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15141030 | Influence of loop size on the stability of intramolecular DNA quadruplexes. | We have determined the stability of intramolecular DNA quadruplexes in which the four G(3)-tracts are connected by non-nucleosidic linkers containing propanediol, octanediol or hexaethylene glycol, replacing the TTA loops in the human telomeric repeat sequence. We find that these sequences all fold to form intramolecul... |
15381985 | Bis- and tris-(methylphosphonic) acid derivatives of a 14-membered tetraazamacrocycle containing pyridine: synthesis, protonation and complexation studies. | Two N-methylphosphonic acid derivatives of a 14-membered tetraazamacrocycle containing pyridine have been synthesized, H(4)L(1) and H(6)L(2). The protonation constants of these compounds and the stability constants of complexes of both ligands with Ni(2+), Cu(2+) and Zn(2+) were determined by potentiometric methods at ... |
15136726 | Digitoxin mimics gene therapy with CFTR and suppresses hypersecretion of IL-8 from cystic fibrosis lung epithelial cells. | Cystic fibrosis (CF) is a fatal, autosomal, recessive genetic disease that is characterized by profound lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL-8, and the high levels of IL-8 in the CF lung are therefore believed to be the central ... |
15136724 | Albumin mediates the transcytosis of myeloperoxidase by means of caveolae in endothelial cells. | Myeloperoxidase (MPO), the phagocyte hemoprotein involved in neutrophil host defense and consuming nitric oxide (*NO), induces the nitration of extracellular matrix proteins and tissue remodeling subsequent to its transcytosis across the endothelial barrier. We addressed the role of an interaction of MPO with albumin a... |
15133132 | Clathrin isoform CHC22, a component of neuromuscular and myotendinous junctions, binds sorting nexin 5 and has increased expression during myogenesis and muscle regeneration. | The muscle isoform of clathrin heavy chain, CHC22, has 85% sequence identity to the ubiquitously expressed CHC17, yet its expression pattern and function appear to be distinct from those of well-characterized clathrin-coated vesicles. In mature muscle CHC22 is preferentially concentrated at neuromuscular and myotendino... |
15133125 | Exclusion of lipid rafts and decreased mobility of CD94/NKG2A receptors at the inhibitory NK cell synapse. | CD94/NKG2A is an inhibitory receptor expressed by most human natural killer (NK) cells and a subset of T cells that recognizes human leukocyte antigen E (HLA-E) on potential target cells. To elucidate the cell surface dynamics of CD94/NKG2A receptors, we have expressed CD94/NKG2A-EGFP receptors in the rat basophilic le... |
15133122 | Crystal structures of a DNA octaplex with I-motif of G-quartets and its splitting into two quadruplexes suggest a folding mechanism of eight tandem repeats. | Recent genomic analyses revealed many kinds of tandem repeats of specific sequences. Some of them are related to genetic diseases, but their biological functions and structures are still unknown. Two X-ray structures of a short DNA fragment d(gcGA[G]1Agc) show that four base-intercalated duplexes are assembled to form ... |
15252563 | Polymeric networks of copper(II) using succinate and aromatic N-N donor ligands: synthesis, crystal structure, magnetic behaviour and the effect of weak interactions on their crystal packing. | Four succinato-bridged complexes of copper(II) have been synthesized. Complex 1, [Cu(2)(mu-OH(2))(2)L(bpy)(2)(NO(3))(2)](n) and 2, [Cu(2)(mu-OH(2))(2)L(phen)(2)(NO(3))(2)](n)(bpy = 2,2[prime or minute]-bipyridine; phen = 1,10-phenanthroline and LH(2)= succinic acid) exhibit 1D coordination polymer structures where both... |
15162208 | Calix[4]arenes containing thiourea and amide moieties: neutral receptors towards alpha,omega-dicarboxylate anions. | Two-armed neutral anion receptors (4,5), were prepared and examined for their anion-binding ability using UV-vis, fluorescence and 1H NMR spectra in DMSO. The results of non-linear curve fitting indicate that 4 or 5 form 1 : 1 stoichiometric complexes with dicarboxylate anions by multiple hydrogen bonding interactions ... |
15252569 | Synthesis, structure and electrochemistry of ferrocene-peptide macrocycles. | Redox active cyclopeptides Fc[CSA]2 (5), Fc[Gly-CSA]2 (6), Fc[Ala-CSA]2 (7), Fc[Val-CSA](2) and Fc[Leu-CSA]2 (9) (CSA = cysteamine) which are formed by the reaction of ferrocenedicarboxylic acid with peptide cystamines at high dilutions. These systems exhibit H-bonding involving the amide NH in solution as shown by the... |
15252560 | The structure of phosphine-functionalised silsesquioxane-based dendrimers: a molecular dynamics study. | Molecular dynamics modelling has been used to simulate the structures of phopshine-functionalised, silsesquioxane-based dendrimers whose excellent catalytic properties have been previously demonstrated. The effect of changing the chemical composition of the dendrimer branches is simulated. The results indicate that add... |
15154034 | Synthesis and study of a new diamidodipyrromethane macrocycle. An anion receptor with a high sulfate-to-nitrate binding selectivity. | A new 2,6-diamidopyridinedipyrromethane hybrid macrocycle () has been synthesized that shows a high selectivity for dihydrogen phosphate and hydrogen sulfate relative to nitrate in acetonitrile solution as judged from UV-vis spectrophotometric titrations; this leads to the suggestion that this or related systems might ... |
