Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
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 Community
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2
INTRODUCTION
1
9
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
In addition to possessing high affinity and specificity for their targets, aptamers can be synthesized chemically and thus are attractive reagents for use in therapeutic and other applications where quality control is critical.
[ "9", "10", "13", "14" ]
227
3,000
0
false
In addition to possessing high affinity and specificity for their targets, aptamers can be synthesized chemically and thus are attractive reagents for use in therapeutic and other applications where quality control is critical.
[]
In addition to possessing high affinity and specificity for their targets, aptamers can be synthesized chemically and thus are attractive reagents for use in therapeutic and other applications where quality control is critical.
true
true
true
true
true
506
2
INTRODUCTION
1
9
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
Aptamers targeting surface antigens as well as whole cells have previously been selected (10–13).
[ "9", "10", "13", "14" ]
97
3,001
0
false
Aptamers targeting surface antigens as well as whole cells have previously been selected.
[ "10–13" ]
Aptamers targeting surface antigens as well as whole cells have previously been selected.
true
true
true
true
true
506
2
INTRODUCTION
1
9
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
More importantly, it has recently been reported that nucleic acids selected to bind to a cell surface marker, prostate-specific membrane antigen, can themselves be internalized.
[ "9", "10", "13", "14" ]
177
3,002
0
false
More importantly, it has recently been reported that nucleic acids selected to bind to a cell surface marker, prostate-specific membrane antigen, can themselves be internalized.
[]
More importantly, it has recently been reported that nucleic acids selected to bind to a cell surface marker, prostate-specific membrane antigen, can themselves be internalized.
true
true
true
true
true
506
2
INTRODUCTION
1
14
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
These anti-PSMA aptamers have been shown to be capable of carrying a nanoparticle into cells expressing this antigen (14).
[ "9", "10", "13", "14" ]
122
3,003
1
false
These anti-PSMA aptamers have been shown to be capable of carrying a nanoparticle into cells expressing this antigen.
[ "14" ]
These anti-PSMA aptamers have been shown to be capable of carrying a nanoparticle into cells expressing this antigen.
true
true
true
true
true
506
2
INTRODUCTION
1
9
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
Building on these findings, we wished to determine whether anti-PSMA aptamers could also deliver functional siRNA molecules to a cell.
[ "9", "10", "13", "14" ]
134
3,004
0
false
Building on these findings, we wished to determine whether anti-PSMA aptamers could also deliver functional siRNA molecules to a cell.
[]
Building on these findings, we wished to determine whether anti-PSMA aptamers could also deliver functional siRNA molecules to a cell.
true
true
true
true
true
506
2
INTRODUCTION
1
9
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
To do this we have generated an aptamer:streptavidin:siRNA conjugate.
[ "9", "10", "13", "14" ]
69
3,005
0
false
To do this we have generated an aptamer:streptavidin:siRNA conjugate.
[]
To do this we have generated an aptamer:streptavidin:siRNA conjugate.
true
true
true
true
true
506
2
INTRODUCTION
1
9
[ "b9", "b10", "b13", "b14" ]
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
Delivery using these aptamer conjugates was found to be efficient and specific for cells expressing the PSMA antigen.
[ "9", "10", "13", "14" ]
117
3,006
0
false
Delivery using these aptamer conjugates was found to be efficient and specific for cells expressing the PSMA antigen.
[]
Delivery using these aptamer conjugates was found to be efficient and specific for cells expressing the PSMA antigen.
true
true
true
true
true
506
0
DISCUSSION
0
null
null
16,740,739
pmid-16478693|pmid-16296791|pmid-15538359|pmid-15990405|pmid-12771197
The development of nucleic acid reagents that can be used to internalize biomolecular cargoes is not only novel, but also extremely practical.
null
142
3,007
0
false
null
null
The development of nucleic acid reagents that can be used to internalize biomolecular cargoes is not only novel, but also extremely practical.
true
true
true
true
true
507
0
DISCUSSION
0
null
null
16,740,739
pmid-16478693|pmid-16296791|pmid-15538359|pmid-15990405|pmid-12771197
Unlike lipid amalgams, nucleic acids can target specific cell types.
null
68
3,008
0
false
null
null
Unlike lipid amalgams, nucleic acids can target specific cell types.
true
true
true
true
true
507
0
DISCUSSION
0
null
null
16,740,739
pmid-16478693|pmid-16296791|pmid-15538359|pmid-15990405|pmid-12771197
Unlike peptides, conjugation chemistry between nucleic acid partners can potentially involve either simple hybridization or co-synthesis.
null
137
3,009
0
false
null
null
Unlike peptides, conjugation chemistry between nucleic acid partners can potentially involve either simple hybridization or co-synthesis.
true
true
true
true
true
507
0
DISCUSSION
0
null
null
16,740,739
pmid-16478693|pmid-16296791|pmid-15538359|pmid-15990405|pmid-12771197
While the simplification of conjugation chemistry may not initially seem to be a great advantage, quality control is one of the most time- and labor-intensive portions of pharmaceutical production, and any simplification at this step should result in great savings throughout the process.
null
288
3,010
0
false
null
null
While the simplification of conjugation chemistry may not initially seem to be a great advantage, quality control is one of the most time- and labor-intensive portions of pharmaceutical production, and any simplification at this step should result in great savings throughout the process.
true
true
true
true
true
507
1
DISCUSSION
1
26
[ "b26", "b27", "b28", "b29", "b30", "b31", "b32" ]
16,740,739
pmid-15520458|pmid-16204072|pmid-15908939|pmid-12782573|pmid-10626807|pmid-11166685|pmid-16199376|pmid-14996706|pmid-10647616|pmid-11344318
We have used streptavdin as a non-covalent linker primarily because of its ease of use and modularity.
