Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
xet
 Community
1
paragraph_index
int64
sec
string
p_has_citation
int64
cites
string
citeids
list
pmid
int64
cited_id
string
sentences
string
all_sent_cites
list
sent_len
int64
sentence_batch_index
int64
sent_has_citation
float64
qc_fail
bool
cited_sentence
string
cites_in_sentence
list
cln_sentence
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is_cap
bool
is_alpha
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ends_wp
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cit_qc
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lgtm
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__index_level_0__
int64
5
DISCUSSION
1
43
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Disruption of the RAD51 gene leads to cell death (43), whereas RAD51 paralogs including XRCC3 are not essential for cell viability.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
131
6,800
1
false
Disruption of the RAD51 gene leads to cell death, whereas RAD51 paralogs including XRCC3 are not essential for cell viability.
[ "43" ]
Disruption of the RAD51 gene leads to cell death, whereas RAD51 paralogs including XRCC3 are not essential for cell viability.
true
true
true
true
true
1,112
5
DISCUSSION
1
44
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
The XRCC3-deficient X-ray sensitive hamster cell line, irs1SF shows increased chromosome mis-segregation (44) and has a 25-fold decrease in the frequency of error-free homology-directed repair of DNA DSBs (38).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
210
6,801
1
false
The XRCC3-deficient X-ray sensitive hamster cell line, irs1SF shows increased chromosome mis-segregation and has a 25-fold decrease in the frequency of error-free homology-directed repair of DNA DSBs.
[ "44", "38" ]
The XRCC3-deficient X-ray sensitive hamster cell line, irs1SF shows increased chromosome mis-segregation and has a 25-fold decrease in the frequency of error-free homology-directed repair of DNA DSBs.
true
true
true
true
true
1,112
5
DISCUSSION
1
44
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Both can be restored to wild-type levels through XRCC3 expression (44).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
71
6,802
1
false
Both can be restored to wild-type levels through XRCC3 expression.
[ "44" ]
Both can be restored to wild-type levels through XRCC3 expression.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
However, in contrast to RAD51, XRCC3 is known to act in the early as well as late stages of HR (45,46).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
103
6,803
0
false
However, in contrast to RAD51, XRCC3 is known to act in the early as well as late stages of HR.
[ "45,46" ]
However, in contrast to RAD51, XRCC3 is known to act in the early as well as late stages of HR.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Following reduction of XRCC3 mRNA levels we observed nearly complete elimination of rAAV-mediated gene targeting, which may reflect the multiple roles XRCC3 plays in HR.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
169
6,804
0
false
Following reduction of XRCC3 mRNA levels we observed nearly complete elimination of rAAV-mediated gene targeting, which may reflect the multiple roles XRCC3 plays in HR.
[]
Following reduction of XRCC3 mRNA levels we observed nearly complete elimination of rAAV-mediated gene targeting, which may reflect the multiple roles XRCC3 plays in HR.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Although the reduction in XRCC3 mRNA was about 80% compared to mRNA levels in non-silenced cells, the remaining XRCC3 protein may be below a critical concentration.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
164
6,805
0
false
Although the reduction in XRCC3 mRNA was about 80% compared to mRNA levels in non-silenced cells, the remaining XRCC3 protein may be below a critical concentration.
[]
Although the reduction in XRCC3 mRNA was about 80% compared to mRNA levels in non-silenced cells, the remaining XRCC3 protein may be below a critical concentration.
true
true
true
true
true
1,112
5
DISCUSSION
1
47
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
A complex between XRCC3 and another RAD51 paralog, RAD51C associates specifically with single-stranded DNA (47) and modulates replication fork progression by slowing it down after DNA damage (48).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
196
6,806
1
false
A complex between XRCC3 and another RAD51 paralog, RAD51C associates specifically with single-stranded DNA and modulates replication fork progression by slowing it down after DNA damage.
[ "47", "48" ]
A complex between XRCC3 and another RAD51 paralog, RAD51C associates specifically with single-stranded DNA and modulates replication fork progression by slowing it down after DNA damage.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
The complex also plays a role probably downstream of RAD54 in the HR pathway, i.e.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
82
6,807
0
false
The complex also plays a role probably downstream of RAD54 in the HR pathway, i.e.
[]
The complex also plays a role probably downstream of RAD54 in the HR pathway, i.e.
true
true
true
true
true
1,112
5
DISCUSSION
1
49
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
as a resolvase of Holliday junctions (49).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
42
6,808
1
false
as a resolvase of Holliday junctions.
[ "49" ]
as a resolvase of Holliday junctions.
false
true
true
true
false
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Thus, resolution of recombination intermediates and/or triggering of DNA damage-related signaling present two of the possible roles for the XRCC3/RAD51C complex in rAAV targeting.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
179
6,809
0
false
Thus, resolution of recombination intermediates and/or triggering of DNA damage-related signaling present two of the possible roles for the XRCC3/RAD51C complex in rAAV targeting.
[]
Thus, resolution of recombination intermediates and/or triggering of DNA damage-related signaling present two of the possible roles for the XRCC3/RAD51C complex in rAAV targeting.
true
true
true
true
true
1,112
5
DISCUSSION
1
45
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
A recent report reveals that XRCC3 forms nuclear foci independently of RAD51 very early during homology-directed DSB repair, associates directly with DNA, and promotes formation of the RAD51 nucleoprotein filament (45).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
219
6,810
1
false
A recent report reveals that XRCC3 forms nuclear foci independently of RAD51 very early during homology-directed DSB repair, associates directly with DNA, and promotes formation of the RAD51 nucleoprotein filament.
[ "45" ]
A recent report reveals that XRCC3 forms nuclear foci independently of RAD51 very early during homology-directed DSB repair, associates directly with DNA, and promotes formation of the RAD51 nucleoprotein filament.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
One may hypothesize, that the absence of XRCC3 affects potential binding of RAD51 to the single-stranded AAV vector genome and subsequently its homologous pairing with the chromosomal locus during the targeting reaction.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
220
6,811
0
false
One may hypothesize, that the absence of XRCC3 affects potential binding of RAD51 to the single-stranded AAV vector genome and subsequently its homologous pairing with the chromosomal locus during the targeting reaction.
[]
One may hypothesize, that the absence of XRCC3 affects potential binding of RAD51 to the single-stranded AAV vector genome and subsequently its homologous pairing with the chromosomal locus during the targeting reaction.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Based on this hypothesis we propose a molecular model for the mechanism of rAAV-mediated targeting in human cells (Figure 8).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
125
6,812
0
false
Based on this hypothesis we propose a molecular model for the mechanism of rAAV-mediated targeting in human cells (Figure 8).
[]
Based on this hypothesis we propose a molecular model for the mechanism of rAAV-mediated targeting in human cells (Figure 8).
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Removal of capsid proteins exposes the single-stranded AAV genome, allowing its degradation by nucleases.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
105
6,813
0
false
Removal of capsid proteins exposes the single-stranded AAV genome, allowing its degradation by nucleases.
[]
Removal of capsid proteins exposes the single-stranded AAV genome, allowing its degradation by nucleases.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
There are several processes that could prevent this degradation: annealing of positive and negative viral genomes, second-strand synthesis, or coating of the single-stranded region by high-affinity single-stranded DNA-binding proteins, such as RPA.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
248
6,814
0
false
There are several processes that could prevent this degradation: annealing of positive and negative viral genomes, second-strand synthesis, or coating of the single-stranded region by high-affinity single-stranded DNA-binding proteins, such as RPA.
[]
There are several processes that could prevent this degradation: annealing of positive and negative viral genomes, second-strand synthesis, or coating of the single-stranded region by high-affinity single-stranded DNA-binding proteins, such as RPA.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
RPA, a heterotrimer with multiple roles in DNA replication and repair, may ensure proper initiation of HR by preventing secondary structure formation from single-stranded DNA, thus supporting even coating by RAD51.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
214
6,815
0
false
RPA, a heterotrimer with multiple roles in DNA replication and repair, may ensure proper initiation of HR by preventing secondary structure formation from single-stranded DNA, thus supporting even coating by RAD51.
[]
RPA, a heterotrimer with multiple roles in DNA replication and repair, may ensure proper initiation of HR by preventing secondary structure formation from single-stranded DNA, thus supporting even coating by RAD51.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
It is unknown, however, whether RPA affects the structure of the rAAV ITRs.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
75
6,816
0
false
It is unknown, however, whether RPA affects the structure of the rAAV ITRs.
