id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
43,639 | Fungal DNA Extraction from Fungal Plate | 3 | dx.doi.org/10.17504/protocols.io.bnuxmexn | https://www.protocols.io/view/fungal-dna-extraction-from-fungal-plate-bnuxmexn | You Li, Demian F Gomez | TITLE: Fungal DNA Extraction from Fungal Plate
AUTHORS: You Li, Demian F Gomez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to do fungal DNA extraction from fungal plate. </div><div class = "text-block"><span>This protocol is part of the Bark Beetle Mycobiome (BBM) Res... | [] |
82,833 | MicroMPN: Software for Automating Most Probable Number Estimates from Laboratory Microplates | 5 | dx.doi.org/10.17504/protocols.io.81wgbymenvpk/v1 | https://www.protocols.click/view/micrompn-software-for-automating-most-probable-num-cu5rwy56 | Adam R Rivers, Karla Franco Meléndez | TITLE: MicroMPN: Software for Automating Most Probable Number Estimates from Laboratory Microplates
AUTHORS: Adam R Rivers, Karla Franco Meléndez
[DESCRIPTION]
We developed a command-line Python package named "MicroMPN" to enable the automation of most probable number (MPN) value estimations from microplates of differ... | ["[Example of Input file 1: namefile.csv] To run MicroMPN, users need an input CSV file with RFU data.\n\nThe column headers must include the following identifiers:\nplate_unique\nplate_well\nrfu\n\n*Additional column names in the input file will be ignored by MicroMPN.\n\nAs an example of a CSV file with relative fluo... |
28,517 | NEB Monarch® RNA Cleanup Kit | 1 | null | https://www.protocols.io/view/neb-monarch-rna-cleanup-kit-74dhqs6 | New England Biolabs | TITLE: NEB Monarch® RNA Cleanup Kit
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is for the Monarch RNA Cleanup Kits (NEB #’s </span><a href="https://international.neb.com/products/t2030-monarch-rna-cleanup-kit-10-ug" style = "text-decoration:unde... | ["Add to the 50 μl sample. A starting sample volume of 50 μl is recommended. For smaller samples, nuclease-free water can be used to adjust the volume. For samples larger than 50 μl, scale buffer volumes accordingly. Samples with a starting volume > 150 μl will require reloading of the column during Step 3.\n[RNA Clea... |
63,904 | Vissentials Max BHB Canada-Uses, Side Effects, and More | 3 | dx.doi.org/10.17504/protocols.io.5jyl89k48v2w/v1 | https://www.protocols.io/view/vissentials-max-bhb-canada-uses-side-effects-and-m-cam8sc9w | Vissentials Max | TITLE: Vissentials Max BHB Canada-Uses, Side Effects, and More
AUTHORS: Vissentials Max
[DESCRIPTION]
Vissentials Max BHB weight loss supplement can help you shed weight fast. The pills boost metabolism. Numerous people have used the keto supplement and shed weight in a brief amount of period of.
[STEPS] | [] |
51,789 | p1 22/7 | 1 | null | https://www.protocols.io/view/p1-22-7-bwtmpek6 | Mariia Guliakina II | TITLE: p1 22/7
AUTHORS: Mariia Guliakina II
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">test</div></div>
[STEPS]
?. test | ["test"] |
null | null | null | dx.doi.org/10.17504/protocols.io.jhscj6e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Sequencing library construction, exome capture, sequencing, and standard data analyses for the affected children in this family was performed by Sengenics. Exome capturing and enrichment was carried out using SureSelect All Exon V5 kit (Agilent Technologies, Santa Clara, CA, ... | [] |
49,011 | How to check the integrity of a set of files uploaded to AWS S3 | 5 | null | https://www.protocols.io/view/how-to-check-the-integrity-of-a-set-of-files-uploa-bt4tnqwn | Sonia García-Ruiz | TITLE: How to check the integrity of a set of files uploaded to AWS S3
AUTHORS: Sonia García-Ruiz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol contains the instructions to verify the integrity of any small or large file uploaded to AWS S3. </div><div class = "text-block">This protoc... | ["Clone the s3md5 repo:$ git clone https://github.com/antespi/s3md5.git", "Grant execution access to the s3md5 script file.$ chmod 755 ./s3md5/s3md5", "Clone the aws-s3-integrity-check repo:$ git clone https://github.com/SoniaRuiz/aws-s3-integrity-check.git", "Move the 's3md5' folder within the 'aws-s3-integrity-check'... |
null | null | null | dx.doi.org/10.17504/protocols.io.d7u9nv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Goal: Identify 16S rRNA gene metagenomic fragments and create assembled full-length 16S rRNA sequences from fragments.<br />Note 1 - As is this case with most bioinformatic processes, this is one of many possible methods, but has had successful results in the past<br />Note 2 - ... | [] |
97,564 | Autophagosome live cell imaging | 0 | dx.doi.org/10.17504/protocols.io.5qpvoknobl4o/v1 | https://www.protocols.io/view/autophagosome-live-cell-imaging-dbh42j8w | Dan Tudorica, bishal.penn | TITLE: Autophagosome live cell imaging
AUTHORS: Dan Tudorica, bishal.penn
[DESCRIPTION]
For quantification of PINK-PARKIN mitophagy
[STEPS]
SECTION: Cell Culture
1. Seed Parkin-expressing HeLa cells for ~80% confluence on day of transfection on tissue-culture coated glass slides, suitable for use on a confocal micros... | ["[Cell Culture] Seed Parkin-expressing HeLa cells for ~80% confluence on day of transfection on tissue-culture coated glass slides, suitable for use on a confocal microscope.", "[Cell Culture] On day of transfection, transfect pCIG2-LAMP1-mNeon Green, pHaloTag-LC3B, and an autophagy regulating protein tagged with mche... |
36,154 | CLIAHUB Automated RNA Extraction & RT-PCR Protocol V2 | 1 | dx.doi.org/10.17504/protocols.io.bfi2jkge | https://www.protocols.io/view/cliahub-automated-rna-extraction-amp-rt-pcr-protoc-bfi2jkge | Amy Lyden, Emily Crawford, Vida Ahyong, Manu Vanaerschot, Paula Hayakawa Serpa, Preethi Raghavan, Joana Cabrera, Michael Borja, Spyros Darmanis, Eric Chow, Joseph Derisi | TITLE: CLIAHUB Automated RNA Extraction & RT-PCR Protocol V2
AUTHORS: Amy Lyden, Emily Crawford, Vida Ahyong, Manu Vanaerschot, Paula Hayakawa Serpa, Preethi Raghavan, Joana Cabrera, Michael Borja, Spyros Darmanis, Eric Chow, Joseph Derisi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Github: (to ... | ["[Reagent Plate Preparation]\nAll reagent plates should be barcoded. Record barcode of the plate and lot number of the reagents used in each plate (ie record the lot numbers of DNAse, DNAse Digestion Buffer and water used to make DNAse master mix). It is also recommended that you label the reagents with a human readab... |
16,054 | Changes in spinal stiffness with chronic thoracic pain: correlation with pain and muscle activity | null | dx.doi.org/10.17504/protocols.io.twwepfe | null | Isabelle Pagé, François Nougarou, Arnaud Lardon, Martin Descarreaux | TITLE: Changes in spinal stiffness with chronic thoracic pain: correlation with pain and muscle activity
AUTHORS: Isabelle Pagé, François Nougarou, Arnaud Lardon, Martin Descarreaux
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The main goal of this project is to assess the reliability of thoracic... | [] |
47,424 | Manual H&E Staining | 1 | dx.doi.org/10.17504/protocols.io.bsi8nchw | https://www.protocols.io/view/manual-h-amp-e-staining-bsi8nchw | Jia Ren Lin | TITLE: Manual H&E Staining
AUTHORS: Jia Ren Lin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of fixed tissue specimens. This procedure establishes a consistent process for the manual preparation of H&E-stained ... | ["[Post-CyCIF Decoverslipping]\nAfter the final imaging step of CyCIF (or IF), soak slides in 1X PBS for and gently detach coverslips from slides using slide forceps.\n0 Room temperature", "[Manual H&E Staining]\nRemove slides and place on the stain box. Add between and Hematoxylin solution and let incubate for .\n2... |
85,279 | Subjective Data Models in Bioinformatics - Interview-based personal data model elicitation | 1 | dx.doi.org/10.17504/protocols.io.q26g7p4r1gwz/v1 | https://www.protocols.io/view/subjective-data-models-in-bioinformatics-interview-cxh7xj9n | Yo Yehudi, Caroline Jay, lukasnoehrer, Carole Goble | TITLE: Subjective Data Models in Bioinformatics - Interview-based personal data model elicitation
AUTHORS: Yo Yehudi, Caroline Jay, lukasnoehrer, Carole Goble
[DESCRIPTION]
Biological science produces large amounts of data in a variety of formats,
which necessitates the use of computational tools to process, integrate... | ["[Initial experiment set-up] Start by preparing the materials you'll need for your interviews. \n\nGather the following items:", "[Initial experiment set-up] Print biological files for the file-mapping task\nThese could be the pre-supplied files (GFF and FASTA for Homo sapiens and Drosophila melanogaster), or other fi... |
57,796 | Nucleofection of hPSCs | 4 | dx.doi.org/10.17504/protocols.io.b4pcqviw | https://www.protocols.io/view/nucleofection-of-hpscs-b4pcqviw | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Nucleofection of hPSCs
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the standard procedure for the delivery of plasmids, mRNA or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection.
