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PMC1793994_F8_9289.jpg | What's the most prominent thing you notice in this picture? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1793994_F8_9283.jpg | What can you see in this picture? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1793994_F8_9287.jpg | What is shown in this image? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1793994_F8_9285.jpg | Can you identify the primary element in this image? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1793994_F8_9290.jpg | What key item or scene is captured in this photo? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1793994_F8_9284.jpg | What object or scene is depicted here? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1793994_F8_9293.jpg | What is the focal point of this photograph? | Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens. |
PMC1794134_pone-0000229-g003_9296.jpg | What does this image primarily show? | Electron micrographs of transverse ultra-thin sections of aortas from wild-type mice housed in a standard cage (panel A) and an enriched cage (panel B). |
PMC1794165_pone-0000221-g001_9297.jpg | What's the most prominent thing you notice in this picture? | Incubation of rabbit retina.Photograph (A) and schematic diagram (B) of the hybrid interphase/perfusion chamber. Deep dishes are used with custom made “stands” of 1 cm height to support the tissue culture insert with the flat-mounted retina. The retina is in contact with the Ames' medium over the filter on the photoreceptor side; the ganglion cell side faces the atmosphere. (C) Low power micrograph of adult rabbit retina transfected with an expression plasmid for EGFP after 4 days in culture. Scale bar, 100 µm. (D) ON/OFF Directionally selective ganglion cell (Type G7, bistratified). All panels show maximum intensity projections of ∼20 individual image planes of a through-focus series taken at 1 µm steps on a confocal microscope. (E) ON directionally selective ganglion cell (G10). (F) Alpha cell. (G) Brisk sustained cell (G4). All scale bars, 100 µm. |
PMC1794165_pone-0000221-g001_9301.jpg | What is the principal component of this image? | Incubation of rabbit retina.Photograph (A) and schematic diagram (B) of the hybrid interphase/perfusion chamber. Deep dishes are used with custom made “stands” of 1 cm height to support the tissue culture insert with the flat-mounted retina. The retina is in contact with the Ames' medium over the filter on the photoreceptor side; the ganglion cell side faces the atmosphere. (C) Low power micrograph of adult rabbit retina transfected with an expression plasmid for EGFP after 4 days in culture. Scale bar, 100 µm. (D) ON/OFF Directionally selective ganglion cell (Type G7, bistratified). All panels show maximum intensity projections of ∼20 individual image planes of a through-focus series taken at 1 µm steps on a confocal microscope. (E) ON directionally selective ganglion cell (G10). (F) Alpha cell. (G) Brisk sustained cell (G4). All scale bars, 100 µm. |
PMC1794165_pone-0000221-g001_9303.jpg | What's the most prominent thing you notice in this picture? | Incubation of rabbit retina.Photograph (A) and schematic diagram (B) of the hybrid interphase/perfusion chamber. Deep dishes are used with custom made “stands” of 1 cm height to support the tissue culture insert with the flat-mounted retina. The retina is in contact with the Ames' medium over the filter on the photoreceptor side; the ganglion cell side faces the atmosphere. (C) Low power micrograph of adult rabbit retina transfected with an expression plasmid for EGFP after 4 days in culture. Scale bar, 100 µm. (D) ON/OFF Directionally selective ganglion cell (Type G7, bistratified). All panels show maximum intensity projections of ∼20 individual image planes of a through-focus series taken at 1 µm steps on a confocal microscope. (E) ON directionally selective ganglion cell (G10). (F) Alpha cell. (G) Brisk sustained cell (G4). All scale bars, 100 µm. |
PMC1794165_pone-0000221-g001_9302.jpg | What is the core subject represented in this visual? | Incubation of rabbit retina.Photograph (A) and schematic diagram (B) of the hybrid interphase/perfusion chamber. Deep dishes are used with custom made “stands” of 1 cm height to support the tissue culture insert with the flat-mounted retina. The retina is in contact with the Ames' medium over the filter on the photoreceptor side; the ganglion cell side faces the atmosphere. (C) Low power micrograph of adult rabbit retina transfected with an expression plasmid for EGFP after 4 days in culture. Scale bar, 100 µm. (D) ON/OFF Directionally selective ganglion cell (Type G7, bistratified). All panels show maximum intensity projections of ∼20 individual image planes of a through-focus series taken at 1 µm steps on a confocal microscope. (E) ON directionally selective ganglion cell (G10). (F) Alpha cell. (G) Brisk sustained cell (G4). All scale bars, 100 µm. |
PMC1794165_pone-0000221-g001_9299.jpg | Describe the main subject of this image. | Incubation of rabbit retina.Photograph (A) and schematic diagram (B) of the hybrid interphase/perfusion chamber. Deep dishes are used with custom made “stands” of 1 cm height to support the tissue culture insert with the flat-mounted retina. The retina is in contact with the Ames' medium over the filter on the photoreceptor side; the ganglion cell side faces the atmosphere. (C) Low power micrograph of adult rabbit retina transfected with an expression plasmid for EGFP after 4 days in culture. Scale bar, 100 µm. (D) ON/OFF Directionally selective ganglion cell (Type G7, bistratified). All panels show maximum intensity projections of ∼20 individual image planes of a through-focus series taken at 1 µm steps on a confocal microscope. (E) ON directionally selective ganglion cell (G10). (F) Alpha cell. (G) Brisk sustained cell (G4). All scale bars, 100 µm. |
