Title
stringlengths 1
395
⌀ | abstractText
stringlengths 57
5.98k
| meshMajor
stringlengths 14
1.03k
| pmid
int64 22
33.2M
| meshid
stringlengths 2
3.14k
| meshroot
stringlengths 2
421
| A
int64 0
1
| B
int64 0
1
| C
int64 0
1
| D
int64 0
1
| E
int64 0
1
| F
int64 0
1
| G
int64 0
1
| H
int64 0
1
| I
int64 0
1
| J
int64 0
1
| L
int64 0
1
| M
int64 0
1
| N
int64 0
1
| Z
int64 0
1
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Structural and kinetic basis for the regulation and potentiation of Hsp104 function.
|
Hsp104 provides a valuable model for the many essential proteostatic functions performed by the AAA+ superfamily of protein molecular machines. We developed and used a powerful hydrogen exchange mass spectrometry (HX MS) analysis that can provide positionally resolved information on structure, dynamics, and energetics of the Hsp104 molecular machinery, even during functional cycling. HX MS reveals that the ATPase cycle is rate-limited by ADP release from nucleotide-binding domain 1 (NBD1). The middle domain (MD) serves to regulate Hsp104 activity by slowing ADP release. Mutational potentiation accelerates ADP release, thereby increasing ATPase activity. It reduces time in the open state, thereby decreasing substrate protein loss. During active cycling, Hsp104 transits repeatedly between whole hexamer closed and open states. Under diverse conditions, the shift of open/closed balance can lead to premature substrate loss, normal processing, or the generation of a strong pulling force. HX MS exposes the mechanisms of these functions at near-residue resolution.
|
['Adenosine Triphosphate', 'Amino Acid Substitution', 'Gene Expression Regulation, Fungal', 'Genetic Variation', 'Heat-Shock Proteins', 'Mutation', 'Protein Binding', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae Proteins']
| 32,277,033
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['E05.393.420.601.035', 'G05.558.109'], ['G05.308.330'], ['G05.365'], ['D12.776.580.216'], ['G05.365.590'], ['G02.111.679', 'G03.808'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.354.750']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Galectin-8 regulates targeting of Gp135/podocalyxin and lumen formation at the apical surface of renal epithelial cells.
|
Establishment of apical-basal polarity, through correct targeting of polarity determinants to distinct domains of the plasma membrane, is a fundamental process for the development of functioning epithelial tubules. Here we report that galectin (Gal)-8 regulates apical-basal polarity of Madin-Darby canine kidney (MDCK) cells via apical targeting of 135-kDa glycoprotein (Gp135). Gal-8 interacts with newly synthesized Gp135 in a glycan-dependent manner. Gal-8 knockdown induces aberrant lumens at the lateral domain and mistargeting of Gp135 to this structure, thus disrupting the kidney epithelial polarity of MDCK cells, which organize lumens at the apical surface. The O-glycosylation deletion mutant of Gp135 phenocopies the effect of Gal-8 knockdown, which suggests that Gal-8 is the decoding machinery for the apical sorting signals of Gp135 residing at its O-glycosylation-rich region. Collectively, our results reveal a new role of Gal-8 in the development of luminal organs by regulating targeting of apical polarity protein Gp135.-Lim, H., Yu, C.-Y., Jou, T.-S. Galectin-8 regulates targeting of Gp135/podocalyxin and lumen formation at the apical surface of renal epithelial cells.
|
['Animals', 'Cell Polarity', 'Dogs', 'Epithelial Cells', 'Galectins', 'Kidney', 'Madin Darby Canine Kidney Cells', 'Sialoglycoproteins']
| 28,747,404
|
[['B01.050'], ['G04.250'], ['B01.050.150.900.649.313.750.250.216.200'], ['A11.436'], ['D12.776.503.307'], ['A05.810.453'], ['A11.251.210.827', 'A11.436.589'], ['D12.644.233.800', 'D12.776.395.700']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Reconsolidation in a human fear conditioning study: a test of extinction as updating mechanism.
|
Disrupting reconsolidation seems to be a promising approach to dampen the expression of fear memory. Recently, we demonstrated that disrupting reconsolidation by a pharmacological manipulation specifically targeted the emotional expression of memory (i.e., startle response). Here we test in a human differential fear-conditioning paradigm with fear-relevant stimuli whether the spacing of a single unreinforced retrieval trial relative to extinction learning allows for "rewriting" the original fear association, thereby preventing the return of fear. In contrast to previous findings reported by Schiller et al. (2010), who used a single-method for indexing fear (skin conductance response) and fear-irrelevant stimuli, we found that extinction learning within the reconsolidation window did not prevent the recovery of fear on multiple indices of conditioned responding (startle response, skin conductance response and US-expectancy). These conflicting results ask for further critical testing given the potential impact on the field of emotional memory and its application to clinical practice.
|
['Adolescent', 'Adult', 'Analysis of Variance', 'Conditioning, Classical', 'Electromyography', 'Extinction, Psychological', 'Fear', 'Female', 'Galvanic Skin Response', 'Humans', 'Male', 'Mental Recall', 'Psychiatric Status Rating Scales', 'Reflex, Startle', 'Surveys and Questionnaires', 'Young Adult']
| 21,986,472
|
[['M01.060.057'], ['M01.060.116'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['F02.463.425.179.308'], ['E01.370.405.255', 'E01.370.530.255'], ['F02.463.425.770.232'], ['F01.470.361'], ['E05.796.332', 'F02.830.702.315', 'F04.669.332', 'G07.265.563', 'G13.750.415'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.425.540.641'], ['F04.711.513.653'], ['E01.370.376.550.650.800', 'E01.370.600.550.650.800', 'F02.830.702.807', 'G11.561.731.869'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The role of the fat body, midgut and ovary in vitellogenin production and vitellogenesis in the female tick, Dermacentor variabilis.
|
Polyclonal antibodies directed against D. variabilis vitellin were utilized for immunocytochemistry at the ultrastructural level. We localized vitellogenin (Vg) in rough endoplasmic reticulum cisternae, secretory granules and secreted products of fat body trophocytes and midgut vitellogenic cells from feeding and ovipositing females. Vg was localized in the oocyte Golgi bodies and in the yolk bodies of both feeding and ovipositing females. Uptake of exogenous Vg was indicated by the presence of immunospecific gold probe in coated pits and coated vesicles at the apical plasma membrane of oocytes from females in rapid engorgement and oviposition. In unmated females little detectable evidence of Vg uptake by developing oocytes suggests that mating and host detachment signal the beginning of vitellogenesis. We conclude that fat body trophocytes, midgut vitellogenic cells and oocytes are involved in the synthesis and/or processing of Vg and that feeding is the signal associated with the initiation of Vg synthesis and/or processing.
|
['Animals', 'Dermacentor', 'Fat Body', 'Female', 'Immunohistochemistry', 'Microscopy, Electron', 'Ovary', 'Vitellogenesis', 'Vitellogenins']
| 1,639,570
|
[['B01.050'], ['B01.050.500.131.166.132.832.400.200'], ['A13.365'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['E01.370.350.515.402', 'E05.595.402'], ['A05.360.319.114.630', 'A05.360.576.497', 'A06.300.312.497'], ['G04.152.650.249.880', 'G08.686.784.310.500.880'], ['D12.776.093.500.925', 'D12.776.290.812.500', 'D12.776.744.925']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Interaction of vanadate with the cloned beta cell K(ATP) channel.
|
Vanadate is used as a tool to trap magnesium nucleotides in the catalytic site of ATPases. However, it has also been reported to activate ATP-sensitive potassium (K(ATP)) channels in the absence of nucleotides. K(ATP) channels comprise Kir6.2 and sulfonylurea receptor subunits (SUR1 in pancreatic beta cells, SUR2A in cardiac and skeletal muscle, and SUR2B in smooth muscle). We explored the effect of vanadate (2 mM), in the absence and presence of magnesium nucleotides, on different types of cloned K(ATP) channels expressed in Xenopus oocytes. Currents were recorded from inside-out patches. Vanadate inhibited Kir6.2/SUR1 currents by approximately 50% but rapidly activated Kir6.2/SUR2A ( approximately 4-fold) and Kir6. 2/SUR2B ( approximately 2-fold) currents. Mutations in SUR that abolish channel activation by magnesium nucleotides did not prevent the effects of vanadate. Studies with chimeric SUR indicate that the first six transmembrane domains account for the difference in both the kinetics and the vanadate response of Kir6.2/SUR1 and Kir6. 2/SUR2A. Boiling the vanadate solution, which removes the decavanadate polymers, largely abolished both stimulatory and inhibitory actions of vanadate. Our results demonstrate that decavanadate modulates K(ATP) channel activity via the SUR subunit, that this modulation varies with the type of SUR, that it differs from that produced by magnesium nucleotides, and that it involves transmembrane domains 1-6 of SUR.
|
['Adenosine Triphosphate', 'Animals', 'Ion Channel Gating', 'Islets of Langerhans', 'Patch-Clamp Techniques', 'Potassium Channels', 'Vanadates', 'Xenopus laevis']
| 10,464,267
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['B01.050'], ['G02.111.820.400', 'G04.835.400', 'G07.265.625'], ['A03.734.414', 'A06.300.414'], ['E05.200.500.905', 'E05.242.800'], ['D12.776.157.530.400.600', 'D12.776.543.550.450.750', 'D12.776.543.585.400.750'], ['D01.248.497.158.952', 'D01.960.960'], ['B01.050.150.900.090.180.610.500.562']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Two new species of Acroceratitis Hendel (Diptera: Tephritidae) and an updated key for the species from India.
|
Two new species of genus Acroceratitis Hendel, namely A. parastriata David & Hancock , sp. nov. and A. breviscapa David, Ramani & Hancock, sp. nov., are described from India. A. histrionica (de Meijere) is recorded for the first time from India. An updated key to Indian species of Acroceratitis is also provided.
|
['Animal Structures', 'Animals', 'Body Size', 'Female', 'India', 'Male', 'Organ Size', 'Tephritidae']
| 25,543,577
|
[['A13'], ['B01.050'], ['E01.370.600.115.100.160', 'E05.041.124.160', 'G07.100.100.160', 'G07.345.249.314'], ['Z01.252.245.393'], ['E01.370.600.115.100.660', 'E05.041.124.715', 'G07.100.100.660', 'G07.345.249.690'], ['B01.050.500.131.617.720.500.500.750.850']]
|
['Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
visGReMLIN: graph mining-based detection and visualization of conserved motifs at 3D protein-ligand interface at the atomic level.
|
BACKGROUND: Interactions between proteins and non-proteic small molecule ligands play important roles in the biological processes of living systems. Thus, the development of computational methods to support our understanding of the ligand-receptor recognition process is of fundamental importance since these methods are a major step towards ligand prediction, target identification, lead discovery, and more. This article presents visGReMLIN, a web server that couples a graph mining-based strategy to detect motifs at the protein-ligand interface with an interactive platform to visually explore and interpret these motifs in the context of protein-ligand interfaces.RESULTS: To illustrate the potential of visGReMLIN, we conducted two cases in which our strategy was compared with previous experimentally and computationally determined results. visGReMLIN allowed us to detect patterns previously documented in the literature in a totally visual manner. In addition, we found some motifs that we believe are relevant to protein-ligand interactions in the analyzed datasets.CONCLUSIONS: We aimed to build a visual analytics-oriented web server to detect and visualize common motifs at the protein-ligand interface. visGReMLIN motifs can support users in gaining insights on the key atoms/residues responsible for protein-ligand interactions in a dataset of complexes.
|
['Humans', 'Hydrogen Bonding', 'Hydrophobic and Hydrophilic Interactions', 'Ligands', 'Protein Binding', 'Proteins', 'User-Computer Interface']
| 32,164,574
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.282'], ['G02.409'], ['D27.720.470.480'], ['G02.111.679', 'G03.808'], ['D12.776'], ['L01.224.900.910']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Information Science [L]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Replacement of destroyed metacarpal heads by autografting metatarsal heads.
|
A surgical procedure of replacing a destroyed metacarpal head with a metatarsal graft is described. With this method internal fixation with foreign material as well as postoperative immobilisation can be avoided. A follow up of 25 autotransplantations in rheumatoid patients is presented, showing no complications and fair function of the new operated joint.
|
['Adult', 'Aged', 'Arthritis, Rheumatoid', 'Female', 'Finger Joint', 'Humans', 'Male', 'Metacarpophalangeal Joint', 'Metacarpus', 'Metatarsus', 'Middle Aged', 'Postoperative Complications', 'Radiography', 'Wound Healing']
| 6,512,376
|
[['M01.060.116'], ['M01.060.116.100'], ['C05.550.114.154', 'C05.799.114', 'C17.300.775.099', 'C20.111.199'], ['A02.835.583.405.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.583.405.500'], ['A01.378.800.667.572'], ['A01.378.610.250.300.480'], ['M01.060.116.630'], ['C23.550.767'], ['E01.370.350.700'], ['G16.762.891']]
|
['Named Groups [M]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Patient compliance with tenoxicam in family practice.
|
This study evaluated factors that may influence patient compliance and also confirmed tolerability and efficacy of tenoxicam in routine clinical practice. Compliance in 1809 patients was evaluated over a 4-week period by physician pill-counts, patient assessment cards, and, for a subpopulation, by electronically monitored pill vials. In addition, physicians documented patient improvement and side effects after 2 weeks and after 4 weeks of therapy; patients reported satisfaction with therapy and side effects on a weekly basis. A total of 399 physicians provided data on 1809 patients, of whom 84.3% had osteoarthritis, 12.6% had rheumatoid arthritis, and 3.2% had ankylosing spondylitis. The typical patient was a woman (64.9%), white (91.2%), in her 50s (mean age, 57.9 years), with a duration of osteoarthritis of at least 1 year (72.3%). High and similar compliance rates were achieved by patients regardless of age, gender, or diagnostic category. Patient and disease characteristics were similar between compliant and noncompliant patients. Most patients (81.1%) experienced improvement of symptoms after 4 weeks of treatment. A low incidence of side effects (12.6%) was reported, with no significant differences observed among patients with respect to age, gender, or diagnostic category. Product characteristics, such as tolerability, efficacy, and dosing regimen, are more significant factors of compliance than patient or disease characteristics. Tenoxicam's tolerability and clinical effectiveness were confirmed in patients with arthritis in routine clinical practice settings.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Anti-Inflammatory Agents, Non-Steroidal', 'Arthritis', 'Canada', 'Chronic Disease', 'Double-Blind Method', 'Family Practice', 'Female', 'Humans', 'Male', 'Middle Aged', 'Patient Compliance', 'Piroxicam', 'Prospective Studies']
| 7,923,322
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D27.505.696.663.850.014.040.500', 'D27.505.954.158.030', 'D27.505.954.329.030'], ['C05.550.114'], ['Z01.107.567.176'], ['C23.550.291.500'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['H02.403.340.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F01.100.150.750.500.600', 'F01.145.488.887.500.600', 'N05.300.150.800.500.600'], ['D02.886.665.500', 'D03.383.855.500'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
|
Neuropilin-2 expression in papillary thyroid carcinoma: correlation with VEGF-D expression, lymph node metastasis, and VEGF-D-induced aggressive cancer cell phenotype.
|
CONTEXT: Neuropilin-2 (Nrp2) is a coreceptor for vascular endothelial growth factor-D (VEGF-D) that is expressed on the surface of endothelial cells. Recently, Nrp2 was shown to play a role in lymph node metastasis and promotion of cancer cell migration. VEGF-D also promotes lymphangiogenesis, which in turn promotes tumor metastasis.OBJECTIVE: The aim was to study the role of neuropilin-2 in lymph node metastasis in human papillary thyroid carcinoma (PTC).DESIGN: Expression of Nrp2 was studied by immunohistochemistry and the relationship between Nrp2 expression and lymph node metastasis, VEGF-D expression and other established clinicopathological variables were analyzed in PTC. The effects of neutralizing anti-Nrp2 antibody on VEGF-D-induced invasion and migration were assessed in PTC cell lines.RESULTS: Nrp2 expression was observed in 64.3% (36 of 56) of the PTC patients. Nrp2 expression was significantly correlated with lymph node metastasis (P = 0.0216) and VEGF-D expression (P = 0.0034). VEGF-D was shown to promote filopodia formation and cancer cell migration and invasion by K1 and B-CPAP cells. These responses were significantly blocked by neutralizing anti-Nrp2 antibody.CONCLUSION: Nrp2 expression was correlated with lymph node metastasis and VEGF-D expression in PTC. Our data also showed a role for Nrp2 in regulating VEGF-D-induced invasion and migration in vitro.
|
['Adult', 'Aged', 'Antibodies, Neutralizing', 'Carcinoma, Papillary', 'Cell Line, Tumor', 'Cell Movement', 'Female', 'Humans', 'Lymph Nodes', 'Lymphatic Metastasis', 'Male', 'Middle Aged', 'Neuropilin-2', 'Phenotype', 'Thyroid Neoplasms', 'Vascular Endothelial Growth Factor D']
| 21,880,798
|
[['M01.060.116'], ['M01.060.116.100'], ['D12.776.124.486.485.114.244', 'D12.776.124.790.651.114.244', 'D12.776.377.715.548.114.244'], ['C04.557.470.200.360', 'C04.557.470.700.360'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A10.549.400', 'A15.382.520.604.412'], ['C04.697.650.560', 'C23.550.727.650.560'], ['M01.060.116.630'], ['D12.776.543.750.590.750'], ['G05.695'], ['C04.588.322.894', 'C04.588.443.915', 'C19.344.894', 'C19.874.788'], ['D12.644.276.100.800.500', 'D12.776.467.100.800.500', 'D23.529.100.800.500']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Targeted deletion of betaIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes.
