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11160922 | Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control protein. | There is still a lack of information on the specific characteristics of DNA-binding proteins from hyperthermophiles. Here we report on the product of the gene orf56 from plasmid pRN1 of the acidophilic and thermophilic archaeon Sulfolobus islandicus. orf56 has not been characterised yet but low sequence similarily to s... |
11160909 | Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes. | The uptake and expression of extracellular DNA has been established as a mechanism for horizontal transfer of genes between bacterial species. Such transfer can support acquisition of advantageous elements, including determinants that affect the interactions between infectious organisms and their hosts. Here we show th... |
11165095 | Solubilization of tropicamide by hydroxypropyl-beta-cyclodextrin and water-soluble polymers: in vitro/in vivo studies. | 1% (w/v) aqueous solutions of tropicamide (TR), a poorly water-soluble mydriatic-cycloplegic drug, are usually obtained by adjusting the pH to approximately 5.0, at the expense, however, of ocular tolerance and bioavailability. The capacity of hydroxypropyl-beta-cyclodextrin (HP-beta-CD) to solubilize TR in pH 7.4 0.02... |
11166216 | Coiled coils: a highly versatile protein folding motif. | The alpha-helical coiled coil is one of the principal subunit oligomerization motifs in proteins. Its most characteristic feature is a heptad repeat pattern of primarily apolar residues that constitute the oligomer interface. Despite its simplicity, it is a highly versatile folding motif: coiled-coil-containing protein... |
11181031 | Peptide nucleic acid-assisted topological labeling of duplex dna. | Peptide nucleic acids (PNAs) are a family of synthetic polyamide mimics of nucleic acids that offer a variety of applications. Pyrimidine bis-PNAs can be used for rational design of novel interlocked DNA nanostructures, earring labels, representing locked pseudorotaxanes or locked catenanes. These structures are create... |
11161211 | Interwoven metal-organic framework on a periodic minimal surface with extra-large pores. | Interpenetration (catenation) has long been considered a major impediment in the achievement of stable and porous crystalline structures. A strategy for the design of highly porous and structurally stable networks makes use of metal-organic building blocks that can be assembled on a triply periodic P-minimal geometric ... |
11161022 | Two types of pea leghemoglobin genes showing different O2-binding affinities and distinct patterns of spatial expression in nodules. | Five distinct cDNA clones for leghemoglobin (Lb) were isolated from pea (Pisum sativum) nodules. They were classified into two groups designated PsLbA and PsLbB according to sequence homology, O(2)-binding affinities of the recombinant proteins, and in situ localization of the mRNAs. The PsLbB group was comprised of fo... |
11174081 | Ursodeoxycholic acid complexation with 2-hydroxypropyl-beta-cyclodextrin increases ursodeoxycholic acid biliary excretion after single oral administration in rats. | Complexation of ursodeoxycholic acid (UDCA) with 2-hydroxypropyl-beta-cyclodextrin (HPbetaCD) improves the water solubility and the dissolution rate of UDCA and may therefore increase its bioavailability. We compared the amount and the rate of biliary excretion of UDCA and biliary lipid secretion after a single oral ad... |
11169530 | Interaction of L-tryptophan with alpha-cyclodextrin: studies with calorimetry and proton nuclear magnetic resonance spectroscopy. | The interaction of L-tryptophan with alpha-cyclodextrin was investigated in a 0.1 M phosphate buffer at pH 7.4 with a LKB 2277 microcalorimeter, using flow mixed mode at 25 degrees C. The thermodynamic parameters for inclusion complex formation obtained are as follows; DeltaG(0) = - 7.03 kJ/mol (K = 17.0), DeltaH(0) = ... |
11162119 | Probing the kinetics of formation of the bacteriophage MS2 translational operator complex: identification of a protein conformer unable to bind RNA. | We have investigated the kinetics of complex formation between bacteriophage MS2 coat protein subunits and synthetic RNA fragments encompassing the natural translational operator site, or the consensus sequences of three distinct RNA aptamer families, which are known to bind to the same site on the protein. Reactions w... |
11181755 | Cellular cholesterol efflux is modulated by phospholipid-derived signaling molecules in familial HDL deficiency/Tangier disease fibroblasts. | Familial HDL deficiency (FHD) is the heterozygous form of Tangier disease (TD). Mutations of the ABCA1 gene cause FHD and TD. FHD/TD cells are unable to normally efflux cholesterol onto nascent HDL particles, which are rapidly catabolized. TD fibroblasts have an abnormal pattern of PLC and PLD activation following cell... |
11181650 | Role for HLA class II molecules in HIV-1 suppression and cellular immunity following antiretroviral treatment. | HIV-1-infected patients treated early with combination antiretrovirals respond favorably, but not all maintain viral suppression and improved HIV-specific Th function. To understand if genetic factors contribute to this variation, we prospectively evaluated over 18 months 21 early-treated patients stratified by alleles... |
