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PMID:24731 | Enhancement by choline of the induction of adrenal tyrosine hydroxylase by phenoxybenzamine, 6-hydroxydopamine, insulin or exposure to cold. | Treatments that increase the release of acetylcholine from the splanchnic nerve have previously been shown to induce the enzyme tyrosine hydroxylase in adrenal chromaffin cells. Such treatments include the systemic administration of the drugs phenoxybenzamine and 6-hydroxydopamine, insulin-induced hypoglycemia, and pro... | Enhancement by choline of the induction of adrenal tyrosine hydroxylase by phenoxybenzamine, 6-hydroxydopamine, insulin or exposure to cold. Treatments that increase the release of acetylcholine from the splanchnic nerve have previously been shown to induce the enzyme tyrosine hydroxylase in adrenal chromaffin cells. S... | [
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PMID:24734 | The increase in spontaneous transmitter release produced by beta-bungarotoxin and its modification by inorganic ions. | The excitatory phase of the biphasic action on transmitter release of the neurotoxin, beta-bungarotoxin (beta-BuTX; 0.5 microgram ml-1), was studied on miniature end-plate potentials (MEPPs) at frog sciatic nerve-sartorius muscle junctions. The most common type of excitatory response was characterized by a continuous i... | The increase in spontaneous transmitter release produced by beta-bungarotoxin and its modification by inorganic ions. The excitatory phase of the biphasic action on transmitter release of the neurotoxin, beta-bungarotoxin (beta-BuTX; 0.5 microgram ml-1), was studied on miniature end-plate potentials (MEPPs) at frog sci... | [
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PMID:24735 | The generation of nerve impulses in mammalian axons by changing the concentrations of the normal constituents of extracellular fluid. | 1. Experiments have been performed to determine what changes in normal constituents of extracellular fluid are necessary to generate action potentials in mammalian axons. 2. Single and multifibre preparations of rat spinal roots were tested with Krebs solutions in which Na+, K+, Ca2+, Mg2+, pH, PCO2 or osmotic pressure... | The generation of nerve impulses in mammalian axons by changing the concentrations of the normal constituents of extracellular fluid. 1. Experiments have been performed to determine what changes in normal constituents of extracellular fluid are necessary to generate action potentials in mammalian axons. 2. Single and m... | [
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PMID:24736 | Calcium and strontium concentration changes within skinned muscle preparations following a change in the external bathing solution. | 1. A method for producing rapid [Ca2+] and [Sr2+] changes in the frog skinned muscle fibre preparation while maintaining constant all other cationic concentrations (Moisescu, 1976a, b) is described and analysed in detail. 2. Different experiments, some of them involving the Ca2+-sensitive photoprotein aequorin, as well... | Calcium and strontium concentration changes within skinned muscle preparations following a change in the external bathing solution. 1. A method for producing rapid [Ca2+] and [Sr2+] changes in the frog skinned muscle fibre preparation while maintaining constant all other cationic concentrations (Moisescu, 1976a, b) is ... | [
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PMID:24737 | The effect of external pH on osmotic permeability, ion and fluid transport across isolated frog skin. | 1. The rate of volume flow across frog skin induced by an osmotic gradient was measured when normal (7.4) and low pH (2.28) solutions bathed the outside. The osmotic permeabilities (Pos) were 2.4 +/- 0.4 and 4.8 +/- 1.0 micrometer/sec, respectively. The change in Pos induced by low pH was reversible. 2. Volume flow in ... | The effect of external pH on osmotic permeability, ion and fluid transport across isolated frog skin. 1. The rate of volume flow across frog skin induced by an osmotic gradient was measured when normal (7.4) and low pH (2.28) solutions bathed the outside. The osmotic permeabilities (Pos) were 2.4 +/- 0.4 and 4.8 +/- 1.... | [
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PMID:24738 | Measurement of pH in the rat epididymis in vivo. | Antimony microelectrodes were used to measure pH in rat seminiferous tubules and epididymides in vivo. Acidification of fluid leaving the testis occurs primarily in the initial segment and to a lesser extent in the intermediate zone. | Measurement of pH in the rat epididymis in vivo. Antimony microelectrodes were used to measure pH in rat seminiferous tubules and epididymides in vivo. Acidification of fluid leaving the testis occurs primarily in the initial segment and to a lesser extent in the intermediate zone. | [
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PMID:24748 | Chloride conductance of the amphiuma red cell membrane. | Like most other red cells, the giant erythrocytes of Amphiuma means possess a system for rapid exchange of chloride across the membrane. Also, there are indications that the net transport of chloride in these cells is slow. The size of Amphiuma erythrocytes allows direct measurements of membrane potential with microele... | Chloride conductance of the amphiuma red cell membrane. Like most other red cells, the giant erythrocytes of Amphiuma means possess a system for rapid exchange of chloride across the membrane. Also, there are indications that the net transport of chloride in these cells is slow. The size of Amphiuma erythrocytes allows... | [
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PMID:24754 | Resistance to Marek's disease. Effect of Corynebacterium parvum and Marek's tumor cell vaccines on tumorigenesis in chickens. | A study of nonspecific stimulation of the avian immune system with Corynebacterium parvum and specific stimulation with Marek's tumor cell vaccines revealed that nonspecifically stimulated outbred White Leghorn-type cockerels had higher incidences of tumors than did controls. A study of tumor cell cytotoxicity of sera ... | Resistance to Marek's disease. Effect of Corynebacterium parvum and Marek's tumor cell vaccines on tumorigenesis in chickens. A study of nonspecific stimulation of the avian immune system with Corynebacterium parvum and specific stimulation with Marek's tumor cell vaccines revealed that nonspecifically stimulated outbr... | [
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PMID:24755 | Traumatic pseudoaneurysm of the dorsalis pedis artery in a child. | A basic principle of children's surgery is to plan it to last for 70 years. The successful reconstruction of the dorsalis pedis artery in an 8-year-old after trauma may be of benefit if he develops peripheral vascular disease as an adult. | Traumatic pseudoaneurysm of the dorsalis pedis artery in a child. A basic principle of children's surgery is to plan it to last for 70 years. The successful reconstruction of the dorsalis pedis artery in an 8-year-old after trauma may be of benefit if he develops peripheral vascular disease as an adult. | [
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PMID:24756 | Multiple endocrine adenomatosis: in support of the common origin theories. | Herein we give clinical support to the theory of a common origin of multiple endocrine adenomatosis. Accurate clinical evaluation of a patient suspected to have endocrine neoplasia requires the search for a much wider range of potential endocrine tumors. Such an approach is now made possible by the radioimmunoassay. Ch... | Multiple endocrine adenomatosis: in support of the common origin theories. Herein we give clinical support to the theory of a common origin of multiple endocrine adenomatosis. Accurate clinical evaluation of a patient suspected to have endocrine neoplasia requires the search for a much wider range of potential endocrin... | [
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PMID:24757 | Anxiety states in general practice. | Anxiety is commonly encountered in patient seen by the general practitioner. Frequently it either stimulates an organic condition involving one or more systems of the body or may aggravate symptoms of already existent organic pathology. Presence of environmental stresses and of personal conflicts in the life history of... | Anxiety states in general practice. Anxiety is commonly encountered in patient seen by the general practitioner. Frequently it either stimulates an organic condition involving one or more systems of the body or may aggravate symptoms of already existent organic pathology. Presence of environmental stresses and of perso... | [
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PMID:24770 | Laboratory and field assessment of the molluscicidal activity of gogo (Entada phaseoloides) against the amphibious snail intermediate host of Schistosoma japonicum. | A molluscicidal fraction occurs naturally in the bark of a vine (gogo in Tagalog), Entada phaseoloides, which grows indigeneously and abundantly in the Philippines. Butanol fraction of the methanol extracts of the bark was most toxic against Oncomelania quadrasi with the LC50 of 3.6-5.8 ppm. Analytical work on the buta... | Laboratory and field assessment of the molluscicidal activity of gogo (Entada phaseoloides) against the amphibious snail intermediate host of Schistosoma japonicum. A molluscicidal fraction occurs naturally in the bark of a vine (gogo in Tagalog), Entada phaseoloides, which grows indigeneously and abundantly in the Phi... | [
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PMID:24776 | Pharmacology of Sch 11973, N-(2-phenylisopropyl)-N-p-toluene sulfonyl urea, a potential new antianginal agent. | Antianginal drugs were evaluated on the basis of their ability to protect against subepicardial electrogram changes induced by local ventricular ischemia in anesthetized dogs. Sch 11973 [N-(2-phenylisopropyl)-N-p-toluene sulfonyl urea], a potential new antianginal agent, was also effective against local ventricular isc... | Pharmacology of Sch 11973, N-(2-phenylisopropyl)-N-p-toluene sulfonyl urea, a potential new antianginal agent. Antianginal drugs were evaluated on the basis of their ability to protect against subepicardial electrogram changes induced by local ventricular ischemia in anesthetized dogs. Sch 11973 [N-(2-phenylisopropyl)-... | [
