PMCID
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PMC11240052
Circular RNA 0051240 promotes cell proliferation, migration, and invasion in ovarian cancer through the miR-637/KLK4 axis (20).
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PMC11323699
In the first one, mice were treated one day after tumor inoculation with olaparib for 2 weeks followed by 4 weeks of TAX2.
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PMC6901583
a The indicated cytokines expression in GIST882 cells stably expressing shcon or shBRD4 were analyzed by RT-PCR.
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PMC11786767
Images at different mitotic stages were acquired using an LSM980 confocal microscope with Airyscan2.
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PMC8735881
As shown in Figure 3(D-F), the TZM-bl cells expressing GPI-scFvs also displayed different resistance to the transmission of the three tested viruses from CEMss-CCR5 cells, and in comparison, GPI-10E8 was still a more effective inhibitor than other constructs in terms of the breadth and potency.
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PMC11758416
Figure 2Confirmation of CD32 expression and example gating strategy for L cell death measurement(A) L cells expressing CD32 and control L cells were stained with an anti-CD32 antibody and assessed by flow cytometry.(B) Gating strategy for Annexin V and 7-AAD in a non-stimulated effector T cells sample.
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PMC11361748
Cells were treated with TPA for 30 min and immunostained for PKCα and phospho-PKCα (S657), and for nuclei (DAPI).
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PMC11609529
These findings indicate that depleting both YM and MAML2 reduces cell proliferation and enhances apoptosis.
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PMC9429973
Altered transcription may occur at three-dimensional chromatin level and be orchestrated by a combination of multiple regulatory factors.
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PMC11806106
For the NOP FRET sensor the following primers were used: vector+mTurq fwd: TCTCAGACAGAGTGAGGTCAACCGGTGTGAGCAAGGG; vector+mTurq rev: GCCGGAAACAGGGGCTCCATGGTGAATTCCACCACACTGGA; fragment 1 fwd: CCAGTGTGGTGGAATTCACCATGGAGCCCCTGTTTCCG; fragment 1 rev: CCCTTGCTCACCATCCGCGGTCCACTCAGCAGTCTAACGCCTCG; YFP fwd: GTTAGACTGCTGAGTGGACCGCGGATGGTGAGCAAGGGCGAG; YFP rev: GGTCCTTCTCCCTGCTGTTAACCTTGTACAGCTCGTCCATGCC; fragment 2 fwd: GGACGAGCTGTACAAGGTTAACAGCAGGGAGAAGGACCGC; fragment 2 rev: CTCGCCCTTGCTCACACCGGTTGACCTCACTCTGTCTGAGACTTGTACA.
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PMC11726183
The MDS analysis was performed for 100 ns time period comprising 55 414 total atoms including water molecules.
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PMC9429973
ELN stratification is based on genetic abnormalities and divides AML in low, intermediate and high risk groups.
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PMC6450504
Briefly, HeLa cells were seeded on glass coverslips in 12-well plates at 100,000 cells/well one day before treatment.
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PMC11757131
The effects of different concentrations of 3-HIB on cell proliferation were tested after 2, 4, 8, 16, 24, and 48 h (Table 1).
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PMC11291490
The reaction was halted by adding 2 M H2SO4 (50 μL/well), and the OD450 was measured using a spectrophotometer (Bio-Rad, USA).
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PMC11786600
These experiments adhered to the guidelines established by the National Institutes of Health concerning the care and use of experimental animals and complying with international ethical standards.
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PMC9341513
In the long term, it may then be possible to predict changes to expression using only input data from healthy cells, plus the coordinates of the rearrangement.
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PMC11627060
Primary rabbit polyclonal antibodies (Bcl-2, Bax, and Caspase-3) and secondary antibodies were obtained from Abcam, USA.
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PMC11024707
Accordingly, the Kruskal-Wallis analyses revealed that there were statistically significant differences between the groups in terms of mi-RNA-106a-5p 2 (p = 0.030), mi-RNA-151a 2 (p = 0.036) and miRNA-28 2 (p = 0.005) values (Table 3).
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PMC9429973
The great majority of pts destined to relapse post-allogeneic stem cell transplant (SCT) will do so within the first 12 months and survival after early relapse is dismal.
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PMC11352877
Under hypoxic conditions, HIF-1α stabilizes (i.e., it is not proteasomally degraded) and translocates to the nucleus, forming a complex with DNA and activating the gene expression necessary for adaptation to low oxygen levels .
