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15116210 | Towards configurationally stable bisindolylmaleimide cyclophanes: potential tools for investigating protein kinase function. | The effect of macrocycle size and substitution on the configurational stability of some bisindolylmaleimide cyclophanes was determined. |
15064363 | Ded1p, a conserved DExD/H-box translation factor, can promote yeast L-A virus negative-strand RNA synthesis in vitro. | Viruses are intracellular parasites that must use the host machinery to multiply. Identification of the host factors that perform essential functions in viral replication is thus of crucial importance to the understanding of virus-host interactions. Here we describe Ded1p, a highly conserved DExD/H-box translation fact... |
15064359 | Energetic basis for selective recognition of T*G mismatched base pairs in DNA by imidazole-rich polyamides. | To complement available structure and binding results and to develop a detailed understanding of the basis for selective molecular recognition of T.G mismatches in DNA by imidazole containing polyamides, a full thermodynamic profile for formation of the T.G-polyamide complex has been determined. The amide-linked hetero... |
22833693 | Northern Ring Conformation of Methanocarba-Adenosine 5'-Triphosphate Required for Activation of P2X Receptors. | Strategy, Management and Health PolicyEnabling Technology, Genomics, ProteomicsPreclinical ResearchPreclinical Development Toxicology, Formulation Drug Delivery, PharmacokineticsClinical Development Phases I-III Regulatory, Quality, ManufacturingPostmarketing Phase IVReplacement of the ribose moiety of adenosine 5'-tri... |
19475004 | Bloch method for the analysis of modes in microstructured optical fibers. | We discuss a transform technique for analyzing the wave vector content of microstructured optical fiber (MOF) modes, which is computationally efficient and gives good physical insight into the nature of the mode. In particular, if the mode undergoes a transition from a bound state to an extended state, this is evident ... |
18969391 | Chiral separation of enantiomers of amino acid derivatives by high-performance liquid chromatography on a norvancomycin-bonded chiral stationary phase. | A novel norvancomycin-bonded chiral stationary phase (NVC-CSP) was synthesized by using the chiral selector of norvancomycin. The chiral separation of enantiomers of several dansyl-amino acids by high-performance liquid chromatography (HPLC) in the reversed-phase mode is described. The effects of some parameters, such ... |
18969380 | Preparation of dendrimer-like polyamidoamine immobilized silica gel and its application to online preconcentration and separation palladium prior to FAAS determination. | A amino-terminated G 4.0 dendrimer-like polyamidoamine (PAMAM) immobilized silica gel (PAMAMSG) was prepared with a divergent method by repeating two processes: (1) Michael addition of methyl acrylate (MA) to surface amino groups; and (2) amidation of the resulting esters with ethylenediamine (EDA) from gamma-aiminopro... |
15875863 | Hydration of black foam films made of amphiphilic cyclodextrins. | We investigate the interfacial behavior of a new type of amphiphilic cyclic oligosaccharide obtained by grafting a phospholipid onto a methylated cyclodextrin. These compounds are able to form stable black foam films, the structure of which can be determined using X-ray reflectivity. These films consist of a highly hyd... |
15875856 | Atomic force microscopy study of the adsorption and electrostatic self-organization of poly(amidoamine) dendrimers on mica. | The adsorption behavior of poly(amidoamine) dendrimers to mica surfaces was investigated as a function of ionic strength and pH. The conformation and lateral distribution of the adsorbed dendrimers of generations G8 and G10 were obtained ex situ by tapping mode atomic force microscopy (AFM). The deposition kinetics of ... |
15875855 | AFM observation of cation complexation of dibenzocrown ethers adsorbed on highly oriented pyrolytic graphite. | The cation complexation behavior of dibenzocrown ethers adsorbed on highly oriented pyrolytic graphite substrates was investigated by means of atomic force microscopy using probe tips modified chemically with ammonium ion by silane coupling. The specific adhesion force based on the intermolecular force between dibenzoc... |
15875408 | Liposome induced self-assembly of gold nanoparticles into hollow spheres. | Gold nanoparticles were prepared by the reduction of [(C7H15)4N]+ [AuCl4]- with 3,4-ethylenedioxythiophene (EDOT) as reductant in toluene solution. The employed stabilizers include 3,3'-thiodipropionic acid (TDPA), 1-dodecanethiol (DDT), (+/-)-10-camphorsulfonic acid (CSA), and 11-mercaptoundecanoic acid (MUA). The rea... |
15875403 | Methods for generating protein molecular ions in ToF-SIMS. | One of the greatest challenges in mass spectrometry lies in the generation and detection of molecular ions that can be used to directly identify the protein from the molecular weight of the molecular ion. Typically, proteins are large (MW > 1000), nonvolatile, and/or thermally labile, but the vaporization process produ... |
15875372 | Effect of the counterion on the rate of electron transfer in dendrimer films. | The electrochemical behavior of a film composed of a redox-active dendrimer was studied as a function of the type of counterion available during its reduction and reoxidation. The rate of permeation/migration of counterions into the film appeared to be the bottleneck to electron transfer through the film. Because the d... |
