PMC_ID stringlengths 8 11 | Image_num stringclasses 399 values | Online_file_path stringlengths 20 50 ⌀ | Image_info_Cleaned stringlengths 2 20.6k | Caption_Clean stringlengths 1 10.6k ⌀ | label stringclasses 2 values |
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PMC11406843 | Figure_3 | oa_package/b4/72/PMC11406843.tar.gz | [' 3A and D, and S3; t = 2.', ' 3B; t = 2.', ' 3C; t = 0.', ' 3E and H; t = 2.', ' 3I, J and t = 2.', ' 3K, L and t = 4.', '\nComplement promotes microglial engulfment of excitatory synapse in mouse hippocampus after surgery.', '0001, ns, not significant; by two-tailed t-test\nBlockage of C3aR prevents microglial activation and loss of excitatory synapse in the mouse modelWe next investigated whether the C3-C3aR axis also promotes excitatory synaptic pruning in the mouse model.'] | Fig. 3 Complement promotes microglial engulfment of excitatory synapse in mouse hippocampus after surgery. ( ) Immunofluorescence detection of VGluT1 (green) & Homer1 (red) colocalized puncta in the hippocampal stratum radiatum from the control and surgery group. Scale bars: 10m. ( ) Quantification of VGluT1 positive puncta ( =4 mice per group). ( ) Quantification of Homer1 positive puncta ( =4 mice per group). ( ) Quantification of VGluT1 & Homer1 colocalized puncta ( =4 mice per group). ( - ) Immunofluorescence detection of VGluT1 (green) & C1q (red) colocalized puncta in the hippocampal stratum radiatum from the control and surgery group. ( ) Representative images, scale bar: 20m and 5m. ( ) Quantification of C1q and VGluT1 colocalized puncta ( =4 mice per group). ( - ) Immunofluorescence detection of VGluT1 (green) & C3 (red) colocalized puncta in the hippocampal stratum radiatum from the control and surgery group. ( ) Representative images, scale bar: 10m and 4m. ( ) Quantification of C3 and VGluT1 colocalized puncta ( =4 mice per group). ( - ) Triple immunofluorescence staining of IBA1 (green), CD68 (blue) and VGluT1 (red) in the hippocampal stratum radiatum from the control and surgery group. ( ) Representative images and three-dimensional (3D) reconstruction. Scale bars: 10m and 3m. ( ) Quantification of VGluT1 positive puncta within microglial lysosomes ( =2127 cells from 4 mice per group). ( - ) Effect of C3 treatment on the phagocytosis activity of microglia cells in vitro. ( ) Representative immunofluorescence images showing the fluorescent beads (green) inside the primary microglial cells after C3 or PBS treatment. Scale bars: 100m. ( ) Quantification of the fluorescent beads taken up by primary microglial cells from ( ) (left: 67 fields from 3 batches per group; right: 3536 cells from 3 batches per group). Data in bar graph are presented as meanSEM. * <0.05, ** <0.01, *** <0.001, **** <0.0001, ns, not significant; by two-tailed -test | yes |
PMC11455700 | Figure_3 | oa_package/c9/0a/PMC11455700.tar.gz | [' 3) of a primary intranodal tumour in the right submandibular gland, with no evidence of further nodal or metastatic disease.', '\nFDG-PET scan demonstrating moderately avid lesion in right submandibular region\nThe core biopsy histology revealed classic features of epithelioid hemangioendothelioma presenting as an intranodal tumour.'] | Fig. 3 FDG-PET scan demonstrating moderately avid lesion in right submandibular region | yes |
PMC5640862 | Figure_3 | oa_package/12/a6/PMC5640862.tar.gz | ['In this study, titration series of IVIg, pooled human AB serum, and pooled cord blood plasma revealed a 4-fold-reduced neutralization on Vero2a compared to parental Vero cells (A to D).'] | FIG 3 Reduction of neutralization of RSV in FCGR2a-transduced Vero cells. (A to C) RSV neutralization assays using parental and FCGR2a-transduced Vero cells (Vero2a) in the presence of serially diluted IVIg, human serum, or cord blood. (D) The PRNT50 is a measure for the reduction of neutralization titer in Vero2a cells calculated by dividing PRNT50[Vero] by PRNT50[Vero2a] (depicted by the arrows in panels A to C). Means and SD from 3 individual experiments are shown. | yes |
PMC11573106 | Figure_5 | oa_package/a3/2e/PMC11573106.tar.gz | [] | FIGURE 5. (A) Subcutaneous microvascular thrombosis with or without endothelial localization of calcium was seen in about a third of cases. In (B) there is a higher power image of the thrombosed capillary. There is no viable endothelium lining the microvessel imparting a thrombosed eosinophilic mummified quality to the vessel. | yes |
PMC3518474 | Figure_1 | oa_package/92/43/PMC3518474.tar.gz | ['By immunohistochemical staining we could verify the expression of NRP1 in all layers of the epidermis in human and mouse skin sections ( A, B, green fluorescence, nuclear stain in red with propidium iodide).', '0050944-Hafner1" ref-type="bibr">[18] with mice homozygous for a mutated NRP1 gene containing two loxP-sites flanking the exon 2 ( C) <xref rid="pone.', 'Genotyping was performed by polymerase chain reaction ( D).', 'As shown by quantitative real time RT-PCR, the detectable amounts of NRP1 mRNA were below 20%, as compared to keratinocytes homozygous for the floxed NRP1 gene but lacking Cre recombinase activity ( E).', 'Using crysections of newborn mice (day 2 postnatally) we could confirm efficient ablation of NRP1 protein expression in the epidermal layers of conditional knock out mice ( F, G).', 'g001Expression and epidermis-specific deletion of neuropilin 1 in mice.', 'Additionally, NRP1 expression in the epidermis was assessed by immunofluorescent staining and demonstrated absence of detectable NRP1 protein in the epidermis of NRP1epi / mice ( F, G, green fluorescence, the broken line reflects the epidermal basement membrane).'] | 10.1371/journal.pone.0050944.g001 | yes |
PMC9768501 | Figure_4 | oa_package/d0/22/PMC9768501.tar.gz | ['(A D) Jejunum structure under 100 magnification during deltamethrin treatment for 0 (A), the 1st (B), 7th (C), and 14th day (D).'] | Figure 4 Jejunum structure under 100 magnification during deltamethrin treatment for 0 , the 1st , 7th , and 14th day . Jejunum structure under 100 magnification at the 1st , 7th , and 14th day during deltamethrin treatment withdrawal. A, intestinal villi; B, intestinal glands (intestinal crypts); C, local mucosal lymphocyte infiltration; D, absence of intestinal villi epithelium. | yes |
PMC10405522 | Figure_4 | oa_package/9a/f8/PMC10405522.tar.gz | ['5 years postoperatively and TB-CTA indicated recurrent Div with bony Deh ().', '(A) Preoperative axial temporal bone computed tomography (TBCT) image of Subject 4 shows a huge diverticulum protruding through the mastoid air cells and cortical mastoid bone to the level of the subcutaneous soft tissue layer (arrow).', 'Subjects 2, 3, 6, and 7 showed combined SS-Div/SS-Deh, and reshaping/resurfacing was performed in these four subjects using a cortical bone chip, a piece of temporalis muscle fascia, and bone cement (s 4, 5).'] | Figure 4 Preoperative axial temporal bone computed tomography (TBCT) image of Subject 4 shows a huge diverticulum protruding through the mastoid air cells and cortical mastoid bone to the level of the subcutaneous soft tissue layer (arrow). Postoperative axial TBCT image shows successful reduction of the diverticulum through transmastoid resurfacing with bone wax (arrow) and fibrin glue. Follow-up TBCT axial image shows a huge diverticulum protruding through the mastoid air cells and cortical mastoid bone to the level of the subcutaneous soft tissue layer (arrow). Again, the diverticulum is successfully reduced by transmastoid reshaping with harvested autologous cortical bone chips and bone cement (arrow). The diverticulum was exposed to the mastoid cortex again. Thus, revision reshaping of the sigmoid sinus diverticulum was performed with harvested autologous cortical bone chips and bone cement to reconstruct a secure sinus wall over the diverticulum. | yes |
PMC8354305 | Figure_2 | oa_package/6d/66/PMC8354305.tar.gz | [', with permission from ElsevierThrombus in a small pulmonary artery (green arrow), with small thrombus seen in adjacent pulmonary venule (green arrowhead), with H E present on the left, and CD61 immunostain highlighting platelets within the thrombi on the right.'] | Fig. 2 Histologic changes of coronavirus disease 2019 pneumonia in case 2. (A) Evident proteinaceous and fibrin exudate; (B) Diffuse expansion of alveolar walls and septa owing to fibroblastic proliferations and type II pneumocyte hyperplasia, consistent with early diffuse alveolar damage pattern; (C) Plugs of proliferating fibroblasts or fibroblast balls in the interstitium (arrow); (D) Abundant macrophages infiltrating airspaces and type II pneumocyte hyperplasia. Reproduced/adapted from Tian et al., with permission from Elsevier | yes |
PMC7235864 | Figure_3 | oa_package/7a/36/PMC7235864.tar.gz | ['To get this location, we placed the image into a calibrated graph as seen in .', 'Measuring a dental image where scale bars are measured in pixels.'] | Figure 3 Measuring a dental image where scale bars are measured in pixels. | yes |
PMC8713588 | Figure_1 | oa_package/4c/ad/PMC8713588.tar.gz | ['A day after this, he was prescribed oral penicillin V for a sore throat and developed the penile and mouth ulcers as soon as he took the antibiotics ().', '\n\nMouth ulcers.'] | Figure 1 Mouth ulcers. | yes |
PMC3350088 | Figure_3 | oa_package/ce/4a/PMC3350088.tar.gz | ['An MRI also showed complete radiologic response to treatment (s 3(b) and 4(b)).', '002"/>Comparison of bilateral breast MRI studies performed before and after neoadjuvant chemotherapy.'] | Figure 3 Comparison of bilateral breast MRI studies performed before and after neoadjuvant chemotherapy. (a) Doppler flow MRI before neoadjuvant chemotherapy. Yellow arrow points to the 2.7cm irregular mass in the right breast. (b) Doppler flow MRI after neoadjuvant chemotherapy shows complete radiologic response. Yellow arrow points to area where the mass was located prior to treatment. | yes |
PMC4752651 | Figure_1 | oa_package/21/7a/PMC4752651.tar.gz | ['Under CONV-housing, TNFdeltaARE mice developed tissue pathology in the terminal ileum at the age of 18 weeks (figure 1A, B).', 'In the absence of microorganisms (GF conditions), TNFdeltaARE mice were free of ileal (figure 1A, B) and colonic disease (see online supplementary figure S1A).', 'In the absence of inflammation (age of 4 weeks), bacterial phylogenetic make-ups in TNFdeltaARE and WT mice overlapped (figure 1C).', 'Ileitis was significantly reduced after VM-treatment and complete relapse of ileitis was achieved 6 weeks after ending the antibiotic treatment (figure 1D).', 'Tnf-expression levels reflected the dynamic changes in ileal disease activity (figure 1E).', 'Numbers of colony-forming units grown under anaerobic conditions (figure 1F) and total cell counts (see online supplementary figure S1D) showed no change after VM treatment, suggesting growth of VM-resistant bacterial species.', 'However, bacterial diversity was drastically reduced in VM-treated mice (figure 1G).', 'coli) (figure 1H).'] | Figure1 Changes in the gut microbial ecosystem are associated with inflammation in TNF mice. (A) Ileitis scores in 18-week-old TNF (ARE) and WT littermates in CONV or GF housing. Ileitis-score groups are colour-coded according to inflammation severity: score=0 (blue); score <4 (grey); score >4 (red). Male mice are displayed as triangles, female mice as circles. (B) Representative H&E-stained sections of the distal ileum. (C) NMDS plot showing shifts of centroids (indicated by arrows) and variance of faecal bacterial profiles (circled areas) of CONV-TNF and WT mice at 4 weeks, 8 weeks and 12weeks of age. 16S ribosomal RNA gene amplicons of the V3/V4 region (407bp) in faeces were sequenced on a MiSeq platform. (D) Ileitis scores in TNF mice treated for 4weeks with vancomycin and metronidazole (VM), starting at 8weeks of age. Recurrence of ileitis after VM therapy was followed for 6weeks (n=56/group). (E) Ileal expression of as fold-increase to CONV-WT mice (normalised to ). (F) Ileal bacterial density of cultivable anaerobes (as log 10 of colony forming units (cfu) per gram ileal content). (G) Intestinal bacterial diversity (Shannon effective counts). (H) Changes in caecal bacterial composition (relative abundance of total sequences) at the phylum level. *p<0.05; **p<0.01; ***p<0.001 (Two-way-ANOVA followed by Holm-Sidak test). CONV, conventional; GF, germ-free; ARE, AU-rich elements; TNF, tumour necrosis factor; NMDS, non-parametrical multiple dimensional scaling. | yes |
