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OTAR3088
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BioData-CellLine_annotated

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PMC11470999
E and F) Quantitative analysis of the signal intensity in the images from (C) and (D). (
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PMC11765988
From an immunopathogenic perspective, granuloma formation represents a pathological reaction initiated by CD4+ T cells interacting with antigen-presenting cells.
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PMC11489351
Figures 2C and S3A were created using sankeymatic.com and modified in Adobe Illustrator.
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PMC11585254
β-arrestins are recruited to GPCRs at the plasma membrane once they are phosphorylated on Ser and/or Thr residues within the cytoplasmic intracellular loops and/or the CT (Bünemann and Hosey, 1999; Luo et al., 2017).
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PMC9844987
Importantly, despite this association, the effect of identified features on promoter variability is still evident across a range of promoter expression levels (Figure 2F and G).
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PMC11694346
This stability suggests that BT-DNBS remained in a fluorescence-off state under these pH conditions.
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PMC11591038
BCCAO, bilateral common carotid artery occlusion; BNIP3, BCL2/adenovirus E1B 19-kDa protein-interacting protein; CCH, chronic cerebral hypoperfusion; HB-EGF, heparin-binding EGF-like growth factor; NTP, Neurotropin; OGD and OGD/R, oxygen–glucose deprivation and OGD with reoxygenation; PA-BSA, palmitic acid-bovine serum albumin; RNS, reactive nitrogen species; TI, thiamine insufficiency; ZDF and ZLC rats, Zucker diabetic fatty and Zucker lean control fatty rat.
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PMC11607764
The correlations between gene expression were assessed using the Pearson correlation coefficient.
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PMC11352877
Our results suggest that angiogenesis varies in the tested pathological conditions (ectopic endometriosis—12Z; ovarian endometrioid adenocarcinoma—A2780; tumors—SK-UT-1 and RL-95-2) compared to physiological angiogenesis (HME1).
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PMC9985266
Finally, the MEG3 promoter fragment was collected and purified for RT‒qPCR analysis.
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PMC8316344
E, F TRIB2 decreased labile iron in liver cancer cells.
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PMC11740399
Equal amounts of protein were subjected to 15% SDS-PAGE.
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PMC10988555
As expected, upon coordination, a deshielding of the Cp ring signal was observed compared to the neutral precursor [CoCp(CO)I2] (Δδ HCp = 0.61–0.77 ppm), mostly due to the change in the overall charge of the complexes.
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PMC6600253
Oxidative stress also participates in telomere erosion and DNA damage.
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PMC10831439
iNKT cells were isolated from peripheral blood mononuclear cells and transduced with an anti-CD19-CAR retrovirus.
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PMC11247842
3Manhattan plot for multi-trait meta-analysis by MTAG .
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PMC11360263
On the other hand, while progress has been made in the development of anti-HCMV drugs (such as ganciclovir, letermovir, cidofovir, maribavir, foscarnet, etc.)
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PMC8903741
For the purpose of developing a genetic construct for expressing a full-sized antibody in a CHO cell, we utilized a pcDNA3.1(−) vector, which is a broadly used mammalian expression vector.
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PMC11025866
NaB, the known inducer of the EBV lytic cycle, caused >100-fold upregulation of the EBV lytic gene BZLF1 in cells treated for 24 h, but not for 4 h or 6 h (Fig 2).
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PMC10073922
This strategy may be considered for the treatment of NSCLC.
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PMC9599726
We also confirmed by qPCR that TFRC mRNA was downregulated in VP-treated GIST882 cells.
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PMC11754436
The hFOB1.19 human osteoblast cell line (CL-0353), and the U2-OS (CL-0236) and MG63 (CL-0157) human OS cell lines were purchased from Procell Life Science & Technology Co., Ltd. The 143B human OS cell line was purchased from iCell Bioscience Inc, Shanghai iCell-h231).
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PMC11064533
In terms of future perspectives, first, proteomics data from this study point toward multiple hypothetical and putative proteins whose function and role require further investigation.
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PMC9429973
While in 2 cases the SCC was asymptomatic (11%).
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PMC6461034
As expected in the context of DNA damage signaling, INKA scores for ATM and PRKDC/DNA‐PK exhibited a time‐dependent increase after addition of neocarzinostatin (Fig 5E and F, and Appendix Fig S10).
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PMC9220170
This interaction with p97(VCP) is also necessary for the regulation of ER stress and degradation of ERAD substrates such as, for example, CD3δ .