11157955 | Influence of the nucleoid on placement of FtsZ and MinE rings in Escherichia coli. | We previously presented evidence that replicating but unsegregated nucleoids, along with the Min system, act as topological inhibitors to restrict assembly of the FtsZ ring (Z ring) to discrete sites in the cell. To test if nonreplicating nucleoids have similar exclusion effects, we examined Z rings in dnaA (temperatur... |
11157932 | Role of CspC and CspE in regulation of expression of RpoS and UspA, the stress response proteins in Escherichia coli. | Nine homologous proteins, CspA to CspI, constitute the CspA family of Escherichia coli. Recent studies are aimed at elucidating the individual cellular functions of these proteins. Two members of this family, CspC and CspE, are constitutively produced at 37 degrees C. In the present study, these two proteins were evalu... |
11157927 | Genetic analysis of assembly of the Salmonella enterica serovar Typhimurium type III secretion-associated needle complex. | Several pathogenic bacteria have evolved a specialized protein secretion system termed type III to secrete and deliver effector proteins into eukaryotic host cells. Salmonella enterica serovar Typhimurium uses one such system to mediate entry into nonphagocytic cells. This system is composed of more than 20 proteins wh... |
11160018 | Amino-terminal hydrophobic region of Helicobacter pylori vacuolating cytotoxin (VacA) mediates transmembrane protein dimerization. | Helicobacter pylori VacA is a secreted protein toxin that forms channels in lipid bilayers and induces multiple structural and functional alterations in eukaryotic cells. A unique hydrophobic segment at the amino terminus of VacA contains three tandem repeats of a GxxxG motif that is characteristic of transmembrane dim... |
11159961 | Vacuolating cytotoxin of Helicobacter pylori plays a role during colonization in a mouse model of infection. | Helicobacter pylori, the causative agent of gastritis and ulcer disease in humans, secretes a toxin called VacA (vacuolating cytotoxin) into culture supernatants. VacA was initially characterized and purified on the basis of its ability to induce the formation of intracellular vacuoles in tissue culture cells. H. pylor... |
11156990 | Complex organization of promoter and enhancer elements regulate the tissue- and developmental stage-specific expression of the Drosophila melanogaster Gld gene. | The Drosophila melanogaster Gld gene has multiple and diverse developmental and physiological functions. We report herein that interactions among proximal promoter elements and a cluster of intronically located enhancers and silencers specify the complex regulation of Gld that underlies its diverse functions. Gld expre... |
11161728 | Three-dimensional supramolecular organization of the extracellular matrix in human and rabbit corneal stroma, as revealed by ultrarapid-freezing and deep-etching methods. | The present work was carried out to clarify the three-dimensional fine structure of extracellular matrix in the cornea, using ultrarapid-freezing and deep-etching methods for electron microscopy. Fresh and glutaraldehyde-fixed samples of human and rabbit posterior corneas were ultrarapidly-frozen onto a copper block co... |
11168426 | Mechanism of porcine pancreatic alpha-amylase. Inhibition of amylose and maltopentaose hydrolysis by alpha-, beta- and gamma-cyclodextrins. | The effects of alpha-, beta- and gamma-cyclodextrins on the amylose and maltopentaose hydrolysis catalysed by porcine pancreatic alpha-amylase (PPA) were investigated. The results of the statistical analysis performed on the kinetic data using the general initial velocity equation of a one-substrate reaction in the pre... |
11179230 | The adenovirus E4-ORF4 splicing enhancer protein interacts with a subset of phosphorylated SR proteins. | SR proteins purified from uninfected HeLa cells inhibit adenovirus IIIa pre-mRNA splicing by binding to the intronic IIIa repressor element (3RE). In contrast, SR proteins purified from late adenovirus-infected cells are functionally inactivated as splicing repressor proteins by a virus-induced dephosphorylation. We ha... |
11169146 | S-100-positive nerve fibers in hepatocellular carcinoma and intrahepatic cholangiocarcinoma: an immunohistochemical study. | The aim of the present study was to determine whether or not liver carcinomas are innervated, since there have been no previous reports on the distribution of nerve fibers in human hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). We investigated nerve fibers by immunohistochemical and morphomet... |
11180297 | Separation of chiral 4-substituted imidazole derivatives by cyclodextrin-modified capillary electrophoresis. | Enantiomeric separations by cyclodextrin-modified capillary electrophoresis of chiral carbamates containing a 4-substituted imidazole heterocycle, pharmacologically acting as histamine H3-receptor antagonists, were carried out. Various cyclodextrins (alpha-, beta- and substituted beta-cyclodextrins) were investigated. ... |
11179423 | Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles. | Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA ... |
11170255 | Lipase-catalyzed irreversible transesterification of 1-(2-furyl)ethanol using isopropenyl acetate. | Asymmetric acetylation of racemic 1-(2-furyl)ethanol with the innocuous acyl donor isopropenyl acetate catalyzed by lipases in organic media afforded the chiral alcohol and acetate in high enantiomeric excess (up to 99%). The effect of molecular sieves as well as organic solvents on the kinetic resolution were studied.... |