[]
It is unknown, however, whether RPA affects the structure of the rAAV ITRs.
true
true
true
true
true
1,112
5
DISCUSSION
1
50
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
The RAD51C/XRCC3 heterodimer binds single-stranded AAV DNA at a terminal repeat site that resembles a DNA break (50), and through direct interactions between XRCC3 and RAD51 mediates the formation of the RAD51 nucleoprotein filament.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
233
6,817
1
false
The RAD51C/XRCC3 heterodimer binds single-stranded AAV DNA at a terminal repeat site that resembles a DNA break, and through direct interactions between XRCC3 and RAD51 mediates the formation of the RAD51 nucleoprotein filament.
[ "50" ]
The RAD51C/XRCC3 heterodimer binds single-stranded AAV DNA at a terminal repeat site that resembles a DNA break, and through direct interactions between XRCC3 and RAD51 mediates the formation of the RAD51 nucleoprotein filament.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
RAD51 is likely derived from a mobile pool or from a BRCA2 complex, which also has a high binding affinity for ds- to single-stranded DNA transitions (51,52).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
158
6,818
0
false
RAD51 is likely derived from a mobile pool or from a BRCA2 complex, which also has a high binding affinity for ds- to single-stranded DNA transitions.
[ "51,52" ]
RAD51 is likely derived from a mobile pool or from a BRCA2 complex, which also has a high binding affinity for ds- to single-stranded DNA transitions.
true
true
true
true
true
1,112
5
DISCUSSION
1
53
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
Following ATP binding and hydrolysis by XRCC3 the RAD51C/XRCC3 dimer may be destabilized (53).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
94
6,819
1
false
Following ATP binding and hydrolysis by XRCC3 the RAD51C/XRCC3 dimer may be destabilized.
[ "53" ]
Following ATP binding and hydrolysis by XRCC3 the RAD51C/XRCC3 dimer may be destabilized.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
and probably RAD54B interact with the RAD51 filament, unwind the ds genomic target DNA, and may remove nucleosomes from the homologous locus in order to prepare for pairing with the rAAV DNA.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
191
6,820
0
false
and probably RAD54B interact with the RAD51 filament, unwind the ds genomic target DNA, and may remove nucleosomes from the homologous locus in order to prepare for pairing with the rAAV DNA.
[]
and probably RAD54B interact with the RAD51 filament, unwind the ds genomic target DNA, and may remove nucleosomes from the homologous locus in order to prepare for pairing with the rAAV DNA.
false
true
true
true
false
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
After pairing with the homologous target the resulting intermediate can be resolved either by crossing over and Holliday junction resolution or by the predominant pathway for DSB repair in mammalian cells—non-crossover gene conversion (54,55).
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
243
6,821
0
false
After pairing with the homologous target the resulting intermediate can be resolved either by crossing over and Holliday junction resolution or by the predominant pathway for DSB repair in mammalian cells—non-crossover gene conversion.
[ "54,55" ]
After pairing with the homologous target the resulting intermediate can be resolved either by crossing over and Holliday junction resolution or by the predominant pathway for DSB repair in mammalian cells—non-crossover gene conversion.
true
true
true
true
true
1,112
5
DISCUSSION
1
35
[ "b35", "b43", "b44", "b38", "b44", "b45", "b46", "b47", "b48", "b49", "b45", "b50", "b51", "b52", "b53", "b54", "b55" ]
16,822,856
pmid-12456786|pmid-8943369|pmid-11025669|pmid-3347204|pmid-11025669|pmid-15372620|pmid-12191483|pmid-11751635|pmid-12718895|pmid-14716019|pmid-15372620|pmid-11459987|pmid-12228710|pmid-15703751|pmid-15037616|pmid-10880452|pmid-11356153
The rAAV DNA serves as a template for repair of the chromosomal target, however, gene conversion can occur on the virus with the genomic sequence as a template.
[ "35", "43", "44", "38", "44", "45", "46", "47", "48", "49", "45", "50", "51", "52", "53", "54", "55" ]
160
6,822
0
false
The rAAV DNA serves as a template for repair of the chromosomal target, however, gene conversion can occur on the virus with the genomic sequence as a template.
[]
The rAAV DNA serves as a template for repair of the chromosomal target, however, gene conversion can occur on the virus with the genomic sequence as a template.
true
true
true
true
true
1,112
6
DISCUSSION
1
56
[ "b56" ]
16,822,856
pmid-8319297
Our system shares significant similarity with a yeast model for gene targeting where donation of information to an unbroken chromosome is facilitated by a linearized plasmid (56).
[ "56" ]
179
6,823
1
false
Our system shares significant similarity with a yeast model for gene targeting where donation of information to an unbroken chromosome is facilitated by a linearized plasmid.
[ "56" ]
Our system shares significant similarity with a yeast model for gene targeting where donation of information to an unbroken chromosome is facilitated by a linearized plasmid.
true
true
true
true
true
1,113
6
DISCUSSION
1
56
[ "b56" ]
16,822,856
pmid-8319297
The authors suggest this process occurs via the formation of a heteroduplex intermediate and the efficiency of donation depends on the spatial distribution of heteroduplex DNA.
[ "56" ]
176
6,824
0
false
The authors suggest this process occurs via the formation of a heteroduplex intermediate and the efficiency of donation depends on the spatial distribution of heteroduplex DNA.
[]
The authors suggest this process occurs via the formation of a heteroduplex intermediate and the efficiency of donation depends on the spatial distribution of heteroduplex DNA.
true
true
true
true
true
1,113
6
DISCUSSION
1
56
[ "b56" ]
16,822,856
pmid-8319297
The frequency of donation was proportional to the increased distance from the break to the mutation and always resulted from a single gene conversion event, which is very likely to be the case with rAAV targeting.
[ "56" ]
213
6,825
0
false
The frequency of donation was proportional to the increased distance from the break to the mutation and always resulted from a single gene conversion event, which is very likely to be the case with rAAV targeting.
[]
The frequency of donation was proportional to the increased distance from the break to the mutation and always resulted from a single gene conversion event, which is very likely to be the case with rAAV targeting.
true
true
true
true
true
1,113
7
DISCUSSION
1
30
[ "b30", "b57", "b58", "b59", "b58" ]
16,822,856
pmid-15047832|pmid-15871683|pmid-12897142|pmid-9032269|pmid-12897142
The conclusion that rAAV targeting depends on HR is in concert with recent evidence that targeting occurs with higher frequency in actively replicating cells, presumably the S phase of the cell cycle (30,57).
[ "30", "57", "58", "59", "58" ]
208
6,826
0
false
The conclusion that rAAV targeting depends on HR is in concert with recent evidence that targeting occurs with higher frequency in actively replicating cells, presumably the S phase of the cell cycle.
[ "30,57" ]
The conclusion that rAAV targeting depends on HR is in concert with recent evidence that targeting occurs with higher frequency in actively replicating cells, presumably the S phase of the cell cycle.
true
true
true
true
true
1,114
7
DISCUSSION
1
58
[ "b30", "b57", "b58", "b59", "b58" ]
16,822,856
pmid-15047832|pmid-15871683|pmid-12897142|pmid-9032269|pmid-12897142
NHEJ and HR function in a complementary, overlapping manner (58) and the cell cycle phase determines the prevalence of one or the other repair process.
[ "30", "57", "58", "59", "58" ]
151
6,827
1
false
NHEJ and HR function in a complementary, overlapping manner and the cell cycle phase determines the prevalence of one or the other repair process.
[ "58" ]
NHEJ and HR function in a complementary, overlapping manner and the cell cycle phase determines the prevalence of one or the other repair process.
true
true
true
true
true
1,114
7
DISCUSSION
1
58
[ "b30", "b57", "b58", "b59", "b58" ]
16,822,856
pmid-15047832|pmid-15871683|pmid-12897142|pmid-9032269|pmid-12897142
A predominant role for NHEJ early in the cell cycle in mammalian cells is supported for example by the high IR sensitivity of NHEJ-defective cells in the G1 phase (59), while HR seems to occur mostly during S and G2/M phases (58).
