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OTAR3088
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PMC11525028
Total RNA was extracted from cells, and then mRNA was enriched with Poly(A)Purist MAG Kit (Invitrogen, AM1922) according to the manufacturer’s instructions.
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PMC11741577
Because various factors may affect wound healing, we investigated various mast cell-secreted factors, including tryptase, via ELISA.
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PMC10898159
Upon the IM treatment, c-KIT oncoprotein was substantially up-regulated, hinting as a protective mechanism for GIST cells to escape from TKIs, which was widely accepted as one of the major resistance mechanisms (Fig. 2).Fig.
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PMC11791478
Statistical significance for the Kruskal–Wallis Dunn or Mann–Whitney U test: *, P < 0.05; **, P < 0.01, when compared with vehicle on day 15.
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PMC9429973
Eighteen (78.3%) patients had hemorrhage requiring transfusion, with the gastrointestinal tract being the most common site.
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PMC11615743
The sensitivities of the L‐DNA device in PBS and FBS were 82.3% and 70.5%, respectively, which are greater than those of the D‐DNA devices despite the similar specificities of both devices (Figure 4G–J).
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PMC10154097
Biotinylated miR-218-5p probe and NC probe were from Sangon Biotech.
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PMC6190245
The flow cytometry histogram confirmed the induction of apoptosis in the induced toxicity.
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PMC9429973
The MYD88 and CXCR4 mutation status were captured, if available.
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PMC11727907
To investigate IGF-I signaling, ARPE-19 cells were serum-starved overnight, then exposed for 10 min to 100 nmol/L recombinant human IGF-1.
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PMC9895440
Blots were scanned by with a LI-COR Odyssey CLx imager.
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PMC6442998
YAP expression is regulated by the transcription Limb Expression 1 (LIX1) which also controls the differentiation of stomach mesenchymal precursors into SMC .
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PMC9250505
The best average response to the Olaparib, NPB, and combined NPB-Olaparib was examined utilizing Waterfall plots of response as represented in Fig. 5F.
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PMC11409031
Calculate the C-index for each prognostic model created using 10 machine learning algorithms and 113 combinations in both the training and validation datasets. (
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PMC9351137
Fixed preparations of isolated midbodies were stained with DAPI (blue), antibodies against the protein of interest (red), and anti-α-tubulin (green).
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PMC10823017
PKC-ι and PKC-ζ were immunoprecipitated (IP) from 1000μg of proteins collected from cell lysate suspensions.
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PMC11391695
The correlation between ACYP2 and TERT expression levels were analyzed by Spearman correlation coefficient which correlation coefficient is − 0.15 (p < 0.01) (Fig. S3C).
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PMC11609529
Identification of YM fusion breakpoint and detection of endogenous YM fusion transcript and protein in ES-2 ovarian cancer cell line (A) Amplification of YAP1-MAML2 (YM) fragments using primer sets spanning YAP1 exons 3–5 and MAML2 exon 2.
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PMC9429973
Pts at 193 sites in 26 countries who were FLT3-ITD+ provided informed consent and were randomized to Quiz (40 mg/day days 8-21) or PBO and were stratified by region (North America, Europe, and Asia/Other regions), age (<60 y, ≥60 y), and white blood cell count (<40×10/L, ≥40×10/L) at diagnosis.
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PMC10926885
These processes include cell proliferation, cell cycle regulation, signal transduction, inflammatory response and immunological response. ,
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PMC11476222
The optimized ganglioside profiling method provided valuable insights into the distribution of gangliosides in neuroblastoma, breast cancer, and pancreatic cancer cell lines, while maintaining high levels of accuracy and sensitivity.
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PMC11266100
This can be attributed to the presence of divalent cations (Ca and Mg), which are known to have a significant impact on GET efficiency (Neumann et al., 1996; Hristova et al., 1997; Haberl et al., 2013; Chopra et al., 2020).
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PMC9024365
We conclude that not only does the co-localization of SD4 with AMPARs increase the mean cluster size of the receptor, but colocalization with AMPARs also significantly increase the cluster size of SD4.