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PMID:24777 | Involvement of histamine H1- and H2-receptors in induced asthmas in dogs. | Participation of histamine H1- and H2-receptors in both asthmas, i.e. experimentally induced bronchoconstriction and bronchosecretion, with ascaris suum and histamine in anesthetized dogs was investigated. Dogs given 0.2% histamine solution or ascaris antigen (3 mg protein) by inhalation showed increases in respiratory... | Involvement of histamine H1- and H2-receptors in induced asthmas in dogs. Participation of histamine H1- and H2-receptors in both asthmas, i.e. experimentally induced bronchoconstriction and bronchosecretion, with ascaris suum and histamine in anesthetized dogs was investigated. Dogs given 0.2% histamine solution or as... | [
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PMID:24778 | The inhibitory effect of papaverine on respiration-dependent contracture of guinea pig taenia coli in high-K medium. I. The relationship between contracture and respiration. | Papaverine (Pap) inhibited a tonic tension development in guinea pig taenia coli induced by an elevation of the potassium concentration of the medium to 40 mM (40-K). The ID50 of Pap was 4.5 x 10(-6) M. Inhibition of the 40-K induced tonic tension by Pap was dependent on the calculated concentration of non-ionized form... | The inhibitory effect of papaverine on respiration-dependent contracture of guinea pig taenia coli in high-K medium. I. The relationship between contracture and respiration. Papaverine (Pap) inhibited a tonic tension development in guinea pig taenia coli induced by an elevation of the potassium concentration of the med... | [
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PMID:24782 | Intraoperative measurement of lower esophageal spincter pressure. | The lower esophageal sphincter pressure has been measured intraoperatively in 200 patients with gastroesophageal reflux and in three patients with achalasia. Lower esophageal sphincter pressure is measured before and during repair. Calibrating the cardia during performance of the median arcuate posterior gastropexy all... | Intraoperative measurement of lower esophageal spincter pressure. The lower esophageal sphincter pressure has been measured intraoperatively in 200 patients with gastroesophageal reflux and in three patients with achalasia. Lower esophageal sphincter pressure is measured before and during repair. Calibrating the cardia... | [
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PMID:24784 | The mechanism and control of human erythrocyte zinc uptake. | The increasing awareness of the importance of the divalent cation zinc in normal and pathologic cell functions has prompted our investigations into the mechanism and control of human erythrocyte zinc uptake. The albumin in blood plasma appears to be the main zinc binding moiety, effectively limiting zinc availability o... | The mechanism and control of human erythrocyte zinc uptake. The increasing awareness of the importance of the divalent cation zinc in normal and pathologic cell functions has prompted our investigations into the mechanism and control of human erythrocyte zinc uptake. The albumin in blood plasma appears to be the main z... | [
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PMID:24785 | Determinants of lengths of outpatient visits in a prepaid group practice setting. | In this paper we have examined the amount of time that physicians (MDs) and physician's assistants (PAs) expended on office visits. The purpose was to determine the extent to which a series of patient-related and system-related characteristics explain variations in that time. Using regression analyses, we found most of... | Determinants of lengths of outpatient visits in a prepaid group practice setting. In this paper we have examined the amount of time that physicians (MDs) and physician's assistants (PAs) expended on office visits. The purpose was to determine the extent to which a series of patient-related and system-related characteri... | [
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PMID:24797 | Transport of 14C-L-glycine by the haustorial mycoparasite Tieghemiomyces parasiticus in axenic culture. | Transport of L-glycine by the haustorial mycoparasite Tieghemiomyces parasiticus grown in axenic culture was investigated using endogenously metabolizing cells. Transport occurred against a concentration gradient and required the expenditure of energy supplied by metabolic reactions that were inhibited by catalytic amo... | Transport of 14C-L-glycine by the haustorial mycoparasite Tieghemiomyces parasiticus in axenic culture. Transport of L-glycine by the haustorial mycoparasite Tieghemiomyces parasiticus grown in axenic culture was investigated using endogenously metabolizing cells. Transport occurred against a concentration gradient and... | [
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PMID:24798 | New approaches to the pathogenesis of peptic ulcer based on the protective action of saliva with special reference to roughage, vegetable fibre and fermented milk products. | Epidemiologic studies have shown differences in the prevalence of peptic ulcer disease among various communities to be related to variation in diet and eating patterns. Results of studies of the effect of diet and pattern of eating on saliva, on gastric juice, and on the amount of bile are reviewed. It is suggested tha... | New approaches to the pathogenesis of peptic ulcer based on the protective action of saliva with special reference to roughage, vegetable fibre and fermented milk products. Epidemiologic studies have shown differences in the prevalence of peptic ulcer disease among various communities to be related to variation in diet... | [
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PMID:24802 | [Inhibition of electron transport and photophosphorylation in chloroplasts by quercetin]. | The influence of quercetin on electron transport and photophosphorylation of pea isolated chloroplasts with methylviologen and NADP+ has been studied. Quercetin inhibits ATP synthesis and phosphorylating electron transport but does not affect the basal electron transport in the presence of methylviologen. In view of th... | [Inhibition of electron transport and photophosphorylation in chloroplasts by quercetin]. The influence of quercetin on electron transport and photophosphorylation of pea isolated chloroplasts with methylviologen and NADP+ has been studied. Quercetin inhibits ATP synthesis and phosphorylating electron transport but doe... | [
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PMID:24803 | [Equilibrium dynamics of the 3-dimensional structure of globular proteins]. | According to the data of many physical and physico-chemical methods for conditions near to physiological, there are two types of spontaneous reversible conformational transitions in native proteins namely, local transconformations and overall unfolding of the molecule. Conformational transitions of both types occur wit... | [Equilibrium dynamics of the 3-dimensional structure of globular proteins]. According to the data of many physical and physico-chemical methods for conditions near to physiological, there are two types of spontaneous reversible conformational transitions in native proteins namely, local transconformations and overall u... | [
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PMID:24804 | [Stationary kinetics of catalysis by the hydrogenase of Thiocapsa roseopersicina]. | Forward and reverse reactions of catalysis by a hydrogenase from T. roseopersicina in steady state have been investigated. The dependance of reaction rate on the concentration of substrates and of hydrogen ions has been studied. Detailed formal-kinetic analysis of possible mechanisms of enzyme action has been carried o... | [Stationary kinetics of catalysis by the hydrogenase of Thiocapsa roseopersicina]. Forward and reverse reactions of catalysis by a hydrogenase from T. roseopersicina in steady state have been investigated. The dependance of reaction rate on the concentration of substrates and of hydrogen ions has been studied. Detailed... | [
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PMID:24808 | Effect of hypnotic and anxiolytic agents on regional concentration of acetylcholine in rat brain. | Pentobarbital (30 and 60 mg/kg) and chloral hydrate (300 and 600 mg/kg) administered in anesthetic/hypnotic doses produced significant increases in acetylcholine concentration in the cerebral cortex, striatum, hippocampus and brainstem. Hypnotic/anxiolytic agents like diazepam, flurazepam (100 mg/kg each) and triazolam... | Effect of hypnotic and anxiolytic agents on regional concentration of acetylcholine in rat brain. Pentobarbital (30 and 60 mg/kg) and chloral hydrate (300 and 600 mg/kg) administered in anesthetic/hypnotic doses produced significant increases in acetylcholine concentration in the cerebral cortex, striatum, hippocampus ... | [
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PMID:24814 | Effect of estrogen and progesterone on the release of MSH in gonadectomized rats. | The concentration of MSH in serum after estrogen or progesterone injection into gonadectomized rats was measured by a biological assay. Undetectable serum MSH values were found in long-term ovariectomized (OVX) rats. After a single injection of 10 microgram estradiol benzoate (EB) serum MSH exhibited a circadiam rhythm... | Effect of estrogen and progesterone on the release of MSH in gonadectomized rats. The concentration of MSH in serum after estrogen or progesterone injection into gonadectomized rats was measured by a biological assay. Undetectable serum MSH values were found in long-term ovariectomized (OVX) rats. After a single inject... | [