[ "26", "27", "28", "29", "30", "31", "32" ]
102
3,011
0
false
We have used streptavdin as a non-covalent linker primarily because of its ease of use and modularity.
[]
We have used streptavdin as a non-covalent linker primarily because of its ease of use and modularity.
true
true
true
true
true
508
1
DISCUSSION
1
30
[ "b26", "b27", "b28", "b29", "b30", "b31", "b32" ]
16,740,739
pmid-15520458|pmid-16204072|pmid-15908939|pmid-12782573|pmid-10626807|pmid-11166685|pmid-16199376|pmid-14996706|pmid-10647616|pmid-11344318
While streptavidin conjugates and fusion proteins have previously been used as imaging agents (26,27) and for the targeted delivery of therapeutics in whole animals (28,29) as well as in clinical trials (30), such conjugates have also been shown to elicit an immune response (31).
[ "26", "27", "28", "29", "30", "31", "32" ]
280
3,012
1
false
While streptavidin conjugates and fusion proteins have previously been used as imaging agents and for the targeted delivery of therapeutics in whole animals as well as in clinical trials, such conjugates have also been shown to elicit an immune response.
[ "26,27", "28,29", "30", "31" ]
While streptavidin conjugates and fusion proteins have previously been used as imaging agents and for the targeted delivery of therapeutics in whole animals as well as in clinical trials, such conjugates have also been shown to elicit an immune response.
true
true
true
true
true
508
1
DISCUSSION
1
32
[ "b26", "b27", "b28", "b29", "b30", "b31", "b32" ]
16,740,739
pmid-15520458|pmid-16204072|pmid-15908939|pmid-12782573|pmid-10626807|pmid-11166685|pmid-16199376|pmid-14996706|pmid-10647616|pmid-11344318
Therefore, immunogenicity may limit the use of streptavidin as a delivery vehicle for siRNAs, at least until less immunogenic streptavidin variants can be identified (32).
[ "26", "27", "28", "29", "30", "31", "32" ]
171
3,013
1
false
Therefore, immunogenicity may limit the use of streptavidin as a delivery vehicle for siRNAs, at least until less immunogenic streptavidin variants can be identified.
[ "32" ]
Therefore, immunogenicity may limit the use of streptavidin as a delivery vehicle for siRNAs, at least until less immunogenic streptavidin variants can be identified.
true
true
true
true
true
508
1
DISCUSSION
1
26
[ "b26", "b27", "b28", "b29", "b30", "b31", "b32" ]
16,740,739
pmid-15520458|pmid-16204072|pmid-15908939|pmid-12782573|pmid-10626807|pmid-11166685|pmid-16199376|pmid-14996706|pmid-10647616|pmid-11344318
It may also be possible to conjugate aptamers to siRNAs via linkers other than streptavidin, including by simple co-synthesis or hybridization.
[ "26", "27", "28", "29", "30", "31", "32" ]
143
3,014
0
false
It may also be possible to conjugate aptamers to siRNAs via linkers other than streptavidin, including by simple co-synthesis or hybridization.
[]
It may also be possible to conjugate aptamers to siRNAs via linkers other than streptavidin, including by simple co-synthesis or hybridization.
true
true
true
true
true
508
2
DISCUSSION
0
null
null
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
Nonetheless, there are some advantages to modular conjugation via a streptavidin bridge that we have developed here.
null
116
3,015
0
false
null
null
Nonetheless, there are some advantages to modular conjugation via a streptavidin bridge that we have developed here.
true
true
true
true
true
509
2
DISCUSSION
0
null
null
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
For example, the use of streptavidin may provide a convenient platform for further dissecting siRNA and microRNA processing machinery in vivo.
null
142
3,016
0
false
null
null
For example, the use of streptavidin may provide a convenient platform for further dissecting siRNA and microRNA processing machinery in vivo.
true
true
true
true
true
509
2
DISCUSSION
0
null
null
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
It seems likely that the siRNAs must be removed from streptavidin prior to entry into RNA-initiated silencing complexes.
null
120
3,017
0
false
null
null
It seems likely that the siRNAs must be removed from streptavidin prior to entry into RNA-initiated silencing complexes.
true
true
true
true
true
509
2
DISCUSSION
0
null
null
16,740,739
pmid-11032850|pmid-11279054|pmid-9501188|pmid-15520166
By varying the linker length and chemistry between the biotin moiety and the siRNA, it may be possible to probe the initial processing steps for siRNAs separate from downstream assembly steps and gene regulation.
null
212
3,018
0
false
null
null
By varying the linker length and chemistry between the biotin moiety and the siRNA, it may be possible to probe the initial processing steps for siRNAs separate from downstream assembly steps and gene regulation.
true
true
true
true
true
509
3
DISCUSSION
0
null
null
16,740,739
null
Importantly, since streptavidin is a tetramer and has four functional binding sites for biotin, internalizing and therapeutic nucleic acids can be mixed and matched.
null
165
3,019
0
false
null
null
Importantly, since streptavidin is a tetramer and has four functional binding sites for biotin, internalizing and therapeutic nucleic acids can be mixed and matched.
true
true
true
true
true
510
3
DISCUSSION
0
null
null
16,740,739
null
This will be especially important for the future development of siRNA therapeutics and reagents.
null
96
3,020
0
false
null
null
This will be especially important for the future development of siRNA therapeutics and reagents.
true
true
true
true
true
510
3
DISCUSSION
0
null
null
16,740,739
null
By using the streptavidin bridge and a judicious mixture of siRNAs, the uniform delivery of multiple different siRNA sequences to a given cell can be ensured.