|
The spectrin membrane skeleton controls the disposition of selected membrane channels, receptors, and transporters. In the brain betaIII spectrin binds directly to the excitatory amino acid transporter (EAAT4), the glutamate receptor delta, and other proteins. Mutations in betaIII spectrin link strongly to human spinocerebellar ataxia type 5 (SCA5), correlating with alterations in EAAT4. We have explored the mechanistic basis of this phenotype by targeted gene disruption of Spnb3. Mice lacking intact betaIII spectrin develop normally. By 6 months they display a mild nonprogressive ataxia. By 1 year most Spnb3(-/-) animals develop a myoclonic seizure disorder with significant reductions of EAAT4, EAAT1, GluRdelta, IP3R, and NCAM140. Other synaptic proteins are normal. The cerebellum displays increased dark Purkinje cells (PC), a thin molecular layer, fewer synapses, a loss of dendritic spines, and a 2-fold expansion of the PC dendrite diameter. Membrane and expanded Golgi profiles fill the PC dendrite and soma, and both regions accumulate EAAT4. Correlating with the seizure disorder are enhanced hippocampal levels of neuropeptide Y and EAAT3 and increased calpain proteolysis of alphaII spectrin. It appears that betaIII spectrin disruption impairs synaptogenesis by disturbing the intracellular pathways selectively regulating protein trafficking to the synapse. The mislocalization of these proteins secondarily disrupts glutamate transport dynamics, leading to seizures, neuronal damage, and compensatory changes in EAAT3 and neuropeptide Y.
|
['Animals', 'Ataxia', 'Base Sequence', 'Brain', 'DNA Primers', 'Disease Models, Animal', 'Excitatory Amino Acid Transporter 4', 'Female', 'Gene Targeting', 'Humans', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Mice, Knockout', 'Microscopy, Electron, Transmission', 'Nerve Degeneration', 'Phenotype', 'Seizures', 'Spectrin', 'Spinocerebellar Ataxias', 'Synapses']
| 20,231,455
|
[['B01.050'], ['C10.597.350.090', 'C23.888.592.350.090'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A08.186.211'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['D12.776.157.530.200.249.500.500.937', 'D12.776.157.530.450.625.147.937', 'D12.776.157.530.562.374.781.812', 'D12.776.157.530.937.250.500.937', 'D12.776.543.585.200.249.500.500.937', 'D12.776.543.585.450.625.147.937', 'D12.776.543.585.562.374.781.812', 'D12.776.543.585.937.250.500.937'], ['E05.393.335'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['E01.370.350.515.402.580', 'E05.595.402.580'], ['C23.550.737'], ['G05.695'], ['C10.597.742', 'C23.888.592.742'], ['D12.776.220.980', 'D12.776.543.850'], ['C10.228.140.252.190.530', 'C10.228.140.252.700.700', 'C10.228.854.787.875', 'C10.574.500.825.700', 'C10.597.350.090.500.530', 'C16.320.400.780.875'], ['A08.850', 'A11.284.149.165.420.780']]
|
['Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Viewing and sectioning the rat's optic nerve using cheap retractors.
|
A technique for sectioning the optic nerve in rats is described which permits direct viewing of the nerve during surgery. The technique results in no damage to the front of the eye. A procedure for constructing cheap, effective retractors is described.
|
['Animals', 'Denervation', 'Nerve Degeneration', 'Ophthalmologic Surgical Procedures', 'Optic Nerve', 'Optic Nerve Injuries', 'Rats', 'Surgical Instruments', 'Visual Pathways']
| 14,677,600
|
[['B01.050'], ['E04.525.210'], ['C23.550.737'], ['E04.540'], ['A08.800.800.120.680'], ['C10.292.200.750', 'C10.292.700.475', 'C10.900.300.218.550', 'C11.640.530', 'C26.915.300.400.650'], ['B01.050.150.900.649.313.992.635.505.700'], ['E07.858.700'], ['A08.612.220.860']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
An assessment of the in vivo efficacy of the glycogen phosphorylase inhibitor GPi688 in rat models of hyperglycaemia.
|
BACKGROUND AND PURPOSE: Studies in cultured hepatocytes demonstrate glycogen synthase (GS) activation with glycogen phosphorylase (GP) inhibitors. The current study investigated whether these phenomena occurred in vivo using a novel GP inhibitor.EXPERIMENTAL APPROACH: An allosteric GP inhibitor, GPi688, was evaluated against both glucagon-mediated hyperglycaemia and oral glucose challenge-mediated hyperglycaemia to determine the relative effects against GP and GS in vivo.KEY RESULTS: In rat primary hepatocytes, GPi688 inhibited glucagons-mediated glucose output in a concentration dependent manner. Additionally GP activity was reduced and GS activity increased seven-fold. GPi688 inhibited glucagon-mediated hyperglycaemia in both Wistar (65%) & obese Zucker (100%) rats and demonstrated a long duration of action in the Zucker rat. The in vivo efficacy in the glucagon challenge model could be predicted by the equation; % glucagon inhibition=56.9+34.3[log ([free plasma]/rat IC50)], r=0.921). GPi688 also reduced the blood glucose of obese Zucker rats after a 7 h fast by 23%. In an oral glucose tolerance test in Zucker rats, however, GPi688 was less efficacious (7% reduction) than a glycogen synthase kinase-3 (GSK-3) inhibitor (22% reduction), despite also observing activation (by 45%) of GS in vivo.CONCLUSIONS AND IMPLICATIONS: Although GP inhibition can inhibit hyperglycaemia mediated by increased glucose production, the degree of GS activation induced by allosteric GP inhibitors in vivo, although discernible, is insufficient to increase glucose disposal. The data suggests that GP inhibitors might be more effective clinically against fasting rather than prandial hyperglycaemic control.
|
['Animals', 'Blood Glucose', 'Disease Models, Animal', 'Dose-Response Relationship, Drug', 'Glucagon', 'Glucose', 'Glucose Tolerance Test', 'Glycogen Phosphorylase', 'Glycogen Synthase', 'Hepatocytes', 'Hyperglycemia', 'Inhibitory Concentration 50', 'Male', 'Obesity', 'Quinolones', 'Rats', 'Rats, Wistar', 'Rats, Zucker', 'Thiophenes']
| 17,934,512
|
[['B01.050'], ['D09.947.875.359.448.500'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['G07.690.773.875', 'G07.690.936.500'], ['D06.472.699.587.730.500', 'D12.644.548.586.730.500'], ['D09.947.875.359.448'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['D08.811.913.400.450.460.400.186'], ['D08.811.913.400.450.460.375'], ['A11.436.348'], ['C18.452.394.952'], ['E05.940.350', 'G07.690.936.563'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['D03.633.100.810.835'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['B01.050.150.900.649.313.992.635.505.700.550.700'], ['D02.886.778', 'D03.383.903']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Antiprotozoal properties of 16,17-dihydrobrachycalyxolide from Vernonia brachycalyx.
|
Extracts of the leaves from Vernonia brachycalyx showed in vitro activity against Plasmodium falciparum and promastigotes of Leishmania major. The germacrane dilactone 16,17-dihydrobrachycalyxolide (1) which was previously isolated from the aerial parts of the plant was shown to be the major antiplasmodial principle. An X-ray crystallographic analysis established the absolute configuration and some signals in the NMR spectra were reassigned. 16,17-Dihydrobrachycalyxolide (1) elicited a strong antiplasmodial and antileishmanial activity but also a high toxicity against human lymphocytes.
|
['Animals', 'Antiprotozoal Agents', 'Cell Survival', 'Humans', 'Leishmania major', 'Lymphocytes', 'Models, Molecular', 'Molecular Conformation', 'Molecular Structure', 'Plant Extracts', 'Plant Leaves', 'Plants, Medicinal', 'Sesquiterpenes, Germacrane']
| 9,741,304
|
[['B01.050'], ['D27.505.954.122.250.100'], ['G04.346'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.268.475.868.488.405'], ['A11.118.637.555.567', 'A15.145.229.637.555.567', 'A15.382.490.555.567'], ['E05.599.595'], ['G02.111.570.820'], ['G02.111.570', 'G02.466'], ['D20.215.784.500', 'D26.667'], ['A18.024.812'], ['B01.650.560'], ['D02.455.849.765.521.750']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Lower-body determinants of running economy in male and female distance runners.
|
A variety of training approaches have been shown to improve running economy in well-trained athletes. However, there is a paucity of data exploring lower-body determinants that may affect running economy and account for differences that may exist between genders. Sixty-three male and female distance runners were assessed in the laboratory for a range of metabolic, biomechanical, and neuromuscular measures potentially related to running economy (ml·kg(-1)·min(-1)) at a range of running speeds. At all common test velocities, women were more economical than men (effect size [ES] = 0.40); however, when compared in terms of relative intensity, men had better running economy (ES = 2.41). Leg stiffness (r = -0.80) and moment arm length (r = 0.90) were large-extremely largely correlated with running economy and each other (r = -0.82). Correlations between running economy and kinetic measures (peak force, peak power, and time to peak force) for both genders were unclear. The relationship in stride rate (r = -0.27 to -0.31) was in the opposite direction to that of stride length (r = 0.32-0.49), and the relationship in contact time (r = -0.21 to -0.54) was opposite of that of flight time (r = 0.06-0.74). Although both leg stiffness and moment arm length are highly related to running economy, it seems that no single lower-body measure can completely explain differences in running economy between individuals or genders. Running economy is therefore likely determined from the sum of influences from multiple lower-body attributes.
|
['Achilles Tendon', 'Adolescent', 'Arm', 'Biomechanical Phenomena', 'Exercise Test', 'Female', 'Gait', 'Humans', 'Kinetics', 'Leg', 'Male', 'Movement', 'Oxygen Consumption', 'Running', 'Sex Factors', 'Young Adult']
| 24,126,900
|
[['A02.880.176'], ['M01.060.057'], ['A01.378.800.075'], ['G01.154.090', 'G01.374.089'], ['E01.370.370.380.250', 'E01.370.386.700.250', 'E05.333.250'], ['E01.370.600.250', 'G11.427.410.568.900.750'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['A01.378.610.500'], ['G07.568', 'G11.427.410'], ['G03.680'], ['G11.427.410.568.610', 'G11.427.410.698.277.750', 'I03.350.750', 'I03.450.642.845.610'], ['N05.715.350.675', 'N06.850.490.875'], ['M01.060.116.815']]
|
['Anatomy [A]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Estimation of potential arsenic leaching from its phases in excavated sedimentary and metamorphic rocks.
|
It is important that hazardous excavated sedimentary and metamorphic rocks are treated appropriately and reused without posing an environmental risk. Up-flow column leaching tests were conducted to examine whether arsenic leaching behavior varied among five hazardous excavated sedimentary and metamorphic rocks (two mudstones, clay sediment of marine origin, slate, and black schist) and to determine whether the potential amount of arsenic leaching could be estimated based on the arsenic-bearing mineral phases in the rock. Changes in arsenic concentration with pore volume (PV) showed the same pattern across all rock types, except for one that contained an extremely low amount of water-soluble arsenic, exhibiting an initial increase to reach a peak, followed by a decrease. The arsenic amounts leached before and after the PV at which the arsenic concentration peaked, corresponded to 88% ± 20% of the amount of arsenic fraction 1 obtained by sequential extraction and 76% ± 10% of the amount of arsenic fraction 2, respectively, while the potential amount of arsenic leaching corresponded to 65-89% of the summed total of arsenic fractions 1 + 2. These findings indicate that arsenic exhibits the same leaching behavior among different types of hazardous excavated sedimentary and metamorphic rocks except where extremely low amounts of water-soluble arsenic are present and that the potential amount of arsenic leaching can be approximated by calculating the summed total of arsenic fractions 1 + 2, which allows us to estimate the minimum amount of material required for treatments such as immobilization conducted to prevent arsenic leaching.
|
['Arsenic', 'Geologic Sediments', 'Minerals', 'Soil Pollutants', 'Solubility']
| 31,300,943
|
[['D01.268.513.249'], ['G01.311.330', 'G16.500.320'], ['D01.578'], ['D27.888.284.756'], ['G02.805']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Gender Equality, Patriarchal Cultural Norms, and Perpetration of Intimate Partner Violence: Comparison of Male University Students in Asian and European Cultural Contexts.
|
This study examined the relationship between patriarchal cultural norms and violence perpetration by male partners using a subsample of university students in Asia ( n = 784) and Europe ( n = 575) from the International Dating Violence Study (IDVS) data set. Bivariate analyses indicated Asian students scored significantly higher than Europeans on dominance, hostility to women, jealousy, negative attribution, and violence approval as well as perpetration of severe physical assault in dating relationships. Logistic regression models demonstrated that dominance and violence approval were significant predictors of severe physical and psychological aggression against dating partners. Implications for culturally relevant programming for intimate partner violence prevention are discussed.
|
['Adult', 'Asia', 'Cultural Characteristics', 'Europe', 'Family Characteristics', 'Humans', 'Interpersonal Relations', 'Intimate Partner Violence', 'Male', 'Sexism', 'Students', 'Universities']
| 27,378,719
|
[['M01.060.116'], ['Z01.252'], ['I01.076.201.450.324', 'I01.880.853.100.329'], ['Z01.542'], ['F01.829.263.315', 'I01.240.361', 'I01.880.853.150.423', 'N01.224.361', 'N01.824.308', 'N06.850.505.400.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401'], ['I01.198.240.856.575', 'I01.880.735.900.688'], ['F01.145.813.550.750', 'F01.145.813.629.750', 'F01.829.595.750'], ['M01.848'], ['I02.783.830', 'J03.832.830']]
|
['Named Groups [M]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 1
|
Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.
|
OBJECTIVE: One potential mechanism through which obesity exerts adverse effects on the vascular system is by increasing aortic stiffness, a change known to be predictive of increased cardiovascular mortality. The aim of this study was to investigate the pathophysiology that links obesity to aortic stiffening.APPROACH AND RESULTS: Obese (ob/ob) mice were used to examine physical, morphological, and molecular changes in the aorta in response to obesity. ob/ob mice had increased aortic pulse wave velocity and tissue rigidity. ob/ob aorta exhibited decreases of lysyl oxidase (LOX) activity and cross-linked elastin, and increases of elastin fragmentation and elastolytic activity. The aortas of ob/ob mice were surrounded by a significant amount of proinflammatory and pro-oxidative perivascular adipose tissue. In vitro studies revealed that the conditioned medium from differentiated adipocytes or the perivascular adipose tissue of ob/ob mice attenuated LOX activity. Furthermore, inhibition of LOX in wild-type lean mice caused elastin fragmentation and induced a significant increase in pulse wave velocity. Finally, we found that obese humans had stiffer arteries and lower serum LOX levels than do normal-weight humans.CONCLUSIONS: Our results demonstrated that obesity resulted in aortic stiffening in both humans and mice, and established a causal relationship between LOX downregulation and aortic stiffening in obesity.
|
['Adipocytes', 'Adipose Tissue', 'Adult', 'Aminopropionitrile', 'Animals', 'Aorta, Abdominal', 'Case-Control Studies', 'Cell Line', 'Culture Media, Conditioned', 'Cytokines', 'Disease Models, Animal', 'Dose-Response Relationship, Drug', 'Down-Regulation', 'Elastic Modulus', 'Elastin', 'Enzyme Inhibitors', 'Female', 'Humans', 'Inflammation Mediators', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Middle Aged', 'Obesity', 'Oxidative Stress', 'Protein-Lysine 6-Oxidase', 'Pulse Wave Analysis', 'Time Factors', 'Vascular Stiffness']
| 23,413,430
|
[['A11.329.114'], ['A10.165.114'], ['M01.060.116'], ['D02.626.151'], ['B01.050'], ['A07.015.114.056.205'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['A11.251.210'], ['D27.720.470.305.250', 'E07.206.250'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['G07.690.773.875', 'G07.690.936.500'], ['G02.111.240', 'G05.308.200', 'G07.690.773.937'], ['G01.374.590.605'], ['D05.750.078.421', 'D12.776.860.300.350'], ['D27.505.519.389'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D23.469'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['M01.060.116.630'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['G03.673', 'G07.775.750'], ['D08.811.682.664.500.848'], ['E01.370.370.680'], ['G01.910.857'], ['G09.330.940']]
|
['Anatomy [A]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The in vitro effect of monensin on BK polyomavirus replication.
|
The in vitro effect of monensin, a linear polyether, on the infection of Vero cells by BK polyomavirus, was investigated. Data reported in this paper showed an inhibition of BK viral replication by monensin as monitored by immunofluorescence and molecular hybridization. The inhibition of the synthesis of viral nuclear T antigen and the lack of production of viral mRNAs in monensin-treated cells suggest that the effect of this ionophore takes place at the level of the viral DNA delivery, by blocking the uncoating of BK virus or its transport to the nucleus.
|
['Animals', 'Antigens, Viral, Tumor', 'BK Virus', 'Chlorocebus aethiops', 'Humans', 'Ionophores', 'Kinetics', 'Monensin', 'RNA, Messenger', 'RNA, Viral', 'Transcription, Genetic', 'Vero Cells', 'Virus Replication']
| 8,590,386
|
[['B01.050'], ['D23.050.285.062', 'D23.050.327.062'], ['B04.280.210.700.615.100', 'B04.613.204.670.615.100'], ['B01.050.150.900.649.313.988.400.112.199.120.126.110'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D27.505.519.562.374', 'D27.720.395'], ['G01.374.661', 'G02.111.490'], ['D03.383.312.600'], ['D13.444.735.544'], ['D13.444.735.828'], ['G02.111.873', 'G05.297.700'], ['A11.251.210.955', 'A11.436.955'], ['G06.920.925']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Characterization of EHD4, an EH domain-containing protein expressed in the extracellular matrix.
