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PMC11087055
White asterisks, the polonaise movements at the induced axis which are opposite direction to the authentic axis.
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PMC7757104
We hypothesize that upregulation of OPNc may contribute to the increased expression of the cytokines analyzed in the present study through the activation of their corresponding cellular receptors.
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PMC9429973
Our data support that application of additional immunohistochemical studies are recommended to identify underrecognized SM when KIT mutations are unexpectedly found by molecular assays.
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PMC11298021
The expression of miR-3143, miR-3182 and miR-501-3p showed a significant decrease in comparison with healthy normal samples by about a thousand-fold (****P<0.0001).
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PMC8345486
The 48 h treatment with cisplatin or compound 23 alone reduced cell viability only in A2780 spheroids, as described above.
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PMC9856122
An anti-HIV-1 and HIV-2 reverse transcriptase (RT) study included fangronine chloride and nitidine chloride to determine the antiviral potential.
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PMC8623816
The nucleotide sequence and genomic organization of the three novel mycoviruses, Rhizoctonia solani endornavirus 1 (RsEV1), Rhizoctonia solani dsRNA virus 1 (RsRV1) and Rhizoctonia solani partitivirus 5 (RsPV5), were also reported by our laboratory .
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PMC9429973
Methods: This prospective single-center study measured the severity of anxiety in an unselected cohort of subjects referred to a hematologist for the first time using a internationally validated anxiety-focused questionnaire, Generalized Anxiety Disorder-7 (GAD-7).
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PMC11753435
Binary accuracy is the accuracy of the model in predicting the correct binding pairs when given two protein-peptide pairs.
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PMC11731614
The differentially expressed REACTOME pathway with ‘MAPK Family Signaling Cascades’ included both Mapk1 and Mapk3 (Fig. 3G left; adjusted p-value ≤ 0.05), with Mapk1 found in fraction 2/3 (Fig. 3F).
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PMC4270159
b. Harvest protein as in Step 5 after 2 hr of treatment.
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PMC11200978
While various options exist, many, if not all, remain suboptimal.
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PMC7351993
Note that for avoiding aggregation of the particles, 0.5%-Tween 20 was added to the sample solution.
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PMC9429973
4 line, 7 pts (11,4), 1 pts were TPH.
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PMC10495912
Comparisons between groups were analyzed, by the SPSS software version 18, using the one-way ANOVA with Tukey’s test.
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PMC8592717
Similarly, PAL also showed anti-tumor activity by hindering the proliferation of MCF-7 human breast cancer cells through activation of DPPH radical-scavenging activity .
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PMC11394730
Here, they are converted into conjugates of quercetin .
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PMC9118379
In contrast, using the CMSe approach yielded 282 variant PSMs passing a gFDR of 1% that corresponded to 93 variant peptides with 82 variant events, more than using the SSe approach.
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PMC9429973
W. Boufrikha, R. Mezriqui, N. Slama, S. Chouchene, S. Abidi, Z. Mlayah, A. Benabdeljelil, M. Ftita Hematology; Hematology Laboratory, Fattouma Bourguiba University Hospital, Monastir, Tunisia Background: Several studies described numerous factors to affect the prognosis and predict the severity of COVID-19.
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PMC10490530
BLI was performed 30 min after cell implantation, and sequential image acquisition was carried out between 5–15 min after intraperitoneal injection of 3OH-hispidin (3 mg in 100 μL sterile PBS).
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PMC9100622
Raw cell-count values were obtained by the average of 4 image fields per well and transformed in log function to obtain a robust Z-score-standardized robust estimator value, as previously described .
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PMC11659921
Additionally, we assessed the predictive capability of this model for 3‐year, 4‐year, and 5‐year survival outcomes of OV patients, yielding AUC values of 0.642, 0.652, and 0.659, respectively.
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PMC11792888
Each bar represents mean ± SE (n = 3). (
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PMC9429973
Summary/Conclusion: Similar PK and PD results were observed in patients treated with IBER monotherapy, Iber+d, and IberDd.