General notes
Throu... | ["For each nucleofection, prepare 20 µl nucleofection solution by mixing 16.4 µl solution I and 3.6 µl supplement.", "Re-suspend cell pellets in nucleofection solution. Mix using P1000 or P200 tips to ensure single cell suspension.", "Transfer 20 µl cell suspension to the microcentrifuge tube containing the materials t... |
106,672 | Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Stool | 4 | dx.doi.org/10.17504/protocols.io.rm7vzbyyxvx1/v5 | https://www.protocols.io/view/direct-detection-of-poliovirus-and-nanopore-sequen-dkeq4tdw | Alex Shaw, Catherine Troman, Joyce Akello, Erika Bujaki, Manasi Majumdar, Shannon Fitz, Ben Bellekom, Aine OToole, c.ansley, rachel.colquhoun, arshady, khurshida, alammu, Andrew Rambaut, Javier Martin, Nick Grassly | TITLE: Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Stool
AUTHORS: Alex Shaw, Catherine Troman, Joyce Akello, Erika Bujaki, Manasi Majumdar, Shannon Fitz, Ben Bellekom, Aine OToole, c.ansley, rachel.colquhoun, arshady, khurshida, alammu, Andrew Rambaut, Javier Martin, Nick Grassly
[DESCRIPTION]
This... | ["[First Round PCR (semi-nest)] Prepare a master mix using the reaction volumes detailed in the table below for the number of samples you have plus negative controls. The reaction mix and SSIII enzyme are provided in \n\n\nForward primer: Y7 [GGGTTTGTGTCAGCCTGTAATGA] \n\nReverse Primers: Cre [TCAATACGGTGTTTGCTCTTGAAC... |
20,314 | Psychometric evaluation of the Korean version of the Hepatitis B Quality of Life Questionnaire | null | dx.doi.org/10.17504/protocols.io.x32fqqe | null | Yeonsoo Jang, Sang Hoon Ahn, Kyunghwa Lee, Jiyeon Lee, Jeong Hyun Kim | TITLE: Psychometric evaluation of the Korean version of the Hepatitis B Quality of Life Questionnaire
AUTHORS: Yeonsoo Jang, Sang Hoon Ahn, Kyunghwa Lee, Jiyeon Lee, Jeong Hyun Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Purpose: The purpose of this study was to conduct a psychometric evalua... | [] |
25,116 | Cellular protein extraction and Western blotting using dry transfer (iBlot system) | null | dx.doi.org/10.17504/protocols.io.4r4gv8w | null | Ralitsa R. Madsen | TITLE: Cellular protein extraction and Western blotting using dry transfer (iBlot system)
AUTHORS: Ralitsa R. Madsen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A generic protein extraction and Western blotting protocol, allowing both for total cell lysis or cytoplasmic protein extraction only. ... | ["[Cell lysis and protein extraction]\nPre-chill the benchtop microcentrifuge to .Prepare cell scraper and a beaker with PBS - this will be used to rinse the cell scraper in between processing of different samples.\n4 °C", "[Cell lysis and protein extraction]\nAliquot 10 ml of stock lysis buffer and dissolve the requi... |
null | null | null | dx.doi.org/10.17504/protocols.io.jz5cp86 | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p>This protocol uses an adapted version of Omega Bio-Tek’s E.Z.N.A. Bacterial DNA Kit. In new versions of the kit BTL Buffer has been renamed TL Buffer, BDL Buffer has been renamed BL Buffer, and HB Buffer has been renamed HBC Buffer.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.qf7dtrn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Melanophores (also called melanocytes) are chromatophores (pigment-containing epithelial cells) that contain black or dark brown pigment granules. The aggregation or dispersal of these cells is under hormonal or nervous system control; these responses permit the animal to dis... | [] |
66,317 | https://www.facebook.com/Diaetoxil600mg/ | 1 | dx.doi.org/10.17504/protocols.io.3byl4b4kjvo5/v1 | https://www.protocols.io/view/https-www-facebook-com-diaetoxil600mg-cczmsx46 | cristianoballor | TITLE: https://www.facebook.com/Diaetoxil600mg/
AUTHORS: cristianoballor
[DESCRIPTION]
Diaetoxil 600mg: Contrairement à d'autres helpers à l'amincissement, ces cases ont un effet polyvalent sur l'organisme, de sorte qu'une réduction de poids souhaitée peut être soutenue de manière sturdy et surtout permanente. Posit... | ["[https://www.facebook.com/Diaetoxil600mg/]"] |
101,628 | Preparation of Tissue Samples for DNA Extraction and Copy Number Analysis by iDNA Technologies | 1 | null | https://www.protocols.io/view/preparation-of-tissue-samples-for-dna-extraction-a-dfg43jyw | Lynn Doran | TITLE: Preparation of Tissue Samples for DNA Extraction and Copy Number Analysis by iDNA Technologies
AUTHORS: Lynn Doran
[DESCRIPTION]
Preparation of leaf tissue for shipment to iDNA Genetics for DNA extraction and copy number analysis.
NOTE: TOBACCO TISSUE IS REGULATED AS DRUGS AND ALCOHOL. iDNA maintains speci... | ["[Get a Quote] Email iDNA Technologies for a quote. Include the number of samples to be tested, which genes you want copy number analysis on, and which species the samples are from. \n\nInclude one sample with a known copy number of 2 as an internal reference standard for each assay (gene).", "[Get a Quote] When you'... |
86,465 | DNeasy PowerSoil Pro Kit modification for soil fungal community barcoding | 4 | dx.doi.org/10.17504/protocols.io.3byl4qq78vo5/v1 | https://www.protocols.io/view/dneasy-powersoil-pro-kit-modification-for-soil-fun-cyn9xvh6 | Tyler W. d'Entremont, Stephanie N. Kivlin | TITLE: DNeasy PowerSoil Pro Kit modification for soil fungal community barcoding
AUTHORS: Tyler W. d'Entremont, Stephanie N. Kivlin
[DESCRIPTION]
Here we provide a modified protocol for use with the Qiagen PowerSoil Pro Kit that has been used with great success for obtaining fungal DNA from field collected soil sample... | ["Centrifuge the PowerBead Pro Tubes briefly to ensure contents have settled to the bottom.", "Weigh out 250 mg of soil and add to the Powerbead Pro Tube. Add 750 µL of Solution CD1 and 50 µL of Solution ATL (ordered seperately from the kit contents). Vortex to homogenize.", "Place samples in a Biospec Mini-beadbeater ... |
104,147 | Spore based infection assay on Pinus sylvestris seedlings with Diplodia sapinea | 0 | null | https://www.protocols.io/view/spore-based-infection-assay-on-pinus-sylvestris-se-dhxt37nn | Anne Oostlander, Laura Brodde, Miriam von Bargen, Rasmus Enderle, Marco Leiterholt, Dagmar Trautmann, Malin Elfstrand, Jan Stenlid, André Fleißner | TITLE: Spore based infection assay on Pinus sylvestris seedlings with Diplodia sapinea
AUTHORS: Anne Oostlander, Laura Brodde, Miriam von Bargen, Rasmus Enderle, Marco Leiterholt, Dagmar Trautmann, Malin Elfstrand, Jan Stenlid, André Fleißner
[DESCRIPTION]
This protocol describes a spore based method for assessing Dip... | ["[Plant Material and Greenhouse Conditions] Use 2 year old container seedlings of Scots pine (Pinus sylvestris).", "[Plant Material and Greenhouse Conditions] Ensure seedlings have no visible symptoms and have undergone two annual cycles.", "[Plant Material and Greenhouse Conditions] Apply the last fungicide treatment... |
43,676 | Gel Electrophoresis 1- Casting a gel in the Bento Lab gel box | 3 | null | https://www.protocols.io/view/gel-electrophoresis-1-casting-a-gel-in-the-bento-l-bnv4me8w | TITLE: Gel Electrophoresis 1- Casting a gel in the Bento Lab gel box
AUTHORS:
[STEPS] | [] | |
25,151 | Upper limb superficial venous percussion as a sign of anatomical location and venous permeability | null | dx.doi.org/10.17504/protocols.io.4s7gwhn | null | Pedro Coelho N Diogenes, Aline Naiara Azevedo da Silva, Fausto Pierdoná Guzen, Marco Aurelio de Moura Freire, José Rodolfo Lopes de Paiva Cavalcanti | TITLE: Upper limb superficial venous percussion as a sign of anatomical location and venous permeability
AUTHORS: Pedro Coelho N Diogenes, Aline Naiara Azevedo da Silva, Fausto Pierdoná Guzen, Marco Aurelio de Moura Freire, José Rodolfo Lopes de Paiva Cavalcanti
[STEPS]
?. The upper limb being examined was garrotted.
