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PMC11499955
More recently, Carlson, Weissleder et al. nicely exploited the powerful inverse electron demand Diels–Alder reaction to quench fluorescent antibodies derivatized by trans-cyclooctenes with tetrazine quenchers.
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PMC10978090
Negative anti-PF4/H IgG levels were achieved after 5 and 3 TPE sessions, respectively and patients could beneficiate from surgery with brief heparin re-exposure without any thrombotic complication.
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PMC11754432
Moreover, intralesional injection of 15-HETE in patients with psoriasis was shown to aid resolution .
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PMC11670407
These results suggested that our OS chemotherapy regimen induced an increase in OTULIN expression.
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PMC7081114
TRIzol reagent (Shanghai Pufei Biotech Co., Ltd, Shanghai, China) was used to extract total RNA from BEL-7404 cells, which was used as a template for synthesis of cDNA using M-MLV Reverse Transcriptase (Promega, Beijing, China).
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PMC9429973
A total of 7 cycles of treatment was planned for TN patients.
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PMC11675244
However, the extensive crosstalk present between Wnt and other signaling networks (Hedgehog and Notch) complicates the development of efficient long-term therapies with minimal side-effects on normal tissues.
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PMC2118063
Affymetrix arrays were used to profile gene expression in human tonsillar B cell subsets.
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PMC11538390
P values of the meta-analysis are two-sided P values derived from a fixed-effect meta-analysis and were not adjusted for multiple testing. *
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PMC3931643
The following antibodies were used for immunoblotting: eIF4E, 4E-BP1, eIF4G, 4EBP2, MCL-1, phospho-S6 (S240/244) (Cell Signaling Technology, Beverly, MA), actin (Sigma-Aldrich).
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PMC10568263
Currently, HSP70 can be qualitatively and quantitatively validated by western blotting, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA); unfortunately, several drawbacks such as lower sensitivity, longer analysis time, complex operation, and challenges for point-of-care testing, limit the applications.
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PMC11637276
This is likely due to the abundance of fibronectin in the CDM, which is internalised in an α5β1-dependent manner in A2780-Rab25 cells , while fibronectin uptake in MDA-MB-231 cells is very low.
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PMC9784246
The absorption of each well at 540 nm was measured using the Molecular Devices Versamax Tunable Microplate Reader.
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PMC7762347
Etoposide (ET) (Santa Cruz Biotechnology, St. Cruz, CA, USA) and benzo(a)pyrene (B(a)P) (Sigma, St. Louis, MO, USA) were prepared in dimethylsulphoxide (DMSO) at 25 mg/mL and 9.9 mM, respectively.
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PMC11628904
Dashed box shows area of inset to the right.
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PMC11759543
The tumor volume (mm) was calculated using the following formula: [(width) x (lenght)]/2.
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PMC6034753
The ability of apoptin to induce apoptosis selectively in transformed cells, but not in normal cells, is thought to be related to its accumulation in the nucleus, since apoptin has a cytoplasmic location in untransformed cells.
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PMC11746948
Moreover, the overexpression of wild-type (WT) ZDHHC20 increased GPX4 palmitoylation and protein levels, but not the catalytically inactive C156S mutant of ZDHHC20 (Fig. 4f).
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PMC10178190
In this study, we utilized our cisplatin derivative, RJY13 , to explore the involvement of soluble factors secreted from MSCs and adipocyte cells in promoting OC chemoresistance.
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PMC9250505
The slides were evaluated by three independent examiners using immunoreactive score (IRS) assessment.
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PMC11803918
While previous studies have explored loading photosensitizers into exosomes for cancer therapy, our study marked the first time PDT was combined with exosomes derived from immune cells.
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PMC9926039
Data of molecular conformers RMSD: Root mean square deviation According to Table 1, molecular conformers were clustered into 19 categories.
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PMC9429973
Methods: Eligible participants received 375 mg/m rituximab on Day -14 and escalating doses of both lilotomab (60 and 100 mg/m) and Betalutin® (10, 15 and 20 MBq/kg) on Day 0.
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PMC11621493
H–J) Gene set enrichment plots of GO biological process gene sets “nuclear division,” “keratinocyte differentiation,” and “lipid catabolic process” comparing KO and WT cells in the presence of IL17A and TNFα. (
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PMC9429973
Aims: To estimate the per-patient lifetime direct healthcare cost of severe SCD from a US payer perspective using an economic model developed based on published model frameworks.