null
158
3,021
0
false
null
null
By using the streptavidin bridge and a judicious mixture of siRNAs, the uniform delivery of multiple different siRNA sequences to a given cell can be ensured.
true
true
true
true
true
510
3
DISCUSSION
0
null
null
16,740,739
null
Moreover, it seems that the development of simple transfection reagents for use in systems biology applications may now be a realistic possibility.
null
147
3,022
0
false
null
null
Moreover, it seems that the development of simple transfection reagents for use in systems biology applications may now be a realistic possibility.
true
true
true
true
true
510
3
DISCUSSION
0
null
null
16,740,739
null
Aptamers could be pre-appended to streptavidin, and these conjugates in turn mixed with any set or library of biotinylated siRNAs in order to make a one-step, ready-to-use transfection reagent.
null
193
3,023
0
false
null
null
Aptamers could be pre-appended to streptavidin, and these conjugates in turn mixed with any set or library of biotinylated siRNAs in order to make a one-step, ready-to-use transfection reagent.
true
true
true
true
true
510
3
DISCUSSION
0
null
null
16,740,739
null
The simplicity of such a method relative to current procedures (such as frequently irreproducible transfections with lipid reagents or series of complex packaging steps with viral vectors) would greatly expand the use of large siRNA sets in many molecular biology applications.
null
277
3,024
0
false
null
null
The simplicity of such a method relative to current procedures (such as frequently irreproducible transfections with lipid reagents or series of complex packaging steps with viral vectors) would greatly expand the use of large siRNA sets in many molecular biology applications.
true
true
true
true
true
510
4
DISCUSSION
1
33
[ "b33", "b34", "b10", "b13", "b35" ]
16,740,739
pmid-12750292|pmid-14528023|pmid-11279054|pmid-9501188|pmid-10198434
While our current results are limited to LNCaP cells which naturally express PSMA, the internalization of an anti-PSMA antibody has previously been observed for a number of other cell lines that have been engineered to stably or transiently express PSMA (33,34).
[ "33", "34", "10", "13", "35" ]
262
3,025
0
false
While our current results are limited to LNCaP cells which naturally express PSMA, the internalization of an anti-PSMA antibody has previously been observed for a number of other cell lines that have been engineered to stably or transiently express PSMA.
[ "33,34" ]
While our current results are limited to LNCaP cells which naturally express PSMA, the internalization of an anti-PSMA antibody has previously been observed for a number of other cell lines that have been engineered to stably or transiently express PSMA.
true
true
true
true
true
511
4
DISCUSSION
1
33
[ "b33", "b34", "b10", "b13", "b35" ]
16,740,739
pmid-12750292|pmid-14528023|pmid-11279054|pmid-9501188|pmid-10198434
Thus it seems likely that the aptamer-mediated delivery of siRNA may prove useful for any cell type that normally expresses PSMA or that can be engineered to express PSMA.
[ "33", "34", "10", "13", "35" ]
171
3,026
0
false
Thus it seems likely that the aptamer-mediated delivery of siRNA may prove useful for any cell type that normally expresses PSMA or that can be engineered to express PSMA.
[]
Thus it seems likely that the aptamer-mediated delivery of siRNA may prove useful for any cell type that normally expresses PSMA or that can be engineered to express PSMA.
true
true
true
true
true
511
4
DISCUSSION
1
33
[ "b33", "b34", "b10", "b13", "b35" ]
16,740,739
pmid-12750292|pmid-14528023|pmid-11279054|pmid-9501188|pmid-10198434
Moreover, since aptamers can be selected to bind a wide variety of cells (10–13,35), we envision that additional, cell-specific transfection reagents will be developed and that the method described here for prostate tumor cells may become a general strategy for the specific delivery of siRNA to almost any cell type.
[ "33", "34", "10", "13", "35" ]
317
3,027
0
false
Moreover, since aptamers can be selected to bind a wide variety of cells, we envision that additional, cell-specific transfection reagents will be developed and that the method described here for prostate tumor cells may become a general strategy for the specific delivery of siRNA to almost any cell type.
[ "10–13,35" ]
Moreover, since aptamers can be selected to bind a wide variety of cells, we envision that additional, cell-specific transfection reagents will be developed and that the method described here for prostate tumor cells may become a general strategy for the specific delivery of siRNA to almost any cell type.
true
true
true
true
true
511
0
INTRODUCTION
1
1
[ "b1", "b2", "b3" ]
16,990,248
pmid-12573856|pmid-1962210|NA
For the investigation of structural and functional aspects of nucleic acids, mainly synthetic primer or probe molecules are being used that have the basic chemical structure of naturally occurring DNA or RNA.
[ "1", "2", "3" ]
208
3,028
0
false
For the investigation of structural and functional aspects of nucleic acids, mainly synthetic primer or probe molecules are being used that have the basic chemical structure of naturally occurring DNA or RNA.
[]
For the investigation of structural and functional aspects of nucleic acids, mainly synthetic primer or probe molecules are being used that have the basic chemical structure of naturally occurring DNA or RNA.
true
true
true
true
true
512
0
INTRODUCTION
1
1
[ "b1", "b2", "b3" ]
16,990,248
pmid-12573856|pmid-1962210|NA
Although derivatives have been synthesized that exhibit particular characteristics, such as locked nucleic acid (LNA) (1) with its improved duplex stability, the molecules still exhibit the basic conformation of natural nucleic acids.
[ "1", "2", "3" ]
234
3,029
1
false
Although derivatives have been synthesized that exhibit particular characteristics, such as locked nucleic acid (LNA) with its improved duplex stability, the molecules still exhibit the basic conformation of natural nucleic acids.