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PMC11770130
The instrumental variables of plasma levels of ceramides were obtained from the metabolic syndrome in men (METSIM) study.
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PMC4277423
In a single-cycle assay to measure the infectivity of HIV-1 pseudotypes or wild type viruses including transmitted founder (T/F) viruses, 1×10 mock-, Cas9-HA-NLS and sgRNAs (CR1, CR2 or CR3)-transduced TZM.bl cells were transduced with HIV-1 pseudotype-containing supernatants equivalent to relative luciferase activity 200,000 to 500,000 or wild type viruses including transmitted founder (T/F) viruses at the MOI of 0.2 overnight.
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PMC11742047
OPLS-DA was carried out to identify the most important lipids in the separation between the treated and non-treated cells.
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PMC11626627
Western blot results indicated that after overexpressing circATP2B1, the expression of TWIST1, N-cadherin and vimentin decreased, while the expression of E-cadherin increased.
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PMC11224020
To test this hypothesis, we manipulated the shape of DCs in a controlled manner using a cell-confining device.
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PMC11786600
Kaempferol is a naturally occurring flavonoid recognized for its positive effects on human health, including anti-inflammatory, antioxidant, anti-diabetic, and atherosclerotic properties.22–24 Prior studies have demonstrated that kaempferol could reduce MMP9 activity and protein levels by modulating signaling pathways, facilitating macrophage phenotype polarization, and decreasing proinflammatory cytokine expression.25–27 Moreover, kaempferol inhibits the Mitogen-activated protein kinases (MAPKs)/ nuclear factor-κB (NF-κB) signaling pathway, suppresses the production of inflammatory factors such as IL-1β, and effectively alleviates the intensity of inflammatory response.28 Therefore, we used portable electrospinning technology loaded with kaempferol to design dressings that can be used for individualized and timely treatment of irregular wounds.
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PMC9429973
Methods: The 3D in-vitro model was developed starting from human decellularized femoral bone fragments, isolated from the trabecular zone, as structural 3D scaffold and biological ECM provider.
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PMC7582629
The reported Raman results provide information on cancer progression and allow the identification, classification, and follow-up after chemotherapy treatments of the cancer cells from the liquid biopsy.
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PMC11680982
This finding raises the intriguing question of whether neratinib potentially reverses resistance to CDK4/6 inhibitors combined with endocrine therapy, because of low HER2 expression in HR/HER2-low breast cancer.
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PMC11351758
Mitochondrial damage is a healthcare concern that can be found in a number of diseases, including Alzheimer’s disease and liver and heart conditions, and it is also an observed phenomenon usually associated with hair loss, osteoporosis, and aging .
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PMC11267830
A subsequent clinical trial revealed that the response rate for patients with heavily pretreated AML treated with indirubin combined with idarubicin and cytarabine was 35% .
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PMC10669128
However, to generate and maintain GEM mice is expensive which, added to the complexity of results interpretation, leads to critical limitations .
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PMC11469524
In here we use the term ‘coefficients’ for both, if identifiable, and if not the structurally identifiable combinations thereof will be termed ‘coefficient paths’.
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PMC10237474
no. 12338018, Thermo Fisher Scientific).
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PMC11754436
Cell proliferation analysis via Cell Counting Kit-8 (CCK-8) assays.
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PMC11402217
The Model group, receiving saline, began to show mortality from day 15, with no survivors by the end of the treatment period.
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PMC10890055
This standard was originally developed for non-degradable materials and, therefore, in case of degradable materials, often leads to exaggerated, i.e., more toxic, responses than expected in vivo.
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PMC11310713
HRT was initiated at approximately 4 to 5 weeks after OVX when ovarian hormone levels reached a baseline .
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PMC3765139
In these conditions, the D10 cell line - as well as the Na8 and HBL cell line - were clearly generating spheroids (Figure 2A-C).
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PMC11471176
IC50 was about 265 mM for MDA-MB-231.
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PMC11683240
In the following experiments, we used the plasma-derived α1AT, available as approved drug (Prolastin), to unravel the underlying mechanism of PT inhibition.
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PMC8873393
Next, we divided 11,764 expressed RefSeq genes (with FPKM ≥ 1 in at least one fraction) into seven clusters based on their transcript expression profiles in the two subcellular fractions and four time points (Fig. 3A and Table S2).
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PMC10809689
A significant difference in GDH mRNA expression at 48 and 72 h of treatment with Gal-9 in comparison to the PMA group was observed (p = 0.0001) (Fig. 3 (c., d.)).