|
To identify proteins that promote assembly of type VI collagen tetramers or stabilize type VI collagen filaments, a two-hybrid screen of a human placenta library was used and a new extracellular protein discovered. The cDNA sequence of the new protein encodes 541 amino acid residues. This cDNA sequence is identical to EHD4, a recently described member of the EH domain family of proteins. Two mRNAs of 4.4 and 3.0 kilobases were present in human skin fibroblasts and most tissues tested but were most prevalent in the heart. The chromosomal localization of the gene for this new protein was determined to be at 15q14-q15. Three polyclonal peptide antibodies were made against synthetic EHD4 peptides. The affinity-purified antibodies were used in immunofluorescent staining of developing limbs and matrices produced by human skin fibroblasts and mouse NIH3T3 fibroblasts in culture. Embryonic rat limb cartilage was strongly stained throughout development, and cultured fibroblasts deposited an extracellular filamentous network containing EHD4. In non-denaturing extracts of fetal bovine cartilage and in human skin fibroblast culture media, two components of approximately 220 and 158 kDa were observed, which, after reduction, migrated as a 56-kDa component on SDS-polyacrylamide gel electrophoresis. EHD4 is the first extracellular matrix protein described that contains an EH domain.
|
['3T3 Cells', 'Amino Acid Sequence', 'Animals', 'Base Sequence', 'Blotting, Northern', 'Blotting, Western', 'Carrier Proteins', 'Cartilage', 'Cattle', 'Cells, Cultured', 'Chromosome Mapping', 'Chromosomes, Human, Pair 15', 'Collagen', 'Collagen Type IV', 'DNA, Complementary', 'DNA-Binding Proteins', 'Electrophoresis, Polyacrylamide Gel', 'Extracellular Matrix', 'Extracellular Matrix Proteins', 'Fibroblasts', 'Fluorescent Antibody Technique, Indirect', 'Gene Library', 'Humans', 'Mice', 'Models, Genetic', 'Molecular Sequence Data', 'Nuclear Proteins', 'Organ Culture Techniques', 'Peptides', 'Placenta', 'Protein Binding', 'Protein Structure, Tertiary', 'RNA, Messenger', 'Radiation Hybrid Mapping', 'Rats', 'Skin', 'Tissue Distribution', 'Two-Hybrid System Techniques']
| 11,533,061
|
[['A11.251.210.100', 'A11.329.228.100'], ['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.196.401.095', 'E05.301.300.074', 'E05.601.100'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['D12.776.157'], ['A02.165', 'A10.165.382'], ['B01.050.150.900.649.313.500.380.271'], ['A11.251'], ['E05.393.183'], ['A11.284.187.520.300.370.385', 'G05.360.162.520.300.370.385'], ['D05.750.078.280', 'D12.776.860.300.250'], ['D12.776.860.300.250.400.100'], ['D13.444.308.497.220', 'D13.444.600.223.500', 'D27.720.470.530.600.223.260'], ['D12.776.260'], ['E05.196.401.402', 'E05.301.300.319'], ['A11.284.295.310'], ['D12.776.860.300'], ['A11.329.228'], ['E01.370.225.500.607.512.240.310', 'E01.370.225.750.551.512.240.310', 'E05.200.500.607.512.240.310', 'E05.200.750.551.512.240.310', 'E05.478.583.375.310'], ['G05.360.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['E05.599.395.397'], ['L01.453.245.667'], ['D12.776.660'], ['E05.481.500.484'], ['D12.644'], ['A16.710'], ['G02.111.679', 'G03.808'], ['G02.111.570.820.709.610'], ['D13.444.735.544'], ['E05.393.183.620.405'], ['B01.050.150.900.649.313.992.635.505.700'], ['A17.815'], ['G03.787.917', 'G07.690.725.949'], ['E05.393.220.870', 'E05.601.690.650', 'E05.601.870']]
|
['Anatomy [A]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
[Activities in social gynaecology for prepartal - prenatal attention (author's transl)].
|
An account is given of what is being done in the GDR in the context of social gynaecology, with reference being made to the subject proper as well as to its position in society and the tasks relating to it. Prepartal attention is described as an example. An appraisal of various concepts of social gynaecology has shown that these have always depended on the social conditions under which they have come into being. Their potentials and outcome are in conformity with those conditions, as well. Attempts have been made to define social gynaecology by the ways it is practised in certain "charitable" institutions or with closer reference to certain groups of people. However, such attempts have proved to be unacceptable to practitioners in the GDR where social gynaecology is considered a discipline of research and education to provide a profound scientific basis for action in reality. It is a social component of public health and an integral element of gynaecology and obstetrics.
|
['Female', 'Germany, East', 'Humans', 'Maternal Health Services', 'Pregnancy', 'Prenatal Care']
| 532,443
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Tangeretin Inhibits Oxidative Stress and Inflammation via Upregulating Nrf-2 Signaling Pathway in Collagen-Induced Arthritic Rats.
|
BACKGROUND/AIMS: Tangeretin (TAN), a major phytochemical in tangerine peels and an important Chinese herb, has multiple biological properties, especially antioxidative and anti-inflammatory effects. However, the mechanisms remain unclear. Based on these findings, the aim of the present study was to assess the antioxidant and anti-inflammatory properties of TAN in bovine type II collagen-induced arthritis rats.METHODS: TAN (50 mg/kg) was given orally once daily for 14 days. The effects of treatment were evaluated by biochemical assay (articular elastase, myeloperoxidase, end products of lipid peroxidation [MDA], antioxidant enzyme, such as superoxide dismutase, catalase, glutathione), nitric oxide, and inflammatory cytokines (interleukin-1â [IL-1â], -IL-10, tumor necrosis factor-alpha [TNF-á], interferon-ã [IFN-ã], and prostaglandin E2 [PGE2]). The protective effects of TAN against rheumatoid arthritis (RA) were evident from the decrease in arthritis scoring. Furthermore, the Nrf-2 signaling pathway was assessed to illustrate the molecular mechanism.RESULTS: TAN had therapeutic effects on RA by decreasing the oxidative stress damage and regulating inflammatory cytokine expression, including suppression of the accumulation of MDA products, decreasing the IL-1â, TNF-á, IFN-ã, and PGE2 levels, enhancing the IL-10 and the activity of antioxidant enzymes, which was through upregulating Nrf-2 signaling pathway.CONCLUSION: TAN might have potential as a therapeutic agent for the treatment of RA.
|
['Animals', 'Anti-Inflammatory Agents', 'Antioxidants', 'Arthritis, Experimental', 'Arthritis, Rheumatoid', 'Catalase', 'Collagen', 'Cytokines', 'Dinoprostone', 'Flavones', 'Glutathione', 'Inflammation', 'Interleukin-10', 'Interleukin-1beta', 'Joints', 'Lipid Peroxidation', 'Male', 'NF-E2-Related Factor 2', 'Nitric Oxide', 'Oxidative Stress', 'Rats', 'Rats, Wistar', 'Signal Transduction', 'Superoxide Dismutase', 'Tumor Necrosis Factor-alpha', 'Up-Regulation']
| 31,344,704
|
[['B01.050'], ['D27.505.954.158'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['C05.550.114.015', 'E05.598.500.249'], ['C05.550.114.154', 'C05.799.114', 'C17.300.775.099', 'C20.111.199'], ['D08.811.682.732.332'], ['D05.750.078.280', 'D12.776.860.300.250'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['D10.251.355.255.550.250.200', 'D23.469.050.175.725.250.200'], ['D03.383.663.283.266.450.260', 'D03.633.100.150.266.450.260'], ['D12.644.456.448'], ['C23.550.470'], ['D12.644.276.374.465.510', 'D12.776.467.374.465.510', 'D23.529.374.465.510'], ['D12.644.276.374.465.010.600', 'D12.644.276.374.500.400.600', 'D12.776.467.374.465.010.600', 'D12.776.467.374.500.400.600', 'D23.529.374.465.131.600', 'D23.529.374.500.400.600'], ['A02.835.583'], ['G02.111.515', 'G03.295.531.587'], ['D12.776.260.108.737', 'D12.776.930.127.737'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['G03.673', 'G07.775.750'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['G02.111.820', 'G04.835'], ['D08.811.682.881'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Dendritic cell-associated B7-H3 suppresses the production of autoantibodies and renal inflammation in a mouse model of systemic lupus erythematosus.
|
B7-H3 immune modulatory molecule has been implicated in the generation and pathogenesis of autoimmune diseases, the mechanism of action is less known. We explored the role of B7-H3 in the induction of autoantibodies and organ-directed inflammation in a murine systemic lupus erythematosus (SLE) model in which the immunization with DNA extracted from activated T cells induced the production of anti-DNA autoantibodies and subsequent glomerulonephritis, two hallmarks of human SLE. Mice deficient of B7-H3 or treated with a B7-H3 specific antibody produced significantly higher levels of anti-DNA autoantibodies and more severe glomerulonephritis than wild-type mice, indicating an inhibitory function of B7-H3 in this model. Interestingly, immunization of mice with DNA-pulsed dendritic cells induced severe SLE symptoms while B7-H3 on dendritic cells is required in this process. Importantly, treatment of mice with recombinant B7-H3Ig fusion protein effectively ameliorated progression of murine SLE, accompanied with decreased level of anti-DNA autoantibodies and alleviated glomerulonephritis, decreased autoantibody deposition and complement deposition in kidney. Our findings implicate a potential role of B7-H3 on dendritic cells in the induction of SLE and as a potential target for the treatment of autoimmune diseases.
|
['Animals', 'Antibodies', 'Antibodies, Antinuclear', 'Autoantibodies', 'B7 Antigens', 'CD4-Positive T-Lymphocytes', 'DNA', 'Dendritic Cells', 'Disease Models, Animal', 'Female', 'Glomerulonephritis', 'Interleukin-6', 'Kidney', 'Lupus Erythematosus, Systemic', 'Lymphocyte Activation', 'Mice', 'Mice, Inbred C57BL', 'Mice, Knockout', 'Recombinant Fusion Proteins']
| 31,113,935
|
[['B01.050'], ['D12.776.124.486.485.114', 'D12.776.124.790.651.114', 'D12.776.377.715.548.114'], ['D12.776.124.486.485.114.323.204', 'D12.776.124.790.651.114.323.204', 'D12.776.377.715.548.114.323.204'], ['D12.776.124.486.485.114.323', 'D12.776.124.790.651.114.323', 'D12.776.377.715.548.114.323'], ['D12.776.467.150', 'D12.776.543.095', 'D23.050.301.285', 'D23.529.168'], ['A11.118.637.555.567.569.200', 'A15.145.229.637.555.567.569.200', 'A15.382.490.555.567.569.200'], ['D13.444.308'], ['A11.066.270', 'A11.436.270', 'A15.382.066.270', 'A15.382.670.260'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['C12.777.419.570.363', 'C13.351.968.419.570.363'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['A05.810.453'], ['C17.300.480', 'C20.111.590'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['D12.776.828.300']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[New paradigms in mental health and the vision from music therapy].
|
This paper aims to describe the vision of mental health from the standpoint of music therapy, framed by the National Law 26.567. First, basic notions about the origins of this discipline are introduced, as well as the criteria that inform its practice and the tools used by this approach, listening, analysis and the intervention in the mental health field. Later, the concepts of music therapy intervention and creative process are highlighted, providing some characteristics of the relationships that may arise between each of them and the mental health.
|
['Creativity', 'Humans', 'Mental Disorders', 'Mental Health', 'Music Therapy']
| 22,880,198
|
[['F01.752.264', 'F02.463.785.302'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F03'], ['F02.418', 'N01.400.500'], ['E02.190.888.500', 'E02.760.169.063.500.440', 'E02.831.440', 'F04.754.549']]
|
['Psychiatry and Psychology [F]', 'Organisms [B]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Nature and nurture in the family physician's choice of practice location.
|
INTRODUCTION: An understanding of the contextual, professional, and personal factors that affect choice of practice location for physicians is needed to support successful strategies in addressing geographic maldistribution of physicians. This study compared two categories of predictors of family practice location in non-metropolitan areas among undergraduate medical students: individual characteristics (nature), and the rural program component of their training program (nurture). The study aimed to identify factors that predict the location of practice 2 years post-residency training and determine the predictive value of combining nature and nurture variables using administrative data from two undergraduate medical education programs.METHODS: Databases were developed from available administrative sources for a retrospective analysis of two undergraduate medical education programs in Canada: Universit? de Sherbrooke (UdeS) and University of British Columbia (UBC). Both schools have a strong mandate to evaluate the impact of their programs on physician distribution. The dependent variable was location of practice 2 years after completing postgraduate training in family medicine. Independent variables included individual and program characteristics. Separate analyses were conducted for each program using multiple logistic regression.RESULTS: The nature and nurture variables considered in the models explained only 21% to 27% of the variance in the eventual location of practice of family physician graduates. For UdeS, having an address in a rural/small-town environment at application to medical school (OR=2.61, 95% CI: 1.24-6.06) and for UBC, location of high school in a rural/small town (OR=4.03, 95% CI: 1.05-15.41), both increased the chances of practicing in a non-metropolitan area. For UdeS the nurture variable (ie length of clerkship in a non-metropolitan area) was the most significant predictor (OR=1.14, 95% CI: 1.067-1.22). For both medical schools, adding a single nurture variable to the model using only nature variables significantly increased the amount of variation accounted for in predicting location of practice in non-metropolitan areas.CONCLUSIONS: Aspects of graduates' rural background increase the chances of practicing in a non-metropolitan area. A third-year clerkship experience in a rural area may increase the chances of non-metropolitan practice. Although the total variation predicted by both nature and nurture variables in this study was small, adding a nurture variable significantly improves the prediction of individuals who will practice in a non-metropolitan area. The fact that total variation predicted was small is likely to be due to the limitations of the administrative databases used. Different strategies are being implemented in each university to improve the quality of existing administrative databases, as well as to collect relevant data about intent-to-practice, training characteristics, and the attitudes, beliefs and backgrounds of students.
|
['Attitude', 'Canada', 'Choice Behavior', 'Education, Medical, Undergraduate', 'Family Practice', 'Female', 'Humans', 'Male', 'Physicians, Family', 'Professional Practice Location', 'Retrospective Studies', 'Rural Health Services', 'Rural Population', 'Workforce', 'Young Adult']
| 21,919,544
|
[['F01.100'], ['Z01.107.567.176'], ['F02.463.785.373.346'], ['I02.358.399.450'], ['H02.403.340.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.526.485.810.770', 'N02.360.810.770'], ['N04.452.758.772'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['N02.421.816'], ['N01.600.725'], ['N04.452.525'], ['M01.060.116.815']]
|
['Psychiatry and Psychology [F]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
Between- and within-observer agreement for expert judgment of peak flow graphs from a working population.
|
Expert judgment of peak flow-time graphs provides an important tool to detect occupational asthma. This technique has mainly been used in clinics to evaluate reversible airflow obstruction and to assess potential work-related patterns. The reproducibility of this technique in an open working population is unknown. Agreement between and within nine experts was studied using peak flow-time graphs of 49 potato-processing workers. All graphs were classified into four categories by the nine experts, while seven experts read ten graphs at two occasions. Thirty-four graphs (69%) were classified as "no airway obstruction" while four graphs (8%) showed "work-related airway obstruction." There was only slight agreement between the nine experts; mean Cohen's kappa (kappa) was 0.19. Agreement within experts was moderate; mean kappa was 0.47 for judging graphs twice. Our results suggest that in a "healthy" working population, judgment of peak flow graphs is not a favorable method for detection of airway obstruction. If this technique is applied in epidemiological studies, judgment of the graphs should be done by more than one expert.
|
['Adult', 'Asthma', 'Food Technology', 'Humans', 'Middle Aged', 'Netherlands', 'Observer Variation', 'Occupational Diseases', 'Peak Expiratory Flow Rate', 'Reproducibility of Results', 'Solanum tuberosum', 'Time Factors']
| 9,830,603
|
[['M01.060.116'], ['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['J01.576.423.850'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['Z01.542.651'], ['E01.354.753', 'N02.421.450.600', 'N05.715.350.150.675', 'N06.850.490.500.250'], ['C24'], ['E01.370.386.700.660.225.600', 'G09.772.650.300.790'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['B01.650.940.800.575.912.250.908.500.725.777'], ['G01.910.857']]
|
['Named Groups [M]', 'Diseases [C]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 1
|
[A double blind study of the treatment of threatened abortion with fenoterol hydrobromide (author's transl)].
|
UNLABELLED: To study the effectiveness of Fenoterol Hydrobromide in the treatment of threatened abortion a double blind trial was performed. A total of 112 patients was examined. A comprehensive statistical analysis was made including Chi square and Student's t-test to provide a valid comparison of both, trial and control groups, in order to eliminate any possible statistical bias, as seen in many of the former studies of this topic.RESULTS: 1. The number of surviving children during the perinatal period did not differ significantly in the placebo and Fenoterol groups. 2. Fenoterol did not influence the course of pregnancy, full term delivery or fetal outcome, unless early abortion occurred. 3. Pregnancies resulting in abortions obviously were not prolonged by Fenoterol treatment. 4. Histology of aborted pregnancies revealed major pathological findings in more than 99% of the cases. So it may be presumed, that pharmacological treatment was bound to fail in these cases.
|
['Abortion, Threatened', 'Double-Blind Method', 'Ethanolamines', 'Female', 'Fenoterol', 'Humans', 'Infant, Newborn', 'Placebos', 'Pregnancy']
| 7,215,765
|
[['C13.703.090'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['D02.033.100.291', 'D02.033.375.291', 'D02.092.063.291'], ['D02.033.100.291.465.300', 'D02.092.063.291.465.300', 'D02.092.311.660.300', 'D02.455.426.559.389.657.166.175.660.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['D26.660', 'E02.785'], ['G08.686.784.769']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Named Groups [M]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The course of negative symptoms over the first five years of treatment: Data from an early intervention program for psychosis.
