id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.kfactie | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
48,718 | Untargeted Top-down Proteomics by LC-MS/MS on Lumos | 1 | dx.doi.org/10.17504/protocols.io.bttnnnme | https://www.protocols.io/view/untargeted-top-down-proteomics-by-lc-ms-ms-on-lumo-bttnnnme | Bryon Drown, Jeannie Camarillo, Neil Kelleher | TITLE: Untargeted Top-down Proteomics by LC-MS/MS on Lumos
AUTHORS: Bryon Drown, Jeannie Camarillo, Neil Kelleher
[DESCRIPTION]
Describes the LC-MS/MS data acquisition procedure for top-down proteomics samples using the Thermo Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer
[STEPS]
1. Samples were analy... | ["Samples were analyzed on a Thermo Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer in line with a Dionex Ultimate 3000 RSLCnano system", "Samples (6 µL ) were injected via the autosampler and loaded onto a self-packed trap column (150 μm i.d. x 2 cm length packed with PLRP-S 5-μm particles 1,000-Å pore size... |
75,985 | Production of Rhizoctonia solani inoculum for sugar beet disease trials | 1 | dx.doi.org/10.17504/protocols.io.dm6gpjo78gzp/v1 | https://www.protocols.io/view/production-of-rhizoctonia-solani-inoculum-for-suga-cnfrvbm6 | bradley.sowder, Travis Vagher, nicholas.metz, Amy Nielson, kevin.dorn | TITLE: Production of Rhizoctonia solani inoculum for sugar beet disease trials
AUTHORS: bradley.sowder, Travis Vagher, nicholas.metz, Amy Nielson, kevin.dorn
[DESCRIPTION]
Rhizoctonia solani is an economically important plant pathogen resulting in substantial losses of crop production within the United States and wor... | ["[Preparation of culture for long term storage] Obtain desired isolate from either fungal isolation techniques or material transfer from a third party resource.", "[Preparation of culture for long term storage] Prepare petri plates containing 25ml of potato dextrose agar and place sterilized medium to be colonized for... |
81,443 | a protocol for the detection mitochondrial heteroplasmy in a non model organism from whole genome sequencing data | 5 | null | https://www.protocols.io/view/a-protocol-for-the-detection-mitochondrial-heterop-ctsbwnan | Ramawatar Nagar | TITLE: a protocol for the detection mitochondrial heteroplasmy in a non model organism from whole genome sequencing data
AUTHORS: Ramawatar Nagar
[DESCRIPTION]
In the great majority of sexual eukaryotic organisms, mitochondrial inheritance is uniparental (usually maternal) which is tightly regulated to maintain homopl... | ["Mitochondrial genome assembly: Assemble the mitochondrial genome from the sequencing data using a reference genome or de novo assembly tools like MitoZ or MITObim. For non-model organisms, a reference genome may not be available. In such cases, you can use a related species as a reference or perform de novo assembly ... |
null | null | null | dx.doi.org/10.17504/protocols.io.e2fbgbn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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89,175 | QC Metrics for KPMP Data Collection by VU TIS | 1 | dx.doi.org/10.17504/protocols.io.14egn3wqyl5d/v1 | https://www.protocols.io/view/qc-metrics-for-kpmp-data-collection-by-vu-tis-c3bxyipn | Katerina V Djambazova, Lukasz Migas, Jamie Allen, Madeline E. Colley, Martin Dufrense, Allison Esselman, Léonore Tideman, Ellie Pringy, Angela R.S. Kruse, Melissa Farrow, Raf Van De Plas, Jeff Spraggins | TITLE: QC Metrics for KPMP Data Collection by VU TIS
AUTHORS: Katerina V Djambazova, Lukasz Migas, Jamie Allen, Madeline E. Colley, Martin Dufrense, Allison Esselman, Léonore Tideman, Ellie Pringy, Angela R.S. Kruse, Melissa Farrow, Raf Van De Plas, Jeff Spraggins
[DESCRIPTION]
This protocol describes the Go/No-Go cri... | ["[Biopsy Screening & Go/No-Go Criteria] To accept KPMP samples for analysis, the following sample processing Go/No-Go criteria in Table 1 must be met:\n\nTable 1. Sample Processing Go/No-Go Criteria\n AB1Go/No-Go Criteria Reasoning 2Biopsy must be LN2 core Other storage conditions can induce analyte suppressi... |
21,423 | Isolation of Nuelci from Adult_Human_Brain_Tissue | null | dx.doi.org/10.17504/protocols.io.y6pfzdn | null | LeinU01 BRAIN Grant | TITLE: Isolation of Nuelci from Adult_Human_Brain_Tissue
AUTHORS: LeinU01 BRAIN Grant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis. </div></div>
[STEPS] | [] |
67,669 | Annealing Oligonucleotides | 4 | null | https://www.protocols.io/view/annealing-oligonucleotides-cebvtan6 | Brian P P Teague | TITLE: Annealing Oligonucleotides
AUTHORS: Brian P P Teague
[DESCRIPTION]
While most biologically-synthesized DNA molecules are double-stranded, chemically-synthesized DNA molecules are single-stranded. In order to use them in a biological system, we need to make them double-stranded by "sticking" them to their comple... | ["[Resuspending oligos] If the oligonucleotides have already been resuspended, skip to step 5.\n\nLook carefully at the label of the oligo that you're resuspending. Find the line that looks like \"14.5 OD = 26.1 nmol = 0.46 mg\". (This is an example; the numbers on your tube will be different.) These are three differen... |
107,543 | Purification of Human K560-GFP motor (without B+R) | 4 | dx.doi.org/10.17504/protocols.io.4r3l2qr1xl1y/v1 | https://www.protocols.io/view/purification-of-human-k560-gfp-motor-without-b-r-dk9x4z7n | Andrea M. Dickey, Katherine Surridge | TITLE: Purification of Human K560-GFP motor (without B+R)
AUTHORS: Andrea M. Dickey, Katherine Surridge
[DESCRIPTION]
K560-GFP purification protocol from the Reck-Peterson Lab based on protocol from Nicholas et al. 2014. Edited for protocols.io by Andrea Dickey and Mariusz Matyszewski in 2022 and modified in 2024 for ... | ["[Expression] pET17b-Kif5b(1-560)-GFP-His should be transformed into BL21(DE3)RIPL cells.", "[Expression] Make enough LB for at least 2 L of culture.", "[Expression] Grow an overnight starter culture.", "[Expression] Transfer starter culture into LB. Make sure to add proper antibiotics\nAllow it to grow at until OD6... |
29,152 | Reporter titrations for transfection of Micromonas commoda CCMP2709 | null | dx.doi.org/10.17504/protocols.io.8p8hvrw | null | Lisa Sudek, Alexandra Worden, Manny Ares | TITLE: Reporter titrations for transfection of Micromonas commoda CCMP2709
AUTHORS: Lisa Sudek, Alexandra Worden, Manny Ares
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the method used to test various amounts (0, 0.3, 3, 10, 20 and 30 µg) of reporter (</span><span s... | ["[Plasmid Preparation]\nThe Carrier DNA stock (pUC19) is purchased at a concentration of 20 µg µl-1 in 10 mM TrisHCl pH8, aliquoted in 100 µl volumes and stored at -20 °C.", "[Plasmid Preparation]\nThe reporter plasmid stock (in this case of plasmid H3proMpo-LUC) is prepared by transforming the purchased plasmid into ... |
64,611 | Intraganglionic injection of AAV into thoracic dorsal root ganglia in mice | 1 | dx.doi.org/10.17504/protocols.io.n92ldz6z9v5b/v1 | https://www.protocols.io/view/intraganglionic-injection-of-aav-into-thoracic-dor-cbcbsisn | Seol-Hee Kim, patil, Thomas Taylor-Clark | TITLE: Intraganglionic injection of AAV into thoracic dorsal root ganglia in mice
AUTHORS: Seol-Hee Kim, patil, Thomas Taylor-Clark
[DESCRIPTION]
We have developed a novel method of injecting AAV vectors directly into the sensory ganglia, vagal and dorsal root ganglia, of mice. This technique provides for the first ti... | ["Mice were anesthetized with a mixture of ketamine (50 mg/kg) and dexmedetomide (0.5 mg/kg) via intraperitoneal injection.", "Approximately 4 cm of incision was made over a shaved portion of the mouse back region, just around the neck area.", "The skin flaps were opened, and the neck muscles were identified. The C7 an... |
null | null | null | dx.doi.org/10.17504/protocols.io.s2gegbw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Mouse antibody types</strong></p>
<p>It is same as human beings that there are five antibody isotypes, including IgA, IgD, IgG and IgM, from mice. Different isotype has different heavy chain. IgG and IgM are produced by native B cells, which will produce one of IgG or... | [] |
67,104 | How to effectively control vertical collusion in bidding for government investment projects-Based on fsQCA method | 2 | dx.doi.org/10.17504/protocols.io.cdr8s59w | https://www.protocols.io/view/how-to-effectively-control-vertical-collusion-in-b-cdr8s59w | machongsen, Yun Chen, Liang Ou, Wenxi Zhu | TITLE: How to effectively control vertical collusion in bidding for government investment projects-Based on fsQCA method
