IdA stringlengths 6 21 | IdB stringlengths 6 21 | labels int64 0 2 | mechanism stringclasses 40
values | effect stringclasses 10
values | score float64 0.1 0.99 ⌀ | sentence stringlengths 10 1.63k ⌀ | signor_id stringlengths 12 14 |
|---|---|---|---|---|---|---|---|
Q9UPR3 | Q9BY41 | 2 | binding | up-regulates quantity by stabilization | 0.642 | Here, we report that the human ortholog of the yeast ever-shorter telomeres 1B (EST1B) binds HDAC8. This interaction is regulated by protein kinase A-mediated HDAC8 phosphorylation and protects human EST1B (hEST1B) from ubiquitin-mediated degradation. | SIGNOR-272650 |
P16144 | Q99704 | 2 | binding | down-regulates activity | 0.2 | Integrins also bind to many PTBdomain-containing proteins (Calderwood et al., 2003) – including Dok1 and integrincytoplasmic-domain-associated protein 1 (ICAP1) – and these can compete with talin for binding to integrin and so can impair activation | SIGNOR-257689 |
P14136 | Q9UQM7 | 0 | phosphorylation | down-regulates activity | 0.427 | On the other hand, GFAP was phosphorylated to approximately 1.9 mol of phosphate/mol of GFAP by Ca(2+)-CaM-dependent protein kinase II, and this phosphorylation did induce disassembly of the filament. Sequential analysis of the purified phosphopeptides revealed that only Ser8 on GFAP was phosphorylated by cdc2 kinase, ... | SIGNOR-250626 |
Q9Y5I2 | Q9Y5G5 | 2 | binding | up-regulates activity | 0.2 | The clustered protocadherins comprise the largest subfamily of the cadherin superfamily and are predominantly expressed in the nervous system. Pcdh-alpha proteins interact with beta1-integrin to promote cell adhesion. They also form oligomers with Pcdh-gamma proteins at the same membrane sites. | SIGNOR-265702 |
P04899 | P61073 | 2 | binding | up-regulates activity | 0.484 | Using this model, we have reported that CXCL12 activates Gi1, Gi2, or Gi3 heterotrimeric G proteins in a concentration-dependent manner | SIGNOR-278103 |
Q5SQI0 | Q6PEY2 | 1 | acetylation | up-regulates quantity by stabilization | 0.267 | Alpha-Tubulin acetyltransferase (alphaTAT1) is the major α-tubulin lysine-40 (K40) acetyltransferase in mammals, nematodes, and protozoa, and its activity plays a conserved role in several microtubule-based processes.|The tubulin subunits of microtubules are acetylated, and lysine-40 (K40) of the alpha-tubulin subunit ... | SIGNOR-272248 |
Q12857 | Q9Y6N7 | 1 | transcriptional regulation | up-regulates quantity | 0.2 | For example, within the NFI targetome, we identified 6 collagen genes, 13 genes encoding potassium channel or glutamate receptor subunits and a range of factors related to axon guidance (e.g. Slit1, Robo1, Epha4, Epha5, Epha8) | SIGNOR-268893 |
P49840 | Q9NZQ7 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.2 | We find EGFR inhibitors promote PD-L1 ubiquitination and proteasomal degradation following GSK3α-mediated phosphorylation of Ser279/Ser283. We identify ARIH1 as the E3 ubiquitin ligase responsible for targeting PD-L1 to degradation. | SIGNOR-277552 |
Q9Y5G8 | Q9Y5I2 | 2 | binding | up-regulates activity | 0.2 | The clustered protocadherins comprise the largest subfamily of the cadherin superfamily and are predominantly expressed in the nervous system. Pcdh-alpha proteins interact with beta1-integrin to promote cell adhesion. They also form oligomers with Pcdh-gamma proteins at the same membrane sites. | SIGNOR-265717 |
P48058 | P17612 | 0 | phosphorylation | up-regulates | 0.429 | We found that pka phosphorylation of the ampa receptor subunits glur4 and glur1 directly controlled the synaptic incorporation of ampa receptors in organotypic slices from rat hippocampus. | SIGNOR-97550 |
Q96G30 | P32245 | 2 | binding | down-regulates activity | 0.504 | We report that MRAP and MRAP2 can interact with all 5 MCRs. This interaction results in MC2R surface expression and signaling. In contrast, MRAP and MRAP2 can reduce MC1R, MC3R, MC4R, and MC5R responsiveness to [Nle4,D-Phe7]alpha-melanocyte-stimulating hormone (NDP-MSH). MRAP and MRAP2 can reduce the surface expression... | SIGNOR-252363 |
