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PMID:26823 | [Possibility of using higher plants in a life-support system on the moon]. | The paper discusses the possibility of repeated termination of plant vegetation by prolonged darkness approximating the lunar night. This may be helpful for the incorporation of higher plants into the life support system of lunar bases, the solar light being used for illumination. In this connection vegetables (beet Bo... | [Possibility of using higher plants in a life-support system on the moon]. The paper discusses the possibility of repeated termination of plant vegetation by prolonged darkness approximating the lunar night. This may be helpful for the incorporation of higher plants into the life support system of lunar bases, the sola... | [
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PMID:26831 | The effect of phospholipase D on the function of fragmented sarcoplasmic reticulum. | Incubations of fresh preparations of fragmented sarcoplasmic reticulum (FSR) were carried out at pH 5.7. This pH was necessary for hydrolysis of phospholipids by phospholipase D. The pH did not influence calcium uptake or the activity of calcium-stimulated ATPase of FSR. Treatment of FSR with phospholipase D caused hyd... | The effect of phospholipase D on the function of fragmented sarcoplasmic reticulum. Incubations of fresh preparations of fragmented sarcoplasmic reticulum (FSR) were carried out at pH 5.7. This pH was necessary for hydrolysis of phospholipids by phospholipase D. The pH did not influence calcium uptake or the activity o... | [
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PMID:26832 | Multiple forms of acid phosphatase activity in Gaucher's disease. | Although the primary genetic defect in all individuals with Gaucher's disease is a deficiency in glucocerebrosidase activity, the finding of marked elevations in splenic and serum acid phosphatase activity is almost as consistent a finding. Gaucher spleen and serum contain at least two forms of acid phosphatase that ca... | Multiple forms of acid phosphatase activity in Gaucher's disease. Although the primary genetic defect in all individuals with Gaucher's disease is a deficiency in glucocerebrosidase activity, the finding of marked elevations in splenic and serum acid phosphatase activity is almost as consistent a finding. Gaucher splee... | [
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PMID:26851 | [Thermostability of glutamine synthetase in Candida tropicalis feed yeasts in Mg-, Mn- and Co-activated systems]. | The thermostability of glutamine synthetase of Candida tropicalis was studied in systems activated by Mg, Mn and Co. The enzyme was found to be very thermolabile. Its thermostability depended on the nature of a bivalent cation used in the reaction mixture. Metals stabilized the enzyme since preincubation with metal cat... | [Thermostability of glutamine synthetase in Candida tropicalis feed yeasts in Mg-, Mn- and Co-activated systems]. The thermostability of glutamine synthetase of Candida tropicalis was studied in systems activated by Mg, Mn and Co. The enzyme was found to be very thermolabile. Its thermostability depended on the nature ... | [
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PMID:26852 | [Effect of the composition of the medium and the conditions of Aspergillus foetidus cultivation on the biosynthesis of glucoamylase]. | The optimal conditions for biosynthesis of exocellular glucoamylase were found in the course of submerged cultivation of Aspergillus foetidus ATCC 14916: pH 4.5 at the beginning of cultivation, cultivation for four days, a temperature of 30 degrees C, and an aeration in the fermenter of 1.5 volumes of the air per 1 vol... | [Effect of the composition of the medium and the conditions of Aspergillus foetidus cultivation on the biosynthesis of glucoamylase]. The optimal conditions for biosynthesis of exocellular glucoamylase were found in the course of submerged cultivation of Aspergillus foetidus ATCC 14916: pH 4.5 at the beginning of culti... | [
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PMID:26853 | [Cellulase formation by soil yeasts of the genus Trichosporon and by microscopic fungi]. | The production of celulases by a soil yeast belonging to the genus Trichosporon and by microscopic fungi of various systematic groups was studied. The yeast synthesized relatively small amounts of cellulases in contrast to microscopic fungi. The active culture producing cellulases was the fungus Trichoderma longibrachi... | [Cellulase formation by soil yeasts of the genus Trichosporon and by microscopic fungi]. The production of celulases by a soil yeast belonging to the genus Trichosporon and by microscopic fungi of various systematic groups was studied. The yeast synthesized relatively small amounts of cellulases in contrast to microsco... | [
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PMID:26855 | [Cultivation of feed yeasts on media with carbohydrates]. | The different culture of yeasts oxidizing hydrocarbons and the accompanying microflora are, under certain physico-chemical conditions, in metabiotic relationships which provide stability of the system. These relationships were studied with yeast cultures belonging to the Candida genus and oxidizing hydrocarbons. The re... | [Cultivation of feed yeasts on media with carbohydrates]. The different culture of yeasts oxidizing hydrocarbons and the accompanying microflora are, under certain physico-chemical conditions, in metabiotic relationships which provide stability of the system. These relationships were studied with yeast cultures belongi... | [
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PMID:26856 | [Origin of adifferentiated variants of Streptomyces roseoflavus in submerged culture]. | Adifferentiated Nocardia-like variants (Nv) of Streptomyces roseoflavus var. roseofungini, similar in their cultural characteristics with the variant 1-68 described elsewhere (Nikitina, 1968; Kalakoutskii a. Nikitina, 1976), can be obtained not only in the course of growth of the parent culture on a solid medium contai... | [Origin of adifferentiated variants of Streptomyces roseoflavus in submerged culture]. Adifferentiated Nocardia-like variants (Nv) of Streptomyces roseoflavus var. roseofungini, similar in their cultural characteristics with the variant 1-68 described elsewhere (Nikitina, 1968; Kalakoutskii a. Nikitina, 1976), can be o... | [
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PMID:26857 | [Effectiveness of strains of clover nodule bacteria adapted to the acid reaction of the soil]. | The effectiveness and virulence of clover nodule bacterial strains of different origin was studied as they were adapted to acid pH (4.8) of soil in the course of seven years. In spite of their ecological adaptation (strains from acid and neutral soils), the majority of strains (10 among 20) grew well enough upon numero... | [Effectiveness of strains of clover nodule bacteria adapted to the acid reaction of the soil]. The effectiveness and virulence of clover nodule bacterial strains of different origin was studied as they were adapted to acid pH (4.8) of soil in the course of seven years. In spite of their ecological adaptation (strains f... | [
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PMID:26863 | Isoenzymes of human liver alpha-L-fucosidase: chemical relationship, kinetic studies, and immunochemical characterization. | The chemical relationship of the seven forms of human liver alpha-L fucosidase has been studied by isoelectric focusing of neuraminidase- and sialytransferase-treated preparations of alpha-L-fucosidase. Neuraminidase treatment leads to a decrease in the activity of the more acidic forms (IV-VII) and a concimitant incre... | Isoenzymes of human liver alpha-L-fucosidase: chemical relationship, kinetic studies, and immunochemical characterization. The chemical relationship of the seven forms of human liver alpha-L fucosidase has been studied by isoelectric focusing of neuraminidase- and sialytransferase-treated preparations of alpha-L-fucosi... | [
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PMID:26866 | [On the pharmacological treatment of acute spasmodic laryngitis (Pseudo-Croup) (author's transl)]. | Three aspects of the pharmacological treatment of pseudo-croup are discussed in this article. Sedatives should be given only when close clinical supervision is guaranteed. Otherwise the apparent drowsiness observed in patients passing from stage III to stage IV of the disease may be masked or misinterpreted due to thes... | [On the pharmacological treatment of acute spasmodic laryngitis (Pseudo-Croup) (author's transl)]. Three aspects of the pharmacological treatment of pseudo-croup are discussed in this article. Sedatives should be given only when close clinical supervision is guaranteed. Otherwise the apparent drowsiness observed in pat... | [
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PMID:26867 | [Atrial tachycardia with atrio-ventricular block in infancy and childhood (author's transl)]. | Atrial tachycardia with atrio-ventricular block is a very rare arrhythmia in infancy and childhood. We saw ten patients between 3 days and fifteen years old. Six of them had a congenital heart disease, in four cases we could'nt find any cardiac or extracardiac illness. In every case digitalis intoxication was excluded.... | [Atrial tachycardia with atrio-ventricular block in infancy and childhood (author's transl)]. Atrial tachycardia with atrio-ventricular block is a very rare arrhythmia in infancy and childhood. We saw ten patients between 3 days and fifteen years old. Six of them had a congenital heart disease, in four cases we could'n... | [
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PMID:26865 | [Electrical conductivity of isoionic DNA solutions]. | Conductance of the DNA isoionic solutions obtained from aqueous solutions of its sodium salt using a column with mixed bed ionexchangers has been investigated. It has been found that in Kohlrausch's plots equivalent conductance slightly decreased with polymer concentration. Comparison of the degree of ionization (alpha... | [Electrical conductivity of isoionic DNA solutions]. Conductance of the DNA isoionic solutions obtained from aqueous solutions of its sodium salt using a column with mixed bed ionexchangers has been investigated. It has been found that in Kohlrausch's plots equivalent conductance slightly decreased with polymer concent... | [
