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Poxviruses subvert the host immune response by producing immunomodulatory proteins , including a complement regulatory protein . Ectromelia virus provides a mouse model for smallpox where the virus and the host's immune response have co-evolved . Using this model , our study investigated the role of the complement syst...
As one of the most successful pathogens ever , smallpox caused death and disfigurement worldwide until its eradication in the 1970s . The complement system , an essential part of the innate immune response , protects against many pathogens; however , its role during smallpox infection is unclear . In this study , we in...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/virulence", "factors", "and", "mechanisms", "virology/animal", "models", "of", "infection", "immunology/innate", "immunity", "virology/mechanisms", "of", "resistance", "and", "susceptibility,", "including", "host", "genetics", "infectious", "diseases/viral", "infect...
2008
Surviving Mousepox Infection Requires the Complement System
Errors in protein synthesis , so-called phenotypic mutations , are orders-of-magnitude more frequent than genetic mutations . Here , we provide direct evidence that alternative protein forms and phenotypic variability derived from translational errors paved the path to genetic , evolutionary adaptations via gene duplic...
The rarity of genetic mutations limits the likelihood of adaptation . However , transcriptional and translational errors , so-called phenotypic mutations , are >105-fold more frequent , thus generating protein mutants from unmodified genes . We provide the first evidence that phenotypic mutations paved the path to what...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
The Evolutionary Potential of Phenotypic Mutations
The bacterium Helicobacter pylori can cause peptic ulcer disease , gastric adenocarcinoma and MALT lymphoma . The cell-surface mucin MUC1 is a large glycoprotein which is highly expressed on the mucosal surface and limits the density of H . pylori in a murine infection model . We now demonstrate that by using the BabA ...
The bacterium Helicobacter pylori can cause peptic ulcer disease , gastric adenocarcinoma and MALT lymphoma . H . pylori colonize the mucosal surface of the stomach , where adherence helps the bacteria to remain in the neutral and protected niche under the mucus layer , and helps it withstand the continuous mucus washi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/cellular", "microbiology", "and", "pathogenesis", "gastroenterology", "and", "hepatology/gastrointestinal", "cancers", "gastroenterology", "and", "hepatology/gastrointestinal", "infections", "immunology/innate", "immunity", "infectious", "diseases/gastrointestinal", "infe...
2009
MUC1 Limits Helicobacter pylori Infection both by Steric Hindrance and by Acting as a Releasable Decoy
Glial cells surround neuronal endings to create enclosed compartments required for neuronal function . This architecture is seen at excitatory synapses and at sensory neuron receptive endings . Despite the prevalence and importance of these compartments , how they form is not known . We used the main sensory organ of C...
The nervous system of most animals consists of two related cell types , neurons and glia . A striking property of glia is their ability to ensheath neuronal cells , which can help increase the efficiency of synaptic communication between neurons . Sensory neuron receptive endings in the periphery , as well as excitator...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "developmental", "neuroscience", "biology", "sensory", "systems", "morphogenesis", "neuroscience" ]
2011
Opposing Activities of LIT-1/NLK and DAF-6/Patched-Related Direct Sensory Compartment Morphogenesis in C. elegans
Replication Protein A ( RPA ) , the major single stranded DNA binding protein in eukaryotes , is composed of three subunits and is a fundamental player in DNA metabolism , participating in replication , transcription , repair , and the DNA damage response . In human pathogenic trypanosomatids , only limited studies hav...
Trypanosoma cruzi is the etiological agent of Chagas disease . During its life cycle , this parasite alternates between proliferative/non-infective forms and forms that are infective but not able to proliferate . Some stressors , such as acidic pH and starvation , trigger the transition from one form to another; howeve...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "ustilago", "maydis", "crystal", "structure", "condensed", "matter", "physics", "dna-binding", "proteins", "microbiology", "parasitic", "protozoans", "protozoan", "life", "cycles", "cell", "differentiation", "dna", "damage", "developmental", "biology", "fungi", "model", ...
2016
Replication Protein A Presents Canonical Functions and Is Also Involved in the Differentiation Capacity of Trypanosoma cruzi
Cutaneous beta-papillomaviruses are associated with non-melanoma skin cancers that arise in patients who suffer from a rare genetic disorder , Epidermodysplasia verruciformis ( EV ) or after immunosuppression following organ transplantation . Recent studies have shown that the E6 proteins of the cancer associated beta ...
The lack of a genetically tractable small animal model to study viral infection and pathogenesis has significantly hindered papillomavirus research . The recent discovery of Mus musculus papillomavirus 1 ( MmuPV1 ) , which can replicate and form skin warts and cancers in experimentally infected laboratory mouse strains...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "urology", "keratinocytes", "medicine", "and", "health", "sciences", "cancers", "and", "neoplasms", "notch", "signaling", "epithelial", "cells", "oncology", "signal", "inhibition", "immunoprecipitation", "sexually", "transmitted", "diseases", "research", "and", "analysis"...
2017
Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-β Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation
Since 2009 , Fundación Mundo Sano has implemented an Aedes aegypti Surveillance and Control Program in Tartagal city ( Salta Province , Argentina ) . The purpose of this study was to analyze temporal dynamics of Ae . aegypti breeding sites spatial distribution , during five years of samplings , and the effect of contro...
As reported in Porcasi et al . , in Argentina we are working on an integrated risk stratification system based in geospatial technologies that have moderately consolidated national scale , but need more understanding of its urban scale mechanisms . In this work , relevant results are shown on how Ae . aegypti breeding ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "demography", "geographical", "locations", "argentina", "social", "sciences", "animals", "animal", "behavior", "infectious", "disease", "control", "insect", "vectors", "zoology", "housing", "human", "geography", ...
2016
Temporal Dynamics and Spatial Patterns of Aedes aegypti Breeding Sites, in the Context of a Dengue Control Program in Tartagal (Salta Province, Argentina)
Trichinellosis is a typical food-borne zoonotic disease which is epidemic worldwide and the nematode Trichinella spiralis is the main pathogen . The life cycle of T . spiralis contains three developmental stages , i . e . adult worms , new borne larva ( new borne L1 larva ) and muscular larva ( infective L1 larva ) . S...
Trichinellosis of human and other mammals was caused through the ingestion of the parasite Trichinella sparilis in contaminated meat . It is a typical zoonotic disease that affects more than 10 million people world-wide . Parasites of the genus Trichinella are unique intracellular pathogens . Adult Trichinella parasite...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "veterinary", "diseases", "zoonotic", "diseases", "trichinellosis", "parasitic", "diseases", "veterinary", "science", "foodborne", "diseases" ]
2012
Global Gene Expression Analysis of the Zoonotic Parasite Trichinella spiralis Revealed Novel Genes in Host Parasite Interaction
Scrub typhus is a vector-borne zoonotic disease that can be life-threatening . There are no licensed vaccines , or vector control efforts in place . Despite increasing awareness in endemic regions , the public health burden and global distribution of scrub typhus remains poorly known . We systematically reviewed all li...
Scrub typhus is a mite-transmitted infectious disease that can be life-threatening . Diagnosing this disease is difficult , requiring special techniques that are often not readily available . As the actual impact of scrub typhus on the population and its geographical distribution remains unknown , we searched systemati...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "death", "rates", "typhus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "clinical", "research", "design", "geographical", "locations", "india", "bacterial", "diseases", "research", "design", "signs", "and", "symptoms", "case"...
2017
Estimating the burden of scrub typhus: A systematic review
Natural sounds convey perceptually relevant information over multiple timescales , and the necessary extraction of multi-timescale information requires the auditory system to work over distinct ranges . The simplest hypothesis suggests that temporal modulations are encoded in an equivalent manner within a reasonable in...
Correctly perceiving behaviorally significant sounds—speech , music , and the acoustic environment—requires integrating acoustic information over time to extract relevant regularities . A fundamental question about this process is: How does the auditory brain integrate information of continuously varying sounds , typic...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "acoustics", "medicine", "and", "health", "sciences", "linguistics", "engineering", "and", "technology", "signal", "processing", "social", "sciences", "neuroscience", "brain", "mapping", "neuroimaging", "research", "and", "analysis", "methods", "sensory", "physiology", ...
2017
Concurrent temporal channels for auditory processing: Oscillatory neural entrainment reveals segregation of function at different scales
Elongator is a conserved protein complex comprising six different polypeptides that has been ascribed a wide range of functions , but which is now known to be required for modification of uridine residues in the wobble position of a subset of tRNAs in yeast , plants , worms and mammals . In previous work , we showed th...
tRNA molecules function as adapters in protein synthesis , bringing amino acids to the ribosome and reading the genetic code through codon-anticodon base pairing . When the tRNA contains a uridine residue in the “wobble position” of its anticodon , which base-pairs with purine residues in the third position of a cognat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "phosphorylation", "transfer", "rna", "spectrometric", "identification", "of", "proteins", "molecular", "complexes", "fungal", "genetics", "nucleotides", "anticodons", "molecular", "genetics", "microbial", "genetics", "proteins", "proteomics", "molecular", "biology", "uridi...
2015
Phosphorylation of Elp1 by Hrr25 Is Required for Elongator-Dependent tRNA Modification in Yeast
Plasmodium vivax malaria has a wide geographic distribution and poses challenges to malaria elimination that are likely to be greater than those of P . falciparum . Diagnostic tools for P . vivax infection in non-reference laboratory settings are limited to microscopy and rapid diagnostic tests but these are unreliable...
Plasmodium vivax has a worldwide distribution and is the second most common causative agent of human malaria . The dormant liver stage of P . vivax allows the infection to recur unless diagnosed and treated appropriately , which poses a significant challenge to the goals of malaria elimination and eradication as outlin...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "parasite", "groups", "medicine", "and", "health", "sciences", "body", "fluids", "plasmodium", "engineering", "and", "technology", "laboratory", "equipment", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "parasitology", "parasitemia", "organi...