Q9H0E2 | Q9NPH3 | 2 | binding | down-regulates activity | 0.638 | Binding of IL-1 to its receptor results in rapid assembly of a membrane-proximal signalling complex that consists of two different receptor chains (IL-1Rs), IL-1RI and IL-1RAcP, the adaptor protein MyD88, the serine/threonine kinase IRAK and a new protein, which we have named Tollip. Here we show that, before IL-1β tre... | SIGNOR-251979 |
Q8IV61 | Q04759 | 0 | phosphorylation | up-regulates | 0.328 | Activation of rasgrp3 by phosphorylation of thr-133 is required for b cell receptor-mediated ras activation. our data suggest that pkc, after being activated by diacylglycerol, phosphorylates rasgrp3, thereby contributing to its full activation. | SIGNOR-130490 |
Q9Y6Y9 | P09429 | 2 | binding | up-regulates activity | 0.63 | Here we demonstrate that the extracellular TLR4 adaptor, myeloid differentiation factor 2 (MD-2), binds specifically to the cytokine-inducing disulfide isoform of HMGB1, to the exclusion of other isoforms. Using MD-2-deficient mice, as well as MD-2 silencing in macrophages, we show a requirement for HMGB1-dependent TLR... | SIGNOR-252058 |
Q9HCX4 | Q13976 | 0 | phosphorylation | up-regulates activity | 0.2 | In vitro and in vivo kinase assays have revealed that cGK-Iα phosphorylates mouse TRPC7 but not mouse TRPC3. Site-directed mutagenesis analysis revealed that TRPC7 was phosphorylated by cGK-Iα at threonine 15. Phosphorylation of TRPC7 significantly suppressed carbachol-induced calcium influx and CREB phosphorylation. ... | SIGNOR-263184 |
Q9BXM7 | P62987 | 1 | phosphorylation | up-regulates | 0.385 | Here we report that ubiquitin is the genuine substrate of PINK1. PINK1 phosphorylated ubiquitin at Ser 65 both in vitro and in cells, and a Ser 65 phosphopeptide derived from endogenous ubiquitin was only detected in cells in the presence of PINK1 and following a decrease in mitochondrial membrane potential. | SIGNOR-270342 |
P01266 | P27695 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.2 | In co-transfection experiments, Ref-1 increases the Pax-8 activating effect on thyroglobulin promoter. | SIGNOR-271694 |
O15273 | P49841 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.2 | GSK-3beta phosphorylates FBXL21 and TCAP to activate FBXL21-mediated, phosphodegron-dependent TCAP degradation.|These results show direct GSK-3beta phosphorylation of TCAP S157 and FBXL21 T33 sites. | SIGNOR-264852 |
Q13464 | P61586 | 2 | binding | up-regulates activity | 0.81 | Planar cell polarity (pcp) signalling triggers activation of the small gtpases rhoa and rac1, which in turn activate rho kinase (rock) and jun-n-terminal kinase (jnk), respectively, leading to actin polymerization and microtubule stabilization. | SIGNOR-199542 |
P12830 | Q15139 | 0 | phosphorylation | up-regulates | 0.451 | Our study has identified e-cadherin as a novel substrate of pkd1, and phosphorylation of e-cadherin by pkd1 is associated with increased cellular aggregation and decreased cellular motility in prostate cancer. | SIGNOR-133856 |
O43896 | Q9NRW1 | 1 | relocalization | up-regulates quantity | 0.2 | Here, we identify Bicaudal-D-related protein 1 (BICDR-1) as an effector of the small GTPase Rab6 and key component of the molecular machinery that controls secretory vesicle transport in developing neurons. BICDR-1 interacts with kinesin motor Kif1C, the dynein/dynactin retrograde motor complex, regulates the pericentr... | SIGNOR-266878 |
Q9HD90 | O00712 | 0 | transcriptional regulation | up-regulates quantity | 0.2 | For example, within the NFI targetome, we identified 6 collagen genes, 13 genes encoding potassium channel or glutamate receptor subunits and a range of factors related to axon guidance (e.g. Slit1, Robo1, Epha4, Epha5, Epha8) | SIGNOR-268898 |