|
BACKGROUND: Cross-sectional studies suggest that negative symptoms are constituted by separable domains of reduced expressiveness and reduced motivation, but there is little data on the longitudinal course of these symptoms. We examined evidence for differences in the course and correlates of these two domains in a prospective study of patients presenting with a first episode of psychosis.METHODS: Of 132 patients who were followed up for five years, it was possible to monitor reduced expressiveness and motivation on a weekly basis for 127. Information on treatment delay, premorbid adjustment, intellectual functioning, anxiety, depression and psychosocial functioning were also collected.RESULTS: Over the five year follow-up, symptoms of reduced motivation occurred in 95.3% of patients and reduced expressiveness in 68.5%; and deficits in motivation were more likely to be unremitting (15.7%) than expressive deficits (5.5%). There were differences in the correlates of the proportion of time each patient experienced symptoms of each domain. Depression, weeks of full time occupation and weeks on a disability pension were associated with both domains. Anxiety was associated only with diminished motivation. Lower performance IQ; extrapyramidal symptoms (EPS) and dysrhythmic EEG were associated only with proportion of time showing reduced expressiveness.CONCLUSIONS: The prospective data support previous cross-sectional findings that, while these domains of negative symptoms are correlated, they do show differences in prevalence over time and in their correlates.
|
['Basal Ganglia Diseases', 'Cognition Disorders', 'Cross-Sectional Studies', 'Early Intervention, Educational', 'Electroencephalography', 'Female', 'Follow-Up Studies', 'Humans', 'Male', 'Mood Disorders', 'Psychotic Disorders']
| 26,431,791
|
[['C10.228.140.079'], ['F03.615.250'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['N02.421.143.130.320', 'N02.421.726.320'], ['E01.370.376.300', 'E01.370.405.245'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F03.600'], ['F03.700.675']]
|
['Diseases [C]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Maternal onset de novo SH2D1A mutation and lymphocytic choriomeningitis virus infection in a patient with X‑linked lymphoproliferative disease type 1: a case report.
|
X‑linked lymphoproliferative disease type 1 (XLP1) is a rare genetic immunodeficiency disease, which occurs due to germline mutations in the SH2D1A gene. This gene has been reported to encode the adaptor molecule signaling lymphocytic activation molecule‑associated protein XLP1 is generally triggered by the Epstein‑Barr virus (EBV) infection. The present study reported the case of a 4‑year‑old male who presented with a high fever, hypogammaglobulinemia, diffuse lung disease and encephalitis. The patient was infected with the lymphocytic choriomeningitis virus (LCMV), not EBV or any other human herpes virus. The patient was found to carry a SH2D1A c.7G>T/p.A3S mutation, which was inherited from the mother and maternal grandfather, as well as a SH2D1A c.228T>A/p.Y76X mutation, which was identified to be a maternal‑onset de novo mutation at the time of germline development of the patient's mother. To the best of our knowledge, the present study is the first reported case of maternal‑onset XLP1 with a de novo SH2D1A mutation and LCMV infection.
|
['Child, Preschool', 'DNA Mutational Analysis', 'Genes, X-Linked', 'Humans', 'Intracellular Signaling Peptides and Proteins', 'Lymphocytic Choriomeningitis', 'Lymphocytic choriomeningitis virus', 'Lymphoproliferative Disorders', 'Male', 'Mutation', 'Pedigree', 'Radiography, Thoracic', 'Signaling Lymphocytic Activation Molecule Associated Protein', 'Tomography, X-Ray Computed']
| 25,572,984
|
[['M01.060.406.448'], ['E05.393.760.700.300'], ['G05.360.340.024.340.500', 'G05.420.457'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.360', 'D12.776.476'], ['C01.207.245.500.500', 'C01.925.182.550.500', 'C01.925.782.082.580', 'C10.228.228.245.500.500', 'C10.228.614.400.500'], ['B04.820.480.500.070.100.550'], ['C15.604.515', 'C20.683.515'], ['G05.365.590'], ['E05.393.673'], ['E01.370.350.700.730'], ['D12.644.360.024.332', 'D12.776.157.057.166', 'D12.776.476.024.426'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Prevalence and risk factors of Helicobacter pylori infection in Tunisian children: 1055 children in Cap-Bon (northeastern Tunisia).
|
PURPOSE: The aim of this study was to determine the prevalence of Helicobacter pylori infection in a population of schoolchildren six years of age and to identify the risk factors that predispose children to such infection.PATIENTS AND METHODS: A total of 1055 first-grade primary-school pupils were included. Socioeconomic factors, eating habits, gastrointestinal complaints and family history of peptic ulcer or gastric cancer were recorded with a questionnaire. Serum samples were collected to determine H. pylori infection status using ELISA for IgG antibodies.RESULTS: The prevalence of H. pylori infection was 51.4%. On univariate analysis, risk factors for H. pylori infection were household-crowding, lower socioeconomic status, late weaning from bottlefeeding (more than 18 months), bed-sharing and cup-sharing. Symptoms related to infection were abdominal pain and vomiting. On multivariate analysis, household-crowding, late bottle-weaning, bed-sharing and abdominal pain were the only variables that remained strongly associated with H. pylori infection.CONCLUSION: The high prevalence of H. pylori infection in Tunisian children is associated with poor living conditions.
|
['Child', 'Female', 'Helicobacter Infections', 'Helicobacter pylori', 'Humans', 'Male', 'Prevalence', 'Risk Factors', 'Tunisia']
| 18,691,841
|
[['M01.060.406'], ['C01.150.252.400.466'], ['B03.440.500.550', 'B03.660.150.235.500.250.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['Z01.058.266.887']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Midterm results of surgical treatment of systemic ventricular outflow obstruction in Fontan patients.
|
BACKGROUND: Achieving unobstructed blood flow from the systemic ventricle to the aorta is important during the Fontan procedure for complex cyanotic congenital heart disease when there is systemic ventricular outflow obstruction (SVOO). Because SVOO can progress after the Fontan procedure if there is morphologic obstruction, we have adopted a policy of relieving obstructions to systemic blood flow.METHODS: Twenty-five patients were treated by the Fontan procedure with SVOO. Twenty-one patients had undergone prior pulmonary artery banding and 10 patients had undergone prior arch repair. Systemic ventricular outflow obstruction progressed in 5 patients after the Fontan procedure. Main diagnosis was single ventricle in 12, tricuspid atresia in 5, transposition of the great arteries in 4, double-outlet right ventricle in 3, and common atrioventricular canal in 1. Mean age at operation was 6.5 years (range 1 to 15 years) and the average preoperative pressure gradient across the ascending aorta and systemic ventricle was 29 mm Hg (range 0 to 100 mm Hg). The Damus-Kaye-Stansel procedure was performed in 18 patients (double-barrel anastomosis in 13, end to side anastomosis in 5), and subaortic resection or ventricular septal defect or bulboventricular foramen enlargement was performed in 7. Double-barrel anastomosis has been our first choice since 1994, if the pulmonary valve is intact. Follow-up has ranged from 4 months to 14 years (average 5.0 years). Twenty-three of the 25 patients have undergone recatheterization (average 21.4 months later).RESULTS: No early deaths were found; one late death was reported of a patient with single right ventricle (4.0%). The postoperative average pressure gradient was 1.1 mm Hg (0 to 10 mm Hg), and the average right atrial pressure was 14 mm Hg (9 to 20 mm Hg). In all patients who underwent ventricular septal defect or bulboventricular foramen enlargement, regular sinus rhythm was maintained postoperatively. Regarding the Damus-Kaye-Stansel procedure, there was minimal progression of semilunar valve insufficiency except in 1 patient who underwent end-to-side anastomosis with moderate pulmonary regurgitation postoperatively.CONCLUSIONS: The midterm results of the Fontan procedure with SVOO have been satisfactory. Because SVOO might progress after the Fontan procedure if there is morphologic obstruction, an appropriate strategy to relieve obstruction to systemic blood flow should therefore be performed concomitantly with the Fontan procedure.
|
['Adolescent', 'Anastomosis, Surgical', 'Cardiac Surgical Procedures', 'Child', 'Child, Preschool', 'Fontan Procedure', 'Heart Defects, Congenital', 'Humans', 'Infant', 'Treatment Outcome', 'Ventricular Outflow Obstruction']
| 11,899,191
|
[['M01.060.057'], ['E04.035'], ['E04.100.376', 'E04.928.220'], ['M01.060.406'], ['M01.060.406.448'], ['E04.035.410.295', 'E04.100.376.410.295', 'E04.100.376.724.500', 'E04.100.814.868.875.295', 'E04.928.220.370.295', 'E04.928.220.560.500'], ['C14.240.400', 'C14.280.400', 'C16.131.240.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['C14.280.955']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The mTOR effectors 4EBP1 and S6K2 are frequently coexpressed, and associated with a poor prognosis and endocrine resistance in breast cancer: a retrospective study including patients from the randomised Stockholm tamoxifen trials.
|
INTRODUCTION: mTOR and its downstream effectors the 4E-binding protein 1 (4EBP1) and the p70 ribosomal S6 kinases (S6K1 and S6K2) are frequently upregulated in breast cancer, and assumed to be driving forces in tumourigenesis, in close connection with oestrogen receptor (ER) networks. Here, we investigated these factors as clinical markers in five different cohorts of breast cancer patients.METHODS: The prognostic significance of 4EBP1, S6K1 and S6K2 mRNA expression was assessed with real-time PCR in 93 tumours from the treatment randomised Stockholm trials, encompassing postmenopausal patients enrolled between 1976 and 1990. Three publicly available breast cancer cohorts were used to confirm the results. Furthermore, the predictive values of 4EBP1 and p4EBP1_S65 protein expression for both prognosis and endocrine treatment benefit were assessed by immunohistochemical analysis of 912 node-negative breast cancers from the Stockholm trials.RESULTS: S6K2 and 4EBP1 mRNA expression levels showed significant correlation and were associated with a poor outcome in all cohorts investigated. 4EBP1 protein was confirmed as an independent prognostic factor, especially in progesterone receptor (PgR)-expressing cancers. 4EBP1 protein expression was also associated with a poor response to endocrine treatment in the ER/PgR positive group. Cross-talk to genomic as well as non-genomic ER/PgR signalling may be involved and the results further support a combination of ER and mTOR signalling targeted therapies.CONCLUSION: This study suggests S6K2 and 4EBP1 as important factors for breast tumourigenesis, interplaying with hormone receptor signalling. We propose S6K2 and 4EBP1 as new potential clinical markers for prognosis and endocrine therapy response in breast cancer.
|
['Adaptor Proteins, Signal Transducing', 'Antineoplastic Agents, Hormonal', 'Breast Neoplasms', 'Cell Cycle Proteins', 'Drug Resistance, Neoplasm', 'Female', 'Gene Amplification', 'Gene Expression Regulation, Neoplastic', 'Humans', 'Patient Outcome Assessment', 'Phosphoproteins', 'Prognosis', 'RNA, Messenger', 'Recurrence', 'Retrospective Studies', 'Ribosomal Protein S6 Kinases, 70-kDa', 'TOR Serine-Threonine Kinases', 'Tamoxifen']
| 24,131,622
|
[['D12.644.360.024', 'D12.776.157.057', 'D12.776.476.024'], ['D27.505.954.248.169'], ['C04.588.180', 'C17.800.090.500'], ['D12.776.167'], ['G07.690.773.984.395'], ['G05.308.250', 'G05.365.590.310', 'G05.558.315'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N04.761.559.590.399', 'N05.715.360.575.575.399'], ['D12.776.744'], ['E01.789'], ['D13.444.735.544'], ['C23.550.291.937'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D08.811.913.696.620.682.700.862.249', 'D12.644.360.600.249', 'D12.776.476.600.249'], ['D08.811.913.696.620.682.700.931', 'D12.776.476.925'], ['D02.455.426.559.389.150.700.900']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Case-finding for MS prevalence studies in small communities requires a community-based approach.
|
In response to citizen concerns in 5 small Illinois towns, community-based case-finding determined the prevalence of multiple sclerosis (MS). Potential cases were identified through town meetings, publicity, advocacy groups and local volunteer outreach coordinators. Estimated prevalence based on available medical records for self-identifying individuals for 3 of the 5 communities was high (218-231 per 100,000 population) compared to other studies. Scanning databases in medical offices used in many other studies may miss MS cases; yet tracking medical records is labor-intensive and sometimes restricted by privacy guidelines. MS registries could improve case-finding accuracy and efficiency.
|
['Case-Control Studies', 'Female', 'Humans', 'Male', 'Medical Records', 'Multiple Sclerosis', 'Prevalence', 'Registries', 'Residence Characteristics']
| 17,878,739
|
[['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.940.968', 'N04.452.859.564', 'N05.715.360.300.715.500', 'N06.850.520.308.940.968'], ['C10.114.375.500', 'C10.314.350.500', 'C20.111.258.250.500'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.308.970', 'N04.452.859.819', 'N05.715.360.300.715.700', 'N06.850.520.308.970'], ['N01.224.791', 'N06.850.505.400.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Cognitive decline and education in mild dementia.
|
Recent studies suggested that education may modify the clinical expression of dementia and Alzheimer's disease through its association with a brain reserve capacity. We studied whether education would be related to degree of cognitive decline in mild dementia. Equations to estimate premorbid cognitive ability were derived from a representative normative sample of 83 community-dwelling African Americans using age, education, and gender as independent variables and Word List Learning (WLL) and Animal Fluency (AF) scores from the Consortium to Establish a Registry for Alzheimer's Disease (CERAD) neuropsychological test battery as dependent variables. These equations were applied to a second sample of 131 African Americans (22 with dementia, 109 healthy) who completed CERAD test batteries as part of an epidemiologic study of dementia in the community. Differences between obtained and estimated premorbid WLL and AF test scores were calculated and then analyzed in a 2 (Education) x 2 (Diagnosis) ANOVA. A significant interaction association between Education and Diagnosis on WLL scores and a borderline significant interaction on AF scores showed that the high-education demented group had a greater cognitive decline from estimated premorbid levels than the low-education demented group. Thus, at comparable levels of clinical dementia severity, greater cognitive decline occurred in highly educated patients than in low-educated patients.
|
['Aged', 'Aged, 80 and over', 'Cognition Disorders', 'Dementia', 'Educational Status', 'Female', 'Humans', 'Male', 'Neuropsychological Tests']
| 9,443,477
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['F03.615.250'], ['C10.228.140.380', 'F03.615.400'], ['N01.824.196'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F04.711.513']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The epidemic origin and molecular properties of B': a founder strain of the HIV-1 transmission in Asia.
|
OBJECTIVE: To clarify the epidemic origin and molecular properties of the B' subtype that is an important strain in the HIV-1 epidemic in Asia.DESIGN: The genealogical relationship between the B' and B subtype was investigated with two globally representative datasets covering the gag and env regions. B' sequences were identified, from which the epidemic origin, population genetics and the signature mutation sites of the B' subtype were inferred.METHODS: Two globally representative datasets were compiled, using phylogenetic methods. Through coalescent-based analysis, the genealogical relationship between the B' and B subtypes was investigated. The divergence times and population genetic parameters of B' were estimated in a Bayesian framework using Markov Chains Monte Carlo sampling under a relaxed molecular clock method. Additionally, molecular properties of the B' were identified by performing comparative sequence analysis with the HIV-1 M group.RESULTS: About 15 years later after the B subtype began to spread, the B' diverged from the B subtype. The demographic history of B' was reconstructed, and the epidemic of B' was estimated to originate around 1985. Eight and nine distinct signature mutation sites, unique to B', were found around the p17 and V3 regions, respectively.CONCLUSION: Our research is the first large-scale investigation on HIV-1 B' at a global level and provides a deep insight into one of the founder strains of HIV-1 epidemic in Asia. Our results provide an important reference for HIV scientists, public health officials and HIV vaccine designers.
|
['Amino Acid Sequence', 'Asia', 'Base Sequence', 'Disease Outbreaks', 'Evolution, Molecular', 'Genes, env', 'Genes, gag', 'Genetic Variation', 'HIV Envelope Protein gp120', 'HIV Infections', 'HIV-1', 'Humans', 'Molecular Epidemiology', 'Molecular Sequence Data', 'Phylogeny']
| 18,753,865
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['Z01.252'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['N06.850.290'], ['G05.045.250', 'G16.075.250'], ['G05.360.340.024.340.364.875.172', 'G05.360.340.358.024.875.172', 'G05.360.340.358.840.500.172'], ['G05.360.340.024.340.364.875.258', 'G05.360.340.358.024.875.258', 'G05.360.340.358.840.500.258'], ['G05.365'], ['D12.776.964.775.325.164.249', 'D12.776.964.775.562.500.500', 'D12.776.964.970.880.325.164.249', 'D23.050.327.520.350'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['B04.820.650.589.650.350.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.416', 'E05.393.522', 'H01.158.201.636.475.500', 'H01.158.273.343.595.475.500', 'H01.181.122.650.475.550', 'H02.403.720.500.300', 'N06.850.520.470'], ['L01.453.245.667'], ['G05.697', 'G16.075.605', 'L01.100.697']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Geographicals [Z]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
Continuous monitoring of in vivo nitric oxide formation using EPR analysis in biliary flow.
|
A method to continuously monitor the nitric oxide (NO) level in anesthetized rats, using an in vivo trapping reaction of NO by iron-dithiocarbamate complex, is reported. Previously, we developed a method of monitoring NO in bile samples containing an NO complex excreted from the liver (Anal. Biochem. 243, 8-14, 1996). In the present study, we modified the method so that the bile flows directly through the EPR sample cell. Rats were injected with low doses of lipopolysaccharide (LPS) to induce NO formation and were later anesthetized. After cannulation, the bile duct was connected to the inlet of the EPR sample cell and the trapping agent iron complex of D-N-methylglucamine dithiocarbamate (MGD-Fe) was administered. The EPR signal level from NO complex of MGD-Fe in the flowing bile was continuously monitored. Using this method, immediate changes in in vivo NO level in rats were observed following administration of drugs that can affect NO formation. In addition, a continuous intravenous saline containing MGD-Fe made the EPR signal level stable and improved animal condition as well as survival time. Therefore, this method has two merits; (1) one can continuously monitor NO formation until it reaches the maximum level; (2) a rapid change in NO level after intervention can be followed. Using this method, we tested the effect of the substrate L-arginine and inhibitors for NO synthase activity and NO synthase induction. The sensitivity of the present method was tested by monitoring NO formation in rats after exposure to ionizing radiation.