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PMC11344246
Following solidification of 10 μl of 80% Matrigel base layer (Corning Phenol Red Free Lot#923-100, diluted with DMEM/F12), 10 μl of cells in a 50% Matrigel/media mixture were seeded to give a final density of 1500 cells/well.
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PMC10604092
Thus, our data clearly demonstrated that SLC transplantation reduced both muscle weight and function in the rats by increasing the mRNA expression levels of the muscle-specific degradation factors (E3 ubiquitin ligases).
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PMC10938336
The DAVID database generated several GO terms and KEGG pathways, with a stringent threshold of p-values less than 0.05 applied to screen for statistically significant KEGG pathways and GO terms.
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PMC11349530
Here we will discuss another approach to cancer therapy - targeting the production of factors released by tumor cells.
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PMC11637276
All the raw data associated with this figure are available in S2 Data.
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PMC9621239
A higher number of Huh 7-TM cells were found to be labelled with Edu than Huh 7-WT cells (Figure 4H), revealing that there were more replicating cells in the Huh 7-TM cells than in the Huh 7-WT cells.
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PMC7709199
Riding H atoms were utilized during refinement for stricter geometry restraints and individual isotropic atomic displacement parameters were refined per atom.
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PMC9429973
However, previously obtained data on pharmacokinetics, as well as financial toxicity of therapy, raised the issue of studying necessity of efficacy of reduced doses of PD-1 inhibitors.
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PMC10154881
Most studies of the antitumor effects of oncolytic virotherapy have been undertaken using immunodeficient mouse models xenografted with human cancer cells, but the analysis of the adoptive immunity of oncolytic virotherapy has been limited because of a lack of suitable immunocompetent models.
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PMC10506676
The product of this gene inhibits cell apoptosis by interfering with the function of caspases.
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PMC11764090
Bermuda grass (Cynodon dactylon (L.) Pers.)
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PMC11773391
Levels of the p‐ATR, γ‐H2AX, p‐ATM, p‐Chk1 and p‐Chk2 proteins were detected in A375 cells treated with TMZ (1 mM) and vMF (1 and 2 μM) for 24 h using Western blot analysis (*p < 0.05 and **p < 0.01 compared with the control group). (
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PMC11726183
The synthesized compounds 5–25 were used against triple-negative MDA-MB-231 breast cancer cell line and human normal breast epithelial cell lines MCF-10A to check out the prohibition of cancer cell growth.
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PMC10858941
The outermost region of the tumour is supplied with nutrients and oxygen through the two side channels of the device, creating diffusion gradients similar to those occurring in the tumour in situ.
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PMC11283030
First, we compared ACLY expression profile in ccRCC and adjacent normal kidney tissues.
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PMC11342785
To facilitate direct comparison and quantification, we used cell-sorting to enrich PixD/E-positive cells with similar expression levels.
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PMC11487720
Reduced cell viability occurs during BCD.
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PMC11026382
Experimental replicates (3 total) are plotted individually.
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PMC10507284
Meanwhile, the six paired tissue data in our sequencing expressions also indicate the same trend (Figure 6B).
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PMC7812570
CEMss-CCR5 cells were transduced with GPI-m36.4/GFP and mixed with untransduced cells at a proportion of approximately 17% GFP-positive cells.
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PMC11742670
Drug_NameIC50Sulfur, precipitated 0.357777 Magnesium oxide 0.972352 Aluminum hydroxide1.475211Magnesium carbonate2.146655Cisplatin2.749105Cisplatin2.749105Acetic acid2.834914Lithium carbonate3.341439Calcium carbonate3.834431Glycine3.935773Sodium iodide3.956215Silver nitrate4.27554Magaldrate4.314857Sodium lactate6.345456Phenol6.406353Halothane7.084631Carboplatin7.517887Niacinamide8.045052Isoflurane9.004047 The predicted IC50 of the most potent drugs for the A-375 cell line.
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PMC11562028
Re-CDs can be used as a new low-toxicity and high-efficiency potential nanomedicine for the treatment of kidney cancer.
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PMC9429973
Adverse events were consistent with those reported in other crizanlizumab studies.
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PMC9429973
Finally, high cfDNA TF at diagnosis predicted for poorer prognosis as compared with low cfDNA TF (PFS at 20 months: 67% vs. 86%, p=0.05; OS at 20 months: 90% vs. 100%, p=0.04).
