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OTAR3088
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PMC11392912
The H&E staining shows decreased inflammation (pink color) in Clo-miR-14 treated (N=4) and in MTX (N=4) groups compared with NC (N=4) CIA (N=4), and HC (N=4) groups.
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PMC11782508
Bound azurin was eluted using a stepwise sodium chloride gradient (0, 0.2, 0.3, and 0.5 M) in the same buffer at a flow rate of 1.0 mL/min.
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PMC10969097
This limitation emphasises the need for more research and highlights the gap in the scientific evidence currently available on this specific topic.
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PMC5925824
Analysis of the PD-1/PD-L1 co-complex structure has revealed a protein-protein interface that is largely devoid of deep pockets , an interface typically amenable to targeting by antibodies.
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PMC10778532
Global gene expression profiling demonstrates an enrichment of hypoxia and EMT hallmarks in VHL-C162F cells as compared with WT VHL cells.
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PMC11599565
Cisplatin resistance appears to enhance the expression of this enzyme as noticeable by ICC staining (ICC: Phalloidin in green, TH in red and DAPI in blue).
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PMC11696576
Data are the mean ± stdev of 4 biological replicates (iROS) and 3 biological replicates (cell death).
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PMC11707577
This broadening hints at a complex interaction between the anchored molecules and the capsid surface, resembling the typical behavior of the oligothiophene molecules in the solid state.
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PMC11457557
Later investigations looked at the link between three TCF7L2 variations (rs7903146, rs12255372, and rs11196205) and coronary artery disease (CAD) in patients who had had coronary angiography .
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PMC9429973
Methods: Cardiotoxicity was defined by any combination of 1) reduction of the ejection fraction> 10% or at a value <50%) 2) electrophysiological dysfunction 3) other abnormal ultrasound, clinical or enzymatic findings.
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PMC11248911
MCF-7 (human breast adenocarcinoma), and the normal human skin fibroblast (BJ-1) cell lines were purchased from the American Type Culture Collection (Rockville, MD, USA) and maintained in RPMI-1640 medium which was supplemented with 10% heat-inactivated FBS, 100 U ml penicillin and 100 U ml streptomycin.
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PMC10891340
Viral infections can affect mitophagy, for example, the Epstein–Barr virus (EBV) inhibits the innate immunity of the host by inducing mitochondrial fission, which leads to the degradation of mitochondria wrapped in the autophagosome (Vilmen et al., 2021).
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PMC11699042
Each compound and cell line were assayed in four biological replicates.
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PMC11694346
The larger, flexible structure of GSH allows for effective intramolecular hydrogen bonding, facilitating hydrogen transfer and promoting the cleavage of the sulfonate group, which releases the fluorescent moiety more readily.
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PMC9275501
More attractively, the synergistic combination therapy with natural chemotherapy sensitizers is becoming a promising strategy for conquering multidrug resistance and reducing the side effects of chemotherapy drugs (Wang et al., 2015a).
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PMC11622247
The method is based on FIT probe methodology, which was introduced by us to increase the sequence specificity of nucleic acid detection and facilitate RNA live cell imaging.
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PMC11730320
B The CD4+ T cell CD45RA+CD28+ naive cell population is significantly low for age.
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PMC10847511
These findings indicate the association between tumor HRD status and genomic scar signatures, commonly observed in clinical samples, was replicated in the cancer cell lines.
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PMC9532503
The resulting final expression plasmids were called pMOZ-G62 H-Rit2_LC for the expression of light chain and pMOZ-G82 H-Rit2_HC for the expression of the heavy chain of anti-CD20 mAb (Fig. 1).
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PMC8708099
Cell viability was analyzed using MTT Cell Proliferation Assay Kit (Abcam, Cambridge, UK, cat.
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PMC11543853
Nevertheless, our results provide evidence that they all employ the same mechanism of endosome escape.
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PMC11599565
Before the single cell sorting, the medium was replaced with fresh medium to remove floating particles or dead cells.
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PMC11756937
Similarly, SeV, characterised by its negative single-stranded RNA, efficiently transports genes into various somatic cells, offering another non-integrative vector option.