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PMC11585565
Pathogenic variants from JS patients are indicated in bold text, with those included in this functional study highlighted in bold red text (created with BioRender.com). (
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PMC2944824
To determine cytochrome C release, cytosolic fraction were subjected to Western blotting and probed with a rabbit antibody against cytochrome C and subsequent goat anti rabbit antibody with an infrared probe (D).
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PMC6742971
The sensitivity of a T cell to antigen is influenced by multiple factors: the affinity of the TCR-peptide-MHC interaction, and the engagement of multiple other receptors on T cells and the density of these receptors on the T cell surface within the T cell synapse (4, 26, 27).
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PMC11705901
One recent study reported the A218T variant of the OXTR that results from SNP rs4686302 altered intracellular signaling compared to WT OXTRs when expressed in HEK293 cells .
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PMC11700523
COS-7 cells were routinely maintained in Dulbecco’s modified eagle medium (DMEM, FUJIFILM, Tokyo, Japan) supplemented with 10% fetal bovine serum (Funakoshi Co. Ltd. Tokyo, Japan) and 1% penicillin/streptomycin (FUJIFILM).
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PMC11674834
CHO cells transfected with NAT1*14B showed lower 3,4-DMA KM values than NAT1*4, reflecting its higher affinity towards 3,4-DMA.
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PMC9429973
Although, this data can be influenced by an accurate hematological follow-up, we can’t exclude a real increased rate of MGUS in MPN patient.
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PMC11532793
This matches the biological variability and heterogeneity that we typically observe our own in-house RNA-seq experiments using genetically identical laboratory mice (6).
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PMC11026382
TYMS R166Q is an active site mutation with near complete loss of catalytic activity.
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PMC11354225
After 24 h, cells were incubated with EGCG 10, 20 and 40 µM prepared in DMEM with FBS and without phenol red for 4 h in darkness at 37 °C with 5% CO2.
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PMC11764090
BRs actively control cold stress response by promoting expression and cold tolerance of CBF1 and CBF-regulated gene COR47 in A. thaliana .
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PMC9429973
MSCs were cultured by standard methods.
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PMC11759543
Jin-Yi Wu: Methodology.
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PMC11295144
The images were then processed by the Structured Illumination and Channel Alignment function in Zen 2012 Software.
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PMC9429973
Summary/Conclusion: Our study showed significant difference in the characteristics and outcome in covid-19 between patients with LPD and patients from GP.
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PMC7757104
Indeed, the mRNA expression levels of the GP130 receptor were downregulated in the OPNc-knockdown cells in the present study.
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PMC11637276
b, e, h) Overall survival of patients with PDAC with high (red) or low (black) α2 integrin (ITGA2, b), β1 integrin (ITGB1, e) and MAP3K1 (h) expression. (
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PMC10854447
H NMR (400 MHz, δ ppm DMSO-d6): 8.81 (s, 1H, triazole-CH), 7.99 (d, J = 7.9 Hz, 2H, Ar–H), 7.90 (d, J = 8.5 Hz, 2H, Ar–H), 7.62–7.56 (m, 3H, Ar–H), 7.48 (t, J = 7.6 Hz, 2H, Ar–H), 7.37 (t, J = 7.4 Hz, 1H, Ar–H), 6.29 (s, 2H, N–CH2).
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PMC11743975
Histogram of ATP production in control COS-7 cells with or without niclosamide treatment and in COS-7 cells expressing either Tom20-CIBN-GFP or with Opto-MitoA module, exposed with both niclosamide and light.
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PMC9429973
Our results demonstrate the long-term benefit of A-based regimens in CLL pts with higher-risk genomic features, regardless of line of therapy.
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PMC10213199
This sample number ensured sufficiency of samples, given the substantial mortality rate at all stages.
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PMC11530949
This elevation in ROS and NO levels is critical in inducing oxidative stress and promoting apoptosis in cancer cells.
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PMC9429973
The median age was 2 years (9 months to 18 years).
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PMC9727330
Importantly, our findings proved that NNDAsp targets ASNS enzyme indicated by ELISA assay and flow cytometric analysis in the Caco-2 cell line.
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PMC3888431
The Trinity Assembler performed best in this respect with 38% correct and 14% incomplete transcripts (Figure 4).