Q12913 | P08581 | 1 | dephosphorylation | down-regulates activity | 0.608 | When co-expressed in 293 cells, the full-length substrate-trapping mutant form of DEP-1 formed a stable complex with the chimeric receptor colony stimulating factor 1 (CSF)-Met and wild type DEP-1 dephosphorylated CSF-Met. Furthermore, we observed that DEP-1 preferentially dephosphorylated a Gab1 binding site (Tyr(1349... | SIGNOR-248702 |
O14641 | O14641 | 2 | binding | up-regulates activity | 0.2 | Dix domain of dvl2 mediates dynamic polymerization, which is essential for the signaling activity of dvl2. | SIGNOR-155224 |
P27361 | P35236 | 1 | phosphorylation | up-regulates activity | 0.693 | First, Erk phosphorylates HePTP at residues Thr45 and Ser72. Second, HePTP dephosphorylates Erk at PTyr185.| | SIGNOR-249475 |
Q13315 | P20823 | 1 | phosphorylation | up-regulates | 0.256 | Serine 249 phosphorylation by atm protein kinase regulates hepatocyte nuclear factor-1_ transactivation | SIGNOR-205087 |
P06241 | P11912 | 1 | phosphorylation | up-regulates activity | 0.596 | Lyn and Fyn phosphorylated the CD79a cytoplasmic portion of the fusion proteins well, with >80% of phosphorylation occurring at Y182. CD79a and CD79b function as transducers of B cell antigen receptor signals via a cytoplasmic sequence, termed the immunoreceptor tyrosine-based activation motif (ITAM). | SIGNOR-251153 |
Q9BT67 | P46934 | 1 | relocalization | up-regulates activity | 0.589 | Ndfip1 is primarily localized in the Golgi apparatus where it recruits Nedd4-2 to mediate the degradation of mature hERG proteins during channel trafficking to the plasma membrane. Although Ndfip2 directs Nedd4-2 to the Golgi apparatus, it also recruits Nedd4-2 to the multivesicular bodies (MVBs), which may impair MVB ... | SIGNOR-260997 |
Q92769 | Q5VUA4 | 2 | binding | up-regulates activity | 0.329 | A central domain of TZF is required for repression of AR-mediated transactivation. The results revealed that HDAC2 was coimmunoprecipitated with TZF (Fig. 6A), These results indicate that AR, TZF and HDAC2 form a ternary complex during the repression of AR-mediated transactivation. | SIGNOR-261188 |
P43119 | P38405 | 2 | binding | up-regulates activity | 0.25 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256949 |
Q9H3R0 | P10275 | 2 | binding | up-regulates activity | 0.2 | JMJD2C was found to be co-localized with AR and LSD1 in the epithelium of prostate carcinoma and normal prostate cells. For the detailed mechanism, JMJD2C, AR and LSD1 assembled on the chromatin to remove the methyl groups from mono-, di- and trimethylated H3K9. Importantly, JMJD2C specifically removed the demethylatio... | SIGNOR-263879 |
P09341 | P17612 | 2 | binding | down-regulates | 0.2 | As pka suppresses the activity of gli, smo might use the stimulation of pi3k by galfai and gbetagamma subu- nits to block pka in cells that have high levels of camp. | SIGNOR-152594 |
Q03113 | P32238 | 2 | binding | up-regulates activity | 0.25 | Our studies indi-cate that CCK-A receptors inserted into NIH3T3 cellsalso activate RhoA through G12/13 | SIGNOR-278063 |
P03209 | Q14653 | 1 | transcriptional regulation | down-regulates quantity by repression | 0.2 | EBV Rta selectively down-regulates the expression of IRF3 and IRF7, the main regulators of the Type I IFNs. | SIGNOR-266644 |
Q53ET0 | Q9Y2K2 | 0 | phosphorylation | down-regulates activity | 0.635 | We found that QSK and SIK phosphorylated TORC2 at Ser171 as well as at least two additional residues, namely Ser70 and Ser348|QIK also phosphorylates the CREB co-activator TORC2, in unstimulated cells, to sequester it in the cell cytoplasm, thereby inhibiting CREB-dependent gene-expression | SIGNOR-249170 |
P42681 | Q13094 | 1 | phosphorylation | up-regulates | 0.728 | Resting lymphocyte kinase (rlk/txk) targets lymphoid adaptor slp-76 in the cooperative activation of interleukin-2 transcription in t-cells. In this study, we report that rlk phosphorylates slp-76 at its n-terminal yesp/yepp sites. A third tyrosine within the amino-terminal region (y145) appears to be the most importan... | SIGNOR-44669 |