[ "1" ]
Although derivatives have been synthesized that exhibit particular characteristics, such as locked nucleic acid (LNA) with its improved duplex stability, the molecules still exhibit the basic conformation of natural nucleic acids.
true
true
true
true
true
512
0
INTRODUCTION
1
2
[ "b1", "b2", "b3" ]
16,990,248
pmid-12573856|pmid-1962210|NA
An exception to this is peptide nucleic acid (PNA), which is a synthetic DNA-mimic that is based on an amide rather than sugar-phosphate backbone (2).
[ "1", "2", "3" ]
150
3,030
1
false
An exception to this is peptide nucleic acid (PNA), which is a synthetic DNA-mimic that is based on an amide rather than sugar-phosphate backbone.
[ "2" ]
An exception to this is peptide nucleic acid (PNA), which is a synthetic DNA-mimic that is based on an amide rather than sugar-phosphate backbone.
true
true
true
true
true
512
0
INTRODUCTION
1
3
[ "b1", "b2", "b3" ]
16,990,248
pmid-12573856|pmid-1962210|NA
While PNA behaves similar to normal nucleic acids, it simultaneously has several distinct properties (3) because of its elementary structural difference.
[ "1", "2", "3" ]
153
3,031
1
false
While PNA behaves similar to normal nucleic acids, it simultaneously has several distinct properties because of its elementary structural difference.
[ "3" ]
While PNA behaves similar to normal nucleic acids, it simultaneously has several distinct properties because of its elementary structural difference.
true
true
true
true
true
512
0
INTRODUCTION
1
1
[ "b1", "b2", "b3" ]
16,990,248
pmid-12573856|pmid-1962210|NA
As yet, PNA has found only limited use.
[ "1", "2", "3" ]
39
3,032
0
false
As yet, PNA has found only limited use.
[]
As yet, PNA has found only limited use.
true
true
true
true
true
512
1
INTRODUCTION
1
4
[ "b4", "b7", "b8", "b9", "b10", "b11", "b12" ]
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Another artificial molecule, which has attracted even less attention than PNA, is the l-enantiomer of DNA (Figure 1).
[ "4", "7", "8", "9", "10", "11", "12" ]
117
3,033
0
false
Another artificial molecule, which has attracted even less attention than PNA, is the l-enantiomer of DNA (Figure 1).
[]
Another artificial molecule, which has attracted even less attention than PNA, is the l-enantiomer of DNA (Figure 1).
true
true
true
true
true
513
1
INTRODUCTION
1
4
[ "b4", "b7", "b8", "b9", "b10", "b11", "b12" ]
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
In principle, L-DNA is identical to the natural d-conformation but for the fact that it is an exact mirror-image of natural DNA and forms a left-turning double-helix upon hybridization to a complementary L-DNA sequence (4–7).
[ "4", "7", "8", "9", "10", "11", "12" ]
225
3,034
0
false
In principle, L-DNA is identical to the natural d-conformation but for the fact that it is an exact mirror-image of natural DNA and forms a left-turning double-helix upon hybridization to a complementary L-DNA sequence.
[ "4–7" ]
In principle, L-DNA is identical to the natural d-conformation but for the fact that it is an exact mirror-image of natural DNA and forms a left-turning double-helix upon hybridization to a complementary L-DNA sequence.
true
true
true
true
true
513
1
INTRODUCTION
1
8
[ "b4", "b7", "b8", "b9", "b10", "b11", "b12" ]
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
L-DNA was examined as a potential antisense reagent but failed to perform adequately (8).
[ "4", "7", "8", "9", "10", "11", "12" ]
89
3,035
1
false
L-DNA was examined as a potential antisense reagent but failed to perform adequately.
[ "8" ]
L-DNA was examined as a potential antisense reagent but failed to perform adequately.
true
true
true
true
true
513
1
INTRODUCTION
1
9
[ "b4", "b7", "b8", "b9", "b10", "b11", "b12" ]
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Because of its reduced sensitivity to nucleases (9), L-DNA might be an interesting molecule for the creation of aptamer libraries (10).
[ "4", "7", "8", "9", "10", "11", "12" ]
135
3,036
1
false
Because of its reduced sensitivity to nucleases, L-DNA might be an interesting molecule for the creation of aptamer libraries.
[ "9", "10" ]
Because of its reduced sensitivity to nucleases, L-DNA might be an interesting molecule for the creation of aptamer libraries.
true
true
true
true
true
513
1
INTRODUCTION
1
4
[ "b4", "b7", "b8", "b9", "b10", "b11", "b12" ]
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Overall, however, little use has been made of this artificial form of nucleic acid.
[ "4", "7", "8", "9", "10", "11", "12" ]
83
3,037
0
false
Overall, however, little use has been made of this artificial form of nucleic acid.
[]
Overall, however, little use has been made of this artificial form of nucleic acid.
true
true
true
true
true
513
1
INTRODUCTION
1
4
[ "b4", "b7", "b8", "b9", "b10", "b11", "b12" ]
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Only very recently, the use of L-DNA as a tag molecule was demonstrated (11,12).
[ "4", "7", "8", "9", "10", "11", "12" ]
80
3,038
0
false
Only very recently, the use of L-DNA as a tag molecule was demonstrated.
[ "11,12" ]
Only very recently, the use of L-DNA as a tag molecule was demonstrated.
true
true
true
true
true
513
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
We came across L-DNA when contemplating the design of a microarray platform that is not assaying one specific biological issue at a time—such as transcription profiles, genotypes or protein–DNA interactions, respectively—but would allow a simultaneous analysis of all these and other aspects in a single experiment.
