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PMC1776506_F2_8620.jpg | What is the core subject represented in this visual? | Histopathologic appearance of nasal turbinate and trachea tissue (magnification ×200). A) Nasal turbinates of turkey poults exposed to human metapneumovirus (hMPV) B2, showing infiltration of inflammatory cells (hematoxylin and eosin staining; solid arrow ). B) Trachea of turkey poults exposed to hMPV B2, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). C) Nasal turbinate of sham-inoculated turkey poults. D) Trachea of sham-inoculated turkey poults. E) Nasal turbinate of turkey poults exposed to avian metapneumovirus (aMPV C), showing infiltration of inflammatory cells and multifocal loss of cilia (solid arrows). F) Trachea of turkey poults exposed to aMPV C, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). Dotted arrows indicate mucosal surface. |
PMC1776506_F2_8618.jpg | What can you see in this picture? | Histopathologic appearance of nasal turbinate and trachea tissue (magnification ×200). A) Nasal turbinates of turkey poults exposed to human metapneumovirus (hMPV) B2, showing infiltration of inflammatory cells (hematoxylin and eosin staining; solid arrow ). B) Trachea of turkey poults exposed to hMPV B2, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). C) Nasal turbinate of sham-inoculated turkey poults. D) Trachea of sham-inoculated turkey poults. E) Nasal turbinate of turkey poults exposed to avian metapneumovirus (aMPV C), showing infiltration of inflammatory cells and multifocal loss of cilia (solid arrows). F) Trachea of turkey poults exposed to aMPV C, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). Dotted arrows indicate mucosal surface. |
PMC1776506_F2_8621.jpg | What is the main focus of this visual representation? | Histopathologic appearance of nasal turbinate and trachea tissue (magnification ×200). A) Nasal turbinates of turkey poults exposed to human metapneumovirus (hMPV) B2, showing infiltration of inflammatory cells (hematoxylin and eosin staining; solid arrow ). B) Trachea of turkey poults exposed to hMPV B2, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). C) Nasal turbinate of sham-inoculated turkey poults. D) Trachea of sham-inoculated turkey poults. E) Nasal turbinate of turkey poults exposed to avian metapneumovirus (aMPV C), showing infiltration of inflammatory cells and multifocal loss of cilia (solid arrows). F) Trachea of turkey poults exposed to aMPV C, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). Dotted arrows indicate mucosal surface. |
PMC1776506_F2_8619.jpg | What is the core subject represented in this visual? | Histopathologic appearance of nasal turbinate and trachea tissue (magnification ×200). A) Nasal turbinates of turkey poults exposed to human metapneumovirus (hMPV) B2, showing infiltration of inflammatory cells (hematoxylin and eosin staining; solid arrow ). B) Trachea of turkey poults exposed to hMPV B2, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). C) Nasal turbinate of sham-inoculated turkey poults. D) Trachea of sham-inoculated turkey poults. E) Nasal turbinate of turkey poults exposed to avian metapneumovirus (aMPV C), showing infiltration of inflammatory cells and multifocal loss of cilia (solid arrows). F) Trachea of turkey poults exposed to aMPV C, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). Dotted arrows indicate mucosal surface. |
PMC1776506_F2_8623.jpg | What key item or scene is captured in this photo? | Histopathologic appearance of nasal turbinate and trachea tissue (magnification ×200). A) Nasal turbinates of turkey poults exposed to human metapneumovirus (hMPV) B2, showing infiltration of inflammatory cells (hematoxylin and eosin staining; solid arrow ). B) Trachea of turkey poults exposed to hMPV B2, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). C) Nasal turbinate of sham-inoculated turkey poults. D) Trachea of sham-inoculated turkey poults. E) Nasal turbinate of turkey poults exposed to avian metapneumovirus (aMPV C), showing infiltration of inflammatory cells and multifocal loss of cilia (solid arrows). F) Trachea of turkey poults exposed to aMPV C, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). Dotted arrows indicate mucosal surface. |
PMC1776506_F2_8622.jpg | Describe the main subject of this image. | Histopathologic appearance of nasal turbinate and trachea tissue (magnification ×200). A) Nasal turbinates of turkey poults exposed to human metapneumovirus (hMPV) B2, showing infiltration of inflammatory cells (hematoxylin and eosin staining; solid arrow ). B) Trachea of turkey poults exposed to hMPV B2, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). C) Nasal turbinate of sham-inoculated turkey poults. D) Trachea of sham-inoculated turkey poults. E) Nasal turbinate of turkey poults exposed to avian metapneumovirus (aMPV C), showing infiltration of inflammatory cells and multifocal loss of cilia (solid arrows). F) Trachea of turkey poults exposed to aMPV C, showing mild inflammation with infiltration of a few inflammatory cells in the lamina propria (solid arrow). Dotted arrows indicate mucosal surface. |
PMC1779360_F1_8625.jpg | What object or scene is depicted here? | A. Endoscopic ultrasound showing the metastatic mass in the left lobe of liver with an aspirating fine needle in it. B. Electron micrograph showing clusters of spherical shape and homogenous, medium electron dense zymogen granules in the cytoplasm (arrows). |
PMC1779360_F1_8624.jpg | What is the principal component of this image? | A. Endoscopic ultrasound showing the metastatic mass in the left lobe of liver with an aspirating fine needle in it. B. Electron micrograph showing clusters of spherical shape and homogenous, medium electron dense zymogen granules in the cytoplasm (arrows). |
PMC1779369_F1_8629.jpg | Describe the main subject of this image. | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8632.jpg | What stands out most in this visual? | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8633.jpg | What is the focal point of this photograph? | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8627.jpg | What is the focal point of this photograph? | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8630.jpg | Describe the main subject of this image. | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8631.jpg | What is the principal component of this image? | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8628.jpg | What is being portrayed in this visual content? | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F1_8626.jpg | What does this image primarily show? | CT, MRI, US and radiography of a RA patient's 2nd to 5th MCP joints. CT of the 2nd to 5th MCP joints, in (a) coronal and (b, c) axial planes. Erosions in the 3rd and 5th metacarpal heads are marked with arrows. T1-weighted magnetic resonance images of the 2nd to 5th MCP joints, in the (d, e) coronal and (f) axial planes reveal the same erosions in the 3rd and 5th metacarpal heads as marked on the CT images. US at the ulnar aspect of the 5th metacarpal head, in (g) longitudinal and (h) transversal planes. An erosion (white arrow) at the same site as detected by CT and MRI (white arrows in panels a, c, d and f) is documented in both planes. (i) Radiography reveals no erosions at the corresponding sites. CT, computed