P04637 | Q07812 | 2 | binding | up-regulates | 0.753 | P53 also accumulates in the cytoplasm where it directly activates bax to promote mitochondrial outer membrane permeabilization. | SIGNOR-140242 |
P33981 | P21589 | 1 | phosphorylation | up-regulates activity | 0.2 | Intermolecular NTE phosphorylation by Mps1 requires a topology in which sites proximal to the NTE interact with the active Mps1 in order to promote phosphorylation. | SIGNOR-280157 |
O75030 | P20265 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.437 | We further demonstrate that BRN2 induces MITF transcription through a binding site located at 50/36 of the MITF promoter | SIGNOR-249616 |
P08247 | Q05193 | 2 | binding | up-regulates activity | 0.333 | The GTPase dynamin I is required for synaptic vesicle (SV) endocytosis. Our observation that dynamin binds to the SV protein synaptophysin in a Ca2+-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. | SIGNOR-264119 |
O43524 | P27361 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.595 | Phosphorylation of foxo3a by erk1/2 at residues ser 294, ser 344 and ser 425 increases foxo3amdm2 interaction and enhances foxo3a degradation via an mdm2-dependent ubiquitin-proteasome pathway | SIGNOR-184569 |
P18564 | O14713 | 2 | binding | down-regulates activity | 0.287 | Integrins also bind to many PTBdomain-containing proteins (Calderwood et al., 2003) – including Dok1 and integrincytoplasmic-domain-associated protein 1 (ICAP1) – and these can compete with talin for binding to integrin and so can impair activation | SIGNOR-257662 |
Q9BQQ3 | P28482 | 0 | phosphorylation | down-regulates activity | 0.27 | Here we show that GRASP65 is phosphorylated on serine 277 in interphase cells, and this is strongly enhanced in response to the addition of serum or epidermal growth factor. This is directly mediated by ERK suggesting that GRASP65 has some role in growth factor signal transduction. These results argue against Ser-277 p... | SIGNOR-262841 |
Q16875 | Q9P1W9 | 0 | phosphorylation | up-regulates quantity by stabilization | 0.2 | We used biochemical methods to determine that PIM2 can directly bind and change the phosphorylation of PFKFB3 at Ser478 to enhance PFKFB3 protein stability through the ubiquitin-proteasome pathway. | SIGNOR-277554 |
P08559 | P54646 | 0 | phosphorylation | up-regulates activity | 0.2 | AMPKα phosphorylates PDHA subunit on Ser295 and Ser314 to activate PDH complex | SIGNOR-276838 |
O75030 | P16220 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.633 | Therefore, the molecular steps linking cAMPto melanogenesis up-regulation appear currently better elucidated. cAMP activates PKA, and PKA phosphorylates and activates CREB which, when activated, binds to the CRE domain present in the microphthalmia promoter,thereby up-regulating its transcription. | SIGNOR-249619 |
O00327 | P37231 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.399 | Rosiglitazone treatment induced aortic expression of Bmal1 mRNA, and ChIP and promoter assays revealed that Bmal1 is a direct PPARgamma target gene. These studies have uncovered a role for vascular PPARgamma as a peripheral factor participating in regulation of cardiovascular rhythms. | SIGNOR-268026 |
P31749 | P98170 | 1 | phosphorylation | up-regulates quantity by stabilization | 0.618 | Akt, including akt1 and akt2, interacts with and phosphorylates x-linked inhibitor of apoptosis protein (xiap) at residue serine-87 in vitro and in vivo. Phosphorylation of xiap by akt protects xiap from ubiquitination and degradation in response to cisplatin. | SIGNOR-119488 |
Q15276 | P20339 | 2 | binding | up-regulates | 0.928 | We have previously shown that rab5, which regulates various steps of transport along the early endocytic pathway, is activated by a complex consisting of rabex-5, a rab5 nucleotide exchange factor, and the effector rabaptin-5. | SIGNOR-109352 |