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PMC11049548
When it comes to the intracellular levels of niacinamide, glutamate, proline, asparagine, and creatine, the HGSOC cells are quite different from both sensitive and resistant A2780 cells, which instead are relatively similar to one another; the first three metabolites result in significantly increased in HGSOC cells, while the other two are significantly decreased (Figure 5).
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PMC11424574
Experimental procedures followed the Guidelines of Animal Use and Care of the Chung Shan Medical University.
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PMC4005030
In the lipid peroxidation assay 3, 4-dihydroxyphenylacetic acid was observed to be the most effective compound.
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PMC7039683
GR: GRHL, TE: TEAD, FO: FOS, TF: TFAP2, GA: GATA, CE: CEBP, KL: KLF. (
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PMC2944824
The mechanism appears to involve blunting p53 mediated apoptosis by down-regulating p53 expression.
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PMC11525028
The Bliss scores were used to assess the synergistic effects of inhibitors.
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PMC11762280
B Photos of mice dorsal skin after different UVB irradiation doses and times (n = 4).
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PMC11806182
MiRNA-target network highlighted key genes such as PHF8, LCOR, SFT2D3, E2F1, and ESR1, which were found to be prognostic for patient survival.
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PMC11502327
Studies have shown that the natural dietary compound Kaempferol exerts anti-inflammatory effects by down-regulating the NF-κB pathway, inhibiting interleukin-4 and cyclo-oxygenase 2 expression (200).
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PMC10747139
Swaminatan et al. (2022) reported that RAED-C is highly active in primary tumors, whereas RAPTA-C is inactive in primary tumors but possesses antimetastatic and antiangiogenic properties.
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PMC11279397
The results showed that main terpenoids met the criteria of Ro5 and the value of TPSA ≤ 140 Å and represented promising or moderate ion channel modulator and enzyme inhibitor affinity.
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PMC9429973
We have interrorgated key transcription factor nodes with RNAi Methods: Primary cells were co-cultured on primary hMSCs for perturbation experiments.
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PMC9429973
Aims: To further characterize the erythropoyesis of these patients and to perform lentiviral transduction of the WT gene to observe the rescue of the phenotype observed in the patients (multinucleation, macrocytosis...).
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PMC8540692
As presented in the Figure 3, the analysis of the combinatorial effect of the compounds on cell viability revealed that CCM and NaBu synergistically reduce the viability of GBM cells when the concentrations used are higher than 1 µM CCM and 5 mM NaBu.
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PMC11247842
All contributors used the same reference genome for imputation, i.e. ARS-UCD1.2 and the WGS reference from the 1000 bull genome project (BGP) Run 7 .
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PMC11755519
This effect on angiogenesis is crucial for cancer cells, as they require oxygen and nutrients for survival and proliferation.
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PMC11190062
TLR4 and TLR9 activation inhibited the efficacy of proteasome inhibitors bortezomib and carfilzomib, drugs used in the treatment of MM.
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PMC7757104
The cytotoxicity assays performed in the present study revealed that ACRP cells became more sensitive to CDDP compared with A2780 cells when CDDP concentrations were progressively increased (data not shown).
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PMC11188874
B, Effect of HDAC6 inhibitors on ChT-like proteasome activity in CD138 myeloma patient cells.
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PMC9429973
With a median follow-up of 55 months (range, 3-119), the 5-year continuous remission and OS rates were 76% and 47%, respectively (Figure 1B).
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PMC9429973
Results were correlated with clinical features such as Dynamic International Prognostic Scoring System (DIPSS) classification, splenomegaly, hemoglobin level and other negative prognostic factors.
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PMC9681981
SEM: Standard error mean Interaction of cell treatments and time on α-toxin gene expression (P<0.05) The means with dissimilar letters have significant differences based on Duncan’s multiple range tests and the means with the same letter are not significantly different.
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PMC6034753
Observations on the inhibition of apoptosis function by Meq have corroborated the functional significance of the interactions of these proteins, with potential roles in MDV and CAV pathogenesis in co-infected chickens.
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PMC9429973
Aims: In this study, we aimed to compare the engraftment kinetics and long-term survival data after autologous stem cell transplantation in lymphoma patients who underwent stem cell mobilization with plerixafor.