tomography; MCP, metacarpophalangeal; MRI, magneticresonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F2_8636.jpg | What is the central feature of this picture? | Radiography, CT, MRI and US of a RA patient's 2nd MCP joint. (a) Radiography in anteroposterior projection. CT in (b) coronal and (c) axial planes. T1-weighted MRIin (d) coronal and (e) axial planes. US in (f) longitudinal and (g) transversal planes. Anerosion (white arrows) at the base of the 2nd proximal phalanx isvisualized on radiography (panel a), CT (panels b and c) andultrasonography (panels f and g) in both planes. This erosion was notscored on MRI. If the corresponding area on MRI (panels d and e) isreassessed, then the reader gets the impression of the presence of anerosion, with the same configuration as on CT and radiography. CT, computed tomography; MCP, metacarpophalangeal; MRI, magnetic resonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F2_8639.jpg | What object or scene is depicted here? | Radiography, CT, MRI and US of a RA patient's 2nd MCP joint. (a) Radiography in anteroposterior projection. CT in (b) coronal and (c) axial planes. T1-weighted MRIin (d) coronal and (e) axial planes. US in (f) longitudinal and (g) transversal planes. Anerosion (white arrows) at the base of the 2nd proximal phalanx isvisualized on radiography (panel a), CT (panels b and c) andultrasonography (panels f and g) in both planes. This erosion was notscored on MRI. If the corresponding area on MRI (panels d and e) isreassessed, then the reader gets the impression of the presence of anerosion, with the same configuration as on CT and radiography. CT, computed tomography; MCP, metacarpophalangeal; MRI, magnetic resonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F2_8635.jpg | What can you see in this picture? | Radiography, CT, MRI and US of a RA patient's 2nd MCP joint. (a) Radiography in anteroposterior projection. CT in (b) coronal and (c) axial planes. T1-weighted MRIin (d) coronal and (e) axial planes. US in (f) longitudinal and (g) transversal planes. Anerosion (white arrows) at the base of the 2nd proximal phalanx isvisualized on radiography (panel a), CT (panels b and c) andultrasonography (panels f and g) in both planes. This erosion was notscored on MRI. If the corresponding area on MRI (panels d and e) isreassessed, then the reader gets the impression of the presence of anerosion, with the same configuration as on CT and radiography. CT, computed tomography; MCP, metacarpophalangeal; MRI, magnetic resonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F2_8638.jpg | What is the central feature of this picture? | Radiography, CT, MRI and US of a RA patient's 2nd MCP joint. (a) Radiography in anteroposterior projection. CT in (b) coronal and (c) axial planes. T1-weighted MRIin (d) coronal and (e) axial planes. US in (f) longitudinal and (g) transversal planes. Anerosion (white arrows) at the base of the 2nd proximal phalanx isvisualized on radiography (panel a), CT (panels b and c) andultrasonography (panels f and g) in both planes. This erosion was notscored on MRI. If the corresponding area on MRI (panels d and e) isreassessed, then the reader gets the impression of the presence of anerosion, with the same configuration as on CT and radiography. CT, computed tomography; MCP, metacarpophalangeal; MRI, magnetic resonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779369_F2_8637.jpg | What can you see in this picture? | Radiography, CT, MRI and US of a RA patient's 2nd MCP joint. (a) Radiography in anteroposterior projection. CT in (b) coronal and (c) axial planes. T1-weighted MRIin (d) coronal and (e) axial planes. US in (f) longitudinal and (g) transversal planes. Anerosion (white arrows) at the base of the 2nd proximal phalanx isvisualized on radiography (panel a), CT (panels b and c) andultrasonography (panels f and g) in both planes. This erosion was notscored on MRI. If the corresponding area on MRI (panels d and e) isreassessed, then the reader gets the impression of the presence of anerosion, with the same configuration as on CT and radiography. CT, computed tomography; MCP, metacarpophalangeal; MRI, magnetic resonance imaging; RA, rheumatoid arthritis; US, ultrasonography. |
PMC1779372_F1_8648.jpg | What is the principal component of this image? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8649.jpg | What is the central feature of this picture? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8643.jpg | Can you identify the primary element in this image? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8647.jpg | What is the core subject represented in this visual? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8645.jpg | What is the focal point of this photograph? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8646.jpg | Can you identify the primary element in this image? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8644.jpg | What is the central feature of this picture? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779372_F1_8642.jpg | What object or scene is depicted here? | Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×. |
PMC1779378_F2_8652.jpg | Describe the main subject of this image. | Parietal ramus of a normal temporal artery. Longitudinal view of the perfused lumen in colour duplex sonography; the bright area around the lumen represents the arterial wall plus the temporal fascia (right panel). With B-mode, the artery wall is still visible as two parallel bright lines (left panel). |
PMC1779378_F3_8650.jpg | What is the dominant medical problem in this image? | Halo sign in colour duplex sonography examination in a patient with giant cell arteritis. Hypoechoic area around the temporal artery trunk in longitudinal (upper panel) and transverse (lower panel) views. |
PMC1779378_F3_8651.jpg | What can you see in this picture? | Halo sign in colour duplex sonography examination in a patient with giant cell arteritis. Hypoechoic area around the temporal artery trunk in longitudinal (upper panel) and transverse (lower panel) views. |
PMC1779380_F1_8656.jpg | What object or scene is depicted here? | Electron micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a,b) Macrophages and (c,d) dendritic cells were incubated in the (a,c) absence and (b,d) presence of 200 μg/ml CII for 30 minutes and analyzed by transmission electron microscopy. The arrows show fibrils of collagen aligned parallel to the plane of the section. Magnification: (a) ×8,900; (b) ×6,610; (c) ×8,900; (d) ×21,000. Bar = 1 μm. Sections through several planes of more than 50 cells were examined for each treatment. |
PMC1779380_F1_8653.jpg | What can you see in this picture? | Electron micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a,b) Macrophages and (c,d) dendritic cells were incubated in the (a,c) absence and (b,d) presence of 200 μg/ml CII for 30 minutes and analyzed by transmission electron microscopy. The arrows show fibrils of collagen aligned parallel to the plane of the section. Magnification: (a) ×8,900; (b) ×6,610; (c) ×8,900; (d) ×21,000. Bar = 1 μm. Sections through several planes of more than 50 cells were examined for each treatment. |