Q9HC56 | P05556 | 2 | binding | up-regulates activity | 0.2 | The clustered protocadherins comprise the largest subfamily of the cadherin superfamily and are predominantly expressed in the nervous system. Pcdh-alpha proteins interact with beta1-integrin to promote cell adhesion. | SIGNOR-269035 |
O95837 | O60755 | 2 | binding | up-regulates activity | 0.289 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257206 |
P17181 | P01574 | 2 | binding | up-regulates | 0.867 | Ifn-alpha, ifn-beta, and ifn-omega, induce somewhat different cellular effects but act through a common receptor complex, ifnar, composed of subunits ifnar-1 and ifnar-2. | SIGNOR-104663 |
P47710 | Q04206 | 1 | phosphorylation | up-regulates | 0.2 | Phosphorylation of serine 529 of p65 is mediated by casein kinase ii, but is prevented in nonstimulated cells by the interaction with ikba | SIGNOR-171222 |
Q15303 | Q96JA1 | 0 | ubiquitination | down-regulates | 0.608 | We report upregulation of lrig1 transcript and protein upon egf stimulation, and physical association of the encoded protein with the four egfr orthologs of mammals. Upregulation of lrig1 is followed by enhanced ubiquitylation and degradation of egfr. The underlying mechanism involves recruitment of c-cbl, an e3 ubiqui... | SIGNOR-139954 |
Q9HA47 | Q13315 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.2 | ATM also phosphorylates UCK1 at S145, significantly enhancing the KLHL2-UCK1 complex formation|We demonstrated that the ubiquitin E3 ligase KLHL2 interacted with UCK1 and mediated its polyubiquitination at the K81 residue and degradation. | SIGNOR-275963 |
P12931 | P46934-4 | 1 | phosphorylation | up-regulates activity | 0.417 | Activation of c-Src by epidermal growth factor (EGF) also promoted tyrosine phosphorylation and enhanced the activity of NEDD4. | SIGNOR-276860 |
P26599 | P01106 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.438 | We also demonstrate that the oncogenic transcription factor c-Myc upregulates transcription of PTB, hnRNPA1 and hnRNPA2, | SIGNOR-268689 |
P15976 | Q01196 | 2 | binding | up-regulates activity | 0.643 | We and others have previously shown that RUNX1 and GATA-1 physically interact and cooperate in the activation of megakaryocytic promoters such as alpha IIb integrin and glycoprotein Ibalpha. In particular, we will elaborate on recent data which suggest that GATA-1 targets RUNX1 for modification, in particular phosphor... | SIGNOR-254194 |
Q92823 | P54762 | 0 | phosphorylation | up-regulates activity | 0.411 | EphB receptors were found to induce phosphorylation of NrCAM on the tyrosine residue within the FIGQY ankyrin binding motif, inhibiting ankyrin recruitment. Furthermore, NrCAM phospho-FIGQY levels in the SC were decreased in EphB1/3 and EphB1/2/3 null mice and increased in mutant mice overexpressing constitutively acti... | SIGNOR-262862 |
Q16552 | Q9BYH8 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.381 | In cooperation with RORγt and RORα, IκBζ enhances Il17a expression by binding directly to the regulatory region of the Il17a gene. | SIGNOR-266210 |
Q02763 | O95841 | 2 | binding | up-regulates | 0.506 | In experiments with human endothelial cell lines, ang3 was identified as an antagonist of tie2 and ang4 was identified as an agonist of tie2. | SIGNOR-127354 |
O75030 | P28482 | 0 | phosphorylation | down-regulates | 0.705 | The current study reveals that c-kit signaling triggers two phosphorylation events on mi, which up-regulate transactivation potential yet simultaneously target mi for ubiquitin-dependent proteolysis. The specific activation/degradation signals derive from mapk/erk targeting of serine 73the results suggested that s1p re... | SIGNOR-75030 |
O15530 | P31751 | 1 | phosphorylation | up-regulates activity | 0.732 | Akt1 and akt2 are phosphorylated and activated by the protein kinase pdk1 at thr-308 or thr-309, respectively, in the activation t-loop, and further activation occurs through phosphorylation at ser-473 or ser-474, respectively. Pdk1 phosphorylates akt-2 at thr 309 in the catalytic domain, leading to enzymatic activatio... | SIGNOR-134485 |
