R.S. Kruse, Morad C Malek, Jamie Allen, Melissa Farrow, Jeff Spraggins
[DESCRIPTION]
This protocol describes the preparation of a library for Illumina NGS sequencing.
[STEPS]
SECTION: Standard Normalization Method
1. Use the following steps to denature and dilute l... | ["[Standard Normalization Method] Use the following steps to denature and dilute libraries that have been normalized using standard library quantification and quality control procedures recommended in the library prep documentation.\n\nFollow the steps most appropriate for your library and the version of Illumina Reage... |
98,150 | Opentrons Dual-Index Primer Plate Workflow | 1 | dx.doi.org/10.17504/protocols.io.3byl4q66ovo5/v2 | https://www.protocols.io/view/opentrons-dual-index-primer-plate-workflow-db4e2qte | Stephen Douglas Russell | TITLE: Opentrons Dual-Index Primer Plate Workflow
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
This protocol is designed to utilize the Opentrons robotic automation platform to generate dual-index primer - master mix plates for PCR reactions for NGS, particularly for DNA barcoding large specimen pools with Oxford Na... | ["[Initial Prep] Starting Point – 1 Stock Primer Plate with 96 different ONT-tagged primers. At least 90uL fluid at 100uM concentration. Spin down this plate. Transfer liquid to a BioRad 200uM plate. If you have less than 90uL in any of the cells, you will need to make modifications to the programs.", "[OT-2 "Work... |
90,820 | In vitro dephosphorylation | 1 | dx.doi.org/10.17504/protocols.io.kqdg3xb1pg25/v1 | https://www.protocols.io/view/in-vitro-dephosphorylation-c4xcyxiw | Leonardo A Parra-Rivas | TITLE: In vitro dephosphorylation
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
In vitro dephosphorylation
[STEPS]
1. 100 μg of α-syn GST fusion protein on beads were resuspended in alkaline
phosphatase (ALP) buffer supplemented with 250 units of ALP (Thermo Scientific
Cat# EF0651).
2. The mixes were incubated ... | ["100 μg of α-syn GST fusion protein on beads were resuspended in alkaline\nphosphatase (ALP) buffer supplemented with 250 units of ALP (Thermo Scientific\nCat# EF0651).", "The mixes were incubated for 60 min or 180 min at 37 °C with\norbital shaking (Eppendorf ThermoMixer F1.5).", "Samples were then analyzed by\nNuPAG... |
85,503 | MEASUREMENT OF GLOMERULAR FILTRATION RATE (GFR) (by Iohexol plasma level determination) | 1 | dx.doi.org/10.17504/protocols.io.14egn37wyl5d/v1 | https://www.protocols.click/view/measurement-of-glomerular-filtration-rate-gfr-by-i-cxq7xmzn | Daniela Cugini, Fabiola Carrara | TITLE: MEASUREMENT OF GLOMERULAR FILTRATION RATE (GFR) (by Iohexol plasma level determination)
AUTHORS: Daniela Cugini, Fabiola Carrara
[DESCRIPTION]
Glomerular Filtration Rate (GFR) value is widely accepted as the best overall index of kidney function in health and disease. The aim of this standad operative procedure... | ["[OBJECTIVE] To describe the procedure for plasma collection to ensure sample uniformity, quality and integrity.", "[SCOPE] This SOP applies to all visits where the protocol requires a collection of biological plasma samples.", "[INTRODUCTION] Glomerular filtration rate (GFR) is the standard measure of renal function... |
103,430 | Cryoprotection of mouse brain tissue | 0 | dx.doi.org/10.17504/protocols.io.261ge5x9jg47/v1 | https://www.protocols.io/view/cryoprotection-of-mouse-brain-tissue-dg9e3z3e | Lauren C. Faget, Thomas Hnasko | TITLE: Cryoprotection of mouse brain tissue
AUTHORS: Lauren C. Faget, Thomas Hnasko
[DESCRIPTION]
Fixed brains are submerged in 30% sucrose to cryoprotect prior to freezing and cutting cryosections.
[BEFORE_START]
Following perfusion and post-fixation in 4% ice-cold PFA (for 2 hrs, overnight, or as appropriate for in... | ["[Procedure] Make 30% sucrose solution by dissolving 30 g of sucrose (BP220-1, Fisher) in 100 mL of 1X PBS (BP3991, Fisher) pH 7.4", "[Procedure] Fill tube (20 mL glass scintillation vials or 15 mL conical centrifuge tubes are classically used) with ~ 10 mL of sucrose and transfer fixed brain tissue into tube, gently ... |
20,957 | UC Davis - Intraperitoneal Insulin Tolerance Test | null | dx.doi.org/10.17504/protocols.io.yp5fvq6 | null | Fawaz G. Haj | TITLE: UC Davis - Intraperitoneal Insulin Tolerance Test
AUTHORS: Fawaz G. Haj
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">
An intraperitoneal insulin tolerance test or ipITT is designed to determine the sensitivity... | ["Fast mice for 4 h only by taking away food early in the morning (7:00am).", "Calibrate the glucose meter according to the manufacturer’s instructions.", "Deprive mice from water then remove approximately 5μl of blood (one drop) from the tail via a tail tip cut and transfer directly onto a glucose indicator strip.", "... |
20,480 | Neural progenitor expansion | null | dx.doi.org/10.17504/protocols.io.x88frzw | null | Celeste Karch, Rita Martinez, Jacob Marsh | TITLE: Neural progenitor expansion
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh
[STEPS]
?. Using the pre-coated PLO-laminin plate, aspirate laminin from 2-3 wells. Remove supernatant from 15 mL conical tube containing 2 wells of neural rosette clusters. See protocol below.
?. Add appropriate amount of neural ind... | ["Using the pre-coated PLO-laminin plate, aspirate laminin from 2-3 wells. Remove supernatant from 15 mL conical tube containing 2 wells of neural rosette clusters. See protocol below.", "Add appropriate amount of neural induction media (NIM) to achieve a final volume of per well. Pipet clusters up and down breaking t... |
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