PMC1779380_F1_8654.jpg | What is the main focus of this visual representation? | Electron micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a,b) Macrophages and (c,d) dendritic cells were incubated in the (a,c) absence and (b,d) presence of 200 μg/ml CII for 30 minutes and analyzed by transmission electron microscopy. The arrows show fibrils of collagen aligned parallel to the plane of the section. Magnification: (a) ×8,900; (b) ×6,610; (c) ×8,900; (d) ×21,000. Bar = 1 μm. Sections through several planes of more than 50 cells were examined for each treatment. |
PMC1779380_F2_8659.jpg | What is the dominant medical problem in this image? | Electron micrographs of the effect of inhibitors of uptake on type II collagen (CII) internalization by macrophages. Macrophages were pulsed with 200 μg/ml CII for 30 minutes in the presence of (a) 10.0 μM cytochalasin D, (b) 1.0 mM amiloride, (c) 5.0 μM monodansylcadaverine (MDC) or (d) 0.4 μg/ml filipin and analyzed by electron microscopy. Magnification: (a) ×6,610; (b) ×52,000; (c) ×21,000; (d) ×73,000. Bar = (a,c) 1 μm or (b,d) 200 nm. Black arrows show fibrils of collagen aligned parallel to the plane of the section; the white arrow shows an unwinding collagen fibril inside the cell. Sections through several planes of more than 50 cells were examined for each treatment. |
PMC1779380_F2_8657.jpg | What's the most prominent thing you notice in this picture? | Electron micrographs of the effect of inhibitors of uptake on type II collagen (CII) internalization by macrophages. Macrophages were pulsed with 200 μg/ml CII for 30 minutes in the presence of (a) 10.0 μM cytochalasin D, (b) 1.0 mM amiloride, (c) 5.0 μM monodansylcadaverine (MDC) or (d) 0.4 μg/ml filipin and analyzed by electron microscopy. Magnification: (a) ×6,610; (b) ×52,000; (c) ×21,000; (d) ×73,000. Bar = (a,c) 1 μm or (b,d) 200 nm. Black arrows show fibrils of collagen aligned parallel to the plane of the section; the white arrow shows an unwinding collagen fibril inside the cell. Sections through several planes of more than 50 cells were examined for each treatment. |
PMC1779380_F3_8662.jpg | What can you see in this picture? | Confocal micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a-c) Macrophages and (d-f) dendritic cells were incubated in the (a,b,d,e) presence or (c,f) absence of 200 μg/ml CII for 30 minutes, stained for CII expression and analyzed by confocal microscopy. Magnification ×630, and bars denote (a) 6.63 μm, (b) 8.09 μm, (c) 5.0 μm, (d) 4.27 μm, (e) 4.64 μm and (f) 4.0 μm. More than 50 cells were examined for each treatment. |
PMC1779380_F3_8666.jpg | What's the most prominent thing you notice in this picture? | Confocal micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a-c) Macrophages and (d-f) dendritic cells were incubated in the (a,b,d,e) presence or (c,f) absence of 200 μg/ml CII for 30 minutes, stained for CII expression and analyzed by confocal microscopy. Magnification ×630, and bars denote (a) 6.63 μm, (b) 8.09 μm, (c) 5.0 μm, (d) 4.27 μm, (e) 4.64 μm and (f) 4.0 μm. More than 50 cells were examined for each treatment. |
PMC1779380_F3_8664.jpg | What can you see in this picture? | Confocal micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a-c) Macrophages and (d-f) dendritic cells were incubated in the (a,b,d,e) presence or (c,f) absence of 200 μg/ml CII for 30 minutes, stained for CII expression and analyzed by confocal microscopy. Magnification ×630, and bars denote (a) 6.63 μm, (b) 8.09 μm, (c) 5.0 μm, (d) 4.27 μm, (e) 4.64 μm and (f) 4.0 μm. More than 50 cells were examined for each treatment. |
PMC1779380_F3_8661.jpg | What is the dominant medical problem in this image? | Confocal micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a-c) Macrophages and (d-f) dendritic cells were incubated in the (a,b,d,e) presence or (c,f) absence of 200 μg/ml CII for 30 minutes, stained for CII expression and analyzed by confocal microscopy. Magnification ×630, and bars denote (a) 6.63 μm, (b) 8.09 μm, (c) 5.0 μm, (d) 4.27 μm, (e) 4.64 μm and (f) 4.0 μm. More than 50 cells were examined for each treatment. |
PMC1779385_F7_8673.jpg | What is being portrayed in this visual content? | BMP-2 and BMP-7 co-localization with AP in bursa tissue with and without C2C12 cells. Indirect immunofluorescence staining of the topical distribution of (a) bone morphogenetic protein (BMP)-2 (green) and (b) BMP-7 (red) and their local effects on C2C12 cells. The images display objective magnifications (×5) of subsequent frozen sections. (c) A section without cultured C2C12 cells stained directly for alkaline phosphatase (AP). (d) A section seeded with C2C12 cells and cultured for 5 days before staining for AP. Note the co-distribution of AP, both the bursa-derived intrinsic enzyme and the C2C12-derived enzyme, with the BMP deposits within the tissue. |
PMC1779385_F7_8671.jpg | What does this image primarily show? | BMP-2 and BMP-7 co-localization with AP in bursa tissue with and without C2C12 cells. Indirect immunofluorescence staining of the topical distribution of (a) bone morphogenetic protein (BMP)-2 (green) and (b) BMP-7 (red) and their local effects on C2C12 cells. The images display objective magnifications (×5) of subsequent frozen sections. (c) A section without cultured C2C12 cells stained directly for alkaline phosphatase (AP). (d) A section seeded with C2C12 cells and cultured for 5 days before staining for AP. Note the co-distribution of AP, both the bursa-derived intrinsic enzyme and the C2C12-derived enzyme, with the BMP deposits within the tissue. |
PMC1779385_F7_8672.jpg | What is the core subject represented in this visual? | BMP-2 and BMP-7 co-localization with AP in bursa tissue with and without C2C12 cells. Indirect immunofluorescence staining of the topical distribution of (a) bone morphogenetic protein (BMP)-2 (green) and (b) BMP-7 (red) and their local effects on C2C12 cells. The images display objective magnifications (×5) of subsequent frozen sections. (c) A section without cultured C2C12 cells stained directly for alkaline phosphatase (AP). (d) A section seeded with C2C12 cells and cultured for 5 days before staining for AP. Note the co-distribution of AP, both the bursa-derived intrinsic enzyme and the C2C12-derived enzyme, with the BMP deposits within the tissue. |
PMC1779385_F7_8674.jpg | What is the main focus of this visual representation? | BMP-2 and BMP-7 co-localization with AP in bursa tissue with and without C2C12 cells. Indirect immunofluorescence staining of the topical distribution of (a) bone morphogenetic protein (BMP)-2 (green) and (b) BMP-7 (red) and their local effects on C2C12 cells. The images display objective magnifications (×5) of subsequent frozen sections. (c) A section without cultured C2C12 cells stained directly for alkaline phosphatase (AP). (d) A section seeded with C2C12 cells and cultured for 5 days before staining for AP. Note the co-distribution of AP, both the bursa-derived intrinsic enzyme and the C2C12-derived enzyme, with the BMP deposits within the tissue. |
PMC1779388_F4_8669.jpg | What is the dominant medical problem in this image? | Histological assessment of arthritic synovium sections from spontaneous polychondritis and type II collagen-induced polychondritis. Sections obtained from several arthritic rear paws were stained either with (a), (b) hematoxylin and eosin to determine the general cellular infiltration or with (c), (d) Toludine Blue to determine the mast cell infiltration. The cellular infiltration during (a), (c) spontaneous polychondritis is compared with (b), (d) type II collagen-induced polychondritis. All sections are displayed at 10 ×. |
