Dataset
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Experiment
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Dataset DOI / URL
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2D images
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5.2k
3D acquisitions
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358
Training tiles
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17.6k
Subcellular connectomic analyses of energy networks in striated muscle
Mouse Striated Muscle Mitochondrial Network FIB-SEM
https://zenodo.org/records/3936061
Public
0
3
118
HuaLab Mouse Testis Tissue
Mouse Testis SBF-SEM
https://doi.org/10.6019/EMPIAR-12535
Public
0
1
33
Mouse thymus FIB-SEM volume (jrc_mus-thymus-2a)
Mouse Thymus FIB-SEM
https://openorganelle.janelia.org/datasets/jrc_mus-thymus-2a
Public
0
1
0
ATUM-Tomo: A multi-scale approach to cellular ultrastructure by combined volume scanning electron microscopy and electron tomography
Mouse Traumatic Brain Injury Cortex Correlative ATUM-Tomo
https://doi.org/10.6019/S-BIAD1274
Public
0
6
1,259
Venous puncture wound thrombi, 1, 5, 20 min post-puncture, 100 nm XY raw images, 20 nm XY pixels every 20 micons, 3 nm wide area TEM montages at selected depth
Mouse Venous Puncture Thrombus SBF-SEM
https://doi.org/10.6019/EMPIAR-10785
Public
0
18
3,820
Mouse V1 ssTEM Volume
Mouse Visual Cortex Connectomics TEM
https://webknossos.org/datasets/lee16-5c61b71c0100003f3b177de3/view
Public
0
1
400
Mouse dLGN ATUM-SEM Volume - WEBKNOSSOS
Mouse Visual Thalamus (dLGN) Serial-Section SEM
https://webknossos.org/datasets/morgan2020-6373490b01000026011a53f7/view
Public
0
1
400
Mouse dLGN Serial-Section EM Volume - BossDB
Mouse Visual Thalamus (dLGN) Serial-Section SEM
https://doi.org/10.60533/BOSS-2020-OMCN
Public
0
1
400
NIH-3T3 Control & OPA1-KD FIB-SEM Volumes
NIH-3T3 Mitochondrial Cristae Control & OPA1 Knockdown FIB-SEM
https://doi.org/10.6019/EMPIAR-11449
Public
0
3
226
Nematode pharyngeal connectome ssTEM (BossDB)
Nematode Pharyngeal Connectome TEM
https://doi.org/10.60533/BOSS-2013-XKV2
Public
0
2
593
P. pacificus pharynx ssTEM volume (webKnossos)
Nematode Pharyngeal Connectome TEM
https://webknossos.org/datasets/bumbarger13-5ca4dd07010000a02931d708/view
Public
0
1
611
Focused Ion Beam - Scanning Electron Microscopy volume of the aboral pole of a Nematostella Vectensis polyp
Nematostella vectensis Aboral Pole FIB-SEM
https://doi.org/10.6019/EMPIAR-13099
Public
0
1
72
Nocodazole-Treated HeLa Cell FIB-SEM Volume (jrc_hela-nz-1)
Nocodazole-Treated HeLa Cell FIB-SEM
https://doi.org/10.25378/janelia.24944784
Public
0
1
400
Nocodazole-Treated HeLa Cell FIB-SEM Volume (jrc_hela-nz-2)
Nocodazole-Treated HeLa Cell FIB-SEM (jrc_hela-nz-2)
https://openorganelle.janelia.org/datasets/jrc_hela-nz-2
Public
0
1
400
3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM. - Juvenile
Oikopleura dioica SBF-SEM
https://doi.org/10.5061/dryad.d51c5b012
Public
0
1
400
Serial section images of Oikopleura dioica larva using SBF-SEM - Larva
Oikopleura dioica SBF-SEM
https://doi.org/10.5061/dryad.dbrv15dzh
Public
0
1
400
P7 Mouse Heart FIB-SEM Volume
P7 Mouse Heart FIB-SEM
https://doi.org/10.25378/janelia.24020919
Public
0
1
399
P7 Mouse Hippocampus FIB-SEM Volume
P7 Mouse Hippocampus FIB-SEM
https://doi.org/10.25378/janelia.24085176