|
['Animals', 'Arginine', 'Bile', 'Cyclic N-Oxides', 'Electron Spin Resonance Spectroscopy', 'Enzyme Inhibitors', 'Gamma Rays', 'Kinetics', 'Lipopolysaccharides', 'Male', 'NG-Nitroarginine Methyl Ester', 'Nitric Oxide', 'Nitric Oxide Synthase', 'Nitrogen Oxides', 'Rats', 'Rats, Sprague-Dawley', 'Whole-Body Irradiation']
| 10,369,181
|
[['B01.050'], ['D12.125.068.050', 'D12.125.095.104', 'D12.125.142.087'], ['A12.200.087'], ['D03.661.243'], ['E05.196.867.519.274'], ['D27.505.519.389'], ['G01.358.500.505.300', 'G01.750.250.300', 'G01.750.750.400'], ['G01.374.661', 'G02.111.490'], ['D09.400.500', 'D09.698.718.450', 'D10.494', 'D23.050.161.616.525', 'D23.946.123.329.500'], ['D12.125.068.050.525', 'D12.125.095.104.525'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['D08.811.682.664.500.772'], ['D01.362.635', 'D01.625.550', 'D01.650.550.587'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['E02.815.814', 'E05.980']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Emtricitabine seminal plasma and blood plasma population pharmacokinetics in HIV-infected men in the EVARIST ANRS-EP 49 study.
|
We aimed to describe blood plasma (BP) and seminal plasma (SP) pharmacokinetics of emtricitabine (FTC) in HIV-1-infected men, assess its penetration in the male genital tract, and evaluate its impact on seminal plasma HIV load (spVL) detection. Men from the EVARIST ANRS EP49 study receiving combined antiretroviral therapy with FTC and with suppressed BP viral load were included in the study. A total of 236 and 209 FTC BP and SP concentrations, respectively, were available. A population pharmacokinetic model was developed with Monolix 4.1.4. The impact of FTC seminal exposure on spVL detection was explored by receiver operating characteristic (ROC) curves and mixed-effects logistic regressions. FTC BP pharmacokinetics was described by a two-compartment model. The addition of an effect compartment with different input and output constants best described FTC SP pharmacokinetics. No covariates were found to explain the variability in SP. FTC exposures (area under the concentration-time curve from 0 to 24 h [AUC0-24]) were higher in SP than in BP (median AUC0-24, 38.04 and 12.95 mg · liter(-1) · h, respectively). The median (range) SP-to-BP AUC0-24 ratio was 2.91 (0.84 to 10.08). Less than 1% of FTC AUC0-24 ratios were lower than 1. The impact of FTC SP AUC0-24 or FTC SP-to-BP AUC0-24 ratio on spVL detection was not significant (P = 0.943 or 0.893, respectively). This is the first population model describing FTC pharmacokinetics simultaneously in both BP and SP. FTC distributes well in the male genital tract with higher FTC concentrations in SP than in BP. FTC seminal plasma exposures were considered efficient in the majority of men.
|
['Adult', 'Anti-HIV Agents', 'Emtricitabine', 'HIV Infections', 'Humans', 'Male', 'Middle Aged', 'Plasma', 'Semen']
| 26,282,407
|
[['M01.060.116'], ['D27.505.954.122.388.077.088'], ['D03.383.742.680.245.500.600', 'D13.570.230.329.525', 'D13.570.685.245.500.600'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A12.207.152.693', 'A12.207.270.695', 'A15.145.693'], ['A12.200.732']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
N-butanol extracts of Morinda citrifolia suppress advanced glycation end products (AGE)-induced inflammatory reactions in endothelial cells through its anti-oxidative properties.
|
BACKGROUND: Advanced glycation end products (AGEs), senescent macroprotein derivatives formed during a normal aging process and acceleratedly under diabetic conditions, play a role in atherosclerotic cardiovascular disease. AGEs cause endothelial cell (EC) damage, an initial trigger for atherosclerosis through the interaction with a receptor for AGEs (RAGE). We have previously shown that n-butanol extracts of Morinda citrifolia (noni), a plant belonging to the family Rubiaceae, block the binding of AGEs to RAGE in vitro. In this study, we examined the effects of n-butanol extracts of noni on reactive oxygen species (ROS) generation and inflammatory reactions on AGE-exposed human umbilical vein ECs (HUVECs).METHODS: HUVECs were treated with 100 ìg/ml AGE-bovine serum albumin (AGE-BSA) or non-glycated BSA in the presence or absence of 670 ng/ml n-butanol extracts of noni for 4 h. Then ROS generation and inflammatory and gene expression in HUVECs were evaluated by dihydroethidium staining and real-time reverse transcription-polymerase chain reaction analyses, respectively. THP-1 cell adhesion to HUVECs was measured after 2-day incubation of AGE-BSA or BSA in the presence or absence of 670 ng/ml n-butanol extracts of noni.RESULTS: N-butanol extracts of noni at 670 ng/ml significantly inhibited the AGE-induced ROS generation and RAGE, intercellular adhesion molecule-1 and plasminogen activator inhibitor-1 gene expressions in HUVECs. AGEs significantly increased monocytic THP-1 cell adhesion to HUVECs, which was also prevented by 670 ng/ml n-butanol extracts of noni.CONCLUSIONS: The present study demonstrated for the first time that N-butanol extracts of noni could suppress the AGE-induced inflammatory reactions in HUVECs through its anti-oxidative properties via blocking of the interaction of AGEs with RAGE. Inhibition of the AGE-RAGE axis by n-butanol extracts of noni may be a novel nutraceutical strategy for the treatment of cardiovascular disease.
|
['1-Butanol', 'Antioxidants', 'Cell Line, Tumor', 'Endothelial Cells', 'Glycation End Products, Advanced', 'Human Umbilical Vein Endothelial Cells', 'Humans', 'Morinda', 'Plant Extracts']
| 28,259,164
|
[['D02.033.415.110.175', 'D10.289.110.175'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['A11.251.210.190', 'A11.251.860.180'], ['A11.436.275'], ['D12.776.643.500'], ['A11.436.275.682'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.650.940.800.575.912.250.456.937.566'], ['D20.215.784.500', 'D26.667']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Evidence of impaired sense of smell in hereditary angioedema.
|
BACKGROUND: Hereditary angioedema (HAE) is an autosomal-dominant disorder resulting from C1-inhibitor (C1INH) deficiency. Smell impairments were found in patients affected with systemic lupus erythematosus, that, similarly to HAE, is characterized by the activation of the classical complement pathway with C4 consumption. In this study, we aimed at evaluating the sense of smell in patients with HAE.METHODS: Thirty patients with HAE and 30 healthy age- and sex-matched controls were evaluated for olfactory functions using the 3-stages Sniffin'-Sticks kit (threshold, discrimination, and identification [TDI]). TDI scores were analyzed according to complement levels (C1INH, C3, C4 and CH50), Beck depression inventory (BDI-II) and danazol treatment.RESULTS: A significant decrease in olfactory function was observed in patients affected with HAE compared with controls in total TDI score (P < 0.001), and in the discrimination (P < 0.001) and identification scores (P = 0.012). Anosmia was present only in patients with HAE (3.3%) who also exhibited more frequently hyposmia (53.3%vs 3.3%, P < 0.0001). Complement levels were reduced in patients with HAE. C4 serum levels showed positive correlation with total TDI score (P < 0.001), and with discrimination (P = 0.002) and identification (P = 0.011) scores. CH50 complement levels showed positive correlation with total TDI score (P < 0.001), and with threshold (P = 0.002) and discrimination (P = 0.011) scores. Sex, age, danazol treatment, BDI-II scores were not different between the patients and controls and did not influence TDI scores significantly.CONCLUSION: Evidence for an impaired sense of smell was found in patients with HAE. The reduction in olfactory function in these cases seems to correlate with complement C4 and CH50 levels. Immune and genetic mechanisms might play a role in this defect.
|
['Adult', 'Angioedemas, Hereditary', 'Case-Control Studies', 'Complement C1 Inhibitor Protein', 'Complement C4', 'Complement Hemolytic Activity Assay', 'Complement Pathway, Classical', 'Female', 'Humans', 'Lupus Erythematosus, Systemic', 'Male', 'Middle Aged', 'Olfaction Disorders', 'Smell']
| 20,649,895
|
[['M01.060.116'], ['C14.907.079.500', 'C16.320.798.500.500', 'C17.800.862.945.066.500', 'C20.543.480.904.066.500', 'C20.673.795.500.500'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['D12.644.861.140.500', 'D12.776.124.486.274.920.250.500', 'D12.776.395.320', 'D12.776.872.140.500'], ['D12.776.124.486.274.350'], ['E01.370.225.812.160.155', 'E01.370.225.812.735.155', 'E05.200.812.160.155', 'E05.200.812.735.155', 'E05.478.594.160.150', 'E05.478.594.760.155'], ['G12.274.698'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C17.300.480', 'C20.111.590'], ['M01.060.116.630'], ['C10.597.751.600', 'C23.888.592.763.550'], ['F02.830.816.643', 'G11.561.790.643']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The distribution of S-100 protein in hyperplastic and neoplastic prostatic epithelium.
|
Specimens from 30 cases of benign prostatic hyperplasia and 75 cases of prostatic carcinoma obtained during suprapubic prostatectomy, transurethal resection of the prostate and radical prostatectomy, were stained immunohistochemically for S-100 protein, prostatic acid phosphatase (PAP), prostatic specific antigen (PSA), neuron specific enolase (NSE) and polyclonal keratin. S-100 protein was positive in 9.3% of prostatic carcinomas and negative in all cases of prostatic hyperplasia. PAP and PSA were positive in all cases, while NSE was positive in 16% of the carcinoma cases. Polyclonal keratin was positive in both cell layers of the double layered hyperplastic prostatic epithelium with a more intense staining pattern in the outer cell layer. The authors believe that the S-100 protein immunoreactivity observed in some prostatic carcinomas, reflecting the change in the functional status of the neoplastic cells, might be of prognostic significance. They also emphasize the non-myoepithelial nature of the outer cell layer of the double layered prostatic epithelium.
|
['Epithelium', 'Humans', 'Male', 'Prostatic Hyperplasia', 'Prostatic Neoplasms', 'S100 Proteins']
| 11,229,645
|
[['A10.272'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.294.565.500'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750'], ['D12.776.157.125.750', 'D12.776.631.655']]
|
['Anatomy [A]', 'Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Differential spontaneous folding of mycolic acids from Mycobacterium tuberculosis.
|
Mycolic acids are structural components of the mycobacterial cell wall that have been implicated in the pathogenicity and drug resistance of certain mycobacterial species. They also offer potential in areas such as rapid serodiagnosis of human and animal tuberculosis. It is increasingly recognized that conformational behavior of mycolic acids is very important in understanding all aspects of their function. Atomistic molecular dynamics simulations, in vacuo, of stereochemically defined Mycobacterium tuberculosis mycolic acids show that they fold spontaneously into reproducible conformational groupings. One of the three characteristic mycolate types, the keto-mycolic acids, behaves very differently from either á-mycolic acids or methoxy-mycolic acids, suggesting a distinct biological role. However, subtle conformational behavioral differences between all the three mycolic acid types indicate that cooperative interplay of individual mycolic acids may be important in the biophysical properties of the mycobacterial cell envelope and therefore in pathogenicity.
|
['Hydrophobic and Hydrophilic Interactions', 'Molecular Conformation', 'Molecular Dynamics Simulation', 'Mycobacterium tuberculosis', 'Mycolic Acids', 'Principal Component Analysis', 'Surface Properties', 'Thermodynamics']
| 24,362,064
|
[['G02.409'], ['G02.111.570.820'], ['E05.599.595.500', 'G02.111.570.895', 'L01.224.160.500'], ['B03.510.024.962.500.702', 'B03.510.460.400.410.552.552.702'], ['D02.241.511.621', 'D10.251.572'], ['E05.318.740.562'], ['G02.860'], ['G01.906']]
|
['Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Organisms [B]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Global profiling of the circadian transcriptome using microarrays.
|
Circadian rhythms are biological cycles with a period length of approximately 24 h that are generated by endogenous clocks. The application of microarrays for high-throughput transcriptome analysis has led to the insight that substantial portions of the transcriptomes of both humans and many model organisms are clock-regulated. In a typical circadian time course microarray experiment, samples are collected from organisms maintained in constant environmental conditions, gene expression at each time point is determined using microarrays, and finally clock-regulated transcripts are identified using statistical algorithms. Here, we describe how to design the experiment, process RNA, determine expression profiles using ATH1 microarrays, and use a nonparametric statistical algorithm named JTK_CYCLE in order to identify circadian-regulated transcripts in Arabidopsis. This basic procedure can be modified to identify clock-regulated transcripts in different organisms or using different expression analysis platforms.
|
['Algorithms', 'Circadian Clocks', 'Computational Biology', 'Gene Expression Profiling', 'Transcriptome']
| 24,792,043
|
[['G17.035', 'L01.224.050'], ['G07.180.562.094.500'], ['H01.158.273.180', 'L01.313.124'], ['E05.393.332'], ['G02.111.873.750', 'G05.297.700.750', 'G05.360.920']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Syphilitic lymphadenopathy. Histology and human immunodeficiency virus status.
|
Few reports on syphilitic lymphadenopathy have appeared in 20 years, and none have compared findings in patients with and without human immunodeficiency virus (HIV) infection, despite the recent epidemic spread of syphilis and HIV. Twelve cases of syphilitic lymphadenopathy were studied and grouped according to HIV status. Patients were 21 to 62 years old (median, 29 years); 7 were men, 5 were women. Biopsy sites were cervical (7 cases), inguinal (4), and axillary (1) lymph nodes. All patients had evidence of syphilis. Rapid plasma reagin titers ranged from 1:32 to 1:512. Treponemal hemagglutination was positive in all cases tested. Spirochetes were found with Steiner staining in 2 cases. HIV testing was positive in 4, negative in 2, and unknown in 6 cases. Lymph nodes were enlarged and often fragmented due to capsular fibrosis and chronic inflammation, with focal obliteration of the subcapsular sinus. Follicular and interfollicular hyperplasia was seen in all cases and was usually marked, with prominent vascular proliferation, plasma cells, immuno-blasts, histiocytes, and occasional neutrophils. Follicle lysis and granulomas suggestive of unconfirmed toxoplasmosis were each seen in 1 case, and Kaposi sarcoma in 2, all in HIV-positive patients. Lymphoplasmacytic infiltration was marked, especially in interfollicular areas, with peri-vascular plasma cell cuffing in all cases and obliterative endarteritis in about half (7 of 12, 56%). Immunostaining for CD45RO (UCHL-1), CD20 (L26), kappa, lambda, and CD68 (Kp-1) revealed a mixed population of T cells, polyclonal B cells, and interfollicular histiocytes. Distribution of T and B cells (immunoarchitecture) was essentially normal and similar in all cases, regardless of HIV status. Syphilis produces essentially identical findings in lymph nodes in both HIV-positive and HIV-negative patients. The morphologic findings described should prompt evaluation for infection with Treponema pallidum and, in light of the current epidemic, HIV.
|
['Adult', 'Female', 'HIV Seronegativity', 'HIV Seropositivity', 'Humans', 'Immunohistochemistry', 'Lymphatic Diseases', 'Male', 'Middle Aged', 'Retrospective Studies', 'Syphilis']
| 10,478,137
|
[['M01.060.116'], ['G12.400'], ['C01.221.250.875.500', 'C01.221.812.640.400.500', 'C01.778.640.400.500', 'C01.925.782.815.616.400.500', 'C01.925.813.400.500', 'C20.673.480.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['C15.604'], ['M01.060.116.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['C01.150.252.400.794.840.500', 'C01.150.252.400.840.500', 'C01.150.252.734.859', 'C01.221.812.281.859', 'C01.778.281.859', 'C12.294.668.281.859', 'C13.351.500.711.281.859']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
FSH receptor gene polymorphisms in fertile and infertile Italian men.
|
In women, single nucleotide polymorphisms (SNP) of the FSH receptor (FSHR) gene influence FSH concentrations and the sensitivity of the FSHR to FSH in vivo. In contrast, the significance of FSHR R gene SNP in the male is poorly understood. To this aim, the possible role of three FSHR SNP was evaluated in male infertility. SNP in exon 10 (codon 307 and 680) and in the core promoter region (at position -29) of the FSHR gene were analysed by polymerase chain reaction-restriction fragment length polymorphism technique in 150 men representative of the general population, 107 proven fathers, 92 normozoospermic controls, and 215 infertile patients classified according to sperm parameters (38 azoospermia, 53 severe oligozoospermia, 48 moderate oligozoospermia, and 76 slight oligozoospermia). Reproductive hormones were measured in infertile males and normozoospermic controls. No significant difference was found in allelic variants frequency and genotype distribution between each category of subjects when analysing the FSHR exon 10 SNP alone and in combination with the SNP at position -29. Serum FSH concentrations and other andrological parameters did not differ between subjects with different genotype within each group. The data showed that in the Italian population, FSHR genotypes have no influence on FSH concentrations both in normal and infertile males and do not associate with spermatogenetic impairment.