PMC1779388_F4_8668.jpg | What is the dominant medical problem in this image? | Histological assessment of arthritic synovium sections from spontaneous polychondritis and type II collagen-induced polychondritis. Sections obtained from several arthritic rear paws were stained either with (a), (b) hematoxylin and eosin to determine the general cellular infiltration or with (c), (d) Toludine Blue to determine the mast cell infiltration. The cellular infiltration during (a), (c) spontaneous polychondritis is compared with (b), (d) type II collagen-induced polychondritis. All sections are displayed at 10 ×. |
PMC1779408_F1_8678.jpg | What can you see in this picture? | Therapeutic effects of hepatocyte growth factor (HGF) on the progression of lupus nephritis. (a) Protective effect of HGF against the development of nephritic syndrome, as determined by changes in urine protein excretion levels. Open circles, HGF-treated graft-versus-host disease (GVHD; n = 10); closed circles, untreated GVHD (n = 10). Data represent mean ± standard error (n = 10). *p < 0.05; **p < 0.01 compared to untreated GVHD at the same time point. (b) Histopathology of kidney tissue from untreated and HGF-treated GVHD mice. Mononuclear cell infiltration into the perivascular area of the kidney was observed at 3 weeks, and glomerulonephritis, as shown by glomerular enlargement, increased glomerular lobularity, mesangial hypercellularity, and membrane thickening was observed at 12 weeks after GVHD induction. HGF treatment significantly inhibited these pathological changes. Original magnification ×200. |
PMC1779408_F1_8679.jpg | What can you see in this picture? | Therapeutic effects of hepatocyte growth factor (HGF) on the progression of lupus nephritis. (a) Protective effect of HGF against the development of nephritic syndrome, as determined by changes in urine protein excretion levels. Open circles, HGF-treated graft-versus-host disease (GVHD; n = 10); closed circles, untreated GVHD (n = 10). Data represent mean ± standard error (n = 10). *p < 0.05; **p < 0.01 compared to untreated GVHD at the same time point. (b) Histopathology of kidney tissue from untreated and HGF-treated GVHD mice. Mononuclear cell infiltration into the perivascular area of the kidney was observed at 3 weeks, and glomerulonephritis, as shown by glomerular enlargement, increased glomerular lobularity, mesangial hypercellularity, and membrane thickening was observed at 12 weeks after GVHD induction. HGF treatment significantly inhibited these pathological changes. Original magnification ×200. |
PMC1779408_F1_8675.jpg | What's the most prominent thing you notice in this picture? | Therapeutic effects of hepatocyte growth factor (HGF) on the progression of lupus nephritis. (a) Protective effect of HGF against the development of nephritic syndrome, as determined by changes in urine protein excretion levels. Open circles, HGF-treated graft-versus-host disease (GVHD; n = 10); closed circles, untreated GVHD (n = 10). Data represent mean ± standard error (n = 10). *p < 0.05; **p < 0.01 compared to untreated GVHD at the same time point. (b) Histopathology of kidney tissue from untreated and HGF-treated GVHD mice. Mononuclear cell infiltration into the perivascular area of the kidney was observed at 3 weeks, and glomerulonephritis, as shown by glomerular enlargement, increased glomerular lobularity, mesangial hypercellularity, and membrane thickening was observed at 12 weeks after GVHD induction. HGF treatment significantly inhibited these pathological changes. Original magnification ×200. |
PMC1779408_F1_8676.jpg | What is the focal point of this photograph? | Therapeutic effects of hepatocyte growth factor (HGF) on the progression of lupus nephritis. (a) Protective effect of HGF against the development of nephritic syndrome, as determined by changes in urine protein excretion levels. Open circles, HGF-treated graft-versus-host disease (GVHD; n = 10); closed circles, untreated GVHD (n = 10). Data represent mean ± standard error (n = 10). *p < 0.05; **p < 0.01 compared to untreated GVHD at the same time point. (b) Histopathology of kidney tissue from untreated and HGF-treated GVHD mice. Mononuclear cell infiltration into the perivascular area of the kidney was observed at 3 weeks, and glomerulonephritis, as shown by glomerular enlargement, increased glomerular lobularity, mesangial hypercellularity, and membrane thickening was observed at 12 weeks after GVHD induction. HGF treatment significantly inhibited these pathological changes. Original magnification ×200. |
PMC1779410_F2_8682.jpg | Can you identify the primary element in this image? | Imaging of adherent leukocytes in vivo. The figure shows a representative fluorescence microscope image (original magnification × 306) of a postcapillary venule showing an increased number of cells adhering to the wall of the postcapillary venule in a control animal that received the anti-collagen II (CII) antibodies and a pre-treatment with NaCl compared to an antileukoproteinase-treated mouse at 24 hours after anti-CII mAb transfer. |
PMC1779410_F2_8681.jpg | What is shown in this image? | Imaging of adherent leukocytes in vivo. The figure shows a representative fluorescence microscope image (original magnification × 306) of a postcapillary venule showing an increased number of cells adhering to the wall of the postcapillary venule in a control animal that received the anti-collagen II (CII) antibodies and a pre-treatment with NaCl compared to an antileukoproteinase-treated mouse at 24 hours after anti-CII mAb transfer. |
PMC1779418_F4_8683.jpg | What is the central feature of this picture? | Histological analysis of synovial tissue recovered from the rabbit knees. Twenty-four hours after antigen-induced arthritis (AIA) induction, rabbits received either Ad.hIL-10, Ad.vIL-10, Ad.mut.hIL-10 or Ad.eGFP. On day 7 after the adenoviral delivery, the rabbits were sacrificed, and synovial tissue harvested, fixed, sectioned, and stained with hematoxylin and eosin. (a) Synovium from a naïve control rabbit knee. (b) Synovium from an AIA rabbit knee that received Ad.eGFP as an inflamed control. (c) Synovium from an AIA rabbit knee that was treated with Ad.hIL-10. (d) Synovium from an AIA rabbit knee that was treated with Ad.vIL-10. (e) Synovium from an AIA rabbit knee that was treated with Ad.mut.hIL-10. hIL-10, human IL-10; mut.hIL-10, mutant human IL-10; vIL-10, viral IL-10; eGFP, enhanced green fluorescent protein. |
PMC1779418_F4_8685.jpg | What's the most prominent thing you notice in this picture? | Histological analysis of synovial tissue recovered from the rabbit knees. Twenty-four hours after antigen-induced arthritis (AIA) induction, rabbits received either Ad.hIL-10, Ad.vIL-10, Ad.mut.hIL-10 or Ad.eGFP. On day 7 after the adenoviral delivery, the rabbits were sacrificed, and synovial tissue harvested, fixed, sectioned, and stained with hematoxylin and eosin. (a) Synovium from a naïve control rabbit knee. (b) Synovium from an AIA rabbit knee that received Ad.eGFP as an inflamed control. (c) Synovium from an AIA rabbit knee that was treated with Ad.hIL-10. (d) Synovium from an AIA rabbit knee that was treated with Ad.vIL-10. (e) Synovium from an AIA rabbit knee that was treated with Ad.mut.hIL-10. hIL-10, human IL-10; mut.hIL-10, mutant human IL-10; vIL-10, viral IL-10; eGFP, enhanced green fluorescent protein. |