Public
0
1
400
P7 Mouse Kidney
P7 Mouse Kidney FIB-SEM
https://doi.org/10.25378/janelia.24020799
Public
0
1
400
P7 Mouse Liver
P7 Mouse Liver FIB-SEM
https://doi.org/10.25378/janelia.24051189
Public
0
1
400
P7 Mouse Pancreas
P7 Mouse Pancreas FIB-SEM
https://doi.org/10.25378/janelia.23411843
Public
0
1
400
P7 Mouse Skin
P7 Mouse Skin FIB-SEM
https://doi.org/10.25378/janelia.24085269
Public
0
1
400
P7 Mouse Thymus
P7 Mouse Thymus FIB-SEM
https://doi.org/10.25378/janelia.24085287
Public
0
1
400
Paramecium Macronucleus TEM
Paramecium Macronucleus TEM
https://doi.org/10.6075/j0nz86k4
Public
1
0
4
Cytoklepty in the plankton: a host strategy to optimize the bioenergetic machinery of endosymbiotic algae
Phaeocystis-Acantharia Photosymbiosis FIB-SEM
https://doi.org/10.6019/EMPIAR-10672
Public
0
8
48
Data from: Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis
Physcomitrium patens Spermatid TEM
https://doi.org/10.5281/zenodo.6546077
Public
0
3
6
FIB-SEM of Gemmata obscuriglobus - a species of the Planctomycetes phylum
Planctomycetes Bacteria FIB-SEM
https://doi.org/10.6019/EMPIAR-10553
Public
0
1
0
Tuwongella immobilis FIB-SEM Volume
Planctomycetes Bacteria FIB-SEM
https://doi.org/10.6019/EMPIAR-10554
Public
0
1
100
Plantorganelle Hunter EM images
Plant Leaf Organelle TEM
https://doi.org/10.57760/sciencedb.01335
Public
929
0
2,459
Potato virus Y coat protein involvement in viral movement
Plant Leaf Potato Virus Y Movement TEM
https://doi.org/10.5281/zenodo.17643798
Public
100
0
385
Plasmodium berghei Male Gamete SBF-SEM
Plasmodium berghei Male Gamete Formation SBF-SEM
https://doi.org/10.6019/EMPIAR-11710
Public
0
2
800
Plasmodium berghei ookinete
Plasmodium berghei Ookinete FIB-SEM
https://doi.org/10.6019/EMPIAR-12956
Public
0
1
24
Cellular Hallmarks from Volume Electron Microscopy reveals Developmental Progression of Plasmodium Ookinetes
Plasmodium berghei Ookinete SBF-SEM
https://doi.org/10.6019/EMPIAR-12951
Public
0
23
146
Comparative 3D ultrastructure of Plasmodium falciparum gametocytes
Plasmodium falciparum Gametocyte 3D Ultrastructure FIB-SEM
https://doi.org/10.6019/EMPIAR-12160
Public
0
24
2,868
SBF-SEM of early schizont-stage malaria parasite infected red blood cell
Plasmodium falciparum-Infected Red Blood Cell SBF-SEM
https://doi.org/10.6019/EMPIAR-10054
Public
0
1
14
SBF-SEM of late schizont-stage malaria parasite infected red blood cell
Plasmodium falciparum-Infected Red Blood Cell SBF-SEM
https://doi.org/10.6019/EMPIAR-10055
Public
0
1
15
SBF-SEM of ring-stage malaria parasite infected red blood cell
Plasmodium falciparum-Infected Red Blood Cell SBF-SEM
https://doi.org/10.6019/EMPIAR-10052
Public
0
1
20
SBF-SEM of trophozoite-stage malaria parasite infected red blood cell
Plasmodium falciparum-Infected Red Blood Cell SBF-SEM
https://doi.org/10.6019/EMPIAR-10053
Public
0
1
19
SBF-SEM datasets of chaetoblasts constructing bristles in Platynereis dumerilii
Platynereis dumerilii Chaetoblast SBF-SEM
https://doi.org/10.6019/EMPIAR-11851
Public
0
3
734
Data from: A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes
Platynereis dumerilii Nerve Cord siGOLD Immunogold TEM
https://doi.org/10.5061/dryad.c7366
Public
12
0
258
FIB-SEM of parapodia from Platynereis dumerilii
Platynereis dumerilii Parapodia FIB-SEM