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PMID:24815 | Prolactin inhibiting activity of dopamine-free subcellular fractions from rat mediobasal hypothalamus. | In order to check the hypothesis of an identity of dopamine (DA) and prolactin inhibiting activity (PIF), their subcellular distribution was studied in the mediobasal hypothalamus (MBH) and the striatum, which served as a control structure. PIF was tested both in vivo and on pituitary incubates. Fractions were also ass... | Prolactin inhibiting activity of dopamine-free subcellular fractions from rat mediobasal hypothalamus. In order to check the hypothesis of an identity of dopamine (DA) and prolactin inhibiting activity (PIF), their subcellular distribution was studied in the mediobasal hypothalamus (MBH) and the striatum, which served ... | [
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PMID:24820 | An atypical form of Sandhoff's disease. Case report and biochemical studies. | A case of Sandhoff's disease (GM2 gangliosidosis type 2) is reported because of an unusual course with later onset of symptoms, more slow progress and longer survival than those previously described. Otherwise the patient presented most of the classical symptoms of the disease with a final state of blindness, deafness ... | An atypical form of Sandhoff's disease. Case report and biochemical studies. A case of Sandhoff's disease (GM2 gangliosidosis type 2) is reported because of an unusual course with later onset of symptoms, more slow progress and longer survival than those previously described. Otherwise the patient presented most of the... | [
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PMID:24822 | Bilateral adrenalectomy: factors affecting hepatic protein synthesis following a single tube-feeding of tryptophan. | Effect of tryptophan force-feeding to well-fed adrenalectomized rats on the ability of liver cell sap and initiation factors to support protein synthesis in vitro was investigated. 5 h after tryptophan force-feeding, the capacity of liver cell sap, pH 5 enzyme and pH 5 supernatant to support protein synthesis was great... | Bilateral adrenalectomy: factors affecting hepatic protein synthesis following a single tube-feeding of tryptophan. Effect of tryptophan force-feeding to well-fed adrenalectomized rats on the ability of liver cell sap and initiation factors to support protein synthesis in vitro was investigated. 5 h after tryptophan fo... | [
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PMID:24828 | Sodium and chloride transport in the isolated human cornea. | 22Na and 36Cl fluxes in isolated human cornea preparations were measured under short-circuited conditions. At pH 7.3-PH 7.6 sodium net flux was directed from aqueous humour to tear side, chloride net flux was statistically not different from zero. Alkalinization of the bathing solution to pH 8.6 stimulated unidirection... | Sodium and chloride transport in the isolated human cornea. 22Na and 36Cl fluxes in isolated human cornea preparations were measured under short-circuited conditions. At pH 7.3-PH 7.6 sodium net flux was directed from aqueous humour to tear side, chloride net flux was statistically not different from zero. Alkalinizati... | [
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PMID:24829 | [Treatment of acute asthma. Comparison of the effectiveness of corticosteroids and of a combination of corticosteroids and an adrenergic beta-stimulant (author's transl)]. | Two comparable groups of patients hospitalised for acute asthma received an intravenous infusion for two hours, containing corticosteroids in the first group and corticosteroids combined with an adrenergic beta-stimulant in the second. Course was assessed by the hourly measurement of forced expiratory volumen in one se... | [Treatment of acute asthma. Comparison of the effectiveness of corticosteroids and of a combination of corticosteroids and an adrenergic beta-stimulant (author's transl)]. Two comparable groups of patients hospitalised for acute asthma received an intravenous infusion for two hours, containing corticosteroids in the fi... | [
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PMID:24833 | Overmethylation of DNAs by the EcoRI methylase. | EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI en... | Overmethylation of DNAs by the EcoRI methylase. EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a numb... | [
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PMID:24834 | Mammalian tRNA sulfurtransferase: properties of the enzyme in rat liver. | Transfer RNA sulfurtransferase activity was detected in 105,000 x g supernatant preparations from rat liver and several other rat tissues. Sulfur is transferred from [35S] cysteine to tRNA in a reaction which also requires ATP, Mg2+, and supernatant protein. While [35S] beta-mercaptopyruvate appeared to be a substrate ... | Mammalian tRNA sulfurtransferase: properties of the enzyme in rat liver. Transfer RNA sulfurtransferase activity was detected in 105,000 x g supernatant preparations from rat liver and several other rat tissues. Sulfur is transferred from [35S] cysteine to tRNA in a reaction which also requires ATP, Mg2+, and supernata... | [
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PMID:24835 | Choosing a drug regimen for obstructive pulmonary disease. 1. Agents to achieve bronchodilatation. | Many patients with pulmonary disease may have significant airflow obstruction. Bronchodilatation is an important aspect of treatment and can be achieved with beta-adrenergic agonists, methylxanthine derivatives, and anticholinergic agents. These can be used singly or in combination, depending on the severity of obstruc... | Choosing a drug regimen for obstructive pulmonary disease. 1. Agents to achieve bronchodilatation. Many patients with pulmonary disease may have significant airflow obstruction. Bronchodilatation is an important aspect of treatment and can be achieved with beta-adrenergic agonists, methylxanthine derivatives, and antic... | [
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PMID:24836 | Multiple endocrine adenomatosis (Type I) and familial hyperparathyroidism. | Hyperparathyroidism is the commonest presenting feature in multiple endocrine adenomatosis Type I (MEA Type I), the other manifestations may be delayed for many years or appear only in relatives. A family now diagnosed as MEA Type I, who was previously thought, in 1965, to have familial hyperparathyroidism due to chief... | Multiple endocrine adenomatosis (Type I) and familial hyperparathyroidism. Hyperparathyroidism is the commonest presenting feature in multiple endocrine adenomatosis Type I (MEA Type I), the other manifestations may be delayed for many years or appear only in relatives. A family now diagnosed as MEA Type I, who was pre... | [
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PMID:24838 | [Papain immobilization on a fibrous polymer of polyvinyl alcohol]. | The paper discusses papain immobilization on modified polyvinyl alcohol fibers by the ion fixation technique. The effect of the chemical structure of polyvinyl alcohol fibers on the basic properties of the bound enzyme has been examined. The level of activity and tolerance to pH changes of the resultant immobilized enz... | [Papain immobilization on a fibrous polymer of polyvinyl alcohol]. The paper discusses papain immobilization on modified polyvinyl alcohol fibers by the ion fixation technique. The effect of the chemical structure of polyvinyl alcohol fibers on the basic properties of the bound enzyme has been examined. The level of ac... | [
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PMID:24839 | [Immobilization of alpha-amylase on porous glass and silochrome]. | Immobilized forms of alpha-amylase from Aspergillus oryzae were prepared on the porous glass and silochrome by the glutaraldehyde method. An addition of calcium ions at a concentration of 0.05 M to the reaction mixture during immobilization stabilized the enzyme activity. pH optimum of the insoluble form of alpha-amyla... | [Immobilization of alpha-amylase on porous glass and silochrome]. Immobilized forms of alpha-amylase from Aspergillus oryzae were prepared on the porous glass and silochrome by the glutaraldehyde method. An addition of calcium ions at a concentration of 0.05 M to the reaction mixture during immobilization stabilized th... | [
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PMID:24840 | [Glutamine synthetase immobilization]. | Highly purified glutamine synthetase has been isolated from Chlorella and immobilized on BrCN-sepharose. Its residual activity was 25-35%. Immobilized glutamine synthetase showed far greater thermal stability than glutamine synthetase in solution. During immobilization pH optimum of the enzyme was shifted towards the a... | [Glutamine synthetase immobilization]. Highly purified glutamine synthetase has been isolated from Chlorella and immobilized on BrCN-sepharose. Its residual activity was 25-35%. Immobilized glutamine synthetase showed far greater thermal stability than glutamine synthetase in solution. During immobilization pH optimum ... | [
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PMID:24844 | Interaction of human chorionic gonadotropin with membrane components of rat testes. | Previous studies demonstrating that gangliosides interacted with thyrotropin and human chorionic gonadotropin (hCG) suggested that gangliosides participate in the transduction of the hormonal message across the target cell membrane. As a continuation of these investigations, we examined the effects of down-regulation o... | Interaction of human chorionic gonadotropin with membrane components of rat testes. Previous studies demonstrating that gangliosides interacted with thyrotropin and human chorionic gonadotropin (hCG) suggested that gangliosides participate in the transduction of the hormonal message across the target cell membrane. As ... | [
