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15060283 | Asymmetric autocatalysis: novel structures, novel mechanism? | The amplifying asymmetric autocatalysis discovered by Soai and coworkers in 1995 does not fit easily into the extensively investigated framework of organozinc alkylation of aldehydes. In that case the catalyst is a monomeric Zn chelate that functions as both Lewis acid and Lewis base, permitting the simultaneous coordi... |
15099109 | Quantitative evaluation of the catalytic activity of dendrimers with only one active center at the core: application to the nitroaldol (Henry) reaction. | One reference tertiary amine and three families of structurally related trialkylamines and dendrimers have been synthesized, characterized, and studied by molecular dynamics simulations. The catalytic activity of these amines in the nitroaldol (Henry) reaction between 2-nitroethanol and benzaldehyde has been measured b... |
15099079 | Formation of inclusion organoactinide complexes with boron-containing macrocycles. | Reaction of the organoactinide complexes (C5Me5)2AnMe2 (An = Th, U) with catecholborane yields an inclusion complex where the actinide is encapsulated inside a 15-membered, hexaoxo, trianionic macrocycle built from alternating catechol and catecholborate fragments. In the presence of LiOH, a dimer of two encapsulated a... |
15100044 | Resonance Raman characterization of the P intermediate in the reaction of bovine cytochrome c oxidase. | Reduced cytochrome c oxidase binds molecular oxygen, yielding an oxygenated intermediate first (Oxy) and then converts it to water via the reaction intermediates of P, F, and O in the order of appearance. We have determined the iron-oxygen stretching frequencies for all the intermediates by using time-resolved resonanc... |
15098799 | Influence of grapefruit juice on the systemic availability of itraconazole oral solution in healthy adult volunteers. | <h4>Study objective</h4>To evaluate the effect of repeated ingestion of grapefruit juice on the systemic availability of itraconazole (ITZ) and hydroxyitraconazole (OHITZ) serum concentrations in subjects administered hydroxypropyl-beta-cyclodextrin-ITZ (HP-beta-CD ITZ) oral solution.<h4>Design</h4>Randomized, two-peri... |
15089628 | Anomalous temperature dependence of surfactant self-assembly from aqueous solution. | The self-assembly of surfactant molecules from aqueous solutions exhibits anomalous and as yet unexplained temperature dependence. These effects include (a) a decrease in surfactant solubility, (b) an increase of the critical micelle concentration, and (c) increasing self-assembly on solid surfaces with increasing temp... |
15093656 | Prevention of experimental diffuse lamellar keratitis using a novel platelet-activating factor receptor antagonist. | <h4>Purpose</h4>To determine whether a novel platelet-activating factor (PAF) antagonist prevents experimentally induced diffuse lamellar keratitis (DLK) after laser in situ keratomileusis (LASIK).<h4>Settings</h4>Department of Ophthalmology and Neuroscience Center of Excellence, Louisiana State University Health Scien... |
15094200 | Novel plastid gene minicircles in the dinoflagellate Amphidinium operculatum. | Seven new minicircles, forming part of the fragmented plastid genome of the dinoflagellate Amphidinium operculatum, have been identified by PCR. Three minicircles are full-length, one of 2.6 kb encoding the 23S rRNA gene, one of 2.4 kb containing the psaB gene, and a third of 2.5 kb containing the psbD, psbE and psbI g... |
15095018 | Investigation of the effects of copper ions on protein aggregation using a model system. | Protein aggregation is a notable feature of various human disorders, including Parkinson's disease, Alzheimer's disease and many others systemic amyloidoses. An increasing number of observations in vitro suggest that transition metals are able to accelerate the aggregation process of several proteins found in pathologi... |
15095012 | MAGUKs in synapse assembly and function: an emerging view. | Neuronal morphogenesis, synaptogenesis and synaptic plasticity are fundamental aspects of nervous system development. Much of our current understanding of how each of these processes contributes to the establishment and maintenance of neural circuitry has come from a molecular description of specific classes of key mol... |
15095007 | Snake venom thrombin-like enzymes: from reptilase to now. | The snake venom thrombin-like enzymes (SVTLEs) comprise a number of serine proteases functionally and structurally related to thrombin. Until recently, only nine complete sequences of this subgroup of the serine protease family were known. Over the past 5 years, the primary structure of several SVTLEs has been characte... |
15088757 | Chiral separation of highly negatively charged enantiomers by capillary electrophoresis. | The separation of two highly negatively charged enantiomeric organic disulfates containing two chiral centers was investigated by capillary electrophoresis using cyclodextrin based chiral selectors added to the run buffer. The optimum separation for the enantiomers was achieved in less than 3 min at 25 degrees C with a... |
15087183 | Mass transfer and bioremediation of naphthalene particles in a roller bioreactor. | Naphthalene particles in a water slurry have been bioremediated in a sealed, roller bioreactor using a pure strain of Pseudomonas putida. High stripping losses of particles due to both splashing and aeration made the use of the traditional CSTR bioreactor unsuitable for bioremediation of naphthalene particles. The over... |