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PMID:26870 | [Treatment of hypertension with a combination of pyridopyridazine derivative with a beta-receptor blocker. Studies on the hemodynamic course (author's transl)]. | 22 pateints with a fixed, sometimes therapy-resistant (N = 18) hypertension were treated with a new antihypertensive agent, the pyridopyridazine derivative BQ 22-708 and hemodynamically investigated. 14 hypertensives received single oral doses of between 5 and 15 mg, 8 other patients also received additional treatment ... | [Treatment of hypertension with a combination of pyridopyridazine derivative with a beta-receptor blocker. Studies on the hemodynamic course (author's transl)]. 22 pateints with a fixed, sometimes therapy-resistant (N = 18) hypertension were treated with a new antihypertensive agent, the pyridopyridazine derivative BQ ... | [
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PMID:26871 | [Cardiac neurosis in everyday general medical practice (author's transl)]. | From results of the study of 37 patients with cardiac neurosis, new conceptual relationships to explain this condition are developed. Cardiac neurosis is explained as a disturbance of the "psycho-neurovegetative self-regulation mechanism" (servomechanisms). The authors show how such a conception, which has been borrowe... | [Cardiac neurosis in everyday general medical practice (author's transl)]. From results of the study of 37 patients with cardiac neurosis, new conceptual relationships to explain this condition are developed. Cardiac neurosis is explained as a disturbance of the "psycho-neurovegetative self-regulation mechanism" (servo... | [
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PMID:26872 | Variable content of histaminase, L-dopa decarboxylase and calcitonin in small-cell carcinoma of the lung. Biologic and clinical implications. | To ascertain whether the content of endocrine markers is constant in small-cell carcinoma of the lung, levels of three markers of medullary thyroid carcinoma were studied in this tumor. Histaminase was increased in six of six primary tumors (three to 14,000 times), L-dopa decarboxylase in four of six (six to 30 times),... | Variable content of histaminase, L-dopa decarboxylase and calcitonin in small-cell carcinoma of the lung. Biologic and clinical implications. To ascertain whether the content of endocrine markers is constant in small-cell carcinoma of the lung, levels of three markers of medullary thyroid carcinoma were studied in this... | [
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PMID:26878 | Current problems in the control of mosquitoes. | Since the incidence of mosquito-borne disease was drastically reduced by the use of DDT in the 1950s, the problem has again worsened and no single method of eradication seems likely to achieve lasting success. It now seems that the best hope lies in the combination of various methods of chemical control and perhaps gen... | Current problems in the control of mosquitoes. Since the incidence of mosquito-borne disease was drastically reduced by the use of DDT in the 1950s, the problem has again worsened and no single method of eradication seems likely to achieve lasting success. It now seems that the best hope lies in the combination of vari... | [
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PMID:26888 | [Value of brain metabolic studies in post-traumatic coma]. | In 34 comatose patients in the acute phase, the mean hemispheric CBF is lowered as well as the CMRO2, with a quite good relation between these values and the coma level and prognostic so. The cerebral response to a PaCO2 range indicates a quite good relation with the coma level (the lowest value in the most severe coma... | [Value of brain metabolic studies in post-traumatic coma]. In 34 comatose patients in the acute phase, the mean hemispheric CBF is lowered as well as the CMRO2, with a quite good relation between these values and the coma level and prognostic so. The cerebral response to a PaCO2 range indicates a quite good relation wi... | [
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PMID:26891 | [Alkaline urine in the aged]. | Considering the frequent finding of alkalinity in the urines of elderly people of both sexes, we can propose many hypothesis among which the most probable seems a latent renal failure with secondary hyperparathyroidism and consequent alkalinization of the urines. We can presume that such a frequent alkalinity of the ur... | [Alkaline urine in the aged]. Considering the frequent finding of alkalinity in the urines of elderly people of both sexes, we can propose many hypothesis among which the most probable seems a latent renal failure with secondary hyperparathyroidism and consequent alkalinization of the urines. We can presume that such a... | [
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PMID:26893 | [Hemodynamic effects of antihypertensive drugs]. | The physiopathology of hypertension is not fully understood, though the haemodynamic pictures accompanying the various arterial forms have been established. Clinical employment of antihypertensive drugs should tend to correct these pictures and the extent to which this is true is examined in the light of the latest evi... | [Hemodynamic effects of antihypertensive drugs]. The physiopathology of hypertension is not fully understood, though the haemodynamic pictures accompanying the various arterial forms have been established. Clinical employment of antihypertensive drugs should tend to correct these pictures and the extent to which this i... | [
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PMID:26895 | Urinary sulfate excretion in children with classic renal tubular acidosis. | These studies quantitat the significantly elevated sulfate excretion in the urine of children with classic renal tubular acidosis as compared to six weight-matched controls (1.4 +/-0.5 vs. 0.7 +/- 0.2 mEq/kg/day;p less than 0.05). This sulfate loss may result in a subclinical sulfate deficiency which may contribute to ... | Urinary sulfate excretion in children with classic renal tubular acidosis. These studies quantitat the significantly elevated sulfate excretion in the urine of children with classic renal tubular acidosis as compared to six weight-matched controls (1.4 +/-0.5 vs. 0.7 +/- 0.2 mEq/kg/day;p less than 0.05). This sulfate l... | [
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PMID:26896 | Hydrogen ion production secondary to metabolism of sulfur-amino acids and organic acids. | The rate of acid production in infants and young children was quoted to be 1-2 mEq/kg/day but in fact, the sole reference used in all these instances did not permit such quantitation. In order to evaluate the rate of endogenous acid production, we applied the quantitative technique of Lennon et al. in the following nor... | Hydrogen ion production secondary to metabolism of sulfur-amino acids and organic acids. The rate of acid production in infants and young children was quoted to be 1-2 mEq/kg/day but in fact, the sole reference used in all these instances did not permit such quantitation. In order to evaluate the rate of endogenous aci... | [
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PMID:26899 | The effect of atropine on the lower esophageal sphincter in late pregnancy. | Intraluminal gastroesophageal pressure and pH studies have been performed on 8 nonpregnant women, 10 pregnant women with heartburn, and 10 pregnant women without heartburn. Each patient was tested under resting conditions and after intravenous injection of 0.6 mg atropine. In both groups of pregnant patients the intrag... | The effect of atropine on the lower esophageal sphincter in late pregnancy. Intraluminal gastroesophageal pressure and pH studies have been performed on 8 nonpregnant women, 10 pregnant women with heartburn, and 10 pregnant women without heartburn. Each patient was tested under resting conditions and after intravenous ... | [
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PMID:26900 | Deceleration/contraction ratios as an index of fetal health during labor. | Internal FHR tracings of 259 patients with variable decelerations and 49 with late decelerations were analyzed for frequency and severity of the pattern in an attempt to correlate with fetal pH or Apgar score at 1 minute. A significant increase in the risk of fetal acidosis was associated with a frequency of greater th... | Deceleration/contraction ratios as an index of fetal health during labor. Internal FHR tracings of 259 patients with variable decelerations and 49 with late decelerations were analyzed for frequency and severity of the pattern in an attempt to correlate with fetal pH or Apgar score at 1 minute. A significant increase i... | [
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PMID:26901 | gamma-Glutamyl transpeptidase activity in sheep serum: normal values and an evaluation of its potential for detecting liver involvement in experimental lupinosis. | A brief survey of the literature on gamma-glutamyl transpeptidase (gamma-GT) activity is included in this study. The levels of activity in the serum of normal Merino sheep (13,6-32,4 mI.U/ml) were ascertained as a preliminary to following the activity through the entire course of experimentally induced ovine lupinosis,... | gamma-Glutamyl transpeptidase activity in sheep serum: normal values and an evaluation of its potential for detecting liver involvement in experimental lupinosis. A brief survey of the literature on gamma-glutamyl transpeptidase (gamma-GT) activity is included in this study. The levels of activity in the serum of norma... | [