11170254 | Circular dichroism of host-guest complexes of achiral pyridino- and phenazino-18-crown-6 ligands with the enantiomers of chiral aralkyl ammonium salts. | Circular dichroism (CD) spectroscopy was used for distinguishing different types of chiral interactions in host-guest complexes of achiral pyridino- and phenazino-18-crown-6 ligands with chiral aralkyl ammonium salts. The general feature of the CD spectra of many homochiral (e.g., (R,R)-host and (R)-guest) and heteroch... |
11170248 | Use of atmospheric pressure ionization mass spectrometry in enantioselective liquid chromatography. | Recent advances in mass spectrometry have rendered it an attractive and versatile tool in industrial and academic research laboratories. As a part of this rapid growth, a considerable body of literature has been devoted to the application of mass spectrometry in studies involving enantioselectivity, molecular recogniti... |
11173372 | Ethane-1,2-diphosphonic acid as a building block in supramolecular chemistry; a pillared-layer framework and framework-encapsulated cations. | The 1:1 adduct of piperazine and ethane-1,2-diphosphonic acid is a salt [C(4)H(12)N(2)](2+).[C(2)H(6)O(6)P(2)](2-), in which both ions lie across centres of inversion in space group P2(1)/c. The anions are linked by a single type of O-H...O hydrogen bond [O...O, 2.562 (3) A; H...O, 1.73 A, O-H...O, 169 degrees ] into (... |
11167081 | Enzyme fluorescence as a sensing tool: new perspectives in biotechnology. | The technology for fluorescence protein-sensing is advancing rapidly owing to the continued introduction of new concepts, new fluorophores, and proteins engineered for sensing-specific analytes. Concerns about the reversibility and selectivity of engineered proteins are being addressed by developing biosensors that are... |
11167074 | Applications of peptide arrays prepared by the SPOT-technology. | The growing range of applications for peptide arrays synthesized on coherent membranes by the SPOT-synthesis method proves they have emerged as a powerful proteomics technique to study molecular recognition events and identify biologically active peptides. Several developments, such as the introduction of novel polymer... |
11159466 | A simple approach to membrane protein secondary structure and topology based on NMR spectroscopy. | This paper describes a simple, qualitative approach for the determination of membrane protein secondary structure and topology in lipid bilayer membranes. The approach is based on the observation of wheel-like resonance patterns observed in the NMR 1H-15N/15N polarization inversion with spin exchange at the magic angle... |
11159459 | Efficient energy transfer from the carotenoid S(2) state in a photosynthetic light-harvesting complex. | Previously, the spatial arrangement of the carotenoid and bacteriochlorophyll molecules in the peripheral light-harvesting (LH2) complex from Rhodopseudomonas acidophila strain 10050 has been determined at high resolution. Here, we have time resolved the energy transfer steps that occur between the carotenoid's initial... |
11159453 | Monitoring DNA/poly-L-lysine polyplex formation with time-resolved multiangle laser light scattering. | Nonviral DNA complexes show promise as alternative and attractive gene delivery vectors for treating genetic diseases. Nonviral DNA complexes are typically formed by combining DNA with various condensing/complexing agents such as lipids, polyelectrolytes, polymers, polypeptides, and surfactants in solution. DNA/poly-L-... |
11170476 | Biophysical analysis of the endoplasmic reticulum-resident chaperone/heat shock protein gp96/GRP94 and its complex with peptide antigen. | Animals vaccinated with heat shock protein (HSP)--peptide complexes develop specific protective immunity against cancers from which the HSPs were originally isolated. This autologous specific immunity has been demonstrated using a number of HSP--peptide antigen complexes. A prototypical HSP-based cancer vaccine is the ... |
11171078 | Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains. | Membrane peptidases play important roles in cell activation, proliferation and communication. Human fibroblast-like synoviocytes express considerable amounts of aminopeptidase N/CD13, dipeptidyl peptidase IV/CD26, and neprilysin/CD10, transmembrane proteins previously proposed to be involved in the regulation of intra-... |
11171037 | Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. | Protein phosphatase 2A (PP2A) comprises a family of serine/threonine phosphatases, minimally containing a well conserved catalytic subunit, the activity of which is highly regulated. Regulation is accomplished mainly by members of a family of regulatory subunits, which determine the substrate specificity, (sub)cellular... |
11157277 | Molecular characterization of cycloinulooligosaccharide fructanotransferase from Bacillus macerans. | Cycloinulooligosaccharide fructanotransferase (CFTase) converts inulin into cyclooligosaccharides of beta-(2-->1)-linked D-fructofuranose by catalyzing an intramolecular transfructosylation reaction. The CFTase gene was cloned and characterized from Bacillus macerans CFC1. The CFTase gene encoded a polypeptide of 1,333... |