15086495 | Micro-MRI methods to detect renal cysts in mice. | <h4>Background</h4>Mouse models of disease, especially using transgenic and knockout technologies, are powerful tools to analyze the molecular basis of disease. We recently reported that a new dynamic micro-MRI method with dendrimer-based contrast agents can visualize renal structure and function in normal living mice ... |
15084663 | Lipid rafts and integrin activation regulate oligodendrocyte survival. | Newly formed oligodendrocytes in the CNS derive survival cues from their target axons. These cues are provided in part by laminins expressed on the axon, which are recognized by alpha6beta1 integrin on the oligdendrocyte and amplify platelet-derived growth factor (PDGF) signaling through the phosphatidylinositol 3'-kin... |
15081813 | Core and heterogeneity of beta2-microglobulin amyloid fibrils as revealed by H/D exchange. | Dialysis-related amyloidosis, which occurs in the patients receiving a long-term hemodialysis with high frequency, accompanies the deposition of amyloid fibrils composed of beta(2)-microglobulin (beta2-m). In vitro, beta2-m forms two kinds of fibrous structures at acidic pH. One is a rigid "mature fibril", and the othe... |
15081172 | Solubilization of the neutral and charged forms of 2,4,6-trichlorophenol by hydroxypropyl-beta-cyclodextrin, or methyl-beta-cyclodextrin in water. | The solubility of 2,4,6-trichlorophenol (TCP) in hydroxypropyl-beta-cyclodextrin (HPbetaCD), or methyl-beta-cyclodextrin (MebetaCD) at pH 3.0 and 8.8 reported by Hanna et al. [J. Hazard. Mater. B100 (2003) 109] is reevaluated in this paper. The complexations of the individual dissolved forms were examined. As expected,... |
15080687 | Alpha-turn mimetics: short peptide alpha-helices composed of cyclic metallopentapeptide modules. | Alpha-Helices are key structural components of proteins and important recognition motifs in biology. Short peptides (<or=15 residues) corresponding to these helical sequences are rarely helical away from their stabilizing protein environments. New techniques for stabilizing short peptide helices could be valuable for s... |
15080681 | Fabrication of superhydrophobic surface from a supramolecular organosilane with quadruple hydrogen bonding. | A supramolecular organosilane, 2-(3-(triethoxysilyl)propylaminocarbonylamino)-6-methyl-4[1H]pyrimidinone comprising quadruple hydrogen bonds has been synthesized in one step from commercially available starting materials. The synthesized supramolecular organosilane can be stabilized and phase-separated by dimerization ... |
15078478 | Use of the molecular adsorbent recycling system in the treatment of postoperative hepatic failure and septic multiple organ dysfunction--preliminary results. | <h4>Purpose of the study</h4>Molecular adsorbent recycling system (MARS) has been applied successfully in patients with a variety of liver diseases. However, preliminary results in patients with multiple organ failure (MOF) and sepsis were disappointing, possibly because the number of applied MARS cycles was too low. T... |
15078902 | Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer. | fEndosome-to-Golgi retrieval of the mannose 6-phosphate receptor (MPR) is required for lysosome biogenesis. Currently, this pathway is poorly understood. Analyses in yeast identified a complex of proteins called "retromer" that is essential for endosome-to-Golgi retrieval of the carboxypeptidase Y receptor Vps10p. Retr... |
15078223 | Physical mechanisms and biological significance of supramolecular protein self-assembly. | In living cells, chemical reactions of metabolism, information processing, growth and development are organized in a complex network of interactions. At least in part, the organization of this network is accomplished as a result of physical assembly by supramolecular scaffolds. Indeed, most proteins function in cells w... |
15078154 | DNA measurements by using fluorescence correlation spectroscopy and two-color fluorescence cross correlation spectroscopy. | FCS and FCCS measurements provide two important analytical parameters, the average number of molecules in the detection area and the translational diffusion constant of the molecules at the single molecule level. Considering these properties, FCS and FCCS have been applied to analysis of the cellular environment and dy... |
15078158 | Aspartate and glutamate mimetic structures in biologically active compounds. | Glutamate and aspartate are frequently recognized as key structural elements for the biological activity of natural peptides and synthetic compounds. The acidic side-chain functionality of both the amino acids provides the basis for the ionic interaction and subsequent molecular recognition by specific receptor sites t... |
15079844 | Multiple recognition of barbiturate guests by Hamilton-receptor-functionalized dendrimers. | The well-known unsubstituted "Hamilton receptor" was monofunctionalized with an amino group and attached at the periphery of poly(propyleneamine) dendrimers through the use of an activated ester. Four generations of Hamilton-receptor-functionalized dendrimers (HR-dendrimers) were synthesized and characterized by (1)H a... |
