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PMC9429973
Aims: To investigate specific humoral and T-cell response patterns to SARS-CoV2 vaccination in MM patients treated in the community setting.
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PMC11088135
I qRT-PCR was conducted to analyze the mRNA expression of Bcl-2. **
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PMC9429973
J. Ma, Z. Ge Department of Hematology, Zhongda Hospital, School of Medicine, Southeast University, Institute of Hematology Southeast University, Nanjing; Department of Hematology, Xuzhou Central Hospital, Xuzhou, China Background: The 2017 European LeukemiaNet (ELN) risk stratification combines cytogenetic abnormalitie...
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PMC9429973
Group 3 (n=177) were randomised after course 2 if they had persisting MRD at the time of relapse.
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PMC11387401
Jurkat Trex cell lines expressing a single 2xStrepTagII-TEV-3xFLAG-tagged HIV-1 protein were created by Jäger et al, (2011), as previously described.
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PMC10291556
Hallmarks of ICD. (
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PMC9429973
Methods: Eighty pts were randomized 1:1 to receive mitapivat (5/20/50 mg twice daily) or PBO for 24 wks in the ACTIVATE trial.
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PMC7305184
This time no statistical difference between CTRL and EP group (Fig. 2) was detected.
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PMC9429973
Summary/Conclusion: The results of the current study show that at least 85% of HL pts develop a high titer of anti-spike antibodies after vaccination with 2 BNT162b2 doses.
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PMC11599565
In this work, we show a shift in the epithelial-mesenchymal transition (EMT) process towards an “ectodermal” conversion of ovarian carcinoma cells in response to cisplatin treatment, a progression we have termed epithelial-mesenchymal-ectodermal transition (EMET).
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PMC11791432
Note that ANXA1 expression is down-regulated in nasopharyngeal carcinoma and up-regulated in glioma.
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PMC9429973
Results: To validate our approach, we quantified protein expression of four selected CD markers (CD11b, CD31, CD38 and CD40) with well-known expression pattern on peripheral blood leukocytes that showed high reproducibility across centers.
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PMC9429973
These genes were selected for relative quantification; 25 control samples and 38 AML samples (28 responders and 10 non-responders to treatment) were evaluated.
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PMC11531735
Figure 4Comparison of tumor-free survival of ER121 treated groups ER121 high, ER121 low, Herceptin and PBS treated groups.
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PMC10158546
Neurons were stained with beta-III-tubulin (TUBB3; red), astrocytes were stained with glial fibrillary acidic protein (GFAP; green) and nuclei were counterstained using DAPI (Blue).
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PMC11545868
In addition, many flavonoids are known to exert anticancer effects by modulating cancer development processes such as cell proliferation, apoptosis, and angiogenesis, but the exact signaling mechanisms are not fully understood .
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PMC11547917
The extract was used for the evaluation of antioxidant activities with five different chemical assays.
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PMC10907726
Growing evidence indicated that autophagy has a significant role in tumor progression.
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PMC6222635
In the TOSY spectrum, we confirmed that 3.91, 3.76, and 3.53 indicated the hydrogen of C3, C4, and C5, and that 4.83 is the anomer hydrogen signal.
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PMC8193813
The use of a UAS platform for environmental sampling has significant advantages in many scenarios in which access to environmental samples is limited by location or other factors.
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PMC9532503
The operating parameters used for affinity chromatography are listed in Supplementary Table 1.
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PMC9429973
Secondary endpoints include CR + mCR rate, overall response rate (CR + mCR + PR + hematologic improvement), OS, PFS, LFS, and event-free survival.
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PMC9243326
The current study was the first to obtain full-length transcriptome data of the snail using third-generation sequencing technology.
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PMC9587298
Effect of methylglyoxal (MGO) and elderberry extracts using cell-models: Dose-dependent effect of exposure to MGO, for 24 h and 48 h, on Caco-2 cell viability (a1) and HepG2 cell viability (a3) and cell morphology recorded after 48 h of exposure to MGO (a2 and a4, Caco-2 and HepG2 cells, respectively), photos captured ...
