Datasets:
jackkuo
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LLM-Ribozyme-Kinetics-Golden-Benchmark

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1
Ribozyme
stringlengths
2
37
Organism
stringclasses
56 values
Variable_reactant
stringlengths
2
100
⌀
Kobs
stringlengths
1
19
⌀
Unit_Kobs
stringclasses
11 values
km
stringclasses
287 values
Unit_Km
stringclasses
11 values
kcat
stringclasses
262 values
Unit_Kcat
stringclasses
9 values
km_kcat
stringlengths
1
16
⌀
Unit_Kcat/Km
stringclasses
30 values
Kcleav
stringclasses
52 values
Unit_Kcleav
stringclasses
3 values
Commentary[Temp]
stringclasses
29 values
Commentary[pH]
stringclasses
34 values
Commentary[Mutant]
stringlengths
2
89
⌀
Commentary[Others]
stringlengths
3
94
⌀
pubmed_id
stringclasses
164 values
Source
stringclasses
4 values
Hairpin
Tobacco ringspot virus
O^6Me-G at SG+1
NA
NA
NA
NA
<0.0002
min^-1
NA
1
NA
NA
37°C
7.5
SG+1 to O^6Me-G
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cG at RzA G8
NA
NA
0.08
µM
0.0026
min^-1
0.012
1
NA
NA
37°C
7.5
RzA G8 to ^7cG
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
I at RzA G8
NA
NA
NA
NA
0.0043
min^-1
0.023
1
NA
NA
37°C
7.5
RzA G8 to I
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
O^6Me-G at RzA G8
NA
NA
NA
NA
0.0028
min^-1
0.016
1
NA
NA
37°C
7.5
RzA G8 to O^6Me-G
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cG at RzA A9
NA
NA
0.44
µM
0.015
min^-1
0.012
1
NA
NA
37°C
7.5
RzA A9 to ^7cG
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzA A9
NA
NA
0.11
µM
0.026
min^-1
0.086
1
NA
NA
37°C
7.5
RzA A9 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzA A9
NA
NA
0.1
µM
0.01
min^-1
0.036
1
NA
NA
37°C
7.5
RzA A9 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzA A10
NA
NA
0.22
µM
0.013
min^-1
0.021
1
NA
NA
37°C
7.5
RzA A10 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzA A10
NA
NA
0.22
µM
0.027
min^-1
0.045
1
NA
NA
37°C
7.5
RzA A10 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
I at RzA G11
NA
NA
0.08
µM
0.015
min^-1
0.066
1
NA
NA
37°C
7.5
RzA G11 to I
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
O^6Me-G at RzA G11
NA
NA
0.08
µM
0.0045
min^-1
0.021
1
NA
NA
37°C
7.5
RzA G11 to O^6Me-G
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cG at RzA G11
NA
NA
0.21
µM
0.017
min^-1
0.03
1
NA
NA
37°C
7.5
RzA G11 to ^7cG
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
I at RzA G21
NA
NA
0.09
µM
0.0029
min^-1
0.012
1
NA
NA
37°C
7.5
RzA G21 to I
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
O^6Me-G at RzA G21
NA
NA
0.08
µM
0.0025
min^-1
0.012
1
NA
NA
37°C
7.5
RzA G21 to O^6Me-G
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cG at RzA G21
NA
NA
0.46
µM
0.021
min^-1
0.017
1
NA
NA
37°C
7.5
RzA G21 to ^7cG
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzA A22
NA
NA
0.13
µM
0.026
min^-1
0.08
1
NA
NA
37°C
7.5
RzA A22 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzA A22