PMC1779418_F4_8686.jpg | What is the central feature of this picture? | Histological analysis of synovial tissue recovered from the rabbit knees. Twenty-four hours after antigen-induced arthritis (AIA) induction, rabbits received either Ad.hIL-10, Ad.vIL-10, Ad.mut.hIL-10 or Ad.eGFP. On day 7 after the adenoviral delivery, the rabbits were sacrificed, and synovial tissue harvested, fixed, sectioned, and stained with hematoxylin and eosin. (a) Synovium from a naïve control rabbit knee. (b) Synovium from an AIA rabbit knee that received Ad.eGFP as an inflamed control. (c) Synovium from an AIA rabbit knee that was treated with Ad.hIL-10. (d) Synovium from an AIA rabbit knee that was treated with Ad.vIL-10. (e) Synovium from an AIA rabbit knee that was treated with Ad.mut.hIL-10. hIL-10, human IL-10; mut.hIL-10, mutant human IL-10; vIL-10, viral IL-10; eGFP, enhanced green fluorescent protein. |
PMC1779418_F4_8684.jpg | What does this image primarily show? | Histological analysis of synovial tissue recovered from the rabbit knees. Twenty-four hours after antigen-induced arthritis (AIA) induction, rabbits received either Ad.hIL-10, Ad.vIL-10, Ad.mut.hIL-10 or Ad.eGFP. On day 7 after the adenoviral delivery, the rabbits were sacrificed, and synovial tissue harvested, fixed, sectioned, and stained with hematoxylin and eosin. (a) Synovium from a naïve control rabbit knee. (b) Synovium from an AIA rabbit knee that received Ad.eGFP as an inflamed control. (c) Synovium from an AIA rabbit knee that was treated with Ad.hIL-10. (d) Synovium from an AIA rabbit knee that was treated with Ad.vIL-10. (e) Synovium from an AIA rabbit knee that was treated with Ad.mut.hIL-10. hIL-10, human IL-10; mut.hIL-10, mutant human IL-10; vIL-10, viral IL-10; eGFP, enhanced green fluorescent protein. |
PMC1779434_F1_8694.jpg | What does this image primarily show? | Macroscopic and microscopic assessment of zygapophyseal joints. (a) Macroscopic picture of a zygapophyseal joint from a patient with ankylosing spondylitis (AS). (b) Hematoxylin and eosin staining of a zygapophyseal joint from an AS patient: weakly eosinophilic fluid accumulation in the bone marrow interstitium (red arrows) neighboring dense interstitial infiltrates of mononuclear cells (yellow arrows). The black arrow indicates fat vacuoles. (c) Immunohistochemical analysis of CD3+ T cells in the zygapophyseal joint of an AS patient: dense formations of CD3+ T cells (red stain, yellow arrow) neighboring bone marrow edema (red arrow). (d) Immunohistochemical analysis of CD3+ T cells in a zygapophyseal joint of a non-AS control with only few loosely distributed CD3+ T cells in the bone marrow without edema. |
PMC1779434_F1_8692.jpg | What object or scene is depicted here? | Macroscopic and microscopic assessment of zygapophyseal joints. (a) Macroscopic picture of a zygapophyseal joint from a patient with ankylosing spondylitis (AS). (b) Hematoxylin and eosin staining of a zygapophyseal joint from an AS patient: weakly eosinophilic fluid accumulation in the bone marrow interstitium (red arrows) neighboring dense interstitial infiltrates of mononuclear cells (yellow arrows). The black arrow indicates fat vacuoles. (c) Immunohistochemical analysis of CD3+ T cells in the zygapophyseal joint of an AS patient: dense formations of CD3+ T cells (red stain, yellow arrow) neighboring bone marrow edema (red arrow). (d) Immunohistochemical analysis of CD3+ T cells in a zygapophyseal joint of a non-AS control with only few loosely distributed CD3+ T cells in the bone marrow without edema. |
PMC1779434_F1_8691.jpg | What can you see in this picture? | Macroscopic and microscopic assessment of zygapophyseal joints. (a) Macroscopic picture of a zygapophyseal joint from a patient with ankylosing spondylitis (AS). (b) Hematoxylin and eosin staining of a zygapophyseal joint from an AS patient: weakly eosinophilic fluid accumulation in the bone marrow interstitium (red arrows) neighboring dense interstitial infiltrates of mononuclear cells (yellow arrows). The black arrow indicates fat vacuoles. (c) Immunohistochemical analysis of CD3+ T cells in the zygapophyseal joint of an AS patient: dense formations of CD3+ T cells (red stain, yellow arrow) neighboring bone marrow edema (red arrow). (d) Immunohistochemical analysis of CD3+ T cells in a zygapophyseal joint of a non-AS control with only few loosely distributed CD3+ T cells in the bone marrow without edema. |
PMC1779434_F2_8690.jpg | Describe the main subject of this image. | Positive correlation of bone marrow edema in histopathological assessment and magnetic resonance imaging. (a) Magnetic resonance imaging (T2 sequence) of a zygapophyseal joint with bone marrow edema (cyan arrow) (asterisk, vertebral body; open square, epidural space; plus signs, superior and inferior articular processes of the zygapophyseal joint; hash sign, processus spinosus). (b) Hematoxylin and eosin staining, revealing dense infiltrations of mononuclear cells (yellow arrow) and interstitial bone marrow edema (red arrow). (c) Immunohistochemical analysis of CD3+ T cells, revealing mononuclear cell aggregates in the same zygapophyseal joint (yellow arrow). |
PMC1779434_F2_8688.jpg | What key item or scene is captured in this photo? | Positive correlation of bone marrow edema in histopathological assessment and magnetic resonance imaging. (a) Magnetic resonance imaging (T2 sequence) of a zygapophyseal joint with bone marrow edema (cyan arrow) (asterisk, vertebral body; open square, epidural space; plus signs, superior and inferior articular processes of the zygapophyseal joint; hash sign, processus spinosus). (b) Hematoxylin and eosin staining, revealing dense infiltrations of mononuclear cells (yellow arrow) and interstitial bone marrow edema (red arrow). (c) Immunohistochemical analysis of CD3+ T cells, revealing mononuclear cell aggregates in the same zygapophyseal joint (yellow arrow). |
PMC1779434_F2_8689.jpg | What can you see in this picture? | Positive correlation of bone marrow edema in histopathological assessment and magnetic resonance imaging. (a) Magnetic resonance imaging (T2 sequence) of a zygapophyseal joint with bone marrow edema (cyan arrow) (asterisk, vertebral body; open square, epidural space; plus signs, superior and inferior articular processes of the zygapophyseal joint; hash sign, processus spinosus). (b) Hematoxylin and eosin staining, revealing dense infiltrations of mononuclear cells (yellow arrow) and interstitial bone marrow edema (red arrow). (c) Immunohistochemical analysis of CD3+ T cells, revealing mononuclear cell aggregates in the same zygapophyseal joint (yellow arrow). |
PMC1779434_F3_8695.jpg | What is being portrayed in this visual content? | Negative correlation of bone marrow edema in histopathological assessment and magnetic resonance imaging. (a) Magnetic resonance imaging of zygapophyseal joints (cyan arrows) without detectable bone marrow edema (asterisk, vertebral body; open square, epidural space; plus signs, superior and inferior articular processes of the zygapophyseal joint; hash sign, processus spinosus). (b) Hematoxylin and eosin staining of the zygapophyseal joint, revealing numerous mononuclear cells in the bone marrow (yellow arrow) and a relatively small amount of edema (20%) in the bone marrow (red arrow). (c) Immunohistochemical analysis of CD3+ T cells (red arrow), showing dense infiltrates of CD3+ T cells (yellow arrow). |