PMC4056706 | Figure_5 | oa_package/fb/fe/PMC4056706.tar.gz | ['16 The lesion usually consists of sheets of clear\ncells with vesicular nuclei that occupy the reticular dermis and may extend into the\nsubcutis ().'] | FIGURE 3 Epithelioid fibrous histiocytoma: rounded epithelioid cells with abundanteosinophilic cytoplasm (H&E 100x) | yes |
PMC7367563 | Figure_2 | oa_package/72/e4/PMC7367563.tar.gz | ['8% (4/13) [].', '4% (2/13) each, Wyburn Mason (WB) syndrome [], and Bannayan Riley Ruvalcaba (BRR) syndrome in 7.'] | Figure 2 WB syndrome showing pulsatile, bluish tinged tortuous vessels on the left side of the forehead and frontal scalp, and ill-defined swelling of the left cheek | yes |
PMC5523561 | Figure_2 | oa_package/d9/37/PMC5523561.tar.gz | ['TMEM39A was found to be bound to seven baits () (28).', '(28) were cell membrane proteins or were predicted to be cell membrane proteins ().', 'The network showing interactions of TMEM39A with its interactors.'] | Fig. 2 The network showing interactions of TMEM39A with its interactors. TMEM39A was found to be interacted with several protein baits ( ). The major function and localization of each interactor were also investigated. Among seven interactor, five proteins including Zacn, Tnfsf13B, IL9R, CHRNA9 and HTR3C are located in cell membrane. Nesprin-4 locates to nuclear outer membrane and BTNL8 is just predicted to be in membrane. Regarding to the functions of those interactors, there are 4 proteins involved in immunology including Tnfsf13B, IL9R, BTNL8, and HTR3C. Other proteins have the following function; Zacn - ion channel, CHRNA9 - neuron development, Nesprin-4 - nuclear positioning. (BTNL8: Butyrophilin-like protein 8, CHRNA9: Neuronal acetylcholine receptor subunit alpha-9, HTR3C: 5-hydroxytryptamine receptor 3C, IL9R: Interleukin-9 receptor, Tnfsf13b: Tumor necrosis factor ligand superfamily member 13B, Zacn: Zinc-activated ligand-gated ion channel). | yes |
PMC6614590 | Figure_1 | oa_package/75/18/PMC6614590.tar.gz | [] | Unlabelled Image | yes |
PMC4011745 | Figure_9 | oa_package/93/a4/PMC4011745.tar.gz | ['Osteoclast (TRAP-positive) cells were detected around the trabeculae in the subchondral bone and necrotic region, with a decreased cell number in the sclerotic region compared to the healthy region ().', 'g009Histology of alkaline phosphatase (ALP) staining.'] | 10.1371/journal.pone.0096361.g009 | yes |
PMC11730535 | Figure_1 | oa_package/d7/f6/PMC11730535.tar.gz | ['Absent validated diagnostic criteria to define disorders associated with pathogenic variation in ELN, phenotype-positive individuals were subjectively defined as participants with one or more major phenotypic findings in one or more systems associated with elastin disorders (bold in 1) or two or more non-major phenotypic findings in two or more systems (normal type in 1).', ' 1Summary of phenotypic findings from chart review of ELN variant carriers(A) Bold text indicates major phenotypic feature.', '0 for either the aortic root or ascending aorta diameter was considered a major cardiovascular phenotypic feature ( 1).', ' 1 shows the type and frequency of phenotypes identified from the chart review.', 'Most involved the aortic root and thoracic aorta and included hypoplasia, dilation, or aneurysm, although aneurysm of the pulmonary artery and aneurysm and/or dissection of medium-sized arteries were also seen ( 1B).', 'Hypoplasia of either the aortic root, ascending aorta, or both was seen in 35 of the 103 phenotype-positive participants based on Z scores 2 of echocardiogram measurements ( 1B), exhibiting a much higher proportions of individuals with abnormal Z scores than the anticipated 2.', 'These percentages represent lower bounds, as the denominator was all phenotype-positive individuals, while the percentages in 1B represent only those with echocardiographic measurements.', 'Beyond the aortic findings, dilation of the pulmonary artery, aneurysm and dissection of medium-sized arteries, arterial tortuosity, and valvar insufficiency were seen ( 1).'] | Figure1 Summary of phenotypic findings from chart review of variant carriers (A) indicates major phenotypic feature. Not used for phenotype scoring. Added after initial PheWAS. Based on GWAS of hernias. Percentages are derived from the number of participants with the specific phenotypic feature divided by all participants with any phenotypic feature ( = 103) multiplied by 100. (B) Percentages of participants with an abnormal aortic root and/or ascending aorta. Forty-two participants had an aortic phenotype out of 87 participants with echo measurements to review (48.2%). The percentages of each aortic phenotype are calculated by the number of participants with that phenotype divided by the total number of participants with an aortic phenotype ( = 42). Not every echo had measurements for both aortic root and ascending aorta. See for additional details. Adapted from Cancer-Associated Comorbidities, by (2004). Retrieved from . | yes |
PMC5299811 | Figure_4 | oa_package/52/b6/PMC5299811.tar.gz | ['Current chest radiography [] showed bilateral reticulonodular opacities, coalescence of nodular shadows, obscuration of vascular markings, and bilateral volume loss indicating ongoing fibrosis.', 'Comparison of current chest X-ray [] with the previous one [] showed no radiological improvement over the last 6 months despite taking two courses of ATD from RNTCP.', 'bilateral reticulonodular opacities, coalescence of nodular shadows, obscuration of vascular markings, and bilateral volume loss indicating ongoing fibrosisWith coalescence of nodules, lung parenchyma became fibrotic with appearance of bilateral conglomerated mass lesion.'] | Figure 4 bilateral reticulonodular opacities, coalescence of nodular shadows, obscuration of vascular markings, and bilateral volume loss indicating ongoing fibrosis | yes |
PMC6169688 | Figure_1 | oa_package/14/06/PMC6169688.tar.gz | ['Chest X-ray revealed moderate-sized bilateral pleural effusions (figure 1).', 'Initial chest X-ray showed moderate-sized bilateral pleural effusions.'] | Figure 1 Initial chestX-ray showed moderate-sized bilateral pleural effusions. | yes |
PMC6167628 | Figure_3 | oa_package/64/bb/PMC6167628.tar.gz | [' 3a).', ' 3b).', '.', ' 3c).'] | Fig. 3. (a)Digital subtraction angiography reveals slow contrast filling of the aneurysmal sac through a short internal iliac neck. (b)An iliac stent-graft of 7cm length, proximal width of 16mm, and distal of 10mm is inserted and successfully deployed. Small endoleak from the common iliac is seen. (c)Post-repair CTA shows absence of contrast enhancement in a fully thrombosed sac | yes |
PMC2740015 | Figure_1 | oa_package/ca/c0/PMC2740015.tar.gz | ['There was appreciable thickening of the descending colon as well as para-aortic lymph nodes measuring 7-10 mm ().', '.'] | Figure 1. CT Scan abdomen with evidence of intussusception at the transverse colon. | yes |
PMC6858263 | Figure_1 | oa_package/25/21/PMC6858263.tar.gz | ['Disease presentation before radiotherapy, which shows a unilateral 5.'] | Figure 1 Disease presentation before radiotherapy, which shows a unilateral 5.0 cm erythematous patch to the right inguinal region devoid of scaling | yes |
PMC5385764 | Figure_4 | oa_package/fc/91/PMC5385764.tar.gz | ['MRI revealed an isolated lobulated cyst-like mass which was isointense to muscle on T1-weighted imaging [A] and homogenously hyperintense on T2-weighted images [B], possessing an internal hypointense septation and scalloping the underlying bony cortex.', ' (A and B)Magnetic resonance imaging of the left tibia.'] | Figure 4 (A and B) Magnetic resonance imaging of the left tibia. T1-weighted axial image (A) of the left mid tibia reveals eroded cortex with adjacent soft tissue mass, which is isointense to the muscle and converts to high-intensity signal on axial T2 (B), with a hypointense septation within (arrow) | yes |
PMC8076442 | Figure_6 | oa_package/4f/47/PMC8076442.tar.gz | [' 6).', 'Short-axis cardiac MR images show dilated left ventricle with increased signal intensity of the myocardium on balanced fast field echo cine sequence (b) with corresponding early (c) and late (d) gadolinium enhancement on short-axis, saturation-recovery prepared gradient echo (c) and short-axis inversion recovery fast gradient echo (d) images, and increased signal intensity on T1 mapping (e) and T2 mapping (f) (arrows), as well as mild pericardial effusion (arrowhead)Table 4Cardiac and pulmonary imaging findings in 37 children with multisystem inflammatory syndrome associated with coronavirus disease 2019 (COVID-19)Cardiac/pulmonary findingsRadiographyEchocardiographyCTCardiac MRIStudy frequency36/37 (97%)35/37 (95%)12/37 (32%)12/37 (32%)Cardiac findings Cardiomegaly20/36 (56%)1/12 (8%)1/12 (8%) Pulmonary congestion/oedema27/36 (75%)8/12 (67%) Impaired ventricular function16/35 (46%)3/12 (25%) Atrioventricular valve insufficiency4/35 (11%) Increased central venous pressure3/35 (9%) Pericardial effusion6/35 (17%)4/12 (33%)5/12 (42%) Coronary artery abnormality5/35 (14%)1/12 (8%) Myocardial oedema7/12 (58%)Lung findings Increased, ill-defined central broncho-vascular markingsa22/36 (61%)5/12 (42%) Peripheral reticular pattern/septal thickening7/36 (19%)9/12 (75%) Ground-glass opacity9/36 (25%)10/12 (83%) Airspace opacity/consolidation10/36 (28%)7/12 (58%) Pleural effusion6/36 (17%)7/12 (58%) Nodular consolidation on CT1/12 (8%)aIncludes bronchial wall thickening, peribronchial cuffingAbdominal findingsTwenty (20/37, 54%) children (7 girls, 13 boys, mean age 7.'] | Fig. 6 Cardiac failure in a 6-month-old boy with positive SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) polymerase chain reaction (PCR) test. Anteroposterior chest radiograph shows cardiomegaly. MRI. Short-axis cardiac MR images show dilated left ventricle with increased signal intensity of the myocardium on balanced fast field echo cine sequence ( ) with corresponding early ( ) and late ( ) gadolinium enhancement on short-axis, saturation-recovery prepared gradient echo ( ) and short-axis inversion recovery fast gradient echo ( ) images, and increased signal intensity on T1 mapping ( ) and T2 mapping ( ) ( ), as well as mild pericardial effusion ( ) | yes |