AUTHORS: machongsen, Yun Chen, Liang Ou, Wenxi Zhu
[DESCRIPTION]
The impact of collusion during the bidding processes of Chinese government investment projects is a major concern in academic and pol... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.chrt55 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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53,250 | NEBNext® Varskip Short ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658 Express Protocol with One Clean-up Step | 4 | dx.doi.org/10.17504/protocols.io.bx9apr2e | https://www.protocols.io/view/nebnext-varskip-short-artic-sars-cov-2-fs-library-bx9apr2e | New England Biolabs | TITLE: NEBNext® Varskip Short ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658 Express Protocol with One Clean-up Step
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®)... | ["[cDNA Synthesis]\nGently mix and spin down the LunaScript RT SuperMix reagent. Prepare the cDNA synthesis reaction as described below:COMPONENTVOLUMERNA Sample8 µl\n(lilac) LunaScript RT SuperMix2 µlTotal Volume10 µlFor no template controls, mix the following components: COMPONENTVOLUME(white) Nuclease-free Water8 µl... |
86,524 | Electrophysiological recording from a chronic chamber-implanted non-human primate | 1 | dx.doi.org/10.17504/protocols.io.bp2l6xx91lqe/v1 | https://www.protocols.io/view/electrophysiological-recording-from-a-chronic-cham-cyq4xvyw | Witold J Lipski, Daisuke Kase, Devin R Harsch, Robert S Turner | TITLE: Electrophysiological recording from a chronic chamber-implanted non-human primate
AUTHORS: Witold J Lipski, Daisuke Kase, Devin R Harsch, Robert S Turner
[DESCRIPTION]
This protocol describes the preparation and insertion of extracellular recording electrodes into intracranial targets of a non-human primate imp... | ["[Preparation before the day of recording] Choose the target area using resources such as an MRI or prior brain mapping.", "[Preparation before the day of recording] Calculate the distance from the micro-drive to the surface of the brain.", "[Preparation before the day of recording] Draw a mark on the dura piercing gu... |
33,385 | Bench top CUT&Tag | null | dx.doi.org/10.17504/protocols.io.bcuhiwt6 | null | Hatice Kaya-Okur, Steven Henikoff | TITLE: Bench top CUT&Tag
AUTHORS: Hatice Kaya-Okur, Steven Henikoff
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here we describe a bench top protocol for Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries f... | ["[Bind cells or nuclei to Concanavalin A-coated beads (30 min)]\nGently resuspend and withdraw enough of the ConA bead slurry such that there will be 10 μL for each final sample of up to 500,000 cells. The following is for 16 samples.", "[Bind cells or nuclei to Concanavalin A-coated beads (30 min)]\nTransfer 170 µL C... |
98,526 | Human CD34+ cell isolation from fetal liver, and fetal thymus preparation | 4 | dx.doi.org/10.17504/protocols.io.6qpvrdw3ogmk/v2 | https://www.protocols.io/view/human-cd34-cell-isolation-from-fetal-liver-and-fet-dcf62tre | Austin Chen, Mohsen Khosravi-Maharlooei, Markus Holzl, Nichole Danzl, Chris Parks, Elizabeth Waffarn, Megan Sykes | TITLE: Human CD34+ cell isolation from fetal liver, and fetal thymus preparation
AUTHORS: Austin Chen, Mohsen Khosravi-Maharlooei, Markus Holzl, Nichole Danzl, Chris Parks, Elizabeth Waffarn, Megan Sykes
[DESCRIPTION]
This protocol details the steps for isolating human CD34+ cells from human fetal liver. It also expla... | ["[Digestion method for CD34+ isolation] Start water bath and heat to 37 °C if not already on.", "[Digestion method for CD34+ isolation] Spray the fetal organ test tube before putting it into the hood", "[Digestion method for CD34+ isolation] Fill beaker w/ 70% EtOH. Soak your tools in this.", "[Digestion method for C... |
102,082 | TKI Diatom NGS Protocol | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8k4plmk/v1 | https://www.protocols.io/view/tki-diatom-ngs-protocol-dfxa3pie | Kevin Beentjes | TITLE: TKI Diatom NGS Protocol
AUTHORS: Kevin Beentjes
[DESCRIPTION]
This protocol describes a standardized workflow for the analysis of diatom samples using metabarcoding of the rbcL gene. It was created for the DNA Diatom Biosensor project funded by TKI Watertechnologie. More information on the project can be found ... | ["[Field Sampling] At the selected location, select approximately 5 to 10 reed stems, and cut them at a depth of 15 to 20 cm below the water surface. If there are no reeds present, other (water) plants can also be collected or hard substrate can be scraped off, provided it is in contact with the open water.\n\nStandard... |
95,588 | Blue Native PAGE of PINK1-TOM complex in mammalian cells | 1 | dx.doi.org/10.17504/protocols.io.5qpvo3mezv4o/v1 | https://www.protocols.io/view/blue-native-page-of-pink1-tom-complex-in-mammalian-c9kcz4sw | Hina Ojha, Miratul M. K. Muqit | TITLE: Blue Native PAGE of PINK1-TOM complex in mammalian cells
AUTHORS: Hina Ojha, Miratul M. K. Muqit
[DESCRIPTION]
Autosomal recessive mutations in PTEN-induced kinase 1 (PINK1) are associated with early-onset Parkinson's disease (PD) [1]. Upon inducing mitochondrial depolarization using uncouplers, the full-length... | ["[Formation of mitochondrial enriched pellet] For making mitochondrial enriched fraction first harvest the cells after giving desired treatments (doxycycline for PINK1 induction and Antimycin/Oligomycin for mitochondrial damage).", "[Formation of mitochondrial enriched pellet] For collection keep plates with cells on ... |
null | null | null | dx.doi.org/10.17504/protocols.io.fw6bphe | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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null | null | null | dx.doi.org/10.17504/protocols.io.cidua5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocols is for PCR using Q5® High-Fidelity DNA Polymerase (M0491)
[GUIDELINES]
<strong>Please note that protocols with Q5® High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended below should be used for optimal perfo... | [] |
90,566 | Populating NCBI template for submissions using BioNumerics | 1 | dx.doi.org/10.17504/protocols.io.36wgq3ejylk5/v1 | https://www.protocols.io/view/populating-ncbi-template-for-submissions-using-bio-c4peyvje | Ruth Timme, Maria Balkey, Julie Haendiges, Brian Sauders, Tina.Pfefer | TITLE: Populating NCBI template for submissions using BioNumerics
AUTHORS: Ruth Timme, Maria Balkey, Julie Haendiges, Brian Sauders, Tina.Pfefer
[DESCRIPTION]
PURPOSE: to define the standard operating procedure for collecting isolate metadata using BioNumerics for submission of food/environmental isolates to NCBI.
... | ["Metadata SampleSheet preparation \n\nBefore uploading your sequencing run or linking NCBI sequencing records at the BioNumerics platform make sure to fill out the metadata spreadsheet form. \n\nPlease download the template and guidelines included in the file \n\nCreate the fields NCBI_bioproject, Attribute_package... |
19,089 | Complications of CT-guided lung biopsy with a non-coaxial semi-automated 18 gauge biopsy system: frequency, severity and risk factors | null | dx.doi.org/10.17504/protocols.io.wvrfe56 | null | Amany Elshafee, Annika Karch, Kristina I. Ringe, Hoen-oh Shin, Hans-Jürgen Raatschen, Nermin Yehia Soliman, Frank Wacker, Jens Vogel-Claussen | TITLE: Complications of CT-guided lung biopsy with a non-coaxial semi-automated 18 gauge biopsy system: frequency, severity and risk factors
AUTHORS: Amany Elshafee, Annika Karch, Kristina I. Ringe, Hoen-oh Shin, Hans-Jürgen Raatschen, Nermin Yehia Soliman, Frank Wacker, Jens Vogel-Claussen
[DESCRIPTION]
<div class = ... | ["All consecutive CT-guided core lung biopsies recorded in our database from May 2011 to July 2014 were retrospectively analyzed. A total of 335 biopsies were assessed. Inclusion criteria included patients who underwent CT-guided lung biopsy with available images and reports.5 canceled biopsies were excluded from the a... |
107,998 | Hepatitis E and Enterovirus Multiplex Nested RT-PCR | 0 | dx.doi.org/10.17504/protocols.io.rm7vzjd74lx1/v1 | https://www.protocols.io/view/hepatitis-e-and-enterovirus-multiplex-nested-rt-pc-dmp645re | Shannon Fitz, Alex Shaw, Dilip Abraham, michael Owusu | TITLE: Hepatitis E and Enterovirus Multiplex Nested RT-PCR
AUTHORS: Shannon Fitz, Alex Shaw, Dilip Abraham, michael Owusu
[DESCRIPTION]
For multiplex, nested amplification of Hepatitis E (HEV) and Enterovirus (EV) samples.