[ "30", "57", "58", "59", "58" ]
230
6,828
1
false
A predominant role for NHEJ early in the cell cycle in mammalian cells is supported for example by the high IR sensitivity of NHEJ-defective cells in the G1 phase, while HR seems to occur mostly during S and G2/M phases.
[ "59", "58" ]
A predominant role for NHEJ early in the cell cycle in mammalian cells is supported for example by the high IR sensitivity of NHEJ-defective cells in the G1 phase, while HR seems to occur mostly during S and G2/M phases.
true
true
true
true
true
1,114
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
A notable finding in our study is the significant decrease of GFP positive cells within 5 days after infection.
[ "29", "16", "19", "30", "60", "61" ]
111
6,829
0
false
A notable finding in our study is the significant decrease of GFP positive cells within 5 days after infection.
[]
A notable finding in our study is the significant decrease of GFP positive cells within 5 days after infection.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
This phenomenon has not yet been reported.
[ "29", "16", "19", "30", "60", "61" ]
42
6,830
0
false
This phenomenon has not yet been reported.
[]
This phenomenon has not yet been reported.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
Some of the rAAV targeting systems measured the number of events after an extended period of antibiotic selection (29).
[ "29", "16", "19", "30", "60", "61" ]
119
6,831
1
false
Some of the rAAV targeting systems measured the number of events after an extended period of antibiotic selection.
[ "29" ]
Some of the rAAV targeting systems measured the number of events after an extended period of antibiotic selection.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
Other systems are based on staining of the cells at a particular time point after infection (16,19).
[ "29", "16", "19", "30", "60", "61" ]
100
6,832
0
false
Other systems are based on staining of the cells at a particular time point after infection.
[ "16,19" ]
Other systems are based on staining of the cells at a particular time point after infection.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
In yet another system, which uses GFP as a readout in living cells, the results were given for only one time point, i.e.
[ "29", "16", "19", "30", "60", "61" ]
120
6,833
0
false
In yet another system, which uses GFP as a readout in living cells, the results were given for only one time point, i.e.
[]
In yet another system, which uses GFP as a readout in living cells, the results were given for only one time point, i.e.
true
true
true
true
true
1,115
8
DISCUSSION
1
30
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
as positive foci at day 7 (30).
[ "29", "16", "19", "30", "60", "61" ]
31
6,834
1
false
as positive foci at day 7.
[ "30" ]
as positive foci at day 7.
false
true
true
true
false
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
The higher number of GFP positive cells at early time-points could also imply asymmetric recognition of the rAAV ITRs by a component of the cellular recombination machinery.
[ "29", "16", "19", "30", "60", "61" ]
173
6,835
0
false
The higher number of GFP positive cells at early time-points could also imply asymmetric recognition of the rAAV ITRs by a component of the cellular recombination machinery.
[]
The higher number of GFP positive cells at early time-points could also imply asymmetric recognition of the rAAV ITRs by a component of the cellular recombination machinery.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
That is, gene conversion on the viral vector instead of on the genomic target may have occurred.
[ "29", "16", "19", "30", "60", "61" ]
96
6,836
0
false
That is, gene conversion on the viral vector instead of on the genomic target may have occurred.
[]
That is, gene conversion on the viral vector instead of on the genomic target may have occurred.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
This may happen, for example, if a nick occurs at a hot-spot on the viral DNA within the double-stranded (60,61) or single-stranded region with subsequent invasion and copying of the chromosomal DNA sequence to repair the vector mutation.
[ "29", "16", "19", "30", "60", "61" ]
238
6,837
0
false
This may happen, for example, if a nick occurs at a hot-spot on the viral DNA within the double-stranded or single-stranded region with subsequent invasion and copying of the chromosomal DNA sequence to repair the vector mutation.
[ "60,61" ]
This may happen, for example, if a nick occurs at a hot-spot on the viral DNA within the double-stranded or single-stranded region with subsequent invasion and copying of the chromosomal DNA sequence to repair the vector mutation.
true
true
true
true
true
1,115
8
DISCUSSION
1
29
[ "b29", "b16", "b19", "b30", "b60", "b61" ]
16,822,856
pmid-9537413|pmid-12724413|pmid-10775597|pmid-15047832|pmid-15208627|pmid-15731255
The unusual structure of the ITRs and the ss–ds transitional regions may be likely targets for nucleases.
[ "29", "16", "19", "30", "60", "61" ]
105
6,838
0
false
The unusual structure of the ITRs and the ss–ds transitional regions may be likely targets for nucleases.
[]
The unusual structure of the ITRs and the ss–ds transitional regions may be likely targets for nucleases.
true
true
true
true
true
1,115
9
DISCUSSION
1
62
[ "b62", "b63", "b64" ]
16,822,856
pmid-12941339|pmid-12140331|pmid-10455437
Our results together with previous data provide the groundwork for improvement of the rAAV targeting frequency by shifting the balance from the NHEJ to the HR pathway.
[ "62", "63", "64" ]
167
6,839
0
false
Our results together with previous data provide the groundwork for improvement of the rAAV targeting frequency by shifting the balance from the NHEJ to the HR pathway.
[]
Our results together with previous data provide the groundwork for improvement of the rAAV targeting frequency by shifting the balance from the NHEJ to the HR pathway.
true
true
true
true
true
1,116
9
DISCUSSION
1
62
[ "b62", "b63", "b64" ]
16,822,856
pmid-12941339|pmid-12140331|pmid-10455437
Since DNA-PK does not affect gene targeting, specific inhibition of DNA-PK with chemically synthesized small molecules (62), which has been shown to enhance gene conversion (63), combined with overexpression of proteins promoting HR (64) or synchronization of cells in S phase (for targeting of stem-cells) would enhance...
[ "62", "63", "64" ]
406
6,840
1
false
Since DNA-PK does not affect gene targeting, specific inhibition of DNA-PK with chemically synthesized small molecules, which has been shown to enhance gene conversion, combined with overexpression of proteins promoting HR or synchronization of cells in S phase (for targeting of stem-cells) would enhance further target...
[ "62", "63", "64" ]
Since DNA-PK does not affect gene targeting, specific inhibition of DNA-PK with chemically synthesized small molecules, which has been shown to enhance gene conversion, combined with overexpression of proteins promoting HR or synchronization of cells in S phase (for targeting of stem-cells) would enhance further target...
true
true
true
true
true
1,116
0
INTRODUCTION
1
1
[ "B1", "B2", "B3", "B4 B5 B6 B7", "B8", "B9", "B10", "B11" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
The influenza A viruses are members of the family Orthomyxoviridae with a genome consisting of eight single-stranded RNA segments of negative polarity.
[ "1", "2", "3", "4–7", "8", "9", "10", "11" ]
151
6,841
0
false
The influenza A viruses are members of the family Orthomyxoviridae with a genome consisting of eight single-stranded RNA segments of negative polarity.
[]
The influenza A viruses are members of the family Orthomyxoviridae with a genome consisting of eight single-stranded RNA segments of negative polarity.
true
true
true
true
true
1,117
0
INTRODUCTION
1
1
[ "B1", "B2", "B3", "B4 B5 B6 B7", "B8", "B9", "B10", "B11" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Each one is encapsidated by binding to the polymerase complex and to a number of nucleoprotein (NP) monomers (1), forming a ribonucleoprotein (RNP) complex (2).
[ "1", "2", "3", "4–7", "8", "9", "10", "11" ]
160
6,842
1
false
Each one is encapsidated by binding to the polymerase complex and to a number of nucleoprotein (NP) monomers, forming a ribonucleoprotein (RNP) complex.
[ "1", "2" ]
Each one is encapsidated by binding to the polymerase complex and to a number of nucleoprotein (NP) monomers, forming a ribonucleoprotein (RNP) complex.
true
true
true
true
true
1,117
0
INTRODUCTION
1
1
[ "B1", "B2", "B3", "B4 B5 B6 B7", "B8", "B9", "B10", "B11" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Transcription and replication of each virus RNP take place in the nucleus of the infected cell [reviewed in (3)] and hence viral mRNAs are potential substrates for the cellular splicing machinery and need to be exported from the nucleus to express the viral proteins.
[ "1", "2", "3", "4–7", "8", "9", "10", "11" ]
267
6,843
0
false
Transcription and replication of each virus RNP take place in the nucleus of the infected cell and hence viral mRNAs are potential substrates for the cellular splicing machinery and need to be exported from the nucleus to express the viral proteins.