15079842 | The structure of metallomicelles. | The morphology of micelles formed by two novel metallosurfactants has been studied by small-angle neutron scattering (SANS) and small-angle-X-ray scattering (SAXS). The two surfactants both contain a dodecyl chain as the hydrophobic moiety, but differ in the structure of the head group. The surfactants are Cu(II) compl... |
15079834 | Supramolecular recognition: protonmotive-driven switches or motors? | A dicationic molecular receptor bearing two cofacially disposed terpyridyl-Pd-Cl units forms stable 1:1 host-guest complexes with planar, neutral platinum(II) complexes. When the guest is modified to incorporate a pyridine group, the now basic guest is protonated by trifluoroacetic acid in acetonitrile solutions. The b... |
15079832 | A mechanically interlocked bundle. | The prototype of an artificial molecular machine consisting of a trisammonium tricationic component interlocked with a tris(crown ether) component to form a molecular bundle with averaged C(3v) symmetry has been designed and synthesized. The system is based on noncovalent interactions, which include 1) N(+)-H...O hydro... |
15079830 | Towards surface-supported supramolecular architectures: tailored coordination assembly of 1,4-benzenedicarboxylate and Fe on Cu(100). | We present a comprehensive investigation of the modular assembly of surface-supported metal-organic coordination systems with specific topologies and high structural stability formed by vapor deposition of 1,4-benzenedicarboxylic acid molecules and iron atoms on a Cu(100) surface under ultra-high vacuum conditions. By ... |
15079829 | A neutral organometallic fluoro complex can be a good ligand. | The reaction of the complex [Mo(OTf)(eta(3)-C(3)H(4)-Me-2)(CO)(2)(phen)] (1) (OTf = trifluoromethylsulfonate; phen = 1,10-phenanthroline) with tetrabutylammonium fluoride trihydrate afforded the fluoride complex [MoF(eta(3)-C(3)H(4)-Me-2)(CO)(2)(phen)] (2). The IR spectrum and the oxidation potential of 2 reflect the f... |
15079826 | Residual topological isomerism of intertwined molecules. | The growing number of molecular assemblies with unusual geometry and topology requires from time to time a revision of certain aspects of stereochemistry. The present paper analyzes several representatives of intertwined molecules that have bridges connecting their loops. In spite of the experimentally proven chirality... |
15079825 | Structure-switching signaling aptamers: transducing molecular recognition into fluorescence signaling. | The development of aptamer technology considerably broadens the utility of nucleic acids as molecular recognition elements, because it allows the creation of DNA or RNA molecules for binding a wide variety of analytes (targets) with high affinity and specificity. Several recent studies have focused on developing ration... |
15074954 | Incorporation of a flexible, pyridine-functionalized Diaza-crown ether into discrete supramolecules via coordination-driven self-assembly. | A flexible, pyridine-functionalized diaza-crown ether was self-assembled into discrete supramolecules of differing stoichiometries upon combination with various organoplatinum molecules. They are characterized by electrospray ionization mass spectrometry and (31)P[(1)H] and (1)H NMR. In one case, (1)H-(1)H NOE enhancem... |
15074989 | [C6N2H14]0.5.[MnAl3(PO4)4(H2O)2]: a manganese(II)-substituted aluminophosphate with AFN topology. | A new manganese(II)-substituted aluminophosphate, [C(6)N(2)H(14)]0.5.[MnAl(3)(PO(4))(4)(H(2)O)(2)], denoted as MnAPO-14, has been synthesized hydrothermally in the presence of 1,4-diazabicyclo[2.2.2]octane (DABCO) as the structure-directing agent. Its structure is determined by single-crystal X-ray diffraction analysis... |
15073374 | Reversible optical transcription of supramolecular chirality into molecular chirality. | In nature, key molecular processes such as communication, replication, and enzyme catalysis all rely on a delicate balance between molecular and supramolecular chirality. Here we report the design, synthesis, and operation of a reversible, photoresponsive, self-assembling molecular system in which molecular and supramo... |
15071026 | Molecular analysis of adenovirus isolates from vaccinated and unvaccinated young adults. | Infections of adenovirus type 4 (Ad4) and Ad7 were discovered among previously vaccinated individuals through febrile respiratory illness surveillance at military recruit camps. Genetic analysis was performed on these isolates and a sample of adenovirus isolates from unvaccinated patients. Antigenic regions of the aden... |
15070355 | Anion-pi interactions in a carousel copper(II)-triazine complex. | Unprecedented anion-pi interactions are revealed for the electron-poor triazine rings in [L2(CuCl)3][CuCl4]Cl (L = hexakis(pyridin-2-yl)-[1,3,5]-triazine-2,4,6-triamin), where both the chloride ion and a Cl atom of [CuCl4]2- are located approximately 3.15 A above the ring centroids, in excellent agreement with theoreti... |
15070351 | Halogen bonding and pi...pi stacking control reactivity in the solid state. | The halogen bonding and the pi...pi stacking interactions induce the noncovalent self-assembly of modules into photoreactive supramolecular architecture. The pi...pi interaction pre-organizes the template, and the halogen bonding aligns the olefins to conform to the topochemical principle for photoreaction. The UV irra... |