11157238 | Genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from Rhodococcus erythropolis DCL14. | A monoterpene epsilon-lactone hydrolase (MLH) from Rhodococcus erythropolis DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol, was purified to apparent homogeneity. It is a monomeric enzyme of 31 kDa that is active with (... |
11158738 | High-affinity binding of silybin derivatives to the nucleotide-binding domain of a Leishmania tropica P-glycoprotein-like transporter and chemosensitization of a multidrug-resistant parasite to daunomycin. | In order to overcome the multidrug resistance mediated by P-glycoprotein-like transporters in Leishmania spp., we have studied the effects produced by derivatives of the flavanolignan silybin and related compounds lacking the monolignol unit on (i) the affinity of binding to a recombinant C-terminal nucleotide-binding ... |
11272310 | Development of a capillary electrophoresis assay for the determination of carvedilol enantiomers in serum using cyclodextrins. | A capillary electrophoresis method using cyclodextrins as the chiral selectors was developed for the determination of carvedilol enantiomers in serum. Several types of cyclodextrins were evaluated. The effect of cyclodextrin concentration on enantiomer resolution was investigated. Best results were obtained using 10 mM... |
11217748 | Enhanced detection of live bacteria using a dendrimer thin film in an optical biosensor. | Here we describe the detection of live Pseudomonas aeruginosa using a sensing film containing a fourth-generation hydroxy-terminated polyamidoamine (PAMAM) dendrimer (i.e., G4-OH) and SYTOX Green fluorescent nucleic acid stain. The films are configured on simple, disposable plastic coupons or optical fibers and are int... |
11217747 | Optical sensors for detection of bacteria. 1. General concepts and initial development. | The concept of using immobilized nucleic acid stains as detection chemistry to fabricate optical bacterial sensors is first demonstrated. SYTO 13 (a green fluorescent cell stain) is used as the molecular recognition element and fluorescent reporter in the sensor. The sensor responds to aqueous and aerosolized bacterial... |
11217104 | Preparation of a novel aggregate like sugar-ball micelle composed of poly(methylglutamate) and poly(ethyleneglycol) modified by lactose and its molecular recognition by lectin. | We report the preparation and characteristics of a novel micellar aggregate of an amphiphilic diblock copolymer, poly(methylglutamate) (PMG)-poly(ethyleneglycol) (PEG), whose terminus was modified by lactose lactone (LA). Due to the terminal LA moiety, this aggregate could be specifically recognized by RCA120 lectin. P... |
11154274 | Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI. | The ATPase ISWI can be considered the catalytic core of several multiprotein nucleosome remodeling machines. Alone or in the context of nucleosome remodeling factor, the chromatin accessibility complex (CHRAC), or ACF, ISWI catalyzes a number of ATP-dependent transitions of chromatin structure that are currently best e... |
10936461 | Fibrillin-rich microfibrils of the extracellular matrix: ultrastructure and assembly. | Fibrillin-rich microfibrils are a unique class of extensible connective tissue macromolecules. Their critical contribution to the establishment and maintenance of diverse extracellular matrices was underlined by the linkage of their principal structural component fibrillin to Marfan syndrome, a heritable connective tis... |
11278383 | Determination of photosystem II subunits by matrix-assisted laser desorption/ionization mass spectrometry. | Photosystem II of higher plants and cyanobacteria is composed of more than 20 polypeptide subunits. The pronounced hydrophobicity of these proteins hinders their purification and subsequent analysis by mass spectrometry. This paper reports the results obtained by application of matrix-assisted laser desorption/ionizati... |
11818656 | [Ni(cyclam)(OCOR)2], a finite molecular complex: hydrogen-bonded supramolecular aggregation in one, two and three dimensions, and coordination polymers in one and two dimensions. | In the complexes [Ni(cyclam)(OCOR)2] (cyclam = 1,4,8,11-tetraazacyclotetradecane), where (RCOO)- is 2-naphthoate [bis-(2-naphthoato)-1,4,8,11-tetraazacyclotetradecanenickel(II), (I), monoclinic P2(1)/c, Z' = 0.5], 3,5-dinitrobenzoate [bis-(3,5-dinitrobenzoato)-1,4,8,11-tetraazacyclotetradecanenickel(II), (II), triclini... |
11278358 | Hypochlorite-modified low density lipoprotein inhibits nitric oxide synthesis in endothelial cells via an intracellular dislocalization of endothelial nitric-oxide synthase. | Hypochlorous acid/hypochlorite, generated by the myeloperoxidase/H(2)O(2)/halide system of activated phagocytes, has been shown to oxidize/modify low density lipoprotein (LDL) in vitro and may be involved in the formation of atherogenic lipoproteins in vivo. Accordingly, hypochlorite-modified (lipo)proteins have been d... |
11160995 | Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2. | Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, f... |
14727890 | Solubilization of cyclosporin A. | This study investigated the solubilization of cyclosporin A (CsA), a neutral undecapeptide, by cosolvency, micellization, and complexation. Cosolvents (ethanol, propylene glycol, polyethylene glycol, tetrahydrofurfuryl alcohol polyethyleneglycol ether, and glycerin), surfactants (polyoxyethylene sorbitan monooleate [(T... |