[ "reviewed in (3)" ]
Transcription and replication of each virus RNP take place in the nucleus of the infected cell and hence viral mRNAs are potential substrates for the cellular splicing machinery and need to be exported from the nucleus to express the viral proteins.
true
true
true
true
true
1,117
0
INTRODUCTION
1
4–7
[ "B1", "B2", "B3", "B4 B5 B6 B7", "B8", "B9", "B10", "B11" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Indeed, the collinear transcripts from segments 7 and 8 can be directly expressed or can become spliced to generate M1 and M2 or NS1 and NEP(NS2) proteins, respectively (4–7).
[ "1", "2", "3", "4–7", "8", "9", "10", "11" ]
175
6,844
1
false
Indeed, the collinear transcripts from segments 7 and 8 can be directly expressed or can become spliced to generate M1 and M2 or NS1 and NEP proteins, respectively.
[ "NS2", "4–7" ]
Indeed, the collinear transcripts from segments 7 and 8 can be directly expressed or can become spliced to generate M1 and M2 or NS1 and NEP proteins, respectively.
true
true
true
true
true
1,117
0
INTRODUCTION
1
1
[ "B1", "B2", "B3", "B4 B5 B6 B7", "B8", "B9", "B10", "B11" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
In productively infected cells, these splicing events are regulated in such a way that the steady-state ratios of spliced versus non-spliced mRNAs is only a few percent [reviewed in (8)].
[ "1", "2", "3", "4–7", "8", "9", "10", "11" ]
187
6,845
0
false
In productively infected cells, these splicing events are regulated in such a way that the steady-state ratios of spliced versus non-spliced mRNAs is only a few percent.
[ "reviewed in (8)" ]
In productively infected cells, these splicing events are regulated in such a way that the steady-state ratios of spliced versus non-spliced mRNAs is only a few percent.
true
true
true
true
true
1,117
0
INTRODUCTION
1
9
[ "B1", "B2", "B3", "B4 B5 B6 B7", "B8", "B9", "B10", "B11" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
These relative levels can be modified in non-productive cells (9) or by virus mutation (10) and are not constant along virus infection (11), suggesting the involvement of virus and cellular factors in the regulation of virus mRNA splicing.
[ "1", "2", "3", "4–7", "8", "9", "10", "11" ]
239
6,846
1
false
These relative levels can be modified in non-productive cells or by virus mutation and are not constant along virus infection, suggesting the involvement of virus and cellular factors in the regulation of virus mRNA splicing.
[ "9", "10", "11" ]
These relative levels can be modified in non-productive cells or by virus mutation and are not constant along virus infection, suggesting the involvement of virus and cellular factors in the regulation of virus mRNA splicing.
true
true
true
true
true
1,117
1
INTRODUCTION
1
12–14
[ "B12 B13 B14", "B15", "B16 B17 B18", "B19", "B20", "B21", "B22", "B23", "B24", "B25", "B26", "B24", "B27", "B28", "B29", "B20", "B30 B31 B32", "B33", "B34", "B35" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
The NS1 protein is a crucial regulatory factor during virus infection [reviewed in (12–14)], affecting cellular and viral gene expression and virus counteraction of the interferon response.
[ "12–14", "15", "16–18", "19", "20", "21", "22", "23", "24", "25", "26", "24", "27", "28", "29", "20", "30–32", "33", "34", "35" ]
189
6,847
0
false
The NS1 protein is a crucial regulatory factor during virus infection, affecting cellular and viral gene expression and virus counteraction of the interferon response.
[ "reviewed in (12–14)" ]
The NS1 protein is a crucial regulatory factor during virus infection, affecting cellular and viral gene expression and virus counteraction of the interferon response.
true
true
true
true
true
1,118
1
INTRODUCTION
1
15
[ "B12 B13 B14", "B15", "B16 B17 B18", "B19", "B20", "B21", "B22", "B23", "B24", "B25", "B26", "B24", "B27", "B28", "B29", "B20", "B30 B31 B32", "B33", "B34", "B35" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
It accumulates in the nucleus early during virus infection (15) and when expressed from cloned DNA (16–18), but it can be found in association with polysomes later in the infection (19,20).
[ "12–14", "15", "16–18", "19", "20", "21", "22", "23", "24", "25", "26", "24", "27", "28", "29", "20", "30–32", "33", "34", "35" ]
189
6,848
1
false
It accumulates in the nucleus early during virus infection and when expressed from cloned DNA, but it can be found in association with polysomes later in the infection.
[ "15", "16–18", "19,20" ]
It accumulates in the nucleus early during virus infection and when expressed from cloned DNA, but it can be found in association with polysomes later in the infection.
true
true
true
true
true
1,118
1
INTRODUCTION
1
25
[ "B12 B13 B14", "B15", "B16 B17 B18", "B19", "B20", "B21", "B22", "B23", "B24", "B25", "B26", "B24", "B27", "B28", "B29", "B20", "B30 B31 B32", "B33", "B34", "B35" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
NS1 is a RNA-binding protein that interacts in vitro with dsRNA (21,22), vRNA (23,24), poly-A-containing RNAs (25) and U6 snRNA (26).
[ "12–14", "15", "16–18", "19", "20", "21", "22", "23", "24", "25", "26", "24", "27", "28", "29", "20", "30–32", "33", "34", "35" ]
133
6,849
1
false
NS1 is a RNA-binding protein that interacts in vitro with dsRNA, vRNA, poly-A-containing RNAs and U6 snRNA.
[ "21,22", "23,24", "25", "26" ]
NS1 is a RNA-binding protein that interacts in vitro with dsRNA, vRNA, poly-A-containing RNAs and U6 snRNA.
true
true
true
true
true
1,118
1
INTRODUCTION
1
28
[ "B12 B13 B14", "B15", "B16 B17 B18", "B19", "B20", "B21", "B22", "B23", "B24", "B25", "B26", "B24", "B27", "B28", "B29", "B20", "B30 B31 B32", "B33", "B34", "B35" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
The RNA-binding domain is located within the N-terminal half of the protein (24,27), and the rest of the protein appears to contain an effector domain (28).
[ "12–14", "15", "16–18", "19", "20", "21", "22", "23", "24", "25", "26", "24", "27", "28", "29", "20", "30–32", "33", "34", "35" ]
156
6,850
1
false
The RNA-binding domain is located within the N-terminal half of the protein, and the rest of the protein appears to contain an effector domain.
[ "24,27", "28" ]
The RNA-binding domain is located within the N-terminal half of the protein, and the rest of the protein appears to contain an effector domain.
true
true
true
true
true
1,118
1
INTRODUCTION
1
12–14
[ "B12 B13 B14", "B15", "B16 B17 B18", "B19", "B20", "B21", "B22", "B23", "B24", "B25", "B26", "B24", "B27", "B28", "B29", "B20", "B30 B31 B32", "B33", "B34", "B35" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
NS1 protein interacts with many viral and cellular factors.
[ "12–14", "15", "16–18", "19", "20", "21", "22", "23", "24", "25", "26", "24", "27", "28", "29", "20", "30–32", "33", "34", "35" ]
59
6,851
0
false
NS1 protein interacts with many viral and cellular factors.
[]
NS1 protein interacts with many viral and cellular factors.
true
true
true
true
true
1,118
1
INTRODUCTION
1
29
[ "B12 B13 B14", "B15", "B16 B17 B18", "B19", "B20", "B21", "B22", "B23", "B24", "B25", "B26", "B24", "B27", "B28", "B29", "B20", "B30 B31 B32", "B33", "B34", "B35" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
These include the virus RNP and/or polymerase (29), cellular proteins involved in translation, like hStaufen, PABPI and eIF4G (20,30–32) and cellular factors involved in post-transcriptional processing of RNA, like CPSF (33), PABPII (34) and NS1–BP, a potential splicing-related factor (35).
[ "12–14", "15", "16–18", "19", "20", "21", "22", "23", "24", "25", "26", "24", "27", "28", "29", "20", "30–32", "33", "34", "35" ]
291
6,852
1
false
These include the virus RNP and/or polymerase, cellular proteins involved in translation, like hStaufen, PABPI and eIF4G and cellular factors involved in post-transcriptional processing of RNA, like CPSF, PABPII and NS1–BP, a potential splicing-related factor.