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PMC9429973
Of note, this is the first time reported that a nucleoli/ribosome-gain-of-function induce bone marrow failure ribosomopathies phenotype.
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PMC10728200
Cultured cells were fixed using 4% paraformaldehyde (Sigma-Aldrich, #30525–89–4) in PBS for 20 min.
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PMC11342785
H, demonstration of the PKA-regulated synMAP circuit design from (G).
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PMC11486946
After treatment with the indicated compounds for 1 h (dashed bar), the cells were stimulated with EGF for 5 min (filled bar).
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PMC11787651
Don solved many early challenges in the analysis of histone modifications, one of which was to introduce chemical derivatization (propionylation) in the bottom–up histone PTM MS analysis workflow.
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PMC7192625
The underlying functionalities of GARDEN-NET for the production and investigation of chromatin networks and for the mapping of genome-wide features on them are provided by the ChAseR package, which can also be used on its own.
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PMC11703896
Rapamycin was docked with FKBP12 complex as a positive control to compare the binding affinities of rapamycin with other receptors.
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PMC11754291
In this study, mitotically inactivated fibroblasts were used as a feeder layer at a consistent density of 8,000 cells/cm (approximately 600,000 cells per T75 flask) across all conditions cultured with conditioned keratinocyte media, ensuring uniform baseline contributions.
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PMC4112127
Natural logarithm transformed copy numbers are displayed in Figure 3.
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PMC11802929
Single cells were assigned phenotypes by clusters determined through the leiden algorithm.
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PMC11701801
These results indicate that CBP and p300 are involved in transcriptional upregulation of the KREMEN2 gene in SMARCA4/SMARCA2-deficient and SS18–SSX fusion cells.
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PMC11474209
One commonly used approach to neutralize HABs is pre-oxidation, which deactivates cyanobacterial cells.
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PMC9429973
Findings were consistent with MPGN secondary type and moderate involvement with leukemic cells CD20 +, CD5 +, CD23 +.
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PMC9621239
After 48 h, the cycloheximide (CHX) (HY-12320, MedChemExpress, USA) was added at a final concentration of 25 μg/mL and the protein levels of HBx-WT, HBx-SM, HBx-DM, HBx-TM and MYC were detected at certain time points by the western blot assays using antibodies for HBx (ab2741, Abcam, UK) and MYC (#5605, CST, USA).
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PMC3730428
Quantitative PCR was carried out on LC480 using Syber green (Roche) according to the manufacturer's instructions.
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PMC8956657
MERS-CoV ORF4b binds IMPα without the use of a Lys at the P2 position, and instead has a Val at this position.
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PMC11661040
A total of 451 differentially expressed genes (DEGs) were identified, with 230 genes up-regulated and 221 genes down-regulated upon CGREF1 knockdown (Fig. 4A, C).
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PMC11593713
The opposite effects were observed for the human lung carcinoma A549 cell line.
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PMC11252033
The putative binding site found by FTMap and FTSite evaluations are highlighted in orange and blue mesh and in agreement with docking results. (
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PMC11185260
XTT cell viability assay at 48h after transfection.
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PMC4270159
Media only [additional].Vehicle control.1 µM sunitinib + no ligand.1 µM sunitinib + 50 ng/ml FGF.1 µM sunitinib + 50 ng/ml FGF + 0.5 µM PD173074.1 µM PD173074 + no ligand [additional].Cohort 2: M14 cell line.
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PMC10891340
The cell supernatant was collected and tested according to the previous TCID50 assay, and the virus titer was determined by observing the virus plaque through fluorescence microscopy.
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PMC10547921
Pepaxto® was first approved by the FDA in February 2021, in combination with dexamethasone, for the treatment of adult relapsed or refractory multiple myeloma.
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PMC11767725
In inflammatory conditions like AD, dysbiosis is mostly characterized by an overgrowth of S. aureus, which exacerbates inflammation and disrupts the epidermal barrier.
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PMC11611077
These findings indicate that prestin is the motor protein in OHCs.