[ "13", "14" ]
315
3,039
0
false
We came across L-DNA when contemplating the design of a microarray platform that is not assaying one specific biological issue at a time—such as transcription profiles, genotypes or protein–DNA interactions, respectively—but would allow a simultaneous analysis of all these and other aspects in a single experiment.
[]
We came across L-DNA when contemplating the design of a microarray platform that is not assaying one specific biological issue at a time—such as transcription profiles, genotypes or protein–DNA interactions, respectively—but would allow a simultaneous analysis of all these and other aspects in a single experiment.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Such an array would allow combining different kinds of molecular markers for a more accurate and informative diagnosis, for example.
[ "13", "14" ]
132
3,040
0
false
Such an array would allow combining different kinds of molecular markers for a more accurate and informative diagnosis, for example.
[]
Such an array would allow combining different kinds of molecular markers for a more accurate and informative diagnosis, for example.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Especially for analyses on samples of limited quantity, simultaneous assaying might become important.
[ "13", "14" ]
101
3,041
0
false
Especially for analyses on samples of limited quantity, simultaneous assaying might become important.
[]
Especially for analyses on samples of limited quantity, simultaneous assaying might become important.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
For many assays, it would also be advantageous to perform the actual reaction in homogenous solution rather than on a solid support, since the presence of a surface influences many reactions negatively.
[ "13", "14" ]
202
3,042
0
false
For many assays, it would also be advantageous to perform the actual reaction in homogenous solution rather than on a solid support, since the presence of a surface influences many reactions negatively.
[]
For many assays, it would also be advantageous to perform the actual reaction in homogenous solution rather than on a solid support, since the presence of a surface influences many reactions negatively.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
The establishment of a universal ZIP-code microarray (13,14) could solve these problems.
[ "13", "14" ]
88
3,043
0
false
The establishment of a universal ZIP-code microarray could solve these problems.
[ "13,14" ]
The establishment of a universal ZIP-code microarray could solve these problems.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Universal microarrays contain a set of unique and distinct (ZIP-code) oligonucleotides that should not have any complementary sequence in any organism and are made solely for the purpose of addressing with a complementary oligonucleotide a particular location on a microarray (Figure 2).
[ "13", "14" ]
287
3,044
0
false
Universal microarrays contain a set of unique and distinct (ZIP-code) oligonucleotides that should not have any complementary sequence in any organism and are made solely for the purpose of addressing with a complementary oligonucleotide a particular location on a microarray (Figure 2).
[]
Universal microarrays contain a set of unique and distinct (ZIP-code) oligonucleotides that should not have any complementary sequence in any organism and are made solely for the purpose of addressing with a complementary oligonucleotide a particular location on a microarray (Figure 2).
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
The oligonucleotides should have similar thermodynamic properties so that hybridization can be performed at one experimental condition with identical stringency.
[ "13", "14" ]
161
3,045
0
false
The oligonucleotides should have similar thermodynamic properties so that hybridization can be performed at one experimental condition with identical stringency.
[]
The oligonucleotides should have similar thermodynamic properties so that hybridization can be performed at one experimental condition with identical stringency.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Instead of having to produce many different microarrays, a single design can be used for a variety of assays.
[ "13", "14" ]
109
3,046
0
false
Instead of having to produce many different microarrays, a single design can be used for a variety of assays.
[]
Instead of having to produce many different microarrays, a single design can be used for a variety of assays.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
The actual analysis is carried out with a mixture of probe or primer molecules in homogenous solution.
[ "13", "14" ]
102
3,047
0
false
The actual analysis is carried out with a mixture of probe or primer molecules in homogenous solution.
[]
The actual analysis is carried out with a mixture of probe or primer molecules in homogenous solution.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Each oligonucleotide of the mixture is composed of an assay-specific sequence portion that is linked to a distinct, ZIP-code complementary tag-sequence.
[ "13", "14" ]
152
3,048
0
false
Each oligonucleotide of the mixture is composed of an assay-specific sequence portion that is linked to a distinct, ZIP-code complementary tag-sequence.
[]
Each oligonucleotide of the mixture is composed of an assay-specific sequence portion that is linked to a distinct, ZIP-code complementary tag-sequence.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Only subsequent to the analysis-reaction, the molecules are physically separated by hybridization to the ZIP-code microarray and therefore made available to individual signal scoring.
[ "13", "14" ]
183
3,049
0
false
Only subsequent to the analysis-reaction, the molecules are physically separated by hybridization to the ZIP-code microarray and therefore made available to individual signal scoring.
[]
Only subsequent to the analysis-reaction, the molecules are physically separated by hybridization to the ZIP-code microarray and therefore made available to individual signal scoring.
true
true
true
true
true
514
2
INTRODUCTION
1
13
[ "b13", "b14" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
All probe molecules could assay the same kind of information, such as transcript levels for example, or different types of analysis could be combined.
[ "13", "14" ]
150
3,050
0
false
All probe molecules could assay the same kind of information, such as transcript levels for example, or different types of analysis could be combined.