15070339 | Highly polarized luminescence from optical quality films of a semiconducting polymer aligned within oriented mesoporous silica. | In this Communication, we show that nanometer scale control of semiconducting polymer chain conformation is possible using host/guest chemistry in highly ordered and macroscopically oriented thin films of mesoporous silica. This control leads to a thin film composite material that is optically transparent, densely fill... |
15069723 | Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of cagA gene, CagA protein in Helicobacter pylori isolates and its antibody in sera of patients. | <h4>Aim</h4>To construct a prokaryotic expression system of a Helicobacter pylori (H pylori) cagA gene fragment and establish enzyme-linked immunosorbent assays (ELISA) for detecting CagA and its antibody, so as to understand the manner in which the infection of CagA-expressing H pylori (CagA(+) H pylori) isolates caus... |
15066429 | Structural basis for telomeric single-stranded DNA recognition by yeast Cdc13. | The essential budding yeast telomere-binding protein Cdc13 is required for telomere replication and end protection. Cdc13 specifically binds telomeric, single-stranded DNA (ssDNA) 3' overhangs with high affinity using an OB-fold domain. We have determined the high-resolution solution structure of the Cdc13 DNA-binding ... |
15066425 | High pressure promotes circularly shaped insulin amyloid. | Amyloids, initially associated with certain degenerative diseases, and recently with the prions and prion-based inheritance in yeasts, are linearly-ordered beta-sheet-rich protein aggregates, presently thought to represent a rather common generic trait of proteins as polymers. Regardless of genetic origins and properti... |
15067042 | Cutting edge: optical microspectrophotometry supports the existence of gel phase lipid rafts at the lamellipodium of neutrophils: apparent role in calcium signaling. | Although much progress has been made in elucidating the biochemical properties of lipid rafts, there has been less success in identifying these structures within living cell membranes, which has led to some concern regarding their existence. One difficulty in analyzing lipid rafts using optical microscopy is their smal... |
15065861 | Long-chain fatty acid uptake into adipocytes depends on lipid raft function. | This study investigates the role of lipid rafts and caveolae, a subclass of lipid raft microdomains, in the binding and uptake of long-chain fatty acids (LCFA) by 3T3-L1 cells during differentiation. Disruption of lipid rafts by beta-cyclodextrin (betaCD) or selective inhibition of caveolae by overexpression of a domin... |
15066816 | Identification of Streptococcus thermophilus CNRZ368 genes involved in defense against superoxide stress. | To better understand the defense mechanism of Streptococcus thermophilus against superoxide stress, molecular analysis of 10 menadione-sensitive mutants, obtained by insertional mutagenesis, was undertaken. This analysis allowed the identification of 10 genes that, with respect to their putative functions, were classif... |
15063120 | Unique physicochemical properties of human enteric Ad41 responsible for its survival and replication in the gastrointestinal tract. | Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is n... |
15063756 | Polyamidoamine dendrimers inhibit binding of Tat peptide to TAR RNA. | The binding of polyamidoamine (PAMAM) dendrimer or Tat peptide to trans-acting responsive element (TAR) RNA has been studied using microgravimetric quartz crystal microbalance (QCM). Experimental results showed that PAMAM dendrimer could form complexes with TAR RNA. Especially, PAMAM dendrimer could disrupt the interac... |
15064155 | Screening for estrogen and androgen receptor activities in 200 pesticides by in vitro reporter gene assays using Chinese hamster ovary cells. | We tested 200 pesticides, including some of their isomers and metabolites, for agonism and antagonism to two human estrogen receptor (hER) subtypes, hERalpha and hERbeta, and a human androgen receptor (hAR) by highly sensitive transactivation assays using Chinese hamster ovary cells. The test compounds were classified ... |
15065269 | A perspective of one-pot pyrrole-aldehyde condensations as versatile self-assembly processes. | Regarded as the classic one-pot synthetic route to symmetrical porphyrins for well over half a century, pyrrole-aldehyde cyclocondensations have yielded a cornucopia of nonporphyrin macrocycles, such as N-confused porphyrins, corroles, sapphyrins, and expanded porphyrins, and have thus emerged as versatile self-assembl... |
15060178 | Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy. | A small container of several to a few hundred microm3 (i.e. bacterial cells and eukaryotic nuclei) contains extremely long genomic DNA (i.e. mm and m long, respectively) in a highly organized fashion. To understand how such genomic architecture could be achieved, Escherichia coli nucleoids were subjected to structural ... |
15060148 | pVHL modification by NEDD8 is required for fibronectin matrix assembly and suppression of tumor development. | Functional inactivation of the von Hippel-Lindau (VHL) tumor suppressor gene is the cause of the familial VHL disease and most sporadic renal clear-cell carcinomas (RCC). pVHL has been shown to play a role in the destruction of hypoxia-inducible factor alpha (HIF-alpha) subunits via ubiquitin-mediated proteolysis and i... |