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PMC9611901
As early as the mid-1990s, Bhat and Hadi observed that tannic acid causes DNA degradation in the presence of Cu (II) through the formation of reactive oxygen species.
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PMC11657744
Western blot analysis of Aurora A kinase level in NIH3T3 cells depleted of Cep89 or Fbf1 during ciliary disassembly.
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PMC11024707
Although several symptom clusters are found to be related to certain mi-RNA expression levels, they were not found to be significant in discriminating the patient and healthy groups.
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PMC7802142
It was also demonstrated that 74.3% HFs treated with ReN-NV were at anagen VI stage, while 40% or 48.3% HFs in PBS or 293 group, respectively (Fig. 5d).
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PMC11243198
All compounds were purchased as powders, dissolved in 100% DMSO to obtain 10 mM and 50 mM stock solutions, and stored at −20 °C.
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PMC9596868
The threshold cycle values (Ct) were determined and telomerase activity was calculated using the following formula: relative telomerase activity (RTA) of sample = 10((Ct sample-Y-intercept)/slope).
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PMC11707577
Going one step further in the complexity of the biological system, we tested the engineered bacteriophage in 3D cellular cultures, i.e spheroids from A431 cells.
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PMC11763126
C Western blot analysis of USP5 protein levels after treatment with CBDP1.
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PMC11599565
Some of them like HES1, SOX2, and PAX6 show transcriptional upregulation in qPCR analysis.
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PMC11787381
Nonetheless, bambuterol is considered to have limited capacity for crossing the BBB via the oral route.
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PMC11078693
CRITICAL: Cover the flask with aluminum foil to block light exposure.
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PMC11023271
The majority of BSA was retained in the fractions larger than 100KD.
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PMC11628904
This feature enables whole organism intravital imaging throughout the duration of wound healing allowing for investigation of the tissue response to burn injury at the cellular level.
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PMC11473843
Therefore, it would be interesting to assess whether addition of FAK inhibitors to KRAS inhibitors would be beneficial in the clinic both to potentiate their efficacy and to prevent the development of non-genetic acquired drug resistance.
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PMC10788478
Elevated LGR6 expression is associated with unfavorable prognosis in patients with lung adenocarcinoma, especially in those with wild‐type EGFR. (
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PMC11371747
After cellular uptake 5FU is metabolized via several steps to FdUMP, which is a suicide inhibitor of TS by forming a ternary complex with TS and 5,10-CH2-FH4.
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PMC11755624
Importantly, psoriasis is associated with hundreds or even thousands of differently expressed lncRNAs and the individual role of the majority of these molecules remain unknown.
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PMC8633974
Agarose gel electrophoresis demonstrated excision of a ∼2.4 kb fragment of the viral genome (Figure 5g).
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PMC11186948
Western blot of p53 protein (53 kDa) in three HD and #249 LCLs normalized on GAPDH (17 kDa), as represented in the quantification on the right (HD in grey, #249 in red).
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PMC11521786
Several studies have consistently indicated that a combination of antibodies targeting nonoverlapping epitopes elicits superior antitumor responses (such as receptor degradation, cytotoxic effects, and tumor growth reduction) compared to individual antibodies used alone, suggesting the advantages of the polyclonal antibody response (25–27).
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PMC11705862
Cyclin-dependent kinase 5 (Cdk5) is a main kinase involved in abnormal tau phosphorylation in AD brains .
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PMC11615101
The bar graph represents the infiltration of cells in the synovium of different rat groups after induction of collagen, based on calculated H&E score. (
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PMC11138140
T-test comparisons applied at each time point revealed statistically significant norepinephrine-dependent increases in optical volume as early as 2 h after the start of our imaging experiments (Figure 2F).
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PMC11787355
c Assessment of specific lysis of SCCs and FCCs upon short-term, 5 h, or long-term, 24 h, coculture with TCR-T cells, measured with cell counting by flow cytometry.
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PMC8592717
PAL was also shown to provide relief from atherosclerosis by inhibiting lipopolysaccharide (LPS)-induced endothelial apoptosis.
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PMC10956161
The TCGA database was used to analyse the survival duration of patients based on the levels PRDX6 gene expression in lung adenocarcinoma.