PMC1794227_F2_9307.jpg | What object or scene is depicted here? | Immunohistochemistry of lungs on day 7 after infection with HMPV or RSV. (A) Normal lung tissue from an uninfected animal. (B) Pulmonary section from an RSV-infected mouse showing mild bronchopneumonia with scattered macrophages and neutrophils in alveolar spaces. (C) Severe bronchopneumonia in a mouse inoculated with HMPV. Bronchioli and adjacent alveoli are densely infiltrated by macrophages and neutrophils admixed with fibrin. (D) Immunohistochemical staining for HMPV. Groups of intraalveolar macrophages and pneumocytes expressing HMPV antigens. (A to D): hematoxylin-eosin staining, original magnification × 20; D: immunostaining with anti-HMPV serum, (× 63). Representative sections from groups of 4 mice are shown. |
PMC1794227_F2_9306.jpg | Describe the main subject of this image. | Immunohistochemistry of lungs on day 7 after infection with HMPV or RSV. (A) Normal lung tissue from an uninfected animal. (B) Pulmonary section from an RSV-infected mouse showing mild bronchopneumonia with scattered macrophages and neutrophils in alveolar spaces. (C) Severe bronchopneumonia in a mouse inoculated with HMPV. Bronchioli and adjacent alveoli are densely infiltrated by macrophages and neutrophils admixed with fibrin. (D) Immunohistochemical staining for HMPV. Groups of intraalveolar macrophages and pneumocytes expressing HMPV antigens. (A to D): hematoxylin-eosin staining, original magnification × 20; D: immunostaining with anti-HMPV serum, (× 63). Representative sections from groups of 4 mice are shown. |
PMC1794227_F2_9304.jpg | What is being portrayed in this visual content? | Immunohistochemistry of lungs on day 7 after infection with HMPV or RSV. (A) Normal lung tissue from an uninfected animal. (B) Pulmonary section from an RSV-infected mouse showing mild bronchopneumonia with scattered macrophages and neutrophils in alveolar spaces. (C) Severe bronchopneumonia in a mouse inoculated with HMPV. Bronchioli and adjacent alveoli are densely infiltrated by macrophages and neutrophils admixed with fibrin. (D) Immunohistochemical staining for HMPV. Groups of intraalveolar macrophages and pneumocytes expressing HMPV antigens. (A to D): hematoxylin-eosin staining, original magnification × 20; D: immunostaining with anti-HMPV serum, (× 63). Representative sections from groups of 4 mice are shown. |
PMC1794227_F2_9305.jpg | What's the most prominent thing you notice in this picture? | Immunohistochemistry of lungs on day 7 after infection with HMPV or RSV. (A) Normal lung tissue from an uninfected animal. (B) Pulmonary section from an RSV-infected mouse showing mild bronchopneumonia with scattered macrophages and neutrophils in alveolar spaces. (C) Severe bronchopneumonia in a mouse inoculated with HMPV. Bronchioli and adjacent alveoli are densely infiltrated by macrophages and neutrophils admixed with fibrin. (D) Immunohistochemical staining for HMPV. Groups of intraalveolar macrophages and pneumocytes expressing HMPV antigens. (A to D): hematoxylin-eosin staining, original magnification × 20; D: immunostaining with anti-HMPV serum, (× 63). Representative sections from groups of 4 mice are shown. |
PMC1794233_F2_9309.jpg | What is being portrayed in this visual content? | Acute LV systolic dysfunction in a 50-year old female patient affected by granulocytic sarcoma during CT by daunorubicine (50 mg/m2), cytarabine (30 mg/m2), etoposide 50 mg/m2. LV end-diastolic diameter (left panel) is 48.9 mm and LV end-systolic diameter (right panel) is 41.7 mm. Thus, endocardial fractional shortening is 14.7 % and EF = 37.9 %. EF = Ejection fraction, LV = Left ventricular. |
PMC1794233_F2_9308.jpg | What is the core subject represented in this visual? | Acute LV systolic dysfunction in a 50-year old female patient affected by granulocytic sarcoma during CT by daunorubicine (50 mg/m2), cytarabine (30 mg/m2), etoposide 50 mg/m2. LV end-diastolic diameter (left panel) is 48.9 mm and LV end-systolic diameter (right panel) is 41.7 mm. Thus, endocardial fractional shortening is 14.7 % and EF = 37.9 %. EF = Ejection fraction, LV = Left ventricular. |
PMC1794233_F4_9313.jpg | What does this image primarily show? | Detection of posterior pericardial effusion in a 28-year old female patient affected by non Hodgkin's lymphoma and treated by CHOP (Cyclophosphamide 750 mg/m 2 + Adriamycin 50 mg/m 2 + Vincristine 2 mg + Prednisone 100 mg) and previous mediastinal radiation therapy. The usual grading of pericardial effusion takes into account the diastolic separation between epicardium and pericardium: 1. small = < 10 mm corresponding to 300 ml, 2. moderate = 10–20 mm corresponding to 500 ml, 3. severe = > 20 mm corresponding to > 700 ml). In this patient the diastolic separation is < 10 mm (both in long and in short-axis vies), indicating mild pericardial effusion. Upper panel; visualization of diastolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). Lower panel: visualization of systolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). |
PMC1794233_F4_9312.jpg | Describe the main subject of this image. | Detection of posterior pericardial effusion in a 28-year old female patient affected by non Hodgkin's lymphoma and treated by CHOP (Cyclophosphamide 750 mg/m 2 + Adriamycin 50 mg/m 2 + Vincristine 2 mg + Prednisone 100 mg) and previous mediastinal radiation therapy. The usual grading of pericardial effusion takes into account the diastolic separation between epicardium and pericardium: 1. small = < 10 mm corresponding to 300 ml, 2. moderate = 10–20 mm corresponding to 500 ml, 3. severe = > 20 mm corresponding to > 700 ml). In this patient the diastolic separation is < 10 mm (both in long and in short-axis vies), indicating mild pericardial effusion. Upper panel; visualization of diastolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). Lower panel: visualization of systolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). |
PMC1794233_F4_9311.jpg | What is the dominant medical problem in this image? | Detection of posterior pericardial effusion in a 28-year old female patient affected by non Hodgkin's lymphoma and treated by CHOP (Cyclophosphamide 750 mg/m 2 + Adriamycin 50 mg/m 2 + Vincristine 2 mg + Prednisone 100 mg) and previous mediastinal radiation therapy. The usual grading of pericardial effusion takes into account the diastolic separation between epicardium and pericardium: 1. small = < 10 mm corresponding to 300 ml, 2. moderate = 10–20 mm corresponding to 500 ml, 3. severe = > 20 mm corresponding to > 700 ml). In this patient the diastolic separation is < 10 mm (both in long and in short-axis vies), indicating mild pericardial effusion. Upper panel; visualization of diastolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). Lower panel: visualization of systolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). |