15875370 | Site-directed molecular assembly on templates structured with electron-beam lithography. | We describe a simple method for patterning biomolecular films on surfaces with high resolution. A conventional polymeric resist is structured by electron-beam lithography. The exposed and developed patterns are then used for the directed self-assembly (SA) of a first molecule from solution. Removal of the remaining res... |
15059283 | Alterations at the peptidyl transferase centre of the ribosome induced by the synergistic action of the streptogramins dalfopristin and quinupristin. | <h4>Background</h4>The bacterial ribosome is a primary target of several classes of antibiotics. Investigation of the structure of the ribosomal subunits in complex with different antibiotics can reveal the mode of inhibition of ribosomal protein synthesis. Analysis of the interactions between antibiotics and the ribos... |
15766141 | [Comparative study of room-temperature phosphorescence of 1-bromonaphthalene induced by synergetic effect of nonionic surfactants and beta-cyclodextrin]. | Room-temperature phosphorescence of 1-BrN induced by a combination of OPE-10 and Triton X-100 with beta-CD was comparatively studied. In terms of molecular size and dimensions of beta-CD, the octyl group and phenyl group of OPE-10 and Triton X-100 were incorporated into the cavity of beta-CD and the complexes with the ... |
15468234 | Chiral polymers containing a carbohydrate moiety: synthesis. | This paper reports the preparation of poly(acetal-ethers) by two alternative synthetic pathways (alkaline and acid-catalyzed conditions). Polycondensations of methyl 2,3:4,6-di-O-salicylidene-alpha-D-mannopyranoside (3) (mixtures of endo-H and exo-H dioxolan-2-yl-diastereomers) with 1,4-dibromobutane (4) (method I a an... |
15468229 | Peptide/protein hybrid materials: enhanced control of structure and improved performance through conjugation of biological and synthetic polymers. | The conjugation of peptides/proteins and synthetic polymers is a useful strategy to overcome some of the limitations related to the use of the individual components. This review will highlight two aspects: enhanced structural control at the nanometer level and improved performance, in particular with respect to biomedi... |
15267690 | Tunable effective interactions between dendritic macromolecules. | We employ extensive Monte Carlo and molecular-dynamics simulations to investigate the effective interactions between the centers of mass of dendritic macromolecules of variable flexibility and generation number. Two different models for the connectivity and steric interactions between the monomers are employed, the fir... |
15267501 | Radiative decay of nonstationary system. | When a finite quantum system, say a fluorescent molecule is attached to a bulk surface and excited by a short laser pulse, the decay dynamics of the system is modulated by the surface and the signal is enhanced due to the bulk surface. We have considered the decay dynamics of a model of displaced distorted molecule who... |
15256065 | Soluble, insoluble and geometric signals sculpt the architecture of mineralized tissues. | Bone morphogenetic and osteogenic proteins (BMPs/OPs), members of the transforming growth factor-beta (TGF-beta) superfamily, are soluble mediators of tissue morphogenesis and induce de novo endochondral bone formation in heterotopic extraskeletal sites as a recapitulation of embryonic development. In the primate Papio... |
15022200 | Chiral discrimination studies of (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid by high-performance liquid chromatography and NMR spectroscopy. | Chiral discrimination studies using (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA) as a chiral selector were performed by high-performance liquid chromatography (HPLC) and NMR spectroscopy. The enantiomers of alanine (Ala) or alanine methyl ester (Ala-ME) were well separated on the chiral stationary phases... |
15203892 | Pulmonary absorption enhancement of salmon calcitonin. | The aim of this work was to develop formulations of calcitonin for pulmonary delivery to enhance the absorption and study the comparative pharmacodynamic behavior of developed formulations in rats. Formulations with different pH, absorption promoters of different classes and combination thereof at three concentration l... |
15203885 | Cross-linking in collagen by nonenzymatic glycation increases the matrix stiffness in rabbit achilles tendon. | Nonenzymatic glycation of connective tissue matrix proteins is a major contributor to the pathology of diabetes and aging. Previously the author and colleagues have shown that nonenzymatic glycation significantly enhances the matrix stability in the Achilles tendon (Reddy et al., 2002, Arch. Biochem. Biophys., 399, 174... |
15202568 | PEGylation significantly affects cellular uptake and intracellular trafficking of non-viral gene delivery particles. | In vitro studies of non-viral gene delivery vectors are typically not performed at physiological conditions, and thus may not provide meaningful results for in vivo investigations. We determine if polycation-plasmid DNA complexes (polyplexes) exploited for in vitro studies behave similarly to variants more applicable t... |
15198207 | Transgenic rice as a vehicle for the production of the industrial enzyme transglutaminase. | Transglutaminases have a range of catalytic activities, most of which concern the post-translational modification of proteins. The most important of these activities is the cross-linking of proteins into large supramolecular networks. The widespread use of transglutaminases has increased the demand for an inexpensive, ... |