15123774 | Cross-linking of MHC class I molecules on human NK cells inhibits NK cell function, segregates MHC I from the NK cell synapse, and induces intracellular phosphotyrosines. | Engagement of major histocompatibility complex (MHC) class I molecules on immune cells, where they are usually highly expressed, induces signal transduction events of unclear significance. We show here that antibody-mediated cross-linking of MHC-I molecules on human natural killer (NK) cells inhibits their cytotoxic ac... |
15123801 | The C-terminal domain of DNA gyrase A adopts a DNA-bending beta-pinwheel fold. | DNA gyrase is unique among enzymes for its ability to actively introduce negative supercoils into DNA. This function is mediated in part by the C-terminal domain of its A subunit (GyrA CTD). Here, we report the crystal structure of this approximately 35-kDa domain determined to 1.75-A resolution. The GyrA CTD unexpecte... |
15123800 | Short amino acid stretches can mediate amyloid formation in globular proteins: the Src homology 3 (SH3) case. | Protein misfolding and deposition underlie an increasing number of debilitating human disorders. We have shown that model proteins unrelated to disease, such as the Src homology 3 (SH3) domain of the p58alpha subunit of bovine phosphatidyl-inositol-3'-kinase (PI3-SH3), can be converted in vitro into assemblies with str... |
20803444 | Removal of viruscide agents by using styrenic resins. | Strong and weak anionic polystyrene/divinylbenzene ion exchange resins were investigated both as iodophores and as iodine/iodide ion removal agents in blood disinfection applications. Resin-iodine complexes were prepared, but there was no significant iodine release observed in either distilled water or isotonic saline ... |
18969405 | Ion-transport and diffusion coefficients of non-plasticised methacrylic-acrylic ion-selective membranes. | The ion-transport behaviour of methacrylic-acrylic-based polymers for ion-selective electrode (ISE) membranes was investigated by a spectrophotometric method to determine the apparent diffusion coefficient. By observing the degree of deprotonation of the chromoionophore or chromogenic ionophore, the extent of penetrati... |
18950092 | Giant multiporphyrin arrays as artificial light-harvesting antennas. | Synthetic giant multiporphyrin arrays with well-defined architectures are reviewed in terms of artificial light-harvesting materials. Meso,meso-linked porphyrin arrays and multiporphyrin dendrimers have successfully mimicked the light-harvesting function of bacterial photosynthetic systems. We have also developed novel... |
18950110 | Insights into the Structure of Large-Ring Cyclodextrins through Molecular Dynamics Simulations in Solution. | Molecular dynamics simulations were carried out using the AMBER parm99 force field and explicit water molecules (TIP3P) to gain insight into the structural deformations and energetics of several large-ring cyclodextrins (with a degree of polymerization 26, 30, 55, 70, 85, and 100) in solution. The structures displayed ... |
18950109 | Self-assembly and characterization of a novel hydrogen-bonded nanostructure. | A novel hydrogen-bonded supramolecular system of a [60]fullerene derivative with perylene bisimide was synthesized and characterized. 1H NMR spectra confirmed the existence of strong hydrogen-bonding interaction between compounds 1 and 5. Transmission electron microscopy images of 1.5 aggregates showed spherical partic... |
18429251 | Overview of protein structural and functional folds. | This overview provides an illustrated, comprehensive survey of some commonly observed protein-fold families and structural motifs, chosen for their functional significance. It opens with descriptions and definitions of the various elements of protein structure and associated terminology. Following is an introduction in... |
17147616 | Reduction of starch granule size by expression of an engineered tandem starch-binding domain in potato plants. | Granule size is an important parameter when using starch in industrial applications. An artificial tandem repeat of a family 20 starch-binding domain (SBD2) was engineered by two copies of the SBD derived from Bacillus circulans cyclodextrin glycosyltransferase via the Pro-Thr-rich linker peptide from Xyn10A from Cellu... |
15981921 | Antitumor activity of beta-cyclodextrin polymer-camptothecin conjugates. | Antitumor activity of linear, beta-cyclodextrin polymer (CDP)-camptothecin (CPT) conjugates (HGGG6, LGGG10, HG6, and HGGG10) is investigated in nude mice bearing human LS174T colon carcinoma tumors. These conjugates differ in polymer molecular mass [97 kDa (H) or 35 kDa (L)], CDP-CPT linker structure [glycine (G) or tr... |
15969416 | Langmuir-Blodgett films of amphiphilic bis(tetrathiafulvalene) macrocycles with four alkyl chains. | Amphiphilic bis(tetrathiafulvalene) [bis(TTF)] macrocycles with four alkyl chains were fabricated as novel electrically active Langmuir-Blodgett (LB) films. Two TTF units were linked via [24]crown-8, [21]crown-7, and [18]crown-6 macrocycles, forming charge-transfer (CT) salts with 2,3,5,6-tetrafluoro-7,7,8,8-tetracyano... |
15969412 | Nanoscale probing of the enamel nanorod surface using polyamidoamine dendrimers. | Although it is known that noncollagenous proteins of dental origin bind to the hydroxyapatite crystal surfaces, no measure of their binding strength has been calculated. This experiment used -COOH-capped generation 7 PAMAM dendrimers as nanoprobes of the biological hydroxyapatite nanorod surfaces. Dendrimer distributio... |