[ "29", "20,30–32", "33", "34", "35" ]
These include the virus RNP and/or polymerase, cellular proteins involved in translation, like hStaufen, PABPI and eIF4G and cellular factors involved in post-transcriptional processing of RNA, like CPSF, PABPII and NS1–BP, a potential splicing-related factor.
true
true
true
true
true
1,118
2
INTRODUCTION
1
36
[ "B36", "B37", "B38", "B16", "B39", "B40", "B41" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
The splicing of NS1 mRNA has been studied in vitro.
[ "36", "37", "38", "16", "39", "40", "41" ]
51
6,853
0
false
The splicing of NS1 mRNA has been studied in vitro.
[]
The splicing of NS1 mRNA has been studied in vitro.
true
true
true
true
true
1,119
2
INTRODUCTION
1
36
[ "B36", "B37", "B38", "B16", "B39", "B40", "B41" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
Early work indicated that it is a non-spliceable transcript under normal in vitro conditions (36), due the block after formation of a 55S pre-splicing complex (37).
[ "36", "37", "38", "16", "39", "40", "41" ]
164
6,854
1
false
Early work indicated that it is a non-spliceable transcript under normal in vitro conditions, due the block after formation of a 55S pre-splicing complex.
[ "36", "37" ]
Early work indicated that it is a non-spliceable transcript under normal in vitro conditions, due the block after formation of a 55S pre-splicing complex.
true
true
true
true
true
1,119
2
INTRODUCTION
1
38
[ "B36", "B37", "B38", "B16", "B39", "B40", "B41" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
Subsequent mapping experiments led to the identification of RNA sequences within NS1 mRNA that may down-regulate its splicing efficiency (38).
[ "36", "37", "38", "16", "39", "40", "41" ]
142
6,855
1
false
Subsequent mapping experiments led to the identification of RNA sequences within NS1 mRNA that may down-regulate its splicing efficiency.
[ "38" ]
Subsequent mapping experiments led to the identification of RNA sequences within NS1 mRNA that may down-regulate its splicing efficiency.
true
true
true
true
true
1,119
2
INTRODUCTION
1
36
[ "B36", "B37", "B38", "B16", "B39", "B40", "B41" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
In vivo studies have also been carried out to analyze the splicing of NS1 mRNA.
[ "36", "37", "38", "16", "39", "40", "41" ]
79
6,856
0
false
In vivo studies have also been carried out to analyze the splicing of NS1 mRNA.
[]
In vivo studies have also been carried out to analyze the splicing of NS1 mRNA.
true
true
true
true
true
1,119
2
INTRODUCTION
1
36
[ "B36", "B37", "B38", "B16", "B39", "B40", "B41" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
When expressed from a RNA PolII-driven cDNA it can be spliced (16,39) and its splicing can be blocked in trans by the expression of the encoded NS1 protein (40,41).
[ "36", "37", "38", "16", "39", "40", "41" ]
164
6,857
0
false
When expressed from a RNA PolII-driven cDNA it can be spliced and its splicing can be blocked in trans by the expression of the encoded NS1 protein.
[ "16,39", "40,41" ]
When expressed from a RNA PolII-driven cDNA it can be spliced and its splicing can be blocked in trans by the expression of the encoded NS1 protein.
true
true
true
true
true
1,119
3
INTRODUCTION
1
25
[ "B25", "B28", "B41", "B33", "B42" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
In addition of the splicing inhibition, PolII-driven expression of NS1 from a cDNA led to a general retention of mRNAs in the nucleus (25,28,41).
[ "25", "28", "41", "33", "42" ]
145
6,858
0
false
In addition of the splicing inhibition, PolII-driven expression of NS1 from a cDNA led to a general retention of mRNAs in the nucleus.
[ "25,28,41" ]
In addition of the splicing inhibition, PolII-driven expression of NS1 from a cDNA led to a general retention of mRNAs in the nucleus.
true
true
true
true
true
1,120
3
INTRODUCTION
1
33
[ "B25", "B28", "B41", "B33", "B42" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
The interaction of NS1 with the CPSF polyadenylation factor (33) suggested a mechanism for this transport block, as NS1–CPSF interaction would inhibit the 3′-processing of cellular transcripts.
[ "25", "28", "41", "33", "42" ]
193
6,859
1
false
The interaction of NS1 with the CPSF polyadenylation factor suggested a mechanism for this transport block, as NS1–CPSF interaction would inhibit the 3′-processing of cellular transcripts.
[ "33" ]
The interaction of NS1 with the CPSF polyadenylation factor suggested a mechanism for this transport block, as NS1–CPSF interaction would inhibit the 3′-processing of cellular transcripts.
true
true
true
true
true
1,120
3
INTRODUCTION
1
42
[ "B25", "B28", "B41", "B33", "B42" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
Furthermore, it was proposed that CPSF interaction would indirectly down-regulate the splicing of cellular, but not viral, single-intron containing transcripts (42).
[ "25", "28", "41", "33", "42" ]
165
6,860
1
false
Furthermore, it was proposed that CPSF interaction would indirectly down-regulate the splicing of cellular, but not viral, single-intron containing transcripts.
[ "42" ]
Furthermore, it was proposed that CPSF interaction would indirectly down-regulate the splicing of cellular, but not viral, single-intron containing transcripts.
true
true
true
true
true
1,120
4
INTRODUCTION
0
null
null
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
The main limitation of these in vivo studies is that NS1 expression was carried out by transfection and the ‘viral’ mRNAs analysed are indeed PolII-driven transcripts.
null
167
6,861
0
false
null
null
The main limitation of these in vivo studies is that NS1 expression was carried out by transfection and the ‘viral’ mRNAs analysed are indeed PolII-driven transcripts.
true
true
true
true
true
1,121
4
INTRODUCTION
0
null
null
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
To avoid these problems we have established a transient virus replication-transcription system in vivo in which a recombinant NS RNP is amplified and transcribed by the viral polymerase.
null
186
6,862
0
false
null
null
To avoid these problems we have established a transient virus replication-transcription system in vivo in which a recombinant NS RNP is amplified and transcribed by the viral polymerase.
true
true
true
true
true
1,121
4
INTRODUCTION
0
null
null
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
Using this more physiological system we show here that NS1 protein down-regulates the splicing and block the nuclear export of its own viral mRNA.
null
146
6,863
0
false
null
null
Using this more physiological system we show here that NS1 protein down-regulates the splicing and block the nuclear export of its own viral mRNA.
true
true
true
true
true
1,121
4
INTRODUCTION
0
null
null
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
Furthermore, we report a genetic analysis of the roles of the various NS1 domains for such regulation.
null
102
6,864
0
false
null
null
Furthermore, we report a genetic analysis of the roles of the various NS1 domains for such regulation.
true
true
true
true
true
1,121
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
In this report, we have analysed the consequences of NS1 protein expression in the splicing and nucleo-cytoplasmic transport of NS transcripts using a transient system for the replication and transcription of a recombinant influenza virus NS replicon.
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
251
6,865
0
false
In this report, we have analysed the consequences of NS1 protein expression in the splicing and nucleo-cytoplasmic transport of NS transcripts using a transient system for the replication and transcription of a recombinant influenza virus NS replicon.
[]
In this report, we have analysed the consequences of NS1 protein expression in the splicing and nucleo-cytoplasmic transport of NS transcripts using a transient system for the replication and transcription of a recombinant influenza virus NS replicon.
true
true
true
true
true
1,122
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
This is a convenient system that can be used as a model of the situation of an infected cell.
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
93
6,866
0
false
This is a convenient system that can be used as a model of the situation of an infected cell.
[]
This is a convenient system that can be used as a model of the situation of an infected cell.
true
true
true
true
true
1,122
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Indeed, the experimental approach used is a development of the amplification in vivo of transcriptionally active viral mini-RNPs employed in the past for structural studies (2,54,55).
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
183
6,867
0
false
Indeed, the experimental approach used is a development of the amplification in vivo of transcriptionally active viral mini-RNPs employed in the past for structural studies.
[ "2,54,55" ]
Indeed, the experimental approach used is a development of the amplification in vivo of transcriptionally active viral mini-RNPs employed in the past for structural studies.
true
true
true
true
true
1,122
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Furthermore, full-length RNPs generated in a similar way have been rescued into infectious virus (44,46).