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PMID:26902 | The metabolic profile of adult Fasciola hepatica obtained from rafoxanide-treated sheep. | Sheep infected with adult Fasciola hepatica were drenched with rafoxanide. At 4, 8, 16 and 24 h after drenching the sheep were killed and the flukes removed, washed and rapidly frozen in liquid nitrogen. The content of key metabolites in the fermentation pathway were determined and compared with those in control F. hep... | The metabolic profile of adult Fasciola hepatica obtained from rafoxanide-treated sheep. Sheep infected with adult Fasciola hepatica were drenched with rafoxanide. At 4, 8, 16 and 24 h after drenching the sheep were killed and the flukes removed, washed and rapidly frozen in liquid nitrogen. The content of key metaboli... | [
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PMID:26908 | A basis for migraine therapy- the autonomic theory reappraised. | The concept that migraine results from an initial vasoconstriction due to increased release of noradrenaline from the sympathetic nerves to cranial blood vessels has been reappraised in the light of recently acquired knowledge of the mechanisms of action of drugs used in the treatment of migraine, physiological and pha... | A basis for migraine therapy- the autonomic theory reappraised. The concept that migraine results from an initial vasoconstriction due to increased release of noradrenaline from the sympathetic nerves to cranial blood vessels has been reappraised in the light of recently acquired knowledge of the mechanisms of action o... | [
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PMID:26910 | [Changes in the urinary excretion of gamma-glutamyltranspeptidase, leucine aminopeptidas and alkaline phosphatase in the combined action of ethylene glycol and high temperature]. | Gamma-glutamyl transpeptidase (gamma--GTP), leucine aminopeptidase (LAP) and alkaline phosphatase (AP) excretion in rats is followed in dynamics (2, 8, 15, 30 and 90 days) upon isolated and combined treatment with ethylene glycol (EG) at dose 1/8 LD50 and temperature of the environment 35 degrees C. Under the effect of... | [Changes in the urinary excretion of gamma-glutamyltranspeptidase, leucine aminopeptidas and alkaline phosphatase in the combined action of ethylene glycol and high temperature]. Gamma-glutamyl transpeptidase (gamma--GTP), leucine aminopeptidase (LAP) and alkaline phosphatase (AP) excretion in rats is followed in dynam... | [
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PMID:26912 | Mode of action of colicin Ia: effect of colicin on the Escherichia coli proton electrochemical gradient. | By use of the technique of flow dialysis, the membrane potential (deltapsi) and pH gradient (deltapH) have been measured in colicin Ia-treated Escherichia coli K-12 cells and in membrane vesicles prepared from such cells. Although such cells and vesicles are able to generate a transmembrane deltapH at pH 5.5, they do n... | Mode of action of colicin Ia: effect of colicin on the Escherichia coli proton electrochemical gradient. By use of the technique of flow dialysis, the membrane potential (deltapsi) and pH gradient (deltapH) have been measured in colicin Ia-treated Escherichia coli K-12 cells and in membrane vesicles prepared from such ... | [
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PMID:26913 | Proposed temperature-dependent conformational transition in D-amino acid oxidase: a differential scanning microcalorimetric study. | A number of authors have reported observations on D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3.] that they have interpreted in terms of a temperature-dependent conformational transition having a van't Hoff enthalpy amounting to more than 1 cal per g of protein (1 cal = 4.184J). No ind... | Proposed temperature-dependent conformational transition in D-amino acid oxidase: a differential scanning microcalorimetric study. A number of authors have reported observations on D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3.] that they have interpreted in terms of a temperature-depe... | [
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PMID:26914 | Elimination of cooperativity in aspartate transcarbamylase by nitration of a single tyrosine residue. | In a previous report [Landfear, S. M., Lipscomb, W. N. & Evans, D.R. (1978) J. Biol. Chem. 253, 3988--3996] we demonstrated that tetranitromethane can be employed to nitrate a limited number of tyrosine residues in aspartate transcarbamylase (carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2); such mo... | Elimination of cooperativity in aspartate transcarbamylase by nitration of a single tyrosine residue. In a previous report [Landfear, S. M., Lipscomb, W. N. & Evans, D.R. (1978) J. Biol. Chem. 253, 3988--3996] we demonstrated that tetranitromethane can be employed to nitrate a limited number of tyrosine residues in... | [
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PMID:26915 | Heat-stable enterotoxin of Escherichia coli: in vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine. | A partially purified preparation of the heat-stable enterotoxin of Escherichia coli caused a rapid and persistent increase in electric potential difference and short-circuit current when added in vitro to the luminal surface of isolated rabbit ileal mucosa. As little as 1 ng/ml produced an easily detectable response. U... | Heat-stable enterotoxin of Escherichia coli: in vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine. A partially purified preparation of the heat-stable enterotoxin of Escherichia coli caused a rapid and persistent increase in electric potential difference and sho... | [
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PMID:26916 | Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins. | Mouse bone marrow and spleen cells were fractionated with the aid of soybean agglutinin and peanut agglutinin. A test for spleen colony-forming units in the isolated fractions showed that the hemopoietic stem cells are agglutinated by both of these lectins. The capacity of the agglutinated fractions to reconstitute let... | Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins. Mouse bone marrow and spleen cells were fractionated with the aid of soybean agglutinin and peanut agglutinin. A test for spleen colony-forming units in the isolated fractions showed that the hemopoietic stem cells a... | [
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PMID:26917 | Beneficial and detrimental actions of histamine H1- and H2-receptor antagonists in circulatory shock. | This study explores the use of both histamine H(1)- and H(2)-receptor antagonists in two different forms of circulatory shock and suggests that histamine may be involved in more than one way in the pathophysiology of circulatory shock. Various single doses of diphenhydramine, chlorpheniramine, promethazine, and burimam... | Beneficial and detrimental actions of histamine H1- and H2-receptor antagonists in circulatory shock. This study explores the use of both histamine H(1)- and H(2)-receptor antagonists in two different forms of circulatory shock and suggests that histamine may be involved in more than one way in the pathophysiology of c... | [
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PMID:26918 | Biochemical and immunochemical study of lysyl oxidase in experimental hepatic fibrosis in the rat. | Lysyl oxidase catalyzes the crosslinking of collagen and elastin. Lysyl oxidase activity was measured and localized in rat liver during the evolution of hepatic fibrosis induced by CCl4. Enzyme activity measured with DL-[6-3H]-lysine-labeled collagen substrates in liver and plasma increased sharply after approximately ... | Biochemical and immunochemical study of lysyl oxidase in experimental hepatic fibrosis in the rat. Lysyl oxidase catalyzes the crosslinking of collagen and elastin. Lysyl oxidase activity was measured and localized in rat liver during the evolution of hepatic fibrosis induced by CCl4. Enzyme activity measured with DL-[... | [
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PMID:26919 | Ontogenetic appearance and disappearance of tyrosine hydroxylase and catecholamines in the rat embryo. | The ontogenetic pattern of noradrenergic differentiation in rat embryonic autonomic neuroblasts was defined in vivo. Noradrenergic specialization was examined by documenting the immunohistochemical appearance of tyrosine hydroxylase [Tyr-OH; tyrosine 3-monooxygenase; L-tyrosine,-tetrahydropteridine:oxygen oxidoreductas... | Ontogenetic appearance and disappearance of tyrosine hydroxylase and catecholamines in the rat embryo. The ontogenetic pattern of noradrenergic differentiation in rat embryonic autonomic neuroblasts was defined in vivo. Noradrenergic specialization was examined by documenting the immunohistochemical appearance of tyros... | [
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PMID:26925 | Bacteriorhodopsin: lipid environment and conformational changes. | The polar lipids of the purple membrane were exchanged for different phosphatidylcholine species. The resulting complexes had the same protein to lipid-phosphorus ratio as the natural membrane, but only about 0.5-1.0 mole of original lipid was still present per mole of bacteriorhodopsin. In such complexes the bacterior... | Bacteriorhodopsin: lipid environment and conformational changes. The polar lipids of the purple membrane were exchanged for different phosphatidylcholine species. The resulting complexes had the same protein to lipid-phosphorus ratio as the natural membrane, but only about 0.5-1.0 mole of original lipid was still prese... | [
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PMID:26926 | Proteins containing reductively aminated disaccharides: chemical and immunochemical characterization. | Synthetic glycoproteins can be prepared by reductive amination of proteins and reducing carbohydrates in the presence of sodium cyanoborohydride. The reaction proceeds readily in aqueous solution at pH 6--9 to give high degrees of substitution. The degree of substitution can be determined by amino acid analysis, as the... | Proteins containing reductively aminated disaccharides: chemical and immunochemical characterization. Synthetic glycoproteins can be prepared by reductive amination of proteins and reducing carbohydrates in the presence of sodium cyanoborohydride. The reaction proceeds readily in aqueous solution at pH 6--9 to give hig... | [