15969388 | Self-organization of self-assembled tetrameric porphyrin arrays on surfaces. | The incorporation of designed self-assembled supramolecular structures into devices requires deposition onto surfaces with retention of both structure and function. This remains a challenge and can present a significant barrier to developing devices using self-organizing materials. To examine the role of peripheral gro... |
15969371 | Partitioning of macrocyclic compounds in a cationic and an anionic micellar solution: a small-angle neutron scattering study. | Following a previous investigation on partitioning of some macrocycle compounds in sodium dodecyl sulfate (SDS) and dodecyltrimethylammonium bromide (DTAB) aqueous solutions and their effect on the micellar structure, a small-angle neutron scattering (SANS) study has been performed at fixed surfactant content (0.20 mol... |
15969364 | Highly fluorescent streptavidin-coated CdSe nanoparticles: preparation in water, characterization, and micropatterning. | CdSe quantum dots (QDs) with a high fluorescence quantum yield of 25% and a narrow size distribution were synthesized in a single step in water using glutathione as a stabilizing molecule. The exceptional optical properties enabled for the first time the detection of in-water-prepared single quantum dots at room temper... |
15969189 | Amine-terminated dendrimers as biomimetic templates for silica nanosphere formation. | In diatoms, silica synthesis occurs by use of complex posttranslationally modified peptides, termed silaffins, and highly complex biological polyamine structures. Silaffin peptides have lysine residues that are modified to long-chain polyamine moieties of N-methyl derivatives of polypropylenimine to drive silica synthe... |
15969152 | Unified model to predict self-assembly of nonionic surfactants in solution and adsorption on solid or fluid hydrophobic surfaces: effect of molecular structure. | We have developed a pseudo-phase model to predict the self-assembly of nonionic surfactants on hydrophobic solid or fluid interfaces and in bulk solution. The uniqueness of this model is that it provides the relationship between molecular structure and self-assembly in solution and on interfaces. This model requires th... |
15969151 | Experimental observations on the scaling of adsorption isotherms for nonionic surfactants at a hydrophobic solid-water interface. | The self-assembly of nonionic surfactants in bulk solution and on hydrophobic surfaces is driven by the same intermolecular interactions, yet their relationship is not clear. While there are abundant experimental and theoretical studies for self-assembly in bulk solution and at the air-water interface, there are only f... |
15969148 | Walljet electrochemistry: quantifying molecular transport through metallopolymeric and zirconium phosphonate assembled porphyrin square thin films. | By employing redox-active probes, condensed-phase molecular transport through nanoporous thin films can often be measured electrochemically. Certain kinds of electrode materials (e.g. conductive glass) are difficult to fabricate as rotatable disks or as ultramicroelectrodes-the configurations most often used for electr... |
15769054 | [Synthesis of 2-(4-dimethylaminophenyl)-5-fluoro-6-(morpholin-4-yl)-1H-benzimidazole and its spectroscopic research]. | A new potential pharmaceutical compound, 2-(4-dimethylaminophenyl)-5-fluoro-6-(morpholin-4-yl)-1H-benzimidazole (4) was synthesized. The effect of the pH value of aqueous solution on the absorption spectrum of compound 4 and its interaction with beta-cyclodextrin (beta-CD) were investigated by UV-Vis spectrum, steady-s... |
15712919 | [Chiral separation of 3-phenyllactic acid by capillary electrophoresis]. | Various factors affecting the chiral resolution of 3-phenyllactic acid by capillary zone electrophoresis such as the type of cyclodextrin, concentration of the chiral selector, buffer pH, voltage, and temperature were studied and optimized. Using 30 mmol/L hydroxypropyl-beta-cyclodextrin as the chiral selector, and 0.1... |
15629016 | High perfomance liquid chromatography in pharmaceutical analyses. | In testing the pre-sale procedure the marketing of drugs and their control in the last ten years, high performance liquid chromatography replaced numerous spectroscopic methods and gas chromatography in the quantitative and qualitative analysis. In the first period of HPLC application it was thought that it would becom... |
15595458 | Reverse-docking as a computational tool for the study of asymmetric organocatalysis. | A novel methodology for 'reverse-docking' a cationic peptide-based organocatalyst to a rigid anionic transition state (TS) model for the conjugate addition of azide to alpha,beta-unsaturated carbonyl substrates is described. The resulting docking poses serve as simplified TS models for enantioselective catalysis. Molec... |
15370963 | Influence of different recombinant systems on the cooperativity exhibited by cytochrome P4503A4. | 1. The in vitro cooperativity exhibited by cytochrome P450 (CYP) 3A4 is influenced by the nature of the recombinant system in which the phenomenon is studied. Diclofenac, piroxicam and R-warfarin were used as model substrates, and quinidine was the effector. 2. The 5-, 5'- and 10-hydroxylation of diclofenac, piroxicam ... |