2016
Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination
ChIP-based genome-wide assays of transcription factor ( TF ) occupancy have emerged as a powerful , high-throughput method to understand transcriptional regulation , especially on a global scale . This has led to great interest in the underlying biochemical mechanisms that direct TF-DNA binding , with the ultimate goal...
Chromatin Immunoprecipitation ( ChIP ) -based genome-wide assays of transcription factor ( TF ) occupancy have emerged as a powerful , high throughput method to understand transcriptional regulation , especially on a global scale . Here , we utilize 45 ChIP-chip and ChIP-SEQ data sets from Drosophila to explore the und...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "sequence", "analysis", "systems", "biology", "functional", "genomics", "biophysic", "al", "simulations", "regulatory", "networks", "biology", "genomics", "computational", "biology" ]
2013
Computational Identification of Diverse Mechanisms Underlying Transcription Factor-DNA Occupancy
Gene expression is generally regulated by recruitment of transcription factors and RNA polymerase II ( RNAP II ) to specific sequences in the gene promoter region . The Integrator complex mediates processing of small nuclear RNAs ( snRNAs ) as well as the initiation and release of paused RNAP II at specific genes in re...
The gene transcription profile determines the developmental state of an organism . During embryogenesis , aging , starvation or any lifecycle stage , organisms express specific sets of genes that must be turned off at other stages to maintain the correct metabolic and differentiated state of the cells . Mutations that ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "rna", "interference", "engineering", "and", "technology", "electronics", "caenorhabditis", "gene", "regulation", "integrators", "animals", "dna", "transcription", "small", "nuclear", "rna", "animal", "models", "caenorhabditis", "elegans", "model", "organ...
2019
Disruption of the Caenorhabditis elegans Integrator complex triggers a non-conventional transcriptional mechanism beyond snRNA genes
Between August 2012 and April 2013 the Career Development Fellowship programme of the Special Programme for Research and Training in Tropical Diseases ( World Health Organization ) underwent an external evaluation to assess its past performance and determine recommendations for future programme development and continuo...
The asymmetry of research training between high and low and middle income countries ( LMICs ) and the resulting need for research capacity strengthening ( RCS ) in under-resourced regions has long been established . In 1999 , the World Health Organization ( WHO ) , through the Special Programme for Research and Trainin...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "graduates", "employment", "medicine", "and", "health", "sciences", "alumni", "social", "sciences", "careers", "research", "design", "clinical", "medicine", "global", "health", "surveys", "pharmacology", "educational", "status", "medical", "ethics", "science", "and", ...
2016
Research Capacity Strengthening in Low and Middle Income Countries – An Evaluation of the WHO/TDR Career Development Fellowship Programme
The Drosophila testis is a well-established system for studying stem cell self-renewal and competition . In this tissue , the niche supports two stem cell populations , germ line stem cells ( GSCs ) , which give rise to sperm , and somatic stem cells called cyst stem cells ( CySCs ) , which support GSCs and their desce...
Niches are specialized local environments that support stem cell self-renewal through the local production of short-range signals . In many tissues , resident stem cells compete with each other for niche access . Stem cells that receive multiple self-renewal cues have to integrate these discrete signals to prevent exce...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "reproductive", "system", "rna", "interference", "cloning", "cell", "differentiation", "animal", "signaling", "and", "communication", "developmental", "biology", "animal", "behavior", "stem", "cells", "molecular", "biology", "tec...
2016
Socs36E Controls Niche Competition by Repressing MAPK Signaling in the Drosophila Testis
It is estimated that a large proportion of amino acid substitutions in Drosophila have been fixed by natural selection , and as organisms are faced with an ever-changing array of pathogens and parasites to which they must adapt , we have investigated the role of parasite-mediated selection as a likely cause . To quanti...
All organisms are attacked by an ever-changing array of pathogens and parasites , and it is widely supposed that the ensuing host–parasite “arms race” must drive extensive adaptive evolution in genes of the immune system . Here we have taken advantage of new sequencing technologies and analytical approaches to quantify...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "genetics", "and", "genomics/population", "genetics", "genetics", "and", "genomics/genetics", "of", "the", "immune", "system", "evolutionary", "biology/genomics" ]
2009
Quantifying Adaptive Evolution in the Drosophila Immune System
West Nile virus ( WNV ) , a zoonotic pathogen naturally transmitted by mosquitoes whose natural hosts are birds , has spread worldwide during the last few decades . Resident birds play an important role in flavivirus epidemiology , since they can serve as reservoirs and facilitate overwintering of the virus . Herein , ...
Birds play an important role in the epidemiology of flaviviruses such as West Nile virus ( WNV ) since birds are natural hosts and facilitate hibernation of the virus in periods of absence of mosquitoes that transmit the virus . Since it has been proposed that magpies play an important role in an endemic WNV cycle in h...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "geographical", "locations", "microbiology", "vertebrates", "animals", "feathers", "viruses", "animal", "anatomy", "r...
2018
High susceptibility of magpie (Pica pica) to experimental infection with lineage 1 and 2 West Nile virus
Detection and sequencing of chikungunya virus ( CHIKV ) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification ( RT-LAMP ) method and a MinION sequencer . We developed the protocol for drying all the reagents for the RT-LAMP in a single reaction tube . Using ...
Chikungunya virus has re-emerged as an important pathogen causing several outbreaks in the world . As the clinical symptoms of chikungunya is similar to other mosquito-borne febrile diseases , the definitive diagnosis of the disease is based on the detection of viral genome from the patient blood . Loop-mediated isothe...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "togaviruses", "chikungunya", "infection", "pathogens", "tropical", "diseases", "microbiology", "alphaviruses", "viruses", "rna", "amplification", "chikungunya", "viru...
2019
Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing
Cell cycle control must be modified at meiosis to allow two divisions to follow a single round of DNA replication , resulting in ploidy reduction . The mechanisms that ensure meiosis termination at the end of the second and not at the end of first division are poorly understood . We show here that Arabidopsis thaliana ...
Meiosis is a fundamental process for sexually reproducing organisms that creates genetic diversity within populations . A key feature of meiosis is the reduction of the number of chromosomes , from two sets to one set , prior to fertilization . This reduction in chromosome number is due to two cell divisions following ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "phosphorylation", "meiosis", "biochemistry", "peptides", "cell", "biology", "chromosome", "biology", "proteins", "post-translational", "modification", "anaphase", "metaphase", "cell", "cycle", "and", "cell", "division", "biology", "and", "life", "sciences", "cell", "pr...
2016
TDM1 Regulation Determines the Number of Meiotic Divisions
A fundamental property of cell populations is their growth rate as well as the time needed for cell division and its variance . The eukaryotic cell cycle progresses in an ordered sequence through the phases and and is regulated by environmental cues and by intracellular checkpoints . Reflecting this regulatory complexi...
Among the important characteristics of dividing cell populations is the time necessary for cells to complete each of the cell cycle phases , that is , to increase the cell's mass , to duplicate and repair its genome , to properly segregate its chromosomes , and to make decisions whether to continue dividing or enter a ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "flow", "cytometry", "systems", "biology", "cytophotometry", "cell", "biology", "theoretical", "biology", "cell", "cycle", "and", "cell", "division", "biology", "and", "life", "sciences", "cell", "processes", "cytometry", "molecular", "cell", "biology", "computational...
2014
Quantifying the Length and Variance of the Eukaryotic Cell Cycle Phases by a Stochastic Model and Dual Nucleoside Pulse Labelling
The Epstein-Barr virus ( EBV ) nuclear proteins EBNA3A , EBNA3B , and EBNA3C interact with the cell DNA binding protein RBPJ and regulate cell and viral genes . Repression of the CDKN2A tumor suppressor gene products p16INK4A and p14ARF by EBNA3A and EBNA3C is critical for EBV mediated transformation of resting B lymph...
Epstein-Barr virus ( EBV ) is a gammaherpesvirus implicated in the pathogenesis of multiple malignancies , including Burkitt lymphoma , Hodgkin lymphoma , post-transplant lymphoproliferative disease ( PTLD ) , nasopharyngeal carcinoma , and gastric carcinoma . EBV infection of resting B-lymphocytes drives them to proli...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
The EBNA3 Family of Epstein-Barr Virus Nuclear Proteins Associates with the USP46/USP12 Deubiquitination Complexes to Regulate Lymphoblastoid Cell Line Growth
Orientia tsutsugamushi is an intracellular α-proteobacterium which resides in trombiculid mites , and is the causative agent of scrub typhus in East Asia . The genome sequence of this species has revealed an unprecedented number of repeat sequences , most notably of the genes encoding the conjugative properties of a ty...
Scrub typhus , the rickettsial infectious disease caused by the obligate intracellular bacterium Orientia tsutsugamushi , is endemic across the Asia Pacific region . The bacterium is transmitted by the bite of larval stages of trombiculid mites ( “chiggers”; Leptotrombidium spp . ) , which more typically feed on small ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "infectious", "diseases/bacterial", "infections", "infectious", "diseases/epidemiology", "and", "control", "of", "infectious", "diseases" ]
2010
High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
When planning a series of actions , it is usually infeasible to consider all potential future sequences; instead , one must prune the decision tree . Provably optimal pruning is , however , still computationally ruinous and the specific approximations humans employ remain unknown . We designed a new sequential reinforc...
Planning is tricky because choices we make now affect future choices , and future choices and outcomes should guide current choices . Because there are exponentially many combinations of future choices and actions , brute-force approaches that consider all possible combinations work only for trivially small problems . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cognitive", "neuroscience", "biology", "neuroscience" ]
2012
Bonsai Trees in Your Head: How the Pavlovian System Sculpts Goal-Directed Choices by Pruning Decision Trees
Since the first recorded epidemic of syphilis in 1495 , controversy has surrounded the origins of the bacterium Treponema pallidum subsp . pallidum and its relationship to the pathogens responsible for the other treponemal diseases: yaws , endemic syphilis , and pinta . Some researchers have argued that the syphilis-ca...