Primer sets and target amplicon size
Outer HEV: HEV_38_f and HEV_39_r (538 bps)
HEV_38_f: GARG... | ["[Hepatitis E and Enterovirus Multiplex Nested PCR] First round PCR for combined enterovirus and hepatitis E samples", "[Hepatitis E and Enterovirus Multiplex Nested PCR] Prepare master mix on ice per number of samples/controls + 10%. Flick master mix gently to mix, and spin down. Aliquot 20 ul into each well, then ad... |
81,664 | Rodent intracerebroventricular AAV injections | 4 | dx.doi.org/10.17504/protocols.io.5qpvorm4dv4o/v1 | https://www.protocols.io/view/rodent-intracerebroventricular-aav-injections-cty8wpzw | Miguel Chuapoco | TITLE: Rodent intracerebroventricular AAV injections
AUTHORS: Miguel Chuapoco
[DESCRIPTION]
This abstract outlines the procedure for AAV injections into the lateral ventricle of C57BL/6J mice (intracerebroventricular injections)
[STEPS]
SECTION: Rodent surgery
1. Raise and house mice in compliance with your local Ins... | ["[Rodent surgery] Raise and house mice in compliance with your local Institutional Animal Care and Use Committee (IACUC) and ensure that all protocols and procedures have been approved by the appropriate ethical and regulatory committees. \n\nObtain mice from The Jackson Laboratory (male, 6-8 weeks).", "[Rodent surger... |
49,816 | 5 | 1 | null | https://www.protocols.io/view/5-buvynw7w | Ellen Smith | TITLE: 5
AUTHORS: Ellen Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">abstract</div></div>
[STEPS] | [] |
57,619 | Enhanced QIAseq DIRECT SARS-CoV-2 Kit for Illumina MiSeq | 4 | dx.doi.org/10.17504/protocols.io.b4htqt6n | https://www.protocols.io/view/enhanced-qiaseq-direct-sars-cov-2-kit-for-illumina-b4htqt6n | Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: Enhanced QIAseq DIRECT SARS-CoV-2 Kit for Illumina MiSeq
AUTHORS: Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
This SARS-CoV-2 targeted, a rapid viral RNA library preparation kit compatible with Illumina sequencers, provides an approximately five hour tur... | ["[cDNA Synthesis Procedure]", "[cDNA Synthesis Procedure] Thaw template RNA on ice. Gently yet thoroughly mix, then briefly centrifuge to collect residual liquid from the sides of the tubes and return to ice.", "[cDNA Synthesis Procedure] While keeping reagents on ice, prepare the cDNA reaction according to the table ... |
43,566 | Multitrim | 4 | null | https://www.protocols.io/view/multitrim-bnsnmede | Kenji Gerhardt, Kostas Konstantinidis | TITLE: Multitrim
AUTHORS: Kenji Gerhardt, Kostas Konstantinidis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Multitrim is an Illumina short read trimming pipeline which facilitates effectively and easily trimming either single end or paired end FASTQ data. Multitrim achieves high quality trimming... | ["All contents of this protocol are best viewed in either the attached manual , or by going to the Multitrim user manual google doc. You can also follow the instructions on this page to achieve the same results..", "Install the correct version of Anaconda or Miniconda for Python 3.6+ for your system, if it is not alre... |
13,910 | Procedure to extract DNA from peripheral blood with FlexiGene DNA kit (Qiagen) and genotyping genomic DNA. | null | dx.doi.org/10.17504/protocols.io.rtwd6pe | null | Raul Escamilla, Beatriz Camarena, Sandra Hernández-Muñoz, Alejandro Aguliar-García | TITLE: Procedure to extract DNA from peripheral blood with FlexiGene DNA kit (Qiagen) and genotyping genomic DNA.
AUTHORS: Raul Escamilla, Beatriz Camarena, Sandra Hernández-Muñoz, Alejandro Aguliar-García
[DESCRIPTION]
<p>To describe the procedure to extract DNA from peripheral blood with FlexiGene DNA kit (Qiagen), ... | ["1) Extract DNA from buffy coat (FLEXIGENE KIT (Qiagen), follow basically manufacturer’s instructions)a) Obtain 5 ml of peripheral blood in vacutainer tubes with EDTA from each patient.", "c) Transfer the buffy coat into a 2 ml O-ring Tube with the help of a disposable 3 ml transference pipette.", "d) Pipet 1250 µl ... |
47,716 | McKenzie Lab 16S/ITS Sample Processing Protocol | 3 | dx.doi.org/10.17504/protocols.io.bsucnesw | https://www.protocols.io/view/mckenzie-lab-16s-its-sample-processing-protocol-bsucnesw | Alexandra Alexiev | TITLE: McKenzie Lab 16S/ITS Sample Processing Protocol
AUTHORS: Alexandra Alexiev
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is the protocol used in the McKenzie Lab at CU Boulder for 16S and ITS1 sample processing for eventual amplicon sequencing on a MiSeq.</div></div>
[STEPS] | [] |
71,603 | 5% Albumin compared with Balanced Crystalloid, as intravenous fluid resuscitation in adult patients with sepsis, presenting as an emergency to hospital: Statistical Analysis Plan for the randomised controlled feasibility study, “ABC-Sepsis” | 1 | dx.doi.org/10.17504/protocols.io.14egn276mg5d/v1 | https://www.protocols.io/view/5-albumin-compared-with-balanced-crystalloid-as-i-ch6tt9en | Roz Pollock, Christopher Weir, John Norrie, Alasdair J Gray | TITLE: 5% Albumin compared with Balanced Crystalloid, as intravenous fluid resuscitation in adult patients with sepsis, presenting as an emergency to hospital: Statistical Analysis Plan for the randomised controlled feasibility study, “ABC-Sepsis”
AUTHORS: Roz Pollock, Christopher Weir, John Norrie, Alasdair J Gray
[... | ["Introduction\n\nThis trial will compare 5% Human Albumin Solution (HAS) with standard care (balanced crystalloid) as intravenous fluid resuscitation in adult patients with community acquired sepsis presenting to the emergency department (ED). The aims are to investigate:\n\n the feasibility of being able to recruit a... |
90,816 | Preparation and Administration of Systemic 4-AP | 1 | dx.doi.org/10.17504/protocols.io.5qpvo38dxv4o/v1 | https://www.protocols.io/view/preparation-and-administration-of-systemic-4-ap-c4w8yxhw | Leonardo A Parra-Rivas | TITLE: Preparation and Administration of Systemic 4-AP
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
Preparation and Administration of Systemic 4-AP
[STEPS]
1.
4-Aminopyridine was obtained from Sigma-Aldrich (Cat #A78403), and a stock solution was
prepared in saline. Mice (8 weeks old) were given intraperitoneal ... | ["4-Aminopyridine was obtained from Sigma-Aldrich (Cat #A78403), and a stock solution was\nprepared in saline. Mice (8 weeks old) were given intraperitoneal injections of\nsoluble 4-AP at a low dose of 2 mg/Kg or equivalent saline.", "Animals were monitored every 20 min and presented no sign of convulsive seizure behav... |
83,833 | Basic maintenance protocol for human induced pluripotent stem cell (hIPSCs) | 4 | dx.doi.org/10.17504/protocols.io.81wgbx1q1lpk/v1 | https://www.protocols.click/view/basic-maintenance-protocol-for-human-induced-pluri-cv4zw8x6 | William J Buchser, Mallory Wright, purva patel, jwaligor, ckremitz | TITLE: Basic maintenance protocol for human induced pluripotent stem cell (hIPSCs)
AUTHORS: William J Buchser, Mallory Wright, purva patel, jwaligor, ckremitz
[DESCRIPTION]
Human Induced pluripotent stem cell (hIPSCs) maintenance protocol. This includes thawing, passaging, performing a media change, single cell dissoc... | ["[Thawing iPSCs] Quickly thaw the frozen vial in a 37 °C water bath 2 min", "[Thawing iPSCs] Using a 10 ml serological pipette, add 9 mL of media into 15 ml conical tube.", "[Thawing iPSCs] Add the contents of the cryovial into the 15 ml conical tube with a 5 ml serological pipette", "[Thawing iPSCs] Spin down conical... |
86,577 | Evercode WT Mega v2.2.1 | 4 | null | https://www.protocols.io/view/evercode-wt-mega-v2-2-1-cysrxwd6 | Parse Biosciences, Elisabeth Rebboah | TITLE: Evercode WT Mega v2.2.1
AUTHORS: Parse Biosciences, Elisabeth Rebboah
[DESCRIPTION]
This protocol describes the original Parse Biosciences Evercode WT Mega v2 protocol for single-nucleus or single-cell RNA-seq of 1,000,000 nominal nuclei or cells. Unlike other scRNA-seq methods that physically separate individu... | ["[Section 1: Barcoding Single Cells] 1.1: Experimental Setup", "[Section 1: Barcoding Single Cells] Prepare for the first round of barcoding with the following checklist:\nEach sample should have been counted after nuclei fixation and recorded on the spreadsheet in order to calculate volumes to normalize concentration... |
34,589 | Single-Cell ICN Neuron Mapping and 3D Heart Reconstruction with Tissue Mapper | 1 | dx.doi.org/10.17504/protocols.io.bdz5i786 | https://www.protocols.io/view/single-cell-icn-neuron-mapping-and-3d-heart-recons-bdz5i786 | Clara Leung, Maci Heal, Shaina Robbins, Alison Moss, Corey Monteith, Susan Tappan | TITLE: Single-Cell ICN Neuron Mapping and 3D Heart Reconstruction with Tissue Mapper
AUTHORS: Clara Leung, Maci Heal, Shaina Robbins, Alison Moss, Corey Monteith, Susan Tappan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the process of using the Tissue Mapper software to m... | ["[Software installation setup]\nHave Tissue Mapper installed and licensed on your workstation of choice through an MBF Bioscience representative.", "[Cell marking and image annotation]\nMetadata setup. To begin, create an empty document by filling in the subject information of your sample with the species, sample ID, ... |
null | null | null | dx.doi.org/10.17504/protocols.io.eqybdxw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
A simple procedure to conjugate plasmids between bacteria. Conjugation is performed on sterile filters to reduce the number of plates required (5 can easily be fit on one 10 cm plate) and to make suspending the bacteria in liquid simple following conjugation.