15179138 | Bacterial DNA and RNA induce rat cardiac myocyte contraction depression in vitro. | Sepsis and septic shock, the systemic immunologic and pathophysiologic response to overwhelming infection, are associated with perturbation of a variety of metabolic cell pathways and with multiple organ failure (MOF) including cardiac depression. This depression has been attributed to the effect of several circulating... |
15179127 | Transfusion of the injured patient: proceed with caution. | Transfusion of the injured patient with packed red blood cells (PRBCs) is a dynamic process requiring vigilance during the acute resuscitative and recovery phases postinjury. Although adverse events have been reported in 2% to 10% of injured patients, the advent of new detection techniques for viral pathogens has marke... |
15165710 | Metabolism and absorption enhancement of methionine enkephalin in human nasal epithelium. | The objective of this study was to investigate absorption enhancing approaches for systemic delivery of methionine enkephalin via the nose. Absorption promotion of methionine enkephalin in the presence of protease inhibitors (bestatin, puromycin) and absorption enhancers (glycocholate, dimethyl-beta-cyclodextrin) were ... |
15139519 | Contribution of cholesterol and phospholipids to inhibitory effect of dimethyl-beta-cyclodextrin on efflux function of P-glycoprotein and multidrug resistance-associated protein 2 in vinblastine-resistant Caco-2 cell monolayers. | <h4>Purpose</h4>The purpose of this study is to reveal the contribution of membrane components to the inhibitory effect of 2,6-di-O-methyl-beta-cyclodextrin (DM-beta-CyD) on P-glycoprotein (P-gp) and multidrug resistance-associated protein 2 (MRP2) function in vinblastine-resistant Caco-2 (Caco-2R) cell monolayers.<h4>... |
15139516 | Molecular reactivity of busulfan through its experimental electrostatic properties in the solid state. | <h4>Purpose</h4>In the route of developing novel liquid phase formulations based on the encapsulation of busulfan into liposomes in nontoxic solvents, drug crystallization inevitably occurs. In order to better understand the reactivity of busulfan, the characterization of its molecular properties was therefore consider... |
15126401 | Drosophila male-specific lethal 2 protein controls sex-specific expression of the roX genes. | The MSL complex of Drosophila upregulates transcription of the male X chromosome, equalizing male and female X-linked gene expression. Five male-specific lethal proteins and at least one of the two noncoding roX RNAs are essential for this process. The roX RNAs are required for the localization of MSL complexes to the ... |
15116966 | Solid-phase microextraction of phenol compounds using a fused-silica fiber coated with beta-cyclodextrin-bonded silica particles. | A novel solid-phase microextraction (SPME) fiber was prepared by coating an HPLC beta-cyclodextrin bonded silica stationary phase (CDS) on the surface of a fused-silica fiber. The fiber was evaluated for the determination of five phenol compounds (phenol, 2,4-dimethylphenol, 4-nitrophenol, 3-chlorophenol, 4-methylpheno... |
15116963 | Potentiometric determination of dopamine in pharmaceutical preparations by crown ether-PVC membrane sensors. | Two simple, rapid and sensitive sensors for the assay of dopamine hydrochloride have been developed. The methods are based upon the formation of the membrane sensors 12-crown-4-phosphotungestic acid (crown ether-PTA)-dopamine and 12-crown-4-tetraphenylborate (crown ether-TPB)-dopamine as neutral carriers. The sensors w... |
15116935 | Use of dynamically coated capillaries for the determination of heroin, basic impurities and adulterants with capillary electrophoresis. | Rapid, precise, accurate, and reproducible methodology using capillary electrophoresis (CE) with dynamically coated capillaries for the analysis of heroin and its basic impurities and adulterants is presented. Highly selective determination of the above solutes is obtained by analyzing the same sample preparation by tw... |
15116933 | Separation of quaternary ammonium diastereomeric oligomers by capillary electrophoresis. | The separation of novel diastereomeric trimers (3M) and pentamers (5M), derived from quaternary ammonium salts, was studied in conventional, uncoated and coated capillaries using capillary zone electrophoresis (CZE) with a variety of buffers and additives. Resolution of 5M diastereomers was best achieved using gamma-cy... |
15116926 | Enantioseparation of four cis and trans diastereomers of 2',3'-didehydro-2',3'-dideoxythymidine analogs, by high-performance liquid chromatography and capillary electrophoresis. | Compounds 1-4 are the four stereoisomers of a synthetic new potential antiviral agent (d4T analog) containing two chiral centers and a base (uracil). Both high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) techniques were used to separate and quantify enantiomers with high resolution. The ... |
15116909 | Capillary microextraction on sol-gel dendrimer coatings. | Sol-gel capillary microextraction (CME) is a new direction in the solventless sample preparation for the preconcentration of trace analytes, and presents significant interest in environmental, pharmaceutical, petrochemical, biomedical, agricultural, food, flavor, and a host of other important areas. It utilizes advance... |