For 500 years , controversy has raged around the origin of T . pallidum subsp . pallidum , the bacterium responsible for syphilis . Did Christopher Columbus and his men introduce this pathogen into Renaissance Europe , after contracting it during their voyage to the New World ? Or does syphilis have a much older histor...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/microbial", "evolution", "and", "genomics", "evolutionary", "biology/microbial", "evolution", "and", "genomics", "infectious", "diseases/sexually", "transmitted", "diseases", "evolutionary", "biology/evolutionary", "and", "comparative", "genetics", ...
2008
On the Origin of the Treponematoses: A Phylogenetic Approach
Anthelmintic resistance is a major problem for the control of parasitic nematodes of livestock and of growing concern for human parasite control . However , there is little understanding of how resistance arises and spreads or of the “genetic signature” of selection for this group of important pathogens . We have inves...
Parasitic nematodes ( roundworms ) are major causes of disease in both domestic animals and humans . Strategic treatments with anthelmintic drugs have been used to control livestock parasites for several decades resulting in widespread drug resistance . Drug treatments have , until recently , been applied at a relative...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
The Emergence of Resistance to the Benzimidazole Anthlemintics in Parasitic Nematodes of Livestock Is Characterised by Multiple Independent Hard and Soft Selective Sweeps
African trypanosomes are mammalian pathogens that must regularly change their protein coat to survive in the host bloodstream . Chronic trypanosome infections are potentiated by their ability to access a deep genomic repertoire of Variant Surface Glycoprotein ( VSG ) genes and switch from the expression of one VSG to a...
Chromosomal translocations can fuel genetic change or cause catastrophic genomic damage . African trypanosomes , exemplified by Trypanosoma brucei sub-species , are unicellular parasites that can chronically infect their human and livestock hosts by using a strategy of antigenic variation by which they repeatedly chang...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "sequencing", "techniques", "cell", "cycle", "and", "cell", "division", "cell", "processes", "cloning", "parasitic", "protozoans", "protozoans", "archives", "sequence", "motif", "analysis", "molecular", "biology", "techniques", "dna", "dna", "recombination", "gene", "...
2016
A Conserved DNA Repeat Promotes Selection of a Diverse Repertoire of Trypanosoma brucei Surface Antigens from the Genomic Archive
T-killer cells of the immune system eliminate virus-infected and tumorous cells through direct cell–cell interactions . Reorientation of the killing apparatus inside the T cell to the T-cell interface with the target cell ensures specificity of the immune response . The killing apparatus can also oscillate next to the ...
Beyond the more widely known molecular recognition of antigen , specificity of the cellular immune response relies on the precise orientation of immune cells toward infected and tumorous cells . We studied the mechanics of the structural orientation of T-killer cells ( a type of immune cells ) to their immunological ta...
[ "Abstract", "Introduction", "Results/Discussion" ]
[ "cell", "biology/morphogenesis", "and", "cell", "biology", "biophysics/theory", "and", "simulation", "immunology", "computational", "biology", "cell", "biology/cytoskeleton" ]
2009
Deterministic Mechanical Model of T-Killer Cell Polarization Reproduces the Wandering of Aim between Simultaneously Engaged Targets
In chronic infections , pathogens are often in the presence of other microbial species . For example , Pseudomonas aeruginosa is a common and detrimental lung pathogen in individuals with cystic fibrosis ( CF ) and co-infections with Candida albicans are common . Here , we show that P . aeruginosa biofilm formation and...
In many human infections , several species of microbes are often present . This is typically the case with the disease cystic fibrosis , characterized by thick mucus in the lungs that is colonized by bacteria and fungi . Here , we show evidence that interactions between the bacterium Pseudomonas aeruginosa and the fung...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "biology", "and", "life", "sciences", "mycology", "microbiology" ]
2014
Candida albicans Ethanol Stimulates Pseudomonas aeruginosa WspR-Controlled Biofilm Formation as Part of a Cyclic Relationship Involving Phenazines
The genetic dissection of the phenotypes associated with Williams-Beuren Syndrome ( WBS ) is advancing thanks to the study of individuals carrying typical or atypical structural rearrangements , as well as in vitro and animal studies . However , little is known about the global dysregulations caused by the WBS deletion...
A fundamental question in current biomedical research is to establish a link between genomic variation and phenotypic differences , which encompasses both the seemingly neutral diversity , as well as the pathological variation that causes or predisposes to disease . Once the primary genetic cause ( s ) of a disease or ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "computational", "biology/transcriptional", "regulation", "genetics", "and", "genomics/gene", "expression", "genetics", "and", "genomics/bioinformatics", "computational", "biology/genomics", "computational", "biology", "genetics", "and", ...
2011
Using Transcription Modules to Identify Expression Clusters Perturbed in Williams-Beuren Syndrome
Enterohemorrhagic Escherichia coli ( EHEC ) , particularly serotype O157:H7 , causes hemorrhagic colitis , hemolytic uremic syndrome , and even death . In vitro studies showed that Shiga toxin 2 ( Stx2 ) , the primary virulence factor expressed by EDL933 ( an O157:H7 strain ) , is encoded by the 933W prophage . And the...
Infection with Enterohemorrhagic E . coli ( EHEC ) , and more recently with the Enteroaggregative E . coli strain O104:H4 , is a significant health risk , causing bloody diarrhea , kidney failure , and even death . The virulence factor in these bacteria responsible for the severe outcomes is Shiga toxin ( Stx ) . Genes...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "microbiology" ]
2013
Prophage Induction Is Enhanced and Required for Renal Disease and Lethality in an EHEC Mouse Model
Epigenetic changes are widely considered to play an important role in aging , but experimental evidence to support this hypothesis has been scarce . We have used array-based analysis to determine genome-scale DNA methylation patterns from human skin samples and to investigate the effects of aging , chronic sun exposure...
Although a role of epigenetic mechanisms in aging and in the adaptation to environmental exposures has been widely assumed , research in this area has been hampered by major methodological challenges . We have now used a novel platform for genome-scale methylation analysis to determine the methylation patterns of human...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/epigenetics", "dermatology/photodermatology", "and", "skin", "aging", "developmental", "biology/aging", "molecular", "biology/dna", "methylation" ]
2010
Aging and Chronic Sun Exposure Cause Distinct Epigenetic Changes in Human Skin
Leptospirosis is a major cause of febrile illness in Africa but little is known about risk factors for human infection . We conducted a cross-sectional study to investigate risk factors for acute leptospirosis and Leptospira seropositivity among patients with fever attending referral hospitals in northern Tanzania . We...
Leptospirosis is an under-recognized but important cause of febrile illness and death in Africa . The bacteria that cause leptospirosis have their usual life cycle in animals; humans are infected as accidental hosts . There is considerable variation between countries as to which reservoir animals and human activities a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "livestock", "medicine", "and", "health", "sciences", "surface", "water", "leptospira", "body", "fluids", "pathology", "and", "laboratory", "medicine", "ruminants", "pathogens", "tropical", "diseases", "microbiology", "vertebrates", "animals", "mammals", "urine", "bacte...
2018
Risk factors for human acute leptospirosis in northern Tanzania
HIV-1 is dependent on the host cell for providing the metabolic resources for completion of its viral replication cycle . Thus , HIV-1 replicates efficiently only in activated CD4+ T cells . Barriers preventing HIV-1 replication in resting CD4+ T cells include a block that limits reverse transcription and also the lack...
HIV-1 is controlled by host restriction factors that interfere with its life cycle . However , the virus has equipped itself to counter these strategies . We report a new interplay between HIV-1 and human T lymphocytes through the FOXO1 transcription factor . By using AS1842856 , a drug targeting FOXO1 , we found that ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "phosphorylation", "cell", "physiology", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "cell", "cycle", "and", "cell", "division", "immunology", "cell", "processes", "microbiolo...
2019
FOXO1 transcription factor plays a key role in T cell—HIV-1 interaction
Genome-wide association studies ( GWAS ) have identified chromosomal loci that affect risk of coronary heart disease ( CHD ) independent of classical risk factors . One such association signal has been identified at 6q23 . 2 in both Caucasians and East Asians . The lead CHD-associated polymorphism in this region , rs12...
Both genetic and environmental factors cumulatively contribute to coronary heart disease risk in human populations . Large-scale meta-analyses of genome-wide association studies have now leveraged common genetic variation to identify multiple sites of disease susceptibility; however , the causal mechanisms for these as...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "coronary", "artery", "disease", "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "functional", "genomics", "rna", "interference", "cardiovascular", "anatomy", "rna", "stability", "gene", "function", "genome", "analysis", "atherosclerosis"...
2014
Coronary Heart Disease-Associated Variation in TCF21 Disrupts a miR-224 Binding Site and miRNA-Mediated Regulation
Many infections can be transmitted between animals and humans . The epidemiological roles of different species can vary from important reservoirs to dead-end hosts . Here , we present a method to identify transmission cycles in different combinations of species from field data . We used this method to synthesise epidem...
Gambiense sleeping sickness is a disease transmitted by tsetse flies that mostly affects rural populations in sub-Saharan Africa . Although the parasite that causes the disease can be found in many different wild and domestic animal species , the disease has often been claimed to be maintained mostly by humans . Curren...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "veterinary", "diseases", "population", "modeling", "african", "trypanosomiasis", "zoonotic", "diseases", "neglected", "tropical", "diseases", "biology", "computational", "biology", "infectious", "disease", "modeling", "parasitic", "dis...
2013
Identifying Transmission Cycles at the Human-Animal Interface: The Role of Animal Reservoirs in Maintaining Gambiense Human African Trypanosomiasis
Sequencing family DNA samples provides an attractive alternative to population based designs to identify rare variants associated with human disease due to the enrichment of causal variants in pedigrees . Previous studies showed that genotype calling accuracy can be improved by modeling family relatedness compared to s...
