id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.rcpd2vn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The complexity of ADCs consisting of payloads, and conjugated antibodies and<a href="https://www.creative-biolabs.com/adc/classify-adc-linkers-7.htm" target="_blank" rel="noopener noreferrer"> ADC linkers </a>creates difficulties in developing new pharmacokinetic research met... | [] |
39,956 | PCR amplification of 16S rRNA, 18S rRNA, and nifH genes in coral samples | 4 | dx.doi.org/10.17504/protocols.io.bi9ukh6w | https://www.protocols.io/view/pcr-amplification-of-16s-rrna-18s-rrna-and-nifh-ge-bi9ukh6w | Molly Moynihan | TITLE: PCR amplification of 16S rRNA, 18S rRNA, and nifH genes in coral samples
AUTHORS: Molly Moynihan
[STEPS]
?. [Primers]
ABCD1GenePrimerSequenceReference216S rRNAB969F 5'-ACGCGHNRAACCTTACC-3'Comeau 2011316S rRNABA1406R5'-ACGGGCRGTGWGTRCAA-3'Comeau 20114518S rRNAUNonMetF5'-GTGCCAGCAGCCGCG-3'Bower 2004618S rRNAUNonM... | ["[Primers]\nABCD1GenePrimerSequenceReference216S rRNAB969F 5'-ACGCGHNRAACCTTACC-3'Comeau 2011316S rRNABA1406R5'-ACGGGCRGTGWGTRCAA-3'Comeau 20114518S rRNAUNonMetF5'-GTGCCAGCAGCCGCG-3'Bower 2004618S rRNAUNonMetR5'-TTTAAGTTTCAGCCTTGCG-3'Bower 20047818S rRNAV4 18S For5'-CCAGCASCYGCGGTAATTCC-3'Piredda 2017918S rRNAV4 18S R... |
84,255 | Explant Surgery: Chronic recoverable Neuropixels in mice | 1 | dx.doi.org/10.17504/protocols.io.bp2l6113dvqe/v4 | https://www.protocols.click/view/explant-surgery-chronic-recoverable-neuropixels-in-cwh7xb9n | Emily A Aery Jones | TITLE: Explant Surgery: Chronic recoverable Neuropixels in mice
AUTHORS: Emily A Aery Jones
[DESCRIPTION]
This protocol collection explains how to build a low-cost, lightweight system to implant 1 Neuropixels 1.0 probe or 2 Neuropixels 2.0 probes into mice, record during freely moving behavior, then recover the probes... | ["[Prepare mouse] Set O2 flow rate to 1-1.5L/min and isoflurane to 3%. Place mouse in anesthetic chamber.", "[Prepare mouse] When breathing has slowed to 1Hz, move animal to the toothbar. Switch isoflurane from chamber to nosecone. Wait until unresponsive to pedal reflex test, then lower to 1.5% isoflurane.", "[Prepare... |
86,471 | GI Transit Assay | 1 | dx.doi.org/10.17504/protocols.io.eq2lyjj3mlx9/v1 | https://www.protocols.io/view/gi-transit-assay-cypfxvjn | Sabina Marciano, Roberta Marongiu | TITLE: GI Transit Assay
AUTHORS: Sabina Marciano, Roberta Marongiu
[DESCRIPTION]
This test is used to check for possible signs of constipation and alteration in the gut transit
[STEPS]
1. Fast mice for 6 hours
2. Administer 0.3 mL Carmine red per mouse via oral gavage
3. Monitor the mice every 15 minutes for up to 5... | ["Fast mice for 6 hours", "Administer 0.3 mL Carmine red per mouse via oral gavage", "Monitor the mice every 15 minutes for up to 5 hours for the appearance of the first red fecal pellet", "The time from gavage to passage of first red pellet is recorded as total intestinal transit time."] |
69,419 | Time-course live imaging of maize and sorghum protoplasts | 4 | dx.doi.org/10.17504/protocols.io.4r3l27me3g1y/v1 | https://www.protocols.io/view/time-course-live-imaging-of-maize-and-sorghum-prot-cf2jtqcn | Rachel Baschieri, Thai Dao, Samuel Leiboff | TITLE: Time-course live imaging of maize and sorghum protoplasts
AUTHORS: Rachel Baschieri, Thai Dao, Samuel Leiboff
[DESCRIPTION]
A protoplast is a living plant, fungal, or bacterial cell with the cell wall removed. Protoplasts offer a simplified system for studies of gene expression compared to more complex whole-pl... | ["[Day 1 - Protoplast Isolation and Transfection] Fill a glass petri dish with the filter sterilized enzyme solution, and set aside.", "[Day 1 - Protoplast Isolation and Transfection] Harvest 10-14 day old seedlings and submerge in 5% bleach 1% Tween solution for 1min., remove and rinse seedlings thoroughly in autoclav... |
69,196 | HMW DNA extraction protocol for ferns | 4 | dx.doi.org/10.17504/protocols.io.bp2l69kb1lqe/v1 | https://www.protocols.io/view/hmw-dna-extraction-protocol-for-ferns-cftktnkw | Geferson Fernando Metz, Rafael Plá Matielo Lemos, Cristiane Barbosa D'Oliveira Matielo, Tiego Ferreira, Filipe Victoria | TITLE: HMW DNA extraction protocol for ferns
AUTHORS: Geferson Fernando Metz, Rafael Plá Matielo Lemos, Cristiane Barbosa D'Oliveira Matielo, Tiego Ferreira, Filipe Victoria
[DESCRIPTION]
Among the difficulties encountered in a laboratory, simple situations such as efficiently disinfecting fern spores and extracting l... | ["[Plant material sterilization] Recover and store the spores together with sporangia in 1.5 mL microtubes until one-third of the tube was filled and then stored at -20 °C until disinfection.", "[Extraction of high-molecular-weight DNA]", "[Extraction of high-molecular-weight DNA] Homogenize by inversion 10 times and i... |
null | null | null | dx.doi.org/10.17504/protocols.io.ptsdnne | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes how to transform chemically competent <em>Vibrio natriegens</em> cells.</p>
<p>The protocol was described and published by Weinstock et al., 2016</p>
[BEFORE_START]
<p>You need:</p>
<ul>
<li>Chemically competent <em>V. natriegens </em>cells</li>
<li>B... | [] |
31,406 | Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) | null | dx.doi.org/10.17504/protocols.io.bawnifde | null | Addgene the nonprofit plasmid repository | TITLE: Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning)
AUTHORS: Addgene the nonprofit plasmid repository
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes plasmid cloning by restriction enzyme digest (aka subcloning). To see the full abstract and additional ... | ["[Design (Choosing enzymes)]\nMany DNA analysis tools, including Addgene’s Sequence Analyzer, allow you to identify which restriction sites are present in a given sequence. When selecting restriction enzymes, you want to choose enzymes that:Flank your insert, but do not cut within your insertAre in the desired locatio... |
null | null | null | dx.doi.org/10.17504/protocols.io.c6zzf5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<strong>Abstract</strong><br />Ocean viruses are abundant, ubiquitous, and play important roles in global biogeochemical cycles through mortality, horizontal gene transfer and manipulation of host metabolism. However, the ability to link viruses to their hosts in a high-throughp... | [] |
44,916 | OG1RF_transposon_mutant_library_protocol | 4 | null | https://www.protocols.io/view/og1rf-transposon-mutant-library-protocol-bp4umqww | Elizabeth Fozo | TITLE: OG1RF_transposon_mutant_library_protocol
AUTHORS: Elizabeth Fozo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Generation of Transposon Mutant Library</span></div></div>
[STEPS]
?. [Transform transposase-carrying plasmid (pCJK55) into E. faecalis strain of... | ["[Transform transposase-carrying plasmid (pCJK55) into E. faecalis strain of interest.]\nInoculate 5-10 mL BHI with strain of interest (can use fusidic acid at 25 µg/mL) and incubate overnight at 37°C.", "[Transform transposase-carrying plasmid (pCJK55) into E. faecalis strain of interest.]\nDilute o/n culture 1:10 in... |
41,237 | VEPextended | 5 | dx.doi.org/10.17504/protocols.io.bkhvkt66 | https://www.protocols.io/view/vepextended-bkhvkt66 | Israel Aguilar Ordoñez | TITLE: VEPextended