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PMID:26933 | Intruder-evoked aggression in isolated and nonisolated mice: effects of psychomotor stimulants and L-dopa. | Adult male Swiss-Webster mice were housed either singly (isolated) or with a female (nonisolated). Aggressive behavior was evoked by introducing a group-housed male mouse (intruder) into the home cage of the isolated or nonisolated mouse (resident).d-Amphetamine, methamphetamine, methylphenidate, cocaine, and L-dopa de... | Intruder-evoked aggression in isolated and nonisolated mice: effects of psychomotor stimulants and L-dopa. Adult male Swiss-Webster mice were housed either singly (isolated) or with a female (nonisolated). Aggressive behavior was evoked by introducing a group-housed male mouse (intruder) into the home cage of the isola... | [
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PMID:26942 | Effect of thoracentesis on arterial oxygen tension. | The acute effects of thoracentesis on arterial oxygenation were observed in 19 studies on 17 patients. Arterial blood samples were obtained prior to, immediately after, and 1 h after thoracentesis. Unpredictable changes in the arterial oxygen tension, ranging from -22 to +19 mm Hg were found immediately following thora... | Effect of thoracentesis on arterial oxygen tension. The acute effects of thoracentesis on arterial oxygenation were observed in 19 studies on 17 patients. Arterial blood samples were obtained prior to, immediately after, and 1 h after thoracentesis. Unpredictable changes in the arterial oxygen tension, ranging from -22... | [
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PMID:26943 | Mean whole body intracellular pH in unanesthetized dogs: a revised method. | An improved method of calculating the mean whole body intracellular pH (pHi) by means of DMO in unanesthetized dogs is described. The elemination of DMO from the body fluid is assumed to follow a simple exponential decay with a time constant k1. The distribution of DMO into the extracellular and intracellular water is ... | Mean whole body intracellular pH in unanesthetized dogs: a revised method. An improved method of calculating the mean whole body intracellular pH (pHi) by means of DMO in unanesthetized dogs is described. The elemination of DMO from the body fluid is assumed to follow a simple exponential decay with a time constant k1.... | [
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PMID:26944 | The influence of low, normal and high Pa(O2) on the respiratory effects of Pa(CO2) oscillations in anaesthetised cats. | The present study examines the influence of oxygen tension on the recurrent tidal volume changes caused by oscillations in arterial P(CO2). The oscillations have a period equal to two respiratory cycles, and are produced by giving alternately CO2 -enriched and CO2 -free gas mixtures. The results show alternation of the... | The influence of low, normal and high Pa(O2) on the respiratory effects of Pa(CO2) oscillations in anaesthetised cats. The present study examines the influence of oxygen tension on the recurrent tidal volume changes caused by oscillations in arterial P(CO2). The oscillations have a period equal to two respiratory cycle... | [
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PMID:26945 | Respiratory characteristics of opossum (Didelphis virginiana) blood during chronic anemia. | In five adult opossums (Didelphis virginiana), blood hemoglobin concentration was lowered from 12.3g% to 6.4g% by daily bleeding. Blood P50 rose significantly (P is less than 0.05) from 44.5 mm Hg to 47.4 mm Hg and the concentration of 2, 3-diphosphoglycerate (DPG) increased from 14.17 mumol/g Hb to 20.90 mumol/g Hb. T... | Respiratory characteristics of opossum (Didelphis virginiana) blood during chronic anemia. In five adult opossums (Didelphis virginiana), blood hemoglobin concentration was lowered from 12.3g% to 6.4g% by daily bleeding. Blood P50 rose significantly (P is less than 0.05) from 44.5 mm Hg to 47.4 mm Hg and the concentrat... | [
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PMID:26946 | Oxygen transport in chicken embryos under hypothermal exposure. | Fertile hens' eggs incubated for 16 days at 38 degrees C were abruptly exposed to a temperature of 30 degrees C for 2 h and then determinations of blood gas parameters and the O2 dissociation curve were performed. In addition, O2 uptake was measured. Under hypothermal exposure the O2 dissociation curve showed a marked ... | Oxygen transport in chicken embryos under hypothermal exposure. Fertile hens' eggs incubated for 16 days at 38 degrees C were abruptly exposed to a temperature of 30 degrees C for 2 h and then determinations of blood gas parameters and the O2 dissociation curve were performed. In addition, O2 uptake was measured. Under... | [
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PMID:26947 | Comparison of efflux rates of hydrogen and lactate ions from isolated muscles in vitro. | Relative rates of efflux of hydrogen and lactate ions from skeletal muscle in vitro were determined on isolated rat diaphragms and frog satorius muscles. After a period of lacate accumulation by stimulation in vitro, muscles were suspended in a small volume of Ringer solution for different time periods lasting up to 60... | Comparison of efflux rates of hydrogen and lactate ions from isolated muscles in vitro. Relative rates of efflux of hydrogen and lactate ions from skeletal muscle in vitro were determined on isolated rat diaphragms and frog satorius muscles. After a period of lacate accumulation by stimulation in vitro, muscles were su... | [
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PMID:26965 | [Détermination of salivary pH by contact pH metry. Study of ph on the tongue and of the orifices of Wharton's and Stenon's ducts]. | The authors describe an electrochemical technique for the measurement of salivary pH by contact. They present a critical study fo 167 cases. The values obtained are characteristic in terms of age and the site of determination (dorsal surface of the tongue, orifice of Wharton's or Stenon's ducts). | [Détermination of salivary pH by contact pH metry. Study of ph on the tongue and of the orifices of Wharton's and Stenon's ducts]. The authors describe an electrochemical technique for the measurement of salivary pH by contact. They present a critical study fo 167 cases. The values obtained are characteristic in terms ... | [
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PMID:26966 | The electrical potential difference and impedance between CSF and blood in unanesthetized man. | The electrical potential difference (PD) between cerebrospinal fluid (CSF) and blood has been studied extensively in animals. To obtain data from man the PD and impedance were recorded from the lumbar subarachnoid space of thirteen unanaesthetized patients with a presumably normal blood--brain barrier. The spontaneous ... | The electrical potential difference and impedance between CSF and blood in unanesthetized man. The electrical potential difference (PD) between cerebrospinal fluid (CSF) and blood has been studied extensively in animals. To obtain data from man the PD and impedance were recorded from the lumbar subarachnoid space of th... | [
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PMID:26968 | A short duration renal acidification test. | As earlier investigations of renal acidification capacity were poorly standardized it was considered important to develop a clinically useful method. The ability of the kidneys to acidify urine was studied in 19 normal persons by short ammonium chloride loading. Ammonium chloride was given in a dose of 150 mmol/m2 body... | A short duration renal acidification test. As earlier investigations of renal acidification capacity were poorly standardized it was considered important to develop a clinically useful method. The ability of the kidneys to acidify urine was studied in 19 normal persons by short ammonium chloride loading. Ammonium chlor... | [
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PMID:26969 | Urine acidification capacity in renal stone formers. | A short ammonium chloride loading test was used in the investigation of renal acidification capacity of 51 patients with idiopathic recurrent renal stones containing calcium. Many of these patients had suffered from prolonged and severe stone disease. In 31% of the patients an impaired acidification with varying degree... | Urine acidification capacity in renal stone formers. A short ammonium chloride loading test was used in the investigation of renal acidification capacity of 51 patients with idiopathic recurrent renal stones containing calcium. Many of these patients had suffered from prolonged and severe stone disease. In 31% of the p... | [
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PMID:26970 | Bicarbonate re-absorption in renal stone patients with acidification defects. | A pathological renal response to ammonium chloride loading was found to be common in renal stone formers. In order to elicit further information about the impaired acidification capacity eight patients were investigated by bicarbonate loading. One patient with a mild acidification defect had normal tubular re-absorptio... | Bicarbonate re-absorption in renal stone patients with acidification defects. A pathological renal response to ammonium chloride loading was found to be common in renal stone formers. In order to elicit further information about the impaired acidification capacity eight patients were investigated by bicarbonate loading... | [