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PMID:24841 | [Purification and properties of the NADP-specific glutamate dehydrogenase of Candida tropicalis feed yeasts]. | From the cell-free extract of fodder yeast Candida tropicalis NADP-specific glutamate dehydrogenase was isolated and partially purified (75-fold) by means of fractional precipitation by ammonium sulphate and ion-exchange chromatography on DEAE-cellulose. The preparation was investigated with the aid of polyacrylamide g... | [Purification and properties of the NADP-specific glutamate dehydrogenase of Candida tropicalis feed yeasts]. From the cell-free extract of fodder yeast Candida tropicalis NADP-specific glutamate dehydrogenase was isolated and partially purified (75-fold) by means of fractional precipitation by ammonium sulphate and io... | [
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PMID:24845 | Role of anions in parathyroid hormone release from dispersed bovine parathyroid cells. | It is known that permeant anions are required for the release of epinephrine from isolated chromaffin granules and of serotonin from intact platelets. We have now investigated the role of anions in the release of a polypeptide hormone, parathyroid hormone, from dispersed bovine parathyroid cells. The release is inhibit... | Role of anions in parathyroid hormone release from dispersed bovine parathyroid cells. It is known that permeant anions are required for the release of epinephrine from isolated chromaffin granules and of serotonin from intact platelets. We have now investigated the role of anions in the release of a polypeptide hormon... | [
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PMID:24846 | Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance. | High-resolution phosphorus-31 nuclear magnetic resonance spectra were observed for cell lines from mammalian sources: lymphoid cells, Friend erythroleukemia cells, and HeLa cells. Some phosphate metabolites were identified and their concentrations were found to vary among the different cell lines. ATP/ADP ratios could ... | Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance. High-resolution phosphorus-31 nuclear magnetic resonance spectra were observed for cell lines from mammalian sources: lymphoid cells, Friend erythroleukemia cells, and HeLa cells. Some p... | [
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PMID:24847 | Inhibition of growth and guanylate cyclase activity of an undifferentiated prostate adenocarcinoma by an extract of the balsam pear (Momordica charantia abbreviata). | We have recently described the presence of a guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] inhibitor (GCI) in an aqueous extract of the balsam pear (Momordica charantia abbreviata). Because the guanylate cyclase-cyclic GMP system is though to be involved in cell growth, DNA and RNA synthesis, and ... | Inhibition of growth and guanylate cyclase activity of an undifferentiated prostate adenocarcinoma by an extract of the balsam pear (Momordica charantia abbreviata). We have recently described the presence of a guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] inhibitor (GCI) in an aqueous extract of ... | [
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PMID:24842 | [Microbiological transformation of carbohydrates. Glucose isomerization into fructose by washed cells of Streptomyces sp. strain 29]. | Glucose-fructose transformation by washed and packed cells of Streptomyces sp. 29 was studied. The cells grown on the nutrient medium containing xylose and Co and Mg salts were capable to perform glucose-fructose isomerization. The cellular activity depended in a great degree on the temperature, pH and initial glucose ... | [Microbiological transformation of carbohydrates. Glucose isomerization into fructose by washed cells of Streptomyces sp. strain 29]. Glucose-fructose transformation by washed and packed cells of Streptomyces sp. 29 was studied. The cells grown on the nutrient medium containing xylose and Co and Mg salts were capable t... | [
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PMID:24843 | [Hypoglycemic effect of nicotinamide in rats with alloxan diabetes]. | Experimentally-induced alloxan diabetes was characterized in rats by a marked increase in the blood glucose level and by a number of disturbances in the concentration of metabolites and the activity of the enzymes of carbohydrate metabolism in the liver. Stimulation of gluconeogenesis in diabetes was judged by reductio... | [Hypoglycemic effect of nicotinamide in rats with alloxan diabetes]. Experimentally-induced alloxan diabetes was characterized in rats by a marked increase in the blood glucose level and by a number of disturbances in the concentration of metabolites and the activity of the enzymes of carbohydrate metabolism in the liv... | [
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PMID:24855 | Fluorimetric determination of the active form of tetracycline, chloretetracycline and oxytetracycline in partially decomposed solutions. | The content of tetracycline (1), chlortetracycline (2) and oxytetracycline (3) has been determined by use of the fluorimetric method in partially decomposed acqueous solutions of different pH values. The procedure consisted in the extraction of fluorescent calcium and 5.5-diethyl-barbituric acid complexes of 1 and 3 (w... | Fluorimetric determination of the active form of tetracycline, chloretetracycline and oxytetracycline in partially decomposed solutions. The content of tetracycline (1), chlortetracycline (2) and oxytetracycline (3) has been determined by use of the fluorimetric method in partially decomposed acqueous solutions of diff... | [
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PMID:24859 | The effect of stress on the activity of hepatic tryptophan pyrrolase, of tyrosine aminotransferase in various organs and on the level of tryptophan in the liver and plasma of rats. | In rats subjected to 400 revolutions in Noble-Collip drums, hepatic tryptophan pyrrolase activity increases and plasma tryptophan level decreases. After bilateral adrenalectomy, the alterations of plasma tryptophan are even more pronounced and liver tryptophan increases in contrast to tryptophan pyrrolase activity whic... | The effect of stress on the activity of hepatic tryptophan pyrrolase, of tyrosine aminotransferase in various organs and on the level of tryptophan in the liver and plasma of rats. In rats subjected to 400 revolutions in Noble-Collip drums, hepatic tryptophan pyrrolase activity increases and plasma tryptophan level dec... | [
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PMID:24860 | [Behavioral rehabilitation of nonspecific symptoms occuring after craniocerbral trauma (author's transl)]. | The purpose of this study was to develop a behavioral rehabilitation program for patients with long-term nonspecific symptoms following craniocerebral trauma. In 47 patients we analyzed symptoms typical of "illness behavior" (e.g. complaining tendency, depression, irritability, psychosomatic pain, problems in job perfo... | [Behavioral rehabilitation of nonspecific symptoms occuring after craniocerbral trauma (author's transl)]. The purpose of this study was to develop a behavioral rehabilitation program for patients with long-term nonspecific symptoms following craniocerebral trauma. In 47 patients we analyzed symptoms typical of "illnes... | [
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PMID:24866 | Oxygen affinity and 2,3-diphosphoglycerate in blood of Australian marsupials of differing body size. | Oxygen carriage and 2,3-diphosphoglycerate (2,3-DPG) levels have been measured in the blood of seven species of Australian marsupials ranging in size from 35 to 0.03 kg. They were Red and Grey Kangaroos, Wallaroo, Tammar Wallaby, Brush-tailed possum, Potoroo, and Brown Marsupial Mouse. Oxygen affinity decreased with de... | Oxygen affinity and 2,3-diphosphoglycerate in blood of Australian marsupials of differing body size. Oxygen carriage and 2,3-diphosphoglycerate (2,3-DPG) levels have been measured in the blood of seven species of Australian marsupials ranging in size from 35 to 0.03 kg. They were Red and Grey Kangaroos, Wallaroo, Tamma... | [
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PMID:24867 | Metabolic, respiratory and haematological adjustments of the little pocket mouse to circadian torpor cycles. | Metabolic, respiratory and haematological parameters were investigated for the Little Pocket mouse during circadian torpor cycles. The rate of O2 consumption decreased from 7.04 to 0.05 ml O2.g-1.hr-1, with a corresponding decrease in respiratory minute volume from 49.4 to 0.9 ml.min-1 during torpor at an ambient tempe... | Metabolic, respiratory and haematological adjustments of the little pocket mouse to circadian torpor cycles. Metabolic, respiratory and haematological parameters were investigated for the Little Pocket mouse during circadian torpor cycles. The rate of O2 consumption decreased from 7.04 to 0.05 ml O2.g-1.hr-1, with a co... | [
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PMID:24868 | A comparative study of the respiratory properties of bird blood. | Oxygen affinity and Bohr effect have been measured in the blood of four bird species (chicken, pigeon, Japanese quail and sparrow). Under physiological conditions (41 C, pH 7.5, PCO2 approximately 35 Torr) the oxygen half saturation pressure P50 are 50 Torr for the chickens, 38 Torr for the pigeon, 43 Torr for the Japa... | A comparative study of the respiratory properties of bird blood. Oxygen affinity and Bohr effect have been measured in the blood of four bird species (chicken, pigeon, Japanese quail and sparrow). Under physiological conditions (41 C, pH 7.5, PCO2 approximately 35 Torr) the oxygen half saturation pressure P50 are 50 To... | [