PMC1794233_F4_9310.jpg | What is the main focus of this visual representation? | Detection of posterior pericardial effusion in a 28-year old female patient affected by non Hodgkin's lymphoma and treated by CHOP (Cyclophosphamide 750 mg/m 2 + Adriamycin 50 mg/m 2 + Vincristine 2 mg + Prednisone 100 mg) and previous mediastinal radiation therapy. The usual grading of pericardial effusion takes into account the diastolic separation between epicardium and pericardium: 1. small = < 10 mm corresponding to 300 ml, 2. moderate = 10–20 mm corresponding to 500 ml, 3. severe = > 20 mm corresponding to > 700 ml). In this patient the diastolic separation is < 10 mm (both in long and in short-axis vies), indicating mild pericardial effusion. Upper panel; visualization of diastolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). Lower panel: visualization of systolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). |
PMC1794249_F1_9317.jpg | What stands out most in this visual? | Identification of cultured adult rat RGCs. Cultured cells were co-labeled with anti-Thy-1 antibody (A), anti-NF-L antibody (B), and DAPI (C). (D) A digitally merged image simultaneously represents all three fluorescent labels. (E) The corresponding phase-contrast image. The cells were cultured for 3 days under control conditions. Scale bar = 50 μm. |
PMC1794249_F1_9318.jpg | What is the focal point of this photograph? | Identification of cultured adult rat RGCs. Cultured cells were co-labeled with anti-Thy-1 antibody (A), anti-NF-L antibody (B), and DAPI (C). (D) A digitally merged image simultaneously represents all three fluorescent labels. (E) The corresponding phase-contrast image. The cells were cultured for 3 days under control conditions. Scale bar = 50 μm. |
PMC1794249_F1_9314.jpg | What is shown in this image? | Identification of cultured adult rat RGCs. Cultured cells were co-labeled with anti-Thy-1 antibody (A), anti-NF-L antibody (B), and DAPI (C). (D) A digitally merged image simultaneously represents all three fluorescent labels. (E) The corresponding phase-contrast image. The cells were cultured for 3 days under control conditions. Scale bar = 50 μm. |
PMC1794249_F2_9323.jpg | What is the main focus of this visual representation? | Morphology of cultured adult rat RGCs. The cells, cultured for 3 (A & B), 7 (C & D), and 11 (E & F) days, were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B), (D), are (F) are the corresponding phase-contrast images. Scale bar = 50 μm. |
PMC1794249_F2_9322.jpg | What can you see in this picture? | Morphology of cultured adult rat RGCs. The cells, cultured for 3 (A & B), 7 (C & D), and 11 (E & F) days, were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B), (D), are (F) are the corresponding phase-contrast images. Scale bar = 50 μm. |
PMC1794249_F2_9319.jpg | What can you see in this picture? | Morphology of cultured adult rat RGCs. The cells, cultured for 3 (A & B), 7 (C & D), and 11 (E & F) days, were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B), (D), are (F) are the corresponding phase-contrast images. Scale bar = 50 μm. |
PMC1794249_F2_9321.jpg | What is shown in this image? | Morphology of cultured adult rat RGCs. The cells, cultured for 3 (A & B), 7 (C & D), and 11 (E & F) days, were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B), (D), are (F) are the corresponding phase-contrast images. Scale bar = 50 μm. |
PMC1794249_F2_9320.jpg | What is the dominant medical problem in this image? | Morphology of cultured adult rat RGCs. The cells, cultured for 3 (A & B), 7 (C & D), and 11 (E & F) days, were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B), (D), are (F) are the corresponding phase-contrast images. Scale bar = 50 μm. |
PMC1794250_F2_9327.jpg | What does this image primarily show? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9333.jpg | What can you see in this picture? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9325.jpg | Can you identify the primary element in this image? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9330.jpg | What object or scene is depicted here? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9332.jpg | What is being portrayed in this visual content? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9328.jpg | What stands out most in this visual? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9326.jpg | What can you see in this picture? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9329.jpg | Describe the main subject of this image. | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9331.jpg | What is the principal component of this image? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794250_F2_9334.jpg | What does this image primarily show? | Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J). |
PMC1794257_F6_9337.jpg | What key item or scene is captured in this photo? | (A): HSIL (Pap Test) (100×); (B): Cervical intraepithelial neoplasia 2 (CIN2) on biopsy (40×). |
PMC1794257_F6_9335.jpg | Can you identify the primary element in this image? | (A): HSIL (Pap Test) (100×); (B): Cervical intraepithelial neoplasia 2 (CIN2) on biopsy (40×). |
PMC1794319_pone-0000234-g001_9340.jpg | What is the core subject represented in this visual? | External morphology of the honey bee antenna. The honey bee antenna was examined by scanning electron microscopy with different magnifications (A; x40, B; x150, C; x 300). Two proximal antennal segments (scape and pedicel) and the ten segments of the flagellum are indicated by arrows in A. Arrowhead in C indicates the position of electrode insertion for SEP recordings. The scale of each panel is shown by a white bar. |
PMC1794319_pone-0000234-g001_9339.jpg | What object or scene is depicted here? | External morphology of the honey bee antenna. The honey bee antenna was examined by scanning electron microscopy with different magnifications (A; x40, B; x150, C; x 300). Two proximal antennal segments (scape and pedicel) and the ten segments of the flagellum are indicated by arrows in A. Arrowhead in C indicates the position of electrode insertion for SEP recordings. The scale of each panel is shown by a white bar. |