11158624 | Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method. | The nicotinic acetylcholine (ACh) receptors cycle among classes of nonconducting resting states, conducting open states, and nonconducting desensitized states. We previously probed the structure of the mouse-muscle ACh receptor channel in the resting state obtained in the absence of agonist and in the open states obtai... |
11278278 | Comodulation of CXCR4 and CD26 in human lymphocytes. | We provide convergent and multiple evidence for a CD26/CXCR4 interaction. Thus, CD26 codistributes with CXCR4, and both coimmunoprecipitate from membranes of T (CD4(+)) and B (CD4(-)) cell lines. Upon induction with stromal cell-derived factor 1alpha (SDF-1alpha), CD26 is cointernalized with CXCR4. CXCR4-mediated down-... |
11148208 | Binding of antigenic peptide to the endoplasmic reticulum-resident protein gp96/GRP94 heat shock chaperone occurs in higher order complexes. Essential role of some aromatic amino acid residues in the peptide-binding site. | Vaccination with heat shock protein gp96-antigenic peptide complexes produces a powerful specific immune response against cancers and infectious diseases in some experimental animal models, and gp96-peptide complexes are now being tested in human clinical trials. gp96 appears to serve as a natural adjuvant for chaperon... |
38239932 | Probing Adenosine and P2 Receptors: Design of Novel Purines and Nonpurines as Selective Ligands. | Recent approaches to the design of selective agonists and antagonists at adenosine (AR) and P2 receptors include both modifying known receptor ligands and searching for structurally diverse antagonists. The ribose-like moiety of nucleoside/nucleotide derivatives was rigidified with a methanocarba (mc) modification, to ... |
38239931 | Two Distinct P2Y Receptors Are Involved in Purine- and Pyrimidine-Evoked Ca<sup>2+</sup> Elevation in Mammalian Brain Astrocytic Cultures. | ATP and 2-methyl-thio-ATP (2-Me-SATP) increase cytosolic calcium concentrations ([Ca<sup>2+</sup>]<sub>i</sub>) in rat striatal astrocytes (Centemeri et al. [1997] Br J Pharmacol 121:1700-1706). The aim of the present study was to: (1) characterize pyrimidine-induced [Ca<sup>2+</sup>]<sub>i</sub> increases in the same ... |
29712413 | An Artificial Ion Channel Formed by a Macrocyclic Resorcin[4]arene with Amphiphilic Cholic Acid Ether Groups. | Tail-to-tail assembly of two molecules of a resorcin[4]arene resulted in a bilayer membrane with an intramembrane ion channel. The steroidal tails introduced here have the advantages of giving a long-lived open state of the pore (2.5-4.5 s, as opposed to tens of milliseconds for alkyl tails) and of allowing the hydroph... |
29712411 | β-Cyclodextrin-Assisted Intervalence Charge Transfer in Mixed-Valent [2]Rotaxane Complexes Having Metal Centers Linked by an Interrupted π-Electron System. | Fe<sup>II</sup> to Ru<sup>III</sup> electron transfer is initiated by the inclusion of saturated bridging ligands into the β-cyclodextrin (CD) cavity of [2]rotaxane complexes and is observed as an intervalence charge transfer (IVCT) band in the electronic spectra. The rotaxanes are synthesized by using Ru<sup>III</sup>... |
29712406 | A Two-Dimensional Polyrotaxane with Large Cavities and Channels: A Novel Approach to Metal-Organic Open-Frameworks by Using Supramolecular Building Blocks. | A seven-membered molecular necklace composed of six copper ions and six pseudorotaxane units behaves as a secondary building block in the formation of a two-dimensional polyrotaxane network with large voids. This novel metal-organic framework allows size-selective anion exchange as well as the exchange of coordinated l... |
29712380 | Identification and Isolation of a Receptor for N-Methyl Alkylammonium Salts: Molecular Amplification in a Pseudo-peptide Dynamic Combinatorial Library. | A cyclic pseudo-peptide receptor for acetylcholine has been amplified and isolated from a dynamic combinatorial library by virtue of templated stabilization under thermodynamic control (see scheme, TFA=trifluoroacetic acid). This is a demonstration of significant molecular amplification in dynamic systems to evolve a n... |
27500021 | Materials Research With Neutrons at NIST. | The NIST Materials Science and Engineering Laboratory works with industry, standards bodies, universities, and other government laboratories to improve the nation's measurements and standards infrastructure for materials. An increasingly important component of this effort is carried out at the NIST Center for Neutron R... |
23105285 | An overview of monoclonal gammapathies. | The neoplastic proliferation of single clones of plasma cells causes synthesis of very large amount of monoclonal immunoglobulins consisting of only one type of heavy either the gamma, alpha, mu, delta or epsilon chain or only kappa or lambda light chains. Each monoclonal immunolobulin differs idiotypically from each o... |
21337168 | Molecular recognition mechanisms of meningococci : identifying receptor-ligand pairs involved in microbe-host interactions. | Neisseria meningitidis is a human specific pathogen and resides primarily in the nasopharynx of its host. The molecular-recognition mechanisms that operate at the host-microbe interface to impart such precise host/tissue specificity are not fully defined. Given the host muco-cilliary clearance mechanisms, an obvious pr... |