PMC1779434_F3_8696.jpg | What is shown in this image? | Negative correlation of bone marrow edema in histopathological assessment and magnetic resonance imaging. (a) Magnetic resonance imaging of zygapophyseal joints (cyan arrows) without detectable bone marrow edema (asterisk, vertebral body; open square, epidural space; plus signs, superior and inferior articular processes of the zygapophyseal joint; hash sign, processus spinosus). (b) Hematoxylin and eosin staining of the zygapophyseal joint, revealing numerous mononuclear cells in the bone marrow (yellow arrow) and a relatively small amount of edema (20%) in the bone marrow (red arrow). (c) Immunohistochemical analysis of CD3+ T cells (red arrow), showing dense infiltrates of CD3+ T cells (yellow arrow). |
PMC1779434_F3_8697.jpg | What is the main focus of this visual representation? | Negative correlation of bone marrow edema in histopathological assessment and magnetic resonance imaging. (a) Magnetic resonance imaging of zygapophyseal joints (cyan arrows) without detectable bone marrow edema (asterisk, vertebral body; open square, epidural space; plus signs, superior and inferior articular processes of the zygapophyseal joint; hash sign, processus spinosus). (b) Hematoxylin and eosin staining of the zygapophyseal joint, revealing numerous mononuclear cells in the bone marrow (yellow arrow) and a relatively small amount of edema (20%) in the bone marrow (red arrow). (c) Immunohistochemical analysis of CD3+ T cells (red arrow), showing dense infiltrates of CD3+ T cells (yellow arrow). |
PMC1779463_F1_8698.jpg | What is the main focus of this visual representation? | Unsupervised hierarchical clustering of paired core biopsy and surgical tumor tissue samples (Spearman correlation, average linkage). Red color indicates high expression levels (low ΔCt) and green vice versa. Patients 6, 11 and 20 had two core biopsies taken that were analyzed separately. In four cases the paired gene expression profiles did not cluster together close to each other. |
PMC1779486_F7_8700.jpg | What is the central feature of this picture? | Images of pauci cellular fibrotic and cellular stroma sections from selected patients. Images were taken at 4× and 10× magnification. |
PMC1779486_F7_8703.jpg | What's the most prominent thing you notice in this picture? | Images of pauci cellular fibrotic and cellular stroma sections from selected patients. Images were taken at 4× and 10× magnification. |
PMC1779486_F7_8702.jpg | What does this image primarily show? | Images of pauci cellular fibrotic and cellular stroma sections from selected patients. Images were taken at 4× and 10× magnification. |
PMC1779486_F7_8701.jpg | What's the most prominent thing you notice in this picture? | Images of pauci cellular fibrotic and cellular stroma sections from selected patients. Images were taken at 4× and 10× magnification. |
PMC1779490_F1_8706.jpg | What is the central feature of this picture? | Disseminated apoptotic and nonapoptotic disseminated tumor cells. (a) Nonapoptotic disseminated tumor cell (DTC) (detected by the APAAP kit method). (b) Apoptotic DTC identified by M30 staining and morphological assessment (for example, membrane blebbing, nuclear shrinkage) (APAAP kit detection method). (c) Apoptotic DTC identified by morphological assessment (nuclear shrinkage, cytokeratin inclusions) (DTC detected by a fluorescein-5-isothiocyanate-labeled anticytokeratin antibody). (d) Apoptotic cell body without detectable amounts of chromatin. |
PMC1779490_F1_8705.jpg | Can you identify the primary element in this image? | Disseminated apoptotic and nonapoptotic disseminated tumor cells. (a) Nonapoptotic disseminated tumor cell (DTC) (detected by the APAAP kit method). (b) Apoptotic DTC identified by M30 staining and morphological assessment (for example, membrane blebbing, nuclear shrinkage) (APAAP kit detection method). (c) Apoptotic DTC identified by morphological assessment (nuclear shrinkage, cytokeratin inclusions) (DTC detected by a fluorescein-5-isothiocyanate-labeled anticytokeratin antibody). (d) Apoptotic cell body without detectable amounts of chromatin. |
PMC1779490_F1_8707.jpg | What key item or scene is captured in this photo? | Disseminated apoptotic and nonapoptotic disseminated tumor cells. (a) Nonapoptotic disseminated tumor cell (DTC) (detected by the APAAP kit method). (b) Apoptotic DTC identified by M30 staining and morphological assessment (for example, membrane blebbing, nuclear shrinkage) (APAAP kit detection method). (c) Apoptotic DTC identified by morphological assessment (nuclear shrinkage, cytokeratin inclusions) (DTC detected by a fluorescein-5-isothiocyanate-labeled anticytokeratin antibody). (d) Apoptotic cell body without detectable amounts of chromatin. |
PMC1779492_F4_8708.jpg | What is the principal component of this image? | Magnetic resonance mammography 2 years after therapies for invasive ductal carcinoma. This shows two lesions (arrows) (Breast Imaging Reporting and Data System IV, V), reported by biopsy as malignant. |
PMC1779495_F1_8713.jpg | What is the dominant medical problem in this image? | TIMP-3 protein expression in breast cancer and normal epithelium. The micrographs show tissue inhibitor of metalloproteinases (TIMP)-3 in the cytoplasm of a lobular carcinoma (a), both the cancer cells and peritumoral stroma of a ductal carcinoma (b), in situ carcinoma (c) and normal epithelium (d). Envision/horseradish peroxidase staining; original magnification ×200. |
PMC1779495_F1_8711.jpg | What is the main focus of this visual representation? | TIMP-3 protein expression in breast cancer and normal epithelium. The micrographs show tissue inhibitor of metalloproteinases (TIMP)-3 in the cytoplasm of a lobular carcinoma (a), both the cancer cells and peritumoral stroma of a ductal carcinoma (b), in situ carcinoma (c) and normal epithelium (d). Envision/horseradish peroxidase staining; original magnification ×200. |
PMC1779495_F1_8714.jpg | What key item or scene is captured in this photo? | TIMP-3 protein expression in breast cancer and normal epithelium. The micrographs show tissue inhibitor of metalloproteinases (TIMP)-3 in the cytoplasm of a lobular carcinoma (a), both the cancer cells and peritumoral stroma of a ductal carcinoma (b), in situ carcinoma (c) and normal epithelium (d). Envision/horseradish peroxidase staining; original magnification ×200. |
PMC1779495_F1_8712.jpg | What is the focal point of this photograph? | TIMP-3 protein expression in breast cancer and normal epithelium. The micrographs show tissue inhibitor of metalloproteinases (TIMP)-3 in the cytoplasm of a lobular carcinoma (a), both the cancer cells and peritumoral stroma of a ductal carcinoma (b), in situ carcinoma (c) and normal epithelium (d). Envision/horseradish peroxidase staining; original magnification ×200. |
PMC1779504_F1_8717.jpg | What stands out most in this visual? | Examples of MRI scans and whole-tumour sections stained
with haematoxylin and erythrosine from 2 different
leiomyosarcomas; (a) a subcutaneous tumour of the thigh with a
pushing growth pattern on MRI, but microscopic infiltration on
histopathology, (b) an intramuscular tumour of the thigh with
focally infiltrative growth pattern on both MRI and
histopathology. |
PMC1779504_F1_8715.jpg | What is shown in this image? | Examples of MRI scans and whole-tumour sections stained