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PMID:24869 | Regulation of arterial PCO2 during inhalation of CO2 in chickens. | Since CO2-sensitive intrapulmonary chemoreceptors have been shown to exist in the avian lung, we conducted a series of experiments to determine if birds could regulate PaCO2 when confronted with an inspired CO2 load. Chickens were anesthetized with either pentobarbital (30 mg.kg-1) or phenobarbital (160 mg.kg-1). The P... | Regulation of arterial PCO2 during inhalation of CO2 in chickens. Since CO2-sensitive intrapulmonary chemoreceptors have been shown to exist in the avian lung, we conducted a series of experiments to determine if birds could regulate PaCO2 when confronted with an inspired CO2 load. Chickens were anesthetized with eithe... | [
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PMID:24870 | The transduction properties of tracheal stretch receptors in vitro. | Extrathoracic tracheal stretch receptors and tracheal tension were recorded in vitro when the trachea was stretched. The receptors responded to an increase in tension produced by displacement in the longitudinal or transverse axis of the trachea. They were sensitive to the rate of change of tension as well as the tensi... | The transduction properties of tracheal stretch receptors in vitro. Extrathoracic tracheal stretch receptors and tracheal tension were recorded in vitro when the trachea was stretched. The receptors responded to an increase in tension produced by displacement in the longitudinal or transverse axis of the trachea. They ... | [
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PMID:24889 | Calcium-stimulated ATPase activity in homogenates of the secretory enamel organ in the rat. | The activity of calcium-stimulated ATPase (E.C. 3.6.1.3) in homogenates of the secretory enamel organ of rat incisors was studied biochemically. ATP hydrolysis was estimated from the amount of inorganic phosphate liberated. An analysis of the total degradation of ATP was initially performed to ensure that the enzyme as... | Calcium-stimulated ATPase activity in homogenates of the secretory enamel organ in the rat. The activity of calcium-stimulated ATPase (E.C. 3.6.1.3) in homogenates of the secretory enamel organ of rat incisors was studied biochemically. ATP hydrolysis was estimated from the amount of inorganic phosphate liberated. An a... | [
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PMID:24890 | Demonstration of a K+-stimulated and ouabain-sensitive p-nitrophenyl phosphatase activity in enamel-and dentin-forming tissues in the rat. | p-Nitrophenyl phosphate hydrolysis was studied at neutral pH with tissue preparations of the rat secretory and maturation enamel organs and dental pulp. By introduction of inhibitors to nonspecific alkaline phosphatase activity and stimulants to the K+-stimulated and ouabain-sensitive p-nitrophenyl phosphatase activity... | Demonstration of a K+-stimulated and ouabain-sensitive p-nitrophenyl phosphatase activity in enamel-and dentin-forming tissues in the rat. p-Nitrophenyl phosphate hydrolysis was studied at neutral pH with tissue preparations of the rat secretory and maturation enamel organs and dental pulp. By introduction of inhibitor... | [
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PMID:24894 | Bicarbonate ion transport: a mechanism for the acidification of urine in the turtle. | The uricotelic turtle Pseudemys scripta acidifies the urine to a pH as low as 4 in the urinary bladder. Data in this report show that the mechanism of acidification in this bladder is the transport of bicarbonate ion from lumen to serosa, and that the temperature to which the turtles are adapted prior to the in vitro e... | Bicarbonate ion transport: a mechanism for the acidification of urine in the turtle. The uricotelic turtle Pseudemys scripta acidifies the urine to a pH as low as 4 in the urinary bladder. Data in this report show that the mechanism of acidification in this bladder is the transport of bicarbonate ion from lumen to sero... | [
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PMID:24895 | Oral cocaine: plasma concentrations and central effects. | Cocaine (2.0 milligrams per kilogram) given by the oral route is at least as effective as the same dose given intranasally. Cocaine is not detected in the plasma until 30 minutes after oral administration, but peak plasma concentrations are similar after both routes. The subjective "highs" in man are greater after oral... | Oral cocaine: plasma concentrations and central effects. Cocaine (2.0 milligrams per kilogram) given by the oral route is at least as effective as the same dose given intranasally. Cocaine is not detected in the plasma until 30 minutes after oral administration, but peak plasma concentrations are similar after both rou... | [
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PMID:24896 | MEN type IIa syndrome: dilemmas in modern management. | With the use of newer techniques, the multiple endocrine neoplasm II-A syndrome can now be diagnosed early in its evolution. This article discusses the clinical course of the syndrome, the diagnostic modalities employed, and the therapeutic interventions required. An approach to the individual patient with this syndrom... | MEN type IIa syndrome: dilemmas in modern management. With the use of newer techniques, the multiple endocrine neoplasm II-A syndrome can now be diagnosed early in its evolution. This article discusses the clinical course of the syndrome, the diagnostic modalities employed, and the therapeutic interventions required. A... | [
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PMID:24898 | Muscle perfusion and the healing of below knee amputations. | Muscle pH has been used to assess the perfusion of the muscles at the site of below knee amputation in 20 patients having ischemia at the periphery. The results show that a muscle pH above 7.0 did not correlate with the outcome of amputation but that, when the pH was below 7.00, healing was never achieved. | Muscle perfusion and the healing of below knee amputations. Muscle pH has been used to assess the perfusion of the muscles at the site of below knee amputation in 20 patients having ischemia at the periphery. The results show that a muscle pH above 7.0 did not correlate with the outcome of amputation but that, when the... | [
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PMID:24899 | The role of fibrin formation in the pathogenesis of bacteremic shock in the primate. | In this study, an attempt was made to elucidate further the role of intravascular fibrin formation in the pathogenesis of sepsis in the primate. It was found that injected live Escherichia coli caused death in primates within four to 11 hours as a result of microcirculatory failure and acidosis. Pretreatment with Arvin... | The role of fibrin formation in the pathogenesis of bacteremic shock in the primate. In this study, an attempt was made to elucidate further the role of intravascular fibrin formation in the pathogenesis of sepsis in the primate. It was found that injected live Escherichia coli caused death in primates within four to 1... | [
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PMID:24901 | Training and use of surgeon's assistants. | "Physician extenders" of various types have been produced in the past few years. The University of Alabama in Birmingham has trained surgeon's assistants over the past 9 years, graduating 53 individuals after a 2-year course of didactic, laboratory, and clinical instruction. The graduates have performed satisfactorily,... | Training and use of surgeon's assistants. "Physician extenders" of various types have been produced in the past few years. The University of Alabama in Birmingham has trained surgeon's assistants over the past 9 years, graduating 53 individuals after a 2-year course of didactic, laboratory, and clinical instruction. Th... | [
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PMID:24902 | Droperidol, its alpha-adrenergic blocking action on the aortic strip and inhibitory action on norepinephrine uptake of the adrenergic terminal of the left atrial strip of rabbit. | In aortic strips, the dose-response curves for phenylephrine were obtained before and after addition of droperidol. Droperidol caused a parallel shift of the curves dose-dependently toward the right side and its grade of the shift was greater than caused by phentolamine at the same concentration. In the left atrial str... | Droperidol, its alpha-adrenergic blocking action on the aortic strip and inhibitory action on norepinephrine uptake of the adrenergic terminal of the left atrial strip of rabbit. In aortic strips, the dose-response curves for phenylephrine were obtained before and after addition of droperidol. Droperidol caused a paral... | [
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PMID:24911 | The mechanism of urinary continence after prostatectomy. | An anatomical and neuropharmacological study of the urethra was performed in 19 patients after prostatetectomy to investigate the mechanism of continence. Anatomically, the urethral segment responsible for the postprostatectomy continence was located distal to the open prostatic fossa in the external sphincter which is... | The mechanism of urinary continence after prostatectomy. An anatomical and neuropharmacological study of the urethra was performed in 19 patients after prostatetectomy to investigate the mechanism of continence. Anatomically, the urethral segment responsible for the postprostatectomy continence was located distal to th... | [
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PMID:24912 | Pathology of germ cell tumors of the testis. | Testicular germ cell tumors form a complex group of interrelated entities. Problems of nomenclature remain, but there does appear to be a close agreement between the major classifications in use. The identification of clinically useful markers for certain germ cell testicular tumors has provided a major impetus in this... | Pathology of germ cell tumors of the testis. Testicular germ cell tumors form a complex group of interrelated entities. Problems of nomenclature remain, but there does appear to be a close agreement between the major classifications in use. The identification of clinically useful markers for certain germ cell testicula... | [