15344664 | [Aromatic crown ethers as phase transfer catalysts in the synthesis of N-acetylglucosamine beta-aryl glycosides]. | The crown ether-catalyzed glycosylation of phenol, 4-methoxyphenol, and 4-nitrophenol was studied under phase transfer conditions in solid-liquid system. The asymmetric dibenzocrown esters are superior to [3.3]dibenzo-1 8-crown-6 and 15-crown-5 in the catalysis of these reactions. The English version of the paper: Russ... |
15267864 | Two-dimensional Monte Carlo simulations of ionic and nonionic silane self-assembly on hydrophilic surfaces. | Aqueous chemistries have recently been shown to be useful for the deposition of hydrophobic films of nonionic and cationic silanes on hydrophilic substrates for the prevention of stiction in MEMS. The Monte Carlo method is used to simulate in two dimensions the self-assembly of silane films on a hydrophilic surface. We... |
15267830 | Determination of noncovalent interaction energies from electronic densities. | Starting from the Hellmann-Feynman theorem, an expression is derived for the interaction energy in forming a noncovalently bound complex. It invokes only classical electrostatics, involving the charge distributions of the components as they are in the complex. We propose a method for obtaining these from the total char... |
15267745 | Amplification of chirality in helical supramolecular polymers beyond the long-chain limit. | The optical activity of helical homopolymers devoid of chiral centers increases drastically when a small amount of homochiral monomers is incorporated into them. We study this so-called sergeants-and-soldiers effect of chirality amplification in solutions of helical supramolecular polymers with a theoretical model that... |
15253453 | Suffocation of nerve fibers by living nanovesicles: a model simulation. | A model using nanospheres to allow the simulation of the nonspecific interaction of nanobacteria (NB), one with another or with body tissues, is established. Depending primarily on their concentrations and stress levels, these apatite nanovesicles may nucleate thrombogenic conglomerates in blood, or self-assemble to de... |
15253425 | Proteome analysis of lipid rafts in Jurkat cells characterizes a raft subset that is involved in NF-kappaB activation. | Lipid rafts are detergent-insoluble membrane domains that play a key role in signal transduction by the T-cell antigen receptor. Proteome analysis revealed the presence of amidosulfobetaine-soluble signal transducing, integral membrane, cytoskeletal, heat shock, and GTP-binding proteins in rafts prepared from Jurkat ce... |
15214684 | Determination and regulation of the migration window in electrokinetic chromatography. | The determination of the velocities of the mobile and the pseudostationary phases (the migration (time) window) is mandatory for the determination of physicochemical properties by electrokinetic chromatography (EKC). This review offers a detailed discussion on the definition, the importance, the determination and the r... |
15176882 | In situ and Ex situ adsorption and recovery of betalains from hairy root cultures of Beta vulgaris. | Various adsorbents were screened for in situ recovery of betalain pigments effluxed from hairy root cultures of red beet, Beta vulgaris. Alumina/silica (1:1) appeared ideal, showing in situ adsorption of 97% in a unit time of 30 min accounting for in situ recovery of 71.39% of the total betalaine effluxed. Other adsorb... |
15174039 | Single-isomer sulfated alpha-cyclodextrins for capillary electrophoresis. Part 2. Hexakis(6-O-sulfo)-alpha-cyclodextrin: synthesis, analytical characterization, and initial screening tests. | The second member of the family of single-isomer sulfated alpha-cyclodextrins, the sodium salt of hexakis(6-O-sulfo)-alpha-cyclodextrin (HxS), has been synthesized, analytically characterized, and used as the resolving agent for the capillary electrophoretic separation of the enantiomers of nonionic, weak-acid and weak... |
15174038 | Chiral cyclodextrin-modified microemulsion electrokinetic chromatography. | Cyclodextrin (CD)-modified microemulsion electrokinetic chromatography (MEEKC) or CD-MEEKC has not previously been applied to the area of chiral separations. Herein, the results of investigations of various microemulsions with CD additives are presented. Two different microemulsions are explored: an ethyl acetate sodiu... |
15166136 | In vivo analysis of synaptonemal complex formation during yeast meiosis. | During meiotic prophase a synaptonemal complex (SC) forms between each pair of homologous chromosomes and is believed to be involved in regulating recombination. Studies on SCs usually destroy nuclear architecture, making it impossible to examine the relationship of these structures to the rest of the nucleus. In Sacch... |
15162386 | Encapsulation of isohexenylnaphthazarins in cyclodextrins. | Naturally occurring isohexenylnaphthazarins (IHN), such as Alkannin, Shikonin (A/S) and their derivatives, are potent pharmaceutical substances with a wide spectrum of biological activity. In the present study, inclusion complexes of alkannin and shikonin commercial samples and IHN derivatives in the form of an oily ex... |
15157911 | Effect of binary and ternary polyethyleneglycol and/or beta-cyclodextrin complexes on the photochemical and photosensitizing properties of Naproxen. | The effect of the polyethylene glycol and/or beta-cyclodextrin on the photolability of aqueous solutions of the anti-inflammatory drug Naproxen was studied. In all systems studied, the photodegradation process followed zero-order kinetics, leading to the same photoproducts as in the absence of these additives. Kinetic ... |