AUTHORS: Israel Aguilar Ordoñez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">'VEPextended' is a tool, implemented in Nextflow, that annotates called variants using Variant Effect Predictor (VEP) and additional plugins that implement functionalities, that are not included in var... | ["[Pre-processing]\nFilter VCFRemove the variants that did not have any copy of the alternative allele.Dependencies:\na) Filter and remove when there was not an alternative allele in the VCF file.vcf.gz, to only conserve found variants.b) Compress the filtered file using one thread for compression.c) Make and index out... |
21,646 | Vandy – Post Clamp Anesthesia | null | dx.doi.org/10.17504/protocols.io.zdnf25e | null | Louise Lantier | TITLE: Vandy – Post Clamp Anesthesia
AUTHORS: Louise Lantier
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">After the last sample is taken for the clamp, the mouse is anesthetized in order to harvest tissues. It is ve... | ["Draw up the desired volume of diluted Pentobarb (5mg/ml in saline) in a blunted syringe. • To give 35mg/kg, multiply body weight (grams ) by 7, to get the volume to inject in uL. • Pentobarb (uL) = BW (g) * 7", "Steadily inject the pentobarbital in the jugular line of the catheterized mouse.", "If the mouse i... |
98,299 | CODA (part 6): register the nuclear coordinates and construct 3D cell matrix | HuBMAP | JHU-TMC | 0 | dx.doi.org/10.17504/protocols.io.n2bvjnrnxgk5/v2 | https://www.protocols.io/view/coda-part-6-register-the-nuclear-coordinates-and-c-db832ryn | Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz | TITLE: CODA (part 6): register the nuclear coordinates and construct 3D cell matrix | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz
[DESCRIPTION]
Using the registration transformations calculated in low-resolution (as described CODA (part 2): calculate re... | ["[register the nuclear coordinates] As input, this function needs the path to the low-resolution images that were registered in CODA-part2 (pth1x), the path containing the coordinates calculated in CODA-part4 (pthcoords), and the scale factor between these images. For cell detection performed in 10x and registration c... |
null | null | null | dx.doi.org/10.17504/protocols.io.irgcd3w | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
50,421 | Optogenetic experiments with iLID system | 1 | dx.doi.org/10.17504/protocols.io.bvgvn3w6 | https://www.protocols.io/view/optogenetic-experiments-with-ilid-system-bvgvn3w6 | Andrés Guillén-Samander, Pietro De Camilli | TITLE: Optogenetic experiments with iLID system
AUTHORS: Andrés Guillén-Samander, Pietro De Camilli
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol details experiments with the iLID optogenetic system as performed to acutely recruit Miro to mitochondria in </span><a href="https:/... | ["[Optogenetic experiments with iLID system]\nPlate the COS-7 cells for imaging and transfect each MatTek dish (see Cell Culture, Transfection and Imaging Protocol) with the plasmids containing the bait (construct encoding iLID) and the prey (construct encoding SspB peptide).\nAs an example, in the case of Guillén-Sama... |
41,069 | BG11 hypersaline medium | 1 | dx.doi.org/10.17504/protocols.io.bkcmksu6 | https://www.protocols.io/view/bg11-hypersaline-medium-bkcmksu6 | Tanja Bosak | TITLE: BG11 hypersaline medium
AUTHORS: Tanja Bosak
[STEPS]
?. Add the following to a beaker and dissolve using a stir bar on a magnetic stir plate. ABC1BG11 hypersaline mediumamountunit2initial Milli-Q water968ml3NaCl49.8g 4NaNO31.5g5Na2CO30.02g6KCl1.3867g7MgCl2*6H2O8.569g8MgSO4*7H2O6.4998g9CaCl2*2H2O2.042g10Stock A... | ["Add the following to a beaker and dissolve using a stir bar on a magnetic stir plate. ABC1BG11 hypersaline mediumamountunit2initial Milli-Q water968ml3NaCl49.8g 4NaNO31.5g5Na2CO30.02g6KCl1.3867g7MgCl2*6H2O8.569g8MgSO4*7H2O6.4998g9CaCl2*2H2O2.042g10Stock A10ml11Stock B10ml12Stock C10ml13Stock 5 (trace metals)1ml14Vit... |
95,766 | IMMUNOFLUORESENCE ANTIBODY (IF) STAINING PROTOCOL | 1 | dx.doi.org/10.17504/protocols.io.36wgq3x33lk5/v1 | https://www.protocols.io/view/immunofluoresence-antibody-if-staining-protocol-c9rwz57e | Scott Vermilyea | TITLE: IMMUNOFLUORESENCE ANTIBODY (IF) STAINING PROTOCOL
AUTHORS: Scott Vermilyea
[DESCRIPTION]
This is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.
[GUIDELINES]
Principle:
For antibody staining to be successful, most FFPE tissue requires antigen retrieval of some kind. ... | ["[DAY 1: Deparaffinize tissue] Either place slides on a slide warmer with temperature set to approx. 57 °C. Leave the slides on the warming plate until the paraffin looks melted on all of the slides (about 0-15 min).", "[DAY 1: Deparaffinize tissue] Put slides in a 60 °C oven for about 30 min.", "[DAY 1: Preferred An... |
null | null | null | dx.doi.org/10.17504/protocols.io.hgib3ue | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
16,610 | Real-Time Q-PCR Protocol for chicken sex identification. | null | dx.doi.org/10.17504/protocols.io.ugaetse | null | Liyan He, Priscila Martins, Joris Huguenin, Thi-Nhu-Ngoc Van, Taciana Manso, Therese Galindo, Lise Catherinot, Franck Molina, Julien Espeut | TITLE: Real-Time Q-PCR Protocol for chicken sex identification.
AUTHORS: Liyan He, Priscila Martins, Joris Huguenin, Thi-Nhu-Ngoc Van, Taciana Manso, Therese Galindo, Lise Catherinot, Franck Molina, Julien Espeut
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = ":justify;">This is a Rea... | ["[Lysis of chicken embryo's tissue and DNA extraction]\n1. Prepare the Lysis Buffer: ABC1Composition for 100 ml BufferQuantity (Volume)Final Concentration2Chelex Resin (Biorad Chelex 100)10g10%3SDS 20 %1 ml0.2%4Tris 1M pH81 ml10 mM5Nuclease Free Waterqsp 100 ml2. Put 150 µl of lysis buffer in each 1.5 ml tube or well... |
null | null | null | dx.doi.org/10.17504/protocols.io.mn9c5h6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.cpyvpv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the quick protocol for the Q5® Site-Directed Mutagenesis Kit (E0554)
[BEFORE_START]
Primers should be designed with 5´ ends annealing back-to-back. We recommend using the NEB online design software, <a href="http://nebasechanger.neb.com/" target="_blank">... | [] |
98,232 | Histology: Analysis Protocol | 0 | dx.doi.org/10.17504/protocols.io.kxygxye1kl8j/v1 | https://www.protocols.io/view/histology-analysis-protocol-db6y2rfw | Sasha Burwell | TITLE: Histology: Analysis Protocol
AUTHORS: Sasha Burwell
[DESCRIPTION]
This protocol details the analysis of histology images, including usage of Matlab codes to draw regions of interest (ROIs) and ilastik software to perform pixel classification.
[STEPS]
SECTION: Analysis Protocol
1. Go to the folder with all of ... | ["[Analysis Protocol] Go to the folder with all of the .vsi images from one brain.", "[Analysis Protocol] Use the \n \n Matlab code to manually draw regions of interest (ROIs) of the left VTA and right VTA per section.", "[Analysis Protocol] Change the channels and intensities (top right table) as needed to visualize y... |
null | null | null | dx.doi.org/10.17504/protocols.io.ui7euhn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
SECTION: Concentration of the O marina cultures
?.
SECTION: Linearization of the DNA that will be transformed into O marina
?.
SECTION: Preparation of the reagents for the particle bombardment
?.
SECTION: Preparation of the gold particles
?.