PMC1794407_F6_9346.jpg | What does this image primarily show? | Localization and kinetics of dephosphorylation of Borealin in cells that fail to divide. HEK293 cells stably expressing Borealin-GFP were exposed to blebbistatin (41 μM). The localization of Borealin and α-tubulin were analyzed by confocal microscopy. (A) Untreated, (B and C) Blebbistatin for 3.8 hrs (D and E) Blebbistatin for 45 min. (F) Borealin dephosphorylation and scheme of the experiment. WT-8 cells were synchronized in mitosis with nocodazole and then released from the block in the presence or absence of blebbistatin. Lysates were collected at the times indicated and analyzed by Western blotting. Western transfers were stripped and reprobed for β-actin as a loading control. |
PMC1794407_F6_9342.jpg | What is the main focus of this visual representation? | Localization and kinetics of dephosphorylation of Borealin in cells that fail to divide. HEK293 cells stably expressing Borealin-GFP were exposed to blebbistatin (41 μM). The localization of Borealin and α-tubulin were analyzed by confocal microscopy. (A) Untreated, (B and C) Blebbistatin for 3.8 hrs (D and E) Blebbistatin for 45 min. (F) Borealin dephosphorylation and scheme of the experiment. WT-8 cells were synchronized in mitosis with nocodazole and then released from the block in the presence or absence of blebbistatin. Lysates were collected at the times indicated and analyzed by Western blotting. Western transfers were stripped and reprobed for β-actin as a loading control. |
PMC1794407_F6_9343.jpg | Can you identify the primary element in this image? | Localization and kinetics of dephosphorylation of Borealin in cells that fail to divide. HEK293 cells stably expressing Borealin-GFP were exposed to blebbistatin (41 μM). The localization of Borealin and α-tubulin were analyzed by confocal microscopy. (A) Untreated, (B and C) Blebbistatin for 3.8 hrs (D and E) Blebbistatin for 45 min. (F) Borealin dephosphorylation and scheme of the experiment. WT-8 cells were synchronized in mitosis with nocodazole and then released from the block in the presence or absence of blebbistatin. Lysates were collected at the times indicated and analyzed by Western blotting. Western transfers were stripped and reprobed for β-actin as a loading control. |
PMC1794407_F6_9344.jpg | What is shown in this image? | Localization and kinetics of dephosphorylation of Borealin in cells that fail to divide. HEK293 cells stably expressing Borealin-GFP were exposed to blebbistatin (41 μM). The localization of Borealin and α-tubulin were analyzed by confocal microscopy. (A) Untreated, (B and C) Blebbistatin for 3.8 hrs (D and E) Blebbistatin for 45 min. (F) Borealin dephosphorylation and scheme of the experiment. WT-8 cells were synchronized in mitosis with nocodazole and then released from the block in the presence or absence of blebbistatin. Lysates were collected at the times indicated and analyzed by Western blotting. Western transfers were stripped and reprobed for β-actin as a loading control. |
PMC1794409_F2_9347.jpg | What key item or scene is captured in this photo? | Measurements of intervertebral displacement resulting from the posterior to anterior (PA) mobilization and the prone press-up (PU) maneuver. The intervertebral (segmental) angle was measured as the angle formed by lines defining the endplates of adjacent vertebrae. Segmental lumbar displacement was defined as the difference in the intervertebral angle between the resting position (left) and intervertebral angle from the end range image (right). The arrow in Figure 2a identifies the hand of the examiner performing the PA mobilization. |
PMC1794409_F2_9350.jpg | What is the focal point of this photograph? | Measurements of intervertebral displacement resulting from the posterior to anterior (PA) mobilization and the prone press-up (PU) maneuver. The intervertebral (segmental) angle was measured as the angle formed by lines defining the endplates of adjacent vertebrae. Segmental lumbar displacement was defined as the difference in the intervertebral angle between the resting position (left) and intervertebral angle from the end range image (right). The arrow in Figure 2a identifies the hand of the examiner performing the PA mobilization. |
PMC1794409_F2_9348.jpg | What is the main focus of this visual representation? | Measurements of intervertebral displacement resulting from the posterior to anterior (PA) mobilization and the prone press-up (PU) maneuver. The intervertebral (segmental) angle was measured as the angle formed by lines defining the endplates of adjacent vertebrae. Segmental lumbar displacement was defined as the difference in the intervertebral angle between the resting position (left) and intervertebral angle from the end range image (right). The arrow in Figure 2a identifies the hand of the examiner performing the PA mobilization. |
PMC1794409_F2_9349.jpg | What is the main focus of this visual representation? | Measurements of intervertebral displacement resulting from the posterior to anterior (PA) mobilization and the prone press-up (PU) maneuver. The intervertebral (segmental) angle was measured as the angle formed by lines defining the endplates of adjacent vertebrae. Segmental lumbar displacement was defined as the difference in the intervertebral angle between the resting position (left) and intervertebral angle from the end range image (right). The arrow in Figure 2a identifies the hand of the examiner performing the PA mobilization. |