15157908 | Investigation on DNA assembly to neutral red-cyclodextrin complex by molecular spectroscopy. | DNA assembling to neutral red (NR) and cyclodextrins (CDs)-NR inclusion complex has been investigated by means of absorption, fluorescence and resonance light scattering (RLS). Depending on the molar ratio R of NR:DNA, the binding of NR with DNA involved in two processes at pH 7.50 and ionic strength 0.0045. The first ... |
15159455 | The sequences appended to the amyloid core region of the HET-s prion protein determine higher-order aggregate organization in vivo. | The [Het-s] prion of the fungus Podospora anserina propagates as a self-perpetuating amyloid form of the HET-s protein. This protein triggers a cell death reaction termed heterokaryon incompatibility when interacting with the HET-S protein, an allelic variant of HET-s. HET-s displays two distinct domains, a N-terminal ... |
15149195 | An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology. | The bacterial cell surface layer (S-layer) protein of Bacillus sphaericus CCM 2177 assembles into a square lattice structure and recognizes a distinct type of secondary cell wall polymer (SCWP) as the proper anchoring structure in the rigid cell wall layer. For generating a nanopatterned sensing layer with high density... |
15147805 | Continuous release of rh-interferon alpha-2a from triglyceride matrices. | The use of biodegradable polymeric matrices as controlled release systems is known to be associated with various drawbacks. The objective of this study was to develop an alternative delivery system based on triglycerides, thereby aiming for sustained continuous protein release. Tristearin implants containing lyophilise... |
15146911 | Advanced mesoporous organosilica material containing microporous beta-cyclodextrins for the removal of humic acid from water. | A new mesoporous organosilica material (beta-CD-Silica-4%) containing microporous beta-cyclodextrins (beta-CDs) has been prepared by the co-polymerization of a silylated beta-CD monomer with tetraethoxysilane in the presence of a structure-directing template, cetyltrimethylammonium bromide. Solid-state 13C and 29Si NMR... |
15146910 | Development of new solid-phase microextraction fibers by sol-gel technology for the determination of organophosphorus pesticide multiresidues in food. | Allyloxy bisbenzo 16-crown-5 trimethoxysilane was first used as precursor to prepare the sol-gel-derived bisbenzo crown ether/hydroxyl-terminated silicone oil (OH-TSO) SPME coating. The coating procedure involving sol solution composition and conditioning process was presented. Compared with commercial SPME stationary ... |
15146527 | Molecular shuttles based on tetrathiafulvalene units and 1,5-dioxynaphthalene ring systems. | Six different degenerate [2]rotaxanes were synthesized and characterized. The rotaxanes contained either two tetrathiafulvalene (TTF) units or two 1,5-dioxynaphthalene (DNP) ring systems, both of which serve as recognition sites for a cyclobis(paraquat-p-phenylene) (CBPQT4+) ring. Three different spacer units were inco... |
15146515 | Kinetic precipitation of solution-phase polyoxomolybdate followed by transmission electron microscopy: a window to solution-phase nanostructure. | This study aimed to elucidate the structural nature of the polydisperse, nanoscopic components in the solution and the solid states of partially reduced polyoxomolybdate derived from the [Mo132] keplerate, [(Mo)Mo5]12-[Mo2 acetate]30. Designer tripodal hexamine-tris-crown ethers and nanoscopic molybdate coprecipitated ... |
15146513 | Electronic and vibrational properties of fluorenone in the channels of zeolite L. | Fluorenone (C13H8O) was inserted into the channels of zeolite L by using gas-phase adsorption. The size, structure, and stability of fluorenone are well suited for studying host-guest interactions. The Fourier transform IR, Raman, luminescence, and excitation spectra, in addition to thermal analysis data, of fluorenone... |
15137036 | Solid-phase biotinylation of antibodies. | Biotinylation is an established method of labeling antibody molecules for several applications in life science research. Antibody functional groups such as amines, cis hydroxyls in carbohydrates or sulfhydryls may be modified with a variety of biotinylation reagents. Solution-based biotinylation is accomplished by incu... |
15137035 | A new method for the screening of solid-phase combinatorial libraries for affinity chromatography. | A new methodology for the rapid assessment of affinity ligands synthesized by combinatorial solid-phase chemistry is reported. This screening strategy utilizes the target protein conjugated to FITC, and represents an almost universal technique for the preliminary screening of solid-phase combinatorial libraries. The as... |
15137034 | The design, synthesis and evaluation of affinity ligands for prion proteins. | Bifunctional affinity ligands based on a triazine scaffold were rationally designed to target prion protein and shown to bind recombinant prion protein with high affinity and selectivity. The ligands were capable of discriminating between prion protein glycoforms and monomeric and dimeric forms of the prion protein. Th... |