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PMC9429973
Indeed, we found Ku70/Ku86 proteins to interact with BCAT1.
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PMC7171147
In contrast, COL11A1 does not change glycolysis, yet preferentially upregulates fatty acid metabolism to promote cisplatin resistance in ovarian cancer cells.
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PMC9503541
Pheophorbide A is a chlorophyll catabolite that belongs to the porphyrin class .
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PMC9587298
The nomenclature adopted for the retro Diels-Alder (RDA) cleavages was adapted from the one proposed by Brüll et al., 1997, Domon and Costello, 1988.
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PMC9429973
Complications during hospitalization included respiratory failure in 84.5%, of which only 48% received VMI; reasons for not receiving VMI included a non-intubation order issued by the patient or family members and lack of infrastructure.
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PMC10470467
In the present study, to lower the dimension of 126 NRGs in the current study, we used the univariate Cox analysis, random survival forest method, and multivariate Cox analysis.
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PMC11740399
The mice in the control group received the same dose of DMSO solution.
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PMC9516447
H460 cells were transduced by lentivirus carrying shGRP78 and selected with 2μg/mL puromycin for 1 week.
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PMC9429973
K. Parmar, K. Thein, L. Tijani, S. Ball Texas Tech University Health Sciences Center, lubbock; OSHU, Oregon; Moffitt Cancer Center, Tampa, United States of America Background: Acalabrutinib is a new, selective, irreversible Bruton’s tyrosine kinase inhibitor (BTKi) approved for the treatment of adults with chronic lymphocytic leukemia (CLL).
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PMC11055323
Data are mean ± s.d.,
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PMC10582049
Survival analysis of the downloaded TCGA dataset has been performed using “survival” package.
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PMC5874706
4-Methoxy-5-isopropyl-5,6,7,8-tetrahydroindeno[1,2-b]indole-9,10-dione (THN7), identified as a potent inhibitor of CK2 (IC50 = 71 nM), was selected for an encapsulation study in order to evaluate its antiproliferative activity as THN7-loaded cyclodextrin nanoparticles.
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PMC11723210
The phase component should be changed proportionally while changing the wavelength.
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PMC11612794
Any additional analysis information for this work is available by request to the lead contact.
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PMC9712534
TOP2A is expressed in proliferating cells with peak expression at G2/M of the cell cycle.
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PMC11805741
Similarly, there were marked differences between the Control and 1200 µg groups (p = 0.00005), and between the DMSO and 1200 µg groups (p = 0.00008), both indicating lower expression levels in the 1200 µg group.
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PMC11676169
Stable BCAT1 knockdown or shRNA-expressing control cells co-expressing a plasmid-based GFP reporter specific for assessing c-NHEJ-mediated DSB repair (pimEJ5-GFP, which contains two recognition sites for I-SceI endonuclease) were generated.
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PMC11098378
Following 48 h of expression, 25 μL of supernatant media was transferred to a fresh 96-well plate and 25 μL of reaction mixtures was added to each well.
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PMC9545474
A2780 cells were either exposed to complex 6 or 8 (IC50 concentrations), or to H2O2 (200 μM) as a positive control.
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PMC11607638
Adults were maintained on an 8% (8 g /100 mL) sucrose source under a 16 h (light): 8 h (dark) light cycle as reported before.
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PMC9429973
Statistical data were obtained using R 3.5.1 software.
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PMC9429973
The mean age of patients was 44.8-year-old.
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PMC11666785
Figure 5 shows the super-resolved images reconstructed using SMLM and corrSMLM (for χ = 0.7, 0.8, 0.9).
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PMC11656657
For each sample, a minimum of 20,000 events have been analyzed.
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PMC9545714
These include IL‐6, CCL2, and CXCL1.
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PMC11788920
The cells treated with RNP1, which elevates c-Myc expression, perform as well as the control, suggesting introduction of the CRISPR complex does not reduce cell viability.
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PMC11617590
To measure the effect of SFN on the cell viability of Mia PaCa‐2 cells, the cells were seeded in a 96‐well plate at a cell count of 1 × 10 cells/200 μL/well.