15060043 | Genetic analysis of the Salmonella enterica type III secretion-associated ATPase InvC defines discrete functional domains. | An essential component of all type III secretion systems is a highly conserved ATPase that shares significant amino acid sequence similarity to the beta subunit of the F(0)F(1) ATPases and is thought to provide the energy for the secretion process. We have performed a genetic and functional analysis of InvC, the ATPase... |
15060032 | The Tp38 (TpMglB-2) lipoprotein binds glucose in a manner consistent with receptor function in Treponema pallidum. | A 38-kDa lipoprotein of Treponema pallidum (Tp38) was predicted to be a periplasmic sugar-binding protein based on its sequence similarity to the glucose/galactose-binding (MglB) protein of Escherichia coli (P. S. Becker, D. R. Akins, J. D. Radolf, and M. V. Norgard, Infect. Immun. 62:1381-1391, 1994). Inasmuch as gluc... |
14725506 | A functional chimaeric S-layer-enhanced green fluorescent protein to follow the uptake of S-layer-coated liposomes into eukaryotic cells. | The chimaeric gene encoding a C-terminally truncated form of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 and the EGFP (enhanced green fluorescent protein) was ligated into plasmid pET28a and cloned and expressed in Escherichia coli. Just 1 h after induction of expression an intense EGFP fluorescence was de... |
14720122 | Characterization of iron binding in IscA, an ancient iron-sulphur cluster assembly protein. | Iron-sulphur clusters are one of the most common types of redox centre in biology. At least six proteins (IscS, IscU, IscA, HscB, HscA and ferredoxin) have been identified as being essential for the biogenesis of iron-sulphur proteins in bacteria. It has been shown that IscS is a cysteine desulphurase that provides sul... |
14717658 | Heparan sulphate proteoglycans modulate fibroblast growth factor-2 binding through a lipid raft-mediated mechanism. | We investigated how lipid raft association of HSPG (heparan sulphate proteoglycans) modulates FGF-2 (fibroblast growth factor-2/basic fibroblast growth factor) interactions with vascular smooth-muscle cells. When lipid rafts were disrupted with sterol-binding agents, methyl-beta-cyclodextrin and filipin, FGF-2 binding ... |
14678011 | Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophy. | Although the primary abnormality in dystrophin is the underlying cause for mdx (X-chromosome-linked muscular dystrophy), abnormal Ca2+ handling after sarcolemmal microrupturing appears to be the pathophysiological mechanism leading to muscle weakness. To develop novel pharmacological strategies for eliminating Ca2+-dep... |
15053624 | Combining magnetic resonance spectroscopies, mass spectrometry, and molecular dynamics: investigation of chiral recognition by 2,6-di-O-methyl-beta-cyclodextrin. | EPR spectroscopy has been employed to investigate the formation of complexes between heptakis-(2,6-O-dimethyl)-beta-cyclodextrin (DM-beta-CD) and different enantiomeric pairs of chiral nitroxides of general structure PhCH2NO.CH(R)R'. Accurate equilibrium measurements of the concentrations of free and included radicals ... |
15053616 | Toward synthetic tubes for NO2/N2O4: design, synthesis, and host-guest chemistry. | Design of molecular nanotubes is proposed for entrapment and conversion of NO2/N2O4 gases. Synthesis of 1,3-alternate bis-calix[4]arene tube 3 of 5 x 11 A internal dimensions is presented, and its reversible reactions with NO2/N2O4 in solution are studied. Exposure of 3 to NO2/N2O4 in chlorinated solvents results in th... |
15053610 | Quantitative vibrational dynamics of iron in nitrosyl porphyrins. | We use quantitative experimental and theoretical approaches to characterize the vibrational dynamics of the Fe atom in porphyrins designed to model heme protein active sites. Nuclear resonance vibrational spectroscopy (NRVS) yields frequencies, amplitudes, and directions for 57Fe vibrations in a series of ferrous nitro... |
15053608 | Design of a functional protein for molecular recognition: specificity of ligand binding in a metal-assembled protein cavity probed by 19f NMR. | A metal-assembled homotrimeric coiled coil based on the GCN4-p1 sequence has been designed that noncovalently binds hexafluorobenzene and other similar ligands in a hydrophobic cavity, created by making the core substitution Asn16Ala ([Fe(bpyGCN4-N16A)3]2+). The KD of binding of hexafluorobenzene with [Fe(bpyGCN4-N16A)... |
15053584 | Redox-active star molecules incorporating the 4-benzoylpyridinium cation: implications for the charge transfer efficiency along branches vs across the perimeter in dendrimers. | We report the redox properties of four star systems incorporating the 4-benzoyl-N-alkylpyridinium cation; the redox potential varies along the branches but remains constant at fixed radii. Bulk electrolysis shows that at a semi-infinite time scale all redox centers are electrochemically accessible. However, voltammetri... |
15053575 | Characterization of tectoRNA assembly with cationic conjugated polymers. | Association between RNAs with preprogrammed molecular recognition units can be quantified by using cationic, water-soluble conjugated polymers. The method uses a fluorophore-labeled probe RNA (RNA-F*), which is treated with a target structure (RNA-T). Heterodimer formation, (RNA-T/RNA-F*), increases the total negative ... |
15053572 | Nanohybrids from liquid crystalline extended amphiphilic dendrimers. | A novel extended amphiphilic dendrimer with linear poly(ethylene oxide) (PEO) attached to a PEO-like dendritic core as hydrophilic fraction and eight docosyl chain branches as hydrophobic fraction has been prepared for the use as structure-directing agent for silica-type materials. The extended dendrimer exhibits a hex... |