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PMC11569208
Viral titer and relative mRNA expression data values were log-transformed prior to statistical analysis.
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PMC10530622
Oxalobacter spp.,
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PMC9341513
For example, we found little change in CCND1 interactions if we take chromatin states from GM12878 cells for Chromosome 11 and add an insert from Chromosome 14 with states from the U266 cells (Supplemental Fig. S11A,B).
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PMC11335267
A PET-CT Albira ARS hybrid tomograph (Oncovision, Valencia, Spain) with a PET resolution of 1.5 mm (axial and transaxial fields of view, 4 and 8 cm, respectively) was used.
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PMC6934009
The data comparison was performed using ANOVA, followed by Tukey's or Dunnett's test.
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PMC11770199
It is also involved in various pathological conditions, including infections by bacteria , parasites , and viruses .
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PMC10571053
The ability of FINs to inhibit P-gp or ABCG2 was determined using the fluorescent substrates rhodamine 123 or purpuin-18, respectively.
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PMC11779605
p < 0.0001.
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PMC9031474
TRPA1 is activated by electrophilic pungent electrophiles, such as isothiocyanates, forming reversible covalent bonds to cysteine residues of the ion channel protein .
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PMC10092581
Spheroids were treated for 72 h with increasing concentrations of tested compounds.
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PMC9583612
A decomposition study of this discrepancy between measurements and inventory pointed to an underestimation of emission factors associated with tanks and equipment leaks.
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PMC9429973
Most frequent co-mut were found in ASXL1 (31%), DNMT3A (29%), and NRAS (26%).
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PMC10748106
A conformational analysis of all the simple and glycosides iridoids in their neutral and anionic form using the MMFF94 molecular mechanism model was performed .
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PMC11345828
R-X, Cs2CO3, DMF. (
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PMC8382973
Cytokine concentration was calculated by quadratic regression analysis based on logarithmic transformation optical density.
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PMC10988555
Caspase 8 activity was quantified using the caspase 8 assay kit (Abcam).
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PMC9429973
No grade 3 or higher CRS occurred in XYF19 treated patients, while Grade 3 or higher CRS was observed in 26% of patients with Tecartus , and 72% of patients with Kymriah.
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PMC10203589
RPS15A can increase the expression of FGF18 in HCC cells through Wnt/β-catenin signaling pathway.
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PMC10870498
The HEK-293T, 8505C, and BCPAP cells (5 × 10 cells/well) were inoculated in a 6-well plate (Corning, NY, USA).
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PMC11755467
For radiolabeling, 1 µL of a 1 µM of miRNA working solution was incubated with 1.5 µL ATP ([γ-32P]-6000 Ci/mmol, 10 mCi/mL, Perkin Elmer, Waltham, MA, USA), 1 µL RNase inhibitor (Promega) and 10 units T4 polynucleotide kinase (NEB) in 1× PNK buffer in a total of 10 µL reaction volume at 37 °C for 60 min.
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PMC2777936
FZD5 was down-regulated in response to differentiation of NT2/D1 cells and in human melanoma cells FZD5 activation by WNT5A had been associated with elevated metastatic potential .
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PMC11438317
When these plasmids were transfected in LYP-deficient Jurkat cells (494), SLP76 induced a higher expression of luciferase in comparison with wild type cells.
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PMC7553912
The band intensities of OSM and OSMRβ were normalized to β-actin and represented as the fold induction relative to the intensities on day 0.
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PMC9100622
The Ewing sarcoma A673 and SKNMC cell lines were obtained from the American Type Culture Collection (ATCC), the Ewing sarcoma TC71 and MHHES1 cell lines from the German Collection of Microorganisms and Cell Cultures (DSMZ), and the EW1 cell line from the International Agency for Research on Cancer (IARC).
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PMC11711663
Furthermore, the current analysis focused on extracting radiomics features from a single imaging modality, namely CT.
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PMC11739484
In the present study, the miRNet database was used to construct the miRNA-mRNA network of the MMPs.