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PMC9429973
The median duration of hospitalization in patients with grade 4 neutropenia was 18 days.
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PMC7039683
While tissue-specific enhancers are rarely conserved between mammals and zebrafish (with some exceptions Visel et al., 2008), inter-species reporter tests have shown that they nonetheless can be composed of the same binding site code (Fisher et al., 2006a).
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PMC11506670
To compare means from three or more groups, analysis of variance (ANOVA) followed by Tukey’s HSD post hoc test was used to compare differences among multiple setups.
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PMC9429973
The average numbers of total, regular, and activated CAR-T cells, measured on day 5 post CAR-T-transfusion, were all higher in patients that obtained CR vs those who did not (Fig. 1B).
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PMC11747885
There are no conflicts to declare.
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PMC7612475
We have previously shown that the BRCA1 mRNA-splicing complex, which includes SF3B1, facilitates DNA repair, in part through its ability to promote the efficient splicing of genes involved in the DNA damage response (1).
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PMC9727330
Accumulating evidence showed the association between Raf/MEK/ERK signaling and the expression of Asp biosynthesis enzyme ASNS, demonstrating the sensitivity of melanoma and pancreatic cancer cells to Asp depletion, resulting in disturbing cancer cell proliferation (Pathria et al., 2019).
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PMC10761571
Previous studies showed that gingerol could induce autophagy and caspase 3-dependent apoptosis, which is in agreement with our results .
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PMC5535901
The NF-κB transcription factor is known to promote immunity through modulating induction of expression of genes and cytokines.
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PMC9429973
Significant predictors of survival were: achievment of CCyR (p=0.001), percentage of blasts in peripheral blood (p=0.008) and Hasford score (p=0.010).
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PMC11473750
For mapping the domains of RNF121 oncogenic function, SK-N-BE(2)-C cells were also transfected with either empty vector, RNF121, RNF121 Mutant 1, Mutant 2, Mutant 3 or Mutant 4 for 72 h. After siRNA or plasmid DNA transfection, media (10% FCS DMEM) was replaced every third day and colonies were left to grow for 10 days until visible colonies appeared.
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PMC9261184
Walking surveys were mostly conducted daily, where one of the above areas was the focus each day.
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PMC11549062
PTP1B deficiency decreases mouse atherosclerosis.
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PMC11519788
In line with this notion, macrophages within the TME created a tumor-supportive niche, promoting cancer stemness through paracrine or juxtacrine pathways, which in turn, to augment tumor chemoresistance and metastatic potential 47-49.
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PMC11252553
The decline in the percentages of apoptotic lymphocytes was also more significant for cysteine-treated samples than for Taurine -treated (6.69, 6.91, and 7.87 for 48 hours and 8.18, 9.31, and 12.24 for 72 hours post-irradiation at 4, 8, and 12 Gy, respectively).
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PMC11186948
Since our patient #249 had an initial clinical diagnosis of RSTS we deepened genotype-phenotype correlation performing whole RNA-seq on #249, CREBBP and HD LCLs.
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PMC9577200
Chitosan stock SOLN (2%) (w/v) was prepared using 1% aqueous acetic acid and then diluted to a final concentration of 0.2–1.5%.
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PMC11045125
Both Donor 1 and Donor 2 ΔEBNA2 EBV-infected cord blood lines express Kappa, but not Lambda, light chain, suggesting that they are clonal or oligoclonal.
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PMC11216402
All cross-fold validation sets produce similar sets of features and have very similar weight vectors.
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PMC11589157
DNA was extracted using the Blood & Cell Culture DNA Kit (Qiagen 13323, Qiagen, Germany), following the manufacturer’s recommended protocol.
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PMC11604830
More details are reported in the Data S1.
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PMC11747467
The entire RNA-seq dataset was subjected to GEO with the data series accession number GSE237789.
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PMC8358006
ChIP-seq raw reads were aligned to the human reference genome sequence version hg38 using Bowtie2 (version 2.3.4).
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PMC11727145
These results are consistent with the conditions described by most research groups.
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PMC11467964
Thus, early diagnosis and treatment are critical factors in promoting STS clinical survival prognosis.
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PMC10291556
Structures of compounds investigated in this work.