15137033 | Is there a rational method to purify proteins? From expert systems to proteomics. | The purification of recombinant proteins for therapeutic or analytical applications requires the use of several chromatographic steps in order to achieve a high level of purity. A range of techniques is available such as anion and cation exchange chromatography, which can be carried out at different pHs, and hence used... |
15137032 | An artificial receptor for glycoproteins. | We describe the rational design, synthesis and development of a sterilizable biomimetic ligand for the affinity purification of glycoproteins. Based on mimicking the principles of natural carbohydrate recognition, a putative library of 196 glycoprotein-binding synthetic ligands was designed and synthesized on a polymer... |
15137031 | Unique interaction of scorpion toxins with the hERG channel. | ERG potassium channels specify one component of the delayed rectifier in the heart and are likely to play an important functional role in other excitable cells. Compared to other K+ channels, the human ERG (hERG) channel possesses an unusually long S5-P linker that presumably forms an alpha-helix important for channel ... |
15137030 | Proteins, recognition networks and developing interfaces for macromolecular biosensing. | Genomics and proteomics discovery is leading to the identification of all proteins and to the opportunity, and challenge, to reveal the protein recognition networks that drive virtually all biological processes. Over the past decade, biosensors have emerged as a key technology for detection and analysis of biomolecular... |
15137029 | Molecular tools for a molecular medicine: analyzing genes, transcripts and proteins using padlock and proximity probes. | Procedures and reagents are needed to specifically detect all the macromolecules that are being identified in the course of genome projects. We discuss how this challenge may be met using a set of ligation-based reagents termed padlock probes and proximity ligation probes. These probes include elements with affinity fo... |
15137028 | On-chip single-cell-based microcultivation method for analysis of genetic information and epigenetic correlation of cells. | We have developed an on-chip single-cell based microcultivation method for analyzing the variability of genetic information stored in single cells and their epigenetic correlations. The method uses four systems: an on-chip cell sorter for purifying the cells in a non-destructive manner; an on-chip single-cell cultivati... |
15137027 | Optimisation of polymeric surface pre-treatment to prevent bacterial biofilm formation for use in microfluidics. | The production of a microfluidic device for microbial culture has necessitated the development of techniques for the prevention of bacterial adhesion to a range of polymeric substrates including fluoropolymers such as fluorinated ethylene polypropylene, and polyolefins such as low-density polyethylene. Treatment of suc... |
15137026 | The application of the acoustic spectrophonometer to biomolecular spectrometry: a step towards acoustic "fingerprinting". | A tunable acoustic biosensor for investigating the properties of biomolecules at the solid-liquid interfaces is described. In its current, format the device can be tuned to frequencies between 6.5 MHz and 1.1 GHz in order to provide a unique detection feature: a variable evanescent wave thickness at the sensor surface.... |
15137025 | The tunnelling conductance of molecularly ordered metalloprotein arrays. | Metalloproteins can be self-assembled in molecularly ordered, electrochemically addressable arrays. We report here on a study of the transport characteristics of the blue copper protein, azurin, from Pseudomonas aeruginosa, by a combination of electrochemical and scanning probe techniques (scanning tunnelling microscop... |
15137024 | Glucose-sensitive holographic sensors. | Holographic sensors for monitoring glucose were fabricated from hydrogel films containing chemical ligands based on phenylboronic acid. The films were transformed into reflection holograms using a diffusion method coupled with exposure to laser light. The diffraction wavelength of the holograms was used to monitor the ... |
15137023 | SPR for molecular interaction analysis: a review of emerging application areas. | PubMed searches identified four emerging application areas for surface plasmon resonance systems. Food analysis, proteomics, immunogenicity and drug discovery. These application areas are reviewed. In connection with the review of drug discovery applications a case study is presented. This study demonstrates the value ... |
15134923 | Zn-, Cd-, and Pb-transcription factor IIIA: properties, DNA binding, and comparison with TFIIIA-finger 3 metal complexes. | Properties of the metal ion binding sites of Zn-transcription factor IIIA (TFIIIA) were investigated to understand the potential of this type of zinc finger to undergo reactions that remove Zn(2+) from the protein. Zn-TFIIIA was purified from E. coli containing the cloned sequence for Xenopus laevis oocyte TFIIIA and i... |
15134916 | Configurations of metallocyclams and relevance to anti-HIV activity. | Antiviral cyclam macrocycles block viral entry into cells by binding to the CXCR4 co-receptor. Cyclams bind transition metal ions strongly and can potentially form a range of trans (I-V) and cis configurations which may be recognised differently by co-receptor proteins. A survey of the CSD (crystallographic structural ... |