SECTION: Preparation of the cultures on... | ["[Concentration of the O marina cultures] O marina are fragile and a high number of organisms die if subjected to high speed centrifugation. In contrast, since they are strong swimmers, if the speed used to pull them down is too slow, it is difficult to force them to pellet. We adapted two methods to increase the numb... |
5,648 | Enzymatic Spectrophotometry Assay to Measure D-, L-, and Total Lactate in Canine Feces | null | dx.doi.org/10.17504/protocols.io.hrqb55w | null | Amanda Blake, Jonathan Lidbury, Joerg Steiner, Jan Suchodolski | TITLE: Enzymatic Spectrophotometry Assay to Measure D-, L-, and Total Lactate in Canine Feces
AUTHORS: Amanda Blake, Jonathan Lidbury, Joerg Steiner, Jan Suchodolski
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Aliquot feces]
Aliquot 120-130 mg feces into 1.5 mL microcentrifuge tube. Store at -80°C unt... | ["[Aliquot feces]\nAliquot 120-130 mg feces into 1.5 mL microcentrifuge tube. Store at -80°C until deproteinization.", "[Begin deproteinization. Add TEA buffer]\nMake 0.1 M triethanolamine buffer (pH 9.15) using scale for accuracy (to the 0.01 mg). Then add 750 μL buffer to each aliquot. Vortex and place in refrigerato... |
58,215 | Peroxidase/DAB protocol | 1 | dx.doi.org/10.17504/protocols.io.b44fqytn | https://www.protocols.io/view/peroxidase-dab-protocol-b44fqytn | Christiana.bjorkli | TITLE: Peroxidase/DAB protocol
AUTHORS: Christiana.bjorkli
[DESCRIPTION]
Peroxidase/DAB protocol for chromogen labelling
[STEPS]
1. Leave sections in 0.125 Molarity (M) phosphate buffer (PB) at 60 °C
2. Rinse sections 2 x 10min in PB
3. Rinse sections 1 x 10 min in 0,5% TBS-Tx
3.1. Tris: 3,03 gram/500 mlH2O
NaCl... | ["Leave sections in 0.125 Molarity (M) phosphate buffer (PB) at 60 °C", "Rinse sections 2 x 10min in PB", "Rinse sections 1 x 10 min in 0,5% TBS-Tx", "Tris: 3,03 gram/500 mlH2O\nNaCl: 4,48 gram/500 mlH2O\nTriton-X-100: 2,5 ml/500 mlH2O\n\nUse HCl to adjust the pH 8. Store in refrigerator for up to one week.", "Dilute 0... |
69,779 | Criteria for performing total knee arthroplasty without postoperative intensive care monitoring | 1 | dx.doi.org/10.17504/protocols.io.bp2l6b325gqe/v2 | https://www.protocols.io/view/criteria-for-performing-total-knee-arthroplasty-wi-cgdtts6n | Fabricio Loures, João Henrique Reis, Marcelo de Oliveira Campos, Guilherme Morgado Runco, Luísa Doim Vieira Fonseca, Luciana Ferreira Xavier, Nelson Hiroyuki Miyabe Ooka, Liszt Palmeira de Oliveira | TITLE: Criteria for performing total knee arthroplasty without postoperative intensive care monitoring
AUTHORS: Fabricio Loures, João Henrique Reis, Marcelo de Oliveira Campos, Guilherme Morgado Runco, Luísa Doim Vieira Fonseca, Luciana Ferreira Xavier, Nelson Hiroyuki Miyabe Ooka, Liszt Palmeira de Oliveira
[DESCRIPT... | [] |
61,615 | In Vitro GCase Activity Assay (Total Cell Lysate) | 4 | dx.doi.org/10.17504/protocols.io.n2bvj625nlk5/v1 | https://www.protocols.io/view/in-vitro-gcase-activity-assay-total-cell-lysate-b8eprtdn | Laura Smith | TITLE: In Vitro GCase Activity Assay (Total Cell Lysate)
AUTHORS: Laura Smith
[DESCRIPTION]
Taurocholate inhibits the cytosolic B–glucosidase (GBA2) and activates lysosomal B–glucosidase (GBA1, a.k.a glucocerebrosidase (GCase)).In cultured cells the pH optimum is increased in the presence of taurocholate.Cultured cel... | ["[Principle] Taurocholate inhibits the cytosolic –glucosidase (GBA2) and activates lysosomal –glucosidase (GBA1, a.k.a glucocerebrosidase (GCase)).In cultured cells the pH optimum is increased in the presence of taurocholate.Cultured cells are assayed without taurocholate at pH 4.5 and with taurocholate at pH 5.4.In... |
75,293 | CTAB Extraction Protocol for Sediment | 4 | dx.doi.org/10.17504/protocols.io.ewov1ozkolr2/v1 | https://www.protocols.io/view/ctab-extraction-protocol-for-sediment-cmr5u586 | Cameron R. Turner | TITLE: CTAB Extraction Protocol for Sediment
AUTHORS: Cameron R. Turner
[DESCRIPTION]
Successfully used by Turner et al., 2015 to detect bigheaded Asian carp surface sedimentary DNA from experimental ponds and natural rivers
https://www.sciencedirect.com/science/article/pii/S000632071400442X
[STEPS]
SECTION: Sample ... | ["[Sample preparation] THAW the CTAB-preserved sediment sample in the fridge for no more than 1440 min\n\nDECONTAMINATE the exterior of the sample tube with 10 % bleach solution and rinse with reverse osmosis water", "[Sample preparation] VORTEX at max speed for 30 s\n\nINCUBATE at 60 °C for 10 min", "[Chloroform extra... |
null | null | null | dx.doi.org/10.17504/protocols.io.ds56g5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<span class="cit-title">This is a collection of protocols from:<br /></span><span class="cit-auth cit-auth-type-author">Linlin Yin</span><span class="cit-sep cit-sep-separator">, et al.</span><span class="cit-auth cit-auth-type-author"> (2015) <a href="http://www.genetics.org/co... | [] |
73,981 | Creed / Drago Check List | 1 | null | https://www.protocols.io/view/creed-drago-check-list-ckg5uty6 | Kausalia Vijayaragavan | TITLE: Creed / Drago Check List
AUTHORS: Kausalia Vijayaragavan
[DESCRIPTION]
Important to check after any Ionpath servicing.
The in house preset SIO and Sample Bias values are different from IonPath's default values.
The values were determined empirically (Hadeesha Piyadasa et.al.) to optimise organic (Noodle) nois... | ["[Check on SIO Voltage Settings] Go to HV Control Tab", "[Check on SIO Voltage Settings] Check on Voltage of Secondary Ion Optics (SIO) \n \n \nMake sure Lens1 voltage is at or to close 500V. Do not worry about the negative value. If it is at 1000V please contact Mike Angelo. 1000V is the default voltage setting for I... |
null | null | null | dx.doi.org/10.17504/protocols.io.sq2edye | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes a method to perform simultaneous extraction of RNA (using the Qiagen's miRNeasy Mini Kit), genomic DNA and protein from canine mast cell tumour or canine skin biopsies (in the form of 3mm cubes) preserved in RNA-Later.</p>
[BEFORE_START]
<p>You will ... | [] |
27,839 | seqFISH Tissue Preservation | null | dx.doi.org/10.17504/protocols.io.7e7hjhn | null | Long Cai, Nina Dar, Yiing Lin, Shin Lin | TITLE: seqFISH Tissue Preservation
AUTHORS: Long Cai, Nina Dar, Yiing Lin, Shin Lin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Tissue Preparation for seqFISH</div></div>
[STEPS]
?. [Fixation]
Clean surface with RNAzap. Make the following solutions.1L PBS 1x, RNAse-free1. take 1 L RNAse-free w... | ["[Fixation]\nClean surface with RNAzap. Make the following solutions.1L PBS 1x, RNAse-free1.\ttake 1 L RNAse-free water bottle2.\tuse serologic pipet and draw up 100 mL water into another container3.\tadd 100 mL of 10x RNase-free PBS4.\tmix thoroughly50% sucrose1.\ttare 1 L RNase-free disposable container2.\ttake 500... |
70,344 | Flow cytometry | 1 | dx.doi.org/10.17504/protocols.io.kqdg3956eg25/v1 | https://www.protocols.io/view/flow-cytometry-cgxgtxjw | gurvir.virdi | TITLE: Flow cytometry
AUTHORS: gurvir.virdi
[DESCRIPTION]
This protocol is for immunolabelling fixed midbrain dopaminergic neurons for flow cytometry analysis and downstream flow cytometry acquisition.
[STEPS]
SECTION: Sample preparation
1. Cells were washed once with PBS
SECTION: Sample preparation
2. The cells were... | ["[Sample preparation] Cells were washed once with PBS", "[Sample preparation] The cells were incubated with Accutase (Gibco) to generate a single-cell suspension for 5 min at 37 °C", "[Sample preparation] A cell suspension of 500k/ml was prepared in media", "[Sample preparation] Cells were then spun down at 200 x g, 5... |
58,220 | CTAB DNA Extraction of Plant Tissue | 1 | null | https://www.protocols.io/view/ctab-dna-extraction-of-plant-tissue-b44kqyuw | Lynn Doran | TITLE: CTAB DNA Extraction of Plant Tissue
AUTHORS: Lynn Doran
[DESCRIPTION]
CTAB DNA extraction using chloroform/isoamyl alcohol wash, precipitation in isopropanol, and ethanol rinses for leaf tissue.
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.h3vb8n6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes two methods for assessing whether soluble factors alone or a combination of soluble factors and T cells are responsible for bystander killing: (1) Supernatant transfer assays and (2) Transwell® assays.</p>
[STEPS]
?.
?.
?.
?. | [] |
23,970 | Making glycerol stocks of bacteria | null | dx.doi.org/10.17504/protocols.io.3nagmae | null | Cristian Riccio | TITLE: Making glycerol stocks of bacteria
AUTHORS: Cristian Riccio
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Freezing down a bacterial culture for long-term storage.</div></div>
[STEPS]
?. Make sure you have autoclaved glycerol, cryovials and a fresh bacterial culture in liquid b-broth or LB.... | ["Make sure you have autoclaved glycerol, cryovials and a fresh bacterial culture in liquid b-broth or LB. If you don't have a fresh bacterial culture, you can grow them in b-broth or LB medium at 180 rpm in flasks.", "Prepare a sterile 50% glycerol/50% DEPC water solution.", "Add 1 ml 50/50 solution to as many cryovia... |
25,952 | Protocol for derivatization and determination of structural monosaccharides in crude fungal exopolysaccharide | null | dx.doi.org/10.17504/protocols.io.5j8g4rw | null | tayebeh fooladi, Mohammad Reza Soudi, Majid M. Heravi, Sanaz Karimian | TITLE: Protocol for derivatization and determination of structural monosaccharides in crude fungal exopolysaccharide
AUTHORS: tayebeh fooladi, Mohammad Reza Soudi, Majid M. Heravi, Sanaz Karimian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"></di... | [] |
50,398 | Schedule of Events (Appendix 1 of "Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus") | 1 | dx.doi.org/10.17504/protocols.io.bvf6n3re | https://www.protocols.io/view/schedule-of-events-appendix-1-of-34-safety-and-eff-bvf6n3re | Stephen.Gitelman , Jeffrey A. Bluestone | TITLE: Schedule of Events (Appendix 1 of "Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus")
AUTHORS: Stephen.Gitelman , Jeffrey A. Bluestone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is Appendix 1 of "Safety and Efficacy of Imatini... | [] |
53,821 | Agarose gel pads for live Ashbya imaging | 4 | null | https://www.protocols.io/view/agarose-gel-pads-for-live-ashbya-imaging-bys5pwg6 | ameya.jalihal | TITLE: Agarose gel pads for live Ashbya imaging
AUTHORS: ameya.jalihal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A protocol to prepare agarose gel pads for live Ashbya imaging.</div></div>
[STEPS]
?. [Resuspension of Ashbya]
Make 20 mL 1X LFM stock before you startAlways check LFM tube for c... | ["[Resuspension of Ashbya]\nMake 20 mL 1X LFM stock before you startAlways check LFM tube for contaminationReconstitute with ddH2O", "[Making agarose pads]\nDepression slides live on Grace's desk. These are expensive, and we reuse them. Don't throw these out! Wash thoroughly with water and ethanol, dry them, and replac... |
79,040 | Paintbrush method for C. elegans handling for microinjections | 4 | dx.doi.org/10.17504/protocols.io.5qpvor1r9v4o/v1 | https://www.protocols.io/view/paintbrush-method-for-c-elegans-handling-for-micro-cre8v3hw | Theresa V Gibney, Ariel M Pani | TITLE: Paintbrush method for C. elegans handling for microinjections
AUTHORS: Theresa V Gibney, Ariel M Pani
[DESCRIPTION]
See PDF attachment for protocol and links to videos.