... | ["The upper limb being examined was garrotted.", "The anatomical sites of the main four superficial venous segments of the upper limbs was examined separately (cephalic vein in forearm, cephalic vein in arm, basilic vein in forearm and basilic vein in the arm).", "Each segment was struck distally with forefinger of the... |
28,869 | Western Blot (semi-dry) | null | dx.doi.org/10.17504/protocols.io.8fdhti6 | null | Anna Behle, Anika Wiegard | TITLE: Western Blot (semi-dry)
AUTHORS: Anna Behle, Anika Wiegard
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for semi-dry Western Blot</div></div>
[STEPS]
?. [Western Blot]
Equilibrate gel in Cathode buffer for 30 minutes (no shaking)
?. [Western Blot]
Activate PCDF-Membrane for ca. 2... | ["[Western Blot]\nEquilibrate gel in Cathode buffer for 30 minutes (no shaking)", "[Western Blot]\nActivate PCDF-Membrane for ca. 20 seconds in MeOH, incubate for 2 minutes in dH2O (no shaking)", "[Western Blot]\nEquilibrate PCDF-Membrane for 5 minutes in Anode buffer", "[Western Blot]\nWet two Blotting papers (thickne... |
35,585 | 20X Anatomic and Layer Identification for Biocytin Filled Cells | null | dx.doi.org/10.17504/protocols.io.bey9jfz6 | null | Allen Institute for Brain Science | TITLE: 20X Anatomic and Layer Identification for Biocytin Filled Cells
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">To characterize the orientation, laminar position, and anatomical location of in vitro single cells reconstructed in mouse and human brain... | [] |
75,109 | LEGACY01: DATA AND SAMPLE SHARING | 1 | null | https://www.protocols.io/view/legacy01-data-and-sample-sharing-cmkdu4s6 | Katrina M Pollock, Calliope Dendrou | TITLE: LEGACY01: DATA AND SAMPLE SHARING
AUTHORS: Katrina M Pollock, Calliope Dendrou
[DESCRIPTION]
This protocol details data and sample sharing in an experimental medicine study of seasonal influenza vaccination responses in Lymph nodE single-cell Genomics in AnCestrY (LEGACY01).
[GUIDELINES]
DATA AND SAMPLE SHARIN... | [] |
59,159 | Insulin Tolerance Test | 1 | dx.doi.org/10.17504/protocols.io.b5zxq77n | https://www.protocols.io/view/insulin-tolerance-test-b5zxq77n | Dave Bridges, Molly C Mulcahy, JeAnna R. Redd | TITLE: Insulin Tolerance Test
AUTHORS: Dave Bridges, Molly C Mulcahy, JeAnna R. Redd
[DESCRIPTION]
Insulin tolerance tests are a standard and common method for evaluating the insulin sensitivity of an animal. In this assay, animals are fasted to normalize blood glucose and then challenged by an intraperitoneal in... | ["[Preparation] Remove food from mice for about 6h by putting them in a fresh cage. Add do not feed tag to cages, or ideally move cage to procedure room. Try to make sure that the mice are in a quiet, undisturbed temperature-controlled room with the lights on.", "[Assay] Prepare insulin syringes with 10 uL per g mouse ... |
103,114 | Culturing 3T3 Cells for Validating GEARBOCS 2.0 Constructs | 0 | dx.doi.org/10.17504/protocols.io.e6nvw18pzlmk/v1 | https://www.protocols.io/view/culturing-3t3-cells-for-validating-gearbocs-2-0-co-dgxi3xke | Justin T Savage | TITLE: Culturing 3T3 Cells for Validating GEARBOCS 2.0 Constructs
AUTHORS: Justin T Savage
[DESCRIPTION]
This protocol is for culturing Cas9 NIH/3T3 cells for the validation of GEARBOCS 2.0 and similar constructs for use in in vivo assays.
[STEPS]
1. Validation by indel sequencing in immortalized murine cell lines:
... | ["Validation by indel sequencing in immortalized murine cell lines:", "Seed Cas9 NIH/3T3 cells at 250k per well of a 6-well plate (if starting from frozen cells, you. will need to passage at least once prior to transfection. So thaw to 10cm dish, then passage to 6-well)", "24 hours after seeding, transfect Cas9 NIH/3T3... |
76,456 | DNA extraction protocol for snake genomic sequencing by using AxyPrepTM Multisource Genomic DNA Miniprep Kit | 4 | dx.doi.org/10.17504/protocols.io.n92ldp2m8l5b/v2 | https://www.protocols.io/view/dna-extraction-protocol-for-snake-genomic-sequenci-cnwgvfbw | Boyang Liu, Liangyu Cui, Zhangwen Deng, Yue Ma, Diancheng Yang, Yanan Gong, Yanchun Xu, Shuhui Yang, Song Huang | TITLE: DNA extraction protocol for snake genomic sequencing by using AxyPrepTM Multisource Genomic DNA Miniprep Kit
AUTHORS: Boyang Liu, Liangyu Cui, Zhangwen Deng, Yue Ma, Diancheng Yang, Yanan Gong, Yanchun Xu, Shuhui Yang, Song Huang
[DESCRIPTION]
Genomic DNA was extracted from the muscle of a snake using the AxyPr... | ["The following protocol is a modified DNA extraction protocol using the AxyPrepTM Multisource Genomic DNA Miniprep Kit. \n\nApproximately 10mg of snake tissue was minced into small pieces using sterilized scissors on ice.\non ice", "Add 350 µl Buffer PBS and 15 µl SDS Lysis Buffer and shake for 30s.\n30 s", "Add 150 µ... |
40,928 | Copy of Copy of Copy of ELISA for quantification of human C9 in serum or plasma. | 6 | dx.doi.org/10.17504/protocols.io.bj78krrw | https://www.protocols.io/view/copy-of-copy-of-copy-of-elisa-for-quantification-o-bj78krrw | Angel Justiz-Vaillant, Belkis Ferrer-Cosme | TITLE: Copy of Copy of Copy of ELISA for quantification of human C9 in serum or plasma.
AUTHORS: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. An anti-human C9 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbona... | ["An anti-human C9 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.", "Add 50 µl of human serum or plasma. Human C9 present in the serum or plasma binds to antibodies adsorbed into the microwells.", "The microplate is blocked with 3% non-fat milk-PBS buf... |
66,874 | Blazing Keto *IS REAL DIET* Melt Fat Fast with no Exercise! | 3 | dx.doi.org/10.17504/protocols.io.rm7vzyp85lx1/v1 | https://www.protocols.io/view/blazing-keto-is-real-diet-melt-fat-fast-with-no-ex-cdi2s4ge | g | TITLE: Blazing Keto *IS REAL DIET* Melt Fat Fast with no Exercise!
AUTHORS: g
[DESCRIPTION]
Don't bother keeping is being worried about things that you at last can have command over with this strong new formula.
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dny5fv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol described a easy procedure to purify anti-sera in small scale batch. Protein A or protein G beads can be used depending on the immunoglobulin types.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
67,243 | The Acute Effects of Gastric Electrical Stimulation on Nucleus of the Solitary Tract Neural Activity Assessed with Electrophysiological Recording | 1 | dx.doi.org/10.17504/protocols.io.4r3l2okzjv1y/v1 | https://www.protocols.io/view/the-acute-effects-of-gastric-electrical-stimulatio-cdwjs7cn | Jiayue Cao, Kun-Han Lu, Xiaokai Wang, Zhongming Liu | TITLE: The Acute Effects of Gastric Electrical Stimulation on Nucleus of the Solitary Tract Neural Activity Assessed with Electrophysiological Recording
AUTHORS: Jiayue Cao, Kun-Han Lu, Xiaokai Wang, Zhongming Liu
[DESCRIPTION]
Brief description
This protocol describes the methods used to evaluate the effects of gas... | ["[Anesthesia Procedure] Anesthetize the animal with 5% Isoflurane mixed with 100% oxygen at a flow rate of 500-1000 mL/min for 5 minutes (or till the animal loses consciousness).", "[Anesthesia Procedure] After the animal loses consciousness, deliver a subcutaneous bolus injection of 0.075 mg/kg dexmedetomidine soluti... |
92,814 | UV decontamination of reagents/buffers | 3 | dx.doi.org/10.17504/protocols.io.e6nvwdn5dlmk/v1 | https://www.protocols.io/view/uv-decontamination-of-reagents-buffers-c6vnze5e | Elena Essel, Matthias Meyer, Merlin Szymanski | TITLE: UV decontamination of reagents/buffers
AUTHORS: Elena Essel, Matthias Meyer, Merlin Szymanski
[DESCRIPTION]
Protocol for reducing DNA contamination of reagents and buffers used in the ancient DNA cleanroom by UV treatment.
[STEPS] | [] |
62,565 | Calmwave CBD Gummies - CBD Full-spectrum is Great For Your Body Cost & Buy! | 3 | dx.doi.org/10.17504/protocols.io.3byl4bxdovo5/v1 | https://www.protocols.io/view/calmwave-cbd-gummies-cbd-full-spectrum-is-great-fo-b9cdr2s6 | G G | TITLE: Calmwave CBD Gummies - CBD Full-spectrum is Great For Your Body Cost & Buy!
AUTHORS: G G
[DESCRIPTION]
This product it is not intended to diagnose, treat, cure, or prevent any disease. Not recommended for use by children under the age of 18, or if you are pregnant or breastfeeding.