PMC4489713 | Figure_4 | oa_package/a0/5e/PMC4489713.tar.gz | ['In comparison to the WT mice, the VCPR155H/+ mice on increasing LED displayed comparable levels of ubiquitin, however, decreased levels of LC3-I and LC3-II protein expressions, as well as reduced levels in p62/SQSTM1 protein (D and 4E).', 'Interestingly, magnified insets revealed increased p62 puncta aggregates in the heterozygous VCPR155H/+ mice on the 30% and 48% LED, suggesting increased autophagy dysregulation, even though there was decreased levels of p62 overall (A 4C, magnified insets).', 'Furthermore, we examined the TDP-43 aggregates (nuclear to cytoplasmic translocation) in the VCPR155H/+ animals versus their WT littermates and showed nuclear TDP-43 expression, suggestive of a reduced pathological phenotype (A 4C, indicated by white arrows).', 'Western blot and densitometry analyses of ubiquitin, LC3-I/II, p62/SQSTM1, and TDP-43 confirmed these findings (D and 4E).', 'g004Autophagy influx in the quadriceps of WT and VCPR155H/+ mice on normal and different lipid-enriched diets.'] | 10.1371/journal.pone.0131995.g004 | yes |
PMC8820480 | Figure_3 | oa_package/6b/f3/PMC8820480.tar.gz | ['A: A tumor nodule is seen under the squamous epithelium in the cervical biopsy specimen (H E) (x40).'] | Figure 3 A: A tumor nodule is seen under the squamous epithelium in the cervical biopsy specimen (H&E) (x40). B: A high-power view of the solid tumor with atypical cellular features is seen (H&E) (x200). C: Immunohistochemically, the tumor cells show diffuse nuclear staining with WT-1 (x100). D: Immunohistochemically, the tumor cells show diffuse nuclear staining with PAX-8 (x100). | yes |
PMC9396697 | Figure_2 | oa_package/70/17/PMC9396697.tar.gz | ['.'] | Figure 2. Benign breast ducts at the bottom of the picture show strong positive ER staining, while the tumor cells show weak patchy staining. | yes |
PMC9407377 | Figure_8 | oa_package/af/a2/PMC9407377.tar.gz | ['A number of skin lesions are diagnostic for TS, including facial angiofibromas (malar hamartomatous red nodules) (A), hypopigmented macules ( ash leaf spots and confetti lesions), shagreen patches (grayish-green/ light brown lesions in the lumbosacral region), and periungual fibromas ( Koenen tumors , soft periungual nodules) [2,15,45,46].', 'RMLs, extending outward from the ventricular surface toward the cortex, appear as curvilinear or straight T2/FLAIR hyperintensities on MRI (B).', 'Peripheral linear signal loss, termed India ink artifact, is seen on T1-weighted opposed-phase images (C) [46,47].', 'LAM almost exclusively occurs in women, and is characterized by diffuse, thin-walled, well-circumscribed lung cysts of varying sizes and uniform distribution in bilateral lungs (D).', 'Dermatologic and radiologic images characteristic of tuberous sclerosis (TS): (A) Confluent small angiomatous (erythematous, glistening) papules on the cheek and nose of a 44-year-old man consistent with neurofibromas of TS.'] | Figure 8 Dermatologic and radiologic images characteristic of tuberous sclerosis (TS): ( ) Confluent small angiomatous (erythematous, glistening) papules on the cheek and nose of a 44-year-old man consistent with neurofibromas of TS. These lesions were not present during the first few years of life. ( ) Axial FLAIR MRI of the brain shows linear hyperintensity extending radially from the left subcortical white matter to the graywhite junction, representing subependymal tubers (arrow). ( ) Coronal T1WI of the abdomen demonstrating a large fat-containing right renal mass, representing angiomyolipoma (arrow). ( ) Coronal HRCT of the chest demonstrates innumerable thin-walled cysts in a diffuse distribution and a right pneumothorax. Findings are consistent with lymphangioleiomyomatosis (LAM) with spontaneous pneumothorax. | yes |
PMC9664609 | Figure_3 | oa_package/4f/59/PMC9664609.tar.gz | [' 3F.', '3F.', 'F Control muscle from a child without muscle disease demonstrating a normal mosaic pattern of three different fiber types: Type 1 fibers with slow/beta cardiac MyHC: blue, type 2A fibers with MyHC IIa: green, type 2B fibers with MyHC IIx: red and perlecan for basement membranes: yellowIn case II:2 a muscle biopsy at age 55 years from the vastus lateralis muscle revealed marked variability of fiber size with many fibers larger than normal and many atrophic or hypoplastic fibers (', '3A).', '3B).', '3C).', '3D, E).', '3F.'] | Fig. 3 Muscle biopsy from the vastus lateralis muscle in case II:2 at age 55years. The majority of muscle fibers are larger than normal and many of them show internal nuclei. There are also scattered small and very small fibers, and fatty replacement of muscle fibers (H&E). The internal structure of the large fibers is slightly disturbed (NADH-TR). Scattered regenerating fibers (arrow) (Immunostaining of embryonic MyHC). - Immunostaining of MyHC isoforms and perlecan. For normal control, see . There is nearly type 1 fiber (blue) uniformity with a few scattered very small type 2A fibers (green, arrows). There are no type 2B fibers. High magnification shows numerous extremely small type 2A fibers (arrows). Control muscle from a child without muscle disease demonstrating a normal mosaic pattern of three different fiber types: Type 1 fibers with slow/beta cardiac MyHC: blue, type 2A fibers with MyHC IIa: green, type 2B fibers with MyHC IIx: red and perlecan for basement membranes: yellow | yes |
PMC10920925 | Figure_5 | oa_package/ae/e9/PMC10920925.tar.gz | ['74 year old female with recurrent endometrial carcinoma with vaginal metastases.'] | Figure 5 74yearold female with recurrent endometrial carcinoma with vaginal metastases. GTV shown around the vaginal nodule on MRI (B), otherwise not seen on CT (A). | yes |
PMC9641849 | Figure_2 | oa_package/da/1b/PMC9641849.tar.gz | [' 2a, b).', ' 2c, d).', 'pylori in mouse gastric mucosa was not decreasedZZDX treatment decreased the expression levels of inflammatory factors induced by H.'] | Fig. 2 ZZDX treatment did not decrease colonization in mouse gastric mucosa. immunohistochemical staining showed that compared with the NC group ( ), was successfully colonized in the HP group ( ). After ZZDX treatment at either the low dose ( ) or the high dose ( ), the colonization of in mouse gastric mucosa was not decreased | yes |
PMC5534902 | Figure_2 | oa_package/9c/9b/PMC5534902.tar.gz | ['MRI T2 image (frontal view) sellar/suprasellar mass that was isohyperintense with peripheral hyperintensityMRI T1 image (sagittal view) non-contrast fat saturated showing fluid levelTherefore, the conclusion was suggestive of macroadenoma of pituitary gland (size 1.'] | Figure 2 MRI T2 image (frontal view) sellar/suprasellar mass that was isohyperintense with peripheral hyperintensity | yes |
PMC11328004 | Figure_367 | oa_package/e2/f1/PMC11328004.tar.gz | [] | Chart 302 Structure of Complex | yes |
PMC4988036 | Figure_1 | oa_package/e3/b7/PMC4988036.tar.gz | ['Comparison between the groups ( 1) showed that TBX21 was significantly increased in paucibacillary sheep, resulting in a 2.', 'There were no significant differences between the groups with GATA3v1 ( 1C).', ' 1\nRelative quantification of\nA\nTBX21, B\nGATA3\nand\nC\nGATA3v1\nin ICLN of paratuberculosis-diseased and uninfected control sheep.', 'RORC2 expressionQuantification of two RORC2 variants showed overall significant differences (Table 2) for both the full length transcript (RORC2, P = 0.'] | Figure1 , Each point is the mean Cq of three biological replicates per animal, each in duplicate. ANOVA: , =0.003; , =0.046; , =0.32; error bars,SD. *0.05, >0.01; **0.01, 0.001; ***0.001. | yes |
PMC11093900 | Figure_1 | oa_package/1d/12/PMC11093900.tar.gz | ['(A, B) Computed tomography (CT) abdomen and pelvis obtained upon admission, coronal and axial views, respectively.'] | Figure 1 (A, B) Computed tomography (CT) abdomen and pelvis obtained upon admission, coronal and axial views, respectively. Evidence of severe pancreatitis with walledoff necrosis in the bilateral paracolic gutters extending to the midpelvis. (C,D) CT abdomen and pelvis obtained after discharge (53 days after the CT depicted in Images [A, B]) from the hospital. Significant interval improvement in both pancreatitis and in the size of the walledoff collection is shown. | yes |
PMC7739559 | Figure_10 | oa_package/c2/96/PMC7739559.tar.gz | [] | Fig. 10 Pigmented iris lesion | yes |
PMC3796764 | Figure_3 | oa_package/64/aa/PMC3796764.tar.gz | ['Using leukocyte lineage (Lin) markers CD11b, GR1 and B220, we found that cytokine expressing cells were CD45+Lin and distinct from common innate cell populations (Supplementary ).', 'Thy1hiSCA-1+ innate lymphoid cells were present at low frequency in the Rag / colon but increased significantly during intestinal inflammation (a).', 'hepaticus infected mice and following IL-23 stimulation suggesting not only accumulation, but also activation of this population in the inflamed intestine (b).', 'Efficient depletion of Thy1+ cells led to abrogation of both colitis and typhlitis (c and e).', 'IL-23 dependent systemic immune activation was also ablated as shown by the significantly reduced splenomegaly (d).', 'Absence of intestinal inflammation following depletion of Thy1+SCA-1+ correlated with abrogation of both spontaneous and IL-23-induced IL-17 and IFN- production by cLP cells (f).', 'Thy1hi innate lymphoid cells drive H.'] | Figure 3 Thy1 innate lymphoid cells drive -induced innate intestinal inflammation. , Frequency of Thy1 SCA-1 cells (n=3-6) and , IL-17 expression among Thy1 cells following culture with or without IL-23 in cLP cells from control or infected 129SvEv mice. Data are representative of 2 independent experiments. Colitis and typhlitis scores ( ), splenomegaly ( and representative photomicrographs (50; scale bars 200m) ( ) from infected mice treated with -Thy1 or isotype (Iso) control mAbs (n=6 per group). , IL-17 and IFN- secretion by cLP cells from the mice described above, following stimulation with or without IL-23. Data represents mean s.e.m. (n=6). *P<0.05; ** P<0.01. | yes |
PMC2622762 | Figure_1 | oa_package/2f/fb/PMC2622762.tar.gz | ['0004262-Hosoya1" ref-type="bibr">[8] (A).', 'Repo is expressed from glioblasts, immature to mature glial cells but not in neuroglioblasts (B).', 'g001Expression patterns of gcm and repo genes during glial cell lineage differentiation.', 'g001"/>In , such differences between gcm and repo expression patterns are summarized.', 'Therefore, in brief, we can induce glial cell-specific gene expression by repo driver and stem/progenitor cell-specific gene expression by gcm driver in the glial cell lineage (A, B).', 'Existence of different types of cells in glial cell lineage or neuronal cell lineage is also summarized in Table (C).', 'In the case of hHtt, gcm-driven expression, either in neuroglioblasts or gliobalsts, before L1 stage during development () permitted development to pupae and adult flies although the number of adult flies was reduced (Table 1).'] | 10.1371/journal.pone.0004262.g001 | yes |