To identify disease variants that occur less frequently in population , sequencing families in which multiple individuals are affected is more powerful due to the enrichment of causal variants . An important step in such studies is to infer individual genotypes from sequencing data . Existing methods do not utilize ful...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Leveraging Identity-by-Descent for Accurate Genotype Inference in Family Sequencing Data
Faithful DNA replication with correct termination is essential for genome stability and transmission of genetic information . Here we have investigated the potential roles of Topoisomerase II ( Top2 ) and the RecQ helicase Sgs1 during late stages of replication . We find that cells lacking Top2 and Sgs1 ( or Top3 ) dis...
Replication termination is the final step of the replication process , where the two replication forks converge and finally merge to form fully replicated sister chromatids . During this process topological strain in the form of DNA overwinding is generated between forks , and if not removed this strain will inhibit re...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination
Substantial experimental evidence suggests the cerebellum is involved in calibrating sensorimotor maps . Consistent with this involvement is the well-known , but little understood , massive cerebellar projection to maps in the superior colliculus . Map calibration would be a significant new role for the cerebellum give...
The human brain contains a structure known as the cerebellum , which contains a vast number of neurons–around 80% of the total ~90 billion . We believe the cerebellum is involved in learning motor skills , and so is vitally important for accurately controlling the movements of our body , amongst other things . However ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "learning", "medicine", "and", "health", "sciences", "engineering", "and", "technology", "nervous", "system", "signal", "processing", "topographic", "maps", "brain", "social", "sciences", "electrophysiology", "neuroscience", "learning", "and", "memory", "signal", "filte...
2019
Sensorimotor maps can be dynamically calibrated using an adaptive-filter model of the cerebellum
The phenotypic effect of some single nucleotide polymorphisms ( SNPs ) depends on their parental origin . We present a novel approach to detect parent-of-origin effects ( POEs ) in genome-wide genotype data of unrelated individuals . The method exploits increased phenotypic variance in the heterozygous genotype group r...
Large genetic association studies have revealed many genetic factors influencing common traits , such as body mass index ( BMI ) . These studies assume that the effect of genetic variants is the same regardless of whether they are inherited from the mother or the father . In our study , we have developed a new approach...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome-wide", "association", "studies", "developmental", "biology", "genomics", "mathematics", "statistics", "(mathematics)", "genome", "analysis", "genomic", "imprinting", "genetics", "biology", "and", "life", "sciences", "computational", "biology", "biostatistics", "phys...
2014
Novel Approach Identifies SNPs in SLC2A10 and KCNK9 with Evidence for Parent-of-Origin Effect on Body Mass Index
Chromoblastomycosis ( CBM ) is a difficult-to-treat chronic subcutaneous mycosis . In Brazil , the main agent of this disease is Fonsecaea pedrosoi , which is phenotypically very similar to other Fonsecaea species , differing only genetically . The correct species identification is relevant since different species may ...
Chromoblastomycosis is a disfiguring disease usually occurring in rural workers from poor and remote communities . In Brazil , the most frequent agents of this neglected disease are the species belonging to the genus Fonsecaea . The disease occurs after traumatic inoculation during work . As the lesions progress , itch...
[ "Abstract", "Introduction", "Material", "and", "methods", "Results", "Discussion" ]
[ "antimicrobials", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "drugs", "geographical", "locations", "microbiology", "antifungals", "surgical", "and", "invasive", "medical", "procedures", "fungi", "signs", "and", "s...
2018
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil
Striped skunks are one of the most important terrestrial reservoirs of rabies virus in North America , and yet the prevalence of rabies among this host is only passively monitored and the disease among this host remains largely unmanaged . Oral vaccination campaigns have not efficiently targeted striped skunks , while ...
Despite the long recognition that skunks are an important reservoir host for rabies , the control of this disease among this host has not been achieved , and the disease is currently only passively monitored in North America . The need for rabies control among striped skunks is , however , well acknowledged , and repor...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "animal", "types", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "domestic", "animals", "tropical", "diseases", "microbiology", "geographical", "locations", "animals", "...
2016
Bayesian Spatiotemporal Pattern and Eco-climatological Drivers of Striped Skunk Rabies in the North Central Plains
Metabolic changes within the cell and its niche affect cell fate and are involved in many diseases and disorders including cancer and viral infections . Kaposi’s sarcoma-associated herpesvirus ( KSHV ) is the etiological agent of Kaposi’s sarcoma ( KS ) . KSHV latently infected cells express only a subset of viral gene...
The metabolic state within a cell and its local environment is altered in many diseases and disorders including those caused by viral infections . The gamma-herpesviruses Kaposi’s Sarcoma Associated Herpesvirus ( KSHV ) is a viral agent associated with a large number of human malignancies . KSHV was shown to manipulate...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "physiology", "medicine", "and", "health", "sciences", "vesicles", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "metabolic", "processes", "microbiology", "cell", "metabolism", "glycolysis", "viruses", "physiological", "processes...
2017
Herpesviruses shape tumour microenvironment through exosomal transfer of viral microRNAs
Mammalian circadian rhythm is established by the negative feedback loops consisting of a set of clock genes , which lead to the circadian expression of thousands of downstream genes in vivo . As genome-wide transcription is organized under the high-order chromosome structure , it is largely uncharted how circadian gene...
Circadian rhythm regulates daily oscillations of many physiological processes in a wide range of organisms . In mammals , circadian rhythm drives the cycling expression of thousands of downstream genes . The temporal control of transcription takes place under high-order chromosome structure , which is established by lo...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biochemistry", "circadian", "rhythms", "dna", "transcription", "proteins", "transcription", "factors", "gene", "expression", "genetics", "chronobiology", "biology", "and", "life", "sciences", "mammalian", "genomics", "gene", "regulation", "genomics", "dna-binding", "prot...
2016
Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression
This paper discusses the presence of Aedes aegypti pupae in different types of containers considering: volume , pH of the container , among other variables . A nonlinear method for selection was applied , based on Mutual Information , by placing in order of importance the most appropriate variables for identifying cont...
The authors discuss a nonlinear method , based on Mutual Information , for selection of the presence of Aedes aegypti pupae in different breeding sites . The authors compare this method with the logistic regression model . In this study , using the Neural Network model , the variables that better discriminate the conta...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "water", "resources", "pathology", "and", "laboratory", "medicine", "togaviruses", "neural", "networks", "pathogens", "microbiology", "neuroscience", "animals", "alpha...
2018
Classification of containers with Aedes aegypti pupae using a Neural Networks model
Accurate chromosome segregation during meiosis relies on the prior establishment of at least one crossover recombination event between homologous chromosomes . Most meiotic recombination intermediates that give rise to interhomolog crossovers are embedded within a hallmark chromosomal structure called the synaptonemal ...
Reproductive cell formation relies on a nuclear division cycle called meiosis , wherein two homologous sets of chromosomes are reduced to one . At the crux of ( and critically required for ) meiotic chromosome segregation is a transient association between homologous chromosomes established by a crossover recombination...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "meiosis", "cell", "cycle", "and", "cell", "division", "cell", "processes", "meiotic", "prophase", "fungi", "model", "organisms", "sumoylation", "experimental", "organism", "systems", "protein", "structure", "dna", "homologous", "recombination", "research", "and", "an...
2019
Crossover recombination and synapsis are linked by adjacent regions within the N terminus of the Zip1 synaptonemal complex protein
As part of on-going efforts to control hookworm infection , the “human hookworm vaccine initiative” has recognised blood feeding as a feasible therapeutic target for inducing immunity against hookworm infection . To this end , molecular approaches have been used to identify candidate targets , such as Necator americanu...
Hookworm infections ( Necator americanus or Ancylostoma duodenale ) represent a major neglected tropical disease affecting approximately 450 million people worldwide and causing morbidity due to their need to feed on host blood resulting in severe anemia . New chemotherapy and vaccines are needed to combat hookworm inf...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "helminths", "hookworms", "pigments", "animals", "parasitic", "diseases", "toxicology", "developmental", "biology", "materials", "science", "digestive...
2018
A novel blood-feeding detoxification pathway in Nippostrongylus brasiliensis L3 reveals a potential checkpoint for arresting hookworm development
Predicting the effects of mutations on the kinetic rate constants of protein-protein interactions is central to both the modeling of complex diseases and the design of effective peptide drug inhibitors . However , while most studies have concentrated on the determination of association rate constants , dissociation rat...
Within a cell , protein-protein interactions vary considerably in their degree of stickiness . Mutations at protein interfaces can alter the interaction between protein pairs , causing them to dissociate faster or slower . This may lead to an alteration in the dynamics of the cellular networks in which these proteins a...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[]
2013
Characterizing Changes in the Rate of Protein-Protein Dissociation upon Interface Mutation Using Hotspot Energy and Organization
Here , we investigated intrinsic spinal cord mechanisms underlying the physiological requirement for autonomic and somatic motor system coupling . Using an in vitro spinal cord preparation from newborn rat , we demonstrate that the specific activation of muscarinic cholinergic receptors ( mAchRs ) ( with oxotremorine )...
Physical movements require mobilization of animals’ autonomic nervous system , in order to maintain stable bodily functions while matching the increasing physiological demand . These autonomic responses rely on a coupling between the sympathetic and somatic nervous systems , although how this coupling occurs remains un...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "neural", "networks", "nervous", "system", "membrane", "potential", "electrophysiology", "neuroscience", "biological", "locomotion", "serotonin", "muscarinic", "acetylcholine", "receptors", "neurotransmitters", "spina...
2018
Cholinergic-mediated coordination of rhythmic sympathetic and motor activities in the newborn rat spinal cord
Acquisition of adaptive mutations is essential for microbial persistence during chronic infections . This is particularly evident during chronic Pseudomonas aeruginosa lung infections in cystic fibrosis ( CF ) patients . Thus far , mutagenesis has been attributed to the generation of reactive species by polymorphonucle...
Antimicrobial peptides ( AMPs ) are produced by the mammalian immune system to fight invading pathogens . The best understood function of AMPs is to interact with the membranes of microbes , thereby disrupting and killing cells . However , the amount of AMP available during chronic bacterial infections may not be suffi...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences", "microbiology" ]
2014
Cationic Antimicrobial Peptides Promote Microbial Mutagenesis and Pathoadaptation in Chronic Infections
Analogously to chromosome cohesion in eukaryotes , newly replicated DNA in E . coli is held together by inter-sister linkages before partitioning into daughter nucleoids . In both cases , initial joining is apparently mediated by DNA catenation , in which replication-induced positive supercoils diffuse behind the fork ...