[STEPS] | [] |
36,857 | Nested VP1 PCR and Nanopore Sequencing from Stool and ES Samples | 1 | dx.doi.org/10.17504/protocols.io.bf8zjrx6 | https://www.protocols.io/view/nested-vp1-pcr-and-nanopore-sequencing-from-stool-bf8zjrx6 | Alex Shaw, Manasi Majumdar, Catherine Troman, Javier Martin, Nick Grassly | TITLE: Nested VP1 PCR and Nanopore Sequencing from Stool and ES Samples
AUTHORS: Alex Shaw, Manasi Majumdar, Catherine Troman, Javier Martin, Nick Grassly
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is published in the paper "Rapid and sensitive direct detection and identific... | ["[Nested PCR First Round (PanEV)]\nNested PCR First Round (panEV primers):Prepare a Master mix using reaction volumes as detailed below, excluding forward primer and the RNA:Forward Primer: TGGCGGAACCGACTACTTTGGGTG (Arita et al. 2015)Reverse Primer: TCAATACGGTGTTTGCTCTTGAACTG (Arita et al. 2015) AB11 Reaction (µL... |
51,157 | Nissl Staining and Imaging of Mouse Brain Tissue for Slide-seq Registration | 4 | dx.doi.org/10.17504/protocols.io.bv7vn9n6 | https://www.protocols.io/view/nissl-staining-and-imaging-of-mouse-brain-tissue-f-bv7vn9n6 | Karol Balderrama, Chuck Vanderburg, Evan Murray | TITLE: Nissl Staining and Imaging of Mouse Brain Tissue for Slide-seq Registration
AUTHORS: Karol Balderrama, Chuck Vanderburg, Evan Murray
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol that describes the steps used for staining adjacent tissue sections when using Slide-seq on m... | ["[Capturing Images]\nImages were collected using a Keyence BZ-X810 series All-in-one Fluorescence microscope. With BZ-X800 viewer software, each stained slide was imaged on the Brightfield/Phase contrast channel using a 20X objective. The stained region of interest was selected by specifying the XY positions of the t... |
39,713 | Protocol: Phylogenetics analysis of TP53 gene in humans and its use in biosensors for breast cancer diagnosis | 5 | dx.doi.org/10.17504/protocols.io.biz9kf96 | https://www.protocols.io/view/protocol-phylogenetics-analysis-of-tp53-gene-in-hu-biz9kf96 | Sara Da Silva Nascimento, Pierre Teodosio | TITLE: Protocol: Phylogenetics analysis of TP53 gene in humans and its use in biosensors for breast cancer diagnosis
AUTHORS: Sara Da Silva Nascimento, Pierre Teodosio
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Biosensors are small devices that use biological reactions to detect target analytes... | ["[METHODOLOGY]\nProtocol: Phylogenetic analysis of TP53 gene in humans and its use in biosensors for breast cancer diagnosis", "[METHODOLOGY]\nDataset: Initially, 301 sequences of a fragment of the human TP53 gene recovered from GENBANK (https://www.ncbi.nlm.nih.gov/popset/430765060) and participated in a PopSet made ... |
null | null | null | dx.doi.org/10.17504/protocols.io.fxkbpkw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
63,073 | Electrophysiology from cervical vagus nerve and great auricular nerve in swine | 1 | dx.doi.org/10.17504/protocols.io.j8nlkk6n5l5r/v1 | https://www.protocols.io/view/electrophysiology-from-cervical-vagus-nerve-and-gr-b9t9r6r6 | Nishant Verma, Kip Ludwig | TITLE: Electrophysiology from cervical vagus nerve and great auricular nerve in swine
AUTHORS: Nishant Verma, Kip Ludwig
[DESCRIPTION]
This protocol was used to collect data in preparation for publication (Characterization of Electrodes to Record Neural Signals in the Periphery). Additionally, the dataset is public... | ["[Institutional Animal Care and Use Committee Approval] Seek approval from your local IACUC.", "[Anesthesia] Deliver intramuscular injection of Telazol (6 mg/kg) and Xylazine (2 mg/kg) to induce sedation.", "[Cervical vagus nerve preparation] With the subject in a supine position, use a midline approach to access the ... |
43,778 | Semi-automated quantitation of macroautophagy with the auto-QC counter | 4 | dx.doi.org/10.17504/protocols.io.bnzamf2e | https://www.protocols.io/view/semi-automated-quantitation-of-macroautophagy-with-bnzamf2e | Francois Singh, Graeme Ball, Ian Ganley | TITLE: Semi-automated quantitation of macroautophagy with the auto-QC counter
AUTHORS: Francois Singh, Graeme Ball, Ian Ganley
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><span>General macroautophagy is a naturally occurring phenomenon. Based o... | ["[Find the appropriate settings]\nThis section explains how to adjust the settings on a single picture to detect autophagosomes and autolysosomes", "[Find the appropriate settings]\nOpen a picture with high autophagy (e.g. cells stimulated with EBSS):Circle cells as described in", "[Find the appropriate settings]\nSta... |
68,871 | Overall protocol for MicroPOTS LCMS top down proteomics of kidney tissue sections | 1 | dx.doi.org/10.17504/protocols.io.eq2lynm1qvx9/v1 | https://www.protocols.io/view/overall-protocol-for-micropots-lcms-top-down-prote-cfhftj3n | Mowei Zhou, James M Fulcher, Yen-Chen Liao, Ljiljana.PasaTolic | TITLE: Overall protocol for MicroPOTS LCMS top down proteomics of kidney tissue sections
AUTHORS: Mowei Zhou, James M Fulcher, Yen-Chen Liao, Ljiljana.PasaTolic
[DESCRIPTION]
This is the overall workflow for LCMS top down proteomics of kidney functional units from tissue sections using the MicroPOTS platform. The expe... | ["[Tissue collection] The tissue sections were prepared and shipped from Vanderbilt-TMC following the protocol below:", "[Sample preparation] Functional units (glomerulus, medullary, tubule) were dissected and collected into the microPOTS platform using the method below:", "[Data Acquisition] The samples were analyzed ... |
97,873 | Hairy root generation in common bean (Phaseolus vulgaris L.) and selection of Agrobacterium rhizogenes clones | 4 | dx.doi.org/10.17504/protocols.io.261ge3bpjl47/v3 | https://www.protocols.io/view/hairy-root-generation-in-common-bean-phaseolus-vul-dbtr2nm6 | Ronal Pacheco, Georgina Estrada-Navarrete, Noreide Nava, Jorge Solis-Miranda, Carmen Quinto | TITLE: Hairy root generation in common bean (Phaseolus vulgaris L.) and selection of Agrobacterium rhizogenes clones
AUTHORS: Ronal Pacheco, Georgina Estrada-Navarrete, Noreide Nava, Jorge Solis-Miranda, Carmen Quinto
[DESCRIPTION]
The common bean (Phaseolus vulgaris L.) is one of the legumes used to study the molecul... | ["[Seeds disinfection (when necessary)] Immerse the common bean seeds in 96 % volume ethanol for 5 min and wash them three times with sterile water.", "[Seeds disinfection (when necessary)] Immerse the seeds in 2 % volume sodium hypochlorite for 5 min and wash them three times with sterile water.", "[Seeds disinfecti... |
47,805 | Chlamydomonas reinhardtii cell motility quantification | 4 | null | https://www.protocols.io/view/chlamydomonas-reinhardtii-cell-motility-quantifica-bsw5nfg6 | Joao Vitor Molino | TITLE: Chlamydomonas reinhardtii cell motility quantification
AUTHORS: Joao Vitor Molino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocols describe the steps required for the motility quantification in </span><span style = "font-style:italic;">Chlamydomonas reinhardtii</span><span... | ["[Cell preparation]\nCulture the cells for 5 days following the growing Chlamydomonas reinhardtii protocol.Prepare a sample of the cells by washing them in the final desired media by centrifugation. , removal of old media and addition of new media.\nCentrifuge: 3000 34, 3 min, 25 10", "[Glass slide preparation]\nIn g... |
39,035 | Using sequins in metagenome experiments. | 1 | null | https://www.protocols.io/view/using-sequins-in-metagenome-experiments-bic3kayn | Tim Mercer | TITLE: Using sequins in metagenome experiments.
AUTHORS: Tim Mercer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Metagenome sequins are a set of synthetic DNA controls that reflect the sequence complexity, GC content, phylogenetic diversity and abundance of a natural microbial community. The sequ... | ["[Laboratory Steps]\nReceiving sequins.Your sequins should arrive in tubes within a sealed package. Once received, store the sequin tubes at -20C until you are ready to use them.", "[Laboratory Steps]\nPreparing sequin stocks.Meta vector sequins are provided in solution in nuclease-free water at a concentration of 15 ... |
null | null | null | dx.doi.org/10.17504/protocols.io.e87bhzn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
96,215 | PILOT, OPEN NON-CONTROLLED TRIAL TO ASSESS THE FEASIBILITY OF IMPLEMENTING OBJECTIVE PARAMETERS AS PRIMARY ENDPOINTS IN A CLINICAL TRIAL WITH PATIENTS AFFECTED BY KNEE OSTEOARTHRITIS. | 0 | dx.doi.org/10.17504/protocols.io.n2bvj35o5lk5/v1 | https://www.protocols.io/view/pilot-open-non-controlled-trial-to-assess-the-feas-c97xz9pn | Bogdan-Corneliu Andor1, Dionisio Franco Barattini2, Simone Guadagna3, Luca Barattini4, Serban Rosu1, Dumitru-Emanuel Dogaru2 | TITLE: PILOT, OPEN NON-CONTROLLED TRIAL TO ASSESS THE FEASIBILITY OF IMPLEMENTING OBJECTIVE PARAMETERS AS PRIMARY ENDPOINTS IN A CLINICAL TRIAL WITH PATIENTS AFFECTED BY KNEE OSTEOARTHRITIS.