PMC8354388 | Figure_3 | oa_package/0c/f8/PMC8354388.tar.gz | ['Based on the previous studies on SARS-CoV and other viruses, the mechanism of IVM in COVID-19 was proposed to be inhibition of signal-dependent nucleocytoplasmic shuttling of the SARS-CoV nucleocapsid protein by an inhibitory effect on importins / [].', '[31686970717273105106107108109110111112113114115](a) IVM binds importin (IMP) armadillo (ARM) repeat domain causing thermal instability and helicity thus preventing IMP -IMP 1 interaction.', 'CD147 is prominently expressed on red and white blood cells apart from the sites of expression of ACE2 receptorTable 2Possible Anti-Sars-Cov-2 mechanisms of ivermectin[2731979899100101102103104]Importin / heterodimer-prevention of heterodimer formation; dissociation of formed heterodimer []Shields SARS-CoV-2 spike protein preventing its binding to the CD147 transmembrane receptor and ACE2Allosteric modification of 7 nACh Receptor-(important component of the Nicotinic hypothesis )Inhibition of SARS-CoV-2 RNA dependent RNA polymerasePositive allosteric modulation of P2X4 receptorAnti-inflammatory and immunomodulatory effects: downregulates gene expression of pro-inflammatory molecules-IL-8, TNF- , and cathelicidinLL-37Prevention of Vitamin D receptor nuclear entry by inhibiting importin-4-resultant anti-inflammatory effects mainly mediated by downregulated LL-37 expressionIonophore roleTable 3Studies conducted so far on the clinical utility of ivermectin as therapeutic as well as prophylactic regimen[30686970717273105106107108109110111112113114115]AuthorStudy Outcome\n\n\nIn vitro study of IVM in COVID -19\nCaly et al.'] | Figure 3 (a) IVM binds importin (IMP) armadillo (ARM) repeat domain causing thermal instability and helicity thus preventing IMP-IMP1 interaction. Further, it can also dissociate IMP-IMP1 heterodimers. This leads to impaired SARS-CoV-2 protein translocation into the nucleus via nuclear pore complex. An efficient nuclear transport of SARS-CoV-2 is essential for virus replication and downregulation of host immune response. (b) A second proposed mechanism is shielding of the SARS-CoV-2 spike protein thus preventing its binding to ACE-2 receptor and CD 147 receptor (basigin/EMMPRIN). ACE-2, a homologue of ACE, is an important cell surface receptor, which mediates SARS-CoV-2 infection by recognition of spike protein. The interaction with CD 147 has been recently recognized. CD147 is prominently expressed on red and white blood cells apart from the sites of expression of ACE2 receptor | yes |
PMC5009300 | Figure_7 | oa_package/4a/8b/PMC5009300.tar.gz | ['AS-IV treatment suppressed the activation of these proteins ().', 'org/1999/xlink" xlink:href="srep32545-f6"/>Mice in the db/db group showed more renal cortical expression of p-Akt(Ser473), p-mTOR(Ser2448), p-NF- B p65(Ser536) and p-Erk1/2(Thr202/Tyr204) than wild type mice, as indicated by Western blot respectively.', 'AS-IV treatment suppressed activation of these proteins ().'] | Figure 7 Mice in the db/db group showed more renal cortical expression of p-Akt(Ser473), p-mTOR(Ser2448), p-NF-B p65(Ser536) and p-Erk1/2(Thr202/Tyr204) than wild type mice, as indicated by Western blot respectively. AS-IV treatment suppressed activation of these proteins ( ). n=49 per group. * <0.05, ** <0.01 and *** <0.001 relative to the wild type group. <0.05 and <0.01 relative to the db/db group. | yes |
PMC10881762 | Figure_1 | oa_package/eb/f8/PMC10881762.tar.gz | [' 1A; for summary of all PCR array results see Supporting ', ' 1A).', ' 1A).', ' 1B).', ' 1C; for p values see Supplemental Table 1C).', 'Upregulation of VEGF and VEGFR in fast-flow malformations.', 'Means and standard deviations are shownBevacizumab and thalidomide mitigate VEGF induced effects in endothelial AVM cellsOur results demonstrated that mechanical stress led to an upregulation of VEGF and TGF- in the microenvironment of AVMs.'] | Fig. 1 Upregulation of VEGF and VEGFR in fast-flow malformations. PCR array analysis of angiogenesis-associated genes of fast-flow (n=10; Group1) and slow-flow (n=10; Control Group) patients shows an upregulation of VEGF (VEGFA, VEGFB, VEGFC) and VEGF-receptors (VEGFR-1=FLT1, VEGFR-2=KDR, VEGF co-receptors NRP-1 and NRP-2), as well as an upregulation of angiogenic signaling molecules FGF-1, FGF-2, TGF- 1 and their receptors (TGF- receptor 1 and FGFR 3) in fast-flow malformations. Scatter plot shows fold change, which is the normalized (2Ct) gene expression in fast-flow lesions (Group 1) divided by the normalized gene expression in slow-flow lesions (Group 2). Upregulated genes are highlighted red. Beta-actin (ACTB) functions as housekeeping gene. Immunohistochemical staining of fast-flow (n(AVM)=10) and slow-flow (n(VM)=6; n(LM)=4) malformations reveals an upregulation of VEGFR-2 and TGF- 1 in the microenvironment of fast-flow malformations. The H-score assesses the extent of immunoreactivity of the VEGF receptor staining. P-values displayed were calculated by Mann Whitney test. Means and standard deviations are shown. HPF=high-power fields; SF=slow flow malformation; FF=fast flow malformation. Scale bar, 100m Exposed to cyclic mechanical stretching (CMS), AVM CD31 endothelial cells show an upregulation of VEGF on mRNA and protein levels after 24h and 48h (for all p-values see Supplemental Table 1C). Human endothelial cells (HDMEC) and fibroblasts (NHF) do not respond to mechanical stress after 48h. P-values displayed were calculated by one-way ANOVA followed by the post hoc idk test for multiple comparisons. Means and standard deviations are shown | yes |
PMC6642321 | Figure_9 | oa_package/41/bc/PMC6642321.tar.gz | ['Human BM MSC derived EVs repair kidney injury in Cd exposed medaka.'] | Figure 9 Human BMMSCderived EVs repair kidney injury in Cdexposed medaka. (A) LTL labeled the PT apical membrane (green). ExoGlowmembrane red dye labeled hBMMSC EVs (red). DAPI was used to stain nuclei (blue). (B) LTL labeled the PT apical membrane (green). LTL (green), DAPI (blue), and a5 labeled the Na , K ATPase alpha subunit basolateral localization (red). (C) PAS staining of JB4 sections in the kidneys. (D) HE staining of JB4 sections in the kidney. (E) Fluorescence nanoparticle analysis. Finite Track Length Adjustment (FTLA), FTLA measurement for light scattering (LSFTLA). Regular size/concentration measurement for light scattering (LSSC), FTLA measurement for fluorescent data (FFTLA), regular size/concentration measurement for fluorescent data (FSC). (F) Survival proportion graph of 7 dai female medaka exposed to Cd and treated with different preparations of BMMSC EVs. Significance by Logrank test. ***** < 0.0001; ns=not significant. The white scale bar indicates 50m (A, B). The black scale bar indicates 50m (C, D). | yes |
PMC8765042 | Figure_4 | oa_package/78/06/PMC8765042.tar.gz | ['Finally, to confirm whether the observed glomerular structural alterations can also be found in mouse or human renal histological sections, we performed histological staining of mouse kidney tissue and renal biopsy specimens from patients with AS (, A and B).', 'Glomerular capillary distension was not observed in histological paraffin sections of the same mice that showed the phenotype of capillary aberrations in vivo with MPM (A).', ', from routine diagnostic biopsies) detected the presence of CD3+ and CD44+ immune cells in glomeruli; however, distended capillaries were rarely seen (B).', 'Histological features of mouse and human kidney tissue AS sections.'] | Figure 4 Histological features of mouse and human kidney tissue AS sections. ( ) Periodic acidSchiffstained (PAS-stained) thin sections of the kidney tissue from same mice that were used for intravital imaging. Note the lack of severely distended glomerular capillaries in either control or AS tissues. ( ) Representative semithin section with toluidine blue staining (top) of 5 of 34 samples from patients with AS and a paraffin section with immunofluorescence multilabeling (bottom) for CD31 (green), CD44 (purple) and CD3 (red). Note the presence of distended glomerular capillaries (arrowheads) and CD44 and CD3 immune cells in the glomerular capillary lumen (red and purple arrows, respectively) in these specific human AS sections. Nuclei are stained blue with DAPI. Scale bars: 20 m (all images). | yes |
PMC3674754 | Figure_3 | oa_package/52/21/PMC3674754.tar.gz | ['The right eye exhibited a right lower lid cicatricial entropion with associated palpebral conjunctival cicatrix (), trichiasis, and breakdown of the corneal epithelium.', 'Patient showing a significant difference in clinical appearance of the right and left eyes four months after disease onset.'] | Figure 3 Right lower fornix of the patient five weeks after disease onset. Evident is a contracted tarsal conjunctival membrane (arrow) resulting in cicatricial entropion, trichiasis, and corneal epithelial breakdown. | yes |
PMC8588800 | Figure_4 | oa_package/ed/b5/PMC8588800.tar.gz | ['As shown in \n4A, two different siRNAs were used to knockdown JAK1, which significantly reduced\nDEA/NO-induced IL-6, CCL2, CCL20, CXCL1, and CXCL2.', 'We measured IL-6 by ELISA to confirm\nthese effects at the protein level (\n4B).', 'Additionally, we found that the effect on IL-6 was JAK1 specific as knockdown\nof JAK2 had no effect on IL-6 expression (C).', '.', '(B)\nJAK1 was knocked down as in \n4A.', '1177_17590914211033650-fig4" position="float"/>Abbreviations: JAK1 = Janus kinase-1; JAK2 = Janus kinase-2; NO = nitric oxide;\nsiRNA = small interfering RNA; ELISA = enzyme-linked immunosorbent assay;\nDEA/NO = diethylamine NONOate; qPCR = quantitative polymerase chain reaction;\nANOVA = analysis of variance.', 'While JAK1 knockdown reduced\nDEA/NO-induced CXCL1 and CXCL2 expression by 50% (A), JAK inhibition had no effect on the\ninduction of these chemokines (\n5C).'] | Figure 4. JAK1 is required for the NO-induced inflammatory response. (A) Astrocytes weretransfected with control (Ctl) siRNA or two different siRNA targeting JAK1. The cellswere then treated DEA/NO (1mM) for 4h followed by analysis of gene expression. (B)JAK1 was knocked down as in . Cells were treated with DEA/NO (1mM) for 24h and the cell culture mediawas collected to measure IL-6 by ELISA. (C) Astrocytes were transfected with Ctl siRNA,JAK1 (#2), or JAK2 siRNA. Cells were treated with DEA/NO (1mM) for 4h followed byqPCR. Data are the meansstandard deviation, =34 independentexperiments. * .05 by two-way ANOVA with Bonferroni's multiplecomparisons test. | yes |
PMC5450388 | Figure_1 | oa_package/ae/a1/PMC5450388.tar.gz | [' 1).', 'Atherosclerotic plaque formation.', '01\nVascular calcification.'] | Fig. 1 Atherosclerotic plaque formation. Evaluation of aortic plaque formation in male and female or mice. Exemplary en face preparations of aortae of male and female or mice, stained with Sudan IV ( ) or unstained ( ). Exemplary PAS-stained cross sections of the brachiocephalic trunk of male and female or mice. * <0.05; ** <0.01 | yes |
PMC5735134 | Figure_6 | oa_package/bc/65/PMC5735134.tar.gz | [' 6B), an expansion of ASF 361 upon removal of ASF 356 or ASF 457 (', ' 6C and D), and, as seen in our previous experiments, decreased numbers of ASF 492 following DSS treatment regardless of the community composition (', ' 6E).', 'Removal of immune-dominant gut symbionts did not create patterns of microbial community structure that were unique to pathobiont-induced disease.', '\nDiscussionIBD is hypothesized to result from aberrant immune responses directed against gut symbionts, which can be initiated by the loss of intestinal barrier integrity24 26.'] | Figure 6 Removal of immune-dominant gut symbionts did not create patterns of microbial community structure that were unique to pathobiont-induced disease. ( ) ASF bacterial abundances in cecal contents of animals colonized with all ASF members or devoid of the following taxa: ASF 356 ( .), 356 and 361 ( ), or 457 ( ). Mice harboring these communities were then colonized with for 3 wks and then either treated with 1.5% DSS or left untreated at 1213 wks of age ( =1011 animals per treatment). Asterisks depict the degree of significance for differences as determined by a non-parametric unpaired Mann-Whitney test using a two-tailed distribution for -value calculations (* <0.05, ** 0.01, *** 0.001, **** 0.0001 and NS=not significant 0.05). Differing superscript letters indicate significant differences across treatments as per a non-parametric Kruskal-Wallis one-way ANOVA, followed by a post-hoc test (Dunns test, <0.05). ND refers to not detectable. ASF bacterial abundances were measured using species-specific qPCR assays. Experiments were initiated when male and female C3H/HeN mice were 45 wks of age. | yes |