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
105
6,868
0
false
Furthermore, full-length RNPs generated in a similar way have been rescued into infectious virus.
[ "44,46" ]
Furthermore, full-length RNPs generated in a similar way have been rescued into infectious virus.
true
true
true
true
true
1,122
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Here we show that NS replicons can be transiently amplified and express both NS1 and NEP(NS2) proteins in proportions and to levels similar to those produced in the virus infection (Figures 2–4).
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
195
6,869
0
false
Here we show that NS replicons can be transiently amplified and express both NS1 and NEP proteins in proportions and to levels similar to those produced in the virus infection.
[ "NS2", "Figures 2–4" ]
Here we show that NS replicons can be transiently amplified and express both NS1 and NEP proteins in proportions and to levels similar to those produced in the virus infection.
true
true
true
true
true
1,122
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
We felt that this should be a convenient system to carry out a genetic analysis of the role of NS1 in the maturation and export of viral transcripts as the studies could be made independent of other possible phenotypes described for NS1 mutants.
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
245
6,870
0
false
We felt that this should be a convenient system to carry out a genetic analysis of the role of NS1 in the maturation and export of viral transcripts as the studies could be made independent of other possible phenotypes described for NS1 mutants.
[]
We felt that this should be a convenient system to carry out a genetic analysis of the role of NS1 in the maturation and export of viral transcripts as the studies could be made independent of other possible phenotypes described for NS1 mutants.
true
true
true
true
true
1,122
0
DISCUSSION
1
2
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Thus, mutants NS1 81 and 11C show predominant alterations in late virus gene expression and particle formation (44,46), aspects not relevant in the experimental setting used here.
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
179
6,871
0
false
Thus, mutants NS1 81 and 11C show predominant alterations in late virus gene expression and particle formation, aspects not relevant in the experimental setting used here.
[ "44,46" ]
Thus, mutants NS1 81 and 11C show predominant alterations in late virus gene expression and particle formation, aspects not relevant in the experimental setting used here.
true
true
true
true
true
1,122
0
DISCUSSION
1
56
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
On the other hand, a ΔNS1 virus mutant is difficult to handle in a cell line with a normal interferon response (56) but can be studied normally in a transient situation as the one described here.
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
195
6,872
1
false
On the other hand, a ΔNS1 virus mutant is difficult to handle in a cell line with a normal interferon response but can be studied normally in a transient situation as the one described here.
[ "56" ]
On the other hand, a ΔNS1 virus mutant is difficult to handle in a cell line with a normal interferon response but can be studied normally in a transient situation as the one described here.
true
true
true
true
true
1,122
0
DISCUSSION
1
57
[ "B2", "B54", "B55", "B44", "B46", "B44", "B46", "B56", "B57" ]
17,488,845
pmid-9135141|pmid-11306552|NA|pmid-291039|pmid-6169001|pmid-7407920|pmid-6945577|NA|pmid-2140629|pmid-3841059|pmid-1710647|pmid-11306552|pmid-10590102|pmid-14691253|pmid-15047804|pmid-16306596|pmid-15047804|pmid-16306596|pmid-9878611|pmid-11312364
Although it has been reported that the presence of NEP(NS2) protein inhibits viral RNA replication in a similar recombinant system (57), we consider this effect non-relevant to our studies, that deal with the post-transcriptional processing and export of viral mRNAs.
[ "2", "54", "55", "44", "46", "44", "46", "56", "57" ]
267
6,873
1
false
Although it has been reported that the presence of NEP protein inhibits viral RNA replication in a similar recombinant system, we consider this effect non-relevant to our studies, that deal with the post-transcriptional processing and export of viral mRNAs.
[ "NS2", "57" ]
Although it has been reported that the presence of NEP protein inhibits viral RNA replication in a similar recombinant system, we consider this effect non-relevant to our studies, that deal with the post-transcriptional processing and export of viral mRNAs.
true
true
true
true
true
1,122
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
The results presented here indicate that NS1 protein inhibits the splicing of the NS collinear transcript in a RNA-independent manner, as mutant NS1 R38A/K41A behaves as wt (Figure 6; Table 1), and preferentially blocks the nucleo-cytoplasmic export of NS1 mRNA by a mechanism dependent on NS1–RNA interaction, since mut...
[ "19", "30", "31" ]
399
6,874
0
false
The results presented here indicate that NS1 protein inhibits the splicing of the NS collinear transcript in a RNA-independent manner, as mutant NS1 R38A/K41A behaves as wt (Figure 6; Table 1), and preferentially blocks the nucleo-cytoplasmic export of NS1 mRNA by a mechanism dependent on NS1–RNA interaction, since mut...
[]
The results presented here indicate that NS1 protein inhibits the splicing of the NS collinear transcript in a RNA-independent manner, as mutant NS1 R38A/K41A behaves as wt, and preferentially blocks the nucleo-cytoplasmic export of NS1 mRNA by a mechanism dependent on NS1–RNA interaction, since mutant NS1 R38A/K41A pe...
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
Although cells in which a mutant NS R38A/K41A has been established accumulate large amounts of NS1 mRNA and very little NEP(NS2) mRNA in their cytoplasms (Figures 6 and 7), quasi-normal synthesis of NEP(NS2) protein is produced (Figures 2 and 3B).
[ "19", "30", "31" ]
247
6,875
0
false
Although cells in which a mutant NS R38A/K41A has been established accumulate large amounts of NS1 mRNA and very little NEP(NS2) mRNA in their cytoplasms (Figures 6 and 7), quasi-normal synthesis of NEP(NS2) protein is produced (Figures 2 and 3B).
[]
Although cells in which a mutant NS R38A/K41A has been established accumulate large amounts of NS1 mRNA and very little NEP(NS2) mRNA in their cytoplasms (Figures 6 and 7), quasi-normal synthesis of NEP(NS2) protein is produced (Figures 2 and 3B).
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
This apparent contradiction can be explained by the fact that, at early times in the replication and transcription of the NS R38A/K41A replicon, no NS1 protein is present in the system.
[ "19", "30", "31" ]
185
6,876
0
false
This apparent contradiction can be explained by the fact that, at early times in the replication and transcription of the NS R38A/K41A replicon, no NS1 protein is present in the system.
[]
This apparent contradiction can be explained by the fact that, at early times in the replication and transcription of the NS R38A/K41A replicon, no NS1 protein is present in the system.
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
Until NS1 protein is accumulated to the required intracellular levels, efficient splicing of the collinear transcript is to be expected, as this is the situation with a NS ΔNS1 replicon (Figure 6).
[ "19", "30", "31" ]
197
6,877
0
false
Until NS1 protein is accumulated to the required intracellular levels, efficient splicing of the collinear transcript is to be expected, as this is the situation with a NS ΔNS1 replicon (Figure 6).
[]
Until NS1 protein is accumulated to the required intracellular levels, efficient splicing of the collinear transcript is to be expected, as this is the situation with a NS ΔNS1 replicon (Figure 6).
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
Nevertheless, to account for the observed levels of NEP(NS2) synthesis it would be necessary to invoke a preferential translation of NEP(NS2) mRNA.
[ "19", "30", "31" ]
147
6,878
0
false
Nevertheless, to account for the observed levels of NEP(NS2) synthesis it would be necessary to invoke a preferential translation of NEP(NS2) mRNA.
[]
Nevertheless, to account for the observed levels of NEP(NS2) synthesis it would be necessary to invoke a preferential translation of NEP(NS2) mRNA.
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
It is conceivable that NEP(NS2) mRNA is efficiently incorporated into the cell translation machinery because it is generated at the early phase of the replicon gene expression.
[ "19", "30", "31" ]
176
6,879
0
false
It is conceivable that NEP(NS2) mRNA is efficiently incorporated into the cell translation machinery because it is generated at the early phase of the replicon gene expression.
[]
It is conceivable that NEP(NS2) mRNA is efficiently incorporated into the cell translation machinery because it is generated at the early phase of the replicon gene expression.
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
On the contrary, NS1 mRNA is produced at later times, when NS1 protein has accumulated.
[ "19", "30", "31" ]
87
6,880
0
false
On the contrary, NS1 mRNA is produced at later times, when NS1 protein has accumulated.
[]
On the contrary, NS1 mRNA is produced at later times, when NS1 protein has accumulated.