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PMID:26972 | Immunofluorescent detection of nuclear double-stranded RNA in situ in Vero and mosquito cells. | Double-stranded RNA (dsRNA) was detected in situ by indirect immunofluorescence with antibodies to dsRNA. It was seen in nuclei of Vero and Aedes albopictus cells, but not in BHK cells, KB cells, chick embryo fibroblasts, or HeLa cells. Reactive dsRNA was present in the nucleoplasm, but not in nucleoli or cytoplasm. Ex... | Immunofluorescent detection of nuclear double-stranded RNA in situ in Vero and mosquito cells. Double-stranded RNA (dsRNA) was detected in situ by indirect immunofluorescence with antibodies to dsRNA. It was seen in nuclei of Vero and Aedes albopictus cells, but not in BHK cells, KB cells, chick embryo fibroblasts, or ... | [
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PMID:26973 | Sodium channel inactivation in squid axon is removed by high internal pH or tyrosine-specific reagents. | In squid axon, internal alkalinization from pH 7.1 to pH 10.2 results in a reversible decrease of the maximum inward current and the steady state sodium channel inactivation. Similar effects were observed after treatment of the axon with tetranitromethane or after iodination with lactoperoxidase. These results suggest ... | Sodium channel inactivation in squid axon is removed by high internal pH or tyrosine-specific reagents. In squid axon, internal alkalinization from pH 7.1 to pH 10.2 results in a reversible decrease of the maximum inward current and the steady state sodium channel inactivation. Similar effects were observed after treat... | [
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PMID:26974 | Histamine H1 receptor-mediated guanosine 3',5'-monophosphate formation by cultured mouse neuroblastoma cells. | Incubation of cultured mouse neuroblastoma cells with histamine caused a rapid and marked increase in the formation of guanosine 3',5'-monophosphate (cyclic GMP) by these cells. Receptor agonists for H1, but not H2, caused this effect which was reduced by H1 but not by H2 or muscarinic acetylcholine receptor antagonist... | Histamine H1 receptor-mediated guanosine 3',5'-monophosphate formation by cultured mouse neuroblastoma cells. Incubation of cultured mouse neuroblastoma cells with histamine caused a rapid and marked increase in the formation of guanosine 3',5'-monophosphate (cyclic GMP) by these cells. Receptor agonists for H1, but no... | [
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PMID:26975 | Long-term changes in dopaminergic innervation of caudate nucleus after continuous amphetamine administration. | Silicone pellets containing d-amphetamine base were implanted subcutaneously in rats. These pellets release amphetamine continuously for at least 10 days. Several days after implantation, swollen dopamine axons concomitant with large decreases in tyrosine hydroxylase activity were observed in the caudate nucleus. Decre... | Long-term changes in dopaminergic innervation of caudate nucleus after continuous amphetamine administration. Silicone pellets containing d-amphetamine base were implanted subcutaneously in rats. These pellets release amphetamine continuously for at least 10 days. Several days after implantation, swollen dopamine axons... | [
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PMID:26976 | Kainic acid lesions of the striatum dissociate amphetamine and apomorphine stereotypy: similarities to Huntingdon's chorea. | Kainic acid lesion of cell bodies in the dorsal striatum enhanced the stereotypy-producing effects of d-amphetamine without affecting the sterotypy produced by the direct receptor agonist apomorphine. This pattern of results parallels that found in patients suffering from Hungtington's chorea, thus strengthening the pa... | Kainic acid lesions of the striatum dissociate amphetamine and apomorphine stereotypy: similarities to Huntingdon's chorea. Kainic acid lesion of cell bodies in the dorsal striatum enhanced the stereotypy-producing effects of d-amphetamine without affecting the sterotypy produced by the direct receptor agonist apomorph... | [
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PMID:26978 | Posterior spinal fusion with Harrington instrumentation using "balanced" anesthesia. | In our experience nitrous oxide-oxygen-morphine-curare anesthesia has proved preferable because of the need for swifly awakening patients after instrumentation to ascertain neurologic status. Adjunctively, doxapram has been used to awaken patients in a predictably short time span. To prevent massive blood loss, hypoten... | Posterior spinal fusion with Harrington instrumentation using "balanced" anesthesia. In our experience nitrous oxide-oxygen-morphine-curare anesthesia has proved preferable because of the need for swifly awakening patients after instrumentation to ascertain neurologic status. Adjunctively, doxapram has been used to awa... | [
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PMID:26980 | Filariasis in West Kalimantan (Borneo), Indonesia. | A survey was carried out among persons residing in 8 villages in the Province of West Kalimantan, Indonesia to determine the prevalence of filariasis. Finger tip blood smears were obtained at night from over 3,000 people and microfilariae of Brugia malayi were found in 108 (3.5%) and Wuchereria bancrofti in 10 (0.3%). ... | Filariasis in West Kalimantan (Borneo), Indonesia. A survey was carried out among persons residing in 8 villages in the Province of West Kalimantan, Indonesia to determine the prevalence of filariasis. Finger tip blood smears were obtained at night from over 3,000 people and microfilariae of Brugia malayi were found in... | [
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PMID:26982 | Cation and hydrogen ion effect on canine acid and gastrin output. | Differential stimulating effects on the parietal cell of dogs by calcium, magnesium and sodium were noted only at an alkaline pH. Acid inhibited the parietal cell, and antral perfusion did not cause gastrin stimulation at any pH level. The pH of the milieu of the parietal cell appears to be a more critical regulator of... | Cation and hydrogen ion effect on canine acid and gastrin output. Differential stimulating effects on the parietal cell of dogs by calcium, magnesium and sodium were noted only at an alkaline pH. Acid inhibited the parietal cell, and antral perfusion did not cause gastrin stimulation at any pH level. The pH of the mili... | [
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PMID:26983 | A new model for the study of septic shock. | Septic shock was produced in 28 healthy mongrel dogs by injecting 10(8) Escherichia coli organisms per kilogram into the gallbladder following division of the cystic artery and duct. Based upon the circulatory responses and the mortality, two distinct groups emerged. In one, the cardiac index decreased significantly, a... | A new model for the study of septic shock. Septic shock was produced in 28 healthy mongrel dogs by injecting 10(8) Escherichia coli organisms per kilogram into the gallbladder following division of the cystic artery and duct. Based upon the circulatory responses and the mortality, two distinct groups emerged. In one, t... | [
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PMID:26989 | Pus, deoxyribonucleic acid, and sputum viscosity. | On 100 sputum specimens selected from patients suffering from chronic bronchitis, bronchiectasis, asthma, and cystic fibrosis total deoxyribonucleic acid (DNA) content has been related to macroscopic type, to total dry weight yield, and to the apparent viscosity of the secretion at 1350 s-1: since DNA may be present, ... | Pus, deoxyribonucleic acid, and sputum viscosity. On 100 sputum specimens selected from patients suffering from chronic bronchitis, bronchiectasis, asthma, and cystic fibrosis total deoxyribonucleic acid (DNA) content has been related to macroscopic type, to total dry weight yield, and to the apparent viscosity of the... | [
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PMID:26992 | Androgen concentration and partial characterization of 5alpha reductase in the epididymis of the rhesus monkey. | The 5alpha reductase activity ofthe monkey epididymis was studied. The enzyme was found in particulate subcellular fractions, its distribution closely resembling that of the microsomal marker enzyme NADPH: cytochrome c reductase, suggesting an association of 5alpha reductase with membranes of the endoplasmic reticulum.... | Androgen concentration and partial characterization of 5alpha reductase in the epididymis of the rhesus monkey. The 5alpha reductase activity ofthe monkey epididymis was studied. The enzyme was found in particulate subcellular fractions, its distribution closely resembling that of the microsomal marker enzyme NADPH: cy... | [
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PMID:26993 | Study of complex marine sterol mixtures by mass-analyzed ion kinetic energy spectrometry. | Mass-analyzed ion kinetic energy spectrometry (MIKE) is shown to be an efficient and rapid method for the analysis of complex sterol mixtures. The method has been applied to the study of the free sterol fractions of five marine and one freshwater invertebrates. | Study of complex marine sterol mixtures by mass-analyzed ion kinetic energy spectrometry. Mass-analyzed ion kinetic energy spectrometry (MIKE) is shown to be an efficient and rapid method for the analysis of complex sterol mixtures. The method has been applied to the study of the free sterol fractions of five marine an... | [
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PMID:26994 | Influence of experimental cryptorchidism on cholesterol side-chain cleavage enzyme and delta5-3beta-hydroxysteroid dehydrogenase activities in rat testes. | Testicular cholesterol side-chain cleavage enzyme (CSCCE) and delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activities were assessed 12 hours and 2, 4, 8, 16, and 32 days after surgical induction of bilateral cryptorchidism in adult rats. Within 12 hours after surgery CSCCE activity (expressed as dpm of ... | Influence of experimental cryptorchidism on cholesterol side-chain cleavage enzyme and delta5-3beta-hydroxysteroid dehydrogenase activities in rat testes. Testicular cholesterol side-chain cleavage enzyme (CSCCE) and delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activities were assessed 12 hours and 2, 4... | [