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PMC11143482
This study managed to establish a humanised xenograft animal model of OS metastasis to the lung.
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PMC11767725
TNF-α induces serine phosphorylation of IRS-1, impairing insulin receptor signaling.
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PMC11684821
Classically, CTL express the CD8 glycoprotein and recognize processed proteinaceous antigens as short peptides of 8–14 amino acids in length presented by major histocompatibility complex class I (MHC-I) molecules (1).
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PMC11525028
Scale bar: 100 μm (n = 3). (
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PMC11743975
After another 30 min of recovery in the dark, we observed an increase of dispersed mCherry fluorescence in the cytosol, which indicates the reversibility of the CRY2clust and CRY2-CIBN modules.
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PMC11352746
This may be the reason why some of the publications reviewed resorted to the conventional MTT, Cell Titer-Glo, and Ethidium homodimer assays instead of using the fluorescence component of the IncuCyte.
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PMC5874706
In all experiments THN7 was used at a final concentration of 6 μM. A427 cells were selected as a model because they have been reported to overexpress CK2α, the target of kinase inhibitors from the class of indeno[1,2-b]indoles .
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PMC11087055
These results suggest that PS morphogenesis is not responsible for the initiation and early phase of the polonaise movements.
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PMC9429973
In order to better define TP53-dependent and independent pathways linked to these phenotypes, LT-HSC, ST-HSC, MPP, CMP, MEP and GMP from normal, JAK2V617F and JAK2V617F/TP53 KO mice were cell sorted and RNA-Seq analysis was performed.
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PMC11591794
The GA/DOX combination showed the highest level of P53.
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PMC10443665
Tragacanthin could be considered a potent cytotoxic agent affecting both MTX-sensitive and -resistant CCRF-CEM cell viability.
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PMC6461034
Leave for 10 min at room temperature and mix gently every 2–3 min.
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PMC11243198
Further, they showed that some of the derivatives they identified could be docked at ZF4, while others also had an alternative best-scoring docking pose with the putative GANT61 binding site.
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PMC9577200
When the BC and PAO1 were exposed to CS concentration at 0.2 %, the lowest viability was 73.3 and 70%, respectively, but when the CS concentration was 1.5%, the minimum viability for BC and PAO1 was 99 and 94.33%, respectively (Figure 5A).
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PMC11683240
B–D, PTS1 (84 nM) and different concentrations of α1AT or the respective amount of solvent (H2O) (Con) were added directly to CHO-K1 cell lysate and biotin-NAD and incubated for 40 min at room temperature.
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PMC5417661
A study of the maximal tolerated dose of the NOV202 in NMRI nu/nu female mice was performed before the xenograft study.
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PMC10502403
To determine the mechanisms underlying these promising effects, we investigated metabolic characteristics.
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PMC11387401
Overall, there was only a limited knockdown observed for AFF1 ASO1 and good knockdown for H4C9 ASO1, 20 and 80% knockdown, respectively.
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PMC11791264
While SETD7 KD significantly impaired cell proliferation and migration (Figs. 1, B–F and S1A), SETD7 OE promoted these processes (Figs. 1, G–J and S1B).
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PMC11407042
Transfer cells to two or more dishes if necessary.j.
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PMC6024716
Cell viability and luciferase activity were determined using the CellTiter-Glo luminescent cell viability assay and the One-Glo luciferase assay system (Promega cat# G7570 and cat# E6110, Madison, WI, USA, respectively), following manufacturer’s protocol.
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PMC11320834
All lipids were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA).
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PMC11697189
A, B) The top 20 mutated genes in the high-risk group (A) and low-risk group (B). (
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PMC11792766
No. A38800), 2‐aminofluorene (2AF) (Sigma Aldrich, Cat.
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PMC9847912
We also determined the toxicity of GSK232 in CEMss and MAGIC-5A cells in order to preclude the involvement of inhibitor-induced cytotoxic effects in any observed antiviral responses.
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PMC11621565
These results suggest that the balance between the pro-inflammatory LPS and the anti-inflammatory SCFA in the gastrointestinal tract is disturbed in PD patients.
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PMC9429973
Meaningful PD activity and clinical efficacy were observed at 50 and 75 mg doses.
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