Q16665 | P46531 | 0 | relocalization | up-regulates activity | 0.649 | The notch intracellular domain interacts with hif-1alpha and hif-1alpha is recruited to notch-responsive promoters upon notch activation under hypoxic conditions. | SIGNOR-141315 |
P06493 | P49821 | 1 | phosphorylation | up-regulates activity | 0.2 | Here, we show that a fraction of cyclin B1/Cdk1 proteins localizes to the matrix of mitochondria and phosphorylates a cluster of mitochondrial proteins, including the complex I (CI) subunits in the respiratory chain. Cyclin B1/Cdk1-mediated CI phosphorylation enhances CI activity, whereas deficiency of such phosphoryla... | SIGNOR-275594 |
P53350 | O15350 | 1 | phosphorylation | down-regulates | 0.51 | P73-mediated transcriptional activity is negatively regulated by polo-like kinase 1. tap73 is phosphorylated by this kinase on threonine-27 (thr-27) within the ta domain. | SIGNOR-178253 |
P21796 | Q96A26 | 2 | binding | up-regulates activity | 0.2 | HGTD-P was coprecipitated with VDAC but not with ANT or cyclophilin D (Fig. 7A, left upper panel).|However, it is not clear at present whether HGTD-P participates directly in channel formation in association with VDAC or modulates its channel-forming activity. | SIGNOR-260293 |
Q13627 | Q05193 | 1 | phosphorylation | down-regulates | 0.416 | Mnb/dyrk1a was shown to phosphorylate dynamin 1 and alter its interactions with several sh3 domain-containing endocytic accessory proteins.Phosphorylation At s795 and s857 was confirmed in full-length dynamin 1, and s857 was subsequently determined to be the major mnb/dyrk1a phosphorylation site in vitro. Phosphorylati... | SIGNOR-127440 |
O00399 | P53350 | 2 | binding | up-regulates activity | 0.374 | Here, we show that the p27/p25 heterodimer undergoes mitotic phosphorylation by cyclin‐dependent kinase 1 (Cdk1) at a single site, p27 Thr186, to generate an anchoring site for polo‐like kinase 1 (Plk1) at kinetochores. | SIGNOR-264798 |
Q99538 | Q6R6M4 | 0 | deubiquitination | down-regulates quantity by destabilization | 0.309 | We demonstrate that TRAF6 ubiquitinates the proform of AEP through K63-linked polyubiquitin, reversible by USP17, and forms a complex with HSP90α to subsequently promote pro-AEP intracellular stability as well as secretion. We now present evidence that AEP is a substrate for TRAF6 ubiquitination, resulting in AEP/TRAF6... | SIGNOR-272854 |
Q05682 | P17252 | 0 | phosphorylation | down-regulates | 0.357 | Phosphorylation of both intact caldesmon and of its c-terminal fragment (658c), containing residues 658-756, significantly decreased their ability to inhibit acto-heavy meromyosin atpase. | SIGNOR-36792 |
O94782 | Q8WYQ5 | 1 | deubiquitination | up-regulates quantity by stabilization | 0.2 | Specifically, radiation-induced ATM-dependent phosphorylation of DGCR8 at serine 677 facilitates USP51 to bind, deubiquitinate, and stabilize DGCR8, which leads to the recruitment of DGCR8 and DGCR8's binding partner RNF168 to MDC1 and RNF8 at DSBs. | SIGNOR-277308 |
P12931 | Q16832 | 1 | phosphorylation | up-regulates | 0.38 | Here, using baculoviral co-expression of the ddr2 cytosolic domain and src, we show that src targets three tyrosine residues (tyr-736, tyr-740, and tyr-741) in the activation loop of ddr2 for phosphorylation. This phosphorylation by src stimulates ddr2 cis-autophosphorylation of additional tyrosine residues. | SIGNOR-140767 |
P14921 | P49841 | 0 | phosphorylation | up-regulates quantity by stabilization | 0.2 | Here, we show that ETS1 forms a complex with glycogen synthase kinase-3β (GSK3β). Specifically, GSK3β-mediated phosphorylation of ETS1 at threonine 265 and serine 269 promoted protein stability, induced the transcriptional activation of matrix metalloproteinase (MMP)-9, and increased cell migration. | SIGNOR-277560 |
O95837 | Q9HBW0 | 2 | binding | up-regulates activity | 0.484 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257431 |