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PMID:24915 | Cytogenic studies of cryptorchid testes. | Twenty-nine patients with unilateral or bilateral cryptochism underwent biopsies of the testis at the time of orchiopexy. Karyotype evaluation of fibroblasts obtained from tissue cultures of the biopsy specimen was completed counting a minimum of twenty-five cells. In 1 patient with multiple anomalies a 46,XYDq+ karyot... | Cytogenic studies of cryptorchid testes. Twenty-nine patients with unilateral or bilateral cryptochism underwent biopsies of the testis at the time of orchiopexy. Karyotype evaluation of fibroblasts obtained from tissue cultures of the biopsy specimen was completed counting a minimum of twenty-five cells. In 1 patient ... | [
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PMID:24916 | [Evaluation of metabolic profile tests using a computer]. | A programme has been developed for the evaluation of metabolism profile tests in dairy cows by means of the TESLA-200 computer in the FORTRAN language. The computer evaluates and plots the metabolism profile from input data punched on tape in the IBM code by the Consul-253 electronic organization automatic device. The ... | [Evaluation of metabolic profile tests using a computer]. A programme has been developed for the evaluation of metabolism profile tests in dairy cows by means of the TESLA-200 computer in the FORTRAN language. The computer evaluates and plots the metabolism profile from input data punched on tape in the IBM code by the... | [
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PMID:24917 | [Intensity of acid-base reactions of the blood and cerebrospinal fluid in sheep after intraluminal stress induced by urea and acetic acid]. | Acid-base indices of the blood and cerebro-spinal fluid were studied on Astrup apparatus (BMS 2) in an experiment with twelve, two- to three-year-old Merino sheep, being in the first half of the gravidity period, under clinical conditions after the administration of 0.8 g urea per 1 kg live weight followed by, in half ... | [Intensity of acid-base reactions of the blood and cerebrospinal fluid in sheep after intraluminal stress induced by urea and acetic acid]. Acid-base indices of the blood and cerebro-spinal fluid were studied on Astrup apparatus (BMS 2) in an experiment with twelve, two- to three-year-old Merino sheep, being in the fir... | [
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PMID:24918 | Polypoid nonchromaffin paraganglioma of the duodenum. | A polypoid tumor was surgically removed from the second part of the duodenum of a 56-year-old male. The main body consisted of large epithelioid cells arranged in an adenoma like pattern of strands and nests. These cells were argyrophil and had marked nonspecific esterase activity. Unmyelinated nerves with proliferated... | Polypoid nonchromaffin paraganglioma of the duodenum. A polypoid tumor was surgically removed from the second part of the duodenum of a 56-year-old male. The main body consisted of large epithelioid cells arranged in an adenoma like pattern of strands and nests. These cells were argyrophil and had marked nonspecific es... | [
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PMID:24919 | [Artificial arterial hypotension during surgical treatment of intracranial aneurysms]. | In studying the content of lactic and pyruvic acids in the cerebrospinal fluid of 31 patients during operation for intracranial aneurysms under halothane anesthesia, the authors established that the performance of these operations under protection of deep (40 mm Hg) and prolonged (up to 2 hrs 25 min) artificial arteria... | [Artificial arterial hypotension during surgical treatment of intracranial aneurysms]. In studying the content of lactic and pyruvic acids in the cerebrospinal fluid of 31 patients during operation for intracranial aneurysms under halothane anesthesia, the authors established that the performance of these operations un... | [
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PMID:24923 | Dehydrogenation of indanol by rabbit liver 3-hydroxyhexobarbital dehydrogenase. | 1. Among the several enzyme activities in rabbit liver cytosol able to dehydrogenate 1-indanol, only the main activity was not separable from 3-hydroxyhexobarbital dehydrogenase during purification including polyacrylamide gel disc electrophoresis. 2. Results of mixed substrate method indicated that the same enzyme cat... | Dehydrogenation of indanol by rabbit liver 3-hydroxyhexobarbital dehydrogenase. 1. Among the several enzyme activities in rabbit liver cytosol able to dehydrogenate 1-indanol, only the main activity was not separable from 3-hydroxyhexobarbital dehydrogenase during purification including polyacrylamide gel disc electrop... | [
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PMID:24924 | [Anabolic and catabolic enzymes of urea metabolism in a carbohydrate-utilizing strain of Candida guilliermondii]. | The yeast "H" of the genus Candida guilliermondii can grow on hydrocarbons as the only source for carbon. Urea can serve as a nitrogen source for this yeast which lacks detectable urease activity. During urea metabolism ammonia has never been accumulated in the culture medium. However, transferring the yeast from compl... | [Anabolic and catabolic enzymes of urea metabolism in a carbohydrate-utilizing strain of Candida guilliermondii]. The yeast "H" of the genus Candida guilliermondii can grow on hydrocarbons as the only source for carbon. Urea can serve as a nitrogen source for this yeast which lacks detectable urease activity. During ur... | [
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PMID:24925 | [On molecular biology of aging. 11th communication. Enzyme adaptation, age (author's transl)]. | Age-dependent modifications of enzyme adaptations in response to a variety of environmental alterations are a biochemical expression of biological ageing. In this paper age-dependent changes of the adaptation of a number of enzymes are described. Experiments from our laboratory indicate that pyruvate kinase is more res... | [On molecular biology of aging. 11th communication. Enzyme adaptation, age (author's transl)]. Age-dependent modifications of enzyme adaptations in response to a variety of environmental alterations are a biochemical expression of biological ageing. In this paper age-dependent changes of the adaptation of a number of e... | [
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PMID:24961 | Effect of lime-pelleting on nodulation and yield of soybean grown in acid soil. | An experiment for studying the effect of lime-pelleting of inoculated seed of soybean [Glycine max. (L.) Merr.] on nodulation, growth, and grain yield was undertaken in red sandy loam of Bangalore, having a pH of 4.0. The results indicated that inoculation alone increased significantly nodulation, plant dry weight, and... | Effect of lime-pelleting on nodulation and yield of soybean grown in acid soil. An experiment for studying the effect of lime-pelleting of inoculated seed of soybean [Glycine max. (L.) Merr.] on nodulation, growth, and grain yield was undertaken in red sandy loam of Bangalore, having a pH of 4.0. The results indicated ... | [
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PMID:24962 | [Application of a TRICIN-buffered modification of the CMRL-1969 medium in cell and virus cultivation (author's transl)]. | CMRL-1969 medium (Healy et al., 1971) was modified by using 0.02 molar N-[Tris-(hydroxymethyl)-methyl]-glycin (= TRICIN) instead of bicarbonate as the buffer substance. Several permanent cell lines and primary cell cultures did not show growth differences in the two medium variants. Like other non volatile buffers TRIC... | [Application of a TRICIN-buffered modification of the CMRL-1969 medium in cell and virus cultivation (author's transl)]. CMRL-1969 medium (Healy et al., 1971) was modified by using 0.02 molar N-[Tris-(hydroxymethyl)-methyl]-glycin (= TRICIN) instead of bicarbonate as the buffer substance. Several permanent cell lines a... | [
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PMID:24963 | Clotting and fibrinolytic activities of Bacteroides melaninogenicus. | 106 strains of Bacteroides melaninogenicus ss. asaccharolyticus, intermedius and melaninogenicus were tested for production of extracellular blood clotting and fibrinolytic factors. 78.3% of tested strains caused clotting of rabbit plasma and 79.2% activated human plasminogen. Strains producing the clotting or fibrinol... | Clotting and fibrinolytic activities of Bacteroides melaninogenicus. 106 strains of Bacteroides melaninogenicus ss. asaccharolyticus, intermedius and melaninogenicus were tested for production of extracellular blood clotting and fibrinolytic factors. 78.3% of tested strains caused clotting of rabbit plasma and 79.2% ac... | [
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PMID:24964 | Studies on antituberculotic action of some phenothiazine derivatives in vitro. | Five phenothiazine derivatives (chlorpromazine, levomepromazine, diethazine, promethazine and chlorpromazine) sulphoxyde were tested for antimycobacterial activity. The growth of Mycobacterium tuberculosis, M. bovis and M. butyricum was inhibited by chlorpromazine practically at identical concentrations. The minimum in... | Studies on antituberculotic action of some phenothiazine derivatives in vitro. Five phenothiazine derivatives (chlorpromazine, levomepromazine, diethazine, promethazine and chlorpromazine) sulphoxyde were tested for antimycobacterial activity. The growth of Mycobacterium tuberculosis, M. bovis and M. butyricum was inhi... | [
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PMID:24965 | The nature of clotting and fibrinolytic activities of Bacteroids melaninogenicus. | From the supernatant of B. melaninogenicus ss. asaccharolyticus culture, a protein fraction was isolated by ethanol precipitation. The fraction was tested for the presence of clotting and fibrinolytic activities by application of quantitative techniques and specific substrates for measurement of prothrombin and plasmin... | The nature of clotting and fibrinolytic activities of Bacteroids melaninogenicus. From the supernatant of B. melaninogenicus ss. asaccharolyticus culture, a protein fraction was isolated by ethanol precipitation. The fraction was tested for the presence of clotting and fibrinolytic activities by application of quantita... | [