15108713 | Ultrasensitive detection of 1,4-Bis(4-vinylpyridyl)phenylene in a small volume of low refractive index liquid by surface-enhanced Raman scattering-active light waveguide. | This paper reports a novel surface-enhanced Raman scattering (SERS)-active light waveguide method for ultrasensitive detection of a sample dissolved in a small volume of low refractive index liquid. The SERS-active light waveguide demonstrated in this study was constructed via the light-guiding silica capillary. The su... |
15101750 | Demetalation of the regioselective oxygenation product of an N-confused porphyrin complex. | [structure: see text] The demetalation of M(III)(HCTPPO)Br (M = Mn or Fe) afforded two hydroxylated N-confused porphyrinoids. CTPPOH retains the tautomer form of the N-confused porphyrin with a hydroxyl group substituted in the inner-core carbon. The further attack of OH(-) to the meso carbon afforded a dihydroxylated ... |
15101745 | A supramolecular approach to protein labeling. A novel fluorescent bioassay for concanavalin a activity. | [reaction: see text] A new method for the bioassay of concanavalin A is based on the interaction of saccharide-substituted oligopyrrolic macrocycles with lectins. A general sensing mechanism involves the interaction of aggregated and primarily nonfluorescent labels with the target protein, label deaggregation, site-spe... |
15101742 | Novel chiral dendritic diphosphine ligands for Rh(I)-catalyzed asymmetric hydrogenation: remarkable structural effects on catalytic properties. | [reaction: see text] A series of dendritic ligands with a chiral diphosphine located at the focal point have been synthesized through coupling of pyrphos 2 with Fréchet-type polyether dendron 3. The relationship between the primary structure of the dendrimer and its catalytic properties was established in the Rh-cataly... |
15095454 | Chiral capillary electrophoretic resolution of baclofen, gabaergic ligand, using highly sulfated cyclodextrins. | Using cyclodextrin-capillary zone electrophoresis (CD-CZE), baseline separation of baclofen, a potent GABA(B) agonist; was achieved. A method for the enantioresolution of this gamma-aminobutyric acid (GABA) and determination of enantiomeric purity was developed using CDs (highly sulfated-CD or highly S-CD) as chiral se... |
15095453 | Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis. | For the determination of iodide in urine, where 80-90% of consumed iodine is excreted, a fast, simple, and sensitive method of capillary zone electrophoresis was elaborated and tested also for additional complex matrices such as human serum, cooking salt, and seawater. Several approaches were examined for the separatio... |
15093832 | High-sensitivity mass spectrometry for imaging subunit interactions: hydrogen/deuterium exchange. | In recent years, advances in mass spectrometry have provided unprecedented knowledge of protein expression within cells. It has become apparent that many proteins function as macromolecular complexes. Structural genomics programs are determining the fold of these proteins at an increasing rate and electron microscopic ... |
15093828 | A structural view of the COPII vesicle coat. | The COPII vesicle coat coordinates the budding of transport vesicles from the endoplasmic reticulum in the initial step of the secretory pathway. The coat orchestrates a sequence of events including self-assembly on the membrane, cargo and SNARE molecule selection, and deformation of the membrane into a bud to drive ve... |
15081220 | Controlling the mechanism of trypsin inhibition by the numbers of alpha-cyclodextrins and carboxyl groups in carboxyethylester-polyrotaxanes. | Carboxyethylester-polyrotaxanes (CEE-polyrotaxanes) with the various number of CEE-modified alpha-cyclodextrins (CEE-alpha-CDs) were synthesized, and the effects of the number of CEE-alpha-CDs on calcium binding and trypsin inhibition were investigated. Calcium binding affinity was dependent on the density of the CEE g... |
15072304 | Sulfamethoxazole-imprinted polymer for selective determination of sulfamethoxazole in tablets. | A sulfamethoxazole (SMO)-imprinted polymer (MIP) was prepared in acetonitrile using the mixture of acrylamide and 4-vinylpyridine as functional monomers. The molecular recognition properties of the polymer was evaluated in both acetonitrile and aqueous acetonitrile mobile phases. SMO contents in two kinds of tablets we... |
15072302 | Separation and migration behavior of positional and structural naphthalenesulfonate isomers by cyclodextrin-mediated capillary electrophoresis. | The effects of the type of buffer system, buffer pH, the polarity of electrode, and both the type and the concentration of cyclodextrins (CDs) on the separation and migration behavior of seven positional and structural naphthalenesulfonate isomers in CD-mediated capillary electrophoresis were systematically investigate... |
15072298 | Molecular interconversion behaviour in comprehensive two-dimensional gas chromatography. | Comprehensive two-dimensional gas chromatography (GC x GC) is shown to provide information on dynamic molecular behaviour (interconversion), with the interconversion process occurring on both columns in the coupled-column experiment. The experiment requires suitable adjustment of both experimental conditions and relati... |
15072296 | Characterization of drug interactions with soluble beta-cyclodextrin by high-performance affinity chromatography. | This study examined the use of an immobilized human serum albumin (HSA) column to study solution-phase reactions between drugs and beta-cyclodextrin (beta-CD). Chromatographic equations were developed to characterize the binding of chemicals to a soluble ligand (beta-CD) in the presence of an independent immobilized li... |