AUTHORS: Bogdan-Corneliu Andor1, Dionisio Franco Barattini2, Simone Guadagna3, Luca Barattini4, Serban Rosu1, Dumitru-Emanuel Dog... | [] |
101,766 | Evaluation of the Antibacterial efficacy of Allium Sativum Gel as Intracanal Medicament Versus Calcium Hydroxide Paste Against Enterococcas faccalis in Single Rooted Teeth (A Comparative In-Vitro Study) | 0 | dx.doi.org/10.17504/protocols.io.36wgqndp3gk5/v1 | https://www.protocols.io/view/evaluation-of-the-antibacterial-efficacy-of-allium-dfme3k3e | Rania Ahmed Ahmed ELnaa | TITLE: Evaluation of the Antibacterial efficacy of Allium Sativum Gel as Intracanal Medicament Versus Calcium Hydroxide Paste Against Enterococcas faccalis in Single Rooted Teeth (A Comparative In-Vitro Study)
AUTHORS: Rania Ahmed Ahmed ELnaa
[DESCRIPTION]
Objectives
The aim of this study is to evaluate the antimicrob... | [] |
54,340 | Amplification of DNA | 1 | dx.doi.org/10.17504/protocols.io.bzbcp2iw | https://www.protocols.io/view/amplification-of-dna-bzbcp2iw | Yuichiroh Ikagawa | TITLE: Amplification of DNA
AUTHORS: Yuichiroh Ikagawa
[DESCRIPTION]
Our project aims to quantify human fatigue through the quantification of the biomarker human herpesvirus6 using Cas12a, a protein that is fundamental and essential to our project. We ordered the synthesis of composite parts with affinity tags att... | ["Thaw the DNA solution at room temperature, and KAPA HiFi HotStart ReadyMix (x2) on ice.", "Vortex the reagents, then centrifuge them briefly in a microcentrifuge.", "Mix the reagents according to the composition in the table below.", "For each template DNA, select the temperature setting of the thermal cycler listed ... |
29,736 | Swabbing scats for DNA sampling - field protocol | null | dx.doi.org/10.17504/protocols.io.9agh2bw | null | Anna MacDonald | TITLE: Swabbing scats for DNA sampling - field protocol
AUTHORS: Anna MacDonald
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to swab mammal scats in the field, for DNA sampling. Swabs are air-dryed for storage in the field and transport to the laboratory.</div></div>
... | ["Before touching the scat:photograph the scatassign a sample numberrecord GPS coordinatesrecord any other required field data", "Swab the scat before otherwise moving or disturbing it.\nWear clean gloves for every sample. Change gloves as often as needed to avoid contamination.", "Select a clean, unused swab and check... |
15,458 | High-Throughput Echo Library Prep Protocol | 1 | dx.doi.org/10.17504/protocols.io.tcaeise | https://www.protocols.io/view/high-throughput-echo-library-prep-protocol-tcaeise | Madeline Mayday, Lillian Khan, Eric D. Chow, Matt S. Zinter, Joseph L. DeRisi | TITLE: High-Throughput Echo Library Prep Protocol
AUTHORS: Madeline Mayday, Lillian Khan, Eric D. Chow, Matt S. Zinter, Joseph L. DeRisi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = ":justify;">Preparation of high-quality sequencing libraries is a costly and time-consuming component... | ["Spin plate of sample RNA in vacuum evaporator at the appropriate temperature and time settings to dry completely (approximately 40ºC for 25-30 minutes, depending on number of samples and machine used).", "[PREPARE INITIAL RNA SAMPLE PLATE]\nLoad 5uL of sample RNA into a 384-well PCR plate. Repeat with all desired sam... |
99,797 | Fixation and Immunostaining protocol | 0 | dx.doi.org/10.17504/protocols.io.j8nlk8wowl5r/v1 | https://www.protocols.io/view/fixation-and-immunostaining-protocol-ddpv25n6 | gustavo.parfitt Parfitt, Gist Croft | TITLE: Fixation and Immunostaining protocol
AUTHORS: gustavo.parfitt Parfitt, Gist Croft
[DESCRIPTION]
Fixation and Immunostaining protocol for tissue and cell culture
[STEPS]
1. Prepare 1x Fix by dilution from 2x Fix (see Description)
2. Gently aspirate culture supernatant, wash gently with PBS, and replace with col... | ["Prepare 1x Fix by dilution from 2x Fix (see Description)", "Gently aspirate culture supernatant, wash gently with PBS, and replace with cold Fix buffer", "Incubate 30min @ 4° C or on ice", "Aspirate fix and wash gently 3x with PBS (incubate 1-2min each)", "Aspirate and add Quench to permeabilize cells/tissue, at lea... |
28,768 | Nuclei-seq | null | dx.doi.org/10.17504/protocols.io.8b8hsrw | null | Mandy Xu, Troy Ketela | TITLE: Nuclei-seq
AUTHORS: Mandy Xu, Troy Ketela
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol has been developed by the Princess Margaret Genomics Centre specifically for Nuc-Seq, both at the bulk and single-cell levels. </div></div>
[STEPS]
?. [Nuclei-seq: Preparation]
Rack 4 falc... | ["[Nuclei-seq: Preparation]\nRack 4 falcon tubes (lysis buff, wash buff, nuclei, intermediate) and 1 FACS tube on ice", "[Nuclei-seq: Preparation]\nChill centrifuges (4°C 800 g 10 min), clean hood, fill Styrofoam boxes: one with dry ice (petri dish and razor blade); one with ice packed and slanted for petri dish away f... |
null | null | null | dx.doi.org/10.17504/protocols.io.fuwbnxe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol will introduce a workflow for quality control and pre-processing of metagenomic sequence reads using FastQC for visualization and FastX Toolkit for editing the fastq files. </p>
[GUIDELINES]
<p><a href="http://hannonlab.cshl.edu/fastx_toolkit/index.html" target... | [] |
98,275 | Embryonic/Postnatal Mouse Neuron Culture Protocol | 0 | dx.doi.org/10.17504/protocols.io.14egn68jql5d/v1 | https://www.protocols.io/view/embryonic-postnatal-mouse-neuron-culture-protocol-db8b2rsn | Michael X. Henderson | TITLE: Embryonic/Postnatal Mouse Neuron Culture Protocol
AUTHORS: Michael X. Henderson
[DESCRIPTION]
This protocol details the embryonic/postnatal neuron culture procedures.
[GUIDELINES]
*Simplified protocol for neuron culture (hippocampal or cortical)
All procedures on live animals should be performed in accordance ... | ["[Coverslip and plate preparation] Drop 12 mm coverslips into a 200 ml beaker containing 95% Ethanol (or methanol). (Leave at least 1 min).", "[Coverslip and plate preparation] Plate coverslips on a 24-well plate and allow to completely dry (>30 min).", "[Coverslip and plate preparation] Dilute PDL to 100 1527 with bo... |
8,184 | Synthesis of derivatization reagent TAHS (N,N,N-trimethylamonioanilyl N-hydroxysuccinimidyl carbamate iodide) | null | dx.doi.org/10.17504/protocols.io.j8ycrxw | null | Manuel Liebeke | TITLE: Synthesis of derivatization reagent TAHS (N,N,N-trimethylamonioanilyl N-hydroxysuccinimidyl carbamate iodide)
AUTHORS: Manuel Liebeke
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">TAHS was synthetized according to the protocol previously described with minor modifications. TAHS is used as d... | ["6.0 g (23.4 mmol) DSC was dissolved in 250 mL of dry ACN to which 3.0 g (22.0 mmol) DPD in 250 mL dry ACN was added dropwise over a period of 45 min and stirred (Room temperature)DSC Molecular Weight 256.17 C9H8N2O7DPD Molecular Weight 136.19 (CH3)2NC6H4NH2\n[DSC]\n[DPD]\n[ACN]", "The product was concentrated using r... |
38,466 | Quantification of tube topography on micrographs of fluorescent staining of multi-layers cell culture. | 5 | dx.doi.org/10.17504/protocols.io.bhtaj6ie | https://www.protocols.io/view/quantification-of-tube-topography-on-micrographs-o-bhtaj6ie | Bertrand-David Segard | TITLE: Quantification of tube topography on micrographs of fluorescent staining of multi-layers cell culture.