PMC1794416_F3_9355.jpg | What does this image primarily show? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9371.jpg | What is shown in this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9364.jpg | What can you see in this picture? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9372.jpg | What key item or scene is captured in this photo? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9354.jpg | What is shown in this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9359.jpg | What is the main focus of this visual representation? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9370.jpg | What key item or scene is captured in this photo? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9351.jpg | What is the main focus of this visual representation? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9368.jpg | What is the dominant medical problem in this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9366.jpg | What is the dominant medical problem in this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9363.jpg | What object or scene is depicted here? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9362.jpg | What is the central feature of this picture? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9367.jpg | What's the most prominent thing you notice in this picture? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9352.jpg | What is the dominant medical problem in this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9356.jpg | What is the principal component of this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9365.jpg | What can you see in this picture? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794416_F3_9358.jpg | Can you identify the primary element in this image? | Immunohistochemical analysis of NIS expression in human gastrointestinal tract. a: normal esophageal squamous epithelium (haematoxylin-eosin/HE/staining, original magnification 100×) b: normal esophageal squamous epithelium: negative for NIS expression (original magnification 100×) c: Barrett mucosa (HE staining, original magnification 100×) d: Barrett mucosa, junctional and fundus-type columnar metaplasia: NIS positive staining (original magnification 100×) e: Barrett mucosa with intestinal metaplasia (HE staining, original magnification 100×) f: Barrett mucosa with intestinal metaplasia: negative for NIS expression (original magnification 100×) g: squamous cell esophageal carcinoma (HE staining, original magnification 100×) h: squamous cell esophageal carcinoma: NIS negative staining (original magnification 100×) i: gastric carcinoma – signet ring cell – (HE staining, original magnification 200×) j: gastric carcinoma – signet ring cell – negative for NIS expression (original magnification 200×) k: on the border of the gastric adenocarcinoma, adjacent "normal" extratumoral mucosa: faint, focal NIS expression (original magnification 100×) I: at 1 cm from gastric adenocarcinoma: definite focal NIS staining (original magnification 400×) m: far from the gastric adenocarcinoma: strong, linear NIS expression (original magnification 400×) n: gastric polyp (HE staining, original magnification 100×) o: gastric polyp: NIS positive staining (original magnification 100×) p: gastric polyp (HE staining, original magnification 200×) q: gastric polyp with colon-type metaplasia: NIS negative staining (original magnification 200×) r: small bowel adenocarcinoma (HE staining, original magnification 100×) s: small bowel adenocarcinoma: NIS negative staining (original magnification 100×) t: normal large bowel mucosa: NIS negative staining (original magnification 100×) v: colon polyp (HE staining, original magnification 100×) w: colon polyp: NIS negative expression (original magnification 100×) x: adenocarcinoma of the colon (HE staining, original magnification 100×) y: adenocarcinoma of the colon: negative for NIS expression (original magnification 100×). |
PMC1794429_F5_9376.jpg | What is the central feature of this picture? | Typical kidney tumor as found in p53E241X/E241X homozygous fish. (a) A stereoscopic view of the kidney tumor identified in a 2.5 month old homozygous p53E241X/E241X fish. (b-d) Hematoxylin-eosin staining of normal (b) and neoplastic (c) kidney of medaka. Note that the interstitial tissue is infiltrated with numerous hematopoietic cells destroying the normal architecture of renal tubules. The higher magnification shows the mixture of small lymphocytes with little cytoplasm and the plasmacyte-like cells with large basophilic cytoplasm (d). |
PMC1794429_F5_9374.jpg | What stands out most in this visual? | Typical kidney tumor as found in p53E241X/E241X homozygous fish. (a) A stereoscopic view of the kidney tumor identified in a 2.5 month old homozygous p53E241X/E241X fish. (b-d) Hematoxylin-eosin staining of normal (b) and neoplastic (c) kidney of medaka. Note that the interstitial tissue is infiltrated with numerous hematopoietic cells destroying the normal architecture of renal tubules. The higher magnification shows the mixture of small lymphocytes with little cytoplasm and the plasmacyte-like cells with large basophilic cytoplasm (d). |
PMC1794429_F5_9373.jpg | What stands out most in this visual? | Typical kidney tumor as found in p53E241X/E241X homozygous fish. (a) A stereoscopic view of the kidney tumor identified in a 2.5 month old homozygous p53E241X/E241X fish. (b-d) Hematoxylin-eosin staining of normal (b) and neoplastic (c) kidney of medaka. Note that the interstitial tissue is infiltrated with numerous hematopoietic cells destroying the normal architecture of renal tubules. The higher magnification shows the mixture of small lymphocytes with little cytoplasm and the plasmacyte-like cells with large basophilic cytoplasm (d). |
PMC1794436_F2_9388.jpg | What is the dominant medical problem in this image? | Comparing three-dimensional raw images to PointCloud representations. (a-c) Maximum projections of the three channels of a three-dimensional embryo image; (a) the nuclear stain (white); (b) a snail mRNA stain (red); and (c) an eve mRNA stain (green). Note the small bright speckles visible in all three channels at the same locations. These are outside the cytoplasm and are detected and removed by our image analysis algorithms. The small white rectangles show a region of interest that is displayed in (d-g). (d,e) The raw image of the nuclear stain (d) and the mRNA stains for eve and sna (e). (f,g) Two different renderings of the PointCloud derived from this image made using our visualization tool PointCloudXplore: (f) uses small spheres whose volumes are proportional to the measured volumes of the corresponding nuclei; (g) uses a Voronoi tessellation of the coordinates in the PointCloud. The arrows indicate the locations of the same three cells in each of the panels (d-g). |