with haematoxylin and erythrosine from 2 different
leiomyosarcomas; (a) a subcutaneous tumour of the thigh with a
pushing growth pattern on MRI, but microscopic infiltration on
histopathology, (b) an intramuscular tumour of the thigh with
focally infiltrative growth pattern on both MRI and
histopathology. |
PMC1779504_F1_8716.jpg | What is the principal component of this image? | Examples of MRI scans and whole-tumour sections stained
with haematoxylin and erythrosine from 2 different
leiomyosarcomas; (a) a subcutaneous tumour of the thigh with a
pushing growth pattern on MRI, but microscopic infiltration on
histopathology, (b) an intramuscular tumour of the thigh with
focally infiltrative growth pattern on both MRI and
histopathology. |
PMC1779504_F1_8718.jpg | Describe the main subject of this image. | Examples of MRI scans and whole-tumour sections stained
with haematoxylin and erythrosine from 2 different
leiomyosarcomas; (a) a subcutaneous tumour of the thigh with a
pushing growth pattern on MRI, but microscopic infiltration on
histopathology, (b) an intramuscular tumour of the thigh with
focally infiltrative growth pattern on both MRI and
histopathology. |
PMC1779506_F1_8719.jpg | What is shown in this image? | CT scan of the head shows a large vascular soft tissue mass involving the meninges and invasion of right parietal bone. |
PMC1779607_F1_8720.jpg | What stands out most in this visual? | Photograph of embryos recovered from control and
LPS-treated nonsuperovulated pregnant mice during different stages
of preimplantation period of pregnancy. Panels (a), (b), (c), and
(d) show embryos recovered from control animals on days 1.5,
2.5, 3.5, and 4.375 of pregnancy, respectively. Panels (e),
(f), (g), and (h) show embryos recovered from the animals treated
with the “minimum dose” of LPS on days 1.5, 2.5, 3.5, and
4.375 of pregnancy, respectively, X100. |
PMC1779607_F2_8721.jpg | What can you see in this picture? | Photograph of embryos recovered from control and
LPS-treated superovulated pregnant mice during different stages of
preimplantation period of pregnancy. Panels (a), (b), (c), and (d)
show embryos recovered from control animals on days 1.5, 2.5,
3.5, and 4.375 of pregnancy, respectively. Panels (e), (f),
(g), and (h) show embryos recovered from the animals treated with
the “minimum dose” of LPS on days 1.5, 2.5, 3.5, and
4.375 of pregnancy, respectively, X100. |
PMC1779615_F1_8723.jpg | What is the focal point of this photograph? | (a) Transvaginal ultrasound image, shortly
after initial evaluation, of persistent bilateral ovarian masses
adjacent to uterus (Ut). (b) Ultrasound image of left
ovarian cystic structure after patient presented with symptoms of
acute pelvic inflammatory disease. |
PMC1779623_pone-0000187-g005_8730.jpg | What is the core subject represented in this visual? | Directed expression of p[UAS-RNAi-HMGCR] specifically in the corpus allatum mimics the hmgcr mutation, by disrupting the sexual dimorphism.For all panels (a–g): immunostaining using a primary antibody raised against the human form of the HMGCR, revealed with a secondary antibody labeled with Cy3 (red). a–d) Targeting UAS-RNAi-HMGCR specifically in the ca yields to the lack of the HMGCR protein, and leads to the disruption of the sexual dimorphism. This effect is completely reversible. In 2 days old DI-3/UAS-RNAi-HMGCR flies (b), HMGCR protein is not detectable and the number of start/stop is identical between males and females compared to controls (a) (2 days old UAS-RNAi-HMGCR/CS flies). Interestingly, in 5 days old flies, both DI-3/UAS-RNAi-HMGCR (d) and UAS-RNAi-HMGCR/CS (c) the HMGCR is detectable and the start/stop number is sexually dimorphic. This is in agreement with the temporal expression pattern driven by the DI-3. Additionally, this result strongly suggests the reversibility of the RNAi effect. N.B: results from b) and d) come from the same flies, recorded at 2 and 5 days old respectively. e, f) Expressing the UAS-RNAi-HMGCR in the ca using the DI-11 line (DI-11/UAS-RNAi-HMGCR) also leads to the lack of the HMGCR product (f) and disrupts the sexual dimorphism compared to controls flies (e). g) The HMGCR is expressed both in the cardia and the ca (left panel) in control flies (2 days old UAS-RNAi-HMGCR/CS flies), whereas in 2 days old (DI-3/UAS-RNAi-HMGCR) flies, the HMGCR is detected only in the cardia (right panel). This last staining serves as a positive control, to demonstrate that the DI-3 drives the UAS-RNAi-HMGCR only in the ca. h) Expressing the UAS-RNAi-HMGCR in the ca under the control of DI-11 leads to a strong lethality when flies are reared at 24°C. Moreover, the only few females that survived are dwarf (left: dwarf female DI-11/UAS-RNAi-HMGCR, middle: female control UAS-RNAi-HMGCR/CS] and right: female CS). Scale bar = 25 µm. |
PMC1779623_pone-0000187-g005_8734.jpg | What is shown in this image? | Directed expression of p[UAS-RNAi-HMGCR] specifically in the corpus allatum mimics the hmgcr mutation, by disrupting the sexual dimorphism.For all panels (a–g): immunostaining using a primary antibody raised against the human form of the HMGCR, revealed with a secondary antibody labeled with Cy3 (red). a–d) Targeting UAS-RNAi-HMGCR specifically in the ca yields to the lack of the HMGCR protein, and leads to the disruption of the sexual dimorphism. This effect is completely reversible. In 2 days old DI-3/UAS-RNAi-HMGCR flies (b), HMGCR protein is not detectable and the number of start/stop is identical between males and females compared to controls (a) (2 days old UAS-RNAi-HMGCR/CS flies). Interestingly, in 5 days old flies, both DI-3/UAS-RNAi-HMGCR (d) and UAS-RNAi-HMGCR/CS (c) the HMGCR is detectable and the start/stop number is sexually dimorphic. This is in agreement with the temporal expression pattern driven by the DI-3. Additionally, this result strongly suggests the reversibility of the RNAi effect. N.B: results from b) and d) come from the same flies, recorded at 2 and 5 days old respectively. e, f) Expressing the UAS-RNAi-HMGCR in the ca using the DI-11 line (DI-11/UAS-RNAi-HMGCR) also leads to the lack of the HMGCR product (f) and disrupts the sexual dimorphism compared to controls flies (e). g) The HMGCR is expressed both in the cardia and the ca (left panel) in control flies (2 days old UAS-RNAi-HMGCR/CS flies), whereas in 2 days old (DI-3/UAS-RNAi-HMGCR) flies, the HMGCR is detected only in the cardia (right panel). This last staining serves as a positive control, to demonstrate that the DI-3 drives the UAS-RNAi-HMGCR only in the ca. h) Expressing the UAS-RNAi-HMGCR in the ca under the control of DI-11 leads to a strong lethality when flies are reared at 24°C. Moreover, the only few females that survived are dwarf (left: dwarf female DI-11/UAS-RNAi-HMGCR, middle: female control UAS-RNAi-HMGCR/CS] and right: female CS). Scale bar = 25 µm. |