AUTHORS: Bertrand-David Segard
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">The induction or inhibition of angiogenesis can be assessed in... | ["[Software]\nInstall Icy (requires JDK 8+).", "[Software]\nDownload the Icy script \"ImageJ background subtraction.\" (file name: protocolfile-imagej-background-subtraction1)See: http://icy.bioimageanalysis.org/protocol/imagej-background-subtraction/", "[Software]\nInstall the Icy plugin \"Membrane Filter.\"See: http:... |
29,671 | RNA extraction from 10µL mouse blood samples (KingFisher Flex 96-well) | null | dx.doi.org/10.17504/protocols.io.88fhztn | null | Petra Schneider, Charlotte Repton, Sarah Reece | TITLE: RNA extraction from 10µL mouse blood samples (KingFisher Flex 96-well)
AUTHORS: Petra Schneider, Charlotte Repton, Sarah Reece
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>RNA extraction from 5uL mouse blood samples, for subsequent cDNA production and qPCR to detect </span><span styl... | ["[Sample Collection]\nDispense 20 µL of RNAlater into 0.2 mL strip tubes or 96-well plates, one well per sample. Cover tubes with lids, or plate with a plastic film (the thick version, not the optical film for qPCRs – that one will NOT come off). Note: using more RNAlater ends up inhibiting the downstream process.", ... |
78,444 | Acclimation of in vitro grown individual lines of big sagebrush (Artemisia tridentata) to ex vitro conditions in support of genotype-by-environment experiments and restoration | 4 | dx.doi.org/10.17504/protocols.io.j8nlk4zpxg5r/v3 | https://www.protocols.io/view/acclimation-of-in-vitro-grown-individual-lines-of-cqukvwuw | Peggy Martinez, Marcelo Serpe, Rachael Barron, Sven Buerki | TITLE: Acclimation of in vitro grown individual lines of big sagebrush (Artemisia tridentata) to ex vitro conditions in support of genotype-by-environment experiments and restoration
AUTHORS: Peggy Martinez, Marcelo Serpe, Rachael Barron, Sven Buerki
[DESCRIPTION]
The following protocol describes the ex vitro,... | ["[Prepare sandy soil with nutrient solution] Prepare sandy soil\n\nMix play sand and vermiculite to a 4:1 v/v (sand/vermiculite) in large plastic tub (hereafter referred to as sandy soil). If play sand is very dry, add enough DI water to moisten the sandy soil mixture.\n \n\n2. Tare PhytoCon vessel (946mL) on the bala... |
90,993 | Working with patient-derived enteroids and colonoids | 4 | dx.doi.org/10.17504/protocols.io.ewov1q7e2gr2/v1 | https://www.protocols.io/view/working-with-patient-derived-enteroids-and-colonoi-c44ryyv6 | Tatiana Karakasheva | TITLE: Working with patient-derived enteroids and colonoids
AUTHORS: Tatiana Karakasheva
[DESCRIPTION]
This is a compilations of working with patient-derived enteroids or colonoids, from recovery to cryopreservation, in expansion culture (minimal differentiation)
[STEPS] | [] |
62,989 | a-Synuclein protein expression and purification | 4 | dx.doi.org/10.17504/protocols.io.3byl4brwrvo5/v1 | https://www.protocols.io/view/a-synuclein-protein-expression-and-purification-b9rmr546 | andrew.west, arpine.sokratian | TITLE: a-Synuclein protein expression and purification
AUTHORS: andrew.west, arpine.sokratian
[DESCRIPTION]
Protocol for recombinant a-synuclein purification, useful as monomer template for seeded-amplification assays (like RT_QUIC or PMCA) . Recommendations are to store the protein always on ice while not running and... | ["[Transformation] Thaw down an aliquot of plasmid construct (pRK172) encoding WT-human-a-synuclein 0.3 mg/mL on ice", "[Transformation] Thaw down on ice an aliquot of BL21 (DE3) RIL competent E Coli cells", "[Transformation] Add 1 µL of plasmid construct to the thawed competent cells and gently mix by flicking the b... |
null | null | null | dx.doi.org/10.17504/protocols.io.phbdj2n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><u>Goal:</u></p>
<p>This document aims to standardize the protocol for the staining of lipid droplets with Oil Red O (ORO), and subsequent observation using fluorescence microscopy.</p>
<p>The technique of staining with Oil Red O can be used to observe changes in the lipid me... | [] |
72,039 | Analysis of glycosphingolipids from animal tissues | 1 | dx.doi.org/10.17504/protocols.io.n2bvj88rbgk5/v1 | https://www.protocols.io/view/analysis-of-glycosphingolipids-from-animal-tissues-cikfuctn | David A Priestman, Marya Sabir, Reuben Bush, Danielle te Vruchte, Kerri-Lee Wallom, María E Fernández-Suárez, Frances M Platt | TITLE: Analysis of glycosphingolipids from animal tissues
AUTHORS: David A Priestman, Marya Sabir, Reuben Bush, Danielle te Vruchte, Kerri-Lee Wallom, María E Fernández-Suárez, Frances M Platt
[DESCRIPTION]
Interest in the role of cellular glycosphingolipids (GSLs) in health and disease led to us developing a sensitiv... | ["[GSL preparation from animal tissues] Add 0.8 mL of chloroform/methanol (1:2, v/v) to give (C/M/W 4:8:3 final).", "[GSL preparation from animal tissues] Leave at 4 °C.", "[GSL preparation from animal tissues] Vortex.", "[GSL preparation from animal tissues] Centrifuge at 16,000 x g for 10 min at 4 Room temperature."... |
88,590 | Labeling of host immune cells with magnetic nanoparticles | 4 | dx.doi.org/10.17504/protocols.io.36wgq36z3lk5/v1 | https://www.protocols.io/view/labeling-of-host-immune-cells-with-magnetic-nanopa-c2rnyd5e | Eric J. Koch, Jamie S Foster | TITLE: Labeling of host immune cells with magnetic nanoparticles
AUTHORS: Eric J. Koch, Jamie S Foster
[DESCRIPTION]
This protocol describes a technique to label the innate immune cells of a host squid, known as hemocytes, with commercially available magnetic nanoparticles (i.e., magdots). The goal of this technique i... | ["[Preparation of target immune cells - squid hemocytes] Collect sample of target immune cells. Here, hemocytes extracted from the bobtail squid Euprymna scolopes were used.", "[Preparation of target immune cells - squid hemocytes] Using an insulin 1 cc syringe (30 gauge needle) collect hemolymph from the cephalic arte... |
106,863 | procedure20240903 | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1no2lmk/v1 | https://www.protocols.io/view/procedure20240903-dkkp4uvn | Yasuhiro Ooshima | TITLE: procedure20240903
AUTHORS: Yasuhiro Ooshima
[DESCRIPTION]
This protocol is used in 『Impact of perspective-taking training under relational frame theory for the organizational
model of empathy』
[STEPS]
SECTION: Entire procedure
1.
The entire experiment was conducted online
using Zoom, a videoconferencing... | ["[Entire procedure] The entire experiment was conducted online\nusing Zoom, a videoconferencing platform (Fig 1). Upon joining Zoom,\nparticipants \n \nwere initially apprised of the study’s emphasis on understanding\nperspectives different from their own and were informed of the training they\nwould receive. Subseque... |
86,201 | Inducing proteostasis stress using G-TPP | 1 | dx.doi.org/10.17504/protocols.io.j8nlkowr1v5r/v1 | https://www.protocols.io/view/inducing-proteostasis-stress-using-g-tpp-cyezxtf6 | Louise Uoselis | TITLE: Inducing proteostasis stress using G-TPP
AUTHORS: Louise Uoselis
[DESCRIPTION]
Inducing proteostasis stress in HeLa cells with G-TPP to analyse proteostasis protection and repair.
[STEPS]
SECTION: Day 1
1. Seed cells in standard growth media, aiming for a density of ~80-90% the following day.
SECTION: Day 3
4... | ["[Day 1] Seed cells in standard growth media, aiming for a density of ~80-90% the following day.", "[Day 3] Harvest the G-TPP treated samples and DMSO control samples after the desired incubation period (eg. 2 – 12 h).", "[Day 2] Feed cells for 60 min prior to starting treatment by aspirating the media from each well ... |
32,468 | Generation of hCS from hiPSC maintained in feeder-free conditions | null | null | https://www.protocols.io/view/generation-of-hcs-from-hipsc-maintained-in-feeder-bbxuipnw | Sejin Yoon, Sergiu P. Pașca | TITLE: Generation of hCS from hiPSC maintained in feeder-free conditions
AUTHORS: Sejin Yoon, Sergiu P. Pașca
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Here, we show the generation of 3D human cortical spheroids (hCS) from human induced pluripotent stem cells (hiPSC) maintained in </span... | ["[Maintenance and long-term culture]\nIncubate intact spheroids with Fluo-4 diluted in NM for at and 5% CO2, followed by a wash.\n37 °C", "[Maintenance and long-term culture]\nUsing an appropriate imaging system, record spontaneous calcium activity for several minutes (one frame every 8–10 s) in one 10-μm z-stack ... |
40,845 | Streaking and Isolating Bacteria on an LB Agar Plate - CHEM 584 | 1 | dx.doi.org/10.17504/protocols.io.bj5mkq46 | https://www.protocols.io/view/streaking-and-isolating-bacteria-on-an-lb-agar-pla-bj5mkq46 | Addgene The Nonprofit Plasmid Repository, Ken Christensen | TITLE: Streaking and Isolating Bacteria on an LB Agar Plate - CHEM 584
AUTHORS: Addgene The Nonprofit Plasmid Repository, Ken Christensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes how to streak and isolate (single colony) bacteria on an LB agar plate. To see the f... | ["[Day 1]\nObtain an LB agar plate with appropriate antibiotic.", "[Day 1]\nLabel the bottom of the plate with the plasmid name and the date. It is also a good idea to add the antibiotic resistance and your initials. Labeling within a laboratory setting is important for organization, and it is recommended that you keep... |
50,501 | Expression and purification protocol of GST-NDP52 | 1 | dx.doi.org/10.17504/protocols.io.bvjdn4i6 | https://www.protocols.io/view/expression-and-purification-protocol-of-gst-ndp52-bvjdn4i6 | Chunmei Chang | TITLE: Expression and purification protocol of GST-NDP52