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
At that point a large fraction of the cell-derived mRNA is being used for virus-induced cap-snatching and incorporation of new mRNAs into the cell translation machinery might be affected.
[ "19", "30", "31" ]
187
6,881
0
false
At that point a large fraction of the cell-derived mRNA is being used for virus-induced cap-snatching and incorporation of new mRNAs into the cell translation machinery might be affected.
[]
At that point a large fraction of the cell-derived mRNA is being used for virus-induced cap-snatching and incorporation of new mRNAs into the cell translation machinery might be affected.
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
In addition, the level of NS1 protein present in each setting may influence the translation ability of the NEP mRNA present (19,30,31).
[ "19", "30", "31" ]
135
6,882
0
false
In addition, the level of NS1 protein present in each setting may influence the translation ability of the NEP mRNA present.
[ "19,30,31" ]
In addition, the level of NS1 protein present in each setting may influence the translation ability of the NEP mRNA present.
true
true
true
true
true
1,123
1
DISCUSSION
1
19
[ "B19", "B30", "B31" ]
17,488,845
pmid-11162793|pmid-12758165|NA|pmid-7233830|pmid-6328745|pmid-2933634|pmid-2969057|pmid-7884890|pmid-10325410|pmid-1469370|pmid-10024172|pmid-9152423|pmid-9336457|pmid-7908060|pmid-7489502|pmid-9336457|pmid-14967035|pmid-8139028|pmid-9349463|pmid-10325410|NA|pmid-14645908|pmid-10022908|pmid-9651582|pmid-10205180|pmid-9...
Thus, when NS1 is not expressed (ΔNS1 replicon), translation of NEP mRNA might be much less efficient than in those cases in which NS1 protein is abundantly expressed, as for instance in cells harbouring the wt NS replicon.
[ "19", "30", "31" ]
223
6,883
0
false
Thus, when NS1 is not expressed (ΔNS1 replicon), translation of NEP mRNA might be much less efficient than in those cases in which NS1 protein is abundantly expressed, as for instance in cells harbouring the wt NS replicon.
[]
Thus, when NS1 is not expressed, translation of NEP mRNA might be much less efficient than in those cases in which NS1 protein is abundantly expressed, as for instance in cells harbouring the wt NS replicon.
true
true
true
true
true
1,123
2
DISCUSSION
1
26
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
The effects of NS1 in the splicing and nucleo-cytoplasmic transport of mRNAs have been studied previously by several groups, including ours, but the analyses were carried out in vitro or using as targets cellular mRNAs or viral ORFs expressed as RNA polymerase II transcripts, but not true virus transcripts.
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
308
6,884
0
false
The effects of NS1 in the splicing and nucleo-cytoplasmic transport of mRNAs have been studied previously by several groups, including ours, but the analyses were carried out in vitro or using as targets cellular mRNAs or viral ORFs expressed as RNA polymerase II transcripts, but not true virus transcripts.
[]
The effects of NS1 in the splicing and nucleo-cytoplasmic transport of mRNAs have been studied previously by several groups, including ours, but the analyses were carried out in vitro or using as targets cellular mRNAs or viral ORFs expressed as RNA polymerase II transcripts, but not true virus transcripts.
true
true
true
true
true
1,124
2
DISCUSSION
1
26
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
On the basis of these studies, several models have been put forward along the years for the inhibition of pre-mRNA splicing by NS1: (i) From the results of in vitro experiments it was first proposed that the binding of NS1 to a specific motif in U6 snRNA would impede proper formation of U6-U2 and U6-U4 snRNP interactio...
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
378
6,885
0
false
On the basis of these studies, several models have been put forward along the years for the inhibition of pre-mRNA splicing by NS1: (i) From the results of in vitro experiments it was first proposed that the binding of NS1 to a specific motif in U6 snRNA would impede proper formation of U6-U2 and U6-U4 snRNP interactio...
[ "26,40" ]
On the basis of these studies, several models have been put forward along the years for the inhibition of pre-mRNA splicing by NS1: (i) From the results of in vitro experiments it was first proposed that the binding of NS1 to a specific motif in U6 snRNA would impede proper formation of U6-U2 and U6-U4 snRNP interactio...
true
true
true
true
true
1,124
2
DISCUSSION
1
40
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
Such inhibitory interaction was postulated to occur in splicing reactions on cellular pre-mRNAs but not on viral mRNAs (40).
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
124
6,886
1
false
Such inhibitory interaction was postulated to occur in splicing reactions on cellular pre-mRNAs but not on viral mRNAs.
[ "40" ]
Such inhibitory interaction was postulated to occur in splicing reactions on cellular pre-mRNAs but not on viral mRNAs.
true
true
true
true
true
1,124
2
DISCUSSION
1
41
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
This model would not explain the alterations induced by NS1 in the use of alternative splice sites (41) and is not supported by the results presented in this report, as we show that NS1 protein indeed inhibits the splicing of a true viral pre-mRNA (the NS collinear transcript) and the RNA-binding activity of NS1 is not...
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
361
6,887
1
false
This model would not explain the alterations induced by NS1 in the use of alternative splice sites and is not supported by the results presented in this report, as we show that NS1 protein indeed inhibits the splicing of a true viral pre-mRNA (the NS collinear transcript) and the RNA-binding activity of NS1 is not requ...
[ "41" ]
This model would not explain the alterations induced by NS1 in the use of alternative splice sites and is not supported by the results presented in this report, as we show that NS1 protein indeed inhibits the splicing of a true viral pre-mRNA (the NS collinear transcript) and the RNA-binding activity of NS1 is not requ...
true
true
true
true
true
1,124
2
DISCUSSION
1
33
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
(ii) After reporting that the effector domain of NS1 protein binds the 30 kDa subunit of CPSF and blocks cellular mRNA cleavage and polyadenylation (33), it was proposed that the splicing inhibition by NS1 would be an indirect consequence of the failure in 3′-end processing, as the definition of the exon in a single-ex...
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
382
6,888
1
false
(ii) After reporting that the effector domain of NS1 protein binds the 30 kDa subunit of CPSF and blocks cellular mRNA cleavage and polyadenylation, it was proposed that the splicing inhibition by NS1 would be an indirect consequence of the failure in 3′-end processing, as the definition of the exon in a single-exon co...
[ "33", "42" ]
(ii) After reporting that the effector domain of NS1 protein binds the 30 kDa subunit of CPSF and blocks cellular mRNA cleavage and polyadenylation, it was proposed that the splicing inhibition by NS1 would be an indirect consequence of the failure in 3′-end processing, as the definition of the exon in a single-exon co...
false
false
true
true
false
1,124
2
DISCUSSION
1
40
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
This model would explain the inhibition of cellular mRNA splicing and the lack of such inhibition for intron-containing viral mRNAs (40), since the 3′-polyadenylation of the latter is carried out by polymerase stuttering (58).
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
226
6,889
1
false
This model would explain the inhibition of cellular mRNA splicing and the lack of such inhibition for intron-containing viral mRNAs, since the 3′-polyadenylation of the latter is carried out by polymerase stuttering.
[ "40", "58" ]
This model would explain the inhibition of cellular mRNA splicing and the lack of such inhibition for intron-containing viral mRNAs, since the 3′-polyadenylation of the latter is carried out by polymerase stuttering.
true
true
true
true
true
1,124
2
DISCUSSION
1
26
[ "B26", "B40", "B40", "B41", "B33", "B42", "B40", "B58" ]
17,488,845
pmid-3461442|pmid-2522588|pmid-1532050|pmid-6328745|pmid-3023072|pmid-7958859|pmid-8313914|pmid-7489502|pmid-7958859|pmid-7958859|pmid-8313914|pmid-9651582|pmid-11421366|pmid-7958859|pmid-10074205
Again, our results do not support the proposed model, as NS1 protein indeed blocks the splicing of the NS collinear transcript and a NS1 mutant lacking the effector domain fully inhibits the splicing of its own mRNA (Figure 6).
[ "26", "40", "40", "41", "33", "42", "40", "58" ]
227
6,890
0
false
Again, our results do not support the proposed model, as NS1 protein indeed blocks the splicing of the NS collinear transcript and a NS1 mutant lacking the effector domain fully inhibits the splicing of its own mRNA (Figure 6).