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PMC11772585
RNA-seq analyses were conducted by OE Biotech (Shanghai, China).
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PMC11806106
G Averaged BRET-based measurement of HEK293 cells stably expressing the KOR BRET-sensor.
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PMC8658661
Such inaccuracies in wording can affect the design of studies and the interpretation of experiments designed and performed to answer the biomedical question.
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PMC9243326
The signal transduction (4,297, 16.33%) category had the largest number of unigenes.
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PMC10840195
Determination of the proliferation ability of SR-A498 cells overexpressing Twist by CCK-8 assay (A).
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PMC11785489
Including AAIS as a covariate did not substantially change the estimated effect size of TCR-mem (conditional βTCR-mem = 0.14, 95% CI = [0.12–0.15], heterogeneity p = 0.92).
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PMC8427838
Our previous analysis of data sets from The Cancer Genome Atlas and our own immunohistochemical analysis revealed that low expression of TMIGD1 correlates with poor survival in renal and colon cancers .
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PMC10671321
As expected, the mutated ColQ has a higher molecular weight compared to the WT ColQ in agreement with the sequences (see images of western blots in Figure 2C,E), indicating that the mutated peptide is not cleaved.
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PMC11612315
The mice were anesthetized using isoflurane, and blood samples were collected at specified time points.
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PMC10237474
The separation was performed on a 2.1 mm x 75 mm C4 reverse phase column (Mac-mod) with a gradient of 5–95% B (solvent A, 0.1% formic acid in water; solvent B, 0.1% formic acid in acetonitrile) at a flow rate of 0.75 mL/min for 12 min.
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PMC9874064
Scale bar ​= ​200 ​μm.
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PMC2193049
As expected, addition of a saturating amount of the corresponding MAGE-3271–279 synthetic peptide to mock-transfected cells resulted in a strong production of TNF-α.
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PMC11783017
We found that treatment with Cis/Mn/NH@PGP significantly enhanced the induction effect of ICD in tumors.
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PMC11694066
B, Assessment of replication fork speed (kb/minute) in JN1 cells exposed to DMSO control, or a combination of PARPi talazoparib (Tala) and ATRi M4344 for 6 hours, in the presence or absence of siRNA-mediated silencing of EWS–WT1.
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PMC11609529
Western blotting confirmed the near absence of MAML2 protein in MAML2-KO ES-2 cells (Figure 5A).
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PMC7504302
Of particular interest, the authors of this study postulated that the two malignancies had distinct epimutation mechanisms, mainly induced by asbestos vs. smoking.
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PMC11400680
The second group of cell segmentation methods is instance segmentation.
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PMC10421378
These compounds can enhance probiotic adhesion to Caco-2 cells by modifying the cell surface characteristics of both the probiotics and the Caco-2 cells.
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PMC11731614
KDEVs from SEC Fr2/3 are functionally internalized by primary DRG neurons.
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PMC11012012
In the study of Bund et al. (2006), 18 out of 18 CLL patient samples were SLAMF3/CD229-positive by flow cytometry, with a median of 63% positive CLL B cells .
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PMC10652261
Mp 117–118 °C.
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PMC11190538
C-PC was able to scavenge DPPH free radicals by antioxidant activity up to 99.12 ± 0.027 % compared to the reference standard (Table 3).
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PMC9688728
The analysis of trajectories of metabolite abundances revealed a series of characteristic changes in the content of groups of low-molecular-weight substances over time.
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PMC11727145
To assess cell viability on the DED constructs, a 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide conversion assay (MTT; Thermo Fisher Scientific, Waltham, MA, USA) was performed in parallel on DED samples.
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PMC11767725
Adipose tissue expansion leads to hypoxia, triggering macrophage infiltration.
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PMC11306019
Additionally, we observed that the hydroxyl group at C-3 of the C-ring significantly influences the antagonistic effects against BTZ.
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PMC9429973
Median time to first response across dosing cohorts was 0.95 months (range 0.9-9.7); median duration of response was not reached.
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PMC8348645
The percentage analysis of the viable, early, late and necrotic cells as and the concentration increases were calculated, showing that the sample W. somnifera leaves treated at 80 µg/mL and 160 µg/mL induced early and late apoptosis in the HepG2 cells, with 8.39%, 11.33% of early apoptotic cells and 17.82%, 19.91% late apoptotic cells, respectively.