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PMC9587298
These results show that elderberry monoglycosylated anthocyanins (cyanidin-3-glucoside and cyanidin-3-sambubioside) were responsible for the MGO trapping activity of elderberry extracts, resulting in the formation of several new products, probably MGO adducts.
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PMC11367146
Cell-based ELISA was performed to investigate whether the effect of VTE is attributed to the inhibition of the MAPKs and STAT3 activation.
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PMC11705630
Double knock-down of STAT2 and IRF9 in GLDC-deficient cells rescued GLDC depletion-induced decrease in cell proliferation.
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PMC11803918
After 7 days, cells were fixed with 4% PFA solution and stained with 0.1% crystal violet.
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PMC3995603
Inhibitory effects of NO and histamine production by A. capillaris extract (AC).
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PMC11377827
PHD3: control vs. HIF1β sgRNA ****P < 0.0001, control vs. USP43 sgRNA ****P < 0.0001.
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PMC11775197
Findings on PPARβ/δ activation inducing endothelial cell proliferation and angiogenesis through a VEGF-dependent mechanism have been demonstrated .
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PMC10329237
It was followed by blocking with skim milk (5%) at room temperature (RT) for 2 hr, incubation of the membranes was performed with the indicated rabbit monoclonal antibodies (1:1000 dilution of primary antibodies incubated at 4 °C overnight, 1:2000 dilution of horseradish peroxidase-conjugated goat anti-rabbit IgG antibodies incubation at RT for 1 hr).
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PMC11530949
The observed increase in apoptosis and necrosis in the nanoparticle-treated group was significantly higher than in the extract-treated group. ***
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PMC9545474
Complex 8 was administered at 15 mg/kg/dose and complex 6 at 50 mg/kg/dose.
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PMC10530622
Moreover, the mechanisms underlying oxalate toxicity will be uncovered to provide a rational and identify putative mechanistic linkers between oxalate dysmetabolism and cardiovascular and metabolic diseases.
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PMC6889484
An internal promoter from cytomegalovirus (CMV), an internal ribosomal entry site (IRES), and a puromycin (puro)-selectable marker are present, followed by the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE).
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PMC9429973
All data will be analyzed using descriptive statistics.
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PMC8336407
In this case, the cell viability dropped to 43.48 ± 3.20% (p < 0.001).
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PMC10582049
Subpopulations of CAFs, pericytes, and smooth muscle cells are highlighted by color. (
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PMC9429973
At data cutoff (1st February, 2022), the median follow-up was 11 (2-19) months with 16 patients have completed 6 cycles.
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PMC10723784
A decrease in S phase was observed at 30 μM CBG, 5.6 ± 0.6% from 13.3 ± 0.6% in control cells, which was followed by a significant increase when CBG was used at 50 μM. Similar to S phase, a significant increase (p < .01) in G2/M phase was observed at 50 μM CBG (16.4 ± 1.9%).
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PMC9848491
Univariate Cox regression analysis was used to analyze the correlation between ER stress-related lncrnas and overall survival (OS).
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PMC9927933
Strikingly, the mice with Ube2c deletion [Kras(+)Ube2c] had remarkably reduced lung tumor burden (Figure 2, B and C, and Supplemental Figure 2C).
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PMC11496728
The transient expression detection results of pCMV-IL3-1 and pCMV-IL3-2 were 1.27- and 1.39-fold higher than those of pCMV-IL3, respectively, and the stable expression detection results of pCMV-IL3-1 and pCMV-IL3-2 were 1.43- and 1.62-fold higher than those of pCMV-IL3, respectively (Fig. 2D).
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PMC11711127
Other considerations arise from the various methodologies used to isolate Tregs and tolDCs which likely explain the many inconsistencies in their fields (187).
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PMC11755467
We next evaluated the effects of Enoxacin and nalidixic acid on HIV-1 replication in CEM-SS and MT-4 cell lines to confirm and correlate our qRT-PCR results of reduced pro-HIV-1 miR-132-3p.
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PMC7039683
Figure 2—figure supplement 1.Different clusters of H3K27Ac ChIP-seq at different developmental stages in zGPAEs.(A) ATAC-seq summit centered heatmap of H3K27Ac ChIP-seq at 4.5 hpf, 8hpf and 24hpf data from Bogdanovic et al. (2012), cluster performed by k-mean. (
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PMC11727145
The presence of these two proteins in DED with all tested bottom media, including the new simplified medium N°8, mGreen’s medium, and the two types of exudate, confirmed the good preservation of the basement membrane.