15072293 | Effect of the eluent on enantiomer separation of controlled drugs by liquid chromatography-ultraviolet absorbance detection-electrospray ionisation tandem mass spectrometry using vancomycin and native beta-cyclodextrin chiral stationary phases. | Enantioseparation of nine amphetamine derivatives, methorphan and propoxyphene was studied by comparing two different chiral stationary phases, macrocyclic antibiotic vancomycin and native beta-cyclodextrin (beta-CD). Effects of 46 eluent compositions on enantioseparation in reversed-phase (RP) and polar organic phase ... |
15070324 | Thioacylation reactions for the surface functionalization of phosphorus-containing dendrimers. | The functionalization of phosphorus-containing dendrimers was easily achieved through thioacylation reactions involving new dendrimers capped with dithioester end groups and various functionalized amines. These reactions were successfully applied to the first generation (12 end groups) and the third generation of the d... |
15070303 | Facile purification of rare cucurbiturils by affinity chromatography. | A practical method for the separation and purification of cucurbituril (CB) hexamers was developed on the basis of affinity chromatography using aminopentylaminomethylated polystyrene beads. This recyclable resin, which can be used repeatedly, facilitates the general preparation of cucurbituril derivatives and compensa... |
15070297 | Mannich reaction as a new route to pyridine-based polyaminocarboxylic ligands. | The Mannich reaction was successfully employed to obtain two pyridine-based ligands 1 and 2. The latter represents the first example of a Mannich reaction leading to a 12-membered pyridine-containing macrocycle. [reaction: see text] |
15070296 | Synthesis, X-ray structure, and self-assembly of functionalized bis(2,2':6',2' '-terpyridinyl)arenes. | meta-Bis(terpyridinyl)phenol has been synthesized from O-benzyl-3,5-di(formyl)phenol in three steps. Its alkylation afforded a series of bisterpyridinylarenes, which can be self-assembled to afford the corresponding hexametallomacrocycles possessing Fe(II), Zn(II), or Ru(II) connectivity. [structure: see text] |
15070294 | Exceptional chiral recognition of racemic carboxylic acids by calix[4]arenes bearing optically pure alpha,beta-amino alcohol groups. | Calixarenes bearing optically pure alpha,beta-amino alcohol groups at their lower rim exhibit exceptional and efficient chiral recognition ability in discrimination of racemic mandelic acid, 2,3-dibenzoyltartaric acid and 2-hydroxy-3-methylbutyric acid. [structure: see text] |
15066667 | The effect of pH dependent molecular conformation and dimerization phenomena of piroxicam on the drug:cyclodextrin complex stoichiometry and its chromatographic behaviour. A new specific HPLC method for piroxicam:cyclodextrin formulations. | The complexation of piroxicam with various cyclodextrins was studied in the whole pH range, in an attempt to clarify the reported differences in stoichiometry of the resulting inclusion complexes. In this work, it was found that the pH dependent self-association phenomena, detected in both alkaline and acidic media, ar... |
15066663 | Cell transfection with polycationic cyclodextrin vectors. | Polycationic cyclodextrins (CDs) were complexed with plasmid DNA and their effectiveness as vectors was tested on COS-7 cells. These CDs were modified with pyridylamino, alkylimidazole, methoxyethylamino or primary amine groups at 6-positions of the glucose units. Uncharged CDs, beta-CD, hydroxypropyl-beta-CD, and dime... |
15065806 | Assay of phenolic compounds in red wine by on-line combination of capillary isotachophoresis with capillary zone electrophoresis. | The on-line combination of capillary zone electrophoresis (CZE) with capillary isotachophoresis (ITP) increases significantly the separation capability and sensitivity of capillary electrophoresis. This technique was used for separation and quantification of fourteen selected natural constituents in red wine belonging ... |
15065800 | Enantioseparation of benzoins and enantiomer migration reversal of hydrobenzoin in capillary zone electrophoresis with dual cyclodextrin systems and borate complexation. | Enantioseparations of racemic hydrobenzoin and structurally related compounds, including benzoin and benzoin methyl ether, in capillary zone electrophoresis (CZE) with dual cyclodextrin (CD) systems consisting of S-beta-CD (mixed isomers) and a neutral CD, including beta-CD and hydroxypropyl-beta-CD (HP-beta-CD), as ch... |
15065799 | Resolution of indobufen enantiomers by capillary zone electrophoresis. Pharmacokinetic studies of human serum. | A direct and stereospecific method was worked out to quantify indobufen enantiomers in human serum using capillary zone electrophoresis (CZE). The indobufen enantiomers and (+)-S-ketoprofen (internal standard, IS) were separated in a fused silica capillary, filled with heptakis 2,3,6-tri-O-methyl-beta-cyclodextrin as a... |
15065798 | Comparative studies on the enantioseparation of hydrobenzoin and structurally related compounds by capillary zone electrophoresis with sulfated beta-cyclodextrin as the chiral selector in the presence and absence of borate complexation. | Comparative studies on the enantioseparations of racemic hydrobenzoin, together with benzoin and benzoin methyl ether, in capillary zone electrophoresis using sulfated beta-cyclodextrin (S-beta-CD) as a chiral selector in the presence and absence of borate complexation were investigated. The influences of S-beta-CD con... |