[BEFORE_START]
Don... | [] |
40,087 | DNA extraction and Sequencing | 3 | dx.doi.org/10.17504/protocols.io.bjdxki7n | https://www.protocols.io/view/dna-extraction-and-sequencing-bjdxki7n | avinash.kale | TITLE: DNA extraction and Sequencing
AUTHORS: avinash.kale
[STEPS] | [] |
29,355 | RA STRAIN SUPPLEMENT | null | dx.doi.org/10.17504/protocols.io.8wjhxcn | null | Manuel Richter | TITLE: RA STRAIN SUPPLEMENT
AUTHORS: Manuel Richter
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">RA STRAIN SUPPLEMENT</div></div>
[STEPS]
?. | [] |
78,837 | 2023 GenomeTrakr Proficiency Testing exercise (PulseNet Harmonized) | 1 | dx.doi.org/10.17504/protocols.io.e6nvw5m9dvmk/v6 | https://www.protocols.io/view/2023-genometrakr-proficiency-testing-exercise-puls-cq8vvzw6 | Maria Balkey, Ruth Timme, Julie Haendiges, Tina.Pfefer | TITLE: 2023 GenomeTrakr Proficiency Testing exercise (PulseNet Harmonized)
AUTHORS: Maria Balkey, Ruth Timme, Julie Haendiges, Tina.Pfefer
[DESCRIPTION]
Version Updates
v5: Added links to GalaxyTrakr_PT_exercise_report_2022.xlsx
v6: added 2023 PT strains; edited Steps 1, 2, 4, and 5 to include references to growth ... | ["[Culture Preparation] Salmonella, Escherichia/Shigella, and Listeria Lyophilized cultures:\n\nDay 1\n\nDocument the isolate number(s) and the lyophilized date(s) for your records. Wipe the aluminum cover and outside of the vial with isopropyl alcohol. Using sturdy forceps, aseptically remove the aluminum cover and r... |
85,010 | Testimony on a successful lab protocol to disrupt Chlorella vulgaris microalga cell wall | 1 | dx.doi.org/10.17504/protocols.io.dm6gpb9z5lzp/v2 | https://www.protocols.click/view/testimony-on-a-successful-lab-protocol-to-disrupt-cw9sxh6e | Diogo Coelho, Paula A. Lopes, José A. M. Prates | TITLE: Testimony on a successful lab protocol to disrupt Chlorella vulgaris microalga cell wall
AUTHORS: Diogo Coelho, Paula A. Lopes, José A. M. Prates
[DESCRIPTION]
Over the last decades, microalgae have gained popularity due to demand for novel environmental green solutions and development of innovative mass-produc... | ["[Construction of the CAZymes bank and production of microalgae Incubation of C. vulgaris suspension with individual CAZymes (glycoside hydrolases, glycosyl transferases, polysaccharide lyases and carbohydrate esterases)] Preparation of C. vulgaris suspension (20 mg/mL) resuspending the microalgae powder in PBS buffer... |
60,202 | Expression of molecular markers in mouse and human stellate ganglia | 4 | dx.doi.org/10.17504/protocols.io.rm7vzyko5lx1/v1 | https://www.protocols.io/view/expression-of-molecular-markers-in-mouse-and-human-b62irgce | Daniele Neri, Seoeun Lee, Alexandre J Lafond, Lori Zeltser | TITLE: Expression of molecular markers in mouse and human stellate ganglia
AUTHORS: Daniele Neri, Seoeun Lee, Alexandre J Lafond, Lori Zeltser
[DESCRIPTION]
Protocol for immunohistochemistry
[BEFORE_START]
The human stellate ganglia (SG) were provided by Dr. Olujimi Ajijola at UCLA. The tissue was collected po... | ["[Postfixation of the tissue] Both mouse and human stellate ganglia (SG) were fixed in 4% PFA in 0.1M PB overnight at 4°C, washed with Phosphate Buffer Saline (PBS) 4°C for at least 1 h", "[Encasing and slicing] Ganglia were encased in Optimal Cutting Temperature compound (Tissue-Tek) cut into 10 μm cryo-sections on s... |
76,151 | Isolation of West Nile virus on Vero cell lines | 1 | dx.doi.org/10.17504/protocols.io.14egn2e7pg5d/v1 | https://www.protocols.io/view/isolation-of-west-nile-virus-on-vero-cell-lines-cnkxvcxn | Anna Nagy | TITLE: Isolation of West Nile virus on Vero cell lines
AUTHORS: Anna Nagy
[DESCRIPTION]
West Nile virus (WNV), a member of the family Flaviviridae, genus Flavivirus is a mosquito-borne emerging pathogen, which is endemic in most part of Europe, especially in the central and southern regions of the continent. The vast... | ["[WNV isolation from PCR positive clinical samples:] Cell lines: Vero or Vero E6, that are suitable for West Nile virus. Cell culture should be grown in T25 or T75 cell culture flasks.\n- Cell culture medium: DMEM (Dulbecco's Modified Eagle Medium) high (4,5 g/L) glucose with L-Glutamine 1000L (Lonza; Catalog #: BE15-... |
79,369 | Immunolabeling of a Whole Mouse Brain | 1 | dx.doi.org/10.17504/protocols.io.ewov1okwylr2/v1 | https://www.protocols.click/view/immunolabeling-of-a-whole-mouse-brain-crrhv536 | Andrew Recknagel, Kevin Cao, Molly Logsdon, Judith Baka, Naveen Ouellette, Jayaram Chandrashekar | TITLE: Immunolabeling of a Whole Mouse Brain
AUTHORS: Andrew Recknagel, Kevin Cao, Molly Logsdon, Judith Baka, Naveen Ouellette, Jayaram Chandrashekar
[DESCRIPTION]
Our mice have sparse viral labeling of target neuron populations with genetically encoded fluorescent proteins (FPs). Delipidation ensures that whole brai... | ["[Immunolabeling of a Whole Mouse Brain] Transfer the brain to a small 4 mL vial containing diluted primary antibody in ~ 4 mL PTxw and incubate with rocking, a nutator or shaker, at Room temperature for 11 days. \n10 μg/brain for primary antibody\n20 μg/brain for primary conjugated antibody", "[Immunolabeling of a Wh... |
null | null | null | dx.doi.org/10.17504/protocols.io.dy27yd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
The viral concentrate used for PFGE analysis must be molded into plugs, followed by lysis of the virus particles to release their DNA. It is possible to run solution-based preparation of viral DNA for PFGE (Steward 2001); however, large DNA molecules (>100 kb) are extremely s... | [] |
26,544 | UC Davis - Immunohistochemistry Factor VIII | null | dx.doi.org/10.17504/protocols.io.56qg9dw | null | Jennifer Rutkowsky | TITLE: UC Davis - Immunohistochemistry Factor VIII
AUTHORS: Jennifer Rutkowsky
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Factor VIII is a glycoprotein present in human plasma, human endothelial cells, megakaryocy... | ["Immunohistochemistry was performed on four-micron thick, formalin-fixed, paraffin-embedded tissue sections, mounted on charged slides, and air-dried overnight at 37º C.", "Sections were deparaffinized through xylene to 100% reagent alcohol, and then treated with 0.3% hydrogen peroxide in 100% methanol for 30 minutes.... |
null | null | null | dx.doi.org/10.17504/protocols.io.iwbcfan | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p>This cleaning protocol is meant to be used when there is an excess of particulate buildup in the FCM. Blockages can be detected by the failure of the FCM to... | [] |
64,436 | Regal Keto Gummies [REAL or HOAX] Risky User Complaints List 2022? | 3 | dx.doi.org/10.17504/protocols.io.yxmvmnm66g3p/v1 | https://www.protocols.io/view/regal-keto-gummies-real-or-hoax-risky-user-complai-ca6ushew | G A | TITLE: Regal Keto Gummies [REAL or HOAX] Risky User Complaints List 2022?
AUTHORS: G A
[DESCRIPTION]
Large numbers of these enhancements have traveled every which way, so what makes Regal KETO exceptional? We can state very little with respect to its uniqueness in light of the fact that:
[STEPS] | [] |
86,049 | Immunofluorescence Staining | 1 | dx.doi.org/10.17504/protocols.io.j8nlkowddv5r/v1 | https://www.protocols.io/view/immunofluorescence-staining-cx99xr96 | Toby J Curless, Hemanth Ramesh Nelvagal, Zane Jaunmuktane | TITLE: Immunofluorescence Staining
AUTHORS: Toby J Curless, Hemanth Ramesh Nelvagal, Zane Jaunmuktane
[DESCRIPTION]
The protocol describes immunofluorescence staining on brain sections.
[STEPS]
SECTION: Preparation
1. Generate tissue sections using standard microtome sectioning protocols.
SECTION: Preparation
2. Hea... | ["[Preparation] Generate tissue sections using standard microtome sectioning protocols.", "[Preparation] Heat tissue dry tissue sections for 60 min at 60 °C", "[De-paraffinisation and Immunofluorescence] De-paraffinise sections by 5 min min washes in xylene (x3), 5 min 100 % ethanol (x1), 5 min 70 % ethanol (x1) and 5... |
99,218 | Database-driven protein solubilization | 0 | dx.doi.org/10.17504/protocols.io.36wgqn735gk5/v1 | https://www.protocols.io/view/database-driven-protein-solubilization-dc5s2y6e | Caroline Brown, Snehasish Ghosh, Kallol Gupta | TITLE: Database-driven protein solubilization
AUTHORS: Caroline Brown, Snehasish Ghosh, Kallol Gupta
[DESCRIPTION]
This is a protocol for using the extraction database found at www.polymerscreen.yale.edu for optimal extraction of membrane proteins into native nanodiscs.