https://doi.org/10.6019/EMPIAR-10310
Public
0
1
0
Whole-body integration of gene expression and single-cell morphology
Platynereis dumerilii Whole-Body Larva SBF-SEM
https://doi.org/10.6019/EMPIAR-10365
Public
0
1
0
Fib-SEM stacks for Prey (Phaeocystis antarctica) and Host (Ross Sea Dinoflagellate; RSD)
Polar Plankton Organelle Hijacking FIB-SEM
https://doi.org/10.6019/EMPIAR-12627
Public
0
18
1,189
RAEM random-access electron microscopy for revisitable 3D imaging
RAEM Revisitable 3D Imaging SEM
https://doi.org/10.60533/boss-2026-mw96
Public
2
2
354
Rabbit Retina e1088 SBEM Volume
Rabbit Retina SBF-SEM
https://webknossos.org/datasets/e1088_large-5c010a3c010000f6145257c8/view
Public
0
1
211
Denk and Horstmann 2004 rat cortex SBF-SEM
Rat Cerebral Cortex SBF-SEM
https://webknossos.org/datasets/denk2004-5bfd0b1c0100003f3574cc8e
Public
0
1
24
Data for: Axonal Structure-Function relationships across experimental modalities
Rat Corpus Callosum Axon TEM
https://doi.org/10.5281/zenodo.17476529
Public
1
0
58
Harris 2015 Hippocampal Neuropil 3DEM - Apical Dendrite
Rat Hippocampal CA1 Neuropil TEM
https://webknossos.org/datasets/kharris15_apical-5c7e907c010000160cc8d8d0/view
Public
0
1
185
Harris 2015 Hippocampal Neuropil 3DEM - Oblique Dendrite
Rat Hippocampal CA1 Neuropil TEM
https://webknossos.org/datasets/kharris15_oblique-5c7e907c010000790cc8d8cf/view
Public
0
1
85
Harris 2015 Hippocampal Neuropil 3DEM - Spine
Rat Hippocampal CA1 Neuropil TEM
https://webknossos.org/datasets/kharris15_spine-5c7e907c010000c60bc8d8ce/view
Public
0
1
21
Harris 2015 Rat Hippocampal CA1 Neuropil
Rat Hippocampal CA1 Neuropil TEM
https://doi.org/10.60533/BOSS-2015-GYEN
Public
0
3
185
Dl-3-n-butylphthalide alleviates cognitive impairment induced by chronic cerebral hypoperfusion via inhibiting the mitochondrial permeability transition pore opening and excessive mitophagy
Rat Hippocampus Chronic Cerebral Hypoperfusion TEM
https://doi.org/10.5281/zenodo.14253010
Public
9
0
36
GLAM Rat Hippocampus Demo Bundle
Rat Hippocampus FIB-SEM
https://doi.org/10.5061/dryad.808k4r0
Public
0
1
0
Rat Medial Entorhinal Cortex P25 Motortile 1
Rat Medial Entorhinal Cortex SBF-SEM
https://webknossos.org/datasets/HS_st218_MT1-5a7c738a48d7a73cea3c83c2/view
Public
0
1
318
FAST-EM array tomography data of rat pancreas prepared with rOTO protocol and stained with neodymium acetate - EMPIAR-12174 (4x4 mosaics)
Rat Pancreas FAST-EM Array Tomography
https://doi.org/10.6019/EMPIAR-12174
Public
0
43
2,601
FAST-EM array tomography data of rat pancreas prepared with rOTO protocol and stained with neodymium acetate - EMPIAR-12193 (10x10 mosaics)
Rat Pancreas FAST-EM Array Tomography
https://doi.org/10.6019/EMPIAR-12193
Public
0
72
16,889
3D serial block-face scanning electron microscopy (SBF-SEM) imaging of a rat pelvic nerve - Volume 1 (Version 2)
Rat Pelvic Nerve SBF-SEM
https://doi.org/10.26275/8bbl-fzip
Public
0
1
400
SARS-CoV-2-infected Vero Cell FIB-SEM Volume
SARS-CoV-2-Infected Vero Cell FIB-SEM
https://doi.org/10.25378/janelia.16638316
Public
0
1
318
Serial Block Face Scanning Electron Micrscopy dataset of fetal day 64 guinea pig psoas muscle in transverse
SBF-SEM Reconstruction Guide Example Volumes
https://doi.org/10.6019/EMPIAR-10092
Public
0
1
93
Serial Block Face Scanning Electron Micrscopy dataset of the optic lobe from an adult locust