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PMID:24966 | [Microbial growth on an expansion joint of a reservoir for drinkingwater (author's transl)]. | In a clear water reservoir built in ready construction after a working-period of five months quite a lot of slime could be found on the expansion joint filled with tightening compound on the base of Thiokol. The covering slime could be removed easily with a rubber-screen. The microbiological analysis showed an extremel... | [Microbial growth on an expansion joint of a reservoir for drinkingwater (author's transl)]. In a clear water reservoir built in ready construction after a working-period of five months quite a lot of slime could be found on the expansion joint filled with tightening compound on the base of Thiokol. The covering slime ... | [
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PMID:24967 | [On the determination of chlorine with a polarographic probe. 2nd communication: experimental arrangement and results under application (author's transl)]. | The examination of the physical properties of the instrument showed several possibilities of meter-error mainly due to constructive disadvantages. After having found this a special experimental arrangement was worked out to exclude errors. Then good linear correlations of the meter-reading and the analytical DPD-Method... | [On the determination of chlorine with a polarographic probe. 2nd communication: experimental arrangement and results under application (author's transl)]. The examination of the physical properties of the instrument showed several possibilities of meter-error mainly due to constructive disadvantages. After having foun... | [
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PMID:24968 | [Acid-base equilibrium in patients with tick-borne encephalitis and similar diseases]. | The study is concerned with the acid-base balance in 92 patients with tick-borne encephalitis, tick-borne fever and meningitis of an obscure etiology. In many cases there was a shift in the direction of a metabolic acidosis, which was most vividly expressed in the meningitic form of tick-borne encephalitis. Some of the... | [Acid-base equilibrium in patients with tick-borne encephalitis and similar diseases]. The study is concerned with the acid-base balance in 92 patients with tick-borne encephalitis, tick-borne fever and meningitis of an obscure etiology. In many cases there was a shift in the direction of a metabolic acidosis, which wa... | [
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PMID:24969 | [Use of neuroleptics for the treatment of chronic alcoholism (psychopathology of the attraction to alcohol)]. | A psychopathological analysis of alcoholic drives in patients with alcoholism demonstrates that along with well-known negative symptoms of an alcoholic mental defect there is a productive psychopathological symptom in the clinical picture as well. Having this view, the author in 148 cases of alcoholism applied etoperas... | [Use of neuroleptics for the treatment of chronic alcoholism (psychopathology of the attraction to alcohol)]. A psychopathological analysis of alcoholic drives in patients with alcoholism demonstrates that along with well-known negative symptoms of an alcoholic mental defect there is a productive psychopathological sym... | [
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PMID:24970 | Effect of extracellular pH and inhibitors on the gluconeogenesis and ammoniagenesis relationship in rat kidney cortex slices. | 1. Gluconeogenesis from glutamine, fumarate, pyruvate, glutamine plus fumarate, and glutamine plus pyruvate, was generally higher at pH 7.1 than at pH 7.4 and 7.7, whereas ammoniagenesis did not depend on the pH of the medium. 2. The intermediates of the Krebs cycle decreased ammonia formation from glutamine, raising a... | Effect of extracellular pH and inhibitors on the gluconeogenesis and ammoniagenesis relationship in rat kidney cortex slices. 1. Gluconeogenesis from glutamine, fumarate, pyruvate, glutamine plus fumarate, and glutamine plus pyruvate, was generally higher at pH 7.1 than at pH 7.4 and 7.7, whereas ammoniagenesis did not... | [
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PMID:24978 | Neuroleptic blockade of the effect of various neurotransmitter substances. | The antagonistic effect of neuroleptics to acetylcholine, histamine, 5-HT, and noradrenaline was examined in various in vivo and in vitro models. Piflutixol, a new potent thioxanthene neuroleptic, markedly antagonizes the effect of dopamine, noradrenaline, 5-HT and to some extent histamine, whereas the affinity for mus... | Neuroleptic blockade of the effect of various neurotransmitter substances. The antagonistic effect of neuroleptics to acetylcholine, histamine, 5-HT, and noradrenaline was examined in various in vivo and in vitro models. Piflutixol, a new potent thioxanthene neuroleptic, markedly antagonizes the effect of dopamine, nor... | [
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PMID:24980 | Efficacy and side effects of flurazepam, fosazepam, and nitrazepam as sleeping aids in psychogeriatric patients. | Efficacy and side effects of flurazepam 15 mg, fosazepam 60 mg, and nitrazepam 5 mg were studied in 17 psychogeriatric patients. The drugs were equipotent in maintaining sleep but nitrazepam had more side effects than the other hypnotics, and it induced a rebound insomnia after withdrawal. All hypnotics lost some of th... | Efficacy and side effects of flurazepam, fosazepam, and nitrazepam as sleeping aids in psychogeriatric patients. Efficacy and side effects of flurazepam 15 mg, fosazepam 60 mg, and nitrazepam 5 mg were studied in 17 psychogeriatric patients. The drugs were equipotent in maintaining sleep but nitrazepam had more side ef... | [
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PMID:24981 | Three years' maintenance neuroleptic treatment in schizophrenia--before and beyond. | A group of patients with schizophrenia, initially 67 patients, was studied over a period of 3 years. After three years 36 out of 67 patients were still on the same depot neuroleptic. The main aim was to describe and compare maintenance neuroleptic therapy using two depot neuroleptics, fluphenazine decanoate and pipotia... | Three years' maintenance neuroleptic treatment in schizophrenia--before and beyond. A group of patients with schizophrenia, initially 67 patients, was studied over a period of 3 years. After three years 36 out of 67 patients were still on the same depot neuroleptic. The main aim was to describe and compare maintenance ... | [
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PMID:24982 | How schizophrenic patients change during 3 years' treatment with depot neuroleptics. | A group of patients, initially 67 individuals, with chronic schizophrenia were studied on repeated occasions during 1 year and followed up after 3 years. The patients were given depot neuroleptics, either fluphenazine decanoate or pipotiazine palmitate, at intervals of 1 month. The symptom scores from three rating scal... | How schizophrenic patients change during 3 years' treatment with depot neuroleptics. A group of patients, initially 67 individuals, with chronic schizophrenia were studied on repeated occasions during 1 year and followed up after 3 years. The patients were given depot neuroleptics, either fluphenazine decanoate or pipo... | [
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PMID:24985 | Auricular tyrosine hydroxylase and dopamine-beta-hydroxylase activities and noradrenaline content in ischaemic heart disease. | Assessments were made of the tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) activities as well as the noradrenaline (NA) content of samples excised from right auricular tissue during cardiac surgery on a total of 55 patients with ischaemic heart disease (IHD), valvular heart disease (VHD), uncomplicated ... | Auricular tyrosine hydroxylase and dopamine-beta-hydroxylase activities and noradrenaline content in ischaemic heart disease. Assessments were made of the tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) activities as well as the noradrenaline (NA) content of samples excised from right auricular tissue dur... | [
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PMID:24989 | Cyclic nucleotide metabolism in solid tumor tissues. | The examination of the regulation of the system of 3'-5' cyclic nucleotide monophosphates has only begun in cancer tissues. In human cancers, these studies are notably non-existent. However, in animal cancers, especially the Morris hepatomas, enough data has been gathered that, while risky, certain trends seem to begin... | Cyclic nucleotide metabolism in solid tumor tissues. The examination of the regulation of the system of 3'-5' cyclic nucleotide monophosphates has only begun in cancer tissues. In human cancers, these studies are notably non-existent. However, in animal cancers, especially the Morris hepatomas, enough data has been gat... | [
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PMID:24994 | Quantitative effects of salycylhydroxamic acid and glycerol on Trypanosoma brucei glycolysis in vitro and in vivo. | During anaerobic glycolysis in vitro in the presence of salicylhydroxamic acid, Trypanosoma brucei brucei converts glucose to equimolar amounts of glycerol and pyruvate as end products. Glycerol, whether generated endogenously and pyruvate as end products. Glycerol, whether generated endogenously or added exogenously, ... | Quantitative effects of salycylhydroxamic acid and glycerol on Trypanosoma brucei glycolysis in vitro and in vivo. During anaerobic glycolysis in vitro in the presence of salicylhydroxamic acid, Trypanosoma brucei brucei converts glucose to equimolar amounts of glycerol and pyruvate as end products. Glycerol, whether g... | [