This protocol describes a method for using a paintbrush to move C. elegans throughout the microinjection process. This method accelerates the ... | [] |
51,282 | Higienização/esterilização de actígrafos - ActTrust | 5 | dx.doi.org/10.17504/protocols.io.bwbspane | https://www.protocols.io/view/higieniza-o-esteriliza-o-de-act-grafos-acttrust-bwbspane | Daniel Vartanian | TITLE: Higienização/esterilização de actígrafos - ActTrust
AUTHORS: Daniel Vartanian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Este protocolo faz parte do processo de coleta de dados actigráficos do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO). Ele foi desenhado para os actígrafos... | ["[Trello]\nAdicione o cartão da tarefa no quadro de trabalho do seu projeto (somente para projetos que usam Kanban)\nAbra o quadro de trabalho de seu projeto e adicione um cartão chamado Realizar a higienização/esterilização de actígrafos na lista Em andamento. No cartão, adicione uma etiqueta chamada Processamento e ... |
52,619 | Multiplex SARS-CoV-2 RT-qPCR protocol | 4 | null | https://www.protocols.io/view/multiplex-sars-cov-2-rt-qpcr-protocol-bxmjpk4n | Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker, F... | TITLE: Multiplex SARS-CoV-2 RT-qPCR protocol
AUTHORS: Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Ni... | ["Run the RT-qPCR program:Time (s)Temp (°C)1x300551x209540x3953058\nTime (s)Temp (°C)1x300551x209540x3953058", "Prepare the RT-qPCR master mix: ReagentsVolume (1 reaction)4x TaqMan Fast Virus 1-Step Master Mix3.75 µlprimer/probe mix0.75 µlNuclease-free water 3.0 µlRNA7.5 µlTotal15 µl\nReagentsVolume (1 reaction)4x Taq... |
44,767 | Protocol for Freezing in OCT | 1 | dx.doi.org/10.17504/protocols.io.bpx7mprn | https://www.protocols.io/view/protocol-for-freezing-in-oct-bpx7mprn | Shin Lin, Yiing Lin | TITLE: Protocol for Freezing in OCT
AUTHORS: Shin Lin, Yiing Lin
[STEPS]
?. Place tissue sample in culture dish with ice cold PBS.
?. Cut tissue piece with disposable razor blade.
?. In mold (choose mold depending on size of tissue), place ice cold OCT to cover the bottom.
?. Place tissue in mold atop OCT. Fill mold ... | ["Place tissue sample in culture dish with ice cold PBS.", "Cut tissue piece with disposable razor blade.", "In mold (choose mold depending on size of tissue), place ice cold OCT to cover the bottom.", "Place tissue in mold atop OCT. Fill mold with OCT until tissue is covered.", "Place mold atop metal slab bathed in l... |
61,602 | X-tremeGENE™ HP DNA Transfection Reagent Protocol for transfection of SH-SY5Y cells | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy54rlx1/v1 | https://www.protocols.io/view/x-tremegene-hp-dna-transfection-reagent-protocol-f-b8eartae | Laura Smith | TITLE: X-tremeGENE™ HP DNA Transfection Reagent Protocol for transfection of SH-SY5Y cells
AUTHORS: Laura Smith
[DESCRIPTION]
SH-SY5Y cells were transfected with a pcDNA3.1 vector plasmid. Stable transfection was performed using the XtremeGENE reagent for 72 hours, and 400 µg/ml G418 antibiotics used as the selection... | ["Protocol from: https://www.sigmaaldrich.com/GB/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtghp-general-protocol", "[Cell Preparation for Transfection] Plate SH-SY5Y cells in 10mm2dish approximately 24 hours before transfection, making sure cells are at optima... |
null | null | null | dx.doi.org/10.17504/protocols.io.rqmd5u6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol with Ficoll-Paque/Histopaque for isolation of PBMCs from heparinized blood</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.j4tcqwn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A protocol for lithium acetate transformation of yeast that can be used to generate highly complex plasmid libraries (O(1E8) if starting with ~1L of culture) and can get over 1 million transformants from a single transformation (as described here). Optimized using yeast stra... | [] |
94,798 | Elucidating the roles of SOD3 correlated genes and reactive oxygen species in rare human diseases using a bioinformatic-ontology approach | 5 | dx.doi.org/10.17504/protocols.io.rm7vzxp14gx1/v1 | https://www.protocols.io/view/elucidating-the-roles-of-sod3-correlated-genes-and-c8tnzwme | Mark Stanworth, Shu-Dong Zhang | TITLE: Elucidating the roles of SOD3 correlated genes and reactive oxygen species in rare human diseases using a bioinformatic-ontology approach
AUTHORS: Mark Stanworth, Shu-Dong Zhang
[DESCRIPTION]
This is a gene discovery protocol utilising a single seed gene to create correlation lists. These lists are used to iden... | ["[Software and packages] Download and install the required software", "[Expression preparation] Download the RAW file for GSE2109\n \n \n\n\nExtract the CEL files using 7-zip", "[Expression preparation] Add 'affy' to the R library and normalise the data", "[SOD3 correlation data] Import the GSE2109_normalised.txt fil... |
63,855 | Simpli ACV Keto Shark Tank -Lose Stomach Fat This Product Really Work its real or fake ? | 3 | dx.doi.org/10.17504/protocols.io.q26g746e9gwz/v1 | https://www.protocols.io/view/simpli-acv-keto-shark-tank-lose-stomach-fat-this-p-cakpscvn | Namikajonesbuy | TITLE: Simpli ACV Keto Shark Tank -Lose Stomach Fat This Product Really Work its real or fake ?
AUTHORS: Namikajonesbuy
[DESCRIPTION]
Furthermore, they likewise give a 60-day unconditional promise in the event that the item doesn't proceed as guaranteed and you can reach out to the client support division and get e... | [] |
65,628 | Visualization of DNA of low concentration and molecular weight (DNA gel stain) | 4 | null | https://www.protocols.io/view/visualization-of-dna-of-low-concentration-and-mole-ccb4ssqw | Nadine Mowoh, Stephane Fadanka | TITLE: Visualization of DNA of low concentration and molecular weight (DNA gel stain)
AUTHORS: Nadine Mowoh, Stephane Fadanka
[DESCRIPTION]
After PCR amplification of DNA, an agarose gel electrophoresis is run to separate the DNA based on their size.
The agarose gel consists of microscopic pores that act as a molec... | ["[Visualization of DNA of low concentration and molecular weight]", "[Visualization of DNA of low concentration and molecular weight] To confirm visualization of DNA of low molecular weight\nDNA gel stain\n\nFollow the steps in preparing a 2% agarose gel as described in this protocol, \nWe use the TO-DMSO DNA gel stai... |
null | null | null | dx.doi.org/10.17504/protocols.io.rf2d3qe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is designed for 'around-the-horn' or 'divergent' PCR, where primers go around most or all of a plasmid but are pointed away from each other so they generate a linear product. Note that this protocol is written for Q5 polymerase, but works fine with other polymer... | [] |
25,584 | ATP synthase activity assay (radioactive) | null | dx.doi.org/10.17504/protocols.io.48qgzvw | null | Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann | TITLE: ATP synthase activity assay (radioactive)
AUTHORS: Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes how to detect synthesis of [α</spa... | ["[preparation]\nexpress and purify KaiCoptional: phosphorylate or dephosphorylate KaiC prior to analysis", "[incubation]\nprepare a 25 µl mastermix with 3 μM KaiC in ATP synthesis buffer (20 mM Tris/HCl (pH8), 150 mM NaCl, 0.5 mM EDTA, 5 mM MgCl2, 0.5 mM ATP)add 2 ul [α-32P]ADP (= 20 μCi) freeze one 10 µl aliquot at -... |
100,004 | 10x ATAC Genomics Sample Processing | 1 | dx.doi.org/10.17504/protocols.io.14egn7ndmv5d/v2 | https://www.protocols.io/view/10x-atac-genomics-sample-processing-ddwc27aw | Allen Institute for Brain Science | TITLE: 10x ATAC Genomics Sample Processing
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
Protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries from a pool of transposed nuclei.