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PMID:26995 | Lack of correlation between splenic and marrow hematopoiesis following irradiation or irradiation and transplantation in mice. | Differences were seen in the relative importance of spleen and marrow in early erythroid regeneration of lethally irradiated mice given spleen or marrow cell transplants compared with that in sublethally irradiated mice with surviving endogenous hematopoietic cells. Radioactive iron uptake was predominantly in the sple... | Lack of correlation between splenic and marrow hematopoiesis following irradiation or irradiation and transplantation in mice. Differences were seen in the relative importance of spleen and marrow in early erythroid regeneration of lethally irradiated mice given spleen or marrow cell transplants compared with that in s... | [
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PMID:26998 | Human red cell content of cyclic nucleotides and cations upon beta-adrenergic blockade. | The content of cyclic nucleotides (cAMP, cGMP) and Mg2+, Zn2+,Na+ and K+ was determined in human erythrocytes and after beta-adrenergic blockade in healthy individuals. The figures for cAMP and cGMP were 4.20 and 3.36 nmoles/ l intracellular volume, respectively. Only slight effects on the cyclic nucleotide content as... | Human red cell content of cyclic nucleotides and cations upon beta-adrenergic blockade. The content of cyclic nucleotides (cAMP, cGMP) and Mg2+, Zn2+,Na+ and K+ was determined in human erythrocytes and after beta-adrenergic blockade in healthy individuals. The figures for cAMP and cGMP were 4.20 and 3.36 nmoles/ l int... | [
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PMID:26999 | Denervation supersensitivity of the urethra to alpha-adrenergics in the chronic neurogenic bladder. | The response of the urethral pressure profile to the administration of various autonomic drugs was compared between a group of eight patients with chronic neurogenic bladder as evidenced by denervation supersensitivity to besacholineR and a group of 10 control subjects. A supersensitive response to the administration o... | Denervation supersensitivity of the urethra to alpha-adrenergics in the chronic neurogenic bladder. The response of the urethral pressure profile to the administration of various autonomic drugs was compared between a group of eight patients with chronic neurogenic bladder as evidenced by denervation supersensitivity t... | [
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PMID:26996 | [Content of adenine nucleotides and creatinephosphate in brain, myocardium, liver and skeletal muscle under combined action of hypercapnia, hypoxia and cooling]. | Cooling of rats under conditions of hypercapina and hypoxia induced no changes in the content of adenine nucleotides in the brain and skeletal muscles and decreased their concentration in the liver and myocardium. The content of creatine phosphate increased in the brain, but had no changes in the other tissues. 48 hour... | [Content of adenine nucleotides and creatinephosphate in brain, myocardium, liver and skeletal muscle under combined action of hypercapnia, hypoxia and cooling]. Cooling of rats under conditions of hypercapina and hypoxia induced no changes in the content of adenine nucleotides in the brain and skeletal muscles and dec... | [
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PMID:27000 | Uropharmacology: VII. Ganglionic stimulating and blocking agents. | A classification of the various ganglionic stimulants and blockers is presented, and their pharmacologic effects on the urinary bladder and urethra are discussed. Ganglionic stimulating drugs are of considerable interest in investigational work but are not presently used therapeutically. Ganglionic blockers include hex... | Uropharmacology: VII. Ganglionic stimulating and blocking agents. A classification of the various ganglionic stimulants and blockers is presented, and their pharmacologic effects on the urinary bladder and urethra are discussed. Ganglionic stimulating drugs are of considerable interest in investigational work but are n... | [
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PMID:26997 | [Immobilized form of Streptomyces griseus proteases complex]. | The immobilized form of proteases complex of Str. griseus actinomycete is obtained by means of glutaric aldehyde with aminoethyl cellulose. Hydrolysis of synthetic substrates showed that the system of peptide hydrolases is bound, but the ratio of activities as compared to the initial proteolytic complex changes, pH-opt... | [Immobilized form of Streptomyces griseus proteases complex]. The immobilized form of proteases complex of Str. griseus actinomycete is obtained by means of glutaric aldehyde with aminoethyl cellulose. Hydrolysis of synthetic substrates showed that the system of peptide hydrolases is bound, but the ratio of activities ... | [
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PMID:27001 | [Biochemical and hematological changes in the blood of horses after the "Velká Pardubická" steeple chase]. | Blood parameters were studied in two groups of horses in the "Velká Pardubická" steeple-chase in 1974, 1975 and 1976. After the race, the levels of lactate showed a manifold increase; an increase was also ascertained in the levels of glucose, sodium, potassium, haemoglobin, in the haematocrit value and in the number of... | [Biochemical and hematological changes in the blood of horses after the "Velká Pardubická" steeple chase]. Blood parameters were studied in two groups of horses in the "Velká Pardubická" steeple-chase in 1974, 1975 and 1976. After the race, the levels of lactate showed a manifold increase; an increase was also ascertai... | [
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PMID:27011 | Automated antibody detection in a low ionic strength (glycine) medium. | Modifications were introduced into the low ionic strength system of Lalezari. Glycine was added in place of acidified glucose and the sensitisation and aggregation phases were segregated. In the automated glycine system a stable baseline is readily obtained as strong cell aggregation, with risk of cell adhesion to coil... | Automated antibody detection in a low ionic strength (glycine) medium. Modifications were introduced into the low ionic strength system of Lalezari. Glycine was added in place of acidified glucose and the sensitisation and aggregation phases were segregated. In the automated glycine system a stable baseline is readily ... | [
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PMID:27006 | [Protein inhibitor of cyclic nucleotide phosphodiesterase in the retina in hereditary degeneration]. | Thermostable protein fraction from retina of rats with hereditary retinal dystrophy (Hunter and Campbell strains) did not inhibit cyclic nucleotide phosphodiesterase. At the same time an inhibitory component, found in retina of Wistar rat, rabbit, frog and lamprey, was similar to the component from bovine retina. Quant... | [Protein inhibitor of cyclic nucleotide phosphodiesterase in the retina in hereditary degeneration]. Thermostable protein fraction from retina of rats with hereditary retinal dystrophy (Hunter and Campbell strains) did not inhibit cyclic nucleotide phosphodiesterase. At the same time an inhibitory component, found in r... | [
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PMID:27010 | [Increase in tryptic digestibility of dietary proteins in the stomach]. | The trypsin assailability of food proteins (bread, dried milk and egg powder pastes) subjected for different periods of time to the action of the gastric juice directly in the stomach was studied by taking account of the specific topography of its content. It is shown that foodstuffs proteins can be increasingly assail... | [Increase in tryptic digestibility of dietary proteins in the stomach]. The trypsin assailability of food proteins (bread, dried milk and egg powder pastes) subjected for different periods of time to the action of the gastric juice directly in the stomach was studied by taking account of the specific topography of its ... | [
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PMID:27009 | [Dynamics and mechanism of mineral metabolic disorders in excessive phosphorus content in the diet]. | Keeping rats on a ration with a 1:3 proportion of calcium and phosphorus for 2 weeks results in hyperprosphatemia, hypocalcemia and renal calcinosis with uremia. When putting these animals on a food ration with the calcium and phosphorus ratio of 1:1 the concentration of these elements in the blood quickly returns back... | [Dynamics and mechanism of mineral metabolic disorders in excessive phosphorus content in the diet]. Keeping rats on a ration with a 1:3 proportion of calcium and phosphorus for 2 weeks results in hyperprosphatemia, hypocalcemia and renal calcinosis with uremia. When putting these animals on a food ration with the calc... | [
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PMID:27019 | Metabolism of atenolol in man. | 1. The disposition and metabolism of 1-(4-carbamoyl[14C]methylphenoxy)-3-isopropylaminopan-2-ol (atenolol, Tenormin) has been studied in man following oral and intravenous doses. 2. Approx. 50% of an oral dose was eliminated in urine; the major radiolabelled component was atenolol (approx. 90%). Faecal extracts also co... | Metabolism of atenolol in man. 1. The disposition and metabolism of 1-(4-carbamoyl[14C]methylphenoxy)-3-isopropylaminopan-2-ol (atenolol, Tenormin) has been studied in man following oral and intravenous doses. 2. Approx. 50% of an oral dose was eliminated in urine; the major radiolabelled component was atenolol (approx... | [