SBF-SEM Reconstruction Guide Example Volumes
https://doi.org/10.6019/EMPIAR-10152
Public
0
1
240
SM WT1 8 nm Lysosome Combined FIB-SEM Volume
SM WT1 Lysosome FIB-SEM
https://webknossos.org/datasets/SM_WT1_8nm_lysosome_combined-661d83c20100008c0298c110/view
Public
0
1
30
Mouse Brown Adipose Tissue WT & Smc5 K371/K371 SBF-SEM
SMC5 Mutant Mouse Brown Adipose Tissue SBF-SEM
https://doi.org/10.6019/EMPIAR-13505
Public
0
2
800
Immortalized breast cancer cell (SUM159) - jrc_sum159-1
SUM159 Breast Cancer Cell FIB-SEM (jrc_sum159-1)
https://doi.org/10.25378/janelia.13114352
Public
0
1
187
Immortalized breast cancer cell (SUM159) - jrc_sum159-4
SUM159 Breast Cancer Cell FIB-SEM (jrc_sum159-4)
https://doi.org/10.25378/janelia.20134103
Public
0
1
400
Adaptive traits of cysts of the snow alga Sanguina nivaloides unveiled by 3D subcellular imaging
Sanguina nivaloides Snow Alga Cyst FIB-SEM
https://doi.org/10.6019/EMPIAR-11694
Public
0
2
7
FIB/SEM sample dataset MCB-CLEM IV Weiner
Shigella flexneri HeLa Cell Invasion FIB-SEM
https://doi.org/10.6019/EMPIAR-10479
Public
0
1
12
Macrophage cell
THP-1 Macrophage FIB-SEM
https://doi.org/10.25378/janelia.13114343
Public
0
1
447
TEM Image Dataset for Mitochondrial Morphology Analysis in Chemotherapy Response and Cancer Metastasis Studies
TNBC Mitochondrial Chemotherapy-Response TEM
https://doi.org/10.5281/zenodo.15608455
Public
1,742
0
13,196
TEM Mitochondria Segmentation Dataset for Triple Negative Breast Cancer Chemotherapy Analysis
TNBC Mitochondrial Chemotherapy-Response TEM
https://doi.org/10.5281/zenodo.15602048
Public
209
0
1,442
Plant SBF-SEM - Tobacco Leaf Chloroplast
Tobacco Leaf Chloroplast SBF-SEM
https://doi.org/10.6019/EMPIAR-11831
Public
0
1
26
Processed FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ΔCAP parasites
Toxoplasma gondii CAP Parasitophorous Vacuole FIB-SEM
https://doi.org/10.6019/EMPIAR-10324
Public
0
1
3
Processed FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ΔCAP parasites, complemented with CAP
Toxoplasma gondii CAP Parasitophorous Vacuole FIB-SEM
https://doi.org/10.6019/EMPIAR-10327
Public
0
1
4
SBF-SEM of the Trichuris muris Stichosome
Trichuris muris Stichosome SBF-SEM
https://doi.org/10.5281/zenodo.3601280
Public
0
2
800
FIB-SEM dataset of a human bone osteosarcoma epithelial cell (U2-OS)
U2-OS Cell FIB-SEM
https://doi.org/10.6019/EMPIAR-11746
Public
0
1
48
FIB-SEM of U2OS cell at leading edge of a wound assay, LPA stimulated, polarized
U2-OS Cell Wound Assay Leading Edge FIB-SEM
https://doi.org/10.6019/EMPIAR-11002
Public
0
1
1
FIB-SEM of U2OS cell at leading edge of a wound assay, non-LPA stimulated, non-polarized
U2-OS Cell Wound-Assay Leading Edge FIB-SEM
https://doi.org/10.6019/EMPIAR-11003
Public
0
1
30
Cryo-FIB-SEM volume (ROI6)
U2OS Cell Mitochondrial Pearling Cryo-FIB-SEM
https://doi.org/10.5281/zenodo.17769238
Public
0
1
400
FIBSEM Run2 Feb 2026 pt1
Unprovenanced FIB-SEM Volume (Run2 Feb 2026 pt1)
https://webknossos.org/datasets/FIBSEM_Run2_Feb2026_pt1-69814e310100009701047c11
Public
0
1
19
Vero Cell Culture Image Dataset
Vero E6 Cell Culture FBS Variation TEM
https://doi.org/10.5281/zenodo.17928456
Public
117
0
1,340
Electron tomography of endosymbiotic bacteria inside a bacteriocyte of the cereal weevil Sitophilus oryzae - EMPIAR-12198