[STEPS] | [] |
53,697 | PSI Open Fluor CAM script for measuring qE component of NPQ in Chlorella vulgaris | 1 | dx.doi.org/10.17504/protocols.io.byn9pvh6 | https://www.protocols.io/view/psi-open-fluor-cam-script-for-measuring-qe-compone-byn9pvh6 | Andrei Herdean | TITLE: PSI Open Fluor CAM script for measuring qE component of NPQ in Chlorella vulgaris
AUTHORS: Andrei Herdean
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a simple protocol that consists of </div><div class = "text-block">1) 10 minutes preillumination with far red light</div><div class... | ["[Script for FluorCAM software]\n; Quenching protocol with Actinic2\n ;with FilterWheel\n ;version November 11, 2020\n ;high-resolution CCD TOMI-2\n ;optimized number of measured frames\n ;Protocol duration 183s\n ;\n ADD1=0\n ADD2=10\n Act1=0\n TS=50ms\n include default.inc ;Includes standard options, do not remove ... |
103,471 | Nuclei Isolation from Human Meniscus for 10x Multiome | 0 | dx.doi.org/10.17504/protocols.io.eq2lyweqwvx9/v1 | https://www.protocols.io/view/nuclei-isolation-from-human-meniscus-for-10x-multi-dhap32dn | Takuya Sakamoto, Erpei Wang, Merissa Olmer, Martin Lotz | TITLE: Nuclei Isolation from Human Meniscus for 10x Multiome
AUTHORS: Takuya Sakamoto, Erpei Wang, Merissa Olmer, Martin Lotz
[DESCRIPTION]
This protocol describes isolation of nuclei from fresh-frozen human knee meniscus for use in Omics analyses, including RNA-sequencing of ATAC-sequencing. Tissue dissociation was t... | [] |
55,080 | Brain Homogenization and MSD Protocol for Mouse Brain and Serum | 4 | dx.doi.org/10.17504/protocols.io.kxygxprqkl8j/v1 | https://www.protocols.io/view/brain-homogenization-and-msd-protocol-for-mouse-br-bz2gp8bw | Scott Vermilyea | TITLE: Brain Homogenization and MSD Protocol for Mouse Brain and Serum
AUTHORS: Scott Vermilyea
[DESCRIPTION]
This protocol details about the homogenization and MSD protocol of the mouse brain using various panel kits.
[STEPS]
SECTION: Brain Homogenization Using Miltenyi gentleMACS Octodissociator
1. Weight out brain... | ["[Brain Homogenization Using Miltenyi gentleMACS Octodissociator] Weight out brain using pre-chilled C-tube.", "[Brain Homogenization Using Miltenyi gentleMACS Octodissociator] Use 2 mL RIPA (1x Halt PIC) per whole brain, 1 mL RIPA (1x Halt PIC) per half brain.", "[Brain Homogenization Using Miltenyi gentleMACS Octodi... |
null | null | null | dx.doi.org/10.17504/protocols.io.gybbxsn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="color: #000339; font-family: Raleway, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; w... | [] |
32,310 | Making and applying foliar fertiliser and pesticide solutions | null | dx.doi.org/10.17504/protocols.io.bbswinfe | null | Matema LE. Imakumbili | TITLE: Making and applying foliar fertiliser and pesticide solutions
AUTHORS: Matema LE. Imakumbili
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">This protocol describes how foliar and pesticide solutions can be made and applied to growing crops. </... | ["Making foliar fertiliser and pesticide solutions from manufactured productsGetting the application of foliar fertilisers and pesticides right starts with making correct chemical solution mixtures of foliar fertilisers and pesticides. A series of steps are however needed to achieve this. The steps shall be described b... |
null | null | null | dx.doi.org/10.17504/protocols.io.chdt25 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Principle is that CTAB is charging the anionic nucleotides whereby neutral polysaccharides/ melanins are remaining in supernatant. This method also uses urea with the idea that the presence of urea helps to solubilize hydrophobic compounds that would otherwise potentially intera... | [] |
36,060 | Assessment of oxidative phosphorylation and glycolysis in NK cells (Seahorse assays) | 1 | dx.doi.org/10.17504/protocols.io.yxmvmxqq5l3p/v1 | https://www.protocols.click/view/assessment-of-oxidative-phosphorylation-and-glycol-bff4jjqw | Philippa R Kennedy | TITLE: Assessment of oxidative phosphorylation and glycolysis in NK cells (Seahorse assays)
AUTHORS: Philippa R Kennedy
[DESCRIPTION]
The rate of glycolysis (as measured by acidification of the extracellular space) and the rate of oxidative phosphorylation (as calculated from the consumption of oxygen) are measured in... | ["[Day 1] NK cells are resuspended in Seahorse XF Assay Medium and added to a 96 well or 24 well flat bottom plate that has been coated with 0.01% poly-L-Lysine solution (Millipore Sigma) according to the manufacturer's instructions.", "[Day 1] The extracellular acidification rate (ECAR) and the oxygen consumption rate... |
89,556 | Heart rate | 1 | dx.doi.org/10.17504/protocols.io.kxygx355og8j/v1 | https://www.protocols.io/view/heart-rate-c3puymnw | Núria Peñuelas | TITLE: Heart rate
AUTHORS: Núria Peñuelas
[DESCRIPTION]
Heart rate measurement in mice.
[STEPS]
1. Place the animal in mild anesthesia with isofluorane at 1.5% and 1l/min O2.
2. Prepare the PhysioSuite apparatus(Kent Scientific). Put the clip sensor in the right hind paw. Let it stabilize for 30 seconds before starti... | ["Place the animal in mild anesthesia with isofluorane at 1.5% and 1l/min O2.", "Prepare the PhysioSuite apparatus(Kent Scientific). Put the clip sensor in the right hind paw. Let it stabilize for 30 seconds before starting the recording.", "When a stable heart rate is observed, perform 10 measurements.", "Calculate th... |
90,433 | Assessment of the in vitro trypanocidal activity | 4 | dx.doi.org/10.17504/protocols.io.81wgbxqd1lpk/v1 | https://www.protocols.io/view/assessment-of-the-in-vitro-trypanocidal-activity-c4i9yuh6 | Muhammad Muhsin Fathuddin | TITLE: Assessment of the in vitro trypanocidal activity
AUTHORS: Muhammad Muhsin Fathuddin
[DESCRIPTION]
The aim of this experiment is to assess the potential of a plant extract to fight off Trypanosoma species, including Trypanosoma brucei brucei, Trypanosoma evansi, etc., by testing it at concentrations ranging from... | ["[Antitrypansomal Activity] The infected rat to undergo euthanasia must have attained a blood parasitemia of log 8.4 or higher\n \n.", "[Antitrypansomal Activity] Euthanatized animal’s blood was dissolved in heparin (1 mL of heparin per 10 mL of blood) and mixed with glucose (0.1 g of glucose per 10 mL of blood).", "[... |
63,874 | Condor CBD Gummies Para Que Sirve – (2022) Top Best CBD Gummies to UseProducts for SaleCBD Gummy Brand Comparison ListIS IT SCAM OR LEGIT? | 1 | dx.doi.org/10.17504/protocols.io.rm7vzy2z5lx1/v1 | https://www.protocols.io/view/condor-cbd-gummies-para-que-sirve-2022-top-best-cb-camasc2e | JohnMillerre | TITLE: Condor CBD Gummies Para Que Sirve – (2022) Top Best CBD Gummies to UseProducts for SaleCBD Gummy Brand Comparison ListIS IT SCAM OR LEGIT?
AUTHORS: JohnMillerre
[DESCRIPTION]
Condor Cbd Gummies· Get Better Sleep At Night · Feeling Significantly better By Day · Construct Good Memories · Feel Fewer Aches Includ... | ["➢ Product Name — Condor CBD Gummies Para Que Sirve\n\n➢ Composition — Natural Organic Compound\n\n➢ Side-Effects — NA\n\n➢ Availability — Online\n\n➢ Rating — ⭐⭐⭐⭐⭐\n\n➢ Official Website (Sale Is Live) — Condor CBD Gummies Para Que Sirve\n\n➢VISIT THE OFFICIAL WEBSITE TO BUY TODAY SPECIAL OFFER!!\n\n➢VISIT THE OFFIC... |
12,442 | T-RFLP Sephadex purification and sample preparation | 1 | dx.doi.org/10.17504/protocols.io.36wgqwyogk57/v1 | https://www.protocols.io/view/t-rflp-sephadex-purification-and-sample-preparatio-qd2ds8e | Eva Petrova, Roey Angel | TITLE: T-RFLP Sephadex purification and sample preparation
AUTHORS: Eva Petrova, Roey Angel
[DESCRIPTION]
Purification of PCR-product after restriction digest. Samples proceeded on the capillary sequencer.