PMC7028037 | Figure_3 | oa_package/28/3e/PMC7028037.tar.gz | ['(D,E,F): after the treatmentCT scans and bronchoscopy images of LAM before and after the comprehensive treatment.'] | Figure 3 CT scans and bronchoscopy images of LAM before and after the comprehensive treatment. (A,B,C): before the treatment. (D,E,F): after the treatment | yes |
PMC7653184 | Figure_9 | oa_package/0e/04/PMC7653184.tar.gz | ['\nRetrograde urethrocystogram.'] | Figure 9 A and B: Retrograde urethrocystogram showing significant stricture of the bulbar urethra. Post-urethroplasty fluoroscopic examination showing focal extravasation of contrast (orange arrow). Foley catheter was therefore not removed. | yes |
PMC8956716 | Figure_12 | oa_package/98/76/PMC8956716.tar.gz | [] | Figure 12 Partition distribution of annotated lymph nodes. | yes |
PMC11273058 | Figure_1 | oa_package/3e/bd/PMC11273058.tar.gz | ['F1">.'] | Fig. 1. . Figure summarising the development and progression of the atherosclerotic plaque, which obstructs the coronary lumen and if unstablecan rupture, with subsueqent thrombus formation occluding the affected coronary arteryresulting in acute coronary syndromes. LDL, low density lipoprotein; VCAM1, vascular cell adhesion molecule 1; CCL2, chemoattractant protein 1; ROS, reactive oxygen species; LDLox, oxidised LDL; IFN-Y, interferon gamma; TNF- , tumour necrosis factor alpha; IL-2, interleukin 2; IL-3, interleukin 3; IL-10, interleukin 10; TGF , transforming growth factor beta; Th1, T helper type 1; , regulatory T cell. | yes |
PMC7185769 | Figure_13 | oa_package/f2/8f/PMC7185769.tar.gz | [] | Fig.13 Severe anemia. | yes |
PMC6158386 | Figure_2 | oa_package/b7/7c/PMC6158386.tar.gz | [] | FIGURE 2 mRNA expression of genes encoding for Creb1, neurotrophins (Bdnf, Ngf, and Ntf3) and their receptors (Ntrk2, Ntrk3) in the hippocampus of saline- and fluoxetine-treated wild-type, Creb1 , and Creb1 Crem/ mice. Neither genotype nor fluoxetine influenced the mRNA expression of Creb1, Bdnf, Ngf, Ntf3, Ntrk2, and Ntrk3. Bars represent fold changes in the mRNA expression of Creb1, Bdnf, Ngf, Ntf3, Ntrk2, and Ntrk3 vs. that in wild-type non-treated animals (dot line) in the hippocampus of wild-type fluoxetine-treated, Creb1 saline- and fluoxetine-treated, and Creb1 Crem/ saline- and fluoxetine-treated mice. All graphs show data from males (left) and females (right). W/t, wild-type; FLX, fluoxetine. Data are presented as the mean SEM. | yes |
PMC10590186 | Figure_2 | oa_package/de/45/PMC10590186.tar.gz | ['28%) ().', 'The PAS stain was used to confirm the presence of fungal infection in all the cases that were positive in the\nhistopathology test ().', '90%) showed the presence of septate hyphae with dichotomous branching in addition\nto broad aseptate hyphae in histopathology ().', 'tif"> A) Hematoxylin and Eosin (H E) stained sections show numerous broad aseptate fungal hyphae of Mucormycosis (100x).'] | Figure 2 A) Hematoxylin and Eosin (H&E) stained sections show numerous broad aseptate fungal hyphae of Mucormycosis (100x). B) Periodic acid-Schiff-stained sections show fungal hypha in dark pink color. | yes |
PMC7702158 | Figure_3 | oa_package/c5/e9/PMC7702158.tar.gz | ['ADAM10 is upregulated in the kidneys of CPF exposed mice.'] | Figure 3 ADAM10 is upregulated in the kidneys of CPFexposed mice. (A) Relative expression trends of , , and in GeneChip data from E12.5 to E18.5 normal embryonic kidneys. (B) Comparision of the mRNA levels of in E18.5 and 4 and 8week kidneys between the two groups ( =6). (C, D) Effect of prenatal CPF exposure on the protein levels of ADAM10 in E18.5 and 4 and 8week kidneys. =6. (E) E18.5 immunofluorescence staining with antibodies against LTL (green) and ADAM10 (red). Scale bars: 100m. (F) Costaining for ADAM10 (red) and presenilin1 (PS1) (green) at E18.5 kidneys. Quantification data are shown in panels G and H. =6. Scale bars: 50m. (I) Costaining for HES1 (red) and PS1 (green) at E18.5 kidneys. Quantification data are shown in panels J and K. =6. Scale bars: 50m. MeanSD from three to five experiments. **** <0.0001, *** <0.001, ** <0.01, * <0.05. | yes |
PMC9583561 | Figure_5 | oa_package/88/cd/PMC9583561.tar.gz | [' 5), compared with patients who only received radiotherapy, surgery plus radiation increased CSS (p = 0.', '570aincluding: American Indian/AK Native, Asian/Pacific Islanderbincluding: mandible/skull, face and associated joints/rib, sternum, clavicle and associated jointscincluding: divorced, separated, widowedThe graphs revealed Kaplan-Meier curves between the following groups (data from the propensity score sample): A-B surgery + radiotherapy vs.', '002)DiscussionUMBTs are much less common than the specific bone malignancies, including osteosarcoma, chondrosarcoma, and Ewing sarcoma, etc.'] | Fig. 5 The graphs revealed Kaplan-Meier curves between the following groups (data from the propensity score sample): surgery + radiotherapy vs. radiotherapy for cancer-specific ( =0.003) and overall death rate ( =0.002); surgery + chemotherapy vs. chemotherapy for cancer-specific ( =0.035) and overall death rate ( =0.002) | yes |
PMC9513936 | Figure_3 | oa_package/04/bc/PMC9513936.tar.gz | [' 3E, F).', 'Dark vs typical microglia s interactions with parenchymal elements.', 'Red outline = plasma membrane, yellow outline = nuclear membrane, blue outline = basement membrane, ma = myelinated axons, A = axon terminals, S = dendritic spines, orange asterisk = mitochondria, green asterisk = altered mitochondria, blue asterisk = endoplasmic reticulum, red arrow = Golgi apparatus, lb = lipid body, 3rd = tertiary lysosome, 2nd = secondary lysosome, Lg = lipofuscin granules, pink pseudo-coloring = dystrophic neuritesTable 1Absolute ultrastructural analysis of dark microglia near A plaques and dystrophic neurites compared to typical microglia near vs far A plaques and dystrophic neurites in the ventral hippocampus CA1 stratum lacunosum-moleculare of aged-matched APP-PS1 vs C57BL/6J male miceC57BL/6JAPP-PS1CTMMean SEM(Min Max)FTMMean SEM(Min Max)NTMMean SEM(Min Max)NDMMean SEM(Min Max)Area ( m2)26.', ' 3H, I).', ' 3G).'] | Fig. 3 Dark vs typical microglias interactions with parenchymal elements. Representative 5nm resolution scanning electron microscopy images taken in the ventral hippocampus CA1 of 20-month-old APP-PS1 and C57BL/6J male mice. Typical microglia (TM) in C57BL/6J mice contacting a blood vessel (labeled BV) and myelinated axons (labeled ma) as well as axon terminals (labeled A), TM far from a plaque interacting with axon terminals and dendritic spines (labeled S), TM near a plaque interacting with a few axon terminals, dark microglia (DM) near a plaque is contacting axon terminals. Graphs representing the number of axon terminals ( ), all synaptic interactions ( ), percentage of cells associating with the vasculature ( ), myelinated axons ( ), as well as the percentage of cells touching a myelinated axon ( ). Data shown are expressed as meansS.E.M. * p<0.05, ** p<0.01, using a KruskalWallis test with a Dunns multiple comparisons post hoc test. Statistical tests were performed on n=912 microglia per animal with N=3 mice/group, for a total of 111 microglial cell bodies analyzed. Red outline=plasma membrane, yellow outline=nuclear membrane, blue outline=basement membrane, ma=myelinated axons, A=axon terminals, S=dendritic spines, orange asterisk=mitochondria, green asterisk=altered mitochondria, blue asterisk=endoplasmic reticulum, red arrow=Golgi apparatus, lb=lipid body, 3 =tertiary lysosome, 2nd=secondary lysosome, Lg=lipofuscin granules, pink pseudo-coloring=dystrophic neurites | yes |
PMC8721135 | Figure_2 | oa_package/94/82/PMC8721135.tar.gz | ['verruca, knuckle pads, papular granuloma annulare), evaluating these lesions alongside the proximal nail folds and cuticles is key ().', 'Dermoscopic image of nailfold capillary dilation alternating with dropout, focal hemorrhage, and cuticular dystrophy.', "Ulcers overlying Gottron's papules are highly concerning for a subtype of DM associated with the anti-melanoma differentiation-associated gene 5 (MDA5) autoantibody."] | Fig. 2 Dermoscopic image of nailfold capillary dilation alternating with dropout, focal hemorrhage, and cuticular dystrophy. | yes |
PMC6839082 | Figure_3 | oa_package/dc/f2/PMC6839082.tar.gz | [' 3b), nuclear depletion of TDP-43 (', ' 3c), and punctate nuclear TDP-43 deposition (', ' 3d).', ' 3e).', 'Scale bar = 20 mTo determine if loss of motor neurons was occurring as previously described, motor neurons in the ventral horn of spinal cords were counted across a minimum of 4 separate whole lumbar spinal cord sections in 3 animals per group.', ' 3b, d) was positive, and the absence of all of these was considered negative.', ' 3c) to avoid potential over-estimation of the pathology.', ' 3a).'] | Fig. 3 Spinal cord TDP-43 pathology in ChAT-tTA/TRE-TDP-43 rats was detected by immunohistochemistry with anti-TDP-43 antibodies regardless of hippocampal injected tau construct. Frequency of pathology-bearing motor neurons normalized against total number of motor neurons. The presence of spinal TDP-43 pathology approaches significance in the rats expressing tau when compared to GFP expressing rats ( <0.1). MN=motor neuron, GFP=GFP only control, tau =GFP-tagged tau protein construct, tau =GFP-tagged tau protein construct). This was not significant by one-way ANOVA ( =0.052). Photomicrograph showing spinal cord dorsal horn TDP-43 cytosolic inclusion in a motor neuron. Photomicrograph of a spinal motor neuron showing depleted nuclear TDP-43 with cytosolic TDP-43 and surrounding glial cells. Photomicrograph of spinal motor neurons exhibiting punctate TDP-43 pathology. Confocal imaging detecting cleaved caspase-3 (green) in a motor neuron co-expressing human TDP-43 (red) punctate pathology. This was observed in all ChAT-tTA/TRE-TDP-43 rats where TDP-43 displayed pathology regardless of hippocampal tau expression ( =3 for all groups). Scale bar=20m | yes |
PMC7086115 | Figure_1 | oa_package/2f/de/PMC7086115.tar.gz | ['Duodenal bulb (black arrow) Prepyloric deformity (red arrow) Second portion of duodenum with ulcer (black arrow) and visible vessel (red arrow) Epinephrine could not be injected due to hard and fibrotic tissue around the duodenal stenosis.'] | Figure 1 Duodenal bulb (black arrow) | yes |