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PMC9429973
F. lin, T. han, Y. zhang, Y. cheng, Z. xu, X. mo, F. wang, C. yan, Y. sun, J. wang, F. tang, W. han, Y. chen, Y. wang, X. zhang, K. liu, X. huang, L. xu Peking university institute of hematology, Beijing, China Background: Secondary poor graft function (sPGF) increases the risk of life-threatening complications after transplantation and is an obstacle to achieving better outcomes after hematopoietic stem cell transplantation (HSCT).
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PMC10818573
We used a reaction temperature of 39 °C and a reaction time of 15 min.
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PMC6684588
Scale bar, 100 µm. (
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PMC10158546
Strikingly, these ReN cell-derived cultures were incapable of replicating multiple prion isolates despite the high level of PrP expression.
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PMC11441402
A stock solution of 500 µM was prepared in dimethyl sulfoxide (DMSO, Fisher Bioreagents, USA) and all subsequently prepared concentrations contained 0.2% DMSO.
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PMC10728535
Boxplot of ovarian cancer sample arrays (A) before and (B) after batch correction.
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PMC11683240
Cells were left untreated as control (Con).
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PMC9429973
Secondary endpoints include overall survival (OS), overall response rate (ORR), duration of response (DoR), safety and tolerability, and patient reported outcomes.
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PMC11116779
Research on the respiratory toxicity of MPs/NPs remains significantly limited (20, 21, 23).
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PMC2196094
One possibility, which we presently can not exclude is that rat MMCs also express RMCP-5.
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PMC9429973
Eligible DLBCL patients who had HIF-1α tissue expression ≥10% were included.
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PMC5941560
Table 2Jurkat variants found in the ClinVar databasersIDJurkat AFGenePhenotypeClinVar accessionClinVar substitutions rs63750636 1.0 MSH2 Lynch syndromeRCV000076405.3 rs397517342 0.75 CDH23 Usher syndrome type 1DRCV000039224.2 rs397516435 0.25 TP53 Li-Fraumeni syndromeRCV000205265.3ClinVar short deletions rs63750075 — MSH6 Lynch syndromeRCV000074711.2 rs398122841 — BAX Carcinoma of colonRCV000010120.5 rs397508104 — KCNQ1-(AS1) Long QT syndromeRCV000046039.3 rs750664956 — ASPM Not providedRCV000217980.1 rs786204835 — PURA Not providedRCV000169739.5ClinVar short insertions rs397507178 — RAD50 Hereditary cancerRCV000030958.3 rs398122840 — BAX Carcinoma of colonRCV000010119.5 Jurkat variants found in the ClinVar database Moving from established to predicted effects, we used SnpEff to predict the functional consequences of the GATK-called small variants.
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PMC7189327
The convincing evidence has suggested that gender, age, sunlight exposure (especially ultraviolet radiation), smoking, chemicals, and fair skin were all BCC risk factors .
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PMC8806312
The RNAseq data and corresponding clinical follow-up information were downloaded from the public TARGET database, which included a total of sixty-five OS nonmetastatic samples and a total of twenty-two OS metastatic samples.
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PMC9429973
Mortality was highest in wave 1 (March – June 2020), decreasing in wave 2 (Sept 20 – March 2021) and again in wave 3 (May 2021 – current), with 4-week mortality figures of 44%, 26% and 8% respectively.
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PMC11666785
The cells were transfected to express photoactivable protein (Dendra2-Actin/Dendra2-Tubulin/mEos-Tom20) that are targeted to the specific organelle (such as, actin filaments, tubulin and mitochondrial network).
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PMC9429973
Of these patients, 7 (53.8%) received imatinib (6 with 200 mg doses and 1 with 100 mg), 1 (7.7%) received bosutinib 100 mg and 5 (38.5%) received dasatinib 20 mg.
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PMC11247842
In addition, a copy number variant (CNV) in GC has been proposed as the potential causal mutation .
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PMC11786156
WST-1 assay was performed with HBL-2 and recombinant human LFA1.
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PMC11684269
Given the tissue heterogeneity, the kidney TRM cells are not entirely composed of CD8 T cell subsets but also possess CD4 T cell subsets.
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PMC11761919
seral AST. (
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PMC11665584
A smaller proportion of HF keratinocytes, compared with the healthy skin scRNA-Seq data set, was observed in both conditions (51.2% control wound, 60.7% PITX1 wound versus 72%/75% in healthy tissue) (Supplemental Figure 4C).
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PMC3203927
As is the case in box-plot data presentations, the lowest bar represents the observed sample minimum, the base of the box represents the lower quartile while the top represents the upper quartile, the bar inside the box represents the median, and the top bar represents the highest sample observation.
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