[BEFORE_START]
Materials:
digested PCR-product (100-200ng in 20ul)
96 well filter plates
96 well collection pl... | ["[Sephadex purification] Load 200μl Sephadex solution onto the 96 well filter plates (shake the Sephadex solution well before each usage!) (use the EppendorfMultipette with a 10ml tip), the no. of wells you load depends on the no. of samples,\nload filter plate on 96 well collection plate, \nplace the lit on top of th... |
null | null | null | dx.doi.org/10.17504/protocols.io.kv6cw9e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Insulin and glucagon hormones were detected in paraffin sections (4 μm thickness) of formalin-fixed human pancreases. Insulin and glucagon were detected immunohistochemically using mouse anti-human insulin mAb (ascites; Sigma) and mouse anti-porcine glucagon mAb (ascites; Sig... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gr4bv8w | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
53,692 | RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500 | 4 | dx.doi.org/10.17504/protocols.io.byn4pvgw | https://www.protocols.io/view/rt-qpcr-detection-of-process-controls-murine-norov-byn4pvgw | Jacquelina.Woods , rachel.rodriguez | TITLE: RT-qPCR Detection of Process Controls (Murine noroviurs and crAssphage) from Wastewater using AB 7500
AUTHORS: Jacquelina.Woods , rachel.rodriguez
[DESCRIPTION]
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV... | ["[Data Analysis] Adjust analysis settings to appropriate thresholds. All thresholds should be set at 0.01. Baseline start cycle should be set at 3 and baseline end cycle should be set at 10.", "[Data Analysis] Sample is valid if all of the following are observed: \n\n1. Negative RT-qPCR control is negative (undetermin... |
62,610 | GenXZ Keto Gummies *Read 8 Facts*100% Clinically Certified Ingredients! | 3 | dx.doi.org/10.17504/protocols.io.6qpvr6rwzvmk/v1 | https://www.protocols.io/view/genxz-keto-gummies-read-8-facts-100-clinically-cer-b9dsr26e | GenXZ Keto Gummies | TITLE: GenXZ Keto Gummies *Read 8 Facts*100% Clinically Certified Ingredients!
AUTHORS: GenXZ Keto Gummies
[DESCRIPTION]
GenXZ Keto Gummies is an excellent choice for everybody for losing weight that helps in reducing the extra fat from the body tone!
BUY NOW:- https://www.elitegross.com/buygenxzketogummies
[STEPS] | [] |
54,342 | Sample Collection and Measurement of Serum Neurofilament Light (NfL) | 1 | dx.doi.org/10.17504/protocols.io.bzbep2je | https://www.protocols.io/view/sample-collection-and-measurement-of-serum-neurofi-bzbep2je | Huw Morris, Donald Grosset, Amanda Heslegrave, Henrik Zetterberg | TITLE: Sample Collection and Measurement of Serum Neurofilament Light (NfL)
AUTHORS: Huw Morris, Donald Grosset, Amanda Heslegrave, Henrik Zetterberg
[DESCRIPTION]
This protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantag... | ["[Blood Sample Collection and Serum Preparation] At enrolment, collect 10 mL of venous blood from each participant in Serum Separator Tubes (SST): https://www.fishersci.co.uk/shop/products/vacutainer-sst-ii-advance-tubes/12927696", "[Blood Sample Collection and Serum Preparation] Within 1 hour of collection, centrifug... |
null | null | null | dx.doi.org/10.17504/protocols.io.gi3bugn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The <strong>Zyppy™ Plasmid Miniprep Kit</strong> features a <strong>Pellet-Free™</strong> modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulate... | [] |
20,720 | PCR Reaction Optimization | null | dx.doi.org/10.17504/protocols.io.ygqftvw | null | Kenneth Schackart | TITLE: PCR Reaction Optimization
AUTHORS: Kenneth Schackart
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">How to run nucleic acid amplification using the Thermo scientific PCR Master Mix kit.</div><div class = "text-block">Each reaction produces 50 μL.</div><div class = "text-block"><span>For the ... | ["[Prepare Work Area]\nSpray entire work area with 70% EtOH including pipettes, tip holder used for holding PCR tubes, and work surface. Wipe with a paper towel.", "[Prepare Work Area]\nSpray entire work area with RNAway.", "[Gather Materials]\nTake styrofoam container to Marley 527 (directly across from Marley 509) an... |
null | null | null | dx.doi.org/10.17504/protocols.io.ngydbxw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is for performing CITE-seq only. </p>
<p> </p>
<p>Cellular Indexing of Transcriptomes and Epitopes by Sequencing (<a href="https://www.nature.com/articles/nmeth.4380" target="_blank">CITE-seq</a>) is a multimodal single cell phenotyping method developed in the <... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.emqbc5w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is a highly reproducible method for creating hit science videos. Guaranteed to lead to millions of views, fame, faculty position, tenure, and stable funding.<br /><br />* Legal disclaimer: This is not a peer reviewed protocol. Therefore, all claims should be taken with as m... | [] |
76,972 | Roadmap to the study of gene and protein phylogeny and evolution - a practical guide | 5 | dx.doi.org/10.17504/protocols.io.36wgq77e3vk5/v5 | https://www.protocols.io/view/roadmap-to-the-study-of-gene-and-protein-phylogeny-cpekvjcw | Florian G Jacques, Kristian Pietras, Paulina Bolivar, emma.hammarlund | TITLE: Roadmap to the study of gene and protein phylogeny and evolution - a practical guide
AUTHORS: Florian G Jacques, Kristian Pietras, Paulina Bolivar, emma.hammarlund
[DESCRIPTION]
Developments in sequencing technologies and the sequencing of an ever-increasing number of genomes have revolutionised studies into bi... | ["[Introduction] We provide a step-by-step protocol for non-bioinformatic users to reconstruct the phylogeny and evolutionary history of genes or proteins. \n\nWe present a compilation of DNA and protein databases, as well as bioinformatic tools for molecular phylogenetic reconstruction, and a wide range of studies on ... |
72,872 | High-Efficiency Yeast Electroporation | 4 | dx.doi.org/10.17504/protocols.io.5qpvorr69v4o/v3 | https://www.protocols.click/view/high-efficiency-yeast-electroporation-cjegujbw | Stephanie Hood, Jeffrey A Lewis | TITLE: High-Efficiency Yeast Electroporation
AUTHORS: Stephanie Hood, Jeffrey A Lewis
[DESCRIPTION]
For when extremely high transformation efficiencies are needed (e.g., for transforming plasmid DNA libraries). Adapted from An improved yeast transformation method for the generation of very large human antibody librari... | ["[2 days before starting experiment] Streak out yeast strains to be transformed onto YPD agar and grow at 30°C for 2 days.", "[Day before experiment] For each transformation, inoculate cells into 4 separate tubes containing 5 ml of YPD, and grow to saturation overnight at 30°C with 270 rpm orbital shaking.", "[Day of ... |
94,504 | Molecular Cloning- Gibson and LR reactions | 4 | null | https://www.protocols.io/view/molecular-cloning-gibson-and-lr-reactions-c8igzubw | Melissa Hoyer, Harper JW | TITLE: Molecular Cloning- Gibson and LR reactions
AUTHORS: Melissa Hoyer, Harper JW
[DESCRIPTION]
The endoplasmic reticulum (ER) has a vast proteomic landscape to preform many diverse functions including protein and lipid synthesis, calcium ion flux, and inter-organelle communication. The ER proteome is remodeled in p... | ["[Gateway technology cloning] LR reaction: Add pDONOR vectors and pDEST vectors to a Gateway‱ LR Clonase‱ II Enzyme mix supplemented with TE buffer. Gateway‱ LR Clonase‱ II Enzyme mix (cat 11791020) https://www.thermofisher.com/order/catalog/product/11791020\n\n• 1–7 μL entry clone (50–150 ng) • 1 μL destination vec... |
34,082 | Assembling LED Controller Electronics | null | dx.doi.org/10.17504/protocols.io.bdiai4ae | null | Jakub Nedbal | TITLE: Assembling LED Controller Electronics
AUTHORS: Jakub Nedbal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The LED controller electronics regulates the LED current, adjusting the irradiance of the sample according the experimental needs. It also powers the fans cooling down the LEDs.</div><d... | ["Assembly of SMD ComponentsStart the assembly with all the SMD components first.Use a thin solder wire, solder flux, and watchmaker's tweezers to help with the soldering. The SMD components that need to be soldered are highlighted in the image below:", "Adding the Power Supply ConnectionIn this step, the power supply ... |
null | null | null | dx.doi.org/10.17504/protocols.io.gmebu3e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>These protocols accompany the following <em>GigaScience</em> publication:</p>
<p> </p>
<p>Jihoon Jo, et al. (2016): Draft genome of the sea cucumber Apostichopus japonicus and genetic polymorphism among color variants. <em>GigaScience</em>...</p>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.k2scyee | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The following protocol is optimized to generate first-strand cDNA for use in two step-PCR.</p>
[BEFORE_START]
<p>Mix and briefly centrifuge all reagents after thawing, keep on ice.</p>
[STEPS]
?.
?.
?.
?.
?. | [] |
87,407 | LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation | 1 | dx.doi.org/10.17504/protocols.io.q26g7p17qgwz/v1 | https://www.protocols.io/view/lrrk2-rckw-mli-2-e11-darpin-cryo-em-sample-prepara-czkpx4vn | Marta Sanz Murillo, Andres Leschziner | TITLE: LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation
AUTHORS: Marta Sanz Murillo, Andres Leschziner
[DESCRIPTION]
Protocol used to prepare LRRK2-RCKW: DARPin:MLi-2 complex and cryo-EM grid preparation.