[]
All probe molecules could assay the same kind of information, such as transcript levels for example, or different types of analysis could be combined.
true
true
true
true
true
514
3
INTRODUCTION
0
null
null
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
However, the aspect of avoiding tag-sequences that exhibit similarity to any genome is difficult to achieve.
null
108
3,051
0
false
null
null
However, the aspect of avoiding tag-sequences that exhibit similarity to any genome is difficult to achieve.
true
true
true
true
true
515
3
INTRODUCTION
0
null
null
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Worse, even very short sequence homologies already lead to some cross-hybridization and thus a sequence-dependent accumulation of background signal, if complex samples are hybridized.
null
183
3,052
0
false
null
null
Worse, even very short sequence homologies already lead to some cross-hybridization and thus a sequence-dependent accumulation of background signal, if complex samples are hybridized.
true
true
true
true
true
515
3
INTRODUCTION
0
null
null
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
L-DNA could solve this problem, since its duplexes turn left while natural D-DNA double-helices turn right.
null
107
3,053
0
false
null
null
L-DNA could solve this problem, since its duplexes turn left while natural D-DNA double-helices turn right.
true
true
true
true
true
515
3
INTRODUCTION
0
null
null
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Also in terms of stability in an impure environment, L-DNA microarrays could be superior.
null
89
3,054
0
false
null
null
Also in terms of stability in an impure environment, L-DNA microarrays could be superior.
true
true
true
true
true
515
3
INTRODUCTION
0
null
null
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
On this basis, we studied elementary characteristics of L-DNA and describe how this DNA-conformation can be utilized for the generation of a universal microarray platform for a simultaneous analysis of different molecular parameters in a single experiment.
null
256
3,055
0
false
null
null
On this basis, we studied elementary characteristics of L-DNA and describe how this DNA-conformation can be utilized for the generation of a universal microarray platform for a simultaneous analysis of different molecular parameters in a single experiment.
true
true
true
true
true
515
3
INTRODUCTION
0
null
null
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
However, also other applications of L-DNA are discussed in view of its biophysical and biochemical properties.
null
110
3,056
0
false
null
null
However, also other applications of L-DNA are discussed in view of its biophysical and biochemical properties.
true
true
true
true
true
515
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
L-DNA is a versatile but yet mostly ignored form of nucleic acid.
null
65
3,057
0
false
null
null
L-DNA is a versatile but yet mostly ignored form of nucleic acid.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
Because of its chemical compatibility with the normal D-form of DNA and its identical biophysical behaviour, but for the mirror-image mode, much information can be copied from the vast amount of knowledge on the functioning of D-DNA.
null
233
3,058
0
false
null
null
Because of its chemical compatibility with the normal D-form of DNA and its identical biophysical behaviour, but for the mirror-image mode, much information can be copied from the vast amount of knowledge on the functioning of D-DNA.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
Its different chirality, however, offers many possible applications in molecular biology.
null
89
3,059
0
false
null
null
Its different chirality, however, offers many possible applications in molecular biology.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
Here, we demonstrate its utilization toward the establishment of an improved and universally applicable microarray platform.
null
124
3,060
0
false
null
null
Here, we demonstrate its utilization toward the establishment of an improved and universally applicable microarray platform.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
The ZIP-code scheme is not new as such and yet, utilizing L-DNA oligonucleotides for this purpose improves the concept considerably.
null
132
3,061
0
false
null
null
The ZIP-code scheme is not new as such and yet, utilizing L-DNA oligonucleotides for this purpose improves the concept considerably.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
One important advantage, for example, is the freedom in designing the oligonucleotide sequences, since there is no need to avoid homology to naturally occurring sequences.
null
171
3,062
0
false
null
null
One important advantage, for example, is the freedom in designing the oligonucleotide sequences, since there is no need to avoid homology to naturally occurring sequences.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
Therefore, levelling of the dissociation temperatures is simpler to achieve, since the hybridization conditions on an L-DNA microarray can be modified across a wider spectrum of parameters, and many more target molecules can be analysed in parallel.
null
249
3,063
0
false
null
null
Therefore, levelling of the dissociation temperatures is simpler to achieve, since the hybridization conditions on an L-DNA microarray can be modified across a wider spectrum of parameters, and many more target molecules can be analysed in parallel.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
For the lack of any cross-hybridization between L-DNA and D-DNA, also overall background is reduced.
null
100
3,064
0
false
null
null
For the lack of any cross-hybridization between L-DNA and D-DNA, also overall background is reduced.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
In experiments with D-DNA oligonucleotides—also in standard assays—background is produced also via an interaction of target molecules with only a relatively short portion of the probes.
null
185
3,065
0
false
null
null
In experiments with D-DNA oligonucleotides—also in standard assays—background is produced also via an interaction of target molecules with only a relatively short portion of the probes.
true
true
true
true
true
516
0
DISCUSSION
0
null
null
16,990,248
pmid-12573856|pmid-1962210|NA
Already homologous hexamer sequences can produce considerable background signals, if target is in large excess.
null
111
3,066
0
false
null
null
Already homologous hexamer sequences can produce considerable background signals, if target is in large excess.
true
true
true
true
true
516
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
In addition to the assays shown in the result section, there are more that can take advantage of the ZIP-code approach.
null
119
3,067
0
false
null
null
In addition to the assays shown in the result section, there are more that can take advantage of the ZIP-code approach.
true
true
true
true
true
517
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Since PNA does bind to L-DNA in a sequence-specific manner, for instance, it is possible to synthesize peptides to which during the synthesis—and by the identical chemical process—a PNA-tag is attached.
null
202
3,068
0
false
null
null
Since PNA does bind to L-DNA in a sequence-specific manner, for instance, it is possible to synthesize peptides to which during the synthesis—and by the identical chemical process—a PNA-tag is attached.
true
true
true
true
true
517
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Similar to the transcription factor analysis, the interaction of peptides with analytes could be studied in homogenous solution for purposes such as protein–protein interaction assays or epitope mapping.
null
203
3,069
0
false
null
null
Similar to the transcription factor analysis, the interaction of peptides with analytes could be studied in homogenous solution for purposes such as protein–protein interaction assays or epitope mapping.