Q5T7P8 | P05771 | 0 | phosphorylation | up-regulates activity | 0.2 | We found by site-directed mutagenesis that Thr418 and/or Thr419 in the polybasic region (KKKTTIK) of the C2B domain--a key region for the function of synaptotagmins--are the PKC target that regulates its inhibitory effect on acrosomal exocytosis. Similarly, we showed that Thr284 in the polybasic region of C2A (KCKLQTR)... | SIGNOR-273565 |
Q96GF1 | Q12981 | 1 | polyubiquitination | up-regulates activity | 0.533 | RNF185 functions as a ubiquitin E3 ligase, enabling BNIP1-p62 interaction. Here we show that human RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy in cultured cells. Human BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based u... | SIGNOR-271931 |
Q00535 | Q9Y696 | 1 | phosphorylation | up-regulates quantity by stabilization | 0.2 | These results confirm that CDK5 phosphorylates CLIC4 at serine 108.|We found that activated CDK5 phosphorylated serine 108 in CLIC4, increasing CLIC4 protein stability, and accumulation. | SIGNOR-279451 |
P01112 | Q5VWQ8 | 0 | gtpase-activating protein | down-regulates activity | 0.6 | The GAP domain of DAB2IP is homologous to other Ras-GAPs, such as GAP120 and neurofibromin (NF1), and can stimulate the GTPase activity of RAS proteins both in vitro and in cancer cell lines. DAB2IP is able to stimulate in vitro and in vivo the GTPase activity of RAS proteins (H-Ras, K-Ras, and N-Ras) facilitating GTP ... | SIGNOR-254745 |
P19793 | P37231 | 2 | binding | up-regulates | 0.743 | Although the three ppar subtypes are closely related and bind to similar dna response elements as heterodimers with the 9-cis retinoic acid receptor rxr, each subserves a distinct physiology | SIGNOR-105445 |
Q5UE93 | P31749 | 0 | phosphorylation | up-regulates activity | 0.45 | P84 forms a negative regulatory complex with p110gamma to control PI3Kgamma signalling during cell migration|However, phosphorylation at this site was confirmed using an in vitro kinase assay in which Akt kinase was shown to readily phosphorylate Thr607 using a p84 peptide (Figure 1e), where Thr607 in conjunction with ... | SIGNOR-275722 |
O15392 | P53350 | 0 | phosphorylation | up-regulates | 0.583 | Thus, we conclude that plk1-mediated phosphorylation of sur at ser20 is critical for accurate chromosome segregation|SUR (survivin) | SIGNOR-170460 |
P62136 | P38398 | 1 | dephosphorylation | down-regulates activity | 0.377 | Protein kinases involved in the DNA damage checkpoint control, such as ATM, ATR, and hCds1/Chk2, have been shown to phosphorylate and activate BRCA1 upon DNA damage. |Altogether, these results indicate that PP1α specifically dephosphorylates BRCA1 at multiple serine sites, including S988 [12], S1423, and S1524. | SIGNOR-248560 |
P15884 | P35222 | 2 | binding | up-regulates activity | 0.69 | beta-catenin interacts with the TCF/Lef family transcription factors. | SIGNOR-178042 |
Q8N726 | Q9UHC7 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.366 | Biochemical analyses confirmed that MKRN1 targets p14ARF for ubiquitination and subsequent proteasome-dependent degradation.The Skp1-Cul1-F-box-protein44 (SCF(FBXO44)) complex ubiquitinates full-length BRCA1 in vitro. | SIGNOR-272036 |
Q99986 | Q12888 | 1 | phosphorylation | up-regulates | 0.399 | The kinase vrk1 is activated by dna double strand breaks induced by ionizing radiation (ir) and specifically phosphorylates 53bp1 in serum-starved cells./ Vrk1 knockdown resulted in the defective formation of 53bp1 foci in response to ir both in number and size | SIGNOR-197625 |
P08069 | Q13191 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.422 | The ubiquitin ligase Cbl-b also ubiquitinated and degraded IGF-IR and inhibited the Akt/ERK-miR-200c-ZEB2 axis, leading to the repression of IGF-I-induced EMT. | SIGNOR-278607 |
P11166 | P27105 | 2 | binding | down-regulates activity | 0.527 | Similar to the results obtained in the RBC, Glut1 and stomatin immunoprecipitated with each other in lysates of Clone 9 cells. The above results suggest that stomatin is closely associated with Glut1 in the plasma membrane and that overexpression of stomatin results in a depression in the basal rate of glucose transpor... | SIGNOR-261278 |