15065792 | Determination of binding constants of hydrophobically end-capped poly(ethylene glycol)s with beta-cyclodextrin by affinity capillary electrophoresis. | The formation of inclusion complexes between methoxypoly(ethylene glycol)s (MPEG)s bearing one hydrophobic group (phenyladamantyl) per chain and beta-cyclodextrin (beta-CD) was studied by capillary electrophoresis (CE). The effect of highly sulphated beta-CD (HS-beta-CD) on the migration behaviour of the phenyladamanty... |
15065775 | Determination of bisphenol A in environmental water at ultra-low level by high-performance liquid chromatography with an effective on-line pretreatment device. | We have developed a simple HPLC method for the microanalysis of bisphenol A (BPA), which is often contained in environmental water and is known as an endocrine disrupter. HPLC coupled with electrochemical detection requires a simpler procedure of pretreatment compared to GC-MS. In this study, we analyzed BPA using mole... |
15063471 | Comparative photodegradation studies on 3-hydroxyflavone: influence of different media, pH and light sources. | 3-Hydroxyflavone (3-OH-F) photochemistry in solution has been rationalized in terms of an excited state intramolecular proton transfer (ESIPT), which involves the free 3-hydroxy group interacting with the ortho-carbonyl. This photo-rearrangement occurs rapidly and is strongly influenced by the physico-chemical properti... |
15063470 | Improvement in solubility and dissolution rate of flavonoids by complexation with beta-cyclodextrin. | The inclusion into the beta-cyclodextrin is used to improve pharmacokinetic characteristics of hesperetin and naringenin. Solubility of hesperetin and naringenin with increasing concentrations of beta-cyclodextrin grows as long as the temperature increased. Stability constants were determined by the solubility method b... |
15063469 | Effects of alpha- and beta-cyclodextrin complexation on the physico-chemical properties and antioxidant activity of some 3-hydroxyflavones. | Inclusion complexes of some flavonols (3-hydroxyflavone, morin and quercetin) have been obtained with alpha- and beta-cyclodextrins, by the co-evaporation method. Different analytical techniques (DSC, XRPD, FT-IR, 1H-NMR, UV-Vis) have been employed for a throughout investigation of the structural characteristics of suc... |
15063461 | Photostability studies on nicardipine-cyclodextrin complexes by capillary electrophoresis. | Nicardipine (NC)-cyclodextrin solid systems were prepared in equimolar ratios and their photostability in aqueous solution under exposure to UV(A)-UV(B) radiations was evaluated. The photodegradation process was monitored by a capillary electrophoresis (CE) method able to provide the enantioresolution of the rac-nicard... |
15063460 | HPLC enantiomer separation of a chiral 1,4-dihydropyridine monocarboxylic acid. | An enantioselective anion exchanger based on tert-butylcarbamoylquinine as chiral selector and thiol-modified silica as chromatographic support was applied for the enantiomer separation of the shortacting calcium antagonist clevidipine after its hydrolysis to methyl 4-(2',3'-dichlorophenyl)-2,6-dimethyl-1,4-dihydropyri... |
15063210 | X-ray crystallography and solution NMR spectroscopy characterization of heptakis(2,3-di-O-acetyl-6-bromo-6-deoxy)cyclomaltoheptaose. | Heptakis(2,3-di-O-acetyl-6-bromo-6-deoxy)cyclomaltoheptaose has been characterized in aqueous solution by 1D and 2D NMR spectroscopy and in the solid state by X-ray crystallography. In methanol solution, the acetyl groups were found to interact with both inward and outward-pointing protons. This and the strong deshield... |
15056847 | Regulation of ribozyme activity with short oligonucleotides. | Both hammerhead and hairpin ribozymes, which are well known as small catalytic RNAs, can catalyze either cleavage or ligation of RNA with sequence specificity. Although they have been applied to gene therapy and gene discovery, by cutting the mRNAs of target genes, these ribozymes normally do not have the ability to re... |
15054759 | Realization of unusual ligand binding motifs in metalated container molecules: synthesis, structures, and magnetic properties of the complexes [(LMe)Ni2(mu-L')]n+ with L'=NO3-, NO2-, N3-, N2H4, pyridazine, phthalazine, pyrazolate, and benzoate. | A series of dinickel(II) complexes with the 24-membered macrocyclic hexaazadithiophenol ligand H(2)L(Me) was prepared and examined. The doubly deprotonated form (L(Me))(2-) forms complexes of the type [(L(Me))Ni2II(mu-L')](n+) with a bioctahedral N(3)Ni(II)(mu-SR)(2)(mu-L')Ni(II)N(3) core and an overall calixarene-like... |
15054755 | First systematic investigation of C-H...Cl hydrogen bonding using inorganic supramolecular synthons: lamellar, stitched stair-case, linked-ladder, and helical structures. | Using a group of six neutral M(II)Cl(2)-containing coordination compounds as building blocks, the first systematic investigation of C-H...Cl hydrogen-bonding interactions was performed. Single-crystal X-ray structural analyses of four new compounds (pseudo-tetrahedral Co(II) and Zn(II); distorted trigonal bipyramidal Z... |