true
true
true
true
true
517
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
For detection, the molecules would be separated by the hybridization specificity of the PNA-tag.
null
96
3,070
0
false
null
null
For detection, the molecules would be separated by the hybridization specificity of the PNA-tag.
true
true
true
true
true
517
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Another interesting application could be the use of aptamers as ligand binder reagents.
null
87
3,071
0
false
null
null
Another interesting application could be the use of aptamers as ligand binder reagents.
true
true
true
true
true
517
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Known (D-DNA) aptamer molecules could be synthesized with a unique L-DNA tag attached.
null
86
3,072
0
false
null
null
Known (D-DNA) aptamer molecules could be synthesized with a unique L-DNA tag attached.
true
true
true
true
true
517
1
DISCUSSION
0
null
null
16,990,248
NA|NA|pmid-8414968|pmid-8011650|pmid-9326601|NA|NA
Alternatively, new libraries made entirely of L-DNA—including an L-DNA ZIP-code sequence—could be generated.
null
108
3,073
0
false
null
null
Alternatively, new libraries made entirely of L-DNA—including an L-DNA ZIP-code sequence—could be generated.
true
true
true
true
true
517
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Since the ZIP-coded assay reaction is performed in solution, sensitivity can be increased by repetition.
[ "9" ]
104
3,074
0
false
Since the ZIP-coded assay reaction is performed in solution, sensitivity can be increased by repetition.
[]
Since the ZIP-coded assay reaction is performed in solution, sensitivity can be increased by repetition.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
By cycling a polymerase reaction, for example, more primer molecules will be binding to the template and incorporate label.
[ "9" ]
123
3,075
0
false
By cycling a polymerase reaction, for example, more primer molecules will be binding to the template and incorporate label.
[]
By cycling a polymerase reaction, for example, more primer molecules will be binding to the template and incorporate label.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Therefore, a higher percentage of the molecules hybridizing to the array will carry a label and contribute to the signal produced.
[ "9" ]
130
3,076
0
false
Therefore, a higher percentage of the molecules hybridizing to the array will carry a label and contribute to the signal produced.
[]
Therefore, a higher percentage of the molecules hybridizing to the array will carry a label and contribute to the signal produced.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Another important element for utilization of microarrays in a routine manner is the aspect of stability.
[ "9" ]
104
3,077
0
false
Another important element for utilization of microarrays in a routine manner is the aspect of stability.
[]
Another important element for utilization of microarrays in a routine manner is the aspect of stability.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
While standard arrays are rather long-living in a dry environment, stability is drastically reduced in the presence of biological samples.
[ "9" ]
138
3,078
0
false
While standard arrays are rather long-living in a dry environment, stability is drastically reduced in the presence of biological samples.
[]
While standard arrays are rather long-living in a dry environment, stability is drastically reduced in the presence of biological samples.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
The resistance of L-DNA nucleotides to enzymatic degradation had been investigated before using oligonucleotides of mixed L- and D-nucleotides (9).
[ "9" ]
147
3,079
1
false
The resistance of L-DNA nucleotides to enzymatic degradation had been investigated before using oligonucleotides of mixed L- and D-nucleotides.
[ "9" ]
The resistance of L-DNA nucleotides to enzymatic degradation had been investigated before using oligonucleotides of mixed L- and D-nucleotides.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
We could demonstrate that this effect is strong in both single-strand and double-strand molecules for both exonucleolytic and endonucleolytic digestion with common enzymes, failing to detect any apparent degradation.
[ "9" ]
216
3,080
0
false
We could demonstrate that this effect is strong in both single-strand and double-strand molecules for both exonucleolytic and endonucleolytic digestion with common enzymes, failing to detect any apparent degradation.
[]
We could demonstrate that this effect is strong in both single-strand and double-strand molecules for both exonucleolytic and endonucleolytic digestion with common enzymes, failing to detect any apparent degradation.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
The use of L-DNA could therefore allow the positioning of microarrays in a fluidic system through which there is a continuous flow of biological material.
[ "9" ]
154
3,081
0
false
The use of L-DNA could therefore allow the positioning of microarrays in a fluidic system through which there is a continuous flow of biological material.
[]
The use of L-DNA could therefore allow the positioning of microarrays in a fluidic system through which there is a continuous flow of biological material.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
Apart from handling advantages, more molecules could be captured in a prolonged incubation in a continuous flow system, thus accumulating signal.
[ "9" ]
145
3,082
0
false
Apart from handling advantages, more molecules could be captured in a prolonged incubation in a continuous flow system, thus accumulating signal.
[]
Apart from handling advantages, more molecules could be captured in a prolonged incubation in a continuous flow system, thus accumulating signal.
true
true
true
true
true
518
2
DISCUSSION
1
9
[ "b9" ]
16,990,248
pmid-10493873|pmid-8944025|pmid-8011650
This could be important for analysing fermentation or production processes, for example, and fits well with currently ongoing developments toward small-scale lab-on-chip devices.
[ "9" ]
178
3,083
0
false
This could be important for analysing fermentation or production processes, for example, and fits well with currently ongoing developments toward small-scale lab-on-chip devices.
[]
This could be important for analysing fermentation or production processes, for example, and fits well with currently ongoing developments toward small-scale lab-on-chip devices.
true
true
true
true
true
518
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
The production of chimeric molecules could be simplified by linking D-formed primer libraries with L-formed tag libraries enzymatically.
[ "22", "23", "24", "25" ]
136
3,084
0
false
The production of chimeric molecules could be simplified by linking D-formed primer libraries with L-formed tag libraries enzymatically.