PMC11595037 | Figure_3 | oa_package/43/8b/PMC11595037.tar.gz | ['The number of both astrocytes and FJB+ astrocytes increased with days of culture (A C).', '2%) (D).', 'Similarly, an increase in the number of FJB+ astrocytes correlated with prolonged culture time (), which was consistent with a previous report [34].', 'The number of RFs increased with prolonged culture.'] | Figure 3 The number of RFs increased with prolonged culture. ( ) Representative images of DAPI, GFAP, and FJB staining in cells after different incubation times. ( ) The number of GFAP cells per a field of view with culture time. D7: in 7 field of views from 7 wells: D11: in 8 field of views from 8 wells: D17: in 2 field of views from 2 wells. The data are presented as the mean SEM. D7 vs. D11: *** = 3.6 10 , one-way AVOVA with Bonferroni correction. ( ) The number of FJB astrocytes per a field of view with culture time. D7: in 7 field of views from 7 wells; D11: in 8 field of views from 8 wells; D17: in 2 field of views from 2 wells. The data are presented as the mean SEM. D7 vs. D11: *** = 9.4 10 , one-way AVOVA with Bonferroni correction. ( ) Changes in the percentage of FJB /GFAP cells per field of view with culture time. D7: in 7 field of views from 7 wells, 16.7%; D11: in 8 field of views from 8 wells, 28.2%; D17: in 2 field of views from 2 wells, 41.2%. The data are presented as the mean SEM. D7 vs. D11: * = 0.017, one-way ANOVA with Bonferroni correction. | yes |
PMC9462500 | Figure_4 | oa_package/25/48/PMC9462500.tar.gz | ['The mice were kept for an additional 2 weeks at 7% oxygen level followed by various functional and structural analyses (A).', 'As shown in B, UCP2 protein levels increased in response to hypoxia in the WT mice, confirming the in vitro results in the cell models.', 'Additionally, the increase in UCP2 after hypoxia seems to be more evident in CM than nonmyocytes, suggesting the observed phenotype may in part be due to the loss of UCP2 in CM (Supplemental , A and B).', 'Interestingly, mice lacking UCP2 have significantly decreased ability to survive in a hypoxic environment compared with their WT littermates (Supplemental C).', 'Further echocardiography analysis revealed an overall decrease in systolic function as measured by analysis of ejection fraction (EF; C) and fractional shortening (FS; D and Supplemental D) in both males and females from the UCP2KO group when compared with the WT 4 weeks after moderate hypoxia, while no difference was observed for the WT group.', 'Heart rate (Supplemental E) did not change between both groups of animals.', 'Additionally, speckle-tracking based strain analysis on echocardiography B-mode loops was performed, and E shows 3-dimensional regional wall velocity diagrams and vector diagrams for both groups, respectively, 4 weeks after moderate hypoxia treatment.', 'Analysis of global strain (F) and rate (G) together with apical longitudinal strain (H) and rate (I) showed significant deterioration of systolic function in UCP2KO animals compared with WT animals 4 weeks after moderate hypoxia treatment.', 'UCP2 ablation decreases cardiac function in adult mice under hypoxia.'] | Figure 4 UCP2 ablation decreases cardiac function in adult mice under hypoxia. ( ) Schematic illustration of the experimental plan shows WT and UCP2KO mice were kept in hypoxic chamber with daily drop of oxygen by 1% until it reached 7%. Mice were kept in 7% hypoxia for an additional 2 weeks, followed by functional and histological assessments. ( ) Moderate hypoxia treatment increases protein expression of UCP2 in WT mice as measured by immunoblot ( = 3 animals/group). LV tracebased analysis of cardiac M-mode images showed decreased cardiac function with lower LV ejection fraction (LVEF) ( ) and fractional shortening (FS) ( ) in UCP2KO animals compared with WT mice 4 weeks after hypoxia. Speckle-trackingbased strain imaging. ( ) 3-dimensional regional wall velocity diagrams show contraction (orange/positive values) or relaxation (blue/negative values) of consecutive cardiac cycles 4 weeks after moderate hypoxia. Vector diagrams show the direction and magnitude of endocardial contraction at mid-systole 4 weeks after moderate hypoxia. ( ) Global longitudinal strain (GLS) ( ), global longitudinal strain rate (GLS rate) ( ), apical longitudinal strain (ALS) ( ), and apical longitudinal strain rate (ALS rate) ( ) in mice lacking UCP2 compared with their WT littermates under hypoxia ( = 1519 male animals and = 810 female animals per group). Baseline versus Hypoxia: * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001; WT versus UCP2KO: < 0.05, < 0.001, < 0.0001. Data from , , and were analyzed using Kruskal-Wallis test with Dunns correction for multiple comparisons. | yes |
PMC8652403 | Figure_3 | oa_package/1a/54/PMC8652403.tar.gz | [] | FIGURE 3 (A) Computed tomography (CT) image, with yellow and blue arrows indicating the right and left mainstem bronchi, respectively, during inspiration. (B) CT image, with yellow and blue arrows displaying the right and left mainstem bronchi, respectively, during forced expiration | yes |
PMC4924419 | Figure_4 | oa_package/4f/99/PMC4924419.tar.gz | ['If a firmer skin crease is necessary, the sutures should incorporate the subcutaneous aponeurotic/septal tissue [].', '(a and b) Upper lid blepharoplasty.'] | Figure 4 (a and b) Upper lid blepharoplasty. Pre- and post-operative images | yes |
PMC9601696 | Figure_3 | oa_package/61/12/PMC9601696.tar.gz | ['Immunohistochemical studies demonstrate positive staining of the PHM, for both laminin and collagen IV, (a) providing additional evidence that this structure is a distinct basement membrane with the laminocytes integrally associated on scanning electron microscopy (b).', 'c shows a single laminocyte captured by confocal microscopy [68].', 'Immunohistochemistry and electron microscopy of PHM and laminocytes.'] | Figure 3 Immunohistochemistry and electron microscopy of PHM and laminocytes. ( ) PHM confocal surface topography micrograph stained with collagen IV antibodies, demonstrating distinct creased appearance. ( ) High power scanning electron micrograph of the PHM and associated laminocytes. (* laminocyte). The distinct folded vitreal aspect of the PHM (solid arrow) can be seen as a separate structure enveloping the fibrillary gel of the vitreous body (dashed arrow). ( ) Confocal micrograph of single laminocyte reveals unique large cashew shaped nucleus. (Tissue stained with DAPI, x100.8 magnification). | yes |
PMC10705149 | Figure_12 | oa_package/d9/f7/PMC10705149.tar.gz | [] | Figure 12 Left 5 cranial 15 ventral right caudodorsal oblique radiographic projection showing the right first and second ribs, obtained in a 3-year-old warmblood mare (the same horse as in ). The head of the horse is to the left. | yes |
PMC10635149 | Figure_3 | oa_package/fe/85/PMC10635149.tar.gz | ['At 24 hours post-infection we observed decreased expression of Nos2 and Tnfa in the colon of Chop / mice suggesting that CHOP has a role in the induction of inflammation (A and B).', 'At the later time points, Nos2 and Tnfa expression remained decreased and Il1b, Kc, and Il23 were also reduced ().', '565559v1-f0002" position="float"/>.'] | Figure 3. mice have reduced cytokine expression. RNA from the colon of infected and uninfected and littermate control mice was extracted and analyzed by qRT-PCR to determine the levels of (A), (B), (C), (D), and (E) at 24h, 48h and 72h post infection. Data shown as mean SEM with 58 mice per group. Unpaired t tests. p value *<0.05, **<0.01, ***<0.001 and ****<0.0001, ns (not significant) using GraphPad PRISM. | yes |
PMC5157054 | Figure_1 | oa_package/11/98/PMC5157054.tar.gz | [' 1a).', 'SB239063 and MW181 reduce inflammation-induced hyperphosphorylation of tau, p38 MAPK, and ATF2 activation in primary neurons.', '001; one-way ANOVA with Tukey s multiple comparison test)\nPrimary microglial cultureMicroglial cultures were prepared from postnatal day 3 (P3) pups from Cx3cr1\n / mice litters [42] as described previously [43].', ' 1a) in 2% FBS/DMEM to ensure adherence.', ' 1a c).', ' 1d, e).', ' 1d, e).', ' 1d, e).', ' 1d, e).', ' 1f, h).', ' 1j, k).', ' 1g, i).', ' 1g, i).', ' 1j, k).', ' 1 and 3).'] | Fig. 1 SB239063 and MW181 reduce inflammation-induced hyperphosphorylation of tau, p38 MAPK, and ATF2 activation in primary neurons. Schematic showing 30-min pretreatment of 21 DIV primary cortical neurons with MW181 (2M), SB239063 (100M), or vehicle (Veh) followed by treatment with 25% microglial conditioned media (CM) for 90min prior to biochemical analysis of neuronal lysates. , Structural formulae of SB239063 (adapted from [ ]) and MW181 (adapted from [ ]). , microglial CM significantly induced tau phosphorylation on AT8 and AT180 sites. Pretreatment of neurons with SB239063 or MW181 significantly reduced CM-induced tau phosphorylation on AT8 and AT180 sites. Quantifications are shown in ( =3 independent cultures, meanSEM of integrated density value (IDV) ratios as labeled. * <0.05; *** <0.001; one-way ANOVA with Tukeys multiple comparison test). MW181 ( ) or SB239063 ( ) neuronal treatment showing reduction in the activated p-p38 MAPK (pT180/pY182) and AT8 site tau phosphorylation with a maximum reduction at 60 and 90min time points (for MW181), and at 20 and 60min time points (for SB239063) post CM treatment, which was considered as 0min. Quantifications are shown in (for MW181) and (for SB239063). =3 independent cultures, meanSEM of IDV ratios as labeled. , Elevated levels of phosphorylated ATF2 (pT71) at 90min after microglial CM treatment. The pATF2 level was reduced by 30-min pretreatment with SB239063 and MW181. Quantifications are shown in ( =3 independent cultures, meanSEM of IDV ratios of pATF2/GAPDH; ** <0.01; *** <0.001; one-way ANOVA with Tukeys multiple comparison test) | yes |
PMC10395180 | Figure_3 | oa_package/14/e1/PMC10395180.tar.gz | ['App Store Connect (also known as iTunes Connect) is an Apple web-based tool to manage apps and users, providing reports with statistics to monitor the performance of apps ().', 'App Store Connects includes:\nMy AppsApp AnalyticsSales and TrendsPayments and Financial ReportsUsers and AccessAgreements, Tax, and Banking.', '1177_23821205231192341-fig3" position="float"/>App Analytics provides a full report on the performance of apps and usage for selected time frames.'] | Figure 3. Apple Store Connect. | yes |
PMC11254316 | Figure_1 | oa_package/93/15/PMC11254316.tar.gz | ['Nervus facialis.'] | Figure 1 Nervus facialis. The presented figure is original artwork. | yes |
PMC4505608 | Figure_7 | oa_package/90/bb/PMC4505608.tar.gz | ['This was followed by surface and intracellular staining of defined markers for NK cells, CD8 T cells, and granzyme B ().', 'In the pLN, high levels of granzyme B were detected predominantly in the NK cells and not in CD8 T cells at 3 dpi (A).', 'Similarly, higher levels of granzyme B were also detected in the NK cells of the joint footpad at 3 dpi (C).', 'Differential regulation of granzyme B expressing CD8 T cells was observed between LR2006 OPY1 and CNR20235 (C and D).', 'Interestingly, depletion of NK cells significantly reduced joint footpad swelling in LR2006 OPY1 and not in CNR20235 infection at 3 dpi (E).'] | FIG 7 Early acute NK activation in LR2006 OPY1 mediates joint pathology. Three-week-old WT C57BL/6 female mice were infected with LR2006 OPY1 or CNR20235 at 10 TCID ( = minimum 5 mice per group) by joint footpad inoculation. (A and C) Mean fluorescence intensity (MFI) representing granzyme B expression in CD8 and NK cells in pLNs (A) and footpads (C) in CHIKV infection (two-tailed Mann-Whitney U test). *, = 0.0476 (pLN, 3 dpi, CD8); *, = 0.0317 (pLN, 3 dpi, NK); *, = 0.0476 (footpad, 6 dpi, CD8). (B and D) Absolute counts of granzyme B CD8 and NK cells in pLNs (B) and footpads (D) in CHIKV infection (two-tailed Mann-Whitney U test). *, = 0.0317 (pLN, 3 dpi, CD8); *, = 0.0317 (pLN, 3 dpi, NK); *, = 0.0317 (footpad, 6 dpi, CD8); *, = 0.0317 (footpad, 6 dpi, NK). NK cells were depleted from mice using -asialo-GM1 antibody, and equal amounts of rabbit serum were given for controls. (E) Disease scores at 3 and 6 dpi in CHIKV infection (two-tailed Mann-Whitney U test). **, = 0.0079 (nsP1, day 6); ***, < 0.0001 (disease score, day 6). | yes |