Sister chromosome cohesion in eukaryotes maintains genome stability by mediating chromosome segregation and homologous recombination-dependent DNA repair . Here we have investigated the mechanism of cohesion regulation in E . coli by measuring cohesion timing in a broad set of candidate mutant strains . Using a sensiti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "escherichia", "coli", "prokaryotic", "models", "dna", "metabolism", "model", "organisms", "dna", "replication", "chromosome", "biology", "chromosome", "structure", "and", "function", "nucleic", "acids", "dna", "dna", "structure", "biology", "genomics", "molecular", "...
2013
Regulation of Sister Chromosome Cohesion by the Replication Fork Tracking Protein SeqA
Considerable progress has been made in identifying the targets of plant microRNAs , many of which regulate the stability or translation of mRNAs that encode transcription factors involved in development . In most cases , it is unknown , however , which immediate transcriptional targets mediate downstream effects of the...
Short , single-stranded RNA molecules called microRNAs ( miRNAs ) regulate gene expression by negatively controlling both the stability and translation of target messenger RNAs that they recognize through sequence complementarity . In plants , miRNAs mostly regulate other regulators , the DNA-binding transcription fact...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics", "plant", "biology" ]
2008
Control of Jasmonate Biosynthesis and Senescence by miR319 Targets
The serine-rich repeat glycoprotein Srr1 of Streptococcus agalactiae ( GBS ) is thought to be an important adhesin for the pathogenesis of meningitis . Although expression of Srr1 is associated with increased binding to human brain microvascular endothelial cells ( hBMEC ) , the molecular basis for this interaction is ...
Streptococcus agalactiae ( Group B streptococcus , GBS ) is a leading cause of meningitis in newborns and infants . This life-threatening infection of the brain and surrounding tissues continues to result in a high incidence of morbidity and mortality , despite antibiotic therapy . A key factor in disease production is...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "pathogenesis", "streptococci", "emerging", "infectious", "diseases", "microbial", "pathogens", "biology", "microbiology", "host-pathogen", "interaction", "bacterial", "pathogens", "gram", "positive" ]
2012
Binding of Glycoprotein Srr1 of Streptococcus agalactiae to Fibrinogen Promotes Attachment to Brain Endothelium and the Development of Meningitis
Rickettsiosis is a re-emergent infectious disease without epidemiological surveillance in Colombia . This disease is generally undiagnosed and several deadly outbreaks have been reported in the country in the last decade . The aim of this study is to analyze the eco-epidemiological aspects of rickettsial seropositivity...
Rocky Mountain spotted fever is one of the main diseases transmitted by tick bites in Colombia . Studies examining rickettsial seropositivity in humans , potential vectors and amplifying hosts in regions where previous outbreaks occurred are necessary to highlight this disease in the differential diagnosis of febrile s...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "animal", "types", "invertebrates", "medicine", "and", "health", "sciences", "ixodes", "pathology", "and", "laboratory", "medicine", "rocky", "mountain", "spotted", "fever", "pathogens", "domestic", "animals", "geographical", "locations", "microbiology", "vertebrates", ...
2017
Eco-epidemiological analysis of rickettsial seropositivity in rural areas of Colombia: A multilevel approach
Trypanosomiasis induces a remarkable myenteric neuronal degeneration leading to megacolon . Very little is known about the risk for colon cancer in chagasic megacolon patients . To clarify whether chagasic megacolon impacts on colon carcinogenesis , we investigated the risk for colon cancer in Trypanosoma cruzi ( T . c...
The myenteric neuronal activity on colon carcinogenesis is a matter of debate . Chagas disease ( a trypanosomiasis-related chronic infection ) induces megacolon damaging myenteric neurons . Puzzling , tumors have been rarely reported in chagasic megacolon patients . We reveal here hyperplasia-related high-proliferation...
[ "Abstract", "Introduction", "Results", "and", "Discussion" ]
[]
2015
Trypanosomiasis-Induced Megacolon Illustrates How Myenteric Neurons Modulate the Risk for Colon Cancer in Rats and Humans
The epigenetic modification of chromatin structure and its effect on complex neuronal processes like learning and memory is an emerging field in neuroscience . However , little is known about the “writers” of the neuronal epigenome and how they lay down the basis for proper cognition . Here , we have dissected the neur...
Epigenetic regulators can affect gene transcription through modification of DNA and histones , which together form chromatin . The importance of such regulators for cognition is increasingly appreciated , but only few key factors have been identified so far . Excellent candidates are histone modifiers that are involved...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/behavioral", "neuroscience", "molecular", "biology/histone", "modification", "neuroscience/cognitive", "neuroscience", "genetics", "and", "genomics/disease", "models", "developmental", "biology/neurodevelopment", "genetics", "and", "genomics/epigenetics", "neuroscience/...
2011
Epigenetic Regulation of Learning and Memory by Drosophila EHMT/G9a
The rapid advancement of technology in genomics and targeted genetic manipulation has made comparative biology an increasingly prominent strategy to model human disease processes . Predicting orthology relationships between species is a vital component of comparative biology . Dozens of strategies for predicting orthol...
Identifying functionally equivalent proteins between species is a fundamental problem in comparative genetics . While orthology does not guarantee functional equivalence , the identification of orthologs—genes in different organisms that diverged by speciation—is often the first step in approaching this problem . Many ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "machine", "learning", "algorithms", "applied", "mathematics", "vertebrates", "neuroscience", "animals", "simulation", "and", "modeling", "algorithms", "mathematics", "artificial", "intelligence", "phylogenetic", "analysis", "genome", "analysis", "molecular",...
2016
WORMHOLE: Novel Least Diverged Ortholog Prediction through Machine Learning
Management of vector population is a commonly used method for mitigating transmission of mosquito-borne infections , but quantitative information on its practical public health impact is scarce . We study the effectiveness of Ultra-Low Volume ( ULV ) insecticide spraying in public spaces for preventing secondary dengue...
Dengue is a mosquito-borne infection that causes millions of symptomatic infections and thousands of deaths per year . This pathogen is expanding its geographic range to areas that were previously free from autochthonous transmission thanks to the intensification of international travels , urbanization and to climatic ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "dengue", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "radii", "microbiology", "spatial", "epidemiology", "geographical", "locations", "geometry", "animals", "viruses", "mathematics", "rna",...
2019
Effectiveness of Ultra-Low Volume insecticide spraying to prevent dengue in a non-endemic metropolitan area of Brazil
Histone modifications affect DNA–templated processes ranging from transcription to genomic replication . In this study , we examine the cell cycle dynamics of the trimethylated form of histone H3 lysine 4 ( H3K4me3 ) , a mark of active chromatin that is viewed as “long-lived” and that is involved in memory during cell ...
Organisms can inherit information beyond DNA sequence , a phenomenon known as epigenetic inheritance . It is widely believed that chromatin marks provide a carrier for epigenetic information , a hypothesis that is less-supported than generally believed . In this study , we measure the erasure of a “memory” mark of acti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "genetics", "and", "genomics/epigenetics", "genetics", "and", "genomics", "genetics", "and", "genomics/gene", "expression" ]
2010
Replication and Active Demethylation Represent Partially Overlapping Mechanisms for Erasure of H3K4me3 in Budding Yeast
Recent structural and biochemical studies have identified a novel control mechanism of gene expression mediated through the secondary channel of RNA Polymerase ( RNAP ) during transcription initiation . Specifically , the small nucleotide ppGpp , along with DksA , a RNAP secondary channel interacting factor , modifies ...
Control of gene expression is central for cell operation . Transcription regulation is a first step to control gene expression and is largely mediated by DNA-binding factors . These recruit or prevent RNA polymerase binding to promoters of specific genes . Recently , a novel way to control transcription has emerged fro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/microbial", "evolution", "and", "genomics", "genetics", "and", "genomics/gene", "discovery", "molecular", "biology/transcription", "initiation", "and", "activation", "genetics", "and", "genomics/gene", "expression", "microbiology/microbial", "evol...
2009
TraR, a Homolog of a RNAP Secondary Channel Interactor, Modulates Transcription
Mycetoma is a neglected , chronic , and deforming infectious disease caused by fungi and actinomycetes . In Mexico , N . brasiliensis is the predominant etiologic agent . Therapeutic alternatives are necessary because the current drug regimens have several disadvantages . Benzothiazinones ( BTZ ) are a new class of can...
Mycetoma is a neglected tropical disease caused by many etiological agents , including actinobacteria and true fungi . In Mexico , Nocardia brasiliensis and Actinomadura madurae account for more than 90% of the total cases . This subcutaneous infectious disease can affect skin and subcutaneous tissue; actinomycetomas a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
In Vivo Activity of the Benzothiazinones PBTZ169 and BTZ043 against Nocardia brasiliensis
Dengue fever epidemic dynamics are driven by complex interactions between hosts , vectors and viruses . Associations between climate and dengue have been studied around the world , but the results have shown that the impact of the climate can vary widely from one study site to another . In French Guiana , climate-based...
Climatic determinants are amongst the most frequently cited in studies aimed at understanding and explaining the dynamics of vector-borne infections , and dengue in particular . French Guiana , a French overseas territory in which the vector Aedes aegypti is well established , experiences an epidemic cycle of dengue wi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "atmospheric", "science", "tropical", "diseases", "oceans", "epidemiological", "methods", "and", "statistics", "seasons", "ocean", "temperature", "bodies", "of", "water", "neglected", "tropical", "diseases", "infectious", "diseas...
2016
Predicting Dengue Fever Outbreaks in French Guiana Using Climate Indicators
Kaposi's sarcoma-associated herpesvirus ( KSHV ) establishes a latent infection in the host following an acute infection . Reactivation from latency contributes to the development of KSHV-induced malignancies , which include Kaposi's sarcoma ( KS ) , the most common cancer in untreated AIDS patients , primary effusion ...
Kaposi's sarcoma-associated herpesvirus ( KSHV ) is the etiologic agent of all clinical forms of Kaposi's sarcoma ( KS ) and several other malignancies . The life cycle of KSHV consists of latent and lytic phases . While establishment of viral latency is essential for KSHV to evade host immune surveillances , viral lyt...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "medicine", "infectious", "diseases", "basic", "cancer", "research", "biology", "microbiology", "molecular", "cell", "biology", "viral", "diseases" ]
2011
Reactive Oxygen Species Hydrogen Peroxide Mediates Kaposi's Sarcoma-Associated Herpesvirus Reactivation from Latency
Genome-scale metabolic reconstructions are typically validated by comparing in silico growth predictions across different mutants utilizing different carbon sources with in vivo growth data . This comparison results in two types of model-prediction inconsistencies; either the model predicts growth when no growth is obs...
Over the past decade , mathematical models of cellular metabolism have been constructed for describing existing metabolic processes . The gold standard for testing the accuracy and completeness of these models is to compare their cellular growth predictions ( i . e . , cell life/death ) across different scenarios with ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/bioinformatics", "computational", "biology/metabolic", "networks", "computational", "biology/systems", "biology" ]
2009
GrowMatch: An Automated Method for Reconciling In Silico/In Vivo Growth Predictions
Salmonella enterica serovar Typhimurium ( Salmonella ) is one of the most significant food-borne pathogens affecting both humans and agriculture . We have determined that Salmonella encodes an uptake and utilization pathway specific for a novel nutrient , fructose-asparagine ( F-Asn ) , which is essential for Salmonell...
It has long been thought that the nutrient utilization systems of Salmonella would not make effective drug targets because there are simply too many nutrients available to Salmonella in the intestine . Surprisingly , we have discovered that Salmonella relies heavily on a single nutrient during growth in the inflamed in...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "species", "interactions", "biology", "and", "life", "sciences", "microbiology" ]
2014
Fructose-Asparagine Is a Primary Nutrient during Growth of Salmonella in the Inflamed Intestine
Much of the HSV-1 life cycle is carried out in the cell nucleus , including the expression , replication , repair , and packaging of viral genomes . Viral proteins , as well as cellular factors , play essential roles in these processes . Isolation of proteins on nascent DNA ( iPOND ) was developed to label and purify c...
HSV-1 is a human pathogen that infects over 50% of the population . The virus persists as a latent infection in the ganglia of an infected host and upon stressful conditions is reactivated to a lytic state in which it causes recurrent sores at the initial site of infection . During lytic infection , HSV highjacks the h...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Selective Recruitment of Nuclear Factors to Productively Replicating Herpes Simplex Virus Genomes
Chikungunya virus ( CHIKV ) is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness . We sought to characterize the proportion of subclinical and symptomatic CHIKV infections in a prospective cohort study in a country with known CHIK...
Chikungunya virus ( CHIKV ) is a re-emerging mosquito-borne pathogen for which the majority of infections have been considered to result in febrile illness . We sought to characterize the proportion of subclinical and symptomatic CHIKV infections in a prospective cohort of subjects ≥6 months old who underwent active su...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
High Rate of Subclinical Chikungunya Virus Infection and Association of Neutralizing Antibody with Protection in a Prospective Cohort in The Philippines
Mutator strains are expected to evolve when the availability and effect of beneficial mutations are high enough to counteract the disadvantage from deleterious mutations that will inevitably accumulate . As the population becomes more adapted to its environment , both availability and effect of beneficial mutations nec...
Many organisms display increased mutation or recombination rates when exposed to a stressful environment , which can increase the probability that the population acquires adaptations that allow it to avoid extinction . Because of this , it has been suggested that the increase in production rate of genetic variation is ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "antimicrobials", "deletion", "mutation", "medicine", "and", "health", "sciences", "drugs", "variant", "genotypes", "microbiology", "genetic", "mapping", "mutation", "antibiotic", "resistance", "antibiotics", "pharmacology", "antimicrobial", "resistance", "evolutionary", "g...
2017
Stress-induced mutagenesis: Stress diversity facilitates the persistence of mutator genes
The World Health Organization has called for an effort to eliminate Lymphatic Filariasis ( LF ) around the world . In regions where the disease is endemic , local production and distribution of medicated salt dosed with diethylcarbamazine ( DEC ) has been an effective method for eradicating LF . A partner of the Notre ...
As researchers develop more sophisticated technologies , parts of the world are left behind . The front lines of fighting many diseases lie in regions where expensive technology is not feasible . As part of the effort to eradicate lymphatic filariasis in Haiti , our group's goal was to design an assay that would allow ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results/Discussion" ]
[ "medicine", "infectious", "diseases", "analytical", "chemistry", "filariasis", "global", "health", "neglected", "tropical", "diseases", "chemistry", "chemical", "analysis", "parasitic", "diseases" ]
2011
A Low-Tech Analytical Method for Diethylcarbamazine Citrate in Medicated Salt
Bacteria of many species rely on a simple molecule , the intracellular secondary messenger c-di-GMP ( Bis- ( 3'-5' ) -cyclic dimeric guanosine monophosphate ) , to make a vital choice: whether to stay in one place and form a biofilm , or to leave it in search of better conditions . The c-di-GMP network has a bow-tie sh...
How does evolution shape living organisms that seem so well adapted that they could be intelligently designed ? Here , we address this question by analyzing a simple biochemical network that directs social behavior in bacteria; we find that it works analogously to a machine learning algorithm that learns from data . In...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "bacteriology", "biofilms", "taxonomy", "organismal", "evolution", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "pseudomonas", "aeruginosa", "mutation", "phylogenetics", "data", "management", "microbial"...
2017
Bow-tie signaling in c-di-GMP: Machine learning in a simple biochemical network
Vertebrate body designs rely on hydroxyapatite as the principal mineral component of relatively light-weight , articulated endoskeletons and sophisticated tooth-bearing jaws , facilitating rapid movement and efficient predation . Biological mineralization and skeletal growth are frequently accomplished through proteins...
The microstructure of vertebrate bones and teeth is controlled by polyproline-rich protein matrices ( such as amelogenin ) that serve as a scaffold to control the assembly of biological apatites . In tooth enamel , amphibians have large amelogenin subunits and thin enamel while mammals have smaller amelogenin subunits ...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "biochemistry/molecular", "evolution", "developmental", "biology/developmental", "evolution", "developmental", "biology/pattern", "formation", "biochemistry/macromolecular", "assemblies", "and", "machines" ]
2009
Elongated Polyproline Motifs Facilitate Enamel Evolution through Matrix Subunit Compaction
Histone variants , including histone H2A . Z , are incorporated into specific genomic sites and participate in transcription regulation . The role of H2A . Z at these sites remains poorly characterized . Our study investigates changes in the chromatin environment at the Cyclin D1 gene ( CCND1 ) during transcriptional i...
Our study investigates changes in the chromatin environment at the Cyclin D1 gene that are a prerequisite for transcriptional initiation in response to estradiol . Gene expression is under control of chromatin structure . Histone variants , including histone H2A . Z , are incorporated into specific genomic sites and pa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "medicine", "chromosome", "biology", "cell", "growth", "gene", "expression", "genetics", "epigenetics", "biology", "basic", "cancer", "research", "molecular", "cell", "biology", "gene", "function" ]
2013
TIP48/Reptin and H2A.Z Requirement for Initiating Chromatin Remodeling in Estrogen-Activated Transcription
Turnover of regulatory sequence and function is an important part of molecular evolution . But what are the modes of sequence evolution leading to rapid formation and loss of regulatory sites ? Here we show that a large fraction of neighboring transcription factor binding sites in the fly genome have formed from a comm...
Since Jacob and Monod stressed the importance of gene regulation in evolution , our understanding of the mechanisms of regulation has substantially advanced . In higher eukaryotes , genes often have complex regulatory input , which is encoded in cis-regulatory sequence with multiple transcription factor binding sites ....
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "statistical", "mechanics", "genome", "evolution", "population", "genetics", "sequence", "analysis", "genome", "complexity", "evolutionary", "modeling", "biophysics", "theory", "biology", "biophysics", "evolutionary", "genetics", "physics", "genomics", "evolutionary", "biol...
2011
Formation of Regulatory Modules by Local Sequence Duplication
G-quadruplex or G4 DNA is a non-B secondary DNA structure that comprises a stacked array of guanine-quartets . Cellular processes such as transcription and replication can be hindered by unresolved DNA secondary structures potentially endangering genome maintenance . As G4-forming sequences are highly frequent througho...
Genome instability is not evenly distributed , but rather is highly elevated at certain genomic loci containing DNA sequences that can fold into non-canonical secondary structures . The four-stranded G-quadruplex or G4 DNA is one such DNA structure capable of instigating transcription and/or replication obstruction and...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "forms", "of", "dna", "genetics", "biology", "and", "life", "sciences", "dna", "structure", "dna", "dna", "recombination", "molecular", "genetics" ]
2014
Topoisomerase I Plays a Critical Role in Suppressing Genome Instability at a Highly Transcribed G-Quadruplex-Forming Sequence
Sequencing of the T cell receptor ( TCR ) repertoire is a powerful tool for deeper study of immune response , but the unique structure of this type of data makes its meaningful quantification challenging . We introduce a new method , the Gamma-GPD spliced threshold model , to address this difficulty . This biologically...
A more detailed understanding of the immune response can unlock critical information concerning diagnosis and treatment of disease . Here , in particular , we study T cells through T cell receptor sequencing , as T cells play a vital role in immune response . One important feature of T cell receptor sequencing data is ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "inflammatory", "diseases", "rheumatology", "medicine", "and", "health", "sciences", "cluster", "analysis", "immune", "cells", "hierarchical", "clustering", "immunology", "blastomas", "cancers", "and", "neoplasms", "cloning", "oncology", "sarcoidosis", ...
2018
powerTCR: A model-based approach to comparative analysis of the clone size distribution of the T cell receptor repertoire
Experimental studies have revealed evidence of both parts-based and holistic representations of objects and faces in the primate visual system . However , it is still a mystery how such seemingly contradictory types of processing can coexist within a single system . Here , we propose a novel theory called mixture of sp...
Does the brain represent an object as a combination of parts or as a whole ? Past experiments have found both types of representation; but how can such opposing notions coexist in a single visual system ? Here , we introduce a novel theory called mixture of sparse coding models for investigating the possible computatio...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "face", "social", "sciences", "vertebrates", "neuroscience", "learning", "and", "memory", "animals", "mammals", "simulation", "and", "modeling", "face", "recognition", "perception", "primates", "cognitive", "psychology", "cogniti...
2017
A mixture of sparse coding models explaining properties of face neurons related to holistic and parts-based processing
Nervous system function requires proper development of two functional and morphological domains of neurons , axons and dendrites . Although both these domains are equally important for signal transmission , our understanding of dendrite development remains relatively poor . Here , we show that in C . elegans the Wnt li...
Neurons have distinct compartments , which include axons and dendrites . Both of these compartments are essential for communication between neurons , as signals are received by dendrites and transmitted by axons . Although dendrites are vital for neural connectivity , very little is known about how they are formed . He...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "models", "morphogens", "developmental", "biology", "caenorhabditis", "elegans", "cellular", "neuroscience", "model", "organisms", "developmental", "neuroscience", "neuronal", "morphology", "axon", "guidance", "molecular", "development", "biology", "neuroscience", ...
2011
LIN-44/Wnt Directs Dendrite Outgrowth through LIN-17/Frizzled in C. elegans Neurons
With the advent of whole exome sequencing , cases where no pathogenic coding mutations can be found are increasingly being observed in many diseases . In two large , distantly-related families that mapped to the Charcot-Marie-Tooth neuropathy CMTX3 locus at chromosome Xq26 . 3-q27 . 3 , all coding mutations were exclud...
Next generation sequencing technologies have greatly advanced disease gene discovery for Charcot-Marie-Tooth ( CMT ) disease and related inherited peripheral neuropathies . However , many families with CMT remain unsolved after all protein-coding sequences have been interrogated through whole exome sequencing . The pat...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "charcot-marie-tooth", "disease", "insertion", "mutation", "genetic", "diseases", "mutation", "chromosome", "mapping", "molecular", "biology", "techniques", "research", "and", "analysis", "methods", "neuropathy", "sex", "chromosome...
2016
Whole Genome Sequencing Identifies a 78 kb Insertion from Chromosome 8 as the Cause of Charcot-Marie-Tooth Neuropathy CMTX3
Visceral leishmaniasis is a potentially fatal infectious disease caused by the protozoan parasite Leishmania infantum/chagasi in the New World , or by L . donovani or L . infantum/chagasi in the Old World . Infection leads to a variety of outcomes ranging from asymptomatic infection to active disease , characterized by...
Visceral leishmaniasis ( VL ) is a potentially fatal vector-borne infectious disease that leads to a variety of outcomes ranging from asymptomatic infection to symptomatic disease . In northeast Brazil , the etiological agent of VL is the protozoan Leishmania chagasi/infantum . Active VL is characterized by fevers , we...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/gene", "expression", "infectious", "diseases/neglected", "tropical", "diseases", "immunology/immune", "response", "immunology/innate", "immunity", "infectious", "diseases/protozoal", "infections", "immunology/leukocyte", "signaling", "and", "gene", ...
2008
Macrophage and T-Cell Gene Expression in a Model of Early Infection with the Protozoan Leishmania chagasi
Developing intervention strategies for the control of parasitic nematodes continues to be a significant challenge . Genomic and post-genomic approaches play an increasingly important role for providing fundamental molecular information about these parasites , thus enhancing basic as well as translational research . Her...
Lymphatic filariasis , also known as elephantiasis , is a tropical disease affecting over 120 million people worldwide . More than 40 million people live with painful , disfiguring symptoms that can cause severe debilitation and social stigma . The disease is caused by infection with thread-like filarial nematodes ( ro...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "global", "health", "biology", "genomics", "genetics", "and", "genomics" ]
2011
A Deep Sequencing Approach to Comparatively Analyze the Transcriptome of Lifecycle Stages of the Filarial Worm, Brugia malayi
Centromeres typically contain tandem repeat sequences , but centromere function does not necessarily depend on these sequences . We identified functional centromeres with significant quantitative changes in the centromeric retrotransposons of wheat ( CRW ) contents in wheat aneuploids ( Triticum aestivum ) and the offs...
Chromosomal rearrangements during the formation of wheat aneuploids and their wide hybrids caused reduction , elimination or expansion of the centromeric retrotransposon sequences and the formation of multiple centromeres . Centromere function was not affected by centromeric sequence elimination , which was revealed by...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biotechnology", "nuclear", "staining", "retrotransposons", "chromosome", "structure", "and", "function", "centromeres", "dicentric", "chromosomes", "cereal", "crops", "plant", "science", "genetic", "elements", "crops", "plant", "genomics", "plants", "research", "and", ...
2016
De Novo Centromere Formation and Centromeric Sequence Expansion in Wheat and its Wide Hybrids
Protozoan parasites of the genus Giardia are highly prevalent globally , and infect a wide range of vertebrate hosts including humans , with proliferation and pathology restricted to the small intestine . This narrow ecological specialization entailed extensive structural and functional adaptations during host-parasite...
Organelles with endosymbiotic origin are present in virtually all extant eukaryotes and have undergone considerable remodeling during > 1 billion years of evolution . Highly diverged organelles such as mitosomes or plastids in some parasitic protozoa are the product of extensive secondary reduction . They are sufficien...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusion" ]
[ "protein", "interactions", "giardia", "protein", "interaction", "networks", "parasitic", "protozoans", "membrane", "proteins", "protozoans", "outer", "membrane", "proteins", "network", "analysis", "mitochondria", "bioenergetics", "cellular", "structures", "and", "organelles...
2016
An Interactome-Centered Protein Discovery Approach Reveals Novel Components Involved in Mitosome Function and Homeostasis in Giardia lamblia
Protein domains are basic functional units of proteins . Many protein domains are pervasive among diverse biological processes , yet some are associated with specific pathways . Human complex diseases are generally viewed as pathway-level disorders . Therefore , we hypothesized that pathway-specific domains could be hi...
Protein domains are basic functional units of proteins , yet domain-based pathway annotations for proteins are challenging tasks because many domains are pervasive among diverse pathways . Therefore , we developed a network-based scoring scheme to measure pathway specificity of domains , and then used it to identify pa...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "fish", "protein", "interactions", "protein", "interaction", "networks", "vertebrates", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "model", "organisms", "coro...
2019
Pathway-specific protein domains are predictive for human diseases
Initial studies of 88 transmission pairs in the Zambia Emory HIV Research Project cohort demonstrated that the number of transmitted HLA-B associated polymorphisms in Gag , but not Nef , was negatively correlated to set point viral load ( VL ) in the newly infected partners . These results suggested that accumulation o...
In the majority of HIV-1 cases , a single virus establishes infection . However , mutations in the viral genome accumulate over time in order to avoid recognition by the host immune response . Certain mutations in the main structural protein , Gag , driven by cytotoxic T lymphocytes are detrimental to viral replication...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "immunodeficiency", "viruses", "major", "histocompatibility", "complex", "viral", "immune", "evasion", "viral", "transmission", "and", "infection", "virology", "immunology", "biology", "microbiology", "viral", "replication", "viral", "load" ]
2012
Role of Transmitted Gag CTL Polymorphisms in Defining Replicative Capacity and Early HIV-1 Pathogenesis
TGFβs act through canonical and non-canonical pathways , and canonical signals are transduced via Smad2 and Smad3 . However , the contribution of canonical vs . non-canonical pathways in cartilage is unknown because the role of Smad2 in chondrogenesis has not been investigated in vivo . Therefore , we analyzed mice in ...
The cartilage growth plate regulates the size and shape of nearly every skeletal element in the body . TGFβs are potent inducers of cartilage formation , but the mechanisms by which they transduce their signals in cartilage during development are poorly understood . Similarly , there is strong evidence that dysregulati...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "growth", "plate", "medicine", "and", "health", "sciences", "chondrocytes", "gene", "regulation", "dna-binding", "proteins", "bone", "connective", "tissue", "cells", "cartilage", "small", "interfering", "rnas", "animal", "cells", "proteins", "gene", "expression", "con...
2016
Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate
Sequence-based protein homology detection has been extensively studied and so far the most sensitive method is based upon comparison of protein sequence profiles , which are derived from multiple sequence alignment ( MSA ) of sequence homologs in a protein family . A sequence profile is usually represented as a positio...
Sequence-based protein homology detection has been extensively studied , but it remains very challenging for remote homologs with divergent sequences . So far the most sensitive methods employ HMM-HMM comparison , which models a protein family using HMM ( Hidden Markov Model ) and then detects homologs using HMM-HMM al...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biology", "computational", "biology" ]
2014
MRFalign: Protein Homology Detection through Alignment of Markov Random Fields
Chronic activation of the immune system in HIV infection is one of the strongest predictors of morbidity and mortality . As such , approaches that reduce immune activation have received considerable interest . Previously , we demonstrated that administration of a type I interferon receptor antagonist ( IFN-1ant ) durin...
Innate and adaptive immune activation is one of the strongest predictors of HIV disease progression to AIDS and non-AIDS morbidity and mortality . Type I interferon ( IFN-I ) signaling is a major driver of such activation even in ART-treated people . Therefore , manipulation of IFN-I signaling has been proposed as a th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "t", "helper", "cells", "medicine", "and", "health", "sciences", "body", "fluids", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "microbiology", "vertebrates", "animals", "mammals", "retroviruses", "virus...
2018
Type I IFN signaling blockade by a PASylated antagonist during chronic SIV infection suppresses specific inflammatory pathways but does not alter T cell activation or virus replication
P-glycoprotein ( Pgp ) and multidrug resistance-associated proteins ( MRPs ) are ATP-dependent transporters involved in efflux of toxins and xenobiotics from cells . When overexpressed , these transporters can mediate multidrug resistance ( MDR ) in mammalian cells , and changes in Pgp expression and sequence are assoc...
Schistosomes are parasitic flatworms that are the causative agents of schistosomiasis , a major tropical disease . As adults , schistosomes reside within the host vasculature , taking up nutrients , evading host defenses , and expelling wastes and toxins . Multidrug resistance transporters are involved in removal of to...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "schistosomiasis", "drug", "research", "and", "development", "drugs", "and", "devices", "neglected", "tropical", "diseases", "pharmacology", "parasitic", "diseases", "drug", "discovery" ]
2011
Genetic Knockdown and Pharmacological Inhibition of Parasite Multidrug Resistance Transporters Disrupts Egg Production in Schistosoma mansoni
Caloric/dietary restriction ( CR/DR ) can promote longevity and protect against age-associated disease across species . The molecular mechanisms coordinating food intake with health-promoting metabolism are thus of significant medical interest . We report that conserved Caenorhabditis elegans microRNA-80 ( mir-80 ) is ...
Dietary restriction , limitation of calorie intake with maintained vitamin and mineral support , can extend lifespan and protect against diseases of age across many species . Elaboration of molecular mechanisms that control dietary restriction in simple animal models may therefore inform on strategies to activate healt...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Deletion of microRNA-80 Activates Dietary Restriction to Extend C. elegans Healthspan and Lifespan
In a pioneering cross-sectional study among Bolivian immigrants in the city of São Paulo , Brazil , the epidemiological profile , clinical manifestations and morbidity of Chagas disease were described . The feasibility of the management of Chagas disease at primary healthcare clinics using a biomedical and psychosocial...
Chagas disease affects approximately 6 million Latin American people . It is considered a neglected tropical disease since it mainly affects vulnerable , poverty-stricken people . Public health policies and investments in research on new treatment and control instruments have not been prioritized . In fact , disease ur...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "physicians", "medical", "doctors", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "maternal", "health", "obstetrics", "and", "gynecology", "immunology", "tropical", "diseases", "parasitic", "disease...
2017
Interdisciplinary approach at the primary healthcare level for Bolivian immigrants with Chagas disease in the city of São Paulo
Plasmodium salivary sporozoites are the infectious form of the malaria parasite and are dormant inside salivary glands of Anopheles mosquitoes . During dormancy , protein translation is inhibited by the kinase UIS1 that phosphorylates serine 59 in the eukaryotic initiation factor 2α ( eIF2α ) . De-phosphorylation of eI...
Malaria is transmitted to humans by female mosquitoes as they take a blood meal . Plasmodium sporozoites are the infectious and quiescent forms of malaria parasites , which reside in the salivary glands of mosquitoes . Global protein synthesis is inhibited in sporozoites through phosphorylation of the translational fac...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
UIS2: A Unique Phosphatase Required for the Development of Plasmodium Liver Stages
Dengue has emerged as the most important vector-borne viral disease in tropical areas . Evaluations of the burden and severity of dengue disease have been hindered by the frequent lack of laboratory confirmation and strong selection bias toward more severe cases . A multinational , prospective clinical study was carrie...
Dengue is the most important mosquito-borne viral disease in humans . This disease is now endemic in more than 100 countries and threatens more than 2 . 5 billion people living in tropical countries . It currently affects about 50 to 100 million people each year . It causes a wide range of symptoms , from an inapparent...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "clinical", "research", "design", "epidemiology", "biology", "microbiology" ]
2012
Clinical and Virological Study of Dengue Cases and the Members of Their Households: The Multinational DENFRAME Project
Homologous recombination ( HR ) is critical for the repair of double strand breaks and broken replication forks . Although HR is mostly error free , inherent or environmental conditions that either suppress or induce HR cause genomic instability . Despite its importance in carcinogenesis , due to limitations in our abi...
Cancer is a disease of the genome , caused by accumulated genetic changes , such as point mutations and large-scale sequence rearrangements . Homologous recombination ( HR ) is a critical DNA repair pathway . While generally accurate , HR between misaligned sequences or between homologous chromosomes can lead to insert...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "mutagenesis", "biochemistry", "mutation", "medicine", "and", "health", "sciences", "cell", "biology", "nucleic", "acids", "genetics", "cancer", "treatment", "biology", "and", "life", "sciences", "dna", "dna", "repair", "dna", "recombination", "molecular",...
2014
Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals In Vivo
The Leishmania OligoC-TesT and NASBA-Oligochromatography ( OC ) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens . We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis ( VL ) , cutaneous leishmaniasis ( CL ...
The leishmaniases are a group of vector-borne diseases caused by protozoan parasites of the genus Leishmania . The parasites are transmitted by phlebotomine sand flies and can cause , depending on the infecting species , three clinical manifestations of leishmaniasis: visceral leishmaniasis ( VL ) , post kala-azar derm...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/protozoal", "infections" ]
2010
Diagnostic Accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for Diagnosis of Leishmaniasis in Sudan
Tuberculosis ( TB ) is an escalating global health problem and improved vaccines against TB are urgently needed . HLA-E restricted responses may be of interest for vaccine development since HLA-E displays very limited polymorphism ( only 2 coding variants exist ) , and is not down-regulated by HIV-infection . The pepti...
Mycobacterium tuberculosis ( Mtb ) has infected about one-third of the world population , resulting in fatal pulmonary tuberculosis ( TB ) in 1 . 5 million people annually . Vaccination against Mtb has decreased disease incidence in young children but does not prevent pulmonary TB in adults . The immune response agains...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "immunology/immunity", "to", "infections", "immunology/immune", "response", "microbiology/immunity", "to", "infections" ]
2010
Mycobacterium tuberculosis Peptides Presented by HLA-E Molecules Are Targets for Human CD8+ T-Cells with Cytotoxic as well as Regulatory Activity
Chromosome organizations of related bacterial genera are well conserved despite a very long divergence period . We have assessed the forces limiting bacterial genome plasticity in Escherichia coli by measuring the respective effect of altering different parameters , including DNA replication , compositional skew of rep...
Genomic analyses have revealed that bacterial genomes are dynamic entities that evolve through various processes including intrachromosome genetic rearrangements , gene duplication , and gene loss or acquisition by gene transfer . Nevertheless , comparison of bacterial chromosomes from related genera revealed a conserv...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology", "genetics", "and", "genomics", "microbiology", "eubacteria" ]
2007
Chromosome Structuring Limits Genome Plasticity in Escherichia coli
Mutations in Nesprin-1 and 2 ( also called Syne-1 and 2 ) are associated with numerous diseases including autism , cerebellar ataxia , cancer , and Emery-Dreifuss muscular dystrophy . Nesprin-1 and 2 have conserved orthologs in flies and worms called MSP-300 and abnormal nuclear Anchorage 1 ( ANC-1 ) , respectively . T...
The molecular mechanisms that underpin synapse formation and axon termination are central to forming a functional , fully connected nervous system . The PHR proteins are important regulators of neuronal development that function in axon outgrowth and termination , as well as synapse formation . Here we describe the dis...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience", "genetics", "biochemistry", "biology", "and", "life", "sciences" ]
2014
The Nesprin Family Member ANC-1 Regulates Synapse Formation and Axon Termination by Functioning in a Pathway with RPM-1 and β-Catenin
During infection , pathogens must utilise the available nutrient sources in order to grow while simultaneously evading or tolerating the host’s defence systems . Amino acids are an important nutritional source for pathogenic fungi and can be assimilated from host proteins to provide both carbon and nitrogen . The hpdA ...
Fungi that infect humans are a major health problem , especially for those with compromised immune systems . Many fungal infections are extremely difficult to cure and if left untreated are fatal . For successful infection to occur , the fungal pathogen must be able to grow by acquiring and utilising the available nutr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Intracellular Growth Is Dependent on Tyrosine Catabolism in the Dimorphic Fungal Pathogen Penicillium marneffei
Cornelia de Lange Syndrome ( CdLS ) is the founding member of a class of multi-organ system birth defect syndromes termed cohesinopathies , named for the chromatin-associated protein complex cohesin , which mediates sister chromatid cohesion . Most cases of CdLS are caused by haploinsufficiency for Nipped-B-like ( Nipb...
Although best known for its role in chromatid cohesion , cohesin is increasingly seen as a regulator of gene expression . In Cornelia de Lange Syndrome ( CdLS ) , partial deficiency for NIPBL , which encodes a cohesin regulator , is associated with small changes in the expression of many genes ( similar effects are see...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "teratology", "gene", "regulation", "cell", "differentiation", "animal", "models", "developmental", "biology", "model", "organisms", "organism", "development", "heart", "development", "molecular", "genetics", "morphogenesis", "chromatin", "embryology", "birth", ...
2011
Multifactorial Origins of Heart and Gut Defects in nipbl-Deficient Zebrafish, a Model of Cornelia de Lange Syndrome
Malaria in pregnancy is exquisitely aggressive , causing a range of adverse maternal and fetal outcomes prominently linked to Plasmodium-infected erythrocyte cytoadherence to fetal trophoblast . To elucidate the physiopathology of infected erythrocytes ( IE ) sequestration in the placenta we devised an experimental sys...
Malaria in pregnancy is exquisitely aggressive , causing a range of adverse effects impacting maternal and fetal health . Many of those effects are thought to derive from placental sequestration of red blood cells infected with the malaria parasite ( Plasmodium falciparum ) eliciting a placental inflammatory response t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "malaria", "parasitic", "diseases" ]
2013
Intravital Placenta Imaging Reveals Microcirculatory Dynamics Impact on Sequestration and Phagocytosis of Plasmodium-Infected Erythrocytes