21336752 | Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics. | The design of novel vaccines and strategies to combat infectious disease requires an understanding of the interactions between pathogen and host. Biological interactions in vivo often rely on specific recognition mechanisms that begin with a binding step. The development of biosensor technology has allowed the real-tim... |
21336751 | Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins. | An understanding of the molecular basis for the recognition of outer-membrane proteins (OMPs) by antibody is an important goal in the development of a more rational approach to vaccine design. X-ray crystallography has been outstandingly successful in delineating the detailed chemical interactions that are responsible ... |
21318743 | Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (L-lysine) Dendrimer with Plasmid DNA. | Research and development related to nonviral gene carriers comprising chemically synthesized molecules has increased enormously during the past decade. Polycationic polymers and cationic lipids have constituted the main themes of the studies. Various polymers from synthetic to naturally occurring ones have been introdu... |
21318742 | Cationic α-Helical Peptides for Gene Delivery into Cells. | Development of nonviral gene transfer techniques has progressed, particularly the use of several kinds of cationic lipids and cationic polymers such as polylysine derivatives, polyethyleneimines, polyamidoamine dendrimers, and so on, which electrostatically form a complex with the negatively charged DNA, which can be t... |
20428437 | Regulation of cardiac cushion development by hyaluronan. | Hyaluronan is an extracellular matrix component implicated in expansion of the extracellular space, organization of supramolecular architecture, cell motility, proliferation, tumour metastases and wound healing. Hyaluronan is highly expressed in the developing heart but it is only a minor component of the mature heart.... |
19530915 | PHARMACOKINETIC STUDIES OF IN-SITU LIPOSOMAL PREPARATION CONTAINING AMPHOTERICIN B COMPLEXED WITH DIFFERENT CHEMICALLY MODIFIED beta-CYCLODEXTRINS. | The purpose of this study was to evaluate the influence of chemically modified beta-cyclodextrin (beta-CD) which could affect the in-vivo stabilization of liposomal preparation derived from proliposome entrapping inclusion complex of amphotericin B (AmB) with beta-CD. A series of liposomal AmB formulations with varying... |
18968185 | Spectrofluorimetric study of the beta-cyclodextrin:vitamin K3 complex and determination of vitamin K3. | Vitamin K(3) (menadione) is an oil-soluble vitamin and not naturally fluorescent but yields fluorescence when it is reduced. However, it is possible to yield a fluorescent derivative in the region of 407 nm in aqueous medium when complexed to beta-cyclodextrin (CD). A 1:1 stoichiometric ratio and a formation constant o... |
18968196 | Electrocatalysis of chloroacetic acids (mono-, di- and tri-) at a C60-[dimethyl-(beta-cyclodextrin)]2 and nafion chemically modified electrode. | The C(60)-[dimethyl-(beta-cyclodextrin)](2) and nafion chemically modified electrode (CME) exhibits one electroreduction peak and two electro-oxidation peaks in a mixed solvent of water and acetonitrile (3:2, v/v) containing tetra-butylammonium perchlorate. The reduction of chloroacetic acids (mono-, di- and tri-) can ... |
18968175 | Room-temperature phosphorescence of 6-bromo-2-naphthol included in beta-cyclodextrin in the presence of cyclohexane. | The formation constant for a 1:1 binary complex between 6-bromo-2-naphthol (6B2N) and beta-cyclodextrin (CD) in aqueous solution was determined by both absorptimetric and fluorimetric methods. Room-temperature phosphorescence (RTP) was induced by adding small amounts of different apolar liquids as the third component, ... |
18924707 | Speciation of inorganic selenium and selenoamino acids by an HPLC-UV-HG-AFS system. | For the on-line speciation of selenocystine (SeCys), selenomethionine (SeMet), selenoethionine (SeEt), selenite (Se(IV)) and selenate (Se(VI)), a high-performance liquid chromatography-UV irradiation-hydride generation-atomic fluorescence spectro- metric method is described. Separation was carried out on a conventional... |
18629248 | iSPOT: a web tool for the analysis and recognition of protein domain specificity. | Methods that aim at predicting interaction partners are very likely to play an important role in the interpretation of genomic information. iSPOT (iSpecificity Prediction Of Target) is a web tool (accessible at http://cbm.bio.uniroma2.it/iSPOT) developed for the prediction of protein-protein interaction mediated by fam... |
18475989 | Antiandrogen and Antimicrobial Aspects of Coordination Compounds of Palladium(II), Platinum(II) and Lead(II). | Synthesis, characterization and antimicrobial activities of an interesting class of biologically potent macrocyclic complexes have been carried out. All the complexes have been evaluated for their antimicrobial effects on different species of pathogenic fungi and bacteria. The testicular sperm density, testicular sperm... |
18475986 | Interaction of Ruthenium(II)-dipyridophenazine Complexes with CT-DNA: Effects of the Polythioether Ancillary Ligands. | The complexes [Ru([9]aneS(3))(dppz)Cl]Cl 1 and [Ru([12]aneS(4))(dppz)]Cl(2)2 ([9]aneS(3) = 1,4,7- trithiaciclononane and [12]aneS(4) = 1,4,7,10-tetrathiaciclododecane) were synthesised and fully characterised. These complexes belong to a small family of dipyridophenazine complexes with non-polypyridyl ancillary ligands... |