[STEPS]
1. Choose your protein of interest.
2. ... | ["Choose your protein of interest.", "Visit www.polymerscreen.yale.edu to search for your protein in the extraction database. This will provide you with the polymer to use for optimal protein extraction.", "As an example, below are representative organellar markers and the optimal polymer for extraction:\nTMEM192 --> A... |
67,421 | Growth Matrix Male Enhancement - What Results Can You Expect By Using | 3 | dx.doi.org/10.17504/protocols.io.ewov1npyogr2/v1 | https://www.protocols.io/view/growth-matrix-male-enhancement-what-results-can-yo-cd35s8q6 | saksemahiano | TITLE: Growth Matrix Male Enhancement - What Results Can You Expect By Using
AUTHORS: saksemahiano
[DESCRIPTION]
https://google-news.clubeo.com/page/growth-matrix-male-enhancement-what-results-can-you-expect-by-using.html
[STEPS] | [] |
28,007 | Golden Gate Assembly (Protocol for NEB® Golden Gate Assembly Mix) | null | dx.doi.org/10.17504/protocols.io.7kfhktn | null | Alba Balletbó | TITLE: Golden Gate Assembly (Protocol for NEB® Golden Gate Assembly Mix)
AUTHORS: Alba Balletbó
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Golden Gate Assembly and its derivative methods exploit the ability of Type IIS restriction endonucleases (REases) to cleave DNA outside of the recognition ... | ["[Assembly Reactions]\nSet up 25 µl assembly reactions as follows: ABC1Assembly ReactionNegative Control (If desired)2pGGA Destination Plasmid*, 75 ng/μl1 μl (75 ng)1 μl (75 ng)324 precloned inserts cloned into pMiniT 2.0, 100 ng/ul each plasmid0.75 µl (75 ng) each, (18 µl total)-4T4 DNA Ligase Buffer (10X)2.5 μl2.5 ... |
null | null | null | dx.doi.org/10.17504/protocols.io.evgbe3w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes the procedures for making a 1.5 mol L<sup>-1</sup> nitrite reagent for measuring total dissolved and labile cobalt using cathodic stripping voltammetry.
[STEPS]
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.rs7d6hn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This was a past assay that we no longer in use. For our favoured Measles TaqMan test, please refer to the <em>MeV N TaqMan</em> protocol.</p>
<p>The protocol aims to amplify measles virus (MeV) strains and not other viruses.</p>
<p>Michael Lyon designed the original version o... | [] |
99,223 | Serapure Preparation and Testing | 1 | null | https://www.protocols.io/view/serapure-preparation-and-testing-dc5x2y7n | Andreas Novotny | TITLE: Serapure Preparation and Testing
AUTHORS: Andreas Novotny
[DESCRIPTION]
A protocol template created through the BeBOP project for DNA Extraction.
[BEFORE_START]
Read background information, MIOP and BePOP-OBON information under the "Guidelines" tab.
[GUIDELINES]
MIOP: Minimum Information about an Omics Proto... | ["[STANDARD OPERATING PROCEDURE] In the following SOP, please use the exact names of equipment as noted in the table above.\n\nProvide a step-by-step description of the protocol. The identification of difficult steps in the protocol and the provision of recommendations for the execution of those steps are encouraged.",... |
null | null | null | dx.doi.org/10.17504/protocols.io.i59cg96 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Transmission of bacteria from inanimate surfaces in healthcare associated environments is an important source of hospital acquired infections. A number of commercially available medical devices promise to fulfill antibacterial activity to reduce environmental contamination. U... | [] |
21,636 | Vandy – Bile Diversion in Mice | null | dx.doi.org/10.17504/protocols.io.zdcf22w | null | Vance L. Albaugh | TITLE: Vandy – Bile Diversion in Mice
AUTHORS: Vance L. Albaugh
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This is the protocol for conducting the bile diversion procedure in the mouse. In this procedure the commo... | ["Preoperative Care 1. All animals must be singly-housed. 2. Preoperative pain medications should be administered: a. Ketoprofen (5 mg/kg) b. Buprenorphine (0.05 mg/kg) c. LRS are given at the end of surgery and a second dose is given 24 hours later. 3. Ensure adequacy of anesthesia. 4. Place mouse on surgi... |
40,279 | Assembly of plasmids for LacI genotype-phenotype landscape measurement | 3 | dx.doi.org/10.17504/protocols.io.bjjxkkpn | https://www.protocols.io/view/assembly-of-plasmids-for-laci-genotype-phenotype-l-bjjxkkpn | Drew S Tack, Nina Alperovich, Olga Vasilyeva, David Ross | TITLE: Assembly of plasmids for LacI genotype-phenotype landscape measurement
AUTHORS: Drew S Tack, Nina Alperovich, Olga Vasilyeva, David Ross
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Here, we describe protocols for assembly of two different plasmids used for the measurement of the g... | [] |
40,411 | Target Guide Sequence Cloning Protocol Version 2 | 4 | dx.doi.org/10.17504/protocols.io.bjp3kmqn | https://www.protocols.io/view/target-guide-sequence-cloning-protocol-version-2-bjp3kmqn | Skye Waterland, Yang Li | TITLE: Target Guide Sequence Cloning Protocol Version 2
AUTHORS: Skye Waterland, Yang Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Create single gRNA vectors for targeted cloning utilizing CRISPR or CRISPR-based systems. </div></div>
[STEPS]
?. [Lentiviral vector digestion]
Digest and dephosp... | ["[Lentiviral vector digestion]\nDigest and dephosphorylate", "[Lentiviral vector digestion]\nAdd to a .\n40 µl\n1.5 mL", "[Lentiviral vector digestion]\nAdd of to solution.\n5 µl", "[Lentiviral vector digestion]\nAdd of to solution.\n3 µl\n*Note: we used BsmBI Cat #R0580, NEB, but this one has been discontinued. #... |
84,360 | High-Molecular-Weight SPRI-aided DNA extraction from Mimulus (Phrymaceae) leaf tissue | 4 | dx.doi.org/10.17504/protocols.io.bp2l6xn8rlqe/v1 | https://www.protocols.click/view/high-molecular-weight-spri-aided-dna-extraction-fr-cwmgxc3w | Bolívar Aponte Rolón | TITLE: High-Molecular-Weight SPRI-aided DNA extraction from Mimulus (Phrymaceae) leaf tissue
AUTHORS: Bolívar Aponte Rolón
[DESCRIPTION]
This protocol is adapted from Russo et al. 2023 methods for high-molecular weight DNA extraction. I modified the protocol to use reagents and incubation conditions used by the Ferris... | ["[Sample preparation] Clean the biosafety cabinet, pipettes, tip boxes and any other instrument to be used with 70% EtOH, 10 % Bleach and 95 % EtOH. Surface sterilize all instruments in biosafety cabinet with UV light for 30 min.", "[Sample preparation] Quickly after placing tissue in tubes, place tube cap and close ... |
26,050 | Surgical Removal of Sea Cucumber Gonads | null | dx.doi.org/10.17504/protocols.io.5pag5ie | null | Jon Eilers | TITLE: Surgical Removal of Sea Cucumber Gonads
AUTHORS: Jon Eilers
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol for sea cucumber gonad removal without killing the sea cucumber or causing evisceration. </div></div>
[STEPS]
?. Anesthetize Sea Cucumber as described in the Sea Cuc... | ["Anesthetize Sea Cucumber as described in the Sea Cucumber Anesthetizing protocol: dx.doi.org/10.17504/protocols.io.5nyg5fw", "Locate the gonadopore just posterior to the head of the sea cucumber and pinch the skin just behind it", "Using the pointed side of the dissection scissors, push into the body wall of the sea ... |
95,260 | LUHMES (lund human mesencephalic) culturing and differentiation protocol | 4 | dx.doi.org/10.17504/protocols.io.kxygx36ykg8j/v2 | https://www.protocols.io/view/luhmes-lund-human-mesencephalic-culturing-and-diff-c894zz8w | Mallory Wright, William J Buchser, ckremitz, jwaligor, bachman@wustl.edu | TITLE: LUHMES (lund human mesencephalic) culturing and differentiation protocol
AUTHORS: Mallory Wright, William J Buchser, ckremitz, jwaligor, bachman@wustl.edu
[STEPS]
SECTION: LUHMES coating protocol
1.
0.1 mg/mL
50 µg/µL
Thaw an aliquot of Poly-L-Ornithine solution at room temperature.
SECTION: LUHMES Passaging ... | ["[LUHMES coating protocol] 0.1 mg/mL\n50 µg/µL\nThaw an aliquot of Poly-L-Ornithine solution at room temperature.", "[LUHMES Passaging (~ every 2-3 days)] use a 5mL pipette to Triturate cells only 1 or 2 times before seeding.", "[LUHMES Passaging (~ every 2-3 days)] Discard the supernatant and resuspend in 1mL LUHMES ... |
64,416 | Simpli Keto + ACV Gummies {Scam Exposed} Is It Scam? | 3 | dx.doi.org/10.17504/protocols.io.5qpvobwzxl4o/v1 | https://www.protocols.io/view/simpli-keto-acv-gummies-scam-exposed-is-it-scam-ca58sg9w | JosephDHedberg | TITLE: Simpli Keto + ACV Gummies {Scam Exposed} Is It Scam?
AUTHORS: JosephDHedberg
[DESCRIPTION]
Simpli ACV Keto Gummies advanced weight loss product assists in the natural reduction of hunger and stimulating fat reduction and the effects are visible after one week of use.