PMC1794436_F2_9386.jpg | What key item or scene is captured in this photo? | Comparing three-dimensional raw images to PointCloud representations. (a-c) Maximum projections of the three channels of a three-dimensional embryo image; (a) the nuclear stain (white); (b) a snail mRNA stain (red); and (c) an eve mRNA stain (green). Note the small bright speckles visible in all three channels at the same locations. These are outside the cytoplasm and are detected and removed by our image analysis algorithms. The small white rectangles show a region of interest that is displayed in (d-g). (d,e) The raw image of the nuclear stain (d) and the mRNA stains for eve and sna (e). (f,g) Two different renderings of the PointCloud derived from this image made using our visualization tool PointCloudXplore: (f) uses small spheres whose volumes are proportional to the measured volumes of the corresponding nuclei; (g) uses a Voronoi tessellation of the coordinates in the PointCloud. The arrows indicate the locations of the same three cells in each of the panels (d-g). |
PMC1794436_F2_9383.jpg | Can you identify the primary element in this image? | Comparing three-dimensional raw images to PointCloud representations. (a-c) Maximum projections of the three channels of a three-dimensional embryo image; (a) the nuclear stain (white); (b) a snail mRNA stain (red); and (c) an eve mRNA stain (green). Note the small bright speckles visible in all three channels at the same locations. These are outside the cytoplasm and are detected and removed by our image analysis algorithms. The small white rectangles show a region of interest that is displayed in (d-g). (d,e) The raw image of the nuclear stain (d) and the mRNA stains for eve and sna (e). (f,g) Two different renderings of the PointCloud derived from this image made using our visualization tool PointCloudXplore: (f) uses small spheres whose volumes are proportional to the measured volumes of the corresponding nuclei; (g) uses a Voronoi tessellation of the coordinates in the PointCloud. The arrows indicate the locations of the same three cells in each of the panels (d-g). |
PMC1794436_F2_9384.jpg | What is the main focus of this visual representation? | Comparing three-dimensional raw images to PointCloud representations. (a-c) Maximum projections of the three channels of a three-dimensional embryo image; (a) the nuclear stain (white); (b) a snail mRNA stain (red); and (c) an eve mRNA stain (green). Note the small bright speckles visible in all three channels at the same locations. These are outside the cytoplasm and are detected and removed by our image analysis algorithms. The small white rectangles show a region of interest that is displayed in (d-g). (d,e) The raw image of the nuclear stain (d) and the mRNA stains for eve and sna (e). (f,g) Two different renderings of the PointCloud derived from this image made using our visualization tool PointCloudXplore: (f) uses small spheres whose volumes are proportional to the measured volumes of the corresponding nuclei; (g) uses a Voronoi tessellation of the coordinates in the PointCloud. The arrows indicate the locations of the same three cells in each of the panels (d-g). |
PMC1794436_F2_9382.jpg | What is the principal component of this image? | Comparing three-dimensional raw images to PointCloud representations. (a-c) Maximum projections of the three channels of a three-dimensional embryo image; (a) the nuclear stain (white); (b) a snail mRNA stain (red); and (c) an eve mRNA stain (green). Note the small bright speckles visible in all three channels at the same locations. These are outside the cytoplasm and are detected and removed by our image analysis algorithms. The small white rectangles show a region of interest that is displayed in (d-g). (d,e) The raw image of the nuclear stain (d) and the mRNA stains for eve and sna (e). (f,g) Two different renderings of the PointCloud derived from this image made using our visualization tool PointCloudXplore: (f) uses small spheres whose volumes are proportional to the measured volumes of the corresponding nuclei; (g) uses a Voronoi tessellation of the coordinates in the PointCloud. The arrows indicate the locations of the same three cells in each of the panels (d-g). |
PMC1794436_F2_9387.jpg | Describe the main subject of this image. | Comparing three-dimensional raw images to PointCloud representations. (a-c) Maximum projections of the three channels of a three-dimensional embryo image; (a) the nuclear stain (white); (b) a snail mRNA stain (red); and (c) an eve mRNA stain (green). Note the small bright speckles visible in all three channels at the same locations. These are outside the cytoplasm and are detected and removed by our image analysis algorithms. The small white rectangles show a region of interest that is displayed in (d-g). (d,e) The raw image of the nuclear stain (d) and the mRNA stains for eve and sna (e). (f,g) Two different renderings of the PointCloud derived from this image made using our visualization tool PointCloudXplore: (f) uses small spheres whose volumes are proportional to the measured volumes of the corresponding nuclei; (g) uses a Voronoi tessellation of the coordinates in the PointCloud. The arrows indicate the locations of the same three cells in each of the panels (d-g). |
PMC1794436_F3_9380.jpg | What is being portrayed in this visual content? | Comparing segmentation results on the top and the side. Using a maximum projection, we show two portions of a three-dimensional image of an embryo fluorescently stained to label nuclei. (a) A projection along the optical axis, yielding a x-y image (the top of the embryo); (b) a projection perpendicular to that, yielding a x-z image (the side of the embryo). The nuclei on the top of the embryo appear well separated and distinct (a). Seen from the side, however, individual nuclei appear elongated along the z-axis due to limited axial resolution, which makes them more difficult to identify (b). The segmentation algorithm provided an accurate segmentation of nuclei (c) on the tops of embryo images, but (d) on the sides, a model was used to fine-tune the segmentation, resulting in a less accurate result. |