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PMID:24995 | Effect of beta-lapachone on hydrogen peroxide production in Trypanosoma cruzi. | beta-Lapachone, an antimicrobial agent, markedly increase the generation of H2O2 in intact Trypanosoma cruzi epimastigotes (Y strain). Increase in H2O2 was determined by the horseradish peroxidase-H2O2 Compound II formation as well as by a cytochemical technique. | Effect of beta-lapachone on hydrogen peroxide production in Trypanosoma cruzi. beta-Lapachone, an antimicrobial agent, markedly increase the generation of H2O2 in intact Trypanosoma cruzi epimastigotes (Y strain). Increase in H2O2 was determined by the horseradish peroxidase-H2O2 Compound II formation as well as by a c... | [
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PMID:24996 | [Data on the life cycle of Ribeiroia marini guadeloupensis n. ssp., a trematode sterilizing Biomphalaria glabrata in Guadeloupe]. | Data on the life cycle of Ribeiroia marini guadeloupensis n.ssp., Trematode sterilizing Biomphalaria glabrata in Guadeloupe. Maintenance of the life cycle with a view to an eventual control of the populations of Molluscs. In Guadeloupe (French West Indies), the definitive hosts of Ribeiroia marini guadeloupensis n.ssp.... | [Data on the life cycle of Ribeiroia marini guadeloupensis n. ssp., a trematode sterilizing Biomphalaria glabrata in Guadeloupe]. Data on the life cycle of Ribeiroia marini guadeloupensis n.ssp., Trematode sterilizing Biomphalaria glabrata in Guadeloupe. Maintenance of the life cycle with a view to an eventual control ... | [
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PMID:24998 | Alteration of amicrofilaremia in Dipetalonema viteae infected hamsters with immunosuppressive drugs. | Dipetalonema viteae-infected hamsters with amicrofilaremic infections were subjected to immunosuppressive therapy. Methyl prednisolone acetate caused the most severe recrudescence of microfilariae while cyclophosphamide caused a low level, transient microfilaremia. Saline injected control hamsters remained amicrofilare... | Alteration of amicrofilaremia in Dipetalonema viteae infected hamsters with immunosuppressive drugs. Dipetalonema viteae-infected hamsters with amicrofilaremic infections were subjected to immunosuppressive therapy. Methyl prednisolone acetate caused the most severe recrudescence of microfilariae while cyclophosphamide... | [
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PMID:24999 | Serodiagnosis of human cysticercosis by microplate enzyme-linked immunospecific assay (ELISA). | The micro-ELISA described in this report is simple and can give objective results for the serodiagnosis of human cysticercosis. The visual assessment of results was satisfactory. The ELISA results showed a remarkably good correlation with IHA. It gave good reproudcibility. Cross-reactions were eliminated by using whole... | Serodiagnosis of human cysticercosis by microplate enzyme-linked immunospecific assay (ELISA). The micro-ELISA described in this report is simple and can give objective results for the serodiagnosis of human cysticercosis. The visual assessment of results was satisfactory. The ELISA results showed a remarkably good cor... | [
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PMID:25000 | Responses of Anopheles minimus to DDT residual spraying in a cleared forested foothill area in central Thailand. | Anopheles balabacensis balabacensis and Anopheles minimus are the main malaria vectors in Thailand. In a cleared forested foothill area in the central part of the country A. minimus was the most prevalent anopheline species found, only 6 specimens of A. b. balabacensis being collected over a 3-year period. Cattle were ... | Responses of Anopheles minimus to DDT residual spraying in a cleared forested foothill area in central Thailand. Anopheles balabacensis balabacensis and Anopheles minimus are the main malaria vectors in Thailand. In a cleared forested foothill area in the central part of the country A. minimus was the most prevalent an... | [
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PMID:25001 | [Studies on an isolated tribe in the central highlands of Irian-Jaya (Indonesian New Guinea) within the field of tropical medicine. I. Clinical studies]. | Reports on medical research examinations in the field of tropical medicine of 130 indigenous, who were examined in the central Highland of West-Irian during an interdisciplinare research project. The inhabitants of this markedly isolated area are pygmies of a strong, well proportioned build of the body and in a relativ... | [Studies on an isolated tribe in the central highlands of Irian-Jaya (Indonesian New Guinea) within the field of tropical medicine. I. Clinical studies]. Reports on medical research examinations in the field of tropical medicine of 130 indigenous, who were examined in the central Highland of West-Irian during an interd... | [
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PMID:25002 | Radioimmunoassay of influenza A virus haemagglutinin. I. Preparation and properties of radioactive 125I-labelled bromelain-released haemagglutinin. | Haemagglutinin released from influenza A virus recombinant MRC11 [antigenically identical to the strain A/Port Chalmers/1/73 (H3N2)] by bromelain treatment and purified by rate zonal centrifugation (further on B-HA) was examined for eventual contamination by neuraminidase. According to specific enzymatic activities cor... | Radioimmunoassay of influenza A virus haemagglutinin. I. Preparation and properties of radioactive 125I-labelled bromelain-released haemagglutinin. Haemagglutinin released from influenza A virus recombinant MRC11 [antigenically identical to the strain A/Port Chalmers/1/73 (H3N2)] by bromelain treatment and purified by ... | [
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PMID:25003 | Effect of 5-bromo-2'-deoxyuridine on the in vitro DNA synthesizing activity of polyoma virus-infected cells. | The in vitro synthesizing activity of uninfected and polyoma virus-infected mouse embryo (ME) cells was investigated by measuring the incorporation of 3H-thymidine (dTR) into acidinsoluble material in cell-free extracts. The character of the enzyme reaction was described in uninfected cells and an activating effect of ... | Effect of 5-bromo-2'-deoxyuridine on the in vitro DNA synthesizing activity of polyoma virus-infected cells. The in vitro synthesizing activity of uninfected and polyoma virus-infected mouse embryo (ME) cells was investigated by measuring the incorporation of 3H-thymidine (dTR) into acidinsoluble material in cell-free ... | [
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PMID:25004 | Some properties of the adenosine triphosphatase associated with herpes simplex virus and nuclear membrane of host cells. | An enzyme capable to split adenosine triphosphate (ATP) was shown to be firmly associated with mature herpes simplex virus particles purified from infected rabbit lung (ZP) cells. The enzyme localized in the viral envelope was markedly activated by bivalent cations, to the largest degree by Mg2+ at a pH optimum of 7.8-... | Some properties of the adenosine triphosphatase associated with herpes simplex virus and nuclear membrane of host cells. An enzyme capable to split adenosine triphosphate (ATP) was shown to be firmly associated with mature herpes simplex virus particles purified from infected rabbit lung (ZP) cells. The enzyme localize... | [
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PMID:25005 | Dengue viral antigens in host cell membranes. | Membranes from type 2 dengue virus (DEN-2) infected BHK cells, when used as immunogen, elicit antibodies detectable by complement fixation, hemagglutination inhibition and plaque reduction neutralization tests with suckling-mouse-brain-derived DEN-antigens. Membrane fractions from infected cells separated by sucrose de... | Dengue viral antigens in host cell membranes. Membranes from type 2 dengue virus (DEN-2) infected BHK cells, when used as immunogen, elicit antibodies detectable by complement fixation, hemagglutination inhibition and plaque reduction neutralization tests with suckling-mouse-brain-derived DEN-antigens. Membrane fractio... | [
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PMID:25006 | Studies of cell-mediated immunity to Junín virus. | Junín virus is uniformly lethal in newborn mice, but fails to kill adult animals. But there is direct evidence that in older mice the immunological mechanism occurs when certain conditions are fulfilled. The present results showed that in Junín virus infection of mice, the development of the immunological mechanism occ... | Studies of cell-mediated immunity to Junín virus. Junín virus is uniformly lethal in newborn mice, but fails to kill adult animals. But there is direct evidence that in older mice the immunological mechanism occurs when certain conditions are fulfilled. The present results showed that in Junín virus infection of mice, ... | [
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PMID:25007 | Antiviral pyrimidine derivatives: binding with biopolymers and photosensitization. | Two antiviral pyrimidine derivatives, known to partially inhibit RNA dependent RNA polymerase of Mengo virus in a cell-free system, were found to bind with biopolymers. The antiviral compounds, at concentration of 100 and 50 muM and lower, showed complete inhibition of the virus-induced cytopathic effect and plaque red... | Antiviral pyrimidine derivatives: binding with biopolymers and photosensitization. Two antiviral pyrimidine derivatives, known to partially inhibit RNA dependent RNA polymerase of Mengo virus in a cell-free system, were found to bind with biopolymers. The antiviral compounds, at concentration of 100 and 50 muM and lowe... | [
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0.0... |
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