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PMC11767793
We measured the UV–Vis absorbance of the supernatants produced during the CF process.
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PMC4112127
The viability of vector-transfected control cells was set to 100%, and the viability of BMP3-transfected cells was expressed as a percentage of formazan absorbance compared with that of control cells.
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PMC11394112
We chose the commercially available NSG-SGM3 strain, a previously reported human hematopoietic cell engraftable strain , the recently developed NSG-human IL6 strain, and the F1 strain (NSG-IL6/SGM3).
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PMC8750422
In addition, hematoxylin and eosin (H&E) staining analysis was performed in lung tissues (Figure 5d).
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PMC11237030
Optical densities (ODs) were measured at 450 nm.
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PMC11502327
Deoxyschizandrin is the main active component of Schisandra berries, which has various biological activities such as hypoglycemic, antioxidant and anti-tumor.
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PMC11750712
Three patients had no response.
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PMC11240052
Error bars reprehensive mean+ Standard deviation (SD). ***
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PMC9454852
The confocal analysis was then carried out in continuum in the same treatment conditions, and any significant oscillation in the [Ca]i was registered (Figure 5B).
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PMC11541409
Qiu, H., et al., Promising antitumor activity of olverembatinib (HQP1351) in patients (pts) with tyrosine kinase inhibitor- (TKI-) resistant succinate dehydrogenase- (SDH-) deficient gastrointestinal stromal tumor (GIST).
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PMC11185260
In contrast, serum from mice treated with radiation in combination with scL-SMARCB1 showed ALT and AST levels similar to those at baseline.
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PMC10914904
Interestingly, in cultures of peripheral blood mononuclear cells (PBMCs) from patients with multisystem inflammatory syndrome who have autosomal recessive deficiencies in the OAS/RNase L pathway and in THP-1 monocyte cells depleted of OAS/RNase L, stimulation with synthetic dsRNA exacerbates the production of the inflammatory cytokines IFN-λ1, CXCL10, and IL-6.
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PMC11470999
As shown in Figure 1A, the first‐generation diphenylbutylpiperidine antipsychotic drug PF exhibited the highest inhibitory activity.
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PMC4270159
The effect of the secondary kinase inhibitor alone will also be assessed.
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PMC9429973
65 patients (58%) had a gastrointestinal and chronic liver disorders complicated by IDA, 47 patients (42%) had a gastrointestinal and chronic liver disorders complicated by ACD.
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PMC11628309
Carvajal-Oliveros et al., 2021; De Haas et al., 2019; Hollville et al., 2020; Pinto-Costa et al., 2023; Xiong et al., 2017).
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PMC10604092
A notable factor identified by the cytokine array using the blood of the SLC-transplanted rats was NGAL.
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PMC4395774
In these gene-corrected CTL, function was equivalent to CTL generated similarly from nontransplanted, perforin sufficient, P14 transgenic mice (P14.WT).
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PMC5839394
SI was calculated as ratio between AgNPs-EPS IC50 values obtained for HB2 and SKBR3.
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PMC11502962
The risk stratification based on tumor size, tumor site, and mitotic count is often used to predict the recurrence risk.
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PMC9429973
CAPTIVATE (PCYC-1142; NCT02910583) is a multicenter phase 2 study of first-line treatment with the all-oral, once-daily, chemotherapy-free regimen of I+V in fit pts with CLL.
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PMC10366908
EGFR is a type I transmembrane glycoprotein, which is composed of an extracellular ligand-binding domain and an intracellular tyrosine kinase domain .
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PMC10158546
Cells were fixed and stained with DAPI before imaging for GFP via fluorescence microscopy.
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PMC11612508
Another crucial factor and helpful metric for determining electronic charge is the zeta potential, which may be utilized to forecast and manage the stability of colloidal suspensions or emulsions.
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PMC11539788
Additionally, the effects of RNF19A knockdown on promoting cell proliferation, migration and invasion were inhibited by the addition of LY294002, a small-molecule inhibitor of AKT/mTOR signalling.
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PMC11389534
A conditional knockout of UFBP1 in vivo resulted in significantly reduced levels of serum immunoglobulins, specific antibodies and the conversion of activated B cells into plasma cells following immunisation, despite maintaining a normal number of B cells, indicating that the expression of UFBP1 in B cells is required for the production of serum immunoglobulins and the initiation of antibody response.