PMC1794436_F3_9381.jpg | What is shown in this image? | Comparing segmentation results on the top and the side. Using a maximum projection, we show two portions of a three-dimensional image of an embryo fluorescently stained to label nuclei. (a) A projection along the optical axis, yielding a x-y image (the top of the embryo); (b) a projection perpendicular to that, yielding a x-z image (the side of the embryo). The nuclei on the top of the embryo appear well separated and distinct (a). Seen from the side, however, individual nuclei appear elongated along the z-axis due to limited axial resolution, which makes them more difficult to identify (b). The segmentation algorithm provided an accurate segmentation of nuclei (c) on the tops of embryo images, but (d) on the sides, a model was used to fine-tune the segmentation, resulting in a less accurate result. |
PMC1794436_F3_9379.jpg | What key item or scene is captured in this photo? | Comparing segmentation results on the top and the side. Using a maximum projection, we show two portions of a three-dimensional image of an embryo fluorescently stained to label nuclei. (a) A projection along the optical axis, yielding a x-y image (the top of the embryo); (b) a projection perpendicular to that, yielding a x-z image (the side of the embryo). The nuclei on the top of the embryo appear well separated and distinct (a). Seen from the side, however, individual nuclei appear elongated along the z-axis due to limited axial resolution, which makes them more difficult to identify (b). The segmentation algorithm provided an accurate segmentation of nuclei (c) on the tops of embryo images, but (d) on the sides, a model was used to fine-tune the segmentation, resulting in a less accurate result. |
PMC1794436_F5_9377.jpg | What is shown in this image? | Patterns of nuclear displacement from the PointCloud surface. The location of each nucleus with respect to a smooth PointCloud surface was mapped and averaged over the same cohort of embryos used in Figure 3 and displayed as a cylindrical projection. The map shows that the average apical (positive) or basal (negative) shift of nuclei forms a pattern that appears to correlate with cell fate and the expression patterns of blastoderm transcriptional regulators. Egg length (EL). |
PMC1794488_F1_9390.jpg | What is shown in this image? | Transoesophageal echocardiographic transgastric, cross-sectional view of the left ventricle at midpapillary muscle level with automated border detection (ABD). Endocardial border of the left ventricle, including the papillary muscles, was circumscribed manually to define the region of interest (blue line). ABD quantifies the cardiac chamber areas instantaneously by detecting the blood-tissue interface (red line), which results in a continuous, beat-to-beat left ventricular area curve (green line). Left ventricular end-diastolic area (LVEDA) was defined as peak of the left ventricular area during diastole. Left ventricular end-systolic area (LVESA) was defined as minimum left ventricular area during systole. Stroke area (SA) was defined as LVEDA – LVESA over the same cardiac cycle. |
PMC1794488_F1_9389.jpg | What object or scene is depicted here? | Transoesophageal echocardiographic transgastric, cross-sectional view of the left ventricle at midpapillary muscle level with automated border detection (ABD). Endocardial border of the left ventricle, including the papillary muscles, was circumscribed manually to define the region of interest (blue line). ABD quantifies the cardiac chamber areas instantaneously by detecting the blood-tissue interface (red line), which results in a continuous, beat-to-beat left ventricular area curve (green line). Left ventricular end-diastolic area (LVEDA) was defined as peak of the left ventricular area during diastole. Left ventricular end-systolic area (LVESA) was defined as minimum left ventricular area during systole. Stroke area (SA) was defined as LVEDA – LVESA over the same cardiac cycle. |
PMC1794488_F2_9391.jpg | What is the principal component of this image? | Transoesophageal echocardiographic transgastric, cross-sectional views of the left ventricle at midpapillary muscle level with automated border detection at baseline (top panel) and after volume expansion induced by passive leg raising manoeuvre (bottom panel). Left ventricular area curve was displayed with electrocardiogram and respiratory curve. Stroke area (SA) was defined as the difference between the end-diastolic area (LVEDA) and the end-systolic area. Maximal (SAmax) and minimal (SAmin) values of pulse pressure were determined over the same respiratory cycle. Respiratory variations in left ventricular SA (ΔSA) were then calculated using the following formula: ΔSA = [(SAmax - SAmin)/([SAmax + SAmin]/2)] × 100%. Passive leg raising manoeuvre induced a decrease in ΔSA and an increase in LVEDA. Gain was held constant throughout the protocol. |
PMC1794488_F2_9392.jpg | What is the dominant medical problem in this image? | Transoesophageal echocardiographic transgastric, cross-sectional views of the left ventricle at midpapillary muscle level with automated border detection at baseline (top panel) and after volume expansion induced by passive leg raising manoeuvre (bottom panel). Left ventricular area curve was displayed with electrocardiogram and respiratory curve. Stroke area (SA) was defined as the difference between the end-diastolic area (LVEDA) and the end-systolic area. Maximal (SAmax) and minimal (SAmin) values of pulse pressure were determined over the same respiratory cycle. Respiratory variations in left ventricular SA (ΔSA) were then calculated using the following formula: ΔSA = [(SAmax - SAmin)/([SAmax + SAmin]/2)] × 100%. Passive leg raising manoeuvre induced a decrease in ΔSA and an increase in LVEDA. Gain was held constant throughout the protocol. |