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PMC11361748
Pretreatment of parental A431 cells with antagonists for CXCR2 (AZD5069) or CXCR3 (AMG487) , or both, prior to TPA exposure significantly reduces CM-induced neutrophil migration (Fig 3E; antagonist specificity was confirmed using fMLF; S3H Fig).
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PMC7582629
The white-light optical image and Raman imaging of MN60 cell line are additionally reported in Figure 2c.
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PMC7192625
see Supplementary Text).
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PMC9516401
The 2 method was adopted to analyze the relative expression of target genes.
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PMC10440586
The first track shows the normalized CD99 expression.
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PMC6642070
The question of the observed increased lactate production is whether it is the direct effect of statins or the metabolic compensatory effect of impaired mitochondria.
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PMC11680049
To do this, the diameter of the fungus was measured in two planes during the 21-day cultivation period .
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PMC11321678
Large‐scale gene expression profiling efforts based on microarrays and scRNA‐seq have characterized genes for molecular subgroups.
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PMC9429973
The safety and efficacy of FEDR in pts with MF previously treated with RUX are being further evaluated in the ongoing, single-arm, phase 3b FREEDOM trial (NCT03755518), which includes prospective strategies for preventing or mitigating GI AEs, thiamine decreases, and potential WE.
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PMC11583690
The percentage of dead cells was determined using formula (4).(4)I(%)=100−100×A/Cwhere I (%) is the percentage of dead cells, A is the optical density of the test sample, and C is the optical value of the control sample.
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PMC8481254
CA possesses a variety of biological properties, including antidiabetic, antiobesity, antihyperlipidemia, anti-inflammatory, and anticancer effects, and can modulate a variety of cancer-related signaling pathways and processes .
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PMC11011837
Finally, a cluster analysis was performed based on all polar metabolites detected with the Dendrogram function (see Figure 3).
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PMC11785489
We collected all contigs annotated as high confidence, full length, and nonproductive by cellranger vdj (v5.0.1).
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PMC11519583
control: n = 5, Ror2 cKO: n = 7). (*
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PMC4443654
For each of the three Hi-C eigenvector datasets corresponding to the Tier 1 ENCODE cell lines (GM12878, H1 hESC and K562) we assembled datasets of 35 matched locus-level chromatin features, including sites bound by 21 DNA binding proteins, and 11 histone modifications/variants and DNase hypersensitive sites (see Materials and methods).
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PMC11591038
Oxidative stress is involved in numerous aging-related pathological conditions, including cancer, diabetes, chronic neurodegenerative disorders, and acute brain injuries like cerebral ischemia .
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PMC3019555
In this study, we show that one important component of this cascade is Ago2, as its depletion prevented TX’s effect on HIF translation (Fig. 9), although microtubules were efficiently stabilized (not depicted).
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PMC9429973
Patients who relapsed after atleast one prior line of therapy were included.
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PMC11297139
Single-cell colonies were genotyped several times until the ARID1A levels of rescue cells were comparable to those of their WT counterparts.
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PMC11248911
Further evaluation of derivative 8 against VEGFR2 and MMP9 as biomarkers for angiogenesis and invasion showed that the selected compound had an inhibitory activity on both proteins with pIC50 values of 6.7 and 6.4, respectively.
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PMC11342785
The mean was determined from data pooled from five different images.
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PMC11770130
It is known that impaired lipolysis might be an important biological process contributing to the development of DIO.
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PMC3734024
It has been suggested that, rather than existing as a homogenous population, B cells with immunosuppressive functions can arise from both B-1 and B-2 cells .
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PMC10440586
Due to the tumor heterogeneity, we tested the hypoxia response at the single-cell level.
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PMC8073654
The rhizomes are the primary part of the plant that has been used for medicinal purposes due to their constituents with therapeutic properties, including phenylbutenoids, curcuminoids, and essential oils.
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PMC10376064
In our previous work, we have shown that DMF had no effect on cognitive impairment, β-amyloidosis, and neuroinflammation in younger APPtg female animals (endpoints at 80 and 100 days, respectively) .
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PMC9105697
Previously, we presented a methodological and clinically feasible approach for ctDNA monitoring in translocation-associated myxoid liposarcoma (MLS) patients in a routine diagnostic setting using a disease-specific hybrid capture NGS technique.
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