[STEPS] | [] |
85,984 | SARS-CoV-2 Whole Genome Amplicon Sequencing from Liquid Wastewater | 4 | dx.doi.org/10.17504/protocols.io.yxmvm323bl3p/v1 | https://www.protocols.io/view/sars-cov-2-whole-genome-amplicon-sequencing-from-l-cx78xrrw | Vikram Chan-Herur, David Catoe, Dorothea Duong, Yi-Chieh Wu, Alexandria B Boehm, Bradley J White, Marlene K Wolfe | TITLE: SARS-CoV-2 Whole Genome Amplicon Sequencing from Liquid Wastewater
AUTHORS: Vikram Chan-Herur, David Catoe, Dorothea Duong, Yi-Chieh Wu, Alexandria B Boehm, Bradley J White, Marlene K Wolfe
[DESCRIPTION]
This process instruction describes steps for whole genome amplicon sequencing of SARS-CoV-2 from wastewater,... | ["[Viral Particle Concentration] Spin down 50 mL of influent at 5,250 xg for 5 min to clarify the influent.", "[Viral Particle Concentration] Add 4.875 mL of influent sample to one well (one well per sample) of a new KingFisher 24 Well Deep Well Plate.", "[Viral Particle Concentration] Add another 4.875 mL of influent ... |
97,516 | CODA: shorthand for calling functions | HuBMAP | JHU-TMC | 0 | dx.doi.org/10.17504/protocols.io.5qpvoknkbl4o/v1 | https://www.protocols.io/view/coda-shorthand-for-calling-functions-hubmap-jhu-tm-dbgk2juw | Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz | TITLE: CODA: shorthand for calling functions | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz
[DESCRIPTION]
To downsample ndpi or svs images to 10x, 5x, and 1x tifs, use this function:
create_downsampled_tif_images
or try Openslide in pytho... | [] |
70,864 | Obtain LB broth medium | 4 | dx.doi.org/10.17504/protocols.io.5qpvoryxdv4o/v1 | https://www.protocols.io/view/obtain-lb-broth-medium-chfqt3mw | An.Huang | TITLE: Obtain LB broth medium
AUTHORS: An.Huang
[DESCRIPTION]
LB broth medium is a basic culture media for microorganisms. This protocol helps make an ordinary LB broth medium.
[STEPS]
1. Dissolve 25 gLB broth powder in 1000 mL distilled water.
2. Autoclave the medium.
3. Store the medium at 4 °C.
| ["Dissolve 25 gLB broth powder in 1000 mL distilled water.", "Autoclave the medium.", "Store the medium at 4 °C."] |
100,896 | DNA Extraction from 0.22µm Sterivex Filters - Qiagen Blood and Tissue Kit | 1 | dx.doi.org/10.17504/protocols.io.bp2l6221kgqe/v1 | https://www.protocols.io/view/dna-extraction-from-0-22-m-sterivex-filters-qiagen-der83d9w | Colleen Kellogg, rute.carvalho Carvalho, Matt Lemay | TITLE: DNA Extraction from 0.22µm Sterivex Filters - Qiagen Blood and Tissue Kit
AUTHORS: Colleen Kellogg, rute.carvalho Carvalho, Matt Lemay
[DESCRIPTION]
This protocol is used to extract genomic DNA from 0.22μm sterivex filters using Quiagens Blood and Tissue Kit. The protocol was developed as an equivalent replacem... | ["[PREPARATION - must be done in Clean Room 1 or Clean Room 2] This protocol assumes that samples have been collected and preserved according to this procedure:", "[PREPARATION - must be done in Clean Room 1 or Clean Room 2] Set aside ATL buffer, AL buffer, proteinase K, RNase A and anhydrous ethanol. \nPut the ATL and... |
43,110 | Atomic Force Microscopy of DNA and DNA-Protein Interactions | 2 | dx.doi.org/10.17504/protocols.io.bncemate | https://www.protocols.io/view/atomic-force-microscopy-of-dna-and-dna-protein-int-bncemate | Philip J. Haynes, Kavit H. S. Main, Alice Pyne | TITLE: Atomic Force Microscopy of DNA and DNA-Protein Interactions
AUTHORS: Philip J. Haynes, Kavit H. S. Main, Alice Pyne
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometre resolution... | [] |
65,116 | Monkeypox virus multiplexed PCR amplicon sequencing (PrimalSeq) V.1. | 1 | dx.doi.org/10.17504/protocols.io.5qpvob1nbl4o/v1 | https://www.protocols.io/view/monkeypox-virus-multiplexed-pcr-amplicon-sequencin-cbt4snqw | Nicholas F.G. Chen*, Luc Gagne*, Matthew Doucette, Sandra Smole, Erika Buzby, Joshua Hall, Stephanie Ash, Rachel Harrington, Seana Cofsky, Selina Clancy, Curtis J Kapsak, Joel Sevinsky, Kevin Libuit, Chrispin Chaguza, Nathan D. Grubaugh, Daniel J. Park, Glen R. Gallagher#, Chantal B.F. Vogels# | TITLE: Monkeypox virus multiplexed PCR amplicon sequencing (PrimalSeq) V.1.
AUTHORS: Nicholas F.G. Chen*, Luc Gagne*, Matthew Doucette, Sandra Smole, Erika Buzby, Joshua Hall, Stephanie Ash, Rachel Harrington, Seana Cofsky, Selina Clancy, Curtis J Kapsak, Joel Sevinsky, Kevin Libuit, Chrispin Chaguza, Nathan D.... | ["[Amplicon Generation] Reagents:", "[Amplicon Generation] In two separate tubes, prepare the following master mixes:", "[Amplicon Generation] Label two sets of PCR tubes/plates for Pool 1 and Pool 2", "[Amplicon Generation] Add the following: \n20 µL Pool 1 master mix to each Pool 1 tube/well\n20 µL Pool 2 master mix ... |
46,724 | Enzymatic Ethanol Assay | 4 | dx.doi.org/10.17504/protocols.io.brvcm62w | https://www.protocols.io/view/enzymatic-ethanol-assay-brvcm62w | Sarah Hammer, Dev Kapadia, Shuen Hon, Marybeth Maloney, Daniel Olson, Lee Lynd | TITLE: Enzymatic Ethanol Assay
AUTHORS: Sarah Hammer, Dev Kapadia, Shuen Hon, Marybeth Maloney, Daniel Olson, Lee Lynd
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes a 96-well-plate-based, enzymatic assay for reliably estimating ethanol concentrations in experiment... | ["[Preparation]\nMake glycine bufferSodium pyrophosphate33.3 g/L3.333 gGlycine1.67 g/L0.167 gSemicarbazide hydrochloride0.125 g/L12.5 mgHydrochloric acid (1N)---To pH 9.0Water---Up to 100 mLStore at for up to 1 month.\nSodium pyrophosphate33.3 g/L3.333 gGlycine1.67 g/L0.167 gSemicarbazide hydrochloride0.125 g/L12.5 m... |
79,905 | Conexão do actígrafo ao computador - ActTrust | 5 | dx.doi.org/10.17504/protocols.io.x54v9jbepg3e/v3 | https://www.protocols.io/view/conex-o-do-act-grafo-ao-computador-acttrust-cr99v996 | Daniel Vartanian | TITLE: Conexão do actígrafo ao computador - ActTrust
AUTHORS: Daniel Vartanian
[DESCRIPTION]
Este protocolo faz parte do processo de coleta de dados actigráficos do Grupo Interdisciplinar de Pesquisa em Ritmos Biológicos (GIPERBIO). Ele foi desenhado para os actígrafos provenientes da companhia Condor Instruments. Alg... | ["Abra o software ActStudio.", "Acesse a tela Controle.", "Conecte o ActDock (base de conexão) no computador.", "Conecte o actígrafo no ActDock."] |
23,606 | DNA Extraction from Urine | null | dx.doi.org/10.17504/protocols.io.3awgife | null | Bing Yang | TITLE: DNA Extraction from Urine
AUTHORS: Bing Yang
[STEPS]
?. | [] |
36,023 | Preparation of 0.2M EDTA, pH 8.0 | null | dx.doi.org/10.17504/protocols.io.bfexjjfn | https://www.protocols.io/view/preparation-of-0-2m-edta-ph-8-0-bfexjjfn | Nicola O'Reilly | TITLE: Preparation of 0.2M EDTA, pH 8.0
AUTHORS: Nicola O'Reilly
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Purpose of examination / Clinical relevance</span></div><div class = "text-block">At the end of 2019, several pneumonia cases were reported in Wuhan, Chi... | ["Please select between the recipes for 1 L 0.2 M EDTA pH 8.0 and 2 L 0.2 M EDTA pH 8.0.\nWork in fume hood!", "Weigh out , dihydrate and add to a 1 L beaker.\n[EDTA disodium salt]", "Measure out and add to the beaker.\n[MilliQ water]", "Add a magnetic flea and place on a magnetic stirring plate to mix the solution. T... |
61,469 | X DOWNLOAD LG V20™ Software Update | 1 | dx.doi.org/10.17504/protocols.io.ewov1nzkogr2/v1 | https://www.protocols.io/view/x-download-lg-v20-software-update-b795rr86 | lg- | TITLE: X DOWNLOAD LG V20™ Software Update
AUTHORS: lg-
[DESCRIPTION]
Verizon Wireless is pleased to announce a software update for your device. This software update has been tested to help optimize device performance, resolve known issues and apply the latest security patches.