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PMC11756514
No significant difference between the WT and the different mutants was observed.
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PMC10748106
The most similar to Geniposide is Catalpol, although the conformation of the sugar is very different compared to Geniposide.
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PMC11704135
For compound 1, S. epidermidis was inhibited at a concentration of 7.5 mg/mL, while K. pneumoniae, P. aeruginosa, S. haemolyticus and C. albicans were inhibited at a concentration of 15 mg/mL. In contrast, E. coli, MRSA, and P. vulgaris required a concentration of 30 mg/mL to achieve inhibition.
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PMC11345828
We hypothesized that substituting the methyl ether at this position may also improve the IV clearance; however, given the steric constraints of the nearby loop region of Mcl-1 binding site, 4-OEt was the largest ether that was well tolerated in terms of cellular potency.
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PMC10114490
FNone of the senescence-related gene sets was significantly different between PD and PD iHDF-T according to GSEA (see details in the text).
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PMC10565698
The results showed that the luminescence signal in the WT group was lower after co-transfection with miR-211-5p and circUSP10-WT than in the control group (circUSP10-WT + miR-NC).
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PMC11283030
Two small interference RNAs (siRNAs) targeting ACLY (siRNA#1 and siRNA#2), a nonsense siRNA control (si-NC) and a luciferase siRNA were constructed by Sangon (Shanghai, China).
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PMC11791478
Animals were removed from the study and euthanized if weight loss >30% (or >20% for 7 days) or study endpoint was reached [MTV of the vehicle control reached ≥1,500 mm or end of study (up to 60 days depending on the model)].
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PMC11604768
Fig. 3TRE-MPRA results using additional stimuli.a Heatmap of fold change responses in HEK293 cells across ten stimulus conditions relative to untreated cells.
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PMC11180402
Numerous studies have reported that SOX2 plays an important role in the development of various human cancers.
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PMC10891340
Premix Ex Taq (Probe qPCR) (Takara, Dalian, China) was used to determine viral genome copies.
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PMC11670954
The human epithelial Caco-2 cells utilized in this study were obtained from Kunming Cell Bank, Typical Culture Preservation Committee, Chinese Academy of Sciences.
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PMC9429973
Age of patients enrolled ranged between 19 to 81 years.
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PMC9164404
injected into the mice bearing PDX.
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PMC10840195
Fig. 5Twist overexpression in A498 cells basically eliminated the effect of sunitinib on SR-A498 cells.
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PMC11536589
Control mice (No treated) were administered vehicle at the same time points.
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PMC11686380
We found that the variant GAP43 protein is unstable in neuronal cells.
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PMC11789597
A parametric test (Student's t‐test) was used to determine the statistical significance between two groups if the data was normally distributed.
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PMC8873393
In order to reveal transcriptome landscape for lncRNAs during DNA damage repair process, we first applied cufflinks and scripture to identify novel lncRNAs (Fig. S4A).
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", ...
PMC10523483
Furthermore, knockout of H1.2 diminished the occupancy of NRF2 at ARE regions in GCLC promoter, either under basal condition or tBHP-induced oxidative stress (Fig. 5G); H1.2 overexpression increased NRF2 enrichment at these regions (SI Appendix, Fig. S12).
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PMC11397654
Finally, all the analogues of calixarenes (dihomooxacalixarenes, thiacalixarenes, calixresorcinols, azacalixarenes, calixpyrroles, pillarenes) are described in Section 4.
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PMC11754784
Flow cytometry analysis was performed by a CytoFLEX flow cytometer (Beckman Counter, Inc., USA).
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PMC10728200
J The effect of STUB1 on the ubiquitination modification of the K191 site of GPX4.
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PMC9429973
They were distributed to the following stratification groups: standard risk (56 %), intermediate risk (27 %) and high risk (17 %).
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PMC11695546
The exposing of coconut oil to ozone led to intensify the anti-biofilm role of the oil towards H. pylori.
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "The", "exposing", "of", "coconut"...
PMC9325715
The results indicate the spontaneous nature of the sorption process and the heterogeneous character of the protein surface in terms of binding energies.
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PMC11597291
The innate, hydrophobic properties of the pristine graphitic lattice of GO nanomaterials raise biosafety and poor biological stability issues, which limit their prospects for biomedical applications.
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PMC11750161
Fresh growth medium containing 20% FBS was added to each well 5 h after transfection.
