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User Recoverable Errors - Follow error handling instructions through the touchscreen and continue the run. |
- a. Gasket Error |
- b. Tray Error |
- c. Chip Error |
- d. Unsupported Chip Error |
- e. Network Error |
- f. Update Error |
Consult the Chromium X Series Instrument User Guide with Readiness Test (CG000396) for additional information and follow the instrument touchscreen prompts for execution. The instrument touchscreen will guide the user through recoverable errors . |
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## Appendix |
| GEM-X Chip Loading Overview | 73 | |
|----------------------------------------------------------|------| |
| Post Library Construction Quantification using KAPA qPCR | 74 | |
| LabChip Traces | 75 | |
| Oligonucleotide Sequences | 76 | |
## GEM-X Chip Loading Overview |
This section provides a quick overview to the GEM-X chip loading and does not include detailed instructions. Refer to Load GEM-X Chip on page 36 for details. The Chromium X/iX Chip Holder and X/iX Chip Gasket images shown here are representative. Refer to Assemble GEM-X Chip on page 32 for details. |
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## Post Library Construction Quantification using KAPA qPCR |
- a. Thaw KAPA Library Quantification Kit for Illumina Platforms. |
- b. Dilute 2 μl sample with deionized water to appropriate dilutions that fall within the linear detection range of the KAPA Library Quantification Kit for Illumina Platforms. (For more accurate quantification, make the dilution(s) in duplicate). |
- c. Make enough Quantification Master Mix for the DNA dilutions per sample and the DNA Standards (plus 10% excess) using the guidance for 1 reaction volume below. |
- d. Dispense 16 μl Quantification Master Mix for sample dilutions and DNA Standards into a 96 well PCR plate. |
- e. Add 4 μl sample dilutions and 4 μl DNA Standards to appropriate wells. Centrifuge briefly. |
- f. Incubate in a thermal cycler with the following protocol. |
- g. Follow the manufacturer's recommendations for qPCR-based quantification. For library quantification for sequencer clustering, determine the concentration based on insert size derived from the Bioanalyzer/TapeStation trace. |
| Quantification Master Mix | 1X ( μ l) | |
|-------------------------------|-------------| |
| SYBR Fast Master Mix + Primer | 12 | |
| Water | 4 | |
| Total | 16 | |
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| Step | Temperature | Run Time | |
|--------|-------------------------------------|------------| |
| 1 | 95°C | 00:03:00 | |
| 2 | 95°C | 00:00:05 | |
| 3 | 67°C Read Signal | 00:00:30 | |
| 4 | Go to Step 2, 29X (Total 30 cycles) | | |
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