NA
NA
NA
NA
0.0004
min^-1
0.002
1
NA
NA
37°C
7.5
RzA A22 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzA A23
NA
NA
0.08
µM
0.018
min^-1
0.087
1
NA
NA
37°C
7.5
RzA A23 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzA A23
NA
NA
0.14
µM
0.026
min^-1
0.106
1
NA
NA
37°C
7.5
RzA A23 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzA A24
NA
NA
NA
NA
0.0021
min^-1
0.012
1
NA
NA
37°C
7.5
RzA A24 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzA A24
NA
NA
0.09
µM
0.22
min^-1
0.9
1
NA
NA
37°C
7.5
RzA A24 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzB A38
NA
NA
NA
NA
0.0007
min^-1
0.004
1
NA
NA
37°C
7.5
RzB A38 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzB A38
NA
NA
0.11
µM
0.013
min^-1
0.046
1
NA
NA
37°C
7.5
RzB A38 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzB A40
NA
NA
0.03
µM
0.047
min^-1
0.66
1
NA
NA
37°C
7.5
RzB A40 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzB A40
NA
NA
0.17
µM
0.26
min^-1
0.55
1
NA
NA
37°C
7.5
RzB A40 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
P at RzB A43
NA
NA
0.05
µM
0.062
min^-1
0.47
1
NA
NA
37°C
7.5
RzB A43 to P
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Hairpin
Tobacco ringspot virus
^7cA at RzB A43
NA
NA
0.22
µM
0.0012
min^-1
0.002
1
NA
NA
37°C
7.5
RzB A43 to ^7cA
10 mM MgCl2, 40 mM Tris·HCl
7535099
Table
Class I polynucleotide kinase
NA
5'-GGAACCU-3' , ATP-γS , A
NA
NA
3.1
mM
0.17
min^-1
57
M^-1 min^-1
NA
NA
30°C
7
WT
50 mM MgCl2, 5 mM MnCl2, 400 mM KCl
7578148
Table
Class I polynucleotide kinase
NA
5'-GGAACCU-3', ATP , A
NA
NA
3.4
mM
0.003
min^-1
0.9
M^-1 min^-1
NA
NA
30°C
7
WT
50 mM MgCl2, 5 mM MnCl2, 400 mM KCl
7578148
Table
Class I polynucleotide kinase
NA
5'-GGAACCU-3' , ATP-γS , rS
NA
NA
2
µM
0.17
min^-1
8.5 × 10^4
M^-1 min^-1
NA
NA
30°C
7
WT
50 mM MgCl2, 5 mM MnCl2, 400 mM KCl
7578148
Table
Class I polynucleotide kinase
NA
5'-GGAACCU-3' , ATP , rS
NA
NA
2
µM
0.003
min^-1
0.14 × 10^4
M^-1 min^-1
NA
NA
30°C
7
WT
50 mM MgCl2, 5 mM MnCl2, 400 mM KCl
7578148
Table
Class I polynucleotide kinase
NA
5'-HO-GGAACC-3'
NA
NA
25
µM
0.05
min^-1
NA
NA
NA
NA
30°C
7
WT
50 mM MgCl2, 5 mM MnCl2, 400 mM KCl
7578148
Text
Class I polynucleotide kinase
NA
5'-HO-d(GGAACCT)-3'
0.003
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
30°C
7
WT
50 mM MgCl2, 5 mM MnCl2, 400 mM KCl
7578148
Text
Class I ligase
NA
Substrate complex
NA
NA
9
µM
100
min^-1
NA
NA
NA
NA
22°C
NA
b1-210t
NA
7618102
Table
Class II ligase
NA
Substrate complex
1.1 × 10^-2
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
NA
a4-10t
NA
7618102
Table
Class II ligase
NA
Substrate complex
5 × 10^-3
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
37°C
NA
c2-10t
NA
7618102
Table
Class II ligase
NA
Substrate complex
1.5 × 10^-2
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
NA
d1-10t
NA
7618102
Table
Class II ligase
NA
Substrate complex
4 × 10^-4