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PMID:27022 | On the evolution of an oligocephalic enzyme. glutamine-chorismate-amidotransferase-free anthranilate phosphoribosyltransferases from mutant strains of Salmonella typhimurium. | (1) A procedure has been described for the purification of two glutamine-chorismate-amidotransferase-free anthranilate phosphoribosyltransferases from mutant strains TAX6trpR782 and trpAB1653trpR782 of Salmonella typhimurium. (2) The native enzymes tend to aggregate forming polymers of molecular weights 333,000 in the ... | On the evolution of an oligocephalic enzyme. glutamine-chorismate-amidotransferase-free anthranilate phosphoribosyltransferases from mutant strains of Salmonella typhimurium. (1) A procedure has been described for the purification of two glutamine-chorismate-amidotransferase-free anthranilate phosphoribosyltransferases... | [
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PMID:27028 | [Analytical data of flunitrazepam (rohypnol), a new benzodiazepine-derivative, in special consideration of its metabolites (author's transl)]. | The article describes analytical data (TLC, GLC, IR, UV and MS) of flunitrazepam, 7-amino-flunitrazepam, desmethylflunitrazepam, 3-hydroxyflunitrazepam, 7-amino-desmethyl-flunitrazepam and many other benzodiazepine-derivatives, especially aminobenzophenones. Also extraction from biological specimen is reported. | [Analytical data of flunitrazepam (rohypnol), a new benzodiazepine-derivative, in special consideration of its metabolites (author's transl)]. The article describes analytical data (TLC, GLC, IR, UV and MS) of flunitrazepam, 7-amino-flunitrazepam, desmethylflunitrazepam, 3-hydroxyflunitrazepam, 7-amino-desmethyl-flunit... | [
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PMID:27046 | [On the distribution of myxobacteria in the temperate South America (author's transl)]. | 60 soil samples from Southern Chile, mainly from forests and grassland, yielded six species of myxobacteria. In forest soils Myxococcus coralloides and Myxococcus fulvus occur frequently, in grassland soils Myxococcus coralloides and Archangium gephyra are predominating. Myxococcus virescens, Melittangium lichenicola, ... | [On the distribution of myxobacteria in the temperate South America (author's transl)]. 60 soil samples from Southern Chile, mainly from forests and grassland, yielded six species of myxobacteria. In forest soils Myxococcus coralloides and Myxococcus fulvus occur frequently, in grassland soils Myxococcus coralloides an... | [
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PMID:27047 | [Neuraminidase activity of nonpathogenic Neisseria]. | It was revealed earlier by the authors that N. meningitidis of the principal serological groups A, B, and C possessed neuraminidase activity. In the present work it was shown that this sign was genus-specific, and that this property was also possessed by the representatives of nonpathogenic neisseria habituating in the... | [Neuraminidase activity of nonpathogenic Neisseria]. It was revealed earlier by the authors that N. meningitidis of the principal serological groups A, B, and C possessed neuraminidase activity. In the present work it was shown that this sign was genus-specific, and that this property was also possessed by the represen... | [
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PMID:27048 | [Study of hemosensitization characteristics for the purpose of standardizing erythrocyte flagellin diagnostica]. | The work is devoted to the study of conditions required for the purpose of creation of standard and the most active preparations of erythrocytic H-diagnostic agents. The dependence of binding of the H-antigen by erythrocytes on the pH and the ionic power of the medium was investigated. It was demonstrated that in the p... | [Study of hemosensitization characteristics for the purpose of standardizing erythrocyte flagellin diagnostica]. The work is devoted to the study of conditions required for the purpose of creation of standard and the most active preparations of erythrocytic H-diagnostic agents. The dependence of binding of the H-antige... | [
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PMID:27049 | [Method of culturing typhoid microorganisms with automatic regulation of glucose feed]. | A system of oxygen-glucose inverse relationship is not acceptable for cultivation of typhoid bacilli with a high reproductive rate and glucose utilization. The elaborated method of automatic glucose supply during typhoid bacilli cultivation permits to lead the process under the optimal conditions by pH and the residual... | [Method of culturing typhoid microorganisms with automatic regulation of glucose feed]. A system of oxygen-glucose inverse relationship is not acceptable for cultivation of typhoid bacilli with a high reproductive rate and glucose utilization. The elaborated method of automatic glucose supply during typhoid bacilli cul... | [
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PMID:27050 | [Acid-base balance during dosed fasting of schizophrenic patients with residual manifestations of organic lesions of the central nervous system]. | The indices of acid-alkaline blood balance was studied in the dynamics of dosed hungering in 29 patients with sluggish schizophrenia and 16 patients with residual signs oforganic lesions of the CNS. There were differences in the pH index, standard and buffer bicarbonates alkaline deficiency which indicated to different... | [Acid-base balance during dosed fasting of schizophrenic patients with residual manifestations of organic lesions of the central nervous system]. The indices of acid-alkaline blood balance was studied in the dynamics of dosed hungering in 29 patients with sluggish schizophrenia and 16 patients with residual signs oforg... | [
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PMID:27051 | Polymorphism of the cobalt-activated acylase in human tissues. | A method was elaborated for obtaining polyacrylamide gel zymograms of the cobalt-activated acylase after electrophoresis. Two fractions of the acylase showing activity towards N-chloroacetyl-gamma-L-glutamyl-beta-naphthylamide were found in human kidney, liver and intestine. The two fractions isolated from liver differ... | Polymorphism of the cobalt-activated acylase in human tissues. A method was elaborated for obtaining polyacrylamide gel zymograms of the cobalt-activated acylase after electrophoresis. Two fractions of the acylase showing activity towards N-chloroacetyl-gamma-L-glutamyl-beta-naphthylamide were found in human kidney, li... | [
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PMID:27052 | [Measurements and calculations on aglycon liberation from highly masked CMT selectines with beta-glucuronidase at pH 6. Realization of the principle of transport form-activity in anti-cancer drugs: theory of selectivity]. | The authors describe methods and results on the aglycon liberation from several glucuronides using beta-flucuronidase in vitro. The glucuronides examined are prototypes of new potent anticancer drugs (so-called CMT selectines). Basing on the knowledge of the values and parameters involved theoretical considerations res... | [Measurements and calculations on aglycon liberation from highly masked CMT selectines with beta-glucuronidase at pH 6. Realization of the principle of transport form-activity in anti-cancer drugs: theory of selectivity]. The authors describe methods and results on the aglycon liberation from several glucuronides using... | [
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PMID:27053 | [Effect of rapid changes of the oxygenation state and other external parameters on the long-term behavior of the energy metabolism in the erythrocyte]. | The red blood cell when transported through the circulatory system is exposed to a steady (approximately periodical) change of environmental conditions causing a permanent fluctuation of its important metabolic parameters. In a simple theoretical model it is demonstrated that a rapid oscillation of such parameters may ... | [Effect of rapid changes of the oxygenation state and other external parameters on the long-term behavior of the energy metabolism in the erythrocyte]. The red blood cell when transported through the circulatory system is exposed to a steady (approximately periodical) change of environmental conditions causing a perman... | [
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PMID:27056 | Reversibility of the blood-brain barrier dysfunction induced by acute hypertension. | An acute hypertensive episode of short duration (less than 3 min) induced by i.v. injection of angiotensin in rats resulted in a blood-brain barrier dysfunction that was rapidly reversible. Thus, no macroscopic extravasation was seen when Evans blue was given 10 or 20 min after angiotensin. A faint fluorescence (Evans ... | Reversibility of the blood-brain barrier dysfunction induced by acute hypertension. An acute hypertensive episode of short duration (less than 3 min) induced by i.v. injection of angiotensin in rats resulted in a blood-brain barrier dysfunction that was rapidly reversible. Thus, no macroscopic extravasation was seen wh... | [
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PMID:27055 | [Adult metachromatic leucodystrophy (author's transl)]. | A new adult case of metachromatic leucodystrophy (MLD) is reported. The earliest clinical signs are psychiatric in contra-distinction with the prevalent neurological signs observed in infantile or juvenile MLD. Although the occurrence of psychiatric features in adolescents or adults does not at first suggest MLD, it is... | [Adult metachromatic leucodystrophy (author's transl)]. A new adult case of metachromatic leucodystrophy (MLD) is reported. The earliest clinical signs are psychiatric in contra-distinction with the prevalent neurological signs observed in infantile or juvenile MLD. Although the occurrence of psychiatric features in ad... | [
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PMID:27060 | Measurement of cerebral blood flow in the fetal lamb with a note on the flow-distribution. | The cerebral blood flow was measured in the acutely exteriorized fetal lamb by 133Xenon washout and microsphere distribution techniques. The measurements were performed at different blood gas levels. Regional cerebral blood flow was calculated from the microsphere distribution for five different parts of the brain. Thi... | Measurement of cerebral blood flow in the fetal lamb with a note on the flow-distribution. The cerebral blood flow was measured in the acutely exteriorized fetal lamb by 133Xenon washout and microsphere distribution techniques. The measurements were performed at different blood gas levels. Regional cerebral blood flow ... | [