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PMC10218459
Additionally, the combination treatments (combination with each cytostatic and CPP) showed a significant increase in the relative TUNEL (Figure 6C,G).
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PMC11674834
The molecular mechanisms linking ROS to DNA damage and cancer were recently reviewed .
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PMC6684588
In the first of a series of experiments in mice, we investigated whether intramuscular (i.m.)
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PMC9429973
We observed significant shifts in the ratio of Ly-6C+/Ly-6G- and Ly-6C(low/-)/Ly-6G+ myeloid cells, enrichment of CD8+ T cells, and depletion of mature B cells in the spleen.
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PMC11791264
F, HEK293T cells were transfected with the indicated combinations of HA-tagged SETD7, empty vector pCMV3-N-HA (HA), and MYC-tagged LC3B WT or K51M mutant.
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PMC9429973
In the phase 1/2 BRUIN study, pirtobrutinib achieved pharmacokinetic exposures that exceeded its BTK IC96 at trough, was well tolerated, and demonstrated promising efficacy in heavily pretreated, poor-prognosis MCL patients, most of whom had prior treatment with a covalent BTKi.
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PMC9429973
from the LMU Munich across four time points (day 0, 4, 14, 28) using a 92-protein multiplex proximity extension assay (Olink Bioscience).
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PMC3122064
The mean fluorescence intensity (MFI) was determined by comparison of the fluorescence intensity of unlabelled cells.
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PMC11721277
These phenomena are associated with a change in the electron density distribution in the aromatic system of the chlorin molecule and allowed us to evaluate the progress of the reaction spectrophotometrically.
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PMC11790599
mRNA purification and RT-PCR analysis were proceeded as previously described (Lin et al., 2017).
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PMC11088135
After 48 h of transfection, cells were collected and luciferase activity was assessed using a dual luciferase assay kit (Solarbio).
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PMC11705862
The mean differences between groups that have been split into two independent variables were compared with 2-way ANOVA.
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PMC3765139
Over 80% of D10 cells expressed CD133 (Figure 1A).
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PMC11612508
In this formula, Dp represents the crystallite size, 0.9 is the shape factor constant, λ denotes the X-ray wavelength, β is the full width at half maximum (FWHM) of the diffraction peak, and θ is the Bragg angle.
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PMC9583612
While technological advances have led to improvements in methane emissions measurements and monitoring, the overall effectiveness of mitigation strategies rests on quantifying spatially and temporally varying methane emissions more accurately than the current approaches.
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PMC11365427
Image created with BioRender.com with permission). (
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PMC10502403
The error bar shows the standard deviation.
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PMC4485786
Several cytokines that promote interferon-γ-dominated immune responses show antitumor activity, with interleukin 12 (IL-12) being of major importance.
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PMC11588085
As with the EB stemness index, the mesoderm index was higher in cells exposed to hypoxia than normoxia (Fig. 5D).
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PMC11656657
The variation of memory subpopulations in the control NT T-cells was only marginal, with a significant accumulation of mature effector memory (TEM) and terminally differentiated effector memory cells re-expressing CD45RA (TEMRA), as well as a reduction in the naïve subpopulation and TCM cells in the last time-points (F...
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PMC11066331
A classifier based on PLS-DA was optimized for 15 components when the root mean square error (RMSE) reached a constant value and accuracy reached 100 % (Fig. 7B and C).
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PMC11521786
On specified days, blood samples were drawn and (B) induced anti-mErbB3 PAbs were titrated by ELISA. (
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PMC11678368
Similar DFT calculations were reported earlier for the free ligands 4ClL and 5ClL .
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PMC11354239
In addition to NK cell degranulation, calcineurin can dephosphorylate the transcription factor NFAT, allowing it to translocate to the nucleus and induce the expression of IFN-γ .
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PMC11092382
A large number of studies have confirmed its potential pharmacological benefits.
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PMC11487720
However, UV induces functional WWOX-expressing cells (designated WWOXf) to undergo efficient Ca influx, NO production, and then BCD.