15053565 | Supraphos: a supramolecular strategy to prepare bidentate ligands. | We report a new strategy for the preparation of chelating bidentate ligands, which involves just the mixing of two monodentate ligands functionalized with complementary binding sites. In the current example, the assembly process is based on selective metal-ligand interactions, using phosphite zinc(II) porphyrins 1-6 an... |
15053548 | Enantioselective analysis of free and glycosidically bound monoterpene polyols in Vitis vinifera L. cvs. Morio Muscat and Muscat Ottonel: evidence for an oxidative monoterpene metabolism in grapes. | The enantiomeric ratios of various free and glycosidically bound monoterpene polyols in musts of the aromatic grapes Vitis vinifera L. cvs. Morio Muscat and Muscat Ottonel were determined by means of enantioselective multidimensional gas chromatography-mass spectrometry. Reference compounds of defined stereochemistry w... |
15053547 | Effect of beta-cyclodextrin on aroma release and flavor perception. | Binding and release of volatile compounds to and from beta-cyclodextrin were measured in model aqueous systems using static equilibrium headspace and dynamic headspace dilution. Beta-cyclodextrin decreased the static equilibrium headspace for some volatiles (e.g., ethyl octanoate and decanone) due to binding, but dilut... |
15052679 | Construction of a prokaryotic expression system of vacA gene and detection of vacA gene, VacA protein in Helicobacter pylori isolates and ant-VacA antibody in patients' sera. | <h4>Aim</h4>To construct a recombinant prokaryotic expression vector inserted with Helicobacter pylori vacA gene and identify the immunity of the expressed recombinant protein, and to determine prevalence of vacA-carrying/VacA expressing H pylori isolates and seroprevalence of specific ant-VacA antibody in H pylori inf... |
15052678 | Construction of prokaryotic expression system of ureB gene from a clinical Helicobacter pylori strain and identification of the recombinant protein immunity. | <h4>Aim</h4>To clone ureB gene from a clinical isolate of Helicobacter pylori and construct a prokaryotic expression system of the gene and identify immunity of the expressed recombinant protein.<h4>Methods</h4>ureB gene from a clinical H pylori strain Y06 was amplified by the high fidelity polymerase chain reaction te... |
15049810 | Control of Cre recombination by regulatory elements from Xer recombination systems. | Site-specific recombination by the Cre recombinase takes place at a simple DNA site (loxP), requires no additional proteins and gives topologically simple recombination products. In contrast, cer and psi sites for Xer recombination contain approximately 150 bp of accessory sequences, require accessory proteins PepA, Ar... |
15049674 | Self-assembly of interlocked supramolecular dendrimers. | Interlocked supramolecular dendrimers were spontaneously self-assembled from molecular components, metallocycles, and dumbbells bearing benzyl ether repeating units. Here, the metallocycles were in situ self-assembled from L-shaped ligands with dendritic branches, 2,3-dimethyl-2-butene and osmium tetraoxide. The supram... |
15049654 | Synthesis of a pondaplin dimer and trimer. Aromatic interactions in novel macrocycles. | Synthetic challenges in the use of an oxabicyclo[2.2.2]octenone moiety as a masked arene for the synthesis of pondaplin are disclosed. During the course of a study of the Heck reaction as a tool for macrocyclization to provide strained paracyclophanes, novel macrocycles displaying intra- and intermolecular aromatic int... |
15050829 | Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p. | The Saccharomyces cerevisiae homologue of the linker histone H1, Hho1p, has two domains that are similar in sequence to the globular domain of H1 (and variants such as H5). It is an open question whether both domains are functional and whether they play similar structural roles. Preliminary structural studies showed th... |
15050290 | Effects of methyl-beta-cyclodextrin on T lymphocytes lipid rafts with aging. | Aging is associated with a decline in immune functions. Among them, T-cell activation is altered at several points of the signaling cascade following TCR engagement. Recent findings suggest that lipid rafts act as a platform in the initiation of T-cell activation. We have previously demonstrated that cholesterol conten... |
15051494 | CD44 interaction with ankyrin and IP3 receptor in lipid rafts promotes hyaluronan-mediated Ca2+ signaling leading to nitric oxide production and endothelial cell adhesion and proliferation. | In this study, we have showed that aortic endothelial cells (GM7372A cell line) express CD44v10 [a hyaluronan (HA) receptor], which is significantly enriched in cholesterol-containing lipid rafts (characterized as caveolin-rich plasma membrane microdomains). HA binding to CD44v10 promotes recruitment of the cytoskeleta... |
15047828 | CD4-independent infection of astrocytes by human immunodeficiency virus type 1: requirement for the human mannose receptor. | Human immunodeficiency virus type 1 (HIV-1) infection occurs in the central nervous system and causes a variety of neurobehavioral and neuropathological disorders. Both microglia, the residential macrophages in the brain, and astrocytes are susceptible to HIV-1 infection. Unlike microglia that express and utilize CD4 a... |