18475984 | Construction of Ru(II) Polypyridyl Based Macrocycles: Synthesis, Characterization, Electrochemical, Li Binding, Antitumour and Anti-HIV properties. | Some ruthenium (II) polypyridyl complexes with a bis-chalcone (obtained by the condensation of 3-methyl-thiophene-2-carboxaldehyde and 4-acetyl pyridine) have been synthesized and characterized spectroscopically (IR, NMR, UV/Vis), conductimetric, elemental analysis and FAB mass data. Their luminescent, redox and Li(+) ... |
18475981 | Antibacterial Co(II), Cu(II), Ni(II) and Zn(II) Complexes of Thiadiazoles Schiff Bases. | Schiff bases were obtained by condensation of 2-amino-l,3,4-thiadiazole with 5-substituted-salicylaldehydes which were further used to obtain complexes of the type [M(L)(2)]Cl(2), where M=Co(II), Cu(II), Ni(II) or Zn(II). The new compounds described here have been characterized by physical, spectral and analytical data... |
16233012 | Expanded-bed protein refolding using a solid-phase artificial chaperone. | An efficient solid-phase protein refolding method based on artificial chaperone-assisted refolding is proposed. The method employs insoluble cyclodextrin polymer beads and the expanded-bed technique. Alpha-glucosidase, whose spontaneous refolding yield from a urea-denatured state is up to 30% at a protein concentration... |
16228355 | Identification of the bacteriochlorophyll homologues of Chlorobium phaeobacteroides strain UdG6053 grown at low light intensity. | Detailed APCI LC-MS/MS analysis using an improved HPLC separation reveals the green sulphur bacterium Chlorobium phaeobacteroides strain UdG6053 to contain a wider range of distinct bacteriochlorophyll homologues than has been previously recognised in Chlorobiaceae. The diversity in the homologue distribution is confir... |
11169693 | TADDOLs, Their Derivatives, and TADDOL Analogues: Versatile Chiral Auxiliaries. | TADDOLs, which contain two adjacent diarylhydroxymethyl groups in a trans relationship on a 1,3-dioxolane ring, can be prepared from acetals or ketals of tartrate esters by reaction of the latter with aromatic Grignard reagents. They are extraordinarily versatile chiral auxiliaries. Here, a historical review of the sub... |
11169692 | Dendritic Encapsulation of Function: Applying Nature's Site Isolation Principle from Biomimetics to Materials Science. | The convergence of our understanding of structure-property relationships for selected biological macromolecules and our increased ability to prepare large synthetic macromolecules with a structural precision that approaches that of proteins have spawned a new area of research where chemistry and materials science join ... |
11135410 | Chiral aggregates and asymmetric induction of the reduction of prochiral ketones. | The aim of this work was to establish structure-reactivity relationships between chiral aggregates (micelles, vesicles, and "pseudo-micelles" of amphiphilic dendrimers) and asymmetric induction. In water, micelles or vesicles formed with sugar-headed surfactants gave less than 10% ee in the reduction of prochiral keton... |
12488611 | The Thermal Stability of the Fusarium solani pisi Cutinase as a Function of pH. | We have investigated the thermal stability of the Fusarium solani pisi cutinase as a function of pH, in the range from pH 2-12. Its highest enzymatic activity coincides with the pH-range at which it displays its highest thermal stability. The unfolding of the enzyme as a function of pH was investigated by microcalorime... |
12203651 | A chemosensing ensemble for selective carbonate detection in water based on metal-ligand interactions. | Displacement of the loosely encapsulated fluorescent indicator coumarine 343 (F) from a dicopper(II) cryptate enables the selective fluorimetric detection of the HCO3 (-) ion (A) in water (see scheme, •○=Cu(2+) ). The fluorophore is quenched when encapsulated, but displays its full emission when released to the solutio... |
12203648 | A Hydrogen-Bonded [(Mercuracarborand-Water)2 -Benzene] π-Sandwich Complex. | Two cyclic Lewis acidic receptors, namely B-hexamethyl-9-mecuracarborand-3, [9,12-(CH3 )2 -C2 B10 H8 Hg]3 , each host a single water molecule. A benzene molecule is situated between the two complexed water molecules in a sandwich fashion that suggests there is O-H⋅⋅⋅π hydrogen bonding. |
12203643 | Conjugated complexes composed of quinonediimine and palladium: controlled formation of a conjugated trimetallic macrocycle. | A triangle with a calixarene-like cone configuration: the trimetallamacrocycle [{Pd(en)(L)}3 ](6+) (1; L=N,N'-bis(4-dimethylaminophenyl)-1,4-benzoquinonediimine, en=ethylenediamine; see picture for a space-filling view). The coordination array of the redox-active π-conjugated bridging quinonediimine spacer of L and the... |
12203624 | [2.2]paracyclophane/dehydrobenzoannulene hybrids: transannular delocalization in open-circuited conjugated macrocycles. | Enhanced global delocalization throughout the "stepped" π-electron systems of the [2.2]paracyclophane/dehydrobenzoannulene (PC/DBA) hybrids 1 and 2 is strongly suggested by a comparison of their electronic absorption spectra with those of model compounds with complete and interrupted classical aromatic delocalization. ... |
12203621 | 100 years after grignard: where does the organometallic chemistry of the heavy alkaline Earth metals stand today? | Even a century after the discovery of the Grignard reagents, similar compounds of the heavy alkaline earth metals are still lacking. Owing to the industrial need for such compounds (in particular as anionic polymerization catalysts), the last few years have been marked by a rapid, extensive development which has culmin... |