PMC1794497_F2_9396.jpg | What is the central feature of this picture? | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794497_F2_9399.jpg | What is shown in this image? | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794497_F2_9400.jpg | Describe the main subject of this image. | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794497_F2_9397.jpg | What is the core subject represented in this visual? | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794497_F2_9393.jpg | What does this image primarily show? | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794497_F2_9394.jpg | What is the principal component of this image? | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794497_F2_9395.jpg | What object or scene is depicted here? | Development of infiltrates and inflammatory lesions in the vicinity of sunburn cells (SBCs) in patients with systemic lupus erythematosus (SLE). Haematoxylin eosin (H&E)-stained paraffin sections before and after irradiation with two minimal erythemal doses of ultraviolet B light (UVB). (a-c) Biopsies from a representative control, non-irradiated (a) and 1 (b) and 3 (c) days after irradiation. (d-f) Biopsies from a representative patient with increased influx of cells, non-irradiated (d) and 1 (e) and 3 (f) days after irradiation. Magnifications, ×100. (g) Graph showing semi-quantitative analysis of infiltrate in H&E sections before and up to 10 days after irradiation. Dotted lines indicate mean + two standard deviations of controls. ●, patients (P); ○, controls (C). No significant differences were present between patients and controls on any time point. (h) Inflammatory lesion in a patient with SLE in the vicinity of SBCs 3 days after irradiation. Inflammatory lesions were defined as the presence of category 5 (see Materials and methods) vessel(s) in the dermis, with inflammatory cell infiltration of the epidermal layer coinciding with marked local hydropic degeneration of the basal layer of the epidermis. Insert shows magnification of area with accumulating SBCs. Magnification, ×100. White arrowheads indicate SBCs, and black arrowheads indicate hydropic degeneration. |
PMC1794508_F1_9401.jpg | What stands out most in this visual? | Haematoxylin and eosin staining on healthy and arthritic synovial tissue. Haematoxylin and eosin staining was performed on synovial biopsies from (a) healthy subjects and (b) Rheumatoid arthritis patients. Arrows indicate synovial lining. |
PMC1794508_F3_9406.jpg | Describe the main subject of this image. | Collagen type IV staining in healthy and arthritic synovial tissue. Collagen type IV was found in the lining layer of synovial biopsies from (a) healthy human subjects and (c) rheumatoid arthritis (RA) patients. Relative isotype controls are shown in (b,d). Double staining was performed to reveal the cellular origin of the type IV collagen. (e) Double staining was found in the lining layer. For a clearer picture, succeeding slices were stained with (f) CD55 or (g) collagen type IV. (h) Isotype control. Arrows indicate collagen IV, CD55 or double staining in the lining layer. (i) PCR results: top, collagen type IV mRNA expression in fibroblast-like synoviocytes from 10 RA patients and 11 healthy controls; bottom, β2-microglobulin (β2M) mRNA expression in the same samples. |
PMC1794508_F3_9405.jpg | What is the central feature of this picture? | Collagen type IV staining in healthy and arthritic synovial tissue. Collagen type IV was found in the lining layer of synovial biopsies from (a) healthy human subjects and (c) rheumatoid arthritis (RA) patients. Relative isotype controls are shown in (b,d). Double staining was performed to reveal the cellular origin of the type IV collagen. (e) Double staining was found in the lining layer. For a clearer picture, succeeding slices were stained with (f) CD55 or (g) collagen type IV. (h) Isotype control. Arrows indicate collagen IV, CD55 or double staining in the lining layer. (i) PCR results: top, collagen type IV mRNA expression in fibroblast-like synoviocytes from 10 RA patients and 11 healthy controls; bottom, β2-microglobulin (β2M) mRNA expression in the same samples. |
PMC1794508_F3_9408.jpg | What is the core subject represented in this visual? | Collagen type IV staining in healthy and arthritic synovial tissue. Collagen type IV was found in the lining layer of synovial biopsies from (a) healthy human subjects and (c) rheumatoid arthritis (RA) patients. Relative isotype controls are shown in (b,d). Double staining was performed to reveal the cellular origin of the type IV collagen. (e) Double staining was found in the lining layer. For a clearer picture, succeeding slices were stained with (f) CD55 or (g) collagen type IV. (h) Isotype control. Arrows indicate collagen IV, CD55 or double staining in the lining layer. (i) PCR results: top, collagen type IV mRNA expression in fibroblast-like synoviocytes from 10 RA patients and 11 healthy controls; bottom, β2-microglobulin (β2M) mRNA expression in the same samples. |
PMC1794508_F3_9410.jpg | What stands out most in this visual? | Collagen type IV staining in healthy and arthritic synovial tissue. Collagen type IV was found in the lining layer of synovial biopsies from (a) healthy human subjects and (c) rheumatoid arthritis (RA) patients. Relative isotype controls are shown in (b,d). Double staining was performed to reveal the cellular origin of the type IV collagen. (e) Double staining was found in the lining layer. For a clearer picture, succeeding slices were stained with (f) CD55 or (g) collagen type IV. (h) Isotype control. Arrows indicate collagen IV, CD55 or double staining in the lining layer. (i) PCR results: top, collagen type IV mRNA expression in fibroblast-like synoviocytes from 10 RA patients and 11 healthy controls; bottom, β2-microglobulin (β2M) mRNA expression in the same samples. |
PMC1794508_F3_9404.jpg | What does this image primarily show? | Collagen type IV staining in healthy and arthritic synovial tissue. Collagen type IV was found in the lining layer of synovial biopsies from (a) healthy human subjects and (c) rheumatoid arthritis (RA) patients. Relative isotype controls are shown in (b,d). Double staining was performed to reveal the cellular origin of the type IV collagen. (e) Double staining was found in the lining layer. For a clearer picture, succeeding slices were stained with (f) CD55 or (g) collagen type IV. (h) Isotype control. Arrows indicate collagen IV, CD55 or double staining in the lining layer. (i) PCR results: top, collagen type IV mRNA expression in fibroblast-like synoviocytes from 10 RA patients and 11 healthy controls; bottom, β2-microglobulin (β2M) mRNA expression in the same samples. |
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