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
37°C
NA
f1-10t
NA
7618102
Table
Class III ligase
NA
Substrate complex
3 × 10^-6
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
30°C
NA
e3-10t
NA
7618102
Table
Class III ligase
NA
Substrate complex
1.5 × 10^-5
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
37°C
NA
g1-10t
NA
7618102
Table
Group I ribozyme
Tetrahymena
DNA substrate, 5'- TAA(TAAA)3-3'
NA
NA
NA
NA
1 × 10^-6
min^-1
NA
NA
NA
NA
37°C
7.5
Evolved ribozyme (generation 27, clone 48)
10 mM MgCl2, 30 mM EPPS
7809628
Text
Group I ribozyme
Tetrahymena
DNA substrate, 5'-TAG(TAAA)3-3'
NA
NA
NA
NA
1 × 10^-5
min^-1
NA
NA
NA
NA
37°C
7.5
Evolved ribozyme (generation 27, clone 48)
10 mM MgCl2, 30 mM EPPS
7809628
Text
Group I ribozyme
Tetrahymena
DNA substrate
NA
NA
NA
NA
NA
NA
40
M^-1 min^-1
NA
NA
NA
NA
WT
NA
7809628
Text
Group I ribozyme
Tetrahymena
RNA substrate
NA
NA
NA
NA
NA
NA
1 × 10^7
M^-1 min^-1
NA
NA
NA
NA
WT
NA
7809628
Text
Group I ribozyme
Tetrahymena
DNA substrate with arabinonucleoside
NA
NA
NA
NA
NA
NA
3 × 10^7
M^-1 min^-1
NA
NA
NA
NA
Evolved ribozyme
NA
7809628
Text
HIV-1 pol specific hairpin ribozyme
Human immunodeficiency virus 1
Substrate
NA
NA
6.7
nM
0.5
min^-1
75
min^-1 µM^-1
NA
NA
37°C
7.5
Tetraloop modification
12 mM MgCl2, 2 mM spermidine, 40 mM Tris
7831794
Table
HIV-1 pol hairpin ribozyme
Human immunodeficiency virus 1
Substrate
NA
NA
42
nM
0.2
min^-1
5
min^-1 µM^-1
NA
NA
37°C
7.5
Tetraloop modification
12 mM MgCl2, 2 mM spermidine, 40 mM Tris
7831794
Table
HIV-1 5' leader hairpin ribozyme
Human immunodeficiency virus 1
Substrate
NA
NA
100
nM
1.6
min^-1
16
min^-1 µM^-1
NA
NA
37°C
7.5
Tetraloop modification
12 mM MgCl2, 2 mM spermidine, 40 mM Tris
7831794
Table
Native hairpin ribozyme
NA
UGACA*GUCCUGUUU
NA
NA
30
nM
2.1
min^-1
70
min^-1 µM^-1
NA
NA
37°C
7.5
Tetraloop modification
12 mM MgCl2, 2 mM spermidine, 40 mM Tris
7831794
Table
VS ribozyme
Neurospora
S
NA
NA
0.13
µM
0.7
min^-1
NA
NA
NA
NA
30°C
8
WT
50 mM Tris-HCl, 5 mM MgCl2, 2 mM spermidine, 25 mM KCl
7835347
Text
VS ribozyme
Neurospora
S
NA
NA
NA
NA
NA
NA
NA
NA
0.6
min^-1
30°C
7.1
WT
50 mM Tris-HCl, 25 mM MgCl2, 25 mM KCl, 2 mM spermidine
7835347
Text
Group II ribozyme
Saccharomyces cerevisiae
E1a -17/7
NA
NA
6.3
nM
0.021
min^-1
3.3 × 10^6
M^-1 min^-1
NA
NA
42°C
7.5
WT
D5 saturating (3 µM)
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
E1a -17/8
NA
NA
9.2
nM
0.02
min^-1
2.2 × 10^6
M^-1 min^-1
NA
NA
42°C
7.5
WT
multiple-turnove
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
E1a -17/9
NA
NA
870
nM
0.019
min^-1
2.2 × 10^4
M^-1 min^-1
NA
NA
42°C
7.5
WT
D1 saturating (25 nM)
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
E1a -19/8
NA
NA
NA
NA
0.032
min^-1
NA
NA
NA
NA
42°C
7.5
WT
D1 and D5 saturating
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
E1a -19/8 (phosphorothioate)
0.0094
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
WT
NA