12203620 | Ring-opened fullerenes: an unprecedented class of ligands for supramolecular chemistry. | A window of opportunity: With the formation of a large orifice in the C60 core (1) and the successful insertion of He and H2 , two critical steps have been realized towards the development of an efficient synthetic approach for the preparation of endohedral fullerene complexes. |
12836335 | Structure/function relationship in the polyplexes containing cationic polypeptides for gene delivery. | Various cationic polypeptides of linear or highly branched structures were synthesized by introducing tertiary or quaternary ammonium groups and hydroxyl groups to poly(L-lysine) (PL) or polyamideamine (PAMAM) dendrimers. These polycations were mixed with plasmid DNA to form polyplexes and subjected to in vitro gene in... |
12836330 | Regulation of aggregated structure of dye molecules by DNA hybridization. | Dye H-aggregates were successfully created by introducing multiple Methyl Red molecules consecutively in the single stranded DNA. By the presence of its complementary DNA, however, the H-band disappeared and new peak appeared at longer wavelength, indicating that aggregated structure changed by the duplex formation. Th... |
12593704 | Neutrophil apoptosis is delayed by trauma patients' plasma via a mechanism involving proinflammatory phospholipids and protein kinase C. | <h4>Background</h4>Delayed apoptosis of primed neutrophils (PMNs) may facilitate PMN-mediated tissue injury leading to multiple organ failure (MOF). We previously reported delayed apoptosis and priming of PMNs in severely injured patients at risk for MOF. Our in vitro and in vivo data have implicated phospholipids in P... |
12549882 | A molecular imprinted polymer with recognition properties towards the carcinogenic mycotoxin ochratoxin A. | A molecularly imprinted polymer which recognises the mycotoxin ochratoxin A was prepared using the mimic N-(4-chloro-1-hydroxy-2-naphthoylamido)-(L)-phenylalanine as a template. The polymer was obtained by dissolving the template, methacrylic acid and ethylendimethacrylate in chloroform and polymerising the mixture by ... |
12549878 | HPLC-based bioseparations using molecularly imprinted polymers. | HPLC-based separations of amino acids and peptides, nucleotide bases, drugs, sugars and steroids using molecularly imprinted polymers (MIPs) have been reviewed in this article. The molecular recognition mechanisms of the template molecules on the MIPs in organic and aqueous eluents were discussed. Furthermore, new poly... |
12549877 | Development of an aspartic acid-based cross-linking monomer for improved bioseparations. | Improved specificity and binding affinity by molecularly imprinted polymers is possible by development of novel functional materials. Furthermore, increasing the cross-link density of imprinted polymers by using cross-linking functional groups was anticipated to improve polymer molecular recognition. A novel cross-link... |
12549870 | Stoichiometric noncovalent interaction in molecular imprinting. | In this review article the function of the binding site monomers in the molecular imprinting procedure is discussed. Especially, new developments towards stoichiometric noncovalent interactions are highlighted. In stoichiometric noncovalent interactions template and binding site monomer in an 1:1 molar ratio are nearly... |
12541838 | [Determination of enantiomeric purity for lactic acid in fermentation broth by Rhizopus oryzae with high performance liquid chromatography]. | A procedure for the resolution of DL-lactic acid and the determination of D-isomer ratio in L-lactic acid fermentation broth by Rhizopus oryzae is described. The effects of pH of mobile phase and concentration of chiral mobile phase additives on resolution of DL-lactic acid were investigated. The optical isomers of lac... |
12541837 | [The effect of temperature on the enantiomeric resolutions on albumin and beta-cyclodextrin chiral stationary phases]. | The effect of column temperature on enantiomeric resolutions of tryptophan, warfarin and ketoprofen was investigated on bovine and human serum albumin (BSA and HSA) stationary phases, which were synthesized with s-triazine as the activator. It was observed that the entropy change made a great contribution to the separa... |
12067451 | Tri-dimensional prostate cell cultures in simulated microgravity and induced changes in lipid second messengers and signal transduction. | The high aspect rotating-wall vessel (HARV) was designed to cultivate cells in an environment that simulate microgravity. We studied previously the effects of HARV cultivation on DU-145 human prostate carcinoma cells. We determined that HARV cultivation produced a less aggressive, slower growing, less proliferative, mo... |
12028841 | Adhesion of MNC to extracellular matrix proteins following in vitro photochemotherapy. | <h4>Background</h4>Photochemotherapy is a safe and effective treatment for patients with drug-resistant severe GvHD. The technique involves the exposure of MNC to psoralen and UVA light (PUVA). We have investigated the effect of in vitro PUVA on MNC adhesion to extracellular matrix (ECM) proteins.<h4>Methods</h4>MNC we... |
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