15047825 | Human immunodeficiency virus type 1 Nef activates p21-activated kinase via recruitment into lipid rafts. | The Nef protein of human immunodeficiency virus type 1 is an important factor in AIDS pathogenesis. In addition to downregulating CD4 and major histocompatibility complex class I molecules from the cell surface, as well as increasing virion infectivity, Nef triggers activation of the T-cell receptor (TCR) cascade to fa... |
15046982 | Distinct properties of the two putative "globular domains" of the yeast linker histone, Hho1p. | The putative linker histone in Saccharomyces cerevisiae, Hho1p, has two regions of sequence (GI and GII) that are homologous to the single globular domains of linker histones H1 and H5 in higher eukaryotes. However, the two Hho1p "domains" differ with respect to the conservation of basic residues corresponding to the t... |
15047066 | Selective molecular recognition of arginine by anionic salt bridge formation with bis-phosphate crown ethers: implications for gas phase peptide acidity from adduct dissociation. | Arginine forms a stable noncovalent anionic salt bridge complex with DP (a crown ether which contains two endocyclic dialkylhydrogenphosphate esters). Abundant adduct formation with DP is observed for complexes with arginine, YAKR, HPPGFSPFR, AAKRKAA, RR, RPPGFSPFR, RYLGYL, RGDS, and YGGFMRGL in electrospray ionization... |
15046504 | Self-assembly of lanthanide helicate coordination polymers into 3D metal-organic framework structures. | Pyridine-2,6-dicarboxylic acid (pdcH(2) ) reacts with nitrate salts of La(III), Ce(III), and Nd(III) under hydrothermal conditions to form three-dimensional network structures, 1-4. All the compounds crystallize in the monoclinic space group P2(1)/c with the following lattice parameters: [La(2)(pdc)(3).3H(2)O] 1, a = 1... |
15046501 | Systematic preparation of Mo 2 4+ building blocks for supramolecular assemblies. | The preparation of additional and useful building blocks for the construction of supramolecular entities with quadruply bonded Mo(2)(4+) units has been explored, and five new mixed-ligand complexes with three types of ligands and various basicities are reported. The ligands used were the DAniF (N,N'-di-p-anisylformamid... |
15046491 | Syntheses, structural characterization, and host-guest chemistry of ethynylcrown ether containing polynuclear gold(I) complexes. | Two novel ethynylcrown ether containing di- and tetranuclear gold(I) complexes have been synthesized and structurally characterized; their binding ability toward various metal ions has also been studied. |
15047186 | Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers. | Heterologous expression of CYP73A5, an Arabidopsis cytochrome P450 monooxygenase, in baculovirus-infected insect cells yields correctly configured P450 detectable by reduced CO spectral analysis in microsomes and cell lysates. Co-expression of a housefly NADPH P450 reductase substantially increases the ability of this ... |
15047533 | Relationship between antiviral activity and host toxicity: comparison of the incorporation efficiencies of 2',3'-dideoxy-5-fluoro-3'-thiacytidine-triphosphate analogs by human immunodeficiency virus type 1 reverse transcriptase and human mitochondrial DNA polymerase. | Emtricitabine [(-)FTC; (-)-beta-L-2'-3'-dideoxy-5-fluoro-3'-thiacytidine] is an oxathiolane nucleoside analog recently approved by the Food and Drug Administration for the treatment of human immunodeficiency virus (HIV). Structurally, (-)FTC closely resembles lamivudine [(-)3TC] except that the former is 5-fluorinated ... |
15047519 | Efficacy, safety, and plasma pharmacokinetics of escalating dosages of intravenously administered ravuconazole lysine phosphoester for treatment of experimental pulmonary aspergillosis in persistently neutropenic rabbits. | Ravuconazole is a new antifungal triazole with broad-spectrum activity and a long half-life in plasma. We studied the antifungal efficacy, safety, and pharmacokinetics of ravuconazole lysine phosphoester in escalating dosages for the treatment of invasive pulmonary aspergillosis due to Aspergillus fumigatus in persiste... |
15047507 | Interaction of the new ketolide ABT-773 (cethromycin) with human polymorphonuclear neutrophils and the phagocytic cell line PLB-985 in vitro. | A classical velocity centrifugation technique was used to study the in vitro uptake of the new ketolide ABT-773 by human polymorphonuclear neutrophils (PMNs) and a myelomonoblastic cell line, PLB-985, which can be differentiated into PMNs under certain culture conditions, compared to that of HMR 3004. ABT-773 was rapid... |
15044735 | Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements. | The variation in inhibitor specificity for five different amine inhibitors bound to CST, BT, and the cold-adapted AST has been studied by use of association constant measurements, structural analysis of high-resolution crystal structures, and the LIE method. Experimental data show that AST binds the 1BZA and 2BEA inhib... |
15044732 | Conformational mapping of the N-terminal peptide of HIV-1 gp41 in lipid detergent and aqueous environments using 13C-enhanced Fourier transform infrared spectroscopy. | The N-terminal domain of HIV-1 glycoprotein 41,000 (gp41) participates in viral fusion processes. Here, we use physical and computational methodologies to examine the secondary structure of a peptide based on the N terminus (FP; residues 1-23) in aqueous and detergent environments. (12)C-Fourier transform infrared (FTI... |