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
E1a -17/7 (phosphorothioate)
0.012
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
WT
NA
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
5'-GGUGUGGUGGGACAUUUUC-GAGCGGUU
0.036
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
WT
NA
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
5'-GGUGUGGUGGGACAUUUUC-AAGCGGUU
0.053
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
+1A mutation
NA
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
5'-GGUGUGGUGGGACAUUUUC-UAGCGGUU
0.028
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
+1U mutation
NA
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
5'-GGUGUGGUGGGACAUUUUC-CAGCGGUU
0.0019
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
+1C mutation
NA
7893710
Table
Group II ribozyme
Saccharomyces cerevisiae
5'GGAGUGGUGGGACAUUUUCACUAUGUA
0.0068
min^-1
NA
NA
NA
NA
NA
NA
NA
NA
42°C
7.5
WT
NA
7893710
Table
Group II intron
NA
D5
NA
NA
270
nM
null
null
1.5 × 10^5
M^-1min^-1
NA
NA
45°C
7.5
WT
100 mM MgCl2, 500 mM KCl
8117737
Table
Group II intron
null
exD123
NA
NA
190
nM
null
null
5.5 × 10^5
M^-1min^-1
NA
NA
45°C
7.5
WT
100 mM MgCl2, 500 mM KCl
8117737
Table
Hammerhead
NA
NA
NA
NA
74
nM
1.51
min^-1
20.4
µM^-1 min^-1
NA
NA
25°C
7.5
WT
10 mM MgCl2
8233777
Table
Hammerhead
NA
NA
NA
NA
136
nM
0.021
min^-1
0.16
µM^-1 min^-1
NA
NA
25°C
7.5
O^6MeG5
10 mM MgCl2
8233777
Table
Hammerhead
NA
NA
NA
NA
113
nM
0.02
min^-1
0.18
µM^-1 min^-1
NA
NA
25°C
7.5
O^6MeG8
10 mM MgCl2
8233777
Table
Hammerhead
NA
NA
NA
NA
81
nM
0.025
min^-1
0.31
µM^-1 min^-1
NA
NA
25°C
7.5
O^6MeG12
10 mM MgCl2
8233777
Table
R32
NA
NA
0.02
µM
4
min^-1
200
µM^-1min^-1
NA
NA
NA
NA
37°C
8
WT
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
DRD32
NA
NA
1.3
µM
13
min^-1
10
µM^-1min^-1
NA
NA
NA
NA
37°C
8
DNA in stems I and III
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
DRDRD32
NA
NA
1.2
µM
16
min^-1
13
µM^-1min^-1
NA
NA
NA
NA
37°C
8
DNA in stems I, II, and III
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
thio-DRDRD32
NA
NA
4.4
µM
27
min^-1
6.1
µM^-1min^-1
NA
NA
NA
NA
37°C
8
Phosphorothioate linkages in DNA domains
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
thio-DRDRD32-3S
NA
NA
3.4
µM
6.7
min^-1
2
µM^-1min^-1
NA
NA
NA
NA
37°C
8
Three phosphorothioate linkages at C3, U4, and U7
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
thio-DRDRD32-2SA
NA
NA
0.41
µM
5.5
min^-1
13
µM^-1min^-1
NA
NA
NA
NA
37°C
8
Two phosphorothioate linkages at C3 and U4, U7 replaced by A7
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
thio-DRDRD32-2SG
NA
NA
0.43
µM
2
min^-1
4.7
µM^-1min^-1
NA
NA
NA
NA
37°C
8
Two phosphorothioate linkages at C3 and U4, U7 replaced by G7
25 mM MgCl2, 50 mM Tris-HCl
8332458
Table
Rz1
NA
Substrate
NA
NA
87
nM
3.1
min^-1
36
µM^-1·min^-1
NA
NA
25°C
7.5
WT
10 mM MgCl2
8346207
Table
Rz2
NA
Substrate
NA
NA
80
nM
0.012
min^-1
0.16