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PMC11674834
Anilines tended to induce only structural CA.
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PMC11078693
Use mounting media with a refraction index that matches the objective immersion media, such as PBS for water immersion lenses or oil refraction index matched mounting media for oil lenses, for example SlowFade Glass.
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PMC9429973
Regarding subgroup analysis by driving mutations, the 4 IDH1 pts obtained a CR and 3 of them received HSCT.
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PMC11107915
Dear Editor, ChatGPT, developed by OpenAI, is an advanced language model designed for natural language understanding and generation.
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PMC10006224
MCF10A cells were grown at high confluence in order to arrest them in G1.
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PMC11748618
Initial investigations focused on the closest gene, CHD1L, a helicase involved in DNA repair for its potential role in HIV integration.
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PMC11763126
However, Western blot and PCR analysis revealed that USP5 expression was not affected by CBDP1 (Fig. 8C, D).
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PMC9429973
In our case, the presence of t(8;16) could explain the WBC reduction before the administration of chemotherapy.
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PMC9429973
N. Martin, A. Xu, N. Stong, J. Rytlewski, O. Finney, T. Campbell, W. Pierceall, E. Flynt, E. Thompson, S. Kaiser Bristol Myers Squibb, Princeton, NJ; 2seventy bio, Boston, MA, United States of America Background: Multiple myeloma (MM) tumors exhibit increasing prevalence of high-risk/resistance (HR) features with each ...
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PMC7136814
After a 6-day TPA/GSK126 treatment, there was a statistically significant increase in the level of MYOG protein in MYOD1+ NOG+ cells, indicating induction of differentiation.
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PMC9545714
Subjects were to take one GTE capsule per day with food while maintaining their usual diet.
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PMC10674574
The aspects described in Table 7 may help develop protocols for preparing the cell line for validation purposes (regulatory studies), as well as for permeability studies across biological membranes (scientific studies).
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PMC11753435
Lysates were centrifuged at 15,000 rpm for 25 min at 4°C to separate the soluble and insoluble fractions.
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PMC5941560
Deletion170,397170,397Short Deletion841,0011,239,299471,460,321(70%)(47%)(0%)Short Insertion326,446616,298729,727(65%)(35%)Long Hom.
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PMC11422497
Activation of this pathway, often initiated by aberrant KIT signaling due to mutations, leads to the phosphorylation and activation of Janus kinases (JAK) and subsequent phosphorylation of Signal Transducer and Activator of Transcription (STAT) proteins.
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PMC11525028
GSEA demonstrated that the target gene of ATF4 was significantly downregulated in NAT10-KO cells (Figure 3H).
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PMC11474209
Numerous ailments, including cancer, cardiovascular disease, neurological, age-related, and metabolism disorders, can be brought on by epigenetic alterations that result in mitochondrial malfunction .
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PMC9429973
One out of 11 (9.0%, mitapivat) and 6 out of 10 (60%, PBO) pts who received a transfusion in the 52 wks prior to study treatment required an on-study transfusion (Table).
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PMC11402217
By contrast, the XD23-treated mice demonstrated improved survival, with no deaths until days 23 and 27 for low and high doses, respectively, culminating in a 75% survival rate, a marked enhancement over MTX (Figure 5B).
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PMC11772585
A CCK8 assay revealed reduced HSC-1 and A431 cell viability after METTL1 knockdown (Fig. 2D).
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PMC9105697
The translocation is unique and is considered the main oncogenic driver in SS .
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PMC5963610
Control samples were prepared with the second antibody alone.
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PMC5173112
In current study, we found that depletion of USP21 significantly repress the mRNA expression of IL-8 in RCC cell lines, confirming an essential role of USP21 in regulating inflammation pathway.
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PMC9960565
The results are presented in Table 3 as the half-maximal inhibitory concentration (IC50) values and can be analyzed regarding the cell line, the dendrimer generation, and the type of coordination to cisplatin.
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PMC11047729
Moreover, this substance impairs metastasis and angiogenesis.
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