15040742 | Enantiodivergent synthesis of both enantiomers of marine alkaloids haliclorensin and isohaliclorensin, a constituent of halitulin. | Starting from (3R)-5-benzotriazolyl-3-phenylperhydropyrido[2,1-b][1,3]oxazole 9, the enantiodivergent syntheses of both enantiomers of the marine alkaloids haliclorensin 1 and isohaliclorensin 3 have been achieved. Our syntheses feature ring-expansion reactions for the formation of the aza-macrocycle ring system of 3 a... |
15040731 | Helical chirality in donor-acceptor catenanes. | A [2]catenane in which the macrocyclic polyether, bisparaphenylene[34]crown-10, is interlocked with the tetracationic cyclophane, cyclobis(paraquat-p-phenylene), is shown by dynamic (1)H NMR spectroscopy, using (i). neutral and (ii). anionic chiral shift reagents (CSRs), to exist at low temperatures (197 K) in acetone-... |
15040730 | p-(1H-phenanthro[9,10-d]imidazol-2-yl)- substituted calix[4]arene, a deep cavity for guest inclusion. | The reaction of tetra-p-formyltetra-O-propylcalix[4]arene with phenanthrenequinone in the presence of NH(4)OAc affords compound 2, a new class of calixarene with an expanded aromatic cavity, that could be stabilized by hydrogen-bonded bridges and/or ion pairing, thus preventing collapse into fully stacked pinched cone ... |
15040729 | Structure and photoluminescence of a benzil nanocolumn in a C-methylcalix[4]resorcinarene-based framework. | A new framework based on C-methylcalix[4]resorcinarene and the flexible nonconjugated spacer 1,4-bis(imidazol-1yl-methyl)benzene encloses a large one-dimensional channel, containing benzil nanocolumns. Unlike in a previously reported series of benzil-containing supramolecular solids with conjugated linker molecules, be... |
15040727 | Versatile formation of [2]catenane and [2]pseudorotaxane structures; threading and noncovalent stoppering by a self-assembled macrocycle. | A self-assembled dimeric macrocycle between 4,4'-bis(4-pyridylmethoxy)biphenyl (L) and (en)Pd(NO(3))(2) was constructed, and its interactions with cyclodextrins of different cavity size resulted in the formation of [2]catenane and [2]pseudorotaxane systems, respectively. The structures were identified by 1D and 2D NMR ... |
15040726 | Controllable, selective per-functionalization of dendritic oligoamines. | A method allowing for an unprecedented controllable functionalization of oligoamines via N,N-bis-sulfonylation with various sulfonyl chlorides has been developed. Depending on the nature of the sufonyl chloride and reaction conditions such as base, time of reaction, and temperature, each amino group can be selectively ... |
15040725 | A novel colorimetric and fluorescent anion chemosensor based on the flavone quasi-crown ether-metal complex. | The metal-ligand complex 1 ([Mg (L)] (2+)) (or 2 ([Ca (L)]( 2+))) was demonstrated to selectively bind HSO(4)(-) (or H(2)PO(4)(-)) over other anions by using UV-vis absorption and fluorescence spectroscopy. The studied complex exhibits the remarkable color change and fluorescence quenching upon introducing HSO(4)(-) (o... |
15041685 | Self-association process of a peptide in solution: from beta-sheet filaments to large embedded nanotubes. | Lanreotide is a synthetic octapeptide used in the therapy against acromegaly. When mixed with pure water at 10% (w/w), Lanreotide (acetate salt) forms liquid crystalline and monodisperse nanotubes with a radius of 120 A. The molecular and supramolecular organization of these structures has been determined in a previous... |
15041680 | Force spectroscopy of the double-tethered concanavalin-A mannose bond. | We present the measurement of the force required to rupture a single protein-sugar bond using a methodology that provides selective discrimination between specific and nonspecific binding events and helps verify the presence of a single functional molecule on the atomic force microscopy tip. In particular, the interact... |
15041678 | Detection and characterization of partially unfolded oligomers of the SH3 domain of alpha-spectrin. | For the purpose of equilibrium and kinetic folding-unfolding studies, the SH3 domain of alpha-spectrin (spc-SH3) has long been considered a classic two-state folding protein. In this work we have indeed observed that the thermal unfolding curves of spc-SH3 measured at pH 3.0 by differential scanning calorimetry, circul... |
15041674 | In vitro self-assembly of the light harvesting pigment-protein LH2 revealed by ultrafast spectroscopy and electron microscopy. | Controlled ensemble formation of protein-surfactant systems provides a fundamental concept for the realization of nanoscale devices with self-organizing capability. In this context, spectroscopic monitoring of pigment-containing proteins yields detailed structural information. Here we have studied the association behav... |
15041649 | Membrane model for the G-protein-coupled receptor rhodopsin: hydrophobic interface and dynamical structure. | Rhodopsin is the only member of the pharmacologically important superfamily of G-protein-coupled receptors with a known structure at atomic resolution. A molecular dynamics model of rhodopsin in a POPC phospholipid bilayer was simulated for 15 ns, revealing a conformation significantly different from the recent crystal... |
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