[BEFORE_START]
Make
[STEPS]
SECTION: Protein purification and buffer exchange
1. His6-Z-TEV-LRRK2-RCKW ... | ["[Protein purification and buffer exchange] His6-Z-TEV-LRRK2-RCKW was expressed and purified as described in a previous protocol.", "[E11 DARPin purification] E11 DARPin purified as described in the next protocol", "[cryo-EM sample preparation] We used UltraAuFoil Holey Gold 2/2 200 mesh grids and plasma cleaning them... |
67,440 | WaterBath | 1 | null | https://www.protocols.io/view/waterbath-cd4qs8vw | bibewih | TITLE: WaterBath
AUTHORS: bibewih
[DESCRIPTION]
Instructions for creating a mock coastal environment using laboratory equipment and consumer products. This protocol covers the modification of the water bath with lights, heating and cooling. Electronic setup of controlling these heating and cooling systems are covere... | ["[Heating pads] Turn water bath on its back to gain access to bottom panel. Remove bottom panel and insulation from underneath water bath to gain access to heating pad.", "[Heating pads] Unscrew and thermometer from metal basin and peel off existing heating pad.", "[Heating pads] Attach 3d printer heating pads to meta... |
null | null | null | dx.doi.org/10.17504/protocols.io.rw8d7hw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is to fix freshly collected plant tissue for serial sectioning. Although this protocol is similar to ones used for <em>in situ</em> mRNA hybridization, it should not be used for that purpose as it is not nearly careful/gently enough. This protocol has been teste... | [] |
43,716 | Validating TBMs by Fluorescence Polarization (FP) | 5 | dx.doi.org/10.17504/protocols.io.bnxcmfiw | https://www.protocols.io/view/validating-tbms-by-fluorescence-polarization-fp-bnxcmfiw | Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler | TITLE: Validating TBMs by Fluorescence Polarization (FP)
AUTHORS: Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is part of a collection: </span><a href="https://www.protocols.io/private/DA5F6F33163011EBAE360A58A9FEAC... | ["[3.2.2 Purification of Tankyrase ARCs]\nA similar procedure is followed for the purification of all tankyrase ARCs. All buffers are ice-cold, and work is performed at 4 °C or on ice throughout. In the first step, the protein is affinity-purified by immobilized metal affinity chromatography (IMAC) using a Ni2+ affinit... |
27,604 | Automated Tissue Fixation with 10% NBF (Leica) | 1 | dx.doi.org/10.17504/protocols.io.67uhhnw | https://www.protocols.io/view/automated-tissue-fixation-with-10-nbf-leica-67uhhnw | Donna Skinner, Teri Bowman, Jon Aster | TITLE: Automated Tissue Fixation with 10% NBF (Leica)
AUTHORS: Donna Skinner, Teri Bowman, Jon Aster
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Human tissue specimens are frequently formalin-fixed and paraffin-embedded (FFPE) for histological staining, pathological review, and archival purpose... | ["[Automated Tissue Fixation with 10% NBF (Leica)]\nFormalin Short ProgramStation/ReagentTimeTempP/V10% NBF00:12 min40Y70% Ethyl Alcohol00:15 min40Y95% Ethyl Alcohol00:15 min40Y95% Ethyl Alcohol00:20 min40Y100% Ethyl Alcohol00:20 min40Y100% Ethyl Alcohol00:20 min40YXylene00:20 min40YXylene00:30 min40YParaffin Wax 100:0... |
48,167 | Neuron Image Analysis: From raw pics to .csv file | 5 | null | https://www.protocols.io/view/neuron-image-analysis-from-raw-pics-to-csv-file-btafnibn | Jesse Cohn | TITLE: Neuron Image Analysis: From raw pics to .csv file
AUTHORS: Jesse Cohn
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the pipeline I've been using to analyze images from the InCell of the Kampmann i3N-CRISPRi cell lines. The broad overall steps are:</div><div class = "... | ["[Cleaning up the folder]\nImage your plates with the \"JCohn_20x_Neurons_2.xaqp\" acquisition protocol on the InCell. This should produce a Brightfield, Red, and Green image channel for each image field, with 12 image fields per well (3 rows x 4 columns, captured in a Horizontal Serpentine fashion) of a 96-well plate... |
55,377 | Protocol 3D Gait Analysis using Treadmill Approach (CAREN) - MUMC+ | 1 | dx.doi.org/10.17504/protocols.io.b2brqam6 | https://www.protocols.io/view/protocol-3d-gait-analysis-using-treadmill-approach-b2brqam6 | Rachel Senden, Rik Marcellis, Paul Willems, Jeroen Vermeulen, Adhiambo Witlox, Kenneth Meijer | TITLE: Protocol 3D Gait Analysis using Treadmill Approach (CAREN) - MUMC+
AUTHORS: Rachel Senden, Rik Marcellis, Paul Willems, Jeroen Vermeulen, Adhiambo Witlox, Kenneth Meijer
[DESCRIPTION]
This protocol describes the steps that are conducted when performing a treadmill based 3D gait analysis at MUMC+.
[STE... | ["[Description 3D gait analysis set-up] The treadmill approach for 3D gait analysis involves walking at the Computer Assisted Rehabilitation Environment (CAREN, Motek Medical BV, Amsterdam, The Netherlands). \n\nThe CAREN is a rehabilitation system, using an instrumented dual belt treadmill for gait and balance trainin... |
28,410 | Volatile anesthetics versus total intravenous anesthesia for patients undergoing coronary artery bypass grafting: a protocol for an updated meta-analysis and trial sequential analysis | null | dx.doi.org/10.17504/protocols.io.7y2hpye | null | Xue-feng Jiao, Xue-mei Lin, Xiao-feng Ni, Hai-long Li, Chuan Zhang, Chun-song Yang, Hao-xin Song, Qiu-sha Yi, Ling-li Zhang | TITLE: Volatile anesthetics versus total intravenous anesthesia for patients undergoing coronary artery bypass grafting: a protocol for an updated meta-analysis and trial sequential analysis
AUTHORS: Xue-feng Jiao, Xue-mei Lin, Xiao-feng Ni, Hai-long Li, Chuan Zhang, Chun-song Yang, Hao-xin Song, Qiu-sha Yi, Ling-li Z... | ["[Search Strategy]\nWe searched Pubmed, Embase(Ovid), the Cochrane Library, and three Chinese databases: China Knowledge Resource Integrated Database (CNKI), VIP Database and Wanfang Database (until June 2019). We also searched ongoing clinical trials databases such as “http://clinicaltrials.gov” and “http://www.contr... |
49,069 | Protocolo do experimento - CPBL/MA/FGAD (Projeto: Elaborar seminário de Biodiversidade) | 1 | dx.doi.org/10.17504/protocols.io.bt6mnrc6 | https://www.protocols.io/view/protocolo-do-experimento-cpbl-ma-fgad-projeto-elab-bt6mnrc6 | Geiser Chalco Challco | TITLE: Protocolo do experimento - CPBL/MA/FGAD (Projeto: Elaborar seminário de Biodiversidade)
AUTHORS: Geiser Chalco Challco
[DESCRIPTION]
Main protocol in portugues used to conduct a quasi-experimental study of group formation of high performance in Collaborative-Learning-Projects with Agile Methods
[STEPS]
SECTIO... | ["[Pré-teste] Aplicar um questionário para medir o conhecimento prévio do estudante no assunto de Biodiversidade.\nO questionário será disponibilizado através do microsoft forms, com 15 questões de múltipla escolha (com 5 alternativas em cada questão). O questionário será acerca de Biodiversidade (tema do seminário) e ... |
null | null | null | dx.doi.org/10.17504/protocols.io.hf3b3qn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
73,193 | Morphometric study of the lumbar vertebrae in dried anatomical collections | 1 | dx.doi.org/10.17504/protocols.io.ewov1o9yklr2/v1 | https://www.protocols.io/view/morphometric-study-of-the-lumbar-vertebrae-in-drie-cjqhumt6 | Mangala M. Pai | TITLE: Morphometric study of the lumbar vertebrae in dried anatomical collections
AUTHORS: Mangala M. Pai
[DESCRIPTION]
Background: The objective of this anatomical study was to perform the morphometry of dried lumbar vertebrae in human cadavers.