µM^-1·min^-1
NA
NA
25°C
7.5
G^10.1C^11.1 to C^10.1G^11.1
10 mM MgCl2
8346207
Table
Rz3
NA
Substrate
NA
NA
140
nM
4.7
min^-1
33
µM^-1·min^-1
NA
NA
25°C
7.5
Shortened stem II, C(GCAA)G loop
10 mM MgCl2
8346207
Table
Rz4
NA
Substrate
NA
NA
115
nM
1.4
min^-1
12
µM^-1·min^-1
NA
NA
25°C
7.5
Shortened stem II, C(UUCG)G loop
10 mM MgCl2
8346207
Table
Rz5
NA
Substrate
NA
NA
200
nM
2.6
min^-1
13
µM^-1·min^-1
NA
NA
25°C
7.5
Shortened stem II, C(UUUU)G loop
10 mM MgCl2
8346207
Table
Rz6
NA
Substrate
NA
NA
350
nM
0.3
min^-1
0.86
µM^-1·min^-1
NA
NA
25°C
7.5
1 bp stem II, G(CUUG)C loop
10 mM MgCl2
8346207
Table
Rz7
NA
Substrate
NA
NA
240
nM
0.038
min^-1
0.16
µM^-1·min^-1
NA
NA
25°C
7.5
No stem II, d(TTTT) loop
10 mM MgCl2
8346207
Table
APPrbz-141s
Homo sapiens
βAPP-141
NA
NA
108
nM
0.13
min^-1
72
(µM-hr)^-1
NA
NA
37°C
NA
WT
10 mM MgCl2
8371986
Table
APPrbz-141l
Homo sapiens
βAPP-141
NA
NA
NA
NA
NA
NA
60
(µM-hr)^-1
NA
NA
37°C
NA
WT
10 mM MgCl2
8371986
Table
APPrbz-141l
Homo sapiens
βAPP-751
NA
NA
NA
NA
NA
NA
4
(µM-hr)^-1
NA
NA
37°C
NA
WT
10 mM MgCl2
8371986
Table
Hammerhead
NA
native
NA
NA
1.4
µM
0.69
min^-1
500 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
WT
10 mM MgCl2
8399208
Table
Hammerhead
NA
native
NA
NA
3.1
µM
0.19
min^-1
61 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
G5c^7G
10 mM MgCl2
8399208
Table
Hammerhead
NA
native
NA
NA
1.1
µM
0.096
min^-1
86 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
G8c^7G
10 mM MgCl2
8399208
Table
Hammerhead
NA
G12c^7G
NA
NA
1.2
µM
0.47
min^-1
380 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
WT
10 mM MgCl2
8399208
Table
Hammerhead
NA
G12I
NA
NA
11
µM
0.012
min^-1
1.1 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
WT
10 mM MgCl2
8399208
Table
Hammerhead
NA
A13P
NA
NA
1.9
µM
0.039
min^-1
20 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
WT
10 mM MgCl2
8399208
Table
Hammerhead
NA
A14P
NA
NA
1.7
µM
0.056
min^-1
33 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
WT
10 mM MgCl2
8399208
Table
Hammerhead
NA
A15P
NA
NA
1.8
µM
0.015
min^-1
8.3 × 10^-3
µM^-1·min^-1
NA
NA
55°C
8
WT
10 mM MgCl2
8399208
Table
RNase P
Escherichia coli
pre-tRNA^Asp
NA
NA
130
nM
1.7
min^-1
NA
NA
NA
NA
37°C
8
WT
Na^+, Mg^2+
8499432
Table
RNase P
Escherichia coli
pre-tRNA^Asp
NA
NA
21
nM
0.052
min^-1
NA
NA
NA
NA
37°C
8
WT
NH4^+, Ca^2+
8499432
Table
RNase P
Escherichia coli
pre-tRNA^Phe
NA
NA
48
nM
0.4
min^-1
NA
NA
NA
NA
37°C
8
WT
NH4^+, Mg^2+
8499432
Table
RNase P
Escherichia coli
rA-tRNA^Phe
NA
NA
62
nM
0.15
min^-1
NA
NA
NA
NA
37°C
8
WT
NH4^+, Mg^2+
8499432
Table
RNase P
Escherichia coli
dA-tRNA^Phe
NA
NA
30
nM
5.3 × 10^-3
min^-1
NA
NA
NA
NA
37°C
8
WT
NH4^+, Mg^2+
8499432
Table
RNase P
Escherichia coli
dA-tRNA^Phe
NA
NA
180
nM
0.074
min^-1
NA
NA
NA
NA
37°C
8
WT
Na^+, Mg^2+
8499432
Table
RNase P
Escherichia coli
rAm-tRNA^Phe
NA
NA
NA
NA
7 × 10^-5
min^-1
NA
NA
NA
NA
37°C
8
WT
NH4^+, Mg^2+
8499432
Table