|
['Alleles', 'Base Sequence', 'DNA', 'Exons', 'Female', 'Fertility', 'Follicle Stimulating Hormone, Human', 'Humans', 'Infertility, Male', 'Italy', 'Male', 'Oligospermia', 'Polymorphism, Single Nucleotide', 'Promoter Regions, Genetic', 'Receptors, FSH']
| 17,169,197
|
[['G05.360.340.024.340.030'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D13.444.308'], ['G05.360.340.024.340.137.232'], ['G08.686.210'], ['D06.472.699.631.525.343.288.625'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.294.365.700'], ['Z01.542.489'], ['C12.294.365.700.508'], ['G05.365.795.598'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['D12.776.543.750.695.250', 'D12.776.543.750.720.600.370', 'D12.776.543.750.750.555.370', 'D12.776.543.750.750.660.350.300']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
|
Benign vascular tumours of the Mediastinum: presentation of three cases and review of the literature.
|
Two patients with epithelioid haemangioendothelioma and one patient with multiple cavernous haemangiomas of the mediastinum, pharynx and larynx, are herein presented. Haemothorax as initial manifestation of the tumour was observed in one of them. Epithelioid haemangioendotheliomas were radically removed in both cases. Because of the absence of a well defined capsule and the huge extension, the cavernous mediastinal haemangioma was not resected. However the patient was successfully treated by administration of corticosteroids. Clinicopathologic characteristics of these benign forms of vascular tumours are discussed and treatment options are suggested.
|
['Adolescent', 'Adult', 'Antineoplastic Agents, Hormonal', 'Female', 'Hemangioendothelioma, Epithelioid', 'Hemangioma, Cavernous', 'Humans', 'Laryngeal Neoplasms', 'Mediastinal Neoplasms', 'Pharyngeal Neoplasms', 'Prednisolone', 'Radiography']
| 8,775,864
|
[['M01.060.057'], ['M01.060.116'], ['D27.505.954.248.169'], ['C04.557.645.375.370.380'], ['C04.557.645.375.385', 'C14.907.454.385', 'C15.378.463.515.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.443.665.481', 'C08.360.369', 'C08.785.481', 'C09.400.369', 'C09.647.481'], ['C04.588.894.479', 'C08.846.187.580'], ['C04.588.443.665.710', 'C07.550.745', 'C09.647.710', 'C09.775.549'], ['D04.210.500.745.432.769.795'], ['E01.370.350.700']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Comparison of the effects of pharmacological therapy and a low-sodium diet on mild hypertension.
|
1. The effect of a low-sodium diet and pharmacological therapy has been compared in eighty-one patients with mild hypertension. 2. Both pharmacological therapy and a low-sodium diet reduced lying and standing systolic and diastolic blood pressure significantly.
|
['Adult', 'Aged', 'Chlorthalidone', 'Clonidine', 'Humans', 'Hypertension', 'Middle Aged', 'Sodium Chloride']
| 1,071,693
|
[['M01.060.116'], ['M01.060.116.100'], ['D02.065.884.365', 'D02.455.426.559.389.134.500', 'D02.478.600.500', 'D02.522.223.500', 'D02.886.590.700.365', 'D03.633.100.513.750.500'], ['D03.383.129.308.436.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['M01.060.116.630'], ['D01.210.450.150.875', 'D01.857.650']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Premenstrual mastodynia: objective signs (author's transl)].
|
Mammary congestion is mainly a painful syndrome, principally related to diffuse edema of the gland, the radiological signs of which are described in this report. Excellent validity can be obtained by a trained observer of these signs. In contrast, however, thermographic signs are of less value, probably due to the presence of a more or less abundant superficial edema. This interferes with thermal propagation towards the skin covering, and could be the cause of the superficial hypothermia which is recorded in one third of patients with definite clinical congestion. Hormonal disturbances are observed frequently enough in this syndrome for them to be considered responsible for a large proportion of the congestive and edemaotus phenomena. Observation of these signs enables a choice of therapy to be made, but this is not discussed in this report.
|
['Adult', 'Breast Diseases', 'Female', 'Humans', 'Mammography', 'Menstruation', 'Middle Aged', 'Pain', 'Progesterone', 'Prolactin', 'Thermography']
| 536,962
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[The observation of local immune response in the lungs from antigen-sensitized and challenged mice].
|
OBJECTIVE: To evaluate the local immune response after allergic sensitization and challenge in the lungs of mice.METHODS: C57BL/6 mice were sensitized with keyhole limpet hemocyanin (KLH) by intratracheal instillation and challenged 2 approximately 4 weeks later. Bronchoalveolar lavage fluid (BALF) and plasma were collected and cells from the lung-associated lymph node (LALN), the lungs and the spleen were cultured and collected. Anti-KLH IgA, total IgA and albumin levels were measured by ELISA.RESULTS: Intratracheal instillation of KLH induced local BAL antigen-specific IgA response, and further challenge expanded this response. The ratio of anti-KLH IgA/albumin in BALF was significantly higher than that in serum after both sensitization and challenge by KLH. In vitro, cells from the LALN and the lungs released anti-KLH IgA after sensitization and challenge by KLH, but spleen cells released lower levels of anti-KLH IgA compared to the LALN and the lungs.CONCLUSIONS: Allergic sensitization by intratracheal instillation of KLH into the mouse lungs induced a local pulmonary response of specific IgA, and it was amplified by challenge with KLH. The accumulation of anti-KLH IgA in the respiratory lumen was the result of local production but not the result of simple transudation or leakage from the serum. The LALN and the lung lymphocytes were major sources of anti-specific IgA.
|
['Animals', 'Antibodies', 'Enzyme-Linked Immunosorbent Assay', 'Hemocyanins', 'Immunoglobulin A', 'Lung', 'Male', 'Mice', 'Mice, Inbred C57BL', 'Spleen']
| 11,953,113
|
[['B01.050'], ['D12.776.124.486.485.114', 'D12.776.124.790.651.114', 'D12.776.377.715.548.114'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['D12.776.093.375', 'D12.776.556.462', 'D23.767.482'], ['D12.776.124.486.485.114.619.026', 'D12.776.124.790.651.114.619.026', 'D12.776.377.715.548.114.619.026'], ['A04.411'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['A10.549.700', 'A15.382.520.604.700']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Ocular complications of porphyria. A case of G?nther's disease].
|
On a case of G?nther congenital porphyria with late ophthalmic complications, the authors study clinical and haematological manifestations of this disease (clinical signs are related to light sensibilisation: sclerodactily, hyperpigmentation, non cicatrisating wounds, long standing scars of reposed areas; they begin in the early childhood by hemolytic anemia and emission of dark port urines too). The various porphyries are then studied and their ophthalmologic signs are related either to the light sensibilisation either to angiopathy and neuropathy (acute porphyria). These are always spontaneously reversible after the acute onset; on the contrary, light sensibilisation involvements go worth and worth and in some cases (G?nther disease; late cutaneous porphyria) induce irreversible damages: they begin by eyelids and conjunctival scars then involve scleral and corneal tissues (scleral malacia perforans). In fact, they have a bad prognosis: ophthalmologic surgical repair, after a little success, fails, because of the non cicatrisating tissues. Best treatment is preventive: --in case of light sensibilisation: eviction of sunlight or light sensibilisating drugs; --in case of acute porphyria: eviction of some drugs (barbiturates); surgical action.
|
['Adolescent', 'Adult', 'Child', 'Child, Preschool', 'Eye Diseases', 'Female', 'Humans', 'Infant', 'Infant, Newborn', 'Liver Diseases', 'Male', 'Middle Aged', 'Photosensitivity Disorders', 'Porphyrias', 'Skin Diseases']
| 7,169,506
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.406'], ['M01.060.406.448'], ['C11'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['C06.552'], ['M01.060.116.630'], ['C17.800.600'], ['C18.452.811'], ['C17.800']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Children who become active.
|
OBJECTIVES: This article examines factors associated with children aged 4 to 11 becoming and remaining active, and how this differed according to their weight.DATA SOURCE: The data are from the National Longitudinal Survey of Children and Youth: cycle 1 (1994/95) for the cross-sectional analysis, and cycles 1, 2 and 3 (1994/95 to 1998/99) for the longitudinal analysis.ANALYTICAL TECHNIQUES: Estimates of physical activity levels in 1994/95 among acceptable-weight and overweight/obese children are presented by age, sex and selected activities (TV viewing, playing computer/video games, and hours of physical education at school). Logistic regression models were constructed for children who were inactive in 1994/95, focusing on the selected activities as predictors of adopting and maintaining an active lifestyle.MAIN RESULTS: Factors associated with children adopting and maintaining an active lifestyle differed, depending on their weight. For overweight/obese children, but not for acceptable-weight children, a relatively high number of physical education hours was predictive of becoming physically active, while frequent TV viewing lowered the odds.
|
['Body Mass Index', 'Canada', 'Child', 'Child Welfare', 'Child, Preschool', 'Exercise', 'Family Characteristics', 'Female', 'Health Behavior', 'Health Promotion', 'Humans', 'Longitudinal Studies', 'Male', 'Obesity', 'Physical Education and Training', 'Prevalence', 'Socioeconomic Factors', 'Television']
| 14,768,291
|
[['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['Z01.107.567.176'], ['M01.060.406'], ['I01.880.787.293'], ['M01.060.406.448'], ['G11.427.410.698.277', 'I03.350'], ['F01.829.263.315', 'I01.240.361', 'I01.880.853.150.423', 'N01.224.361', 'N01.824.308', 'N06.850.505.400.400'], ['F01.145.488'], ['I02.233.332.445', 'N02.421.726.407.579'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['C18.654.726.500', 'C23.888.144.699.500', 'E01.370.600.115.100.160.120.699.500', 'G07.100.100.160.120.699.500'], ['I02.233.543'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['I01.880.853.996', 'N01.824'], ['J01.897.280.500.898', 'L01.178.590.875', 'L01.178.820.090.898', 'L01.178.847.823']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Geographicals [Z]', 'Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Diseases [C]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 1
|
Distinctive regulation of contact activation by antithrombin and C1-inhibitor on activated platelets and material surfaces.
|
Activated human plate lets trigger FXII-mediated contact activation, which leads to the generation of FXIIa-antithrombin (AT) and FXIa-AT complexes. This suggests that contact activation takes place at different sites, on activated platelets and material surfaces, during therapeutic procedures involving biomaterials in contact with blood and is differentially regulated. Here we show that activation in platelet-poor plasma, platelet-rich plasma (PRP), and whole blood induced by glass, kaolin, and polyphosphate elicited high levels of FXIIa-C1-inhibitor (C1INH), low levels of FXIa-C1INH and KK-C1INH, and almost no AT complexes. Platelet activation, in both PRP and blood, led to the formation of FXIIa-AT, FXIa-AT, and kallikrein (KK)-AT but almost no C1INH complexes. In severe trauma patients, FXIIa-AT and FXIa-AT were correlated with the release of thrombospondin-1 (TSP-1) from activated platelets. In contrast, FXIIa-C1INH complexes were detected when the FXIIa-AT levels were low. No correlations were found between FXIIa-C1INH and FXIIa-AT or TSP-1. Inhibition of FXIIa on material surfaces was also shown to affect the function of aggregating platelets. In conclusion, formation of FXIIa-AT and FXIIa-C1INH complexes can help to distinguish between contact activation triggered by biomaterial surfaces and by activated platelets. Platelet aggregation studies also demonstrated that platelet function is influenced by material surface-mediated contact activation and that generation of FXIIa-AT complexes may serve as a new biomarker for thrombotic reactions during therapeutic procedures employing biomaterial devices.
|
['Antithrombins', 'Biocompatible Materials', 'Blood Platelets', 'Complement C1 Inactivator Proteins', 'Enzyme-Linked Immunosorbent Assay', 'Humans', 'Platelet Activation', 'Thrombospondin 1']
| 19,783,299
|
[['D27.505.519.389.745.800.449', 'D27.505.954.502.119.500'], ['D25.130', 'D27.720.102.130', 'J01.637.051.130'], ['A11.118.188', 'A15.145.229.188'], ['D12.644.861.140', 'D12.776.124.486.274.920.250', 'D12.776.872.140'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G09.188.390.600'], ['D12.776.395.550.895.800', 'D12.776.543.550.895.800']]
|
['Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Some safety aspects of Salmonella vaccines for poultry: in vivo study of the genetic stability of three Salmonella typhimurium live vaccines.
|
Live vaccine strains of Salmonella should be avirulent, immunogenic and genetically stable. Some isolates of three commercially available live vaccine strains of Salmonella typhimurium, sampled during a study on their persistence in a vaccinated flock of chickens, were analyzed for genetic stability using macrorestriction analysis of their genome. Two out of the three vaccine strains showed genetic instabilities. Two of the 51 isolates of Zoosaloral vaccine strain and nine of the 32 analyzed isolates of chi(3985), a genetically modified organism, were variants and showed different macrorestriction profiles.
|
['Animals', 'Bacteriophage Typing', 'Chickens', 'Deoxyribonucleases, Type II Site-Specific', 'Electrophoresis, Gel, Pulsed-Field', 'Genotype', 'Poultry Diseases', 'Restriction Mapping', 'Salmonella Infections, Animal', 'Salmonella Vaccines', 'Salmonella typhimurium', 'Vaccines, Attenuated']
| 11,040,436
|
[['B01.050'], ['E01.370.225.875.150.125.150', 'E05.200.875.150.125.150'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['D08.811.150.280.260', 'D08.811.277.352.335.350.300.260', 'D08.811.277.352.355.325.300.260'], ['E05.196.401.220', 'E05.301.300.220'], ['G05.380'], ['C22.131.728'], ['E05.393.183.620.650', 'E05.393.712'], ['C01.150.252.400.310.821.706', 'C22.812'], ['D20.215.894.135.685'], ['B03.440.450.425.800.200.825', 'B03.660.250.150.710.160.760'], ['D20.215.894.811']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Recent trends in specular light reflectance beyond clinical fluorescence diagnosis.
|
Under specific light illumination, particularly ultraviolet (UV) and near-UV light stimulation, the skin produces both specular light reflectance and, possibly, specific fluorescent emission. These properties offer diagnostic clues and disclose some peculiar functions of the skin. A series of superficial infections (erythrasma, some tinea capitis types, tinea/pityriasis versicolor, dermatophytoses, etc.) and pilosebaceous follicles enriched in Propionibacterium spp show fluorescence. This latter characteristic is downgraded or lost while on some anti-acne treatments. A quenching effect of fluorescence is observed following the application of sunscreens. The (pre)neoplastic areas prepared for methylaminolevulinate photodynamic therapy (MAL-PDT) show reddish fluorescence following drug metabolisation producing porphyrins by the abnormal activated cells. Of note, when using a recording sensitive CCD camera instead of casual visual observation, skin fluorescence may be superimposed on the specular reflectance of the incident light. With the current technology, these situations are not distinguished with confidence. Any harsh and scaly lesion appears brighter following yellowish specular light reflectance. Stratum corneum samplings collected on clear self-adhesive discs or cyanoacrylate skin surface strippings are conveniently examined ex vivo, taking advantage of the same optical properties.
|
['Dermatology', 'Dermoscopy', 'Fluorescence', 'Gram-Positive Bacterial Infections', 'Humans', 'Keratosis, Actinic', 'Photochemotherapy', 'Propionibacterium acnes', 'Skin Diseases', 'Skin Neoplasms']
| 21,411,414
|
[['H02.403.225'], ['E01.370.350.515.277.250', 'E05.595.185.250'], ['G01.358.500.505.650.665.500', 'G01.590.540.665.500'], ['C01.150.252.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.834.450', 'C17.800.428.570'], ['E02.186.500', 'E02.319.685', 'E02.774.722'], ['B03.510.024.990.600.600', 'B03.510.460.400.400.600.600.600'], ['C17.800'], ['C04.588.805', 'C17.800.882']]
|
['Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Increased sensitivity and accumulation of estradiol in the rat oviduct during early pregnancy.
|
We have previously reported that a single injection of estradiol-17 beta (E2) given on day 3 of pregnancy (P3) is far more effective for accelerating oviductal transport in the rat, than treatment given on day 1 (P1). In order to quantify this change, dose-response curves were established for six different doses of E2 (range 0.031 to 1.00 micrograms per animal) given on P1, P2 or P3. In addition, a possible mechanism was explored by comparing the plasmatic and oviductal levels of E2 between 30 and 180 min following treatment with E2 on P1 or P3. As the interval from ovulation to treatment was increased, the transport of a larger number of embryos was accelerated and a smaller dose was required. The minimal effective dose decreased 30-fold from P1 to P3, the oviducts accumulated 20% to 90% more E2 on P3 than on P1, tissue levels were 6- to 48-fold higher than plasma levels and the latter did not differ between P1 and P3. It is concluded that the oviduct exhibits increased sensitivity and responsiveness to E2 on P3 and this is associated with greater accumulation of the hormone in the organ, not attributable to higher E2 plasma levels.
|
['Animals', 'Dose-Response Relationship, Drug', 'Estradiol', 'Female', 'Ovum Transport', 'Pregnancy', 'Pregnancy, Animal', 'Rats', 'Rats, Sprague-Dawley', 'Time Factors', 'Tissue Distribution']
| 7,647,816
|
[['B01.050'], ['G07.690.773.875', 'G07.690.936.500'], ['D04.210.500.365.415.248', 'D06.472.334.851.437.500'], ['G04.198.700', 'G08.686.784.277.360'], ['G08.686.784.769'], ['G08.686.784.769.498'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['G01.910.857'], ['G03.787.917', 'G07.690.725.949']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Scanning electron microscopy of urinary stone as a diagnostic tool.
|
On the basis of an extensive electron microscopic study of human urinary stones, a step by step method of stone analysis by scanning electron microscopy (SEM) morphology was developed. A preliminary blind study of 100 consecutive stones demonstrated that the method requires minimum amounts of training and is highly accurate. With several hours of training, accuracy of the stone analysis by SEM morphology alone exceeded 95%. The data indicate that, with further refinements, SEM stone analysis can be accomplished expeditiously and exceed other methods known for stone analysis with economy and accuracy.