P43354 | P28482 | 0 | phosphorylation | up-regulates | 0.395 | We have shown that erk2 is a kinase to phosphorylate nurr1 on multiple sites. S126 and t132, which are located near af1 core of nurr1, are dominant sites phosphorylated by erk2. reporter gene assays show that nurr1delta124-133/t185a, an erk2 phospho-site mutant form, could not further increase its transcriptional acti... | SIGNOR-157167 |
Q06830 | P06239 | 0 | phosphorylation | down-regulates activity | 0.2 | Inactivation of peroxiredoxin I by phosphorylation allows localized H(2)O(2) accumulation for cell signaling. To determine whether Prxs are phosphorylated, we subjected recombinant human PrxI and II to an in vitro kinase assay with two nonreceptor PTKs, Lck and Abl, in the presence of [γ-32P]ATP. Both PTKs phosphorylat... | SIGNOR-276277 |
P00519 | Q03468 | 1 | phosphorylation | up-regulates activity | 0.271 | N-terminal region of CSB interacts with the SH3 domain of c-Abl in vitro and in vivo. In addition, c-Abl kinase phosphorylates CSB at Tyr932. our results suggest that c-Abl interacts with and tyrosine phosphorylates CSB. This interaction may play an important role in the response to oxidative stress, resulting in activ... | SIGNOR-251933 |
Q8WUJ0 | Q969H0 | 2 | binding | down-regulates activity | 0.346 | STYX acts as a direct inhibitor of FBXW7, affecting the cellular levels of its substrates. Furthermore, we find that levels of STYX and FBXW7 are anti-correlated in breast cancer patients, | SIGNOR-251663 |
P48431 | O00308 | 0 | ubiquitination | down-regulates quantity | 0.361 | Among the four E3 ligases, only WWP2 knockdown was found to increase SOX2 protein levels in GSCs (Fig.\u00a04A).|We first verified that WWP2 ubiquitinates SOX2 in vitro. | SIGNOR-278798 |
P20396 | P23769 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.263 | The rat prepro-TRH gene is activated by GATA2. | SIGNOR-267259 |
Q96HE7 | Q9BS26 | 2 | binding | up-regulates quantity by stabilization | 0.2 | Here, we report the functional characterization of a novel UPR-induced ER resident protein (ERp44) that forms mixed disulfides with both hEROs, as well as with partially unfolded Ig subunits. | SIGNOR-261049 |
P07948 | P31995 | 1 | phosphorylation | up-regulates activity | 0.2 | Fyn and Blk definitely phosphorylate Y-282 in the ITAM of FcgRIIa/c, whereas the non-ITAM tyrosine residue (Y-275) becomes phosphorylated by Syk, as the phosphorylation of double point mutants shows. In addition to these tyrosine residues, Fyn, Blk, and Syk might phosphorylate the most C-terminal tyrosine residue (Y-29... | SIGNOR-262676 |
O14641 | Q96GF1 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.466 | The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation. Overexpression of RNF185 decreases the exogenous and endogenous level of Dvl2, promotes the ubiquitination and degradation of Dvl2 | SIGNOR-272173 |
Q9GZR7 | Q00987 | 0 | polyubiquitination | up-regulates activity | 0.334 | MDM2 mediates nonproteolytic polyubiquitylation of the DEAD-Box RNA helicase DDX24. Unexpectedly, however, the polyubiquitylation of DDX24 did not elicit its proteasomal degradation but rather promoted its association with preribosomal ribonucleoprotein (pre-rRNP) processing complexes that are required for the early st... | SIGNOR-272845 |
Q13131 | Q9P1W9 | 0 | phosphorylation | down-regulates activity | 0.2 | Specifically, we found that PIM2 bound to AMPKα1, and directly phosphorylated it on Thr467. Phosphorylation of AMPKα1 by PIM2 led to decreasing AMPKα1 kinase activity, which in turn promoted aerobic glycolysis and tumor growth. | SIGNOR-277471 |
P24530 | P63096 | 2 | binding | up-regulates activity | 0.449 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257053 |
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