15134912 | Novel gadolinium(III) polyaminocarboxylate macrocyclic complexes as potential magnetic resonance imaging contrast agents. | Two novel Gd(III) complexes with functionalised polyaminocarboxylate macrocycles, 1,4,7-tris(carboxymethyl)-9,24-dioxo-14,19-dioxa-1,4,7,10,23- pentaazacyclododecane (L(1)) and 1,4,7-tris(carboxymethyl)-9,25-dioxo-14,17,20-trioxa-1,4,7,10,23- pentaazacyclotridecane (L(2)), were prepared in good yield. Their potential u... |
15132675 | Thermodynamic studies on the adsorption of fibronectin adhesion-promoting peptide on nanothin films of poly(2-vinylpyridine) by SPR. | This paper describes a methodology for preparing uniform, nanothin polymer films for the study of biomolecule adsorption by surface plasmon resonance spectroscopy (SPR). The methodology combines molecular self-assembly of alkanethiols on gold with surface-confined atom transfer radical polymerization (ATRP). Poly(2-vin... |
15132657 | Phase behavior and hydration of silk fibroin. | The osmotic stress method was applied to study the thermodynamics of supramolecular self-assembly phenomena in crystallizable segments of Bombyx mori silkworm silk fibroin. By controlling compositions and phases of silk fibroin solution, the method provided a means for the direct investigation of microscopic and thermo... |
15128250 | Thiabowls: synthesis, molecular structure, and novel supramolecular architecture of trithia-[3]-peristylane. | A synthesis of trithia-[3]-peristylane, C(6)H(6)S(3), a novel C(3)(v)() symmetric thiabowl, from the exotic hydrocarbon bullvalene has been accomplished. The X-ray crystal structure of this trithiabowl displays an unprecedented supramolecular architecture in the solid state with 12 CH.S interactions involving all of it... |
15120897 | Cyclodextrins as a potential carrier in drug nebulization. | The inhalation route is widely studied for many drug applications focusing on either local or systemic distributions. One matter of concern is the solubilization of hydrophobic drugs. We have studied the feasibility of using different cyclodextrins (CDs) to elaborate pharmaceutical formulations for the inhalation route... |
15117209 | HPLC and MALDI-TOF MS analysis of highly branched polystyrene: resolution enhancement by branching. | Temperature gradient interaction chromatography (TGIC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were applied for the characterization of highly branched polystyrenes (PS) prepared by linking living polystyryl anions using 4-chlorodimethylsilylstyrene. Reversed-phas... |
15114052 | Gene expression profiling: from microarrays to medicine. | With the mapping of the human genome comes the ability to identify genes of interest in specific diseases and the pathways involved therein. Laboratory technology has evolved in parallel, providing us with the ability to assay thousands of these genes at once, a technique known as microarray analysis. The main question... |
15113675 | Synthesis of 6I-amino-6I-deoxy-2I-VII,3I-VII-tetradeca-O-methyl-cyclomaltoheptaose. | The preparation of 6(I)-amino-6(I)-deoxy-2(I-VII),3(I-VII)-tetradeca-O-methyl-cyclomaltoheptaose is reported. Two different routes (A and B), both starting from beta-cyclodextrin (betaCD), have been examined. Route A involved: (i) synthesis of heptakis(6-O-tert-butyldimethylsilyl)-betaCD from betaCD; (ii) permethylatio... |
15113668 | Synthesis, self-assembly and characterization of a new glucoside-type hydrogel having a Schiff base on the aglycon. | A new hydrogel based on a substituted phenyl glucoside with a Schiff base in the aglycon was synthesized, and the self-assembling characteristics was studied. FTIR spectra, UV-vis absorption spectra and X-ray diffraction (XRD) revealed that pi-pi interactions between the Schiff base moieties, hydrogen bonds, and the in... |
15113664 | Regioselective monoacylation of cyclomaltoheptaose at the C-2 secondary hydroxyl groups by the alkaline protease from Bacillus subtilis in nonaqueous media. | Transesterification of cyclomaltoheptaose (beta-CD) with divinyl butanedioate, divinyl hexanedioate, and divinyl decanedioate, catalyzed by the alkaline protease from Bacillus subtilis in anhydrous DMF for 5 days, furnished the corresponding vinyl-beta-CD derivatives. The products were characterized by ESI-MS, (1)H NMR... |
15111647 | Regulation of sodium channel function by bilayer elasticity: the importance of hydrophobic coupling. Effects of Micelle-forming amphiphiles and cholesterol. | Membrane proteins are regulated by the lipid bilayer composition. Specific lipid-protein interactions rarely are involved, which suggests that the regulation is due to changes in some general bilayer property (or properties). The hydrophobic coupling between a membrane-spanning protein and the surrounding bilayer means... |
15111444 | Cytomechanical properties of papaver pollen tubes are altered after self-incompatibility challenge. | Self-incompatibility (SI) in Papaver rhoeas triggers a ligand-mediated signal transduction cascade, resulting in the inhibition of incompatible pollen tube growth. Using a cytomechanical approach we have demonstrated that dramatic changes to the mechanical properties of incompatible pollen tubes are stimulated by SI in... |
15111442 | Specific recognition of macroscopic objects by the cell surface: evidence for a receptor density threshold revealed by micrometric particle binding characteristics. | The establishment of specific molecular bonds between a cell and a facing surface is involved in many physiological and technological situations. Using micrometric magnetic particles, we have explored the formation of specific molecular bonds between the cell and surfaces bearing complementary ligands under passive con... |