|
['Crystallization', 'Humans', 'Methods', 'Microscopy, Electron, Scanning', 'Urinary Calculi']
| 6,523,056
|
[['E05.196.300', 'G02.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.581'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['C12.777.967.500', 'C13.351.968.967.500', 'C23.300.175.850']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Hypereosinophilic syndrome in a child mosaic for a congenital triplication of the short arm of chromosome 8.
|
An 11-year-old boy with mental retardation, malformations, and the mosaic karyotype 47,XY,+i(8p)/46,XY presented with fever, headache and petechiae. Peripheral blood WBC was 190 x 10(9)/l; and contained > 90% mature eosinophils. Cytogenetic analysis of the eosinophils revealed no aberrations except the constitutional karyotype. The patient was diagnosed as having a hypereosinophilic syndrome. Shortly after initiation of therapy he died from extensive mural thrombi of the heart and thrombi of several other organs. This is the first case of congenital triplication of the short arm of chromosome 8 associated with hypereosinophilic syndrome, suggesting involvement of genes on chromosome 8p in the regulation of eosinopoiesis.
|
['Child', 'Chromosomes, Human, Pair 8', 'Coronary Thrombosis', 'Eosinophils', 'Fatal Outcome', 'Humans', 'Hypereosinophilic Syndrome', 'Karyotyping', 'Male', 'Microscopy, Electron', 'Mosaicism', 'Trisomy']
| 9,029,027
|
[['M01.060.406'], ['A11.284.187.520.300.325.340', 'G05.360.162.520.300.325.340'], ['C14.280.647.250.290', 'C14.907.355.830.220', 'C14.907.585.250.290'], ['A11.118.637.415.345', 'A11.627.340.345', 'A15.145.229.637.415.345', 'A15.382.490.315.251'], ['E05.318.308.985.550.325', 'N01.224.935.698.201', 'N06.850.505.400.975.550.325', 'N06.850.520.308.985.550.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C15.378.553.231.549'], ['E01.370.225.500.385.315', 'E05.200.500.385.315', 'E05.242.385.315', 'E05.393.285.475'], ['E01.370.350.515.402', 'E05.595.402'], ['G05.365.590.175.595'], ['C23.550.210.050.750', 'C23.550.210.182.500', 'G05.365.590.175.050.750', 'G05.365.590.175.183.500', 'G05.700.131.750']]
|
['Named Groups [M]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Semiparametric models of time-dependent predictive values of prognostic biomarkers.
|
Rigorous statistical evaluation of the predictive values of novel biomarkers is critical prior to applying novel biomarkers into routine standard care. It is important to identify factors that influence the performance of a biomarker in order to determine the optimal conditions for test performance. We propose a covariate-specific time-dependent positive predictive values curve to quantify the predictive accuracy of a prognostic marker measured on a continuous scale and with censored failure time outcome. The covariate effect is accommodated with a semiparametric regression model framework. In particular, we adopt a smoothed survival time regression technique (Dabrowska, 1997, The Annals of Statistics 25, 1510-1540) to account for the situation where risk for the disease occurrence and progression is likely to change over time. In addition, we provide asymptotic distribution theory and resampling-based procedures for making statistical inference on the covariate-specific positive predictive values. We illustrate our approach with numerical studies and a dataset from a prostate cancer study.
|
['Biomarkers, Tumor', 'Computer Simulation', 'Data Interpretation, Statistical', 'Diagnosis, Computer-Assisted', 'Humans', 'Male', 'Models, Statistical', 'Prevalence', 'Prognosis', 'Prostatic Neoplasms', 'Reproducibility of Results', 'Sensitivity and Specificity', 'Time Factors']
| 19,397,579
|
[['D23.101.140'], ['L01.224.160'], ['E05.245.380', 'E05.318.740.300', 'L01.313.500.750.190.380', 'N05.715.360.750.300', 'N06.850.520.830.300'], ['E01.158', 'L01.313.500.750.100.158'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500', 'E05.599.835', 'N05.715.360.750.530', 'N06.850.520.830.500'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E01.789'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['G01.910.857']]
|
['Chemicals and Drugs [D]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Primary hypoadrenocorticism in a dog receiving glucocorticoid supplementation.
|
A 5-year-old, spayed, female husky-Labrador retriever cross was diagnosed with primary hypoadrenocorticism, an uncommon endocrine disorder caused by a deficiency of glucocorticoid and mineralocorticoid hormones. Subtle clinical signs and previous treatment with exogenous glucocorticoid drugs required an adrenocorticotropic hormone stimulation test to confirm the diagnosis.
|
['Adrenal Insufficiency', 'Adrenocorticotropic Hormone', 'Animals', 'Dog Diseases', 'Dogs', 'Female', 'Glucocorticoids']
| 10,065,324
|
[['C19.053.500'], ['D06.472.699.327.935.531.500', 'D06.472.699.631.525.600.531.500', 'D12.644.400.400.935.531.500', 'D12.644.548.365.935.531.500', 'D12.644.548.691.525.690.531.500', 'D12.776.631.650.405.935.531.500'], ['B01.050'], ['C22.268'], ['B01.050.150.900.649.313.750.250.216.200'], ['D06.472.040.543', 'D27.505.696.399.472.488']]
|
['Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Application of liquid-liquid-liquid microextraction and ion-pair liquid chromatography coupled with photodiode array detection for the determination of chlorophenols in water.
|
A method termed as liquid-liquid-liquid microextraction was utilized to extract chlorophenols from water. The extracted chlorophenols, present in anionic form, were then separated, identified, and quantitated by ion-pair high-performance liquid chromatography with photodiode array detection (HPLC/DAD). For trace chlorophenol determination using HPLC/DAD, the chlorophenolate anion provides a better ultraviolet spectrum for quantitative and qualitative analyses than does uncharged chlorophenol. This is due to the auxochromic effect of the phenolate anion. In the study, experimental conditions such as organic phase identity, acceptor phase volume, sample agitation, extraction time, acceptor phase NaOH concentration, donor phase HCl concentration, salt addition, and UV absorption wavelength were optimized. Relative standard deviations (RSD, 2.3-5.4%), coefficients of determination (r2 0.9994-0.9999), and detection limits (0.049-0.081 ng mL(-1)) of the proposed method were investigated under the selected conditions. The method was successfully applied to analyses of reservoir and tap water samples, and the relative recoveries of chlorophenols from the spiked reservoir and tap water samples were 94.1-100.4% and 87.8-101.2%, respectively. The proposed method is capable of identifying and quantitating each analyte to 0.5 ng mL(-1), confirming the HPLC/DAD technique to be quite robust for monitoring trace levels of chlorophenols in water samples.
|
['Chlorophenols', 'Chromatography, Liquid', 'Reproducibility of Results', 'Spectrophotometry, Ultraviolet', 'Water Pollutants, Chemical']
| 18,420,216
|
[['D02.455.426.559.389.261.190', 'D02.455.426.559.389.657.190'], ['E05.196.181.400'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.196.712.726.802', 'E05.196.867.826.802'], ['D27.888.284.903.655']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Consecutive triplet pregnancies following in-vitro fertilization and embryo transfer. Two case reports.
|
The number of multiple pregnancies has been increasing as a result of the relatively widespread use of drugs for induction of ovulation and assisted conception techniques. This paper details the first reported cases of triplet pregnancies occurring in consecutive IVF cycles in two patients.
|
['Abortion, Spontaneous', 'Adult', 'Chorionic Gonadotropin', 'Clomiphene', 'Embryo Transfer', 'Female', 'Fertilization in Vitro', 'Follicle Stimulating Hormone', 'Humans', 'Menotropins', 'Pregnancy', 'Pregnancy, Ectopic', 'Triplets', 'Ultrasonography']
| 2,501,339
|
[['C13.703.039', 'G08.686.784.769.496.125'], ['M01.060.116'], ['D06.472.699.322.326', 'D06.472.699.649.367', 'D12.644.548.726.367', 'D12.776.780.400'], ['D02.455.426.559.389.150.700.125'], ['E02.875.800.500', 'E05.820.800.500'], ['E02.875.800.750', 'E05.820.800.750'], ['D06.472.699.322.576.288', 'D06.472.699.631.525.343.288', 'D12.644.548.691.525.343.288'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D06.472.699.322.576.583', 'D06.472.699.631.525.343.583', 'D12.644.548.691.525.343.583', 'D20.215.535'], ['G08.686.784.769'], ['C13.703.733'], ['M01.438.768'], ['E01.370.350.850']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Skin and peripheral lymph node invariant NKT cells are mainly retinoic acid receptor-related orphan receptor (gamma)t+ and respond preferentially under inflammatory conditions.
|
Lymph nodes (LNs) have been long considered as comprising few invariant NKT (iNKT) cells, and these cells have not been studied extensively. In this study, we unravel the existence of stable rather than transitional LN-resident NK1.1(-) iNKT cell populations. We found the one resident in peripheral LNs (PLNs) to comprise a major IL-17-producing population and to express the retinoic acid receptor-related orphan receptor (gamma)t (ROR(gamma)t). These cells respond to their ligand alpha-galactosylceramide (alpha-GalCer) in vivo by expanding dramatically in the presence of LPS, providing insight into how this rare population could have an impact in immune responses to infection. PLN-resident ROR(gamma)t(+) NK1.1(-) iNKT cells express concomitantly CCR6, the integrin alpha-chain alpha(E) (CD103), and IL-1R type I (CD121a), indicating that they might play a role in inflamed epithelia. Accordingly, skin epithelia comprise a major ROR(gamma)t(+) CCR6(+)CD103(+)CD121a(+) NK1.1(-) cell population, reflecting iNKT cell composition in PLNs. Importantly, both skin and draining PLN ROR(gamma)t(+) iNKT cells respond preferentially to inflammatory signals and independently of IL-6, indicating that they could play a nonredundant role during inflammation. Overall, our study indicates that ROR(gamma)t(+) iNKT cells could play a major role in the skin during immune responses to infection and autoimmunity.
|
['Animals', 'Cell Proliferation', 'Epithelial Cells', 'Galactosylceramides', 'Inflammation', 'Interleukin-17', 'Interleukin-6', 'Lymph Nodes', 'Mice', 'Mice, Knockout', 'Natural Killer T-Cells', 'Nuclear Receptor Subfamily 1, Group F, Member 3', 'Receptors, Retinoic Acid', 'Receptors, Thyroid Hormone', 'Skin']
| 19,587,013
|
[['B01.050'], ['G04.161.750', 'G07.345.249.410.750'], ['A11.436'], ['D02.065.313.250.450', 'D09.400.410.420.525.200.250.450', 'D10.390.470.675.200.250.450', 'D10.570.877.360.612.200.250.450'], ['C23.550.470'], ['D12.644.276.374.465.517', 'D12.776.467.374.465.517', 'D23.529.374.465.517'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['A10.549.400', 'A15.382.520.604.412'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['A11.118.637.555.567.569.290', 'A15.145.229.637.555.567.569.360', 'A15.382.490.555.567.569.470'], ['D12.776.260.643.182', 'D12.776.826.209.182'], ['D12.776.826.701', 'D12.776.930.775'], ['D12.776.624.664.700.830', 'D12.776.826.850'], ['A17.815']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cold crucible levitation melting of biomedical Ti-30 wt%Ta alloy.
|
Recently, titanium-tantalum alloys have been studied as implant materials for dental and orthopedic surgery. However, titanium and tantalum are difficult to mix by common arc melting and induction melting, because of their high melting point and the marked difference between their densities (Ti: 1,680 degrees C, 4.5 g/cm3, Ta: 2,990 degrees C, 16.6 g/cm3). Thus, the Cold Crucible Levitation Melting (CCLM) method was chosen to produce a Ti-30 wt%Ta binary alloy in the present study. The CCLM furnace, with 1 kg capacity, consisted of a water-cooled crucible comprising oxygen-free high purity copper segments and coils wrapped around the crucible and connected to a frequency inverter power supply. A qualified ingot of 1.0 kg of Ti-30 wt%Ta alloy was obtained. The ingot was characterized from the surface quality, chemical composition distribution and microstructure, and finally the melting process was discussed.
|
['Alloys', 'Biocompatible Materials', 'Chemistry, Physical', 'Copper', 'Dental Alloys', 'Electron Probe Microanalysis', 'Equipment Design', 'Hot Temperature', 'Humans', 'Materials Testing', 'Tantalum', 'Titanium', 'Water']
| 11,523,979
|
[['D01.552.033', 'D25.058', 'J01.637.051.058'], ['D25.130', 'D27.720.102.130', 'J01.637.051.130'], ['H01.181.529'], ['D01.268.556.195', 'D01.268.956.170', 'D01.552.544.195'], ['D25.339.208', 'J01.637.051.339.208'], ['E01.370.350.515.402.250', 'E05.196.867.800.360', 'E05.595.402.250', 'E05.799.830.360'], ['E05.320'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.570'], ['D01.268.556.837', 'D01.268.956.859', 'D01.552.544.837'], ['D01.268.557.800', 'D01.268.956.878', 'D01.552.547.800'], ['D01.045.250.875', 'D01.248.497.158.459.650', 'D01.650.550.925']]
|
['Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
|
Biliary intraductal papillary-mucinous neoplasm manifesting only as dilatation of the hepatic lobar or segmental bile ducts: imaging features in six patients.
|
OBJECTIVE: The purpose of this study was to evaluate the imaging features of intrahepatic biliary intraductal papillary-mucinous neoplasm manifesting only as dilatation of the lobar or segmental bile ducts without a visible mass to determine whether this type of cholangiocarcinoma can be recognized on the basis of distinct imaging features.CONCLUSION: Intrahepatic biliary intraductal papillary-mucinous neoplasm can spread along the mucosa without forming a mass and can produce a large amount of mucin. Severe dilatation of the lobar or segmental intrahepatic bile ducts with crowding and severe atrophy of the hepatic parenchyma are helpful imaging findings.
|
['Adenocarcinoma, Mucinous', 'Adenoma', 'Aged', 'Bile Duct Neoplasms', 'Diagnosis, Differential', 'Dilatation, Pathologic', 'Female', 'Humans', 'Liver Diseases', 'Male', 'Middle Aged', 'Tomography, X-Ray Computed']
| 18,716,109
|
[['C04.557.470.200.025.075', 'C04.557.470.590.075'], ['C04.557.470.035'], ['M01.060.116.100'], ['C04.588.274.120.250', 'C06.130.120.120', 'C06.130.320.120', 'C06.301.120.250'], ['E01.171'], ['C23.300.325'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C06.552'], ['M01.060.116.630'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
A case study evaluating deep inspiration breath-hold and intensity-modulated radiotherapy to minimise long-term toxicity in a young patient with bulky mediastinal Hodgkin lymphoma.
|
Radiotherapy plays an important role in the treatment of early-stage Hodgkin lymphoma, but late toxicities such as cardiovascular disease and second malignancy are a major concern. Our aim was to evaluate the potential of deep inspiration breath-hold (DIBH) and intensity-modulated radiotherapy (IMRT) to reduce cardiac dose from mediastinal radiotherapy. A 24 year-old male with early-stage bulky mediastinal Hodgkin lymphoma received involved-site radiotherapy as part of a combined modality programme. Simulation was performed in free breathing (FB) and DIBH. The target and organs at risk were contoured on both datasets. Free breathing-3D conformal (FB-3DCRT), DIBH-3DCRT, FB-IMRT and DIBH-IMRT were compared with respect to target coverage and doses to organs at risk. A 'butterfly' IMRT technique was used to minimise the low-dose bath. In our patient, both DIBH (regardless of mode of delivery) and IMRT (in both FB and DIBH) achieved reductions in mean heart dose. DIBH improved all lung parameters. IMRT reduced high dose (V20), but increased low dose (V5) to lung. DIBH-IMRT was chosen for treatment delivery. Advanced radiotherapy techniques have the potential to further optimise the therapeutic ratio in patients with mediastinal lymphoma. Benefits should be assessed on an individualised basis.
|
['Breath Holding', 'Hodgkin Disease', 'Humans', 'Male', 'Mediastinal Neoplasms', 'Organs at Risk', 'Radiotherapy Planning, Computer-Assisted', 'Radiotherapy, Intensity-Modulated', 'Time Factors', 'Young Adult']
| 28,188,697
|
[['G09.772.705.349'], ['C04.557.386.355', 'C15.604.515.569.355', 'C20.683.515.761.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.894.479', 'C08.846.187.580'], ['A01.635'], ['E02.950.825', 'L01.313.500.750.100.710.600.608'], ['E02.815.635.700.700', 'L01.313.500.750.100.710.600.550.700'], ['G01.910.857'], ['M01.060.116.815']]
|
['Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Named Groups [M]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
The Iron Chelator and Anticancer Agent Dp44mT Relieves Allergic Inflammation in Mice With Allergic Rhinitis.
|
Our previous study showed that an iron chelator and anticancer agent Di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT) has an antiinflammatory effect in human mast cells. However, antiinflammatory effect of Dp44mT remains unclear in animal models. In this study, we assessed whether administration of Dp44mT could relieve clinical symptoms of ovalbumin (OVA)-induced allergic rhinitis (AR) mice. After administration of Dp44mT, number of rubs was significantly decreased, and levels of histamine and IgE were suppressed in serum of AR mice. Also, serum levels of interleukin (IL)-1â, thymic stromal lymphopoietin (TSLP), and tumor necrosis factor (TNF)-á increased by OVA challenge were significantly lowered by administration of Dp44mT. T helper type 1 (Th1) cytokine interferon-ã level was significantly increased by administration of Dp44mT, whereas Th2 cytokines such as IL-4, IL-5, and IL-13 were significantly reduced by administration of Dp44mT. In intranasal tissues of AR mice, levels of IL-1â, TSLP, TNF-á, and IL-6 and activities and protein levels of caspase-1 were significantly reduced by administration of Dp44mT. Interestingly, administration of Dp44mT reduced number of infiltrated eosinophils and mast cells through the inhibition of macrophage inflammatory protein-2 and intercellular adhesion molecule-1 in intranasal tissues of AR mice. In conclusion, these results indicate that Dp44mT also has potential antiinflammatory effects in vivo as well as in vitro.