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PMID:27057 | Identification of beta-adrenoceptors and histamine receptors in the cat nasal vasculature. | The responses of the nasal capacitance vessels of the cat were recorded following intra-arterial injections of beta-adrenoceptor agonists and histamine H1-and H2-receptor agonists. Isoprenaline evoked vasodilation and propranolol (beta-adrenoceptor antagonist) caused a parallel shift to the right of the isoprenaline lo... | Identification of beta-adrenoceptors and histamine receptors in the cat nasal vasculature. The responses of the nasal capacitance vessels of the cat were recorded following intra-arterial injections of beta-adrenoceptor agonists and histamine H1-and H2-receptor agonists. Isoprenaline evoked vasodilation and propranolol... | [
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PMID:27061 | Alprenolol fails to antagonize the metabolic changes following repeated thyroxine injections in the rat. | Repeated injections of rat with 1-thyroxine (50 microgram/kg daily for 5 five-day weeks) retarded the weight gain of the animals and increased the absolute and relative size of the heart, adrenals and interscapular brown adipose tissue. In the myocardium and thigh muscle, thyroxine treatment resulted in elevated activi... | Alprenolol fails to antagonize the metabolic changes following repeated thyroxine injections in the rat. Repeated injections of rat with 1-thyroxine (50 microgram/kg daily for 5 five-day weeks) retarded the weight gain of the animals and increased the absolute and relative size of the heart, adrenals and interscapular ... | [
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PMID:27064 | Acute and long-term salt depletion and beta-blockade: plasma renin activity response and its relation to blood pressure reduction in long-term treatment. | The changes in plasma renin activity (PRA) during short-term salt depletion (and peroral furosemide on the first day) and after bolus injection of propranolol were compared to the change during long-term treatment with diuretic and with propranolol in 19 patients with benign primary hypertension. A highly significant c... | Acute and long-term salt depletion and beta-blockade: plasma renin activity response and its relation to blood pressure reduction in long-term treatment. The changes in plasma renin activity (PRA) during short-term salt depletion (and peroral furosemide on the first day) and after bolus injection of propranolol were co... | [
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PMID:27069 | Stereochemical studies of hydrogen incorporation from nucleotides with fatty acid synthetase from Brevibacterium ammoniagenes. | The biosynthesis of fatty acids from malonyl-CoA and acetyl-CoA was investigated with an enzyme preparation which was purified 100-fold from Brevibacterium ammoniagenes. Fatty acids synthesized in the presence of D2O and stereospecifically deuterated NADPH and NADH were isolated and analyzed by mass chromatography to e... | Stereochemical studies of hydrogen incorporation from nucleotides with fatty acid synthetase from Brevibacterium ammoniagenes. The biosynthesis of fatty acids from malonyl-CoA and acetyl-CoA was investigated with an enzyme preparation which was purified 100-fold from Brevibacterium ammoniagenes. Fatty acids synthesized... | [
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PMID:27072 | Studies on the function of the activator of sulphatase A. | The activator of sulphatase A is necessary for the enzymic degradation of sulphatides to cerebrosides at ionic concentrations in the physiological range (1). Activation is probably due to the reversible formation of a one-to-one complex between activator and sulphatides (1,2). Formation of this complex is partly inhibi... | Studies on the function of the activator of sulphatase A. The activator of sulphatase A is necessary for the enzymic degradation of sulphatides to cerebrosides at ionic concentrations in the physiological range (1). Activation is probably due to the reversible formation of a one-to-one complex between activator and sul... | [
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PMID:27085 | Renal consequences of narcotic abuse. | Heroin addiction is associated with several severe and occasionally fatal renal complications. Acute renal failure consequent to rhabdomyolysis and myoglobinuria, when treated supportively, carries a good prognosis. Staphylococcal or other bacterial septicemia may in itself prove fatal and is associated with a prolifer... | Renal consequences of narcotic abuse. Heroin addiction is associated with several severe and occasionally fatal renal complications. Acute renal failure consequent to rhabdomyolysis and myoglobinuria, when treated supportively, carries a good prognosis. Staphylococcal or other bacterial septicemia may in itself prove f... | [
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PMID:27086 | The effect of cyclophosphamide and methotrexate on the 'field effect' or unresponsiveness observed in the rat and mouse GvHR. | The Field effect is the decreased biological response to a second challenge in the graft versus host reaction (GvHR). Treatment of recipeint rats or mice during the initial GvHR by methotrexate or cyclophosphamide effected the secondary unresponsivienss. Methotrexate and cyclophosphamide demonstrated different temporal... | The effect of cyclophosphamide and methotrexate on the 'field effect' or unresponsiveness observed in the rat and mouse GvHR. The Field effect is the decreased biological response to a second challenge in the graft versus host reaction (GvHR). Treatment of recipeint rats or mice during the initial GvHR by methotrexate ... | [
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PMID:27087 | Conditions for the formation of cyclic compounds between pyridoxal phosphate and 5-hydroxytryptamine or 5-hydroxytryptophan. | 5-Hydroxytryptamine (5HT) and 5-hydroxytryptophan (5HTP) form cyclic compounds (probably of the tetrahydro-beta-carboline type) with pyridoxal phosphate (PLP). In the first step of reaction a Schiff's base is formed; during incubation it is transformed into a cyclic compound with a maximum absorption spectrum at 330 nm... | Conditions for the formation of cyclic compounds between pyridoxal phosphate and 5-hydroxytryptamine or 5-hydroxytryptophan. 5-Hydroxytryptamine (5HT) and 5-hydroxytryptophan (5HTP) form cyclic compounds (probably of the tetrahydro-beta-carboline type) with pyridoxal phosphate (PLP). In the first step of reaction a Sch... | [
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PMID:27090 | Effect of prior antibiotic therapy on concentrations of bacteria in CSF. | A prospective study was done to determine the effect of prior antibiotic therapy on concentrations of bacteria in CSF at the time of diagnosis. Concentrations of Haemophilus influenzae type b in CSF of partially treated patients were significantly smaller than in CSF of untreated patients. This was also true of patient... | Effect of prior antibiotic therapy on concentrations of bacteria in CSF. A prospective study was done to determine the effect of prior antibiotic therapy on concentrations of bacteria in CSF at the time of diagnosis. Concentrations of Haemophilus influenzae type b in CSF of partially treated patients were significantly... | [
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PMID:27091 | Effect of bile acids and pH on the release of enteropeptidase in man. | Bile acids increase the release of human enteropeptidase as well as other brush-border enzymes (alkaline phosphatase, leucine aminopeptidase) from duodenal mucosa, as had been shown earlier in experimental animals. The action of bile acids is independent of their known enhancing effect on enteropeptidase activity. The ... | Effect of bile acids and pH on the release of enteropeptidase in man. Bile acids increase the release of human enteropeptidase as well as other brush-border enzymes (alkaline phosphatase, leucine aminopeptidase) from duodenal mucosa, as had been shown earlier in experimental animals. The action of bile acids is indepen... | [
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PMID:27092 | The pediatric corner: the varied manifestations of Crohn's disease in children and adolescents. | Thirty children less than 15 years of age with Crohn's disease are reported. The mean age of onset was nine years and five months. Delay in diagnosis may stem from the varying modes of presentation. The mean delay in diagnosis was two years. Chronic diarrhea and/or recurrent abdominal pain associated with extraintestin... | The pediatric corner: the varied manifestations of Crohn's disease in children and adolescents. Thirty children less than 15 years of age with Crohn's disease are reported. The mean age of onset was nine years and five months. Delay in diagnosis may stem from the varying modes of presentation. The mean delay in diagnos... | [
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PMID:27093 | Effect of a new antacid on basal and meal-stimulated gastric secretion. | Our study of the comparative neutralizing effectiveness of four antacids on the acidity and pH of the basal gastric secretion in 32 patients showed that using the conventional doses of two tablets of Rolaids, two tablets of Di-Gel, 15 ml. of Maalox liquid or two tablets of Conquel, they are equal in their acid neutrali... | Effect of a new antacid on basal and meal-stimulated gastric secretion. Our study of the comparative neutralizing effectiveness of four antacids on the acidity and pH of the basal gastric secretion in 32 patients showed that using the conventional doses of two tablets of Rolaids, two tablets of Di-Gel, 15 ml. of Maalox... | [
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