PMC1851853 | Figure_1 | oa_package/da/e7/PMC1851853.tar.gz | ['Signs of clinical CNS disease were monitored daily and scored using standards as described in .', 'However, clinical signs of CNS disease occurred earlier and were stronger in the THMOG mice compared with Bl6 controls (, top).', 'The three viral doses also resulted in an increased death rate in THMOG mice in comparison with controls (, bottom).', 'The outcome of MHV A59 acute infection of the CNS is exacerbated in THMOG knock-in mice.'] | Figure 1 The outcome of MHV A59 acute infection of the CNS is exacerbated in TH knock-in mice. Mice were observed and given a numerical score (top panels) for behavioral signs of encephalitis (0, no detectable sign of disease; 1, ruffled fur; 2, slightly hunched back and ruffled fur; 3, very hunched back and lethargy; and 4, death) daily during the acute infection phase in mice infected with increasing doses of virus (left to right). Data represent the average and standard errors for each group. The bottom panels show the survival of the animals in the same experiments. * < 0.05; ** < 0.01; *** < 0.001. | yes |
PMC10749253 | Figure_7 | oa_package/16/9e/PMC10749253.tar.gz | [' shows the examples of predicted masks generated using the proposed model compared with other segmentation methods.', 'Illustration of proposed model s segmentation results against other methods for CD3, CD4, and CD8.', 'Considering the segmentation performance of the proposed model for these three biomarkers, we established a satisfactory level of confidence to extend our analysis to an independent subset consisting of 141 patients.'] | Fig. 6 Illustration of proposed models segmentation results for CD3, CD4, and CD8. Exam- ples of each T-cell type were chosen, showing manual annotation and model output. | yes |
PMC10322670 | Figure_4 | oa_package/47/95/PMC10322670.tar.gz | [] | Figure 4 Biopsy of the left ankle (A) Dense lymphocytic infiltrate in the reticular dermis and subcutaneous tissue with prominent periadnexal and peri-vascular distribution (H&E, x 20). Immunohistochemistry shows the neoplastic cells diffusely positive for CD3 (B; x 200) and CD7 (C; x 200). (D) Epstein-Barr encoding region (EBER) in-situ hybridization is positive (EBER ISH, x 200) | yes |
PMC8996041 | Figure_2 | oa_package/f6/8d/PMC8996041.tar.gz | ['Non-enhancing T2 signal white matter changes at the anterior left frontal lobe especially along frontal horn lateral ventricle, as well as a non-specific tiny focus of superior left frontal lobe, unchanged between MRIs.'] | Figure 2 Non-enhancing T2 signal white matter changes at the anterior left frontal lobe especially along frontal horn lateral ventricle, as well as a non-specific tiny focus of superior left frontal lobe, unchanged between MRIs. | yes |
PMC4488051 | Figure_4 | oa_package/54/21/PMC4488051.tar.gz | [' 4a).', ' 4a).', ' 4b), indicating that TNF synthesis by neurons is not required to control infection.', ' 4c, e), indicating that TNF is required to control early dissemination of infection.', ' 4c, e) or chronic infection (', ' 4d, f) suggested a redundancy of neuron-derived TNF to contain cerebral spread of infection.', 'TNF is required to control intracerebral M.', '01Cerebral pathology is regulated by TNF but neuron-derived TNF is not required.'] | Fig. 4 TNF is required to control intracerebral replication but neuron-derived TNF is redundant. TNF ( ), NsTNF ( ) and TNF ( ) mice ( = 56/group) were infected with at a dose of 1 10 1 10 CFUs/brain. Bacterial burdens in , brains; , spleens; and , lungs at 1, 2, 3 ( ; , , , respectively) and 15weeks ( ; , , respectively) post-infection were determined. Data (mean SEM of the CFUs of 56 mice per time point) are representative of three similar experiments. * 0.05; ** 0.01 | yes |
PMC7744471 | Figure_2 | oa_package/2f/46/PMC7744471.tar.gz | ['s 2A shows a hypertrophic kidney from a DN model group mouse compared with one from the NC group.', 'In contrast to the NC group, HE staining showed that glomerular volume was enlarged in DN group mice, and the number of cells was increased, mesangial matrix proliferated, cells around the glomerulus were disordered, and there was edema (s 2B).', 'PAS staining showed glomerular hypertrophy, basement membrane thickening, and increased glycogen in the DN model group (s 2C).', 'This analysis revealed podocyte fusion and glomerular basement membrane thickening in the model group, and these pathological changes were ameliorated by treatment with BSHX decoction and metformin (s 2D,E).', 'FIGURE 2BSHX alleviates renal histopathology and ultrastructural pathology of the kidneys.'] | FIGURE 2 BSHX alleviates renal histopathology and ultrastructural pathology of the kidneys. Preliminary observation of the appearance and size of kidneys. Hematoxylin and eosin (HE) staining of the kidney (400). Periodic acid Schiff (PAS) staining of the kidney (400). Transmission electron microscopy of the kidney (scale bar, 2m). Transmission electron microscopy of the kidney (scale bar, 500nm). = 5 mice per group. | yes |
PMC9477559 | Figure_2 | oa_package/02/b5/PMC9477559.tar.gz | ['(A) and (B) images showing different MRI parasagittal sections of the spine with myelography representing abnormal signal intensity with anterior wedge compression in D11 and D12.'] | Figure 2 (A) and (B) images showing different MRI parasagittal sections of the spine with myelography representing abnormal signal intensity with anterior wedge compression in D11 and D12. | yes |
PMC11070229 | Figure_3 | oa_package/e8/5b/PMC11070229.tar.gz | ['H E 100 , section showing ovoid to fusiform spindle cells with indistinct cell borders arranged haphazardly or in short, ill-defined fascicles, dilated, branching, hyalinized staghorn-like (hemangiopericytoma-like) vasculature.', '3DiscussionVarious studies have highlighted the challenges in accurately diagnosing and managing AVMs.'] | Fig. 3 H&E 100, section showing ovoid to fusiform spindle cells with indistinct cell borders arranged haphazardly or in short, ill-defined fascicles, dilated, branching, hyalinized staghorn-like (hemangiopericytoma-like) vasculature. | yes |
PMC3868760 | Figure_4 | oa_package/d3/55/PMC3868760.tar.gz | ['Interestingly, these differences in expression of IFN pathway genes such as Irf7, Ifit2, Isg15, and Stat1 were apparent (A, panel i), despite there being no significant alterations in the temporal expression patterns of either IFN or IFN (', ' A number of the other overexpressed type I IFN pathway genes showing the most specific elevation in D6-deficient, compared with WT, mice are shown in the heat map in B.', 'To confirm that the IFN pathway was up-regulated in the skin of D6-deficient, compared with WT, mice, quantitative PCR was performed for Irf7, Ifit2, and CXCL9 using RNA derived from a separate skin inflammation study (C).'] | FIGURE 4. , , profile plots demonstrating differences in the levels of induction of type I interferon pathway genes , , , and in WT ( ) and KO ( ) inflamed mouse skins. , profile plots revealing the similarity in the induced expression levels of IFN- and IFN- in WT and KO skins over the course of the induction of inflammation. In both and , the data are expressed as normalized intensity values (log ; axis) over time (days; axis). *, < 0.05; **, < 0.01; ***, < 0.001; ****, < 0.0001. , heat map analyses of the differential expression of a select group of type I interferon pathway genes over the course of the study in WT and D6-deficient (KO) mice after TPA treatment. , no change; , down-regulated; , up-regulated. The time points are indicated along the of the heat map (for , indicates WT day 0, 1 indicates WT day 1, etc.). , confirmatory PCR demonstrating increased expression of type I interferon pathway genes in inflamed D6 KO compared with WT skins. , , , CXCL9. These PCR analyses were performed on skin samples isolated from an experiment separate from that used to generate the array data. The data are shown as absolute copy number of each gene compared with 10 copies of -actin. | yes |
PMC7676494 | Figure_3 | oa_package/ba/55/PMC7676494.tar.gz | ['(a) and (b) BF, DF and Alexa488 and Alexa647 Fluorescence (FL1 and FL2, respectively) typical images of the z-stack obtained with the oCelloscope.'] | Fig. 3 (a)and (b)BF, DF and Alexa488 and Alexa647 Fluorescence (FL1 and FL2, respectively) typical images of the z-stack obtained with the oCelloscope. The scale bar depicted in the right panel represents . (c)H&E-stained tissue section, and (d)cross section of the confocal microscope showing the same structures with CK8-18 positive luminal epithelial cells (green) and CK5 positive basal epithelial cells (red) in benign transition zone prostate glands. | yes |
PMC3202547 | Figure_2 | oa_package/45/b1/PMC3202547.tar.gz | ['Daily subconjunctival administration of 20 mIU of insulin for 4 consecutive days to rats with 4 weeks of STZ-induced diabetes did not reduce the hyperglycemia (A).', 'Similarly, no differences in serum insulin levels were detected between the treated and untreated diabetic rats, demonstrating that subconjunctival administration of low doses of insulin had no effect on the systemic hypoinsulinemic condition (B).', 'Phloridzin administration significantly reduced mean serum glucose levels of diabetic rats from 531 mg/dL and maintained them at normal levels (206 mg/dL) for at least 6 h post-injection at day 2 (A).', 'Phloridzin had no effect on serum insulin levels confirming that the effect of phloridzin on serum glucose levels is independent of plasma insulin (B).', 'Specificity of the treatments was also observed when rats with longer duration of diabetes (12 weeks) were treated (C and 2D).', 'In correlation with the systemic results, the mean vitreous glucose concentrations were reduced from means of 448 to 254 mg/dL by phloridzin treatment, but were unaffected by local insulin administration (E and 2F), and retinal insulin levels did not increase in phloridzin treated rats (G).', 'In contrast, subconjunctival injection of insulin in diabetic rats lead to significantly increased retinal insulin levels (G).', 'Neither local insulin administration nor systemic phloridzin treatment significantly affected the body weights or food consumption of rats during the 4 days of treatment (H).', 'g002Distinct physiological effects of subconjunctivally delivered insulin and systemic phloridzin treatment.', 'Likewise, we demonstrated that twice daily subcutaneous administration of the SGLT1 and SGLT2 glucose transporter inhibitor phloridzin, restored normal glycemia without affecting systemic or ocular levels of insulin ().'] | 10.1371/journal.pone.0026498.g002 | yes |
PMC8687144 | Figure_3 | oa_package/80/0b/PMC8687144.tar.gz | ['The mitral valve showed a large cleft in the anterior leaflet segment A1/A2, with A1 partially connected to the ventricular wall ().', 'The tricuspid valve also had several mild nodular thickenings at its free margin ().', '(A) Left ventricular free wall and posterior mitral valve showing enlarged middle scallop (arrowhead).'] | Figure 3 Left ventricular free wall and posterior mitral valve showing enlarged middle scallop (arrowhead). Septum as viewed from the left ventricle showing ASD and cyst. The anterior mitral leaflet is exhibiting diffuse thickening (*), and A1/A2 clefting (arrow). Right ventricular free wall and tricuspid valve with small nodular thickenings at the free margin (arrows). | yes |