|
['Animals', 'Anti-Inflammatory Agents', 'Antineoplastic Agents', 'Chemokine CXCL2', 'Eosinophils', 'Inflammation', 'Intercellular Adhesion Molecule-1', 'Iron Chelating Agents', 'Mast Cells', 'Mice', 'Rhinitis, Allergic', 'Thiosemicarbazones']
| 29,967,928
|
[['B01.050'], ['D27.505.954.158'], ['D27.505.954.248'], ['D12.644.276.374.200.120.100', 'D12.644.276.374.200.600.940', 'D12.776.467.374.200.120.100', 'D12.776.467.374.200.600.940', 'D23.125.300.120.100', 'D23.125.300.600.940', 'D23.469.200.120.100', 'D23.469.200.600.940', 'D23.529.374.200.120.100', 'D23.529.374.200.600.940'], ['A11.118.637.415.345', 'A11.627.340.345', 'A15.145.229.637.415.345', 'A15.382.490.315.251'], ['C23.550.470'], ['D12.776.395.550.200.450', 'D12.776.543.550.200.450', 'D23.050.301.350.450'], ['D27.505.519.914.500.410', 'D27.720.832.500.410'], ['A11.329.427', 'A15.382.652'], ['B01.050.150.900.649.313.992.635.505.500'], ['C08.460.799.315', 'C08.674.453', 'C09.603.799.315', 'C20.543.480.680.443'], ['D02.845.746.703', 'D02.886.803']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Reduced allergic lung inflammation by root extracts from two species of Peucedanum through inhibition of Th2 cell activation.
|
ETHNOPHARMACOLOGICAL EVIDENCE: Peucedani Radix (PR), the root of Peucedanum praeruptorum Dunn (PPD) or Peucedanum decursivum (Miq.) Maxim. (PDM), has long been used in Korea to eliminate sputum, relieve cough, and reduce bronchus contraction. Furthermore, these therapeutic strategies are recognized as general and effective methods in western medicine as well as traditional Korean medicine.AIM OF THE STUDY: To determine and compare the anti-inflammatory effects of PPD extracts (PPDE) and PDM extracts (PDME) on allergic lung inflammation, using in vivo OVA-induced airway inflammation in mice and in vitro primary cell culture systems.MATERIALS AND METHODS: Eight-week-old female C57BL/6 mice were placed into four groups (n=4 per group): saline control, OVA-induced allergic lung inflammation with vehicle, or PPDE (200mg/kg) or PDME (200mg/kg) treatment. PR extracts (PRE) were administered from 1 week before 1st OVA sensitization to the day before sacrifice. Mice were sacrificed 18h after last OVA intra-nasal challenge followed by histological and biochemical analyses.RESULTS: Inflammatory phenotypes were alleviated with oral administration of PRE. PRE treatment decreased mucus production in airway epithelium, inflammatory cell number, eosinophilia, type 2 cytokines, and histamine in bronchoalveolar lavage fluid (BALF). Mice with PRE administration showed diminished activated CD4 T cell (CD4+CD25+ cell) and GATA-3 level in the lung. In addition, PRE treatment reduced Th2 cell activation in vitro, using Th2 polarization system.CONCLUSION: Our findings indicate that the anti-inflammatory effects of PRE arise from reduced Th2 cell activation and validate the clinical use of PR in traditional Korean medicine.
|
['Allergens', 'Animals', 'Anti-Asthmatic Agents', 'Apiaceae', 'Asthma', 'Bronchoalveolar Lavage Fluid', 'CD4-Positive T-Lymphocytes', 'Cell Count', 'Cytokines', 'Eosinophilia', 'Female', 'GATA3 Transcription Factor', 'Histamine', 'Immunoglobulin E', 'Lung', 'Mice, Inbred C57BL', 'Mucus', 'Ovalbumin', 'Plant Extracts', 'Plant Roots']
| 27,965,051
|
[['D23.050.063'], ['B01.050'], ['D27.505.954.796.050'], ['B01.650.940.800.575.912.250.075'], ['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['E05.927.100.500'], ['A11.118.637.555.567.569.200', 'A15.145.229.637.555.567.569.200', 'A15.382.490.555.567.569.200'], ['E01.370.225.500.195', 'E05.200.500.195', 'E05.242.195', 'G04.140'], ['D12.644.276.374', 'D12.776.467.374', 'D23.529.374'], ['C15.378.553.231'], ['D12.776.260.235.687', 'D12.776.260.257.300', 'D12.776.930.216.687', 'D12.776.930.314.300'], ['D02.092.211.215.501', 'D02.092.471.440', 'D03.383.129.308.373', 'D23.469.050.300'], ['D12.776.124.486.485.114.619.312', 'D12.776.124.790.651.114.619.312', 'D12.776.377.715.548.114.619.312'], ['A04.411'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['A12.200.503'], ['D12.644.861.557', 'D12.776.034.614', 'D12.776.256.159.157.663', 'D12.776.290.663', 'D12.776.872.557'], ['D20.215.784.500', 'D26.667'], ['A18.400']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Electrochemical aspects of pH electrodes.
|
When a pH electrode is applied intragastrically for long-term pH metry, problems may occur which are related to the specific conditions of gastric juice and to the possible difference between the temperature of buffer solutions and the body temperature. The fact that pH electrodes measure the activity of the hydrogen ion instead of its concentration may, in concentrated solutions (i.e. pH less than 2), lead to an error that is even compounded if other ions are present in high concentrations. This error normally does not influence comparisons between measurements obtained under the same conditions. It is, however, important to check which correction procedures are provided for the particular devices being used when comparing results obtained by different research groups. Calibration of the electrode at a temperature differing from body temperature may result in an important error. Complete correction for this error may be achieved for glass electrodes by a modified version of the Nernst equation; for monocrystalline antimony electrodes an empirically derived formula is available allowing for partial correction. In general, all types of electrodes should be calibrated using two different buffer solutions (one with a pH value between 6 and 8 and another with a pH of 2 or less) and the double-point interpolation method.
|
['Antimony', 'Body Temperature', 'Buffers', 'Calibration', 'Electrochemistry', 'Gastric Acidity Determination', 'Glass', 'Humans', 'Hydrogen-Ion Concentration', 'Microelectrodes', 'Monitoring, Physiologic']
| 2,225,514
|
[['D01.268.513.124', 'D01.268.556.050', 'D01.552.544.050'], ['E01.370.600.875.374', 'G07.110'], ['D27.720.470.280'], ['E05.978.155'], ['H01.181.529.307'], ['E01.370.225.124.300', 'E01.370.372.300', 'E05.200.124.300'], ['J01.637.437'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['E07.305.250.500'], ['E01.370.520']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
The potentiation of ototoxicity when aminooxyacetic acid and kanamycin are co-administered.
|
Aminooxyacetic acid (AOAA) has been shown to confer protection against noise-induced cochlear trauma [3]. We, therefore, decided to study the possible protective effect of AOAA against kanamycin (KM) ototoxicity and found, instead, that AOAA potentiated the toxicity. To produce ototoxicity in guinea pigs, KM is usually given in 10-14 daily doses of 400 mg/kg s.c. However, when combined with a single dose of AOAA (8, 11, 15, or 25 mg/kg) a single 400 mg/kg dose of KM is sufficient to cause cochlear damage. Such animals show a negative Preyer's reflex between 1 to 3 days post injection. 21 days later hearing thresholds as detected electrocochleographically at 2, 4, 8, 12 and 16 kHz have changed drastically sometimes to the point of being undetectable. The damage seen histologically at this time is destruction of both inner and outer hair cells. A pharmacokinetic analysis of this potentiation revealed a slight prolongation of KM's sojourn in the inner ear. The possible mechanisms of this unexpected, marked potentiation are discussed but remain unknown.
|
['Acetates', 'Aminooxyacetic Acid', 'Animals', 'Cochlea', 'Cochlear Microphonic Potentials', 'Drug Synergism', 'Guinea Pigs', 'Hair Cells, Auditory', 'Hearing Loss', 'Kanamycin', 'Time Factors']
| 6,490,543
|
[['D02.241.081.018', 'D10.251.400.045'], ['D02.092.570.050', 'D02.241.081.018.207'], ['B01.050'], ['A09.246.300.246'], ['G07.265.216.500.370.223', 'G07.888.250.223', 'G11.561.200.500.370.223'], ['G07.690.773.968.477'], ['B01.050.150.900.649.313.992.550'], ['A08.675.650.250', 'A08.675.650.915.750.600.350', 'A08.800.950.750.600.350', 'A09.246.300.246.577.325', 'A11.671.650.250', 'A11.671.650.915.750.600.350'], ['C09.218.458.341', 'C10.597.751.418.341', 'C23.888.592.763.393.341'], ['D09.408.051.476'], ['G01.910.857']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Possibilities for reconstruction in brachial plexus paralysis: neurotization].
|
Reinnervation by nerves other than the brachial plexus are necessary if avulsion of the roots prevents neural reconstruction of the brachial plexus itself, or if functional recovery is unsatisfactory and further reconstruction is necessary for improvement. The foreign nerves mostly used today for these neurotizations are the accessory nerve, the deep cervical plexus, the intercostal nerves and, more and more, the contralateral C7 spinal nerve. Our own experimental studies have demonstrated future concepts for reconstructions in very desperate cases to improve the final functional outcome in the useless extremity: cross-over reinnervation from the contralateral side, end-to-side nerve coaptation, and induction of sprouting by offering free muscle transplants to the regenerating nerve.
|
['Arm', 'Brachial Plexus', 'Humans', 'Muscle, Skeletal', 'Nerve Transfer', 'Paralysis', 'Prognosis']
| 9,931,677
|
[['A01.378.800.075'], ['A08.800.800.720.050'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.633.567', 'A10.690.552.500'], ['E04.525.550'], ['C10.597.622', 'C23.888.592.636'], ['E01.789']]
|
['Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Discordance and accordance between patient's and physician's assessments in rheumatoid arthritis.
|
OBJECTIVES: The American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) remission criteria for rheumatoid arthritis (RA) are more stringent than index-based criteria, making it more difficult to achieve a patient's global assessment (PGA) than an evaluator's global assessment (EGA). We investigated the reason for the discrepancy between the PGA and the EGA in a Japanese clinical cohort.METHOD: We assessed clinical and laboratory variables in our clinical cohort. The frequency of remission achievement according to the ACR/EULAR remission criteria and predictors of the discrepancy between the PGA and EGA were analysed.RESULTS: Of 370 patients with RA, 89 fulfilled PGA criteria and 167 patients fulfilled EGA criteria. The PGA was highly correlated with the visual analogue scale (VAS) pain score and non-inflammatory variables including Steinbrocker class and the Health Assessment Questionnaire Disability Index (HAQ-DI). Conversely, inflammatory variables, including swollen joint count (SJC), tender joint count (TJC), and C-reactive protein (CRP) levels, were significantly associated with the EGA. The main predictors of the discrepancy between the PGA and the EGA were patient's VAS pain score, SJC, and functional disability.CONCLUSIONS: Increased pain and functional disability led to a discrepancy towards a worse PGA than EGA, whereas increased SJC led to an accordance towards a worse EGA.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antirheumatic Agents', 'Arthritis, Rheumatoid', 'Disability Evaluation', 'Female', 'Humans', 'Male', 'Middle Aged', 'Patients', 'Physician-Patient Relations', 'Remission Induction', 'Rheumatology', 'Severity of Illness Index', 'Young Adult']
| 24,650,255
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D27.505.954.329'], ['C05.550.114.154', 'C05.799.114', 'C17.300.775.099', 'C20.111.199'], ['E01.370.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['M01.643'], ['F01.829.401.650.675', 'N05.300.660.625'], ['E02.860'], ['H02.403.429.730'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
|
Association between renal cysts and abdominal aortic aneurysm: A case-control study.
|
OBJECTIVE: To investigate the positive association between the presence of simple renal cysts (SRCs) and abdominal aortic aneurysm (AAA).METHOD: In a retrospective case-control study including subjects aged > 50 years, we evaluated the incidence of SRCs on computed tomography (CT) scan. We compared 91 consecutive patients with AAA referred from the Division of Vascular Surgery and 396 patients without AAA, randomly selected after being matched by age and gender from 3,186 consecutive patients who underwent abdominal CT. SRC was defined as a round or oval low-attenuation lesion with a thin wall and size > 4 mm on CT without obvious evidence of radiographic enhancement or septations. Patients were considered as having AAA if the size of aorta was greater than 3.0 cm.RESULTS: Patients with AAA and without AAA were similar in terms of age (67.9± 8.41 vs. 68.5±9.13 years) (p=0.889) and gender (71.4 vs. 71.2% of male subjects, respectively) (p=0.999). There was no difference in the prevalence of SRC between case and controls. Among individuals with AAA, 38 (41.8%; [95CI 32.5-52.6]) had renal cysts compared to 148 (37.4%; [95CI 32.7-42.2]) in the control group (p=0.473), with a prevalence ratio (PR) of 1.16 (95CI 0.80-1.68).CONCLUSION: We found no significant differences in the prevalence of SRCs among patients with AAA and controls. Our findings suggest that the presence of SRCs is not a risk factor or a marker for AAA.
|
['Aged', 'Aged, 80 and over', 'Aortic Aneurysm, Abdominal', 'Case-Control Studies', 'Female', 'Humans', 'Kidney Diseases, Cystic', 'Male', 'Middle Aged', 'Retrospective Studies', 'Tomography, X-Ray Computed']
| 28,977,104
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C14.907.055.239.075', 'C14.907.109.139.075'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.777.419.403', 'C13.351.968.419.403'], ['M01.060.116.630'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Identification, typing, and insecticidal activity of Xenorhabdus isolates from entomopathogenic nematodes in United Kingdom soil and characterization of the xpt toxin loci.
|
Xenorhabdus strains from entomopathogenic nematodes isolated from United Kingdom soils by using the insect bait entrapment method were characterized by partial sequencing of the 16S rRNA gene, four housekeeping genes (asd, ompR, recA, and serC) and the flagellin gene (fliC). Most strains (191/197) were found to have genes with greatest similarity to those of Xenorhabdus bovienii, and the remaining six strains had genes most similar to those of Xenorhabdus nematophila. Generally, 16S rRNA sequences and the sequence types based on housekeeping genes were in agreement, with a few notable exceptions. Statistical analysis implied that recombination had occurred at the serC locus and that moderate amounts of interallele recombination had also taken place. Surprisingly, the fliC locus contained a highly variable central region, even though insects lack an adaptive immune response, which is thought to drive flagellar variation in pathogens of higher organisms. All the X. nematophila strains exhibited a consistent pattern of insecticidal activity, and all contained the insecticidal toxin genes xptA1A2B1C1, which were present on a pathogenicity island (PAI). The PAIs were similar among the X. nematophila strains, except for partial deletions of a peptide synthetase gene and the presence of insertion sequences. Comparison of the PAI locus with that of X. bovienii suggested that the PAI integrated into the genome first and then acquired the xpt genes. The independent mobility of xpt genes was further supported by the presence of xpt genes in X. bovienii strain I73 on a type 2 transposon structure and by the variable patterns of insecticidal activity in X. bovienii isolates, even among closely related strains.
|
['Alleles', 'Animals', 'Bacterial Toxins', 'Base Sequence', 'DNA, Bacterial', 'Gene Dosage', 'Genes, Bacterial', 'Genetic Variation', 'Genomic Islands', 'Insecta', 'Insecticides', 'Nematoda', 'Phylogeny', 'RNA, Bacterial', 'RNA, Ribosomal, 16S', 'Recombination, Genetic', 'Soil Microbiology', 'Symbiosis', 'United Kingdom', 'Xenorhabdus']
| 16,957,209
|
[['G05.360.340.024.340.030'], ['B01.050'], ['D23.946.123'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D13.444.308.212'], ['G05.380.350'], ['G05.360.340.024.340.364.249', 'G05.360.340.358.024.249', 'G05.360.340.358.207.249'], ['G05.365'], ['G02.111.570.080.708.330.330', 'G05.360.080.708.330.330', 'G05.360.340.024.425.500'], ['B01.050.500.131.617'], ['D27.720.031.700.491', 'D27.888.723.491'], ['B01.050.500.500.294'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['D13.444.735.473'], ['D13.444.735.686.670'], ['G05.728'], ['H01.158.273.540.274.555', 'N06.850.425.300'], ['G06.550.800', 'G16.840'], ['Z01.542.363'], ['B03.440.450.425.890', 'B03.660.250.150.910']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Information Science [L]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
TLS/FUS-ERG fusion gene in acute lymphoblastic leukemia with t(16;21)(p11;q22) and monitoring of minimal residual disease.
|
This study reports a 1-year-old boy with precursor B cell acute lymphoblastic leukemia (ALL) carrying t(16;21)(p11;q22). Reverse transcriptase-polymerase chain reaction (RT-PCR) and direct sequence analysis showed TLS/FUS-ERG chimeric mRNA with a novel junctional pattern of exon 7 of TLS/FUS and exon 6 of ERG. He did not respond to ALL-oriented therapy. Complete remission (CR) was achieved by chemotherapy oriented for acute myeloid leukemia. Allogenic bone marrow transplantation was done and he has been in CR for 24 months. TLS/FUS-ERG chimeric mRNA was not detected after CR. This is the first report of an ALL patient with a TLS/FUS-ERG fusion transcript.
|
['Antineoplastic Combined Chemotherapy Protocols', 'Base Sequence', 'Chromosome Mapping', 'Chromosomes, Human, Pair 16', 'Chromosomes, Human, Pair 21', 'Cytarabine', 'Etoposide', 'Exons', 'Humans', 'Idarubicin', 'Infