IdA stringlengths 6 21 | IdB stringlengths 6 21 | labels int64 0 2 | mechanism stringclasses 40
values | effect stringclasses 10
values | score float64 0.1 0.99 ⌀ | sentence stringlengths 10 1.63k ⌀ | signor_id stringlengths 12 14 |
|---|---|---|---|---|---|---|---|
Q99835 | Q13635 | 2 | binding | down-regulates activity | 0.786 | We show that free Ptc (unbound by Hh) acts sub-stoichiometrically to suppress Smo activity and thus is critical in specifying the level of pathway activity. | SIGNOR-91709 |
P38936 | P04637 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.876 | The ability of p53 to activate transcription from specific sequences suggests that genes induced by p53 may mediate its biological role as a tumor suppressor. Using a subtractive hybridization approach, we identified a gene, named WAF1, whose induction was associated with wild-type but not mutant p53 gene expression in... | SIGNOR-37145 |
Q14185 | Q9Y5X3 | 2 | binding | up-regulates activity | 0.346 | SNX5 Is a Novel Binding Partner of the DHR1 Domain of DOCK180. In summary, we found that DOCK180 regulates transport of CI-MPR via SNX5 binding. | SIGNOR-269441 |
O43521 | P10415 | 2 | binding | down-regulates | 0.819 | Bim can induce apoptosis by interacting with anti-apoptotic members of the bcl2 family, including bcl2, bcl-xl and mcl-1.. Bim binds prosurvival proteins comparably. The members that promote cell survival, including mammalian bcl-2, bcl-xl,bcl-w, mcl-1, and a1 | SIGNOR-133823 |
Q00535 | P12931 | 1 | phosphorylation | up-regulates | 0.39 | These results present compelling evidence that cdk5/p35 kinase is responsible for the novel phosphorylation of c-src at ser75 in neuronal cells, raising the intriguing possibility that c-src acts as an effector of cdk5/p35 kinase during neuronal development. | SIGNOR-71950 |
Q99941 | P11021 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.448 | Accordingly, N-terminal fragments of each ATF6 isoform (N-ATF6α and N-ATF6β) were overexpressed in HeLa cells and the effects on GRP78 induction were assessed. When expressed at similar levels, N-ATF6α conferred ∼200-fold greater GRP78 promoter activation than N-ATF6β. | SIGNOR-261566 |
O95835 | P49593 | 0 | dephosphorylation | down-regulates activity | 0.2 | POPX2 is capable of dephosphorylating LATS1 at Thr1079 (Figure xref ), which is a residue critical for LATS1 activity.|POPX2 might negatively regulate the activities of MST1 and LATS1 through dephosphorylation.|We found that POPX2 could dephosphorylate LATS1 on Threonine-1079, leading to inactivation of LATS1 kinase. | SIGNOR-276989 |
P28482 | Q08493 | 1 | phosphorylation | down-regulates | 0.256 | The short-form pde4b2 isoenzyme was activated by erk2 phosphorylation. sub-family selective actions in the ability of erk2 map kinase to phosphorylate and regulate the activity of pde4 cyclic amp-specific phosphodiesterases | SIGNOR-83187 |
Q02224 | O43663 | 2 | binding | up-regulates activity | 0.584 | These data indicate that PRC1 binds to KIF4, MKLP1 and CENP-E during late mitosis; however, it apparently does not interact simultaneously with more than one of these motor proteins. | SIGNOR-265990 |
P46531 | P49768 | 0 | cleavage | up-regulates | 0.797 | Presenilin-1 (ps1), a polytopic membrane protein primarily localized to the endoplasmic reticulum, is required for efficient proteolysis of both notch and beta-amyloid precursor protein (app) within their trans- membrane domains. | SIGNOR-72886 |
P68400 | Q8NEL9 | 1 | phosphorylation | down-regulates activity | 0.2 | Here we incubated a recombinant preparation of the phospholipase in vitro with several enzymes including protein kinase CK2 (CK2), extracellular signal-regulated kinase 2 (ERK2), and protein phosphatase 2A (PP2A) to identify effects that might be of regulatory importance in vivo.Major findings were that 1) CK2 phosphor... | SIGNOR-262977 |
Q9UKV8 | P49137 | 0 | phosphorylation | up-regulates | 0.436 | Mk2 was found to phopsphorylate in vitro the ago2 protein in ser387, which was identified as the major ago2 phosphorylation site in cells.and mutation of ser387 to alanineimpairsago2 localization to therna-containing granules termedprocessing bodies | SIGNOR-166615 |
P17676 | P28482 | 0 | phosphorylation | up-regulates | 0.716 | Phosphorylation of cebpb at thr(235) peaked at 16 hours in il-1beta-stimulated cells. The mek inhibitor u0126 inhibited this phosphorylation and reduced mmp-1 gene induction. | SIGNOR-187798 |
P29275 | P38405 | 2 | binding | up-regulates activity | 0.524 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256910 |
O96017 | P18887 | 1 | phosphorylation | up-regulates | 0.515 | Chk2 formed a complex with xrcc1, the ber scaffold protein, and phosphorylated xrcc1 in vivo and in vitro at thr(284). our results are consistent with the phosphorylation of xrcc1 by atm-chk2 facilitating recruitment of downstream ber proteins to the initial damage recognition/excision step to promote ber. | SIGNOR-181816 |
P50613 | P11802 | 1 | phosphorylation | up-regulates | 0.587 | Phosphorylation of cdk4 on threonine 172 by a cdk-activating kinase (cak). therefore, formation of the cyclin d-cdk4 complex and phosphorylation of the bound catalytic subunit are independently regulated, and in addition to the requirement for cak activity, serum stimulation is required to promote assembly of the comp... | SIGNOR-36549 |
Q9BZL6 | P17252 | 0 | phosphorylation | up-regulates activity | 0.393 | Our data demonstrate that gastrin-stimulated PKD2 activation involves a heterotrimeric G alpha(q) protein as well as the activation of phospholipase C. Furthermore, we show that PKD2 can be activated by classical and novel members of the protein kinase C (PKC) family such as PKC alpha, PKC epsilon, and PKC eta.|The pos... | SIGNOR-275955 |
O75581 | Q9HCP0 | 0 | phosphorylation | up-regulates | 0.545 | Ck1gamma is associated with lrp6, which has multiple, modular ck1 phosphorylation sites. Wnt treatment induces the rapid ck1gamma-mediated phosphorylation of these sites within lrp6 | SIGNOR-143029 |
Q9UH99 | P20339 | 2 | binding | up-regulates activity | 0.42 | Rab5ip represents a novel rab5 interacting protein that may function on endocytic vesicles as a receptor for rab5-GDP and participate in the activation of rab5 | SIGNOR-261309 |
P11142 | Q9UNE7 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.728 | BAG-1 stimulates CHIP-induced degradation of the glucocorticoid hormone receptor (GR). A model for the cooperation of CHIP and BAG-1 in coupling Hsc/Hsp70 to the ubiquitin/proteasome system. CHIP associates with Hsc/Hsp70 via its TPR chaperone adaptor (TPR) and, at the same time, recruits E2 ubiquitin-conjugating enzym... | SIGNOR-272588 |
P68400 | Q15185 | 1 | phosphorylation | up-regulates | 0.354 | Several lines of evidence suggest that a cpges-activating protein kinase is ck-ii (casein kinase ii). Recombinant cpges was phosphorylated directly by and associated with ck-ii in vitro, resulting in marked reduction of the k m for the substrate pgh2. | SIGNOR-123594 |
Q9NRY4 | Q6PJ69 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.2 | Ubiquitin ligase TRIM65 promotes colorectal cancer metastasis by targeting ARHGAP35 for protein degradation | SIGNOR-272256 |
Q8N6T7 | P31749 | 0 | phosphorylation | down-regulates activity | 0.532 | AKT1 interacts with and phosphorylates SIRT6 on Ser 338.|Because AKT1 suppresses SIRT6 protein abundance by decreasing its stability, we investigated whether MDM2 is involved in this process. | SIGNOR-278465 |
Q96HS1 | Q99683 | 1 | dephosphorylation | up-regulates activity | 0.437 | PGAM5 Is a Protein Ser/Thr Phosphatase That Activates ASK1. PGAM5 Dephosphorylates ASK1. This dephosphorylation unleashes phosphorylation ofThr-838 in the kinase domain, with activation of ASK1. | SIGNOR-277984 |
O60231 | Q92917 | 2 | binding | up-regulates quantity | 0.848 | In this report, we showed that GPKOW interacted directly with the DHX16/hPRP2 and with RNA. Immuno-depletion of GPKOW from HeLa nuclear extracts resulted in an inactive spliceosome that still bound DHX16. | SIGNOR-266312 |
P22087 | Q92793 | 0 | acetylation | down-regulates activity | 0.27 | Here, we show that FBL is acetylated at several lysine residues by the acetyltransferase CBP and deacetylated by SIRT7.|hyperacetylation impairs the interaction of FBL with histone H2A and chromatin, thereby compromising H2AQ104 methylation (H2AQ104me) and rDNA transcription. SIRT7-dependent deacetylation of FBL ensure... | SIGNOR-275897 |
Q9GZQ8 | Q05513 | 0 | phosphorylation | down-regulates activity | 0.2 | LC3B is phosphorylated at Thr-50 within the LDS by serine/threonine kinase (STK) 3 and STK4. Here, we identified LIR motifs in STK3 and atypical protein kinase Cζ (PKCζ) and never in mitosis A (NIMA)-related kinase 9 (NEK9). All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-5... | SIGNOR-273906 |
P08754 | P34972 | 2 | binding | up-regulates activity | 0.452 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256843 |
P50148 | P43657 | 2 | binding | up-regulates | 0.411 | Lysophosphatidic acid (lpa), a major g protein coupled receptor (gpcr)-activating ligand present in serum, elicits growth factor like responses by stimulating specific gpcrs coupled to heterotrimeric g proteins such as g(i), g(q), and g12/13. | SIGNOR-135822 |
P29353 | P07949 | 2 | binding | up-regulates | 0.656 | We have shown that the sh2 domain of the adaptor protein shc coimmunoprecipitates with all the ret. | SIGNOR-36902 |
P60484 | Q05397 | 1 | dephosphorylation | down-regulates activity | 0.821 | The tumor suppressor PTEN is a phosphatase with sequence homology to tensin. PTEN dephosphorylates phosphatidylinositol 3,4, 5-trisphosphate (PIP3) and focal adhesion kinase (FAK), and it can inhibit cell growth, invasion, migration, and focal adhesions. We investigated molecular interactions of PTEN and FAK in gliobla... | SIGNOR-248547 |
O76061 | Q16665 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.297 | With the ChIP assay, we demonstrated the direct binding of HIF-1alpha to STC2 promoter. These findings support the notion that HIF-1 is a potent stimulator of STC2 expression. Collectively, this is the first report to show that STC2 was aberrantly hypermethylated in human cancer cells. The findings demonstrated that ST... | SIGNOR-260389 |
Q38SD2 | P30622 | 1 | phosphorylation | up-regulates activity | 0.398 | LRRK1 phosphorylates CLIP-170 at Thr1384, located in its C-terminal zinc knuckle motif, and this promotes the association of CLIP-170 with dynein-dynactin complexes. | SIGNOR-275469 |
Q9UHD2 | Q14164 | 2 | binding | up-regulates activity | 0.646 | Whereas nemo assembles some but not all ikk complexes [12,13], recent reports provide strong experimental evidence for a role of tank [also called traf-interacting protein (i-traf)], nak-associated protein (nap1) and similar to nap1 tbk1 adaptor (sintbad) in the assembly of tbk1 and ikk-e kinase complexes that phosphor... | SIGNOR-178053 |
P25090 | O95837 | 2 | binding | up-regulates activity | 0.435 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256888 |
P14672 | O00141 | 0 | phosphorylation | up-regulates activity | 0.312 | We evaluated the putative role of sgk1 in the modulation of glut4. Coexpression of the kinase along with glut4 in xenopus oocytes stimulated glucose transport. The enhanced glut4 activity was paralleled by increased transporter abundance in the plasma membrane. Disruption of the sgk1 phosphorylation site on glut4 ((s27... | SIGNOR-236653 |
O14522 | P06241 | 0 | phosphorylation | down-regulates activity | 0.414 | Synapse formation by PTPRT was inhibited by phosphorylation of tyrosine 912 within the membrane-proximal catalytic domain of PTPRT by Fyn. This tyrosine phosphorylation reduced phosphatase activity of PTPRT | SIGNOR-275543 |
Q05655 | P49815 | 1 | phosphorylation | down-regulates activity | 0.2 | In vivo kinase analysis further indicated that both S932 and S939 are phosphorylated in response to translation inhibitors. Finally, phosphorylation defective TSC2 mutants (S932A and S939A single mutants and a S932A/S939A double mutant) failed to upregulate mTORC1 activity in the presence of translation inhibitors, sug... | SIGNOR-277427 |
Q13261 | P14784 | 2 | binding | up-regulates | 0.8 | The il-15 receptor comprises a heterotrimeric complex consisting of the common ?cytokine Receptor (?c), the il-2 ? Receptor subunit (il-2r?), And an il-15-specific ? Receptor (il-15r?) | SIGNOR-157418 |
P53671 | Q15672 | 1 | phosphorylation | up-regulates quantity | 0.2 | LIMK2 directly phosphorylated TWIST1, indicating that LIMK2 also regulates TWIST1 post-translationally (XREF_FIG).|LIMK2 positively regulates TWIST1 protein levels. | SIGNOR-278952 |
P52954 | P37275 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.326 | Compared with the empty vector, LBX1 induced increased promoter activity of threefold to fourfold and fivefold to sixfold for ZEB1 and Snail1, respectively | SIGNOR-266054 |
P63096 | P08913 | 2 | binding | up-regulates activity | 0.556 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256698 |
P31749 | Q16875 | 1 | phosphorylation | up-regulates | 0.427 | We also found that AMP activated protein kinase and protein kinases A, B, and C catalyzed the phosphorylation of Ser-460 of HBP1, and that in addition both isoforms are phosphorylated at a second, as yet undetermined site by protein kinase C. However, none of the phosphorylations had any effect on the intrinsic kinetic... | SIGNOR-252477 |
P42224 | P42226 | 2 | binding | down-regulates activity | 0.488 | STAT6 mediates suppression of STAT1 and NF-kB-dependent transcription by distinct mechanisms. Both processes are dependent upon the STAT6 transactivation domain and may involve sequestration of necessary but different transcriptional coactivator proteins. These two suppressive mechanisms are controlled differentially b... | SIGNOR-249552 |
P68400 | Q9Y4K3 | 2 | binding | up-regulates activity | 0.2 | Here, we show that somatic nuclear autoantigenic sperm protein (sNASP) binds to TRAF6 to prevent TRAF6 autoubiquitination in unstimulated macrophages. Following LPS stimulation, a complex consisting of sNASP, TRAF6, IRAK4, and casein kinase 2 (CK2) is formed. CK2 phosphorylates sNASP at serine 158, allowing sNASP to di... | SIGNOR-273656 |
P62714 | P10415 | 1 | dephosphorylation | up-regulates activity | 0.385 | The phosphorylation of Bcl-2 resulted in a reduction in anti-apoptotic function, implying that dephosphorylation promoted the anti-apoptotic activity of Bcl-2 protein in human tumor cell lines. Thus, the present findings suggest that ERK and PP2A are physiological regulators of Bcl-2 phosphorylation, and these enzymes ... | SIGNOR-248589 |
Q9HAW8 | P20823 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.251 | Using gel shift and functional assays, HNF1alpha was demonstrated to bind to and activate the UGT1A8, -1A9, and -1A10 promoters. In contrast, Cdx2 bound to and activated the UGT1A8 and -1A10 promoters but could not activate the UGT1A9 promoter. | SIGNOR-253971 |
P17612 | O00418 | 1 | phosphorylation | up-regulates activity | 0.305 | EEF-2K can be phosphorylated in vitro by cAMP-dependent protein kinase (PKA) and that this induces significant Ca(2+)/calmodulin (CaM)-independent eEF-2K activity. sites of phosphorylation were Ser-365 and Ser-499 | SIGNOR-250354 |
P69891 | P17509 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.2 | HOXB6 protein represses globin transcript levels in stably transfected K562 cells in a DNA-binding dependent fashion. | SIGNOR-261638 |
Q9Y2W1 | P21127 | 0 | phosphorylation | up-regulates activity | 0.206 | In addition, genetic knockout of CLK1 or chemical inhibition in mice ameliorated diet-induced obesity and insulin resistance at 22\u00b0C. Through proteomics, we uncovered thyroid hormone receptor-associated protein 3 (THRAP3) as an interacting partner of CLK1, further confirmed by co-immunoprecipitation assays.|We fur... | SIGNOR-280209 |
Q4G163 | Q13555 | 0 | phosphorylation | down-regulates activity | 0.2 | Activated CaMKII and polo-like kinase simultaneously phosphorylate and inactivate Emi2 [ xref , xref , xref , xref , xref ]). | SIGNOR-280200 |
O95817 | Q8NEZ5 | 2 | binding | down-regulates quantity by destabilization | 0.2 | We further demonstrated BAG3, a HSP70 co-chaperone, is a bona fide substrate of SCFFBXO22. FBXO22 mediates BAG3 ubiquitination and degradation that requires ERK-dependent BAG3 phosphorylation at S377. | SIGNOR-277319 |
P06493 | Q9UPU5 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.2 | Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604. | SIGNOR-275605 |
P17676 | P11161 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.534 | Ectopic expression of krox20 can transactivate the c/ebpbeta promoter and increase c/ebpbeta gene expression in 3t3-l1 preadipocytes | SIGNOR-139292 |
P25021 | P08754 | 2 | binding | up-regulates activity | 0.25 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257162 |
P24941 | P38432 | 1 | phosphorylation | up-regulates | 0.381 | In particular, we have recently found that the cdk2/cyclin e complex can phosphorylate coilin in vitro . there is but a single consensus cdk2/cyclin e phosphorylation site in coilin, located at serine 184. when serine 184 was mutated to an alanine (s184a), mimicking a dephosphorylated state, a nucleolar mislocalization... | SIGNOR-84949 |
P48067-2 | P17252 | 0 | phosphorylation | down-regulates activity | 0.332 | We demonstrated that the isoforms GlyT1a, GlyT1b, and GlyT1c were constitutively phosphorylated, and that phosphorylation was dramatically enhanced, in a time dependent fashion, after PKC activation by phorbol ester. The phosphorylation was PKC-dependent, since pre-incubation of the cells with bisindolylmaleimide I, a ... | SIGNOR-262919 |
O15105 | Q15750 | 2 | binding | down-regulates | 0.58 | Smad6 interacts with tak1 and tab1, and smad7 with tab1. The interaction of i-smads with tak1 and/or tab1 implies that several mechanisms exist underlying the repression of the tak1-p38 kinase pathway by i-smads. | SIGNOR-112645 |
O94925 | Q9NXA8 | 2 | binding | up-regulates activity | 0.449 | Immunoprecipitation assays of interaction between GLS and SIRT5 in HepG2 cells infected with lentivirus containing empty or BAG3 construct. Ectopic BAG3 expression decreases the interaction between GLS and SIRT5. It has been reported that SIRT5 is responsible for desuccinylation of GLS35, immunoprecipitation (IP) was ... | SIGNOR-261207 |
O75874 | P36956 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.282 | IDH1 gene transcription is sterol regulated and activated by SREBP-1a and SREBP-2 in human hepatoma HepG2 cells|evidence that IDH1 may regulate lipogenesis in hepatic cells | SIGNOR-253132 |
Q9UK22 | Q9UNE7 | 2 | binding | up-regulates activity | 0.59 | Through this novel interaction, which is mediated by the TPR domain of CHIP and an N-terminal PEST domain of Fbx2, CHIP facilitates the ubiquitination and degradation of Fbx2-bound glycoproteins. This study highlights a novel mechanism of F-box protein-mediated ubiquitination that contributes to glycoprotein homeostasi... | SIGNOR-271589 |
P36952 | P10275 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.401 | In addition, androgen receptor (AR) can recognize and bind to the ARE element, and then inhibit the activity of maspin promoter | SIGNOR-253685 |
Q93038 | O95150 | 2 | binding | up-regulates | 0.776 | The ligand of dr3 is tl1a | SIGNOR-103078 |
Q86Y13 | Q93077 | 1 | monoubiquitination | up-regulates activity | 0.2 | 2A-HUB catalyzes monoubiquitination of H2A at lysine 119, functioning as a combinatoric component of the repression machinery required for specific gene regulation programs. Thus, 2A-HUB mediates a selective repression of a specific set of chemokine genes in macrophages, critically modulating migratory responses to TL... | SIGNOR-271759 |
P24468 | P10589 | 2 | binding | up-regulates | 0.257 | Arp-1/rxr, coup-tfi/rxr, and arp-1/coup-tfi heterodimers bound the fp330-3' site. | SIGNOR-79443 |
Q8IUX7 | P28482 | 0 | phosphorylation | up-regulates activity | 0.2 | We show that DNA binding by AEBP1 requires both the N- and C-terminal domains of AEBP1, and MAPK interaction with AEBP1 (through its N terminus) results in enhanced DNA binding. A threonine at position 623 within the C-terminal domain of AEBP1 plays an important role in DNA binding by AEBP1, because the mutation result... | SIGNOR-262897 |
P01116 | P12931 | 0 | phosphorylation | up-regulates activity | 0.658 | Src phosphorylation promotes the intrinsic exchange rate of KRAS Q61H.|Wild-type and Q61H-mutant KRAS are both phosphorylated by Src on Tyr32 and Tyr64 and dephosphorylated by SHP2, however, SHP2i does not reduce ERK phosphorylation in KRAS Q61H cells. | SIGNOR-278990 |
Q14393 | Q12857 | 0 | transcriptional regulation | down-regulates quantity | 0.2 | By integrating transcriptomic profiling (RNA-seq) of Nfia- and Nfix-deficient GNPs with epigenomic profiling (ChIP-seq against NFIA, NFIB and NFIX, and DNase I hypersensitivity assays), we reveal that these transcription factors share a large set of potential transcriptional targets, suggestive of complementary roles f... | SIGNOR-268876 |
Q8TDY4 | P00533 | 0 | phosphorylation | up-regulates activity | 0.2 | How ACAP4 regulates membrane dynamics and curvature in response to EGF stimulation is unknown. Here, we show that phosphorylation of the N-terminal region of ACAP4, named the Bin, Amphiphysin, and RSV161/167 (BAR) domain, at Tyr34 is necessary for EGF-elicited membrane remodeling. |EGF stimulation of cells causes phosp... | SIGNOR-272234 |
Q04864 | O14920 | 0 | phosphorylation | up-regulates activity | 0.62 | We are the first to identify Ser484 and Ser494 as the major sites of in vitro phosphorylation of REL by IKKalpha and IKKbeta. | SIGNOR-279620 |
P42345 | P51397 | 1 | phosphorylation | down-regulates activity | 0.395 | A critical step in autophagy induction comprises the inactivation of a key negative regulator of the process, the Ser/Thr kinase mammalian target of rapamycin (mTOR). Here we identify death-associated protein 1 (DAP1) as a novel substrate of mTOR that negatively regulates autophagy. Mapping of the phosphorylation sites... | SIGNOR-259812 |
Q92793 | Q13351 | 1 | acetylation | up-regulates activity | 0.49 | EKLF residues acetylated by CREB binding protein (CBP) in vitro map to Lys-288 in its transactivation domain and Lys-302 in its zinc finger domain. | SIGNOR-251826 |
P19086 | Q96LB2 | 2 | binding | up-regulates activity | 0.2 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257326 |
P31321 | P17612 | 2 | binding | down-regulates activity | 0.856 | Inactive PKA exists as a holoenzyme, comprised of two regulatory (R) subunits and two catalytic subunits . In the presence of cAMP, the holoenzyme becomes active by binding two cAMP molecules cooperatively to each R subunit, resulting in a conformational change in the R subunits, thus releasing the two C subunits to ph... | SIGNOR-258753 |
Q14331 | Q4FZB7 | 2 | binding | down-regulates activity | 0.2 | Here we show that, when over-expressed, FRG1 binds and interferes with the activity of the histone methyltransferase Suv4-20h1 both in mammals and Drosophila. Accordingly, FRG1 over-expression or Suv4-20h1 knockdown inhibits myogenesis. | SIGNOR-266639 |
P69905 | P09601 | 0 | null | down-regulates activity | 0.253 | Heme oxygenase (HO), by catabolizing heme to bile pigments, down-regulates cellular hemoprotein, hemoglobin, and heme | SIGNOR-251813 |
Q13428 | P68400 | 0 | phosphorylation | up-regulates activity | 0.305 | Phosphorylated Thr 210 in Treacle is the major interaction site for NBS1|A purified GST fragment of this region was efficiently phosphorylated by CK2 in vitro (Supplementary Fig. 4; T-2) and this fragment pulled down the MRN complex from Hela nuclear extracts only when previously phosphorylated by CK2 | SIGNOR-265086 |
P54132 | Q13315 | 0 | phosphorylation | up-regulates | 0.775 | Mitotic phosphorylation of blm was partially dependent on atm, and phosphorylation sites on blm were identified. A phosphospecific antibody against one of these sites (thr-99) revealed radiation-induced phosphorylation, which was defective in ataxia-telangiectasia cells. These data suggest that atm and blm function tog... | SIGNOR-88010 |
Q06609 | P42574 | 0 | cleavage | down-regulates quantity by destabilization | 0.466 | The RAD51 protein has been shown to be a substrate for caspase-3|he activated caspase-3 fragments (19 kDa and 17 kDa) and caspase-3 cleaved RAD51 fragment (∼23 kDa) was detected by Western analysis (Figure 3E). Activation of caspase-3 and the signature proteolytic degradation product of RAD51 only occurred in parental ... | SIGNOR-271709 |
P43088 | P63092 | 2 | binding | up-regulates activity | 0.373 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256810 |
Q9UBL3 | O43435 | 2 | binding | up-regulates activity | 0.309 | Tbx1 interacts with Ash2l in both yeast and mammalian cells and Ash2l acts as a transcriptional co-activator in luciferase reporter assays. | SIGNOR-251868 |
Q13526 | P01106 | 2 | binding | up-regulates | 0.573 | Pin1 prolyl isomerase enhances recruitment of serine 62-phosphorylated myc and its coactivators to select promoters during gene activation. | SIGNOR-202134 |
Q96GD4 | Q12834 | 1 | phosphorylation | down-regulates activity | 0.767 | When SAC is active, Aurora kinase B (AurkB) phosphorylates and inactivates CDC20, to prevent the activation of anaphase promoting complex and cyclosome (APC/C).|When SAC is active, Aurora kinase B (AurkB) phosphorylates and inactivates CDC20, to prevent the activation of anaphase-promoting complex/cyclosome (APC/C) (Ha... | SIGNOR-280190 |
P00533 | Q99527 | 2 | binding | up-regulates | 0.389 | Gpcr-mediated transactivation of egfrs by estrogen provides a previously unappreciated mechanism of cross-talk between estrogen and serum growth factors, and explains prior data reporting the egf-like effects of estrogen | SIGNOR-115988 |
P0C6X7-PRO_0000037310 | P11362 | 0 | chemical inhibition | down-regulates activity | 0.2 | Pyrimidine 13 showed good potency against all the human VEGFR receptors with an IC50 of 10, 30, and 47 nM for VEGFR-1, -2, and -3, respectively. Significant activity was also seen against the closely related tyrosine receptor kinases PDGFRβ, c-Kit, FGF-R1, and c-fms with IC50’s of 84, 74, 140, and 146 nM, respectively. | SIGNOR-260150 |
P06493 | O00571 | 1 | phosphorylation | down-regulates | 0.297 | Thr204 to glu204 ddx3 mutant protein lost its function, suggesting that phosphorylation at thr204 affects ddx3 function. Thr204 was phosphorylated by cyclin b/cdc2. Thr323 in motif ib was also phosphorylated by cyclin b/cdc2 kinase. We propose a novel function of cyclin b/cdc2 kinase in mitosis, which is to cause a los... | SIGNOR-141569 |
P40189 | P23458 | 1 | null | up-regulates | 0.676 | Il-6 family members typically signal through the common gp130 receptor, with the janus kinase/signal transducer and activator of transcription (jak/stat) pathway being the major intracellular mediator of their effects. | SIGNOR-202036 |
Q12965 | Q05193 | 2 | binding | up-regulates activity | 0.333 | We describe binding of two PRD-containing endocytic proteins, dynamin and synaptojanin-1, to the SH3 domain of Myo1E. This interaction was detected both in vitro, using pull-downs of purified proteins, and in vivo, using immunoprecipitation of protein complexes from synapse-enriched brain extract and immunolocalization... | SIGNOR-265424 |
P15391 | P27986 | 2 | binding | up-regulates activity | 0.585 | CD19 has an extracellular region containing two C2-type Ig-like domains and a cytoplasmic region of ~240 amino acids with 9 conserved tyrosine residues24. Lyn, a Src-family protein tyrosine kinase member, is the dominant kinase that phosphorylates CD19 upon stimulation. Once tyrosyl-phosphorylated, CD19 serves as a mem... | SIGNOR-242900 |
Q14289 | O15259 | 1 | phosphorylation | up-regulates activity | 0.461 | Pyk2 Induces Tyrosine Phosphorylation of NPHP1 at Tyr 46, Tyr 349, and Tyr 721.|The expression of wild-type Pyk2 enhances the amount of co-precipitating PACS-1 with wild-type NPHP1 compared with the presence of the kinase-dead variant of Pyk2. | SIGNOR-278272 |
O60711 | P07948 | 0 | phosphorylation | up-regulates activity | 0.375 | Of a total of 11 tyrosine sites in LPXN, we mutated Tyr(22), Tyr(72), Tyr(198), and Tyr(257) to phenylalanine and demonstrated that LPXN was phosphorylated by Lyn only at Tyr(72) and that this tyrosine site is proximal to the LD3 domain. We further show that LPXN suppressed the secretion of interleukin-2 by BCR-activat... | SIGNOR-262892 |
Q9ULU4 | Q9ULU4 | 2 | binding | up-regulates activity | 0.2 | We identified ZMYND8 as a transcriptional corepressor of the H3K4 demethylase JARID1D|Co-immunoprecipitation between ectopically expressed FLAG-tagged JARID1D and endogenous ZMYND8 protein. | SIGNOR-262037 |
Q05516 | P24941 | 0 | phosphorylation | down-regulates | 0.282 | Here we show that the main cyclin-dependent kinase involved at the g(1) to s transition (cdk2) phosphorylates plzf at two consensus sites found within pest domains present in the hinge region of the protein. This phosphorylation triggers the ubiquitination and subsequent degradation of plzf, which impairs plzf transcri... | SIGNOR-160630 |
P21333 | Q13153 | 0 | phosphorylation | up-regulates | 0.789 | In flna, the pak1-binding site involves tandem repeat 23 in the carboxyl terminus and phosphorylation takes place on serine 2152. | SIGNOR-92065 |
O15034 | Q86UR5 | 2 | binding | down-regulates activity | 0.501 | SH3 domains of RBPs interact with RIMs. The enhancement of depolarization-induced secretion in PC12 cells by fusion proteins that suppress the associations of RBPs with RIMs and α1 suggests that RBPs may repress RIMs, either directly or through associated proteins. | SIGNOR-264361 |
P27707 | Q13535 | 0 | phosphorylation | up-regulates activity | 0.2 | Since activation of dCK by IR was not prevented by KU-60019 at longer times, but could be suppressed if the ATR inhibitor was combined with the ATM inhibitor, we propose that dCK is activated by ATR when ATM is inhibited.|Taken together, these data indicate that ATR, like ATM [15], can directly phosphorylate dCK at Ser... | SIGNOR-279494 |
P25963 | P03186 | 0 | deubiquitination | down-regulates activity | 0.2 | In the current study, we have found that BPLF1 interferes with innate immune activation by targeting multiple intermediates along the TLR signal transduction pathway, including TRAF6, NEMO, and IκBα. BPLF1 can remove ubiquitin tags from proteins in the TLR signaling cascade. This inhibits TLR signaling and decreases th... | SIGNOR-266744 |
Q9UQB8 | P54646 | 0 | phosphorylation | down-regulates | 0.2 | Using this approach for ppp1r12c, baiap2, and cdc27, we found that mutation of a single serine to alanine (s452, s366, and s379 respectively) resulted in almost a complete loss of ampk phosphorylation in these proteins. Termination of irsp53 function is suggested to occur following cdc42 dissociation, kinase phosphoryl... | SIGNOR-195102 |
Q15669 | P43403 | 2 | binding | up-regulates activity | 0.458 | These findings suggest that RhoH is a critical regulator of thymocyte development and TCR signaling by mediating recruitment and activation of Zap70. | SIGNOR-259084 |
P52565 | P16591 | 0 | phosphorylation | down-regulates activity | 0.2 | Fer interferes with binding of RhoGDI\u03b1 and Rac. (A) GST-RhoGDI\u03b1 was pre-incubated with Rac, after which GST-Fer (G-Fer) or GST (G) was added and GST fusion proteins were pulled down with glutathione agarose, followed by kinase reactions. (B) GST-RhoGDI\u03b1 was pre-incubated with GST-Fer or GST in kinase buf... | SIGNOR-279042 |
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