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PMC9024365
M4, CT alone (n = 13), M4, CT w/o SD1 (n = 13), M4, CT w/SD1 (n = 11).
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PMC6684588
Data are means ± SEM (n = 8–9). **
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PMC11052306
In particular, both dendrimers bearing Cu(NO3)2 complexes 10-G0-[Cu(ONO2)2]4 and 10-G1-[Cu(ONO2)2]8 are highly toxic towards the healthy cell line 142BR.
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PMC11764090
Some transcription factors, such as AP2/ERF, MYB, WRKY, NAC, and bZIP, not only act as calmodulin-binding proteins during cold perception but can also play important roles in the downstream chilling-signaling pathway.
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PMC9812014
Phytochemicals such as alkaloids have a variety of biological and physiological functions and can be utilised as medications on their own .
[ { "tags": [ "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O", "O" ], "tokens": [ "Phytochemicals", "such"...
PMC8164677
In the case of combinations, cells were treated with increasing concentrations of compounds at constant ratios of their IC50 values using the sequences 0/0 h (cisplatin and wedelolactone were added immediately after seeding), 0/4 h (cisplatin immediately after seeding; wedelolactone 4 h after seeding) and 4/0 h (wedelolactone immediately after seeding; cisplatin 4 h after seeding).
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PMC9712534
Transfection of HeLa cells with the EGFP-TOP2B expression plasmid was performed using a FuGENE6 reagent (Promega, USA) according to the manufacturer’s instructions.
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PMC11012012
The Mutu III subline expressed SAP at a low level .
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PMC10748106
For this reason, we considered all 13 iridoids to perform a QSAR study using molecular descriptors related to the electronic nature of the compounds.
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PMC11792090
In summary, SAP UCAR-T cells with low immunogenicity, resistance to immune rejection, and superior survival and proliferation capabilities were successfully constructed both in vitro and in vivo.
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PMC11342785
To create the TPPP-tagBFP-FHA1 and PkAsub-DDX4-FusionRed-PixD/Citrine-PixE constructs, we first fused the PKA substrate sequence "LRRATLVD" to the N-terminus of DDX4-FusionRed-PixD and DDX4-Citrine-PixE, resulting in the PKAsub-DDX4-FusionedRed-PixD and PKAsub-DDX4-Citrine-PixE constructs.
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PMC5854676
In the case of PEG + MgO in the absence of laser irradiation, measured changes were superficial.
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PMC11279598
Antioxidant properties of Manuka honey have been attributed to its uniquely high content of polyphenols such as caffeic acid, caffeic acid phenethyl ester (CAPE), chrisin, etc.,
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PMC10728535
This study was conducted to investigate deregulated genes and signaling pathways involved in cisplatin resistance development in OC.
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PMC9351137
Two negative control samples were considered: scramble siRNAs transfected Hela cells and mocktreated HeLa cells (see “Methods”).
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PMC11583010
Ab, Antibody; LC, Light Chain; HC, Heavy Chain.
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PMC11682172
During the last wash step, cells were collected in 1.5 ml Eppendorf tubes, centrifuged for 5 min at 1000g, and the presence of residual T cells was excluded by screening for CD3, CD4, and CD8.
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PMC11047729
Their high levels can be found in some vegetable oils and certain types of seeds, nuts, and grains .
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PMC11670954
However, the inhibition of RhoA can lead to abnormal tight junction function in epithelial cells .
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PMC8557138
One such device was used to show the role of phthalimide compounds in reducing angiogenesis by observing the sprouting ability of endothelial cells after treatment (Mercurio et al. 2019).
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PMC11757131
Fatty acid oxidation is an important pathway for cellular energy production (31).
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PMC10890055
As in the previous experiment (Figure 3), the higher sensitivity of L929 compared to hFOB 1.19 cells was shown and the toxic effect at the 60 μmol∙L concentration of Zn was also confirmed for the L929 cell line (Figure 4).
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PMC8633974
Cell vitality 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay performed on the electroporated cells showed no significant change in cell viability; P=0.54 and F=0.77, and partial η of 0.22 (One-way ANOVA, n=3).
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PMC10142392
This rapid enzymatic degradation of PCL highlights its importance in terms of biodegradation .
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PMC11352746
Its impressive capabilities and wide range of components have opened new avenues of exploration and deepened our understanding of the intricate workings of cells.
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