15111440 | Dynamic elastic modulus of porcine articular cartilage determined at two different levels of tissue organization by indentation-type atomic force microscopy. | Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was developed to measure the dynamic elastic modulus, |E(*)|, in compression by indentation-type atomic force microscopy (IT AF... |
15111434 | Substrate-dependent morphology of supramolecular assemblies: fibrillin and type-VI collagen microfibrils. | Substrate hydrophobicity/hydrophilicity has previously been shown to affect the morphology and biological function of isolated proteins. We have employed atomic force microscopy to investigate substrate dependent morphologies of two biochemically distinct native supramolecular assemblies: fibrillin and type-VI collagen... |
15111433 | Theoretical analysis of twist/bend ratio and mechanical moduli of bacterial flagellar hook and filament. | Certain motile bacteria employ rotating flagella for propulsion. The relative flexibility of two key components of the flagellum, filament and hook, is partially responsible for the mechanistic workings of this motor. A new computational method, the quantized elastic deformational model, was employed in this article to... |
15111432 | Mechanism of accelerated assembly of beta-amyloid filaments into fibrils by KLVFFK(6). | Extracellular senile plaques are a central pathological feature of Alzheimer's disease. At the core of these plaques are fibrillar deposits of beta-amyloid peptide (Abeta). In vitro, Abeta spontaneously assembles into amyloid fibrils of cross-beta sheet structure. Although it was once believed that the fibrils themselv... |
15111431 | Force spectroscopy of collagen fibers to investigate their mechanical properties and structural organization. | Tendons are composed of collagen and other molecules in a highly organized hierarchical assembly, leading to extraordinary mechanical properties. To probe the cross-links on the lower level of organization, we used a cantilever to pull substructures out of the assembly. Advanced force probe technology, using small cant... |
15111423 | Insight into the structural role of carotenoids in the photosystem I: a quantum chemical analysis. | The structural stabilization role of carotenoids in the formation of photosynthetic pigment-protein complexes is investigated theoretically. The pi-pi stacking and CH-pi interactions between beta-carotenes and their surrounding chlorophylls (and/or aromatic residues) in Photosystem I (PS1) from the cyanobacterium Synec... |
15111417 | Myosin regulatory domain orientation in skeletal muscle fibers: application of novel electron paramagnetic resonance spectral decomposition and molecular modeling methods. | Reorientation of the regulatory domain of the myosin head is a feature of all current models of force generation in muscle. We have determined the orientation of the myosin regulatory light chain (RLC) using a spin-label bound rigidly and stereospecifically to the single Cys-154 of a mutant skeletal isoform. Labeled RL... |
15111410 | Nonequilibrium behavior in supported lipid membranes containing cholesterol. | We investigate lateral organization of lipid domains in vesicles versus supported membranes and monolayers. The lipid mixtures used are predominantly DOPC/DPPC/Chol and DOPC/BSM/Chol, which have been previously shown to produce coexisting liquid phases in vesicles and monolayers. In a monolayer at an air-water interfac... |
15111390 | Size effects on diffusion processes within agarose gels. | To investigate diffusion processes in agarose gel, nanoparticles with sizes in the range between 1 and 140 nm have been tested by means of fluorescence correlation spectroscopy. Understanding the diffusion properties in agarose gels is interesting, because such gels are good models for microbial biofilms and cells cyto... |
15110839 | S1 subsite in snake venom thrombin-like enzymes: can S1 subsite lipophilicity be used to sort binding affinities of trypsin-like enzymes to small-molecule inhibitors? | Thrombin-like enzymes isolated from snake venoms comprise a group of serine proteinases responsible for many important coagulation disorders in the envenomed victims. Besides, these proteinases have great biotechnological interest as antithrombotic agents and as diagnostic tools. However, in spite of the recent overflo... |
15100179 | Influence of phenylalanines 77 and 138 on the stereospecificity of aryl sulfotransferase IV. | Aryl sulfotransferase (AST) IV (also named tyrosine-ester sulfotransferase and ST1A1) is a major phenol sulfotransferase in the rat, and it catalyzes the sulfation of many drugs, carcinogens, and other xenobiotics that contain phenol, benzylic alcohol, N-hydroxy arylamine, and oxime functional groups. Previous work dis... |
15096634 | Proteins can convert to beta-sheet in single crystals. | Raman microscopy was used to follow conformational changes in single protein crystals. Crystals of native insulin and of the 5S and 12S subunits of the enzyme transcarboxylase showed a mixture of Raman marker bands signifying alpha-helix, beta-sheet and nonordered secondary structure. However, by reducing the S-S bonds... |
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