15053672 | Analysis of organoselenium compounds in human urine using active carbon and chemically modified silica sol-gel surface-assisted laser desorption/ionization high-resolution time-of-flight mass spectrometry. | Laser desorption/ionization time-of-flight mass spectra of three thermally labile low molecular weight organoselenium compounds (selenomethionine, selenoethionine, trimethylselenonium iodide) in human urine matrix have been obtained by using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Fou... |
15037243 | FtsH exists as an exceptionally large complex containing HflKC in the plasma membrane of Escherichia coli. | FtsH is an ATP-dependent and membrane-associated protease, which exerts processive proteolysis against membrane-embedded and soluble substrate proteins. Although previous studies suggested that it functions as a homo-oligomer and it also interacts with HflK-HflC membrane protein complex (HflKC), it is still important t... |
15028259 | Determination of 3(1)-stereochemistry in synthetic bacteriochlorophyll-d homologues and self-aggregation of their zinc complexes. | Zinc complex of methyl 3(1)-octadecyl-bacteriopheophorbide-d was prepared from modification of naturally occurring chlorophyll-a. The 3(1)-epimerically pure samples were obtained by HPLC separation and their stereochemistry including the absolute configuration at the secondary alcoholic 3(1)-position was determined by ... |
15026273 | Serum cytochrome c level as a prognostic indicator in patients with systemic inflammatory response syndrome. | <h4>Background</h4>Apoptosis may play an important role in the development of systemic inflammatory response syndrome (SIRS) and progression to multiple organ dysfunction syndrome (MODS). To quantify the extent of apoptosis in these morbidities, we developed a sandwich ELISA system to measure serum cytochrome c (cyt-c)... |
15018789 | Analysis of glycerophosphoinositol by liquid chromatography-electrospray ionisation tandem mass spectrometry using a beta-cyclodextrin-bonded column. | Glycerophosphoinositol (GroPIns) has been demonstrated to have important roles in many intracellular regulatory processes. GroPIns has been analysed for many years by anion-exchange HPLC after radiolabelling of cells in culture, but no method has been developed, to our knowledge, for the direct detection and quantitati... |
15019972 | Inclusion complexes of carotenoids with cyclodextrins: 1H NMR, EPR, and optical studies. | Direct evidence of carotenoid/cyclodextrin inclusion complex formation was obtained for the water-soluble sodium salt of beta-caroten-8'-oic acid (IV) by using 1H NMR and UV-Vis absorption spectroscopy. It was shown that this carotenoid forms a stable 1:1 inclusion complex with beta-cyclodextrin (stability constant K11... |
15003605 | Monitoring protein modification with allosteric ribozymes. | An allosteric ribozyme is an RNA-based enzyme (ribozyme) whose catalytic activity is modulated by molecular recognition of a protein. The direct coupling of a detectable catalytic event to molecular recognition by an allosteric ribozyme enables simple assays for quantitative protein detection. Most significantly, the m... |
15332598 | Controlling the supramolecular organisation of adsorbed collagen layers. | The supramolecular organisation of collagen adsorbed on polymer substrates was investigated as a function of properties of the substrates (chemical nature, roughness) and of characteristics of the collagen solution (concentration, state of aggregation) as well as details of the preparation procedure (adsorption time, d... |
15190932 | Reactions of superoxide radicals with curcumin: probable mechanisms by optical spectroscopy and EPR. | Reactions of superoxide-crown ether complex with curcumin have been studied in acetonitrile. Optical absorption spectra showed that curcumin on reaction with superoxide forms a blue color intermediate absorbing at 560 nm, which subsequently decayed in a few hours with the development of the absorption band correspondin... |
15185376 | Synthesis of metal-carbonyl-dendrimer-antibody immunoconjugates: towards a new format for carbonyl metallo immunoassay. | We report the preparation of metal-carbonyl-dendrimer-antibody conjugates. These metal-carbonyl-multilabeled antibodies are designed to be used in a new solid-phase-format carbonyl metallo immunoassay (CMIA). A fourth-generation polyamidoamine dendrimer was labeled with 10-25 (eta5-cyclopentadienyl)iron dicarbonyl (eta... |
15164605 | [A novel molecular marker for thrombus formation and life prognosis--clinical usefulness of measurement of soluble fibrin monomer-fibrinogen complex (SF)]. | For a long time fibrinopeptide A(FPA), fibrinopeptide B(FPB), D-dimer, FM test, serum FDP, and thrombin anti-thrombin complex(TAT) are being used as molecular markers to for sure diagnose hypercoagulable state and thrombus formation. Indeed these molecular markers are very useful for diagnosing thrombus formation, diss... |
15157331 | [In vivo transfection and expression of human coagulant factor VIII cDNA in mice]. | The aim is to observe the expression of human factor VIII gene in mice tranduced in vivo and ex vivo. The vector pLNC-FVIII BD was generated by cloning a B-domain-deleted (760aa-1639aa) FVIII cDNA (FVIIIBD cDNA) into retroviral vector pLNCX. 2 x 10(6) of mouse bone marrow stroma cells transduced by LNC-FVIII BD were in... |
15139222 | Structure and dynamics of self-assembled poly(ethylene glycol) based coiled-coil nano-objects. | Herein we describe the structure and dynamics of self-assembled nano-objects generated from poly(ethylene glycol) based (PEG-ylated) coiled-coil hybrid block copolymers. Electron paramagnetic resonance (EPR) experiments on spin-labeled samples provided a strong indication for a parallel alignment of the peptide helices... |