[STEPS]
SECTION: X DOWNLOAD LG V20™ So... | ["[X DOWNLOAD LG V20™ Software Update]", "[X DOWNLOAD LG V20™ Software Update] Connect your device to a Wi-Fi network, or make sure it has a strong Verizon Wireless network connection.", "[X DOWNLOAD LG V20™ Software Update] Ensure your battery is fully charged before starting the software update. System Update 14 ... |
40,925 | ELISA for quantification of human C4 in serum or plasma. | 6 | dx.doi.org/10.17504/protocols.io.bj75krq6 | https://www.protocols.io/view/elisa-for-quantification-of-human-c4-in-serum-or-bj75krq6 | Angel Justiz-Vaillant, Belkis Ferrer-Cosme | TITLE: ELISA for quantification of human C4 in serum or plasma.
AUTHORS: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. An anti-human C4 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.
?... | ["An anti-human C4 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.", "Add 50 µl of human serum or plasma. Human C4 present in the serum or plasma binds to antibodies adsorbed into the microwells.", "The microplate is blocked with 3% non-fat milk-PBS buf... |
69,683 | qPCR assay for detecting Triturus cristatus | 4 | dx.doi.org/10.17504/protocols.io.n92ldpbo7l5b/v1 | https://www.protocols.io/view/qpcr-assay-for-detecting-triturus-cristatus-cgattsen | Alexander Eiler, Omneya Osman | TITLE: qPCR assay for detecting Triturus cristatus
AUTHORS: Alexander Eiler, Omneya Osman
[DESCRIPTION]
eDNA assay to detect great crested newt Triturus cristatus
[GUIDELINES]
Laboratory work space and equipment were sterilized by UV-light and DNase solution and 70% ethanol. Filter pipet tips were used in all steps o... | ["[DNA extraction] DNA extraction was performed using Qiagen DNeasy power water sterivex kit. The quality of the extracted DNA was estimated using Nanodrop. Qiagen DNeasy power water sterivex kit: https://www.qiagen.com/se/resources/resourcedetail?id=c5fe7d5f-070a-4ebe-ac04-4bbf05a13e91&lang=en", "[DNA extraction] Int... |
49,077 | Does Perioperative Respiratory Event Increase Length of Hospital Stay and Hospital Cost in Pediatric Ambulatory Surgery? | 1 | dx.doi.org/10.17504/protocols.io.bt6vnre6 | https://www.protocols.io/view/does-perioperative-respiratory-event-increase-leng-bt6vnre6 | oomaliwa , oomaliwa | TITLE: Does Perioperative Respiratory Event Increase Length of Hospital Stay and Hospital Cost in Pediatric Ambulatory Surgery?
AUTHORS: oomaliwa , oomaliwa
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Objective</span><span>: We </span><a href="" style = "text-d... | ["[Objective and methodology]\nObjectiveTo examine if the occurrence of PRE is associated with prolonged the length of stay and increased hospitalcost in pediatric ambulatory surgery.", "[Objective and methodology]\nMethodsThis study was part of a larger research project (ClinicalTrials.gov: NCT02036021). Children aged... |
82,322 | Primary cortical astrocyte isolation and culture | 3 | dx.doi.org/10.17504/protocols.io.x54v9dqqmg3e/v1 | https://www.protocols.io/view/primary-cortical-astrocyte-isolation-and-culture-cumswu6e | Shiyi Wang | TITLE: Primary cortical astrocyte isolation and culture
AUTHORS: Shiyi Wang
[DESCRIPTION]
Primary cortical astrocyte isolation and culture
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.j9pcr5n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A 4-month-old male null mouse swimming. The water temperature was 34 °C.</p>
[STEPS]
?. | [] |
73,242 | Catalogación Monografías Biblioteca UCAM | 1 | dx.doi.org/10.17504/protocols.io.36wgqjn4kvk5/v1 | https://www.protocols.io/view/catalogaci-n-monograf-as-biblioteca-ucam-cjr2um8e | Antonio Rex Alegria | TITLE: Catalogación Monografías Biblioteca UCAM
AUTHORS: Antonio Rex Alegria
[DESCRIPTION]
El objetivo de la catalogación es la selección y descripción de los elementos informativos que permiten identificar un documento así como de establecer los puntos de acceso para su posterior recuperación.
[STEPS]
1. La adq... | ["La adquisición de fondos bibliográficos y documentales se realiza a través del soporte. La dirección de la biblioteca aprobará la petición y pasará al Servicio de Unidad de Compras que procederá a la petición al proveedor.\n\nCuando los documentos llegan a la Biblioteca, se sellan con el sello de registro en la porta... |
67,406 | Growth Matrix Male Enhancement (Zero Hassle) #1 Male Pill USA | 3 | dx.doi.org/10.17504/protocols.io.261genzpjg47/v1 | https://www.protocols.io/view/growth-matrix-male-enhancement-zero-hassle-1-male-cd3ns8me | alevinerames | TITLE: Growth Matrix Male Enhancement (Zero Hassle) #1 Male Pill USA
AUTHORS: alevinerames
[DESCRIPTION]
https://google-news.clubeo.com/page/growth-matrix-male-enhancement-zero-hassle-1-male-pill-usa.html
[STEPS] | [] |
20,153 | U Mass - Gamma-glutamyl Transferase | null | dx.doi.org/10.17504/protocols.io.xwzfpf6 | null | Jason Kim | TITLE: U Mass - Gamma-glutamyl Transferase
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex t... | ["Add 200 µL of Wash Buffer into each well of the plate. Seal and mix on a plate shaker for 10 minutes at room temperature (20-25°C).", "Decant Wash Buffer and remove the residual amount from all wells by inverting the plate and tapping it smartly onto absorbent towels several times.", "Add 25 µL of each Standard or C... |
108,719 | RT-qPCR | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qkzxl1y/v1 | https://www.protocols.io/view/rt-qpcr-dnep5bdn | Karyna Tarasova, Sinan Gültekin, Angkana Kidtiwong, iris.gerner Gerner, Florien Jenner | TITLE: RT-qPCR
AUTHORS: Karyna Tarasova, Sinan Gültekin, Angkana Kidtiwong, iris.gerner Gerner, Florien Jenner
[DESCRIPTION]
Gene expression levels by RT-qPCR
[STEPS]
SECTION: RT-qPCR
1. Phase separation
SECTION: RT-qPCR
1.1. For gene expression analysis adherent cells were lysed using a mixture of TRIzol reagent (In... | ["[RT-qPCR] Phase separation", "[RT-qPCR] For gene expression analysis adherent cells were lysed using a mixture of TRIzol reagent (Invitrogen, Thermo Scientific, Germany) + 1% β-Mercaptoethanol (Sigma-Aldrich, USA) (100µL/per well in 96 well plate), snap frozen and stored at -80°C until further processing. After thawi... |
43,619 | Protocol for intracellular Ca2+ detection using FlexStation 3 | 1 | dx.doi.org/10.17504/protocols.io.bnubmesn | https://www.protocols.io/view/protocol-for-intracellular-ca2-detection-using-fle-bnubmesn | Pengyun Li; Shang-Zhong Xu | TITLE: Protocol for intracellular Ca2+ detection using FlexStation 3
AUTHORS: Pengyun Li; Shang-Zhong Xu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for Ca2+ detection using FlexStation 3 . See attachment</div></div>
[STEPS]
?. | [] |
18,528 | Transformation Aspergillus niger using Cas9, AMA1 vector, pyrG rescue marker and sgRNA in vitro. | null | dx.doi.org/10.17504/protocols.io.wb8farw | null | Laure Leynaud-Kieffer | TITLE: Transformation Aspergillus niger using Cas9, AMA1 vector, pyrG rescue marker and sgRNA in vitro.