Weevil Bacteriocyte Endosymbiont Tubular Networks ET
https://doi.org/10.6019/EMPIAR-12198
Public
0
1
10
Electron tomography of endosymbiotic bacteria inside a bacteriocyte of the cereal weevil Sitophilus oryzae - EMPIAR-12602
Weevil Bacteriocyte Endosymbiont Tubular Networks ET
https://doi.org/10.6019/EMPIAR-12602
Public
0
1
15
Zebra Finch Brain MagC Correlative Volumes
Zebra Finch Brain MagC Correlative SEM
https://doi.org/10.60533/BOSS-2019-B9NV
Public
0
2
800
Zebra Finch HVC j0256
Zebra Finch HVC SBF-SEM
https://webknossos.org/datasets/j0256-5c051f59010000b538527270/view
Public
0
1
400
Array Tomography of zebrafish axillary lymphoid organ - an external, experimentally accessible immune organ in the zebrafish
Zebrafish Axillary Lymphoid Organ SEM
https://www.ebi.ac.uk/empiar/EMPIAR-12377/
Public
0
7
597
SBF SEM images of a Zebrafish hindbrain containing several Toxoplasma gondii tachizoites at different stages of replication
Zebrafish Hindbrain Toxoplasma Infection
https://doi.org/10.6019/EMPIAR-10463
Public
0
5
1,880
SBF SEM images of a Zebrafish hindbrain macrophage containing 2 Toxoplasma gondii tachizoites
Zebrafish Hindbrain Toxoplasma Infection
https://doi.org/10.6019/EMPIAR-10461
Public
0
3
490
TEM images of a Zebrafish hindbrain cells containing Toxoplasma gondii tachizoites
Zebrafish Hindbrain Toxoplasma Infection
https://doi.org/10.6019/EMPIAR-10460
Public
0
3
180
Zebrafish Hindbrain Macrophage SBF-SEM
Zebrafish Hindbrain Toxoplasma Infection
https://doi.org/10.6019/EMPIAR-10462
Public
0
2
800
Zebrafish Hindbrain ROI01 SBF-SEM
Zebrafish Hindbrain Toxoplasma Infection
https://doi.org/10.6019/EMPIAR-10464
Public
0
11
3,516
Zebrafish Macrophage FIB-SEM Volume
Zebrafish Hindbrain Toxoplasma Infection
https://doi.org/10.6019/EMPIAR-10459
Public
0
1
160
7 day post fertilization larval zebrafish cardiac tissue
Zebrafish Larval Heart FIB-SEM
https://doi.org/10.25378/janelia.22630519
Public
0
1
266
Larval Zebrafish Olfactory Bulb SBEM Volume
Zebrafish Larval Olfactory Bulb SBF-SEM
https://doi.org/10.60533/BOSS-2016-U6PR
Public
0
1
359
Zebrafish lateral-line neuromast SBEM - N1
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_N1-5b4238a61d00004b1d1ab5e2/view
Public
0
1
389
Zebrafish lateral-line neuromast SBEM - N2
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_N2-5b4238a61d0000c42a1ab5e4/view
Public
0
2
658
Zebrafish lateral-line neuromast SBEM - T1
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_T1-5b3ce7bb1d0000340240b91b/view
Public
0
1
231
Zebrafish lateral-line neuromast SBEM - T3
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_T3-5b4238a61d0000c42a1ab5e3/view
Public
0
1
216
Zebrafish lateral-line neuromast SBEM - T4
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_T4-5b4238a61d0000c42a1ab5e5/view
Public
0
1
585
Zebrafish lateral-line neuromast SBEM - WT1
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_WT1-5b3ce7bb1d0000340240b921/view
Public
0
1
388
Zebrafish lateral-line neuromast SBEM - WT2
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_WT2-5b3ce7bb1d0000340240b923/view
Public
0
1
366
Zebrafish lateral-line neuromast SBEM - WT7
Zebrafish Lateral-Line Neuromast SBF-SEM
https://webknossos.org/datasets/Neuromast_WT7-5b3ce7bb1d0000340240b91c/view
Public
0
1
183