15139220 | Ground and excited-state electronic interactions in poly(propylene amine) dendrimers functionalized with naphthyl units: effect of protonation and metal complexation. | We report the absorption spectra and the photophysical properties (fluorescence spectrum, quantum yield, and lifetime) of four dendrimers of the poly(propylene amine) family (POPAMs) functionalized at the periphery with naphthylsulfonamide (hereafter called naphthyl) units. Each dendrimer Gn, where n = 1 to 4 is the ge... |
15119405 | Optical amplification in a first-generation dendritic organic semiconductor. | We report a study of a new class of organic semiconductor as an optical gain medium. We demonstrate amplification of violet light by use of stimulated emission in a solution of a first-generation bis-fluorene-cored semiconducting dendrimer. Amplification is also observed in the solid state by means of amplified spontan... |
15117968 | Sequential exocytosis of insulin granules is associated with redistribution of SNAP25. | We have investigated sequential exocytosis in beta cells of intact pancreatic islets with the use of two-photon excitation imaging of a polar fluorescent tracer, sulforhodamine B, and a fusion protein comprising enhanced cyan fluorescent protein (ECFP) and the SNARE protein SNAP25 (synaptosome-associated protein of 25 ... |
15117072 | Direct high-performance liquid chromatographic method for enantioselective and diastereoselective determination of selected pyrethroic acids. | This study reports on the direct HPLC stereoisomer separation of selected pyrethroic acids employing commercial cinchona alkaloid derived chiral stationary phases (CSPs). cis/trans-Chrysanthemic acid (cis/trans-CA), cis/trans-chrysanthemum dicarboxylic acid (cis/trans-CDCA), cis/trans-permethrinic acid (cis/trans-PA), ... |
15115815 | A synthetic neural cell adhesion molecule mimetic peptide promotes synaptogenesis, enhances presynaptic function, and facilitates memory consolidation. | The neural cell adhesion molecule (NCAM) plays a critical role in development and plasticity of the nervous system and is involved in the mechanisms of learning and memory. Here, we show that intracerebroventricular administration of the FG loop (FGL), a synthetic 15 amino acid peptide corresponding to the binding site... |
15115813 | Peripheral treatment with enoxaparin, a low molecular weight heparin, reduces plaques and beta-amyloid accumulation in a mouse model of Alzheimer's disease. | We investigated the effect of long-term, peripheral treatment with enoxaparin, a low molecular weight heparin, in transgenic mice overexpressing human amyloid precursor protein(751). Enoxaparin (6 IU per mouse intraperitoneally, three times a week for 6 months) significantly lowered the number and the area occupied by ... |
15110619 | Heart transplantation in patients with amyloidosis: single-center experience. | <h4>Introduction</h4>Amyloidosis is a systemic disease. Heart transplantation in this subset of patients is contraindicated by the majority of authors. In our center, patients with heart failure due to amyloidosis have been evaluated for cardiac transplantation since 1991. The aim of this study was to analyze the outco... |
15110586 | Combined liver-kidney transplantation: experience at Bologna's transplant center. | <h4>Background</h4>The results obtained for combined liver-kidney transplantation (CLKT) are reported.<h4>Methods</h4>From 1997 to 2003 six procedures were performed in patients with polycystic disease (n = 2), chronic glomerulonephritis associated with HCV cirrhosis (n = 2), chronic interstitial nephritis associated w... |
15111110 | The G protein-coupled receptor rhodopsin in the native membrane. | The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes f... |
15104430 | Cyclic tetra- and hexaynes containing 1,4-donor-substituted butadiyne units: synthesis and supramolecular organization. | Cyclic bis(1,3-butadiynes) with sulfur centers placed in the alpha-position to the 1,3-butadiyne units (2(n)) were synthesized by Glaser coupling of the corresponding open chain dithia-alpha,omega-diynes 1(n). In a second protocol we applied a four-component cyclization by reacting alpha,omega-dithiocyanatoalkanes 6(n)... |
15104429 | Sequences in dendrons and dendrimers. | Sequential incorporation of a variety of functional groups forms the basis for specificity in biomacromolecules. Introduction of such diversity and sequencing ability in artificial macromolecules is fundamentally interesting. In this paper, three different synthetic approaches have been used to build dendrons and dendr... |
15104484 | Characterization of the preferred stereochemistry for the neuropharmacologic actions of antillatoxin. | Antillatoxin is a potent ichthyotoxin and cytotoxin previously discovered from the marine cyanobacterium Lyngbya majuscula. Ensuing studies of its mechanism of action showed it to activate the mammalian voltage-gated sodium channel at a pharmacological site that is distinct from any previously described. The structure ... |
15102911 | Rho kinase regulates schwann cell myelination and formation of associated axonal domains. | The myelin sheath forms by the spiral wrapping of a glial membrane around an axon. The mechanisms involved are poorly understood but are likely to involve coordinated changes in the glial cell cytoskeleton. Because of its key role as a regulator of the cytoskeleton, we investigated the role of Rho kinase (ROCK), a majo... |
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