AUTHORS: Chunmei Chang
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the expression and purification of GST-NDP52.</div></div>
[STEPS]
?. [Protein expression]
Transform the E.Coli BL21DE3 cells with plasmid encoding f... | ["[Protein expression]\nTransform the E.Coli BL21DE3 cells with plasmid encoding for GST-NDP52 and plate them on Amp plate.", "[Protein expression]\nCarry out protein expression in medium, induce with IPTG (isopropyl- -d-thiogalactopyranoside) to an OD600 of 0.8 and grow at .\n1.5 L\n18 °C", "[Protein expression]\nH... |
97,103 | USDA LTAR Common Experiment measurement: Butterfly diversity and abundance | 1 | dx.doi.org/10.17504/protocols.io.14egn6bnpl5d/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-butterfly-da3p2gmn | Nick M. Haddad, Jamie D. Smith | TITLE: USDA LTAR Common Experiment measurement: Butterfly diversity and abundance
AUTHORS: Nick M. Haddad, Jamie D. Smith
[DESCRIPTION]
We will use a measure of butterfly abundance and diversity collected using a method standard across the US and the globe (Wepprich, et al. 2019). This method is used in regional studi... | ["[Pollard walks] Transect routes are established as appropriate for each site.", "[Pollard walks] The recommended transect length is 1 km and the walking duration is 1 hour and 30 minutes.", "[Pollard walks] Transects are marked before surveys commence, and the same transect is sampled throughout the research effort."... |
46,993 | Fish eDNA: water sampling and filtration through Sterivex filter unit | 4 | dx.doi.org/10.17504/protocols.io.br5rm856 | https://www.protocols.io/view/fish-edna-water-sampling-and-filtration-through-st-br5rm856 | Marine Vautier, Cécile Chardon, Isabelle Domaizon | TITLE: Fish eDNA: water sampling and filtration through Sterivex filter unit
AUTHORS: Marine Vautier, Cécile Chardon, Isabelle Domaizon
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The objective of this protocol is the sampling and the filtration of water samples through 0.45 µm Sterivex™ filt... | ["[WATER SAMPLING]\nPut on gloves and open the bottle without touching the inside of the cap or the neck of the bottle.Collect by hand subsurface water (10-20 cm below the water surface) and close the bottle carefully. If water is collected deeper with a sampling tank, it must also be decontaminated prior to sampling.... |
106,180 | Immunofluorescence protocol for FFPE human post-mortem brain sections to detect alpha-synuclein and tau pathology | 0 | dx.doi.org/10.17504/protocols.io.n92ld8nxxv5b/v2 | https://www.protocols.io/view/immunofluorescence-protocol-for-ffpe-human-post-mo-djxc4piw | Felicia Suteja, Hongyun Li, YuHong Fu | TITLE: Immunofluorescence protocol for FFPE human post-mortem brain sections to detect alpha-synuclein and tau pathology
AUTHORS: Felicia Suteja, Hongyun Li, YuHong Fu
[DESCRIPTION]
This protocol details the method for preparing and staining human formalin-fixed, paraffin embedded post mortem brain tissue to detect al... | ["[Day I - Tissue Prep] Place slides with FFPE human brain tissues on a slide rack and bake in a 60 °C oven for 60 min", "[De-paraffinize] 2. Continue using the slide rack, submerge slides in the following solution to de-paraffinize: \n a. HistoChoice: 2 x 7 min\n b. 100% ethanol: 2 x 3 min \n c. 95% eth... |
53,705 | Lentivirus production & concentration | 4 | null | https://www.protocols.io/view/lentivirus-production-amp-concentration-byphpvj6 | Allan JW Lui | TITLE: Lentivirus production & concentration
AUTHORS: Allan JW Lui
[DESCRIPTION]
Lentivirus production protocol based on official protocol for Lipofectamine 3000 and TransIT-Lenti
[BEFORE_START]
Prepare culture media for HEK293T:
supplemented with 10%
[STEPS]
SECTION: HEK293T seeding density titration
1. D... | ["[HEK293T seeding density titration] Detach, count & seed HEK293T cells into T75 flasks at:\n4 x 10E6 cells\n6 x 10E6 cells\n8 x 10E6 cells\n10 x 10E6 cells", "[HEK293T seeding density titration] Incubate plates at 37 °C for up to 1440 min \nObserve confluence of cells under a microscope at 18 - 24 hours after plating... |
66,369 | GrownMD CBD Gummies (Pros and Cons) Is It Scam Or Trusted? | 1 | dx.doi.org/10.17504/protocols.io.ewov1nwo2gr2/v1 | https://www.protocols.io/view/grownmd-cbd-gummies-pros-and-cons-is-it-scam-or-tr-cc29syh6 | hafaviy | TITLE: GrownMD CBD Gummies (Pros and Cons) Is It Scam Or Trusted?
AUTHORS: hafaviy
[DESCRIPTION]
GrownMD CBD Gummies
[STEPS]
1. What are Grown MD CBD Gummies?
Grown MD CBD Gummiesare a reasonable extraordinary and hearty course of activity. They incorporate present day and standard adornments to address or fix log... | ["What are Grown MD CBD Gummies?\n\nGrown MD CBD Gummiesare a reasonable extraordinary and hearty course of activity. They incorporate present day and standard adornments to address or fix logical issues.\n\nYou need to kill the cannabinoids from the plant with exorbitant mindfulness. It very well might be joined with ... |
63,010 | Real time-quaking induced conversion assay (RT-QUIC) | 4 | dx.doi.org/10.17504/protocols.io.6qpvr67kpvmk/v1 | https://www.protocols.io/view/real-time-quaking-induced-conversion-assay-rt-quic-b9sar6ae | andrew.west, arpine.sokratian | TITLE: Real time-quaking induced conversion assay (RT-QUIC)
AUTHORS: andrew.west, arpine.sokratian
[DESCRIPTION]
Seeded-amplification assay (SAA) method for the detection of pathology-associated aggregation-templating competent forms of alpha-synuclein and other interacting factors.
[STEPS]
SECTION: Plate preparation... | ["[Plate preparation] Place single zirconia bead into each well of the", "[Plate preparation] Thaw down a-syn monomer and sonicated fibril aliquots on ice, do not generate bubbles by pipetting", "[Plate preparation] Measure monomer concentration via Nanodrop\nAdd 3 µL of 10x diluted aliquot in PBS onto nanodrop piedest... |
null | null | null | dx.doi.org/10.17504/protocols.io.mu5c6y6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The aim of the current study was to investigate whether optimism and self-efficacy mediated the association between shyness and subjective well-being in a sample of Chinese working adults. Two hundred and eight participants completed the Revised Cheek and Buss Shyness Scale, ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.p2hdqb6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>this protocol describes the maintainance and treatment of RAW 264.7 macrophage cell lines with Mycobacterial PE/PPE proteins. It also describes estimation of secreted cytokines through ELISA. Estimation of level of IgG subclass from the serum is also described here.</p>
[STE... | [] |
71,933 | SRB assay for measuring target cell killing | 4 | dx.doi.org/10.17504/protocols.io.e6nvwjk7dlmk/v1 | https://www.protocols.io/view/srb-assay-for-measuring-target-cell-killing-cig5uby6 | Diana Rose E Ranoa | TITLE: SRB assay for measuring target cell killing
AUTHORS: Diana Rose E Ranoa
[DESCRIPTION]
The Sulforhodamine B colorimetric assay was used to measure effector CAR T cell killing of their target tumor cells.
[STEPS]
1. Perform co-culture experiment of activated CAR T cells and target tumor cells at various tumor:e... | ["Perform co-culture experiment of activated CAR T cells and target tumor cells at various tumor:effector ratio in a 96-well plate for 24 hours at 37'C, 5% CO2.", "Remove cell culture supernatant 24 hours after co-culture assay.", "Using a multichannel pipet, fix adherent tumor cells on the 96-well plate with 100uL col... |
70,509 | Fast-S: Single tube amplification and PCR barcoding of SARS-CoV-2 S gene for Nanopore sequencing | 1 | null | https://www.protocols.io/view/fast-s-single-tube-amplification-and-pcr-barcoding-cg4mtyu6 | Cecilia Salazar | TITLE: Fast-S: Single tube amplification and PCR barcoding of SARS-CoV-2 S gene for Nanopore sequencing
AUTHORS: Cecilia Salazar
[DESCRIPTION]
Most of the defining mutations of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VOCs) have been identified in the S gene sequence. For ... | ["[Reverse transcription] Keeping the SARS-CoV-2 extracted RNA samples on ice all the time and spin down the tubes.", "[Reverse transcription] Set up the RT-PCR reaction tubes in a clean pre-PCR cabinet by adding 2 μL of LunaScript® RT SuperMix to each PCR tube. Include a RT-PCR negative control by replacing RNA sampl... |
92,513 | A standard pipeline for processing short-read sequencing data from Littorina snails | 1 | null | https://www.protocols.io/view/a-standard-pipeline-for-processing-short-read-sequ-c6j9zcr6 | James Reeve, Amin Ghane, Pierre Barry, Alfonso Balmori-de la Puente, Roger K. Butlin, Le Qin Choo, Alan Le Moan, Diego Garica Castillo, Ana-Maria Peris Tamayo, Sarah Kingston, Erica Leder, Sean Stankowski | TITLE: A standard pipeline for processing short-read sequencing data from Littorina snails
AUTHORS: James Reeve, Amin Ghane, Pierre Barry, Alfonso Balmori-de la Puente, Roger K. Butlin, Le Qin Choo, Alan Le Moan, Diego Garica Castillo, Ana-Maria Peris Tamayo, Sarah Kingston, Erica Leder, Sean Stankowski
[DESCRIPTION]
... | ["[Overview] Processing of short read sequencing data can be broken down into four major steps (Fig. 1): \n 1) read quality inspection and trimming\n 2) read mapping\n 3) variant calling \n 4) variant filtering\nThere are numerous sub-steps in between, which together have a plethora of different adj... |
99,398 | IRIS Software Protocol | 0 | dx.doi.org/10.17504/protocols.io.3byl497wjgo5/v2 | https://www.protocols.io/view/iris-software-protocol-ddbe22je | Leonardo Zilli, Erica Andreose, Salvatore Di Marzo | TITLE: IRIS Software Protocol