[]
The production of chimeric molecules could be simplified by linking D-formed primer libraries with L-formed tag libraries enzymatically.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Thereby, the ZIP-code libraries could be utilized for all kinds of assays and organisms.
[ "22", "23", "24", "25" ]
88
3,085
0
false
Thereby, the ZIP-code libraries could be utilized for all kinds of assays and organisms.
[]
Thereby, the ZIP-code libraries could be utilized for all kinds of assays and organisms.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Only the D-formed primer had to be synthesized anew.
[ "22", "23", "24", "25" ]
52
3,086
0
false
Only the D-formed primer had to be synthesized anew.
[]
Only the D-formed primer had to be synthesized anew.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Adding to the 3′-terminus of the L-DNA and the 5′ end of the D-DNA molecules a few common D-formed nucleotides, for instance three each, ligation could be used to link the molecules in presence of a commonly added complementary hexamer.
[ "22", "23", "24", "25" ]
236
3,087
0
false
Adding to the 3′-terminus of the L-DNA and the 5′ end of the D-DNA molecules a few common D-formed nucleotides, for instance three each, ligation could be used to link the molecules in presence of a commonly added complementary hexamer.
[]
Adding to the 3′-terminus of the L-DNA and the 5′ end of the D-DNA molecules a few common D-formed nucleotides, for instance three each, ligation could be used to link the molecules in presence of a commonly added complementary hexamer.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
The mixing of the numerous D- and L-formed oligonucleotides could be accomplished robotically.
[ "22", "23", "24", "25" ]
94
3,088
0
false
The mixing of the numerous D- and L-formed oligonucleotides could be accomplished robotically.
[]
The mixing of the numerous D- and L-formed oligonucleotides could be accomplished robotically.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Alternatively, we intend to utilize recent developments in synthetic biology and synthesize the chimeric oligonucleotides on chip surfaces, cleave them off the support, before eluting and using them in subsequent experiments (22,23).
[ "22", "23", "24", "25" ]
233
3,089
0
false
Alternatively, we intend to utilize recent developments in synthetic biology and synthesize the chimeric oligonucleotides on chip surfaces, cleave them off the support, before eluting and using them in subsequent experiments.
[ "22,23" ]
Alternatively, we intend to utilize recent developments in synthetic biology and synthesize the chimeric oligonucleotides on chip surfaces, cleave them off the support, before eluting and using them in subsequent experiments.
true
true
true
true
true
519
3
DISCUSSION
1
24
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
We use the system of febit biotech (24) that permits a micromirror-based in situ synthesis of oligonucleotides, similar to the NimbleGen system (25) but for the fact that synthesis takes place in microfluidic channels.
[ "22", "23", "24", "25" ]
218
3,090
1
false
We use the system of febit biotech that permits a micromirror-based in situ synthesis of oligonucleotides, similar to the NimbleGen system but for the fact that synthesis takes place in microfluidic channels.
[ "24", "25" ]
We use the system of febit biotech that permits a micromirror-based in situ synthesis of oligonucleotides, similar to the NimbleGen system but for the fact that synthesis takes place in microfluidic channels.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Instead of four phosphoramidites, eight are required.
[ "22", "23", "24", "25" ]
53
3,091
0
false
Instead of four phosphoramidites, eight are required.
[]
Instead of four phosphoramidites, eight are required.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
The monomers with the respective photolabile groups are available.
[ "22", "23", "24", "25" ]
66
3,092
0
false
The monomers with the respective photolabile groups are available.
[]
The monomers with the respective photolabile groups are available.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
As a matter of fact, the system already provides connections for eight bottles.
[ "22", "23", "24", "25" ]
79
3,093
0
false
As a matter of fact, the system already provides connections for eight bottles.
[]
As a matter of fact, the system already provides connections for eight bottles.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Therefore, such synthesis does not require any changes at all.
[ "22", "23", "24", "25" ]
62
3,094
0
false
Therefore, such synthesis does not require any changes at all.
[]
Therefore, such synthesis does not require any changes at all.
true
true
true
true
true
519
3
DISCUSSION
1
22
[ "b22", "b23", "b24", "b25" ]
16,990,248
pmid-8954553|pmid-15616567|pmid-14627841|pmid-10504697
Many different molecules could be synthesized cheaply since in small quantities.
[ "22", "23", "24", "25" ]
80
3,095
0
false
Many different molecules could be synthesized cheaply since in small quantities.
[]
Many different molecules could be synthesized cheaply since in small quantities.
true
true
true
true
true
519
4
DISCUSSION
0
null
null
16,990,248
null
The current costs for L-DNA amidites is around 10 times as high as for standard D-DNA amidites.
null
95
3,096
0
false
null
null
The current costs for L-DNA amidites is around 10 times as high as for standard D-DNA amidites.
true
true
true
true
true
520
4
DISCUSSION
0
null
null
16,990,248
null
However, this price is based on small-scale synthesis of L-DNA amidites.
null
72
3,097
0
false
null
null
However, this price is based on small-scale synthesis of L-DNA amidites.
true
true
true
true
true
520
4
DISCUSSION
0
null
null
16,990,248
null
Since all the precursors are chiral enantiomers of D-DNA, there is no need to change or re-develop standard synthesis or analysis protocols.
null
140
3,098
0
false
null
null
Since all the precursors are chiral enantiomers of D-DNA, there is no need to change or re-develop standard synthesis or analysis protocols.
true
true
true
true
true
520
4
DISCUSSION
0
null
null
16,990,248
null
The only change is the starting L-ribofuranose instead of the D-ribofurnose.
null
76
3,099
0
false
null
null
The only change is the starting L-ribofuranose instead of the D-ribofurnose.
true
true
true
true
true
520