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PMC9429973
Primary endpoints were response (by PET-CT, per Lugano criteria) at day 28, best response, and safety.
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PMC11297139
Ewing’s sarcoma patient samples exhibit a stronger tendency of ARID1A condensation compared to normal counterpart.
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PMC11471176
The samples underwent incubation at ambient temperature for a duration of 15 min before addition to the wells.
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PMC11657744
Quantitation of protein levels from A. Relative Aurora A levels were normalized to β-actin and quantified by measuring band intensity at indicated time points.
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PMC9429973
The majority (55%) had the severe genotype (HbSS/ beta0-thalassemia), the remainder had genotypes HbSC or HbS/beta+-thalassemia.
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PMC9429973
These treatments, however, are generally inadequate to eradicate the LICs and the disease almost invariantly recurs with poor overall survival.
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PMC11803918
The results demonstrated that hybrid exosomes accumulated significantly more than free drug and liposome groups within melanoma tissues throughout the observation (Figures 3D, S2).
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PMC9105697
In order to obtain general information about the performance of the panels, the sequence reads were further processed with Illumina DRAGEN Enrichment Pipeline (BaseSpace, Illumina Inc., San Diego, CA, USA).
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PMC11543935
Compatibility with imaging techniques allows for the utilization of non-invasive monitoring methods.
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PMC11585254
Receptor internalization for each independent experiment was calculated as a decrease in cell surface receptor level in the CXCL13-treated condition relative to the vehicle-treated condition.
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PMC11549062
The slight recovery or enhancement of slowed‐down endocytosis resulting from PTP1B inhibition (Figure 2D), suggesting that PDGFR regulates the motility of endocytosis mainly through PTP1B activity.
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PMC9429973
Z. C. Chapanduka Haematological Pathology, Stellenbosch University, Cape Town, South Africa Background: During the bone marrow aspiration and biopsy (BMAB) procedure, patients report pain of varying intensity ranging from complete absence of pain to severe pain.
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PMC9429973
Altogether, this analysis lends confidence that allo-SCT is feasible for patients with lymphoid malignancies after PD-1 blockade.
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PMC11733919
In brief, 225 mg of poly(maleic anhydride-alt-1-octadecene) (PMAO) was added to a flask containing 195 mL of chloroform and placed in an ultrasonic bath for 30 min at room temperature.
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PMC11761919
Orally administered probiotics must traverse the acidic and alkaline environments of the gastrointestinal tract, which are rich in pepsin, pancreatic enzymes, bile and other substances.
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PMC11738017
The remaining authors declare no competing financial interests.
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PMC9646302
Our results demonstrate that LAPTM5 regulates ccRCC cell proliferation, migration, and invasion by interacting with RAC1 and activating downstream JNK/p38 pathways.
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PMC11705067
Results: It was found that cuproptosis inducer elesclomol significantly induced cell death in 786-O and A498 cells.
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PMC11569208
It is important to note that while our data supports a link between ACP2 and IFN-1 responses (Fig. S6), next steps may involve formally investigating their dependency through knockout models.
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PMC9817179
All the tested concentrations of PFA except for 0% PFA (PBS only) result in a significant change of the LLPS parameters.
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PMC11389534
However, the response rate to PD‐1/PD‐L1 blockade immunotherapy is less than 15% for most cancer types, suggesting that multiple factors impact the host's anti‐tumour immune responses.
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PMC9429973
Among FeO pts with known transfusion status, 6/56 (10.7%) had never been transfused, with all other pts having received ≥1 prior transfusion (ever-transfused).
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PMC10810426
Treatment of cells infected with MLV with AZT or the integrase inhibitor raltegravir decreased integration by –13 and 5-fold, respectively.
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PMC2118063
Stimuli provided by IL-4, CD40, and Ig cross-linking received by cells during selection in the GC not only activate IRF4 but also repress BCL6, providing a likely explanation for the contrasting expression patterns of these two genes (61).
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PMC8097906
Fig. 4Analysis of concentration dependent effects of VSM on the transcript level in RH-30 cells.
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