AUTHORS: Leonardo Zilli, Erica Andreose, Salvatore Di Marzo
[DESCRIPTION]
This project aims to investigates the discoverability of University of Bologna's scholarly output within OpenCitations Meta, a platform that stores and delivers bibliographic metadata for alle the publications invol... | ["[IRIS Dataset Preparation] After downloading the IRIS dataset, the contents appear divided into 7 different csv files, descriptive as the following:\n- \"ODS_L1_IR_ITEM_CON_PERSON.csv\": information about the people involved in the publications (authors, editors, etc.)\n- \"ODS_L1_IR_ITEM_DESCRIPTION.csv\": the strin... |
5,108 | Electroporation of Agrobacterium | null | dx.doi.org/10.17504/protocols.io.g8ubzww | null | Johannes Wolfram Debler | TITLE: Electroporation of Agrobacterium
AUTHORS: Johannes Wolfram Debler
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>A quick guide on how to electroporate your plasmid of interest into electrocompetent </span><span style = "font-style:italic;">Agrobacterium tumefaciens </span><span>cells.<... | ["[Preparation of Electroporation mixture]\nGet aliquotted electrocompetent Agrobacteria from -80°C freezer\n{\"blocks\":[{\"key\":\"6ojgv\",\"text\":\"This protocol yields about 5 ml of electrocompetent Agrobacterium tumefaciens cells, aliquotted into 80 ul you get 50-60 tubes out of it.\",\"type\":\"unstyled\",\"dept... |
69,788 | Primary Prostate Cell Culture from prostatectomies | 4 | dx.doi.org/10.17504/protocols.io.5qpvornyzv4o/v1 | https://www.protocols.io/view/primary-prostate-cell-culture-from-prostatectomies-cgd4ts8w | Mira Jung | TITLE: Primary Prostate Cell Culture from prostatectomies
AUTHORS: Mira Jung
[DESCRIPTION]
Based on the tissue collection protocol under the Georgetown University’s Institutional Review Board approval, fresh clinical specimens were transferred from the operating room to surgical pathology, processed and examined by th... | ["[Isolation of primary cells from human prostate tissue] Working under a biosafety cabinet, transfer the fresh tissue into the plate.", "[Isolation of primary cells from human prostate tissue] With a sterile blade, make a fine mince of the tissues.", "[Isolation of primary cells from human prostate tissue] Add 5 ml of... |
70,819 | P4 published - redesign - collection | 6 | dx.doi.org/10.17504/protocols.io.4r3l278djg1y/v1 | https://www.protocols.io/view/p4-published-redesign-collection-chebt3an | Maria Gul, katarina | TITLE: P4 published - redesign - collection
AUTHORS: Maria Gul, katarina
[DESCRIPTION]
Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Integer in sapien. Praesent id justo in neque elementum ultrices. Cras elementum. Sed ac dolor sit amet purus malesuada congue. Nunc auctor. Quis autem vel eum iure reprehend... | ["Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Integer in sapien. Praesent id justo in neque elementum ultrices. Cras elementum. Sed ac dolor sit amet purus malesuada congue. Nunc auctor. Quis autem vel eum iure reprehenderit qui in ea voluptate velit esse quam nihil molestiae consequatur, vel illum qui do... |
68,489 | An ImageJ macro for batch processing of microscopic images prior to CellProfiler automated analysis | 5 | dx.doi.org/10.17504/protocols.io.3byl4bpo8vo5/v1 | https://www.protocols.io/view/an-imagej-macro-for-batch-processing-of-microscopi-ce5htg36 | Herschel Dhekne, Suzanne R Pfeffer | TITLE: An ImageJ macro for batch processing of microscopic images prior to CellProfiler automated analysis
AUTHORS: Herschel Dhekne, Suzanne R Pfeffer
[DESCRIPTION]
This protocol presents an ImageJ macro for batch processing of microscopic images prior to CellProfiler automated analysis. For many applications includi... | ["The method involves the following steps:\n\n Step 1 - Define the suffix for the processed files\n Step 2 - Define the plugins and parameters that need to be run\n Step 3 - Identify the folder that contains the .TIF raw files\n Step 4 - Select the channel that needs to processed\n Step 5 - Identify the folder where th... |
null | null | null | dx.doi.org/10.17504/protocols.io.qjydupw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa ... | [] |
30,887 | CODEX Microtomy Tracking Sheet | null | dx.doi.org/10.17504/protocols.io.baefibbn | null | Leigh Propper, Marda Jorgensen | TITLE: CODEX Microtomy Tracking Sheet
AUTHORS: Leigh Propper, Marda Jorgensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Microtomy Tracking Sheet for CODEX Specimens</div><div class = "text-block">The process of sectioning CODEX specimens presents tediously at the tissue block trimming and micr... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fjgbkjw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
99,911 | Brain Dissection of Post-natal Mice | 1 | dx.doi.org/10.17504/protocols.io.j8nlken35l5r/v4 | https://www.protocols.io/view/brain-dissection-of-post-natal-mice-ddtf26jn | Allen Institute for Brain Science | TITLE: Brain Dissection of Post-natal Mice
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
This protocol describes the procedure for removal and embedding of the post-natal mouse brain.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Instit... | [] |
46,101 | My new protocol (fork 1) | 1 | dx.doi.org/10.17504/protocols.io.bq9vmz66 | https://www.protocols.io/view/my-new-protocol-fork-1-bq9vmz66 | Monica Hassan | TITLE: My new protocol (fork 1)
AUTHORS: Monica Hassan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">any</div></div>
[STEPS]
?. Step 2 | ["Step 2"] |
98,036 | Test protocol - for collection 2 | 0 | dx.doi.org/10.17504/protocols.io.yxmvmexn6g3p/v1 | https://www.protocols.io/view/test-protocol-for-collection-2-dbyu2pww | Gabriel Gasque | TITLE: Test protocol - for collection 2
AUTHORS: Gabriel Gasque
[DESCRIPTION]
Abstract for first protocl
[STEPS]
1. Step 1
2. Step 2
3. Step 3
4. Step 4 | ["Step 1", "Step 2", "Step 3", "Step 4"] |
null | null | null | dx.doi.org/10.17504/protocols.io.qxwdxpe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol for making invertedClampFISH probes. </p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
19,749 | U Michigan - Cryoembedding | null | dx.doi.org/10.17504/protocols.io.xidfka6 | null | Malcolm Low | TITLE: U Michigan - Cryoembedding
AUTHORS: Malcolm Low
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. Procedure: Fixed Tissue 1. Following fixation (immersion or perfusion), rinse tissue in phosphate buffer (PB, 0.1M, pH 7.3) containing 5, 10, and 20% sucrose for 24 hours per step or until the tissue "s... | ["Procedure: Fixed Tissue 1. Following fixation (immersion or perfusion), rinse tissue in phosphate buffer (PB, 0.1M, pH 7.3) containing 5, 10, and 20% sucrose for 24 hours per step or until the tissue \"sinks” to the bottom of the container or tube. The final sucrose concentration in the tissue may vary from 5-30% d... |
39,618 | Fixing Cell Pellets for Flow Cytometry | 1 | dx.doi.org/10.17504/protocols.io.bixakfie | https://www.protocols.io/view/fixing-cell-pellets-for-flow-cytometry-bixakfie | Verity Goodwin, Emily Souster, Mathew Garnett, Fiona Behan, Charlotte Beaver, Rizwan Ansari, Adam Jackson | TITLE: Fixing Cell Pellets for Flow Cytometry
AUTHORS: Verity Goodwin, Emily Souster, Mathew Garnett, Fiona Behan, Charlotte Beaver, Rizwan Ansari, Adam Jackson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is designed to outline the process of fixing cell pellets in 1.5ml tubes. It ... | ["[Fixing]\nIn a fume hood, prepare a solution of 3.7% formaldehyde in PBS\nChemical safety: Formaldehyde 37% and 3.7% must be prepared and used only in the chemical fume hood, using chemical resistant gloves. Waste must be kept in the fume hood and disposed of appropriately.", "[Fixing]\nIn the fume hood, tap the tube... |
null | null | null | dx.doi.org/10.17504/protocols.io.tcxeixn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>We aimed to compare the effectiveness of supraglottic airway devices as a conduit for unassisted tracheal intubation. We searched OVID-MEDLINE, EMBASE, the Cochrane Central Register of Controlled Trials, KoreaMed, and Google Scholar databases to identify all relevant randomiz... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hivb4e6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes the Immunostaining in fly NMJ boutons. It is from 'Flower Ca<sup>2+</sup> channel in CME and ADBE' of Yao CK et al.</p>
<p> </p>
<p>Please see the manuscript for the full method details.</p>
[GUIDELINES]
<p style="text-align: justify;">Primary antibod... | [] |
20,714 | UC Davis - Corticosterone RIA | null | dx.doi.org/10.17504/protocols.io.ygiftue | null | Peter Havel | TITLE: UC Davis - Corticosterone RIA
AUTHORS: Peter Havel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block"><span>The RIA (RadioImmuno Assay) is an assay method used for the quantification of various proteins. A standard... | ["Dilute samples 1:200 with steroid diluent.", "Add 0.3 ml diluent to NSB tubes and 0.1 ml to zero tubes.", "Add 0.1 ml of calibrators and diluted samples.", "Add 0.1 ml of tracer to all tubes.", "Add 0.1 ml of antibody to all tubes except Total Count and NSB tubes.", "Incubate at RT for 2 hrs.", "Add precipitating rea... |
null | null | null | dx.doi.org/10.17504/protocols.io.q7edzje | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div title="Page 1">
<p>Protocol for thymus dissociation (10-week old CD-1 female). </p>
</div>
[GUIDELINES]
<div title="Page 1">
<div>
<div>
<p><strong>Storage Conditions of Reagents </strong></p>
<p> </p>
<table>
<tbody>
<tr>
<td>
<div title="Page 1">
<div>
<div>
<div>
<p><st... | [] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.