PMC1779623_pone-0000187-g005_8731.jpg | What object or scene is depicted here? | Directed expression of p[UAS-RNAi-HMGCR] specifically in the corpus allatum mimics the hmgcr mutation, by disrupting the sexual dimorphism.For all panels (a–g): immunostaining using a primary antibody raised against the human form of the HMGCR, revealed with a secondary antibody labeled with Cy3 (red). a–d) Targeting UAS-RNAi-HMGCR specifically in the ca yields to the lack of the HMGCR protein, and leads to the disruption of the sexual dimorphism. This effect is completely reversible. In 2 days old DI-3/UAS-RNAi-HMGCR flies (b), HMGCR protein is not detectable and the number of start/stop is identical between males and females compared to controls (a) (2 days old UAS-RNAi-HMGCR/CS flies). Interestingly, in 5 days old flies, both DI-3/UAS-RNAi-HMGCR (d) and UAS-RNAi-HMGCR/CS (c) the HMGCR is detectable and the start/stop number is sexually dimorphic. This is in agreement with the temporal expression pattern driven by the DI-3. Additionally, this result strongly suggests the reversibility of the RNAi effect. N.B: results from b) and d) come from the same flies, recorded at 2 and 5 days old respectively. e, f) Expressing the UAS-RNAi-HMGCR in the ca using the DI-11 line (DI-11/UAS-RNAi-HMGCR) also leads to the lack of the HMGCR product (f) and disrupts the sexual dimorphism compared to controls flies (e). g) The HMGCR is expressed both in the cardia and the ca (left panel) in control flies (2 days old UAS-RNAi-HMGCR/CS flies), whereas in 2 days old (DI-3/UAS-RNAi-HMGCR) flies, the HMGCR is detected only in the cardia (right panel). This last staining serves as a positive control, to demonstrate that the DI-3 drives the UAS-RNAi-HMGCR only in the ca. h) Expressing the UAS-RNAi-HMGCR in the ca under the control of DI-11 leads to a strong lethality when flies are reared at 24°C. Moreover, the only few females that survived are dwarf (left: dwarf female DI-11/UAS-RNAi-HMGCR, middle: female control UAS-RNAi-HMGCR/CS] and right: female CS). Scale bar = 25 µm. |
PMC1779623_pone-0000187-g005_8729.jpg | What is the core subject represented in this visual? | Directed expression of p[UAS-RNAi-HMGCR] specifically in the corpus allatum mimics the hmgcr mutation, by disrupting the sexual dimorphism.For all panels (a–g): immunostaining using a primary antibody raised against the human form of the HMGCR, revealed with a secondary antibody labeled with Cy3 (red). a–d) Targeting UAS-RNAi-HMGCR specifically in the ca yields to the lack of the HMGCR protein, and leads to the disruption of the sexual dimorphism. This effect is completely reversible. In 2 days old DI-3/UAS-RNAi-HMGCR flies (b), HMGCR protein is not detectable and the number of start/stop is identical between males and females compared to controls (a) (2 days old UAS-RNAi-HMGCR/CS flies). Interestingly, in 5 days old flies, both DI-3/UAS-RNAi-HMGCR (d) and UAS-RNAi-HMGCR/CS (c) the HMGCR is detectable and the start/stop number is sexually dimorphic. This is in agreement with the temporal expression pattern driven by the DI-3. Additionally, this result strongly suggests the reversibility of the RNAi effect. N.B: results from b) and d) come from the same flies, recorded at 2 and 5 days old respectively. e, f) Expressing the UAS-RNAi-HMGCR in the ca using the DI-11 line (DI-11/UAS-RNAi-HMGCR) also leads to the lack of the HMGCR product (f) and disrupts the sexual dimorphism compared to controls flies (e). g) The HMGCR is expressed both in the cardia and the ca (left panel) in control flies (2 days old UAS-RNAi-HMGCR/CS flies), whereas in 2 days old (DI-3/UAS-RNAi-HMGCR) flies, the HMGCR is detected only in the cardia (right panel). This last staining serves as a positive control, to demonstrate that the DI-3 drives the UAS-RNAi-HMGCR only in the ca. h) Expressing the UAS-RNAi-HMGCR in the ca under the control of DI-11 leads to a strong lethality when flies are reared at 24°C. Moreover, the only few females that survived are dwarf (left: dwarf female DI-11/UAS-RNAi-HMGCR, middle: female control UAS-RNAi-HMGCR/CS] and right: female CS). Scale bar = 25 µm. |
PMC1779623_pone-0000187-g006_8725.jpg | What is the focal point of this photograph? | Directed expression of p[UAS-RNAi-InR] specifically in the corpus allatum, mimics the hmgcr mutation.For all panels (a–c): immunostaining using a primary antibody raised against the human form of the InR, revealed by a secondary antibody labeled with FITC (green). a–c) Targeting the UAS-RNAi-InR specifically in the ca blocks the InR expression and disrupts the sexual dimorphism. This effect is completely reversible. In 2 days old DI-3/UAS-RNAi-InR flies (b), InR is not detectable and the number of start/stop is identical between males and females compared to controls flies (a) (2 days old UAS-RNAi-InR/CS). Interestingly, in 5 days old DI-3/UAS-RNAi-InR flies (c) the InR is detectable and the start/stop number is sexually dimorphic. This result corroborates the temporal expression pattern driven by the DI-3. Additionally, this strongly suggests, like for the RNAi-HMGCR, the reversibility of the RNAi-InR effect. N.B.: again here, results from b) and d) come from the same flies, recorded at 2 and 5 days old, respectively. d) Expressing the UAS-RNAi-InR in the ca under the control of DI-11 leads to a strong lethality when flies are reared at 24°C and 19°C. However, at 19°C, few females survive, but they are dwarf (left: dwarf female DI-11/UAS-RNAi-InR, right: female control (UAS-RNAi-InR/CS). Scale bar = 25 µm. |
PMC1779623_pone-0000187-g006_8726.jpg | What is shown in this image? | Directed expression of p[UAS-RNAi-InR] specifically in the corpus allatum, mimics the hmgcr mutation.For all panels (a–c): immunostaining using a primary antibody raised against the human form of the InR, revealed by a secondary antibody labeled with FITC (green). a–c) Targeting the UAS-RNAi-InR specifically in the ca blocks the InR expression and disrupts the sexual dimorphism. This effect is completely reversible. In 2 days old DI-3/UAS-RNAi-InR flies (b), InR is not detectable and the number of start/stop is identical between males and females compared to controls flies (a) (2 days old UAS-RNAi-InR/CS). Interestingly, in 5 days old DI-3/UAS-RNAi-InR flies (c) the InR is detectable and the start/stop number is sexually dimorphic. This result corroborates the temporal expression pattern driven by the DI-3. Additionally, this strongly suggests, like for the RNAi-HMGCR, the reversibility of the RNAi-InR effect. N.B.: again here, results from b) and d) come from the same flies, recorded at 2 and 5 days old, respectively. d) Expressing the UAS-RNAi-InR in the ca under the control of DI-11 leads to a strong lethality when flies are reared at 24°C and 19°C. However, at 19°C, few females survive, but they are dwarf (left: dwarf female DI-11/UAS-RNAi-InR, right: female control (UAS-RNAi-InR/CS). Scale bar = 25 µm. |
PMC1779780_F1_8743.jpg | What can you see in this picture? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
PMC1779780_F1_8742.jpg | What is the principal component of this image? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
PMC1779780_F1_8741.jpg | What is the central feature of this picture? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
PMC1779780_F1_8746.jpg | What is the core subject represented in this visual? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
PMC1779780_F1_8740.jpg | What does this image primarily show? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
PMC1779780_F1_8739.jpg | What's the most prominent thing you notice in this picture? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
PMC1779780_F1_8747.jpg | Can you identify the primary element in this image? | Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm. |
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