[]
Again, our results do not support the proposed model, as NS1 protein indeed blocks the splicing of the NS collinear transcript and a NS1 mutant lacking the effector domain fully inhibits the splicing of its own mRNA.
true
true
true
true
true
1,124
3
DISCUSSION
1
35
[ "B35", "B41", "B59", "B60" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
We cannot rule out the possibility that NS1 inhibition of cellular mRNA 3′-end formation would indirectly affect the splicing of cellular pre-mRNAs, but the results presented here suggest that NS1 protein would inhibit splicing by a more general mechanism.
[ "35", "41", "59", "60" ]
256
6,891
0
false
We cannot rule out the possibility that NS1 inhibition of cellular mRNA 3′-end formation would indirectly affect the splicing of cellular pre-mRNAs, but the results presented here suggest that NS1 protein would inhibit splicing by a more general mechanism.
[]
We cannot rule out the possibility that NS1 inhibition of cellular mRNA 3′-end formation would indirectly affect the splicing of cellular pre-mRNAs, but the results presented here suggest that NS1 protein would inhibit splicing by a more general mechanism.
true
true
true
true
true
1,125
3
DISCUSSION
1
35
[ "B35", "B41", "B59", "B60" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
The interaction with NS1–BP, a human protein involved in splicing (35) is one possibility, while a de-regulation of the phosphorylation state of SR proteins is an alternative that would explain the modification in alternative splice site usage by NS1 expression (41).
[ "35", "41", "59", "60" ]
267
6,892
1
false
The interaction with NS1–BP, a human protein involved in splicing is one possibility, while a de-regulation of the phosphorylation state of SR proteins is an alternative that would explain the modification in alternative splice site usage by NS1 expression.
[ "35", "41" ]
The interaction with NS1–BP, a human protein involved in splicing is one possibility, while a de-regulation of the phosphorylation state of SR proteins is an alternative that would explain the modification in alternative splice site usage by NS1 expression.
true
true
true
true
true
1,125
3
DISCUSSION
1
59
[ "B35", "B41", "B59", "B60" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
A general inhibition of the splicing machinery would be in line with the changes in the localization of snRNPs induced by NS1 expression or virus infection (59) and could be considered as part of the viral response to inhibit the expression of cellular antiviral factors (60).
[ "35", "41", "59", "60" ]
276
6,893
1
false
A general inhibition of the splicing machinery would be in line with the changes in the localization of snRNPs induced by NS1 expression or virus infection and could be considered as part of the viral response to inhibit the expression of cellular antiviral factors.
[ "59", "60" ]
A general inhibition of the splicing machinery would be in line with the changes in the localization of snRNPs induced by NS1 expression or virus infection and could be considered as part of the viral response to inhibit the expression of cellular antiviral factors.
true
true
true
true
true
1,125
3
DISCUSSION
1
35
[ "B35", "B41", "B59", "B60" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-11421366|pmid-9696811|pmid-8313914|pmid-9049349|pmid-12667806
Whatever the mechanism involved, our data indicate that inhibition would be mediated by the N-terminal region of NS1 but would not require NS1–RNA interactions (Figure 6).
[ "35", "41", "59", "60" ]
171
6,894
0
false
Whatever the mechanism involved, our data indicate that inhibition would be mediated by the N-terminal region of NS1 but would not require NS1–RNA interactions (Figure 6).
[]
Whatever the mechanism involved, our data indicate that inhibition would be mediated by the N-terminal region of NS1 but would not require NS1–RNA interactions (Figure 6).
true
true
true
true
true
1,125
4
DISCUSSION
1
33
[ "B25", "B28", "B41", "B33", "B34", "B58", "B61", "B33", "B61", "B53", "B62" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
As indicated earlier, the nucleo-cytoplasmic transport of mRNAs is inhibited by expression of NS1 from cloned DNA (25,28,41) and a model has been proposed for such inhibition based upon the interaction of NS1 with the 30 kDa subunit of the CPSF and the subsequent inhibition of 3′-end mRNA processing (33).
[ "25", "28", "41", "33", "34", "58", "61", "33", "61", "53", "62" ]
306
6,895
1
false
As indicated earlier, the nucleo-cytoplasmic transport of mRNAs is inhibited by expression of NS1 from cloned DNA and a model has been proposed for such inhibition based upon the interaction of NS1 with the 30 kDa subunit of the CPSF and the subsequent inhibition of 3′-end mRNA processing.
[ "25,28,41", "33" ]
As indicated earlier, the nucleo-cytoplasmic transport of mRNAs is inhibited by expression of NS1 from cloned DNA and a model has been proposed for such inhibition based upon the interaction of NS1 with the 30 kDa subunit of the CPSF and the subsequent inhibition of 3′-end mRNA processing.
true
true
true
true
true
1,126
4
DISCUSSION
1
34
[ "B25", "B28", "B41", "B33", "B34", "B58", "B61", "B33", "B61", "B53", "B62" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
In addition, the interaction of NS1 with nuclear PABPII would block the export of mRNAs that could partially escape from the inhibition of 3′-end formation (34).
[ "25", "28", "41", "33", "34", "58", "61", "33", "61", "53", "62" ]
161
6,896
1
false
In addition, the interaction of NS1 with nuclear PABPII would block the export of mRNAs that could partially escape from the inhibition of 3′-end formation.
[ "34" ]
In addition, the interaction of NS1 with nuclear PABPII would block the export of mRNAs that could partially escape from the inhibition of 3′-end formation.
true
true
true
true
true
1,126
4
DISCUSSION
1
58
[ "B25", "B28", "B41", "B33", "B34", "B58", "B61", "B33", "B61", "B53", "B62" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
This model suggested that the export of true viral mRNAs would not be affected by this transport block because their poly A-tails are produced by the viral polymerase in a way independent of normal cell mRNA polyadenylation (58).
[ "25", "28", "41", "33", "34", "58", "61", "33", "61", "53", "62" ]
229
6,897
1
false
This model suggested that the export of true viral mRNAs would not be affected by this transport block because their poly A-tails are produced by the viral polymerase in a way independent of normal cell mRNA polyadenylation.
[ "58" ]
This model suggested that the export of true viral mRNAs would not be affected by this transport block because their poly A-tails are produced by the viral polymerase in a way independent of normal cell mRNA polyadenylation.
true
true
true
true
true
1,126
4
DISCUSSION
1
61
[ "B25", "B28", "B41", "B33", "B34", "B58", "B61", "B33", "B61", "B53", "B62" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
In addition, the interaction of NS1 with the TAP/p15 pathway for mRNA export has been recently described (61), leading to the block of transport of cellular mRNAs that have undergone splicing.
[ "25", "28", "41", "33", "34", "58", "61", "33", "61", "53", "62" ]
192
6,898
1
false
In addition, the interaction of NS1 with the TAP/p15 pathway for mRNA export has been recently described, leading to the block of transport of cellular mRNAs that have undergone splicing.
[ "61" ]
In addition, the interaction of NS1 with the TAP/p15 pathway for mRNA export has been recently described, leading to the block of transport of cellular mRNAs that have undergone splicing.
true
true
true
true
true
1,126
4
DISCUSSION
1
25
[ "B25", "B28", "B41", "B33", "B34", "B58", "B61", "B33", "B61", "B53", "B62" ]
17,488,845
pmid-7908060|pmid-8139028|pmid-8313914|pmid-9651582|pmid-10205180|pmid-10074205|pmid-17267598|pmid-9651582|pmid-17267598|pmid-17132145|pmid-12791298
However, the results shown in this report indicate that a preferential block of NS1 mRNA export is produced when NS1 is expressed from a NS replicon and further suggest that the RNA-binding activity of NS1 is required for the inhibition of viral mRNA export (Figure 7).
[ "25", "28", "41", "33", "34", "58", "61", "33", "61", "53", "62" ]
269
6,899
0
false
However, the results shown in this report indicate that a preferential block of NS1 mRNA export is produced when NS1 is expressed from a NS replicon and further suggest that the RNA-binding activity of NS1 is required for the inhibition of viral mRNA export (Figure 7).
[]
However, the results shown in this report indicate that a preferential block of NS1 mRNA export is produced when NS1 is expressed from a NS replicon and further suggest that the RNA-binding activity of NS1 is required for the inhibition of viral mRNA export (Figure 7).
true
true
true
true
true
1,126