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PMC11587606
Additionally, enhanced nuclear translocation of PKCζ is accompanied by an inhibition of the expression of cell tight junction and adherens junction proteins and an increase of cell mobility.
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PMC9243326
PCR cycle optimization was used to determine the optimal amplification cycle number for the downstream large-scale PCR reactions.
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PMC9429973
Homing of transduced cells from BM to the tumor site was demonstrated by the significant content of gene-marked cells in the specimens collected from 3 of the 4 analyzed pts.
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PMC10452486
Under pathological conditions (inflammation, injury, infection, and malignancy), the dimerization may involve covalent crosslinking because of exposure to oxidants (radicals, excited states, or two-electron species) that are induced by endo- or exogenous stimuli and as a result of the actions of a number of enzymes (oxidases and peroxidases).
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PMC11291697
The link between GTDC1 and altered neurodevelopment is intriguing and supported by several studies showing that glycosylation finely tunes a myriad of neural functions, such as neurite outgrowth, axon guidance, synaptogenesis, membrane excitability, neurotransmission, and neuroinflammation [30–34].
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PMC9429973
For 32 allo-HSCT recipients, aGVHD grade ≥2 incidence at day +100 was 5% vs 1.2% in arm A and arm B, respectively (RR 1.65, CI95%=0.77-2.19).
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PMC11201245
There are three possible tissue origins of the OC: surface epithelium of the ovary, epithelium of the tube, and mesothelium covering the ovary and forming abdominal cavities .
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PMC11092418
Antibodies used in Western blot included the mouse anti-EGFR antibody (clone F4) (E3138-2ml), which was obtained from Sigma-Aldrich, Merck KGaA (Dorset, UK).
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PMC11585254
Polyethylenimine for transfections was from Polysciences (23966-1).
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PMC11354225
Then, cells were washed and the quantification was performed using Iron Assay Kit (#MAK025, Sigma-Aldrich) according to the instructions of the manufacturer.
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PMC11678841
Vulvovaginal candidiasis (VVC) is a prevalent condition, affecting about 75% of women at least once in their lifetime , with recurrent VVC (RVVC) being diagnosed when more than three episodes occur per year, impacting nearly 8% of women globally .
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PMC10538569
Further, it is possible that GPI-GFP may be taken up by iDCs internally and sequestered in vesicles but not displayed on the surface of the cell membrane.
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PMC11240571
The standard curve (Supplemental Figure S5A) and data for each n = 5 (Supplemental Figure S5B,C) of ELISA results were included as Supplementary Materials.
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PMC11286266
WT full-length rCRT (UK3142) was expressed in E. coli cells (BL21 C3013).
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PMC7433824
It was previously shown that high‐dose MPA treatment caused a prominent lymphomonocytic infiltration around the C6 GBM tumor growing intracranially ; and high‐dose P4 increased the antigenicity of GBM, which may be harnessed in future endocrine‐immune protocols for GBM treatment.
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PMC9429973
While some barriers are shared across ethnic groups, including lack of information, notable differences exist between Caucasian and African respondents.
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PMC11725127
CETSA was performed as previously described.
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PMC11590870
For this assay, 1 × 10 CCD-18Co cells were seeded in each well in a sterile 96-well microplate.
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PMC9681981
To demonstrate bacteria and Caco-2 cell contact, some wells were stained with Giemsa.
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PMC11240571
In recent years, sPD-L1 has been identified in the blood of cancer patients , and many studies have been conducted to investigate its significance.
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PMC9164404
The IC50 of Abplaitn in A549 was smaller than that of the published Pt(IV) drugs (Additional file 1: Table S1) .
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PMC11767725
Dysbiosis of the skin microbiome significantly amplifies these responses, with lesional areas dominated by pathogenic bacteria such as S. aureus, S. pyogenes, and C. acnes.
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PMC11705862
All stained sections were incubated in Sudan Black (1% in 70% ethanol) (Sigma-Aldrich) for 5 min before they were rinsed and mounted with a Vectashield Set mounting medium containing DAPI (Vector Laboratories).
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PMC9429973
Neoantigens are highly selective and immunogenic tumour antigens which result from somatic hypermutations in malignant cells.
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