text stringlengths 0 4.18k |
|---|
Pipette tip A shows wetting failure. Pipette tips B-C show |
uniform emulsions & slightly low volumes. (~95 μ l) Most wetting failures will not impact emulsion volumes of other sample run on the chip. |
Occasionally, wetting failures may impact the emulsion volumes recovered from other lanes. This is expected and does not indicate an emulsion failure if the samples are uniform in volume and opacity (B-C). |
<!-- image --> |
In pipette tips A-E & G-H, a portion of the emulsion displays separate layers of oil, which indicates clog. Pipette tip F indicates a clog (separate oil layers) and wetting failure (non-uniform emulsion). |
Emulsion failures are not expected to be observed across the entire chip. The above images are for illustrative purposes to show a range in emulsion failures. A clog is not expected to impact other lanes on the chip. |
## Impacted |
Tab 1 Text |
Tab 2 Text |
Tab 3 Text |
Tab 4 Text |
Tab 5 Text |
Tab 6 Text STEP |
<!-- image --> |
2.1a |
After transfer of the GEMs + Recovery Agent |
2.1b After aspiration of Recovery Agent/ Partitioning Oil |
2.1d After addition of Dynabeads Cleanup Mix |
## Normal |
<!-- image --> |
All liquid levels are similar in the aqueous sample volume (clear) and Recovery Agent/ Partitioning Oil (pink). |
<!-- image --> |
All liquid volumes are similar in the aqueous sample volume (clear) and residual Recovery Agent/Partitioning Oil (pink). |
All liquid volumes are similar after addition of the Dynabeads Cleanup Mix. |
<!-- image --> |
## Impacted |
<!-- image --> |
Tube G indicates a reagent clog has occurred. There is a decreased volume of aqueous layer (clear). |
Tube C and E indicate a wetting failure has occurred. |
There is an abnormal volume of Recovery Agent/Partitioning Oil (pink). |
<!-- image --> |
Tube G indicates a reagent clog has occurred. There is a decreased volume of aqueous layer (clear). There is also a greater residual volume of Recovery Agent/ Partitioning Oil (pink). |
Tube C and E indicate a wetting failure has occurred. There is an abnormal residual volume of Recovery Agent/Partitioning Oil (pink). |
<!-- image --> |
Tube G indicates a reagent clog has occurred. There is an abnormal ratio of Dynabeads Cleanup Mix (brown) to Recovery Agent/Partitioning Oil (appears white). Tube C and E indicate a wetting failure has occurred. There is an abnormal ratio of Dynabeads Cleanup Mix (brown) to Recovery Agent/Partitioning Oil (appears whit... |
If a channel clogs or wetting failure occurs during GEM generation, it is recommended that the sample be remade. If any of the listed issues occur, take a picture and send it to support@10xgenomics.com for further assistance. Replacement reagents and chips may be provided for properly documented clogs or wetting fail... |
Tab 1 Text |
Tab 2 Text |
Tab 3 Text |
Tab 4 Text |
Tab 5 Text |
Tab 6 Text Tab 1 Text Tab 2 Text Tab 3 Text Tab 4 Text Tab 5 Text Tab 6 Text |
<!-- image --> |
## Chromium X Series Errors |
The Chromium X touchscreen will guide the user through recoverable errors. If the error continues, or if the instrument has seen critical or intermediate errors, email support@10xgenomics.com with the displayed error code. Support will request a troubleshooting package. Upload pertinent logs to 10x Genomics by navigati... |
## There are two types of errors: |
Critical Errors - When the instrument has seen a critical error, the run will immediately abort. Do not proceed with any further runs. Contact support@10xgenomics.com with the error code. |
- a. System Error |
- b. Pressure Error |
- c. Chip Error |
- d. Run Error |
- e. Temperature Error |
- f. Software Error |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.