Methods: This study utilized 200 adult human cadaveric dried lumbar ver... | ["[Morphometric study of the lumbar vertebrae in dried anatomical collections] Knowledge gap identified\nConsidering the ethnic and racial variations, which is present over the widespread geographical regions, which have influences over the lumbar vertebrae dimensions. It will be of paramount importance to conduct a st... |
28,094 | Detection of Klebsiella pneumoniae and closely related species by real-time PCR with the ZKIR system | null | dx.doi.org/10.17504/protocols.io.7n6hmhe | null | Elodie Barbier, Carla Rodrigues, Geraldine Depret, Virginie Passet, Laurent Gal, Pascal Piveteau, Sylvain Brisse | TITLE: Detection of Klebsiella pneumoniae and closely related species by real-time PCR with the ZKIR system
AUTHORS: Elodie Barbier, Carla Rodrigues, Geraldine Depret, Virginie Passet, Laurent Gal, Pascal Piveteau, Sylvain Brisse
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-st... | ["qPCR mix preparation (final volume 20 µl) ABC1Mix reagentsVolume per well (µl)Final concentration2Takyon™ ROX SYBR® MasterMix 2X103Forward (3 µM)2300 nM4Reverse (3 µM)2300 nM5T4 gp32 (optional)0.512.5 µg/ml6PCR grade waterQS 17.5 µl7DNA template2.5\nABC1Mix reagentsVolume per well (µl)Final concentration2Takyon™ ROX... |
null | null | null | dx.doi.org/10.17504/protocols.io.h94b98w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p>Adapted from the original publication Stein, J (ED.) 1973. Handbook of Phycological methods. Culture methods and growth measurements. Cambridge University P... | [] |
37,085 | Tissue Sample Preparation for LC-MS Analysis | 1 | dx.doi.org/10.17504/protocols.io.bgf5jtq6 | https://www.protocols.io/view/tissue-sample-preparation-for-lc-ms-analysis-bgf5jtq6 | Joanne Chan, Ruiqi Jian, Lihua Jiang | TITLE: Tissue Sample Preparation for LC-MS Analysis
AUTHORS: Joanne Chan, Ruiqi Jian, Lihua Jiang
[DESCRIPTION]
This protocol describes the procedure for protein extraction, enzymatic digestion, sample cleanup an dTMT labeling for LC-MS analysis. Proper sample preparation and clean-up are extremely important to ensu... | ["[Protein Lysate Extraction] Place the tissue chunk onto a cell culture plate on ice and mince with disposable scalpels to the best of your abilities.", "[Protein Lysate Extraction] Add 500 µL lysis buffer (6 Molarity (M) GdmCl, 10 Milimolar (mM) TCEP, 40 Milimolar (mM) CAA, 100 Milimolar (mM) Tris pH 8.5) to the min... |
71,537 | PCR cleanup and size selection with magnetic beads | 4 | dx.doi.org/10.17504/protocols.io.36wgqj45xvk5/v3 | https://www.protocols.io/view/pcr-cleanup-and-size-selection-with-magnetic-beads-ch4rt8v6 | Dominik Buchner | TITLE: PCR cleanup and size selection with magnetic beads
AUTHORS: Dominik Buchner
[DESCRIPTION]
This protocol describes how to clean up PCR products or DNA extracts and perform a size selection with carboxylated-magnetic beads and a PEG-NaCl buffer. It can also be used for volume reduction of a sample or for buffer e... | ["Shake the cleanup solution until the beads are homogeneously resuspended", "Add 30 µL and 32 µL to a 250 µL U-bottom assay plate", "Add 10 µL of sample.", "To bind the DNA to the beads shake at", "Place the plate on a magnet to pellet the beads for 2 min", "Discard the supernatant by pipetting", "With the plate stil... |
85,918 | Total carbohydrate content for coral samples | 4 | dx.doi.org/10.17504/protocols.io.36wgq3j4xlk5/v1 | https://www.protocols.io/view/total-carbohydrate-content-for-coral-samples-cx56xq9e | Keyla Plichon, Paola Furla | TITLE: Total carbohydrate content for coral samples
AUTHORS: Keyla Plichon, Paola Furla
[DESCRIPTION]
This protocol aims to quantify total carbohydrate content from a cytosolic extraction. It has been optimized for carbohydrate analysis from coral species. Here, the cytosolic extraction containing proteins and carbohy... | ["[Carbohydrate quantification in a 96-well plate] Carbohydrate standard for colorimetric detection.\nAdd 0, 1, 2, 4, 6, 8, 10, and 12 μL of the glucose stock solution directly into the 96-well plate. Bring the volume to 30 μL. Each well will, therefore, contain 0 (blank), 2, 4, 8, 12, 16, 20, and 24 μg.", "[Carbohydra... |
37,960 | HTAPP_Dissociation of human primary lung cancer resection to a single-cell suspension for single-cell RNA-seq | 1 | dx.doi.org/10.17504/protocols.io.bhbgj2jw | https://www.protocols.io/view/htapp-dissociation-of-human-primary-lung-cancer-re-bhbgj2jw | Michal Slyper, Avinash Waghray, Julia Waldman, Isaac Wakiro, Mei-Ju Su, Sébastien Vigneau, Asaf Rotem, Bruce Johnson, Orit Rozenblatt-Rosen, Aviv Regev | TITLE: HTAPP_Dissociation of human primary lung cancer resection to a single-cell suspension for single-cell RNA-seq
AUTHORS: Michal Slyper, Avinash Waghray, Julia Waldman, Isaac Wakiro, Mei-Ju Su, Sébastien Vigneau, Asaf Rotem, Bruce Johnson, Orit Rozenblatt-Rosen, Aviv Regev
[DESCRIPTION]
<div class = "text-blocks">... | ["[Sample Description and Allocation]\nReport sample processing information.\n[Wet Ice]\nSample ID:\nDate:Media Used for Transportation:\nPerson Processing:", "[Sample Description and Allocation]\nTransfer sample to a Petri dish with cold PBS (or HBSS without phenol red) kept on ice to better visualize its composition.... |
28,528 | Flow CAM 8000 Operation Protocol/Sample | null | dx.doi.org/10.17504/protocols.io.74qhqvw | null | Brittany Zepernick, Steven Wilhelm | TITLE: Flow CAM 8000 Operation Protocol/Sample
AUTHORS: Brittany Zepernick, Steven Wilhelm
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The FlowCAM is a useful tool for morphological and physiological parameter analysis as well as identification of plankton. With over 30 different measurements av... | ["[Power ON]\nTurn the fan ON using the switch on the back of the FlowCAM. Press the circular power button on the top right. Power on the monitor.* Always shut down FlowCAM when you have finished using it.", "[Instrument Set-up]\nYou must consider the sample/community you are analyzing when selecting the objective, flo... |
103,026 | Immunostaining and quantification of intracellular accessible cholesterol using ALOD4-mNeon in Human Fibroblasts | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj5prlx1/v1 | https://www.protocols.io/view/immunostaining-and-quantification-of-intracellular-dgus3wwe | Sreeja V Nair, Ebsy Jaimon, Suzanne R Pfeffer | TITLE: Immunostaining and quantification of intracellular accessible cholesterol using ALOD4-mNeon in Human Fibroblasts
AUTHORS: Sreeja V Nair, Ebsy Jaimon, Suzanne R Pfeffer
[DESCRIPTION]
Here we present a protocol for quantitative immunostaining of intracellular accessible cholesterol using ALOD4-mNeon in human fibr... | ["[ALOD4 Staining and image acquisition] This section describes plating and staining of cells and image acquisition.", "[ALOD4 Staining and image acquisition] Seed 0.2 X 106 cells onto 3-4 collagen-coated 12 mm coverslips, in each well of a six-well plate", "[ALOD4 Staining and image acquisition] 16-18 hr after plating... |
91,503 | Protocol for Synthesis and Preparation of Metal-Organic Framework (MOF) Nanoparticles (10ml Total Volume) | 4 | dx.doi.org/10.17504/protocols.io.14egn3jkyl5d/v1 | https://www.protocols.io/view/protocol-for-synthesis-and-preparation-of-metal-or-c5kpy4vn | Hussain Zubair | TITLE: Protocol for Synthesis and Preparation of Metal-Organic Framework (MOF) Nanoparticles (10ml Total Volume)
AUTHORS: Hussain Zubair
[DESCRIPTION]
This protocol delineates a comprehensive method for synthesizing and preparing Metal-Organic Framework (MOF) nanoparticles, incorporating a STING agonist (2'3'-cGAMP). ... | ["[Reagents] Dimethylformamide (DMF)\nBenzoic Acid\nZirconium(IV) Chloride Octahydrate (ZrCl4·8H2O)\nMeso-Tetraphenylporphine (TPP, C44H30N4O8)\nDimethyl Silicone Oil\nNitrogen Dioxide (NO2)\nSTING Agonist (2'3'-cGAMP, Cyclic GMP-AMP Sodium Salt)\nPhosphate-Buffered Saline (PBS)", "[Step 1: MOF Synthesis] Dissolve 0.03... |
98,785 | Singleplex Assay for Function Measurements | 0 | dx.doi.org/10.17504/protocols.io.dm6gpzwx8lzp/v1 | https://www.protocols.io/view/singleplex-assay-for-function-measurements-dcp92vr6 | David Ross | TITLE: Singleplex Assay for Function Measurements
AUTHORS: David Ross
[DESCRIPTION]
This protocol outlines an assay for measuring the function of plasmid variants in singleplex.
The inputs include separate E. coli glycerol stocks for each variant. The protocol begins with several growths which convert the separate g... | ["[Culture Preparation] For each variant to be tested, fill a 15 mL snap-cap culture tube with 5 mL of M9 media.\nUse a scraping from the glycerol stock for each clonal variant and place into its culture tube.", "[Prepare the automation system or liquid handler] Load required reagents (M9 media, additives, PBS, etc.) a... |
52,622 | Cell-based Tau seeding assay | 1 | dx.doi.org/10.17504/protocols.io.x54v9jnrzg3e/v1 | https://www.protocols.io/view/cell-based-tau-seeding-assay-bxmnpk5e | Patricia Yuste-Checa | TITLE: Cell-based Tau seeding assay
AUTHORS: Patricia Yuste-Checa
[DESCRIPTION]
This protocol details the procedure of cell-based Tau seeding assay.
[GUIDELINES]
Banning, C. et al. A flow cytometry-based FRET assay to identify and analyse proteinprotein interactions in living cells. PLoS One 5, e9344, doi:10.1371/jou... | ["Plate 100,000 cells per well of HEK293T FRET reporter cell line (TauRD-YT, FLTau-YT or commercial cell line Tau RD P301S FRET Biosensor (ATCC, CRL- 3275)) into a 12-well plate.", "On the next day, seed aggregation in cells with Tau aggregates.", "Seeding with transfection reagent:\n\nPrepare lipofectamine mix for n+1... |
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