AUTHORS: Laure Leynaud-Kieffer
[STEPS]
?. [Ahead before the transformation]
-Prepare the construct containing the marker pyrG. We used the Phusion Hot Start II to amplify the plasmid containing the construct. The PC... | ["[Ahead before the transformation]\n-Prepare the construct containing the marker pyrG. We used the Phusion Hot Start II to amplify the plasmid containing the construct. The PCR product is purify and concentrate to 1ug/uL and we need 10ug for the transformation.-Prepare the plasmid containing the Cas9 cassette, the hyg... |
101,060 | Three-dimensional modeling of coral skeletal fragments for morphometrics | 0 | dx.doi.org/10.17504/protocols.io.yxmvmeep6g3p/v1 | https://www.protocols.io/view/three-dimensional-modeling-of-coral-skeletal-fragm-dexc3fiw | Colin J Anthony, Colin Lock, Renee Crisostomo, Bastian Bentlage | TITLE: Three-dimensional modeling of coral skeletal fragments for morphometrics
AUTHORS: Colin J Anthony, Colin Lock, Renee Crisostomo, Bastian Bentlage
[DESCRIPTION]
This protocol details a comprehensive method for scanning and modeling coral fragments to accurately calculate surface area, volume, and other key morph... | ["[Skeletal Preparation] Airbrush tissue from coral skeleton with filtered seawater until only a bare skeleton remains.", "[Skeletal Preparation] Place fragments in drying oven overnight (50°C) to evaporate remaining water from fragment.", "[Skeletal Preparation] Weigh dried fragments.\n\nThis will be used later to cal... |
50,924 | Full genome PCR amplification of all African Hepatitis B Virus genotypes | 4 | dx.doi.org/10.17504/protocols.io.bvykn7uw | https://www.protocols.io/view/full-genome-pcr-amplification-of-all-african-hepat-bvykn7uw | Louis S IV Le Clercq, Sheila Mary Bowyer, Simnikiwe Mayaphi | TITLE: Full genome PCR amplification of all African Hepatitis B Virus genotypes
AUTHORS: Louis S IV Le Clercq, Sheila Mary Bowyer, Simnikiwe Mayaphi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This method is and adaptation of the full genome amplification protocol published by Gunther </sp... | ["[Master Mix preparation]\nPrepare the following two mixes :\non ice", "[Master Mix preparation]\nMaster Mix 1:For one reaction combine the following:dNTPs25 mM200 uM0.2 uLP115 uM300 nM0.5 uLP2 or P2_A115 uM300 nM0.5 uLExpand Hi Fi Buffer10x1x2 uLMgCl215 mM1.5 mM-ddH2On.a.n.a.11.8 uL\n15 µl\ndNTPs25 mM200 uM0.2 uLP1... |
54,177 | Experiment 2 | 1 | null | https://www.protocols.io/view/experiment-2-by59py96 | Dikla Perez , Yael Steinhart , Amir Grinstein , Meike Morren | TITLE: Experiment 2
AUTHORS: Dikla Perez , Yael Steinhart , Amir Grinstein , Meike Morren
[DESCRIPTION]
In this experiment we aimed to achieve two goals. First, we aimed to replicate the effect of expected product visibility on consistency in sequential decisions, and to extend our conclusions to circumstances in whi... | ["Experimental design:\nWe have employed two expected visibility conditions (low vs. high) in a between-subjects design.", "Sample:\nn = 78 student participants (61.5% women, Mage = 22.6, SD = 2.05) volunteered to take part in an online study in exchange for course credit. Participants were each randomly assigned to on... |
80,868 | Cross-sectional study on electroencephalographic oscillations when achieving heart coherence in medical students at the Technological University of Pereira | 1 | dx.doi.org/10.17504/protocols.io.kqdg39zk7g25/v1 | https://www.protocols.io/view/cross-sectional-study-on-electroencephalographic-o-cs8cwhsw | Juan David Morales Rudas | TITLE: Cross-sectional study on electroencephalographic oscillations when achieving heart coherence in medical students at the Technological University of Pereira
AUTHORS: Juan David Morales Rudas
[DESCRIPTION]
Heart coherence is a technique that consists of synchronizing breathing with heart rate, which produces phys... | ["[SUMMARY] SUMMARY\n\nHeart coherence is a technique that consists of synchronizing breathing with heart rate, which produces physiological and psychological benefits. The aim of this cross-sectional study is to analyze the characteristics of electroencephalographic oscillations during the implementation of the cardia... |
null | null | null | dx.doi.org/10.17504/protocols.io.iamcac6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This single-tube library construction protocol is for degraded DNA using BGISeq-500 sequencing platform.</p>
[BEFORE_START]
<p>Calculate the DNA input and prepare the library adapters.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
88,347 | BeBOP Protocol Templates | 2 | null | https://www.protocols.io/view/bebop-protocol-templates-c2h3yb8n | Kathleen Pitz, Raissa.meyer | TITLE: BeBOP Protocol Templates
AUTHORS: Kathleen Pitz, Raissa.meyer
[DESCRIPTION]
A collection of protocol templates created through the BeBOP project.
[STEPS] | [] |
48,076 | Chromosomal DNA extraction from Gram-positive bacteria | 4 | dx.doi.org/10.17504/protocols.io.bs7knhkw | https://www.protocols.io/view/chromosomal-dna-extraction-from-gram-positive-bact-bs7knhkw | Anders Kiledal, Julia Maresca | TITLE: Chromosomal DNA extraction from Gram-positive bacteria
AUTHORS: Anders Kiledal, Julia Maresca
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Extraction of high-molecular-weight DNA from Gram-positive bacterial species, with optional steps for removing surfactants. This DNA is suitable ... | ["[Grow culture]\nInoculate 10 mL rich medium from a fresh overnight culture, and incubate at appropriate temperature on shaker. At OD600 of ~0.8-1.0, harvest cells by centrifugation (10 min., 5000 rpm).", "[Cell lysis]\nResuspend cell pellet in 2 mL TEN (10 mM Tris-HCl, pH 8.0, 10 mM EDTA, 150 mM NaCl).", "[Cell lysis... |
null | null | null | dx.doi.org/10.17504/protocols.io.crtv6m | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the protocol for making two 250 ml cultures of electrocompetent cells
[BEFORE_START]
Sterile 10% glycerol (can be autoclaved) is needed for the washes. The volume of 10% glycerol needed is 2X the culture volume (for example, a 500 ml culture requires 1L of 10%... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.prjdm4n | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<p>Media preparation:</p>
<p>1M KH<sub>2</sub>PO<sub>4</sub> 100ml → 13,61g</p>
<p>1M K<sub>2</sub>HPO<sub>4</sub> 100ml → 17,42g</p>
<p>1M Na-Acetate 100ml → 13,61g Trihydrate</p>
<p>1M Acetic acid 100ml → 60,05g (liquid)</p>
<p>1M Glycine 100ml → 7,51g</p>
<p> </p>
<p>Autocla... | [] |
97,593 | Aerobic Intradialytic Exercise | 0 | dx.doi.org/10.17504/protocols.io.ewov19yz2lr2/v1 | https://www.protocols.io/view/aerobic-intradialytic-exercise-dbiz2kf6 | Siti Nur Rohmah, Andika Dhamarjati, Metalia Puspitasari | TITLE: Aerobic Intradialytic Exercise
AUTHORS: Siti Nur Rohmah, Andika Dhamarjati, Metalia Puspitasari
[DESCRIPTION]
Currently, intradialytic exercise interventions are rarely performed in hemodialysis centres in Indonesia, particularly at Dr Sardjito Hospital. Physical exercise during hemodialysis (HD) provides many ... | ["The subject wears comfortable clothes that do not interfere with the movement of the feet and brings thick socks", "Measure the patient's blood pressure, pulse, and saturation before the exercise.", "The subject is given information on how to use the cycle ergometer and how to determine the intensity of exercise usin... |
70,740 | jres_c13.nan | 5 | dx.doi.org/10.17504/protocols.io.5qpvor567v4o/v1 | https://www.protocols.io/view/jres-c13-nan-chbut2nw | NAN-KB UGA | TITLE: jres_c13.nan
AUTHORS: NAN-KB UGA
[DESCRIPTION]
This is a protocol for running a 2D carbon-carbon homonuclear JRES pulseprogram 'jresdcqfsp.pp'.
It will provide a dataset where F2 gives the carbon 1D signals according to chemical shift and the indirect F1 dimension shows the carbon-carbon scalar coupling patte... | ["[Create JRES_C13 experiment file] Starting from either the 1D proton or carbon data sets, click on Acquire -> 'Create Dataset' button to open dataset entry box.\nIt should increment the EXPNO to the next integer ( e.g. 3)", "[Create JRES_C13 experiment file] Click OK at bottom of window to create the experiment di... |
30,609 | Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA) | null | dx.doi.org/10.17504/protocols.io.95rh856 | null | Sam Li | TITLE: Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA)
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. Clear Slides: Remove paraffin and hydrate the tissue.Note: If using frozen sections, allow slides to come to room temperature for 15 minutes & proceed to step (F)... | ["Clear Slides: Remove paraffin and hydrate the tissue.Note: If using frozen sections, allow slides to come to room temperature for 15 minutes & proceed to step (F) onlyXylene - 5 minutes in each of (3) different 250 mL containers100% alcohol - 5 minutes in each of (3) different 250 mL containers95% alcohol - 3 minutes... |
35,747 | PCR with Q5® Polymerase (M0491) | 1 | dx.doi.org/10.17504/protocols.io.be6bjhan | https://www.protocols.io/view/pcr-with-q5-polymerase-m0491-be6bjhan | New England Biolabs | TITLE: PCR with Q5® Polymerase (M0491)
AUTHORS: New England Biolabs
[DESCRIPTION]
This protocol describes methods for PCR using Q5® High-Fidelity DNA Polymerase (M0491).
[BEFORE_START]
Please note that protocols with Q5® High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions reco... | ["Set up the following reaction on ice:", "Gently mix the reaction.", "Collect all liquid to the bottom of the tube by a quick spin if necessary and overlay the sample with mineral oil if using a PCR machine without a heated lid.", "Quickly transfer PCR tubes to a PCR machine preheated to the denaturation temperature (... |
19,076 | Determination of the total acid number (TAN) in crude oils | null | dx.doi.org/10.17504/protocols.io.wvcfe2w | null | Jonas Sundberg | TITLE: Determination of the total acid number (TAN) in crude oils
AUTHORS: Jonas Sundberg
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Description</span></div><div class = "text-block">Titrimetric determination of total acid number (TAN) in crude oil. Protocol is... | ["[Setup]\nAssemble the titrator inside a ventilated fume-hood as samples are stored in open containers. Keyboard is optional, but highly recommended for entering of sample details. To store results as a PDF document, insert a USB memory stick (without encryption) into a USB-port in the back of the titrator. To avoid d... |
89,237 | Collections Standard Operating Protocol, Organism group: Lichens | 1 | dx.doi.org/10.17504/protocols.io.dm6gp3y7jvzp/v1 | https://www.protocols.io/view/collections-standard-operating-protocol-organism-g-c3dvyi66 | Amanda L Jones, Laura L Forrest, Michelle Hart, David Bell, Rebecca Yahr | TITLE: Collections Standard Operating Protocol, Organism group: Lichens
AUTHORS: Amanda L Jones, Laura L Forrest, Michelle Hart, David Bell, Rebecca Yahr
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedure (SOP) for the Plant Working Group". The SOP collection contains guidan... | [] |
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