PMC4899632 | Figure_4 | oa_package/82/10/PMC4899632.tar.gz | ['Striatal engraftment of the R6/2 mice by CD44-sorted hGPCs was robust (a,b), and achieved densities of 15,000 human cells per mm3 by 16 weeks of age (', 'The human CD44-sorted glia integrated as both astrocytes (d and Table 2) and as persistent GPCs (', '4e,f and Table 2), but not as neurons (g).', 'Importantly, the integrated human cells did not manifest detectable HTT aggregates; the staining patterns of HTT and human nuclear antigen were always entirely non-overlapping (h).', '00001) of age (Supplementary ).', 'org/1999/xlink" xlink:href="ncomms11758-f3"/>CD44-sorted hGPCs colonized and replaced endogenous glia within the R6/2 rag1 / striatum.'] | Figure 4 CD44-sorted hGPCs colonized and replaced endogenous glia within the R6/2 rag1 striatum. Striatal engraftment of the R6/2 mice by CD44-sorted hGPCs was robust and dense. ( , ) Fetal derived cells expanded to colonize the striata and ventral forebrain of engrafted mice by 20 weeks. ( ) Donor-derived cells in the striata of transplanted mice increased as a function of time (meanss.e.m.). ( ) By 20 weeks after neonatal graft, the donor hGPCs (human nuclear antigen, red) integrated as astrocytes ( ; GFAP, green) or persisted as GPCs ( , ; PDGFR and olig2, green), but did not give rise to neurons; no overlap was ever seen of hNA and NeuN expression ( ; NeuN, green). ( ) Resident human glia did not manifest detectable nuclear Htt aggregates, as assessed by EM48 immunostaining; the staining patterns of host Htt and donor human nuclear antigen were always entirely non-overlapping ( ). Scale bars, 1mm ( , ); 25m ( ). | yes |
PMC7641380 | Figure_6 | oa_package/fd/32/PMC7641380.tar.gz | ['g006Case 19: MRI of a 3 year old male patient with unspecified form.'] | 10.1371/journal.pone.0241436.g006 | yes |
PMC5559642 | Figure_1 | oa_package/b0/a2/PMC5559642.tar.gz | [] | FIGURE 1 Photomicrograph of control and infected catfish spleen. Photomicrograph of control spleen showed normal architecture with ellipsoids (E) beside mixed red and white pulp (P). Infected spleen showed mild splenitis with an accumulation of proteinaceous substance around ellipsoid arterioles (arrow) at 5 HPC, 100. Moderate splenitis with numerous macrophages and lymphocytes aggregated around ellipsoids (arrow) in addition to hemosiderosis (H) at 24 HPC, 400. Severe splenitis with diffuse fibrinoid necrosis of ellipsoidal sheath (thick arrow) and aggregation of inflammatory cells (thin arrow) at 48 HPC, 400, H&E. | yes |
PMC4186498 | Figure_2 | oa_package/6b/98/PMC4186498.tar.gz | ['Abdominal images revealed a large concentric mass involving the ascending colon that extended ~7 cm in length with marked narrowing of the colon, without causing complete obstruction ().', 'Computed tomography scan of the abdomen showing (A) the proximal colon mass and (B) the large peri-aortic lymph nodes.'] | Figure 2 Computed tomography scan of the abdomen showing (A) the proximal colon mass and (B) the large peri-aortic lymph nodes. | yes |
PMC5731869 | Figure_6 | oa_package/3b/a5/PMC5731869.tar.gz | ['There were no significant differences in overall survival between RC low and high grade groups (Supplementary A - 6E) for eIF2S1, eIF3A, eIF3B, eIF3C, eIF3D and eIF3H.', 'In vitro characterization of eIF5 knockdown effect in HCT116 cells(A) Protein expression of eIF5-siRNA knockdown after 24h, 48h and 72h compared to SC.'] | Figure 6 characterization of eIF5 knockdown effect in HCT116 cells Protein expression of eIF5-siRNA knockdown after 24h, 48h and 72h compared to SC. mRNA expression level of in HCT116 cells compared to SC. Cell viability in HCT116 cells transfected with eIF5 siRNA after 24h, 48h and 72h. Graphs show apoptosis rate after eIF5-siRNA knockdown compared to the SC after 24h, 48h and 72h. Three independent experiments were carried out. Bars represent mean SEM. p < 0.05, p < 0.01, p < 0.001. Statistical analysis: 2-way ANOVA with Bonferroni posttest. | yes |
PMC10033986 | Figure_3 | oa_package/6a/33/PMC10033986.tar.gz | ['At the end of the procedures performed via a transradial approach, hemostasis was achieved by applying the Vasc Band Hemostat (Teleflex) for 1 hour (Fig 3).', 'Fig 3Hemostasis with a Vasc Band Hemostat after the transradial approach.'] | Fig3 Hemostasis with a Vasc Band Hemostat after the transradial approach. Inflation of the Vasc Band Hemostat applies direct compression to the radial artery distal to the arteriovenous anastomosis. | yes |
PMC11710901 | Figure_2 | oa_package/b1/a7/PMC11710901.tar.gz | [] | FIGURE 2 (A) Histopathologic examination of the lesion demonstrated sheets and lobules of cells separated by fibrous septa (H&E, original magnification 8). (B) Dense lobules and sheets of atypical small round blue cells were present (H&E, original magnification 80). (C) A higher power view of the sheets and lobules of atypical round blue cells with significant cytologic atypia and mitotic figures (H&E, original magnification 400). | yes |
PMC11381857 | Figure_2 | oa_package/39/24/PMC11381857.tar.gz | ['(A and B) CT image in soft (A) and bone (B) window (C).', "(A) MRI axial T1 fatsat weighted image showing a small mass in the surgical neck of the right humerous bone, (B) MRI axial T1w fatsat image with in contrast showing the periosteal reaction with no enchantment of the mass, (C) DWI sequence, and (D) ADC map didn't reveal any restriction of water molecules in mass."] | Fig. 2 A Spect showing increased blood pool and increased 99mTc-Methylene diphosphonate. | yes |
PMC11395180 | Figure_1 | oa_package/d5/e8/PMC11395180.tar.gz | [' 1).', '\nHistological findings in CIG patients.', 'From left to right: Patient 2 (A, E), Patient 5 (B, F), Patient 14 (C, G) and Patient 7 (D, H)\nmNGS sequencing and analysisThe entire pathogen detection pipeline and sequencing process was completed in the Cellular Molecular Diagnostics Center of Sun Yat-sen memorial hospital, Sun Yat-sen University, Guangzhou, China.'] | Fig. 1 Histological findings in CIG patients. , H&E, Bar =1.25mm, , H,&E, Bar =50m, and H, H&E, Bar =200m.G, H&E, Bar =100m. From left to right: Patient 2 ( ), Patient 5 ( ), Patient 14 ( ) and Patient 7 ( ) | yes |
PMC9372862 | Figure_1 | oa_package/d7/be/PMC9372862.tar.gz | ['\nTimeline of this case report.'] | Figure 1 CT: Computed tomography; MRI: Magnetic resonance imaging; WHO: World Health Organization. | yes |
PMC10771002 | Figure_1 | oa_package/24/dd/PMC10771002.tar.gz | [' 1).', '\n(a, c, e, g, and i): Coronal view of the teeth, (b, d, f, h, and j): Sagittal view of the teeth.', '(a, b): Upper left first molar with missed MB2 canal, PL, and a PFM crown; (c, d): Upper left first molar with a underfilled palatal canal; (e, f): Upper right first molar with a screw in the palatal canal, PL, and composite restoration; (g, h): Lower right first molar with separated instrument in the distal canal and PL; (i, j); Lower left first molar with furcal lesion (excluded from the study)\nData on the four dependent variables were recorded: presence of PL, PL volume, presence of CBD, and BPBH.'] | Fig. 1 ( , and ): Coronal view of the teeth, ( , and ): Sagittal view of the teeth. ( ): Upper left first molar with missed MB2 canal, PL, and a PFM crown; ( ): Upper left first molar with a underfilled palatal canal; ( ): Upper right first molar with a screw in the palatal canal, PL, and composite restoration; ( ): Lower right first molar with separated instrument in the distal canal and PL; ( ); Lower left first molar with furcal lesion (excluded from the study) | yes |
PMC6753395 | Figure_1 | oa_package/c0/70/PMC6753395.tar.gz | ['Chest X-ray and computed tomography (case 1).'] | Figure 1 Chest X-ray and computed tomography (case 1). A: Chest X-ray demonstrating the bilateral patchy infiltrates at the time of admission; B: Computed tomography scans of chest with coronal section demonstrating bilateral patchy infiltrates at the time of admission; C: X-ray chest demonstrating worsening of the bilateral infiltrates after intubation and initiation of anti tuberculosis medications. | yes |
PMC11365134 | Figure_2 | oa_package/f4/4f/PMC11365134.tar.gz | [' 2A).', ' 2B and C).', ' 2D and E).', ' 2F and H).', ' 2G and H).', ' 2I).', ' 2J).', '\nKnockout of HK2 alleviates kidney IRI in mice.', '01\nAST-120 attenuates renal injury in I/R mice by regulating HK2Subsequently, we analyzed the role of AST-120 in vivo.'] | Fig. 2 Knockout of HK2 alleviates kidney IRI in mice. ( ) Protein levels of HK2 were examined using western blotting in WT or HK2-KO mice in the sham and I/R groups. ( ) SCr and ( ) BUN levels in the serum of mice were measured using corresponding commercial kits. ( ) KIM-1 and ( ) NGAL expression in the renal tissues of mice were measured using qPCR. ( ) Kidney injury score according to the H&E staining. ( ) The percentage of TUNEL positive cells was quantified. (H) Representative images of H&E staining (scale bar: 200m; red arrows: injured tissue) and TUNEL assays (scale bar: 50m). ( ) Lactate levels were measured using a corresponding kit. (J) Platelets were isolated from the blood of mice, and ECAR was analyzed using a Seahorse assay. ** <0.01 | yes |
PMC3334941 | Figure_7 | oa_package/2f/bb/PMC3334941.tar.gz | ['However, these artefacts can be detected even in averaged summary scans by looking at the outer nuclear layer (ONL) to the ONL reflectivity () <xref rid="pone.', 'g007Beam placement: the laser beam is not placed centrally.'] | 10.1371/journal.pone.0034823.g007 | yes |
PMC8165089 | Figure_3 | oa_package/73/d7/PMC8165089.tar.gz | ['Following the start of the COVID-19 pandemic, thousands of children and young adults with no prior history of acral skin changes began to develop asymptomatic, painful, and/or pruritic lesions with striking resemblance to pernio: erythematous, purpuric papules, and macules affecting the toes, feet, fingers, and hands (\nmorphologies are discussed in further detail in Ritesh Agnihothri and Lindy P.', '56\n'] | Fig.3 Pernio-like lesions in children. (A) A child with purpuric papules on the 1st, 2nd, 4th, and 5th right digits and 2nd proximal left digit and (B) Digits on the same child appearing with increased erythema. (C) Right toes of a child appearing with pink and dusky papules and plaques, also involving (D) the childs left digits. | yes |
PMC6827223 | Figure_3 | oa_package/8e/ad/PMC6827223.tar.gz | [' 3).', 'a Optic nerves of mice 120 days after MOG or MBP immunization stained by H E and CD3, Iba1, or MBP antibodies.', ', not significant, by ANOVA with Dunnett s post hoc test compared to sham-treated miceIncreased T cell infiltration, microglial activation, and demyelination of the optic nerve were observed 120 days after immunization of WT mice with MOG35 55 peptide.', ' 3).'] | Fig. 3 Optic nerves of mice 120days after MOG or MBP immunization stained by H&E and CD3, Iba1, or MBP antibodies. Optic nerves were compared by an established infiltration score according to H&E staining ( ) (Shindler et al. [ ]), MBP score for the myelin status ( ), Iba1 fluorescence intensity measurement for microglia activation ( ), and CD3+ cell infiltration ( ). The bar graphs represent the pooled meanstandard deviation of at least two separate EAE experiments each with at least four animals per group; one optic nerve per mouse was included, * <0.05; ** <0.01; *** <0.001; n.s., not significant, by ANOVA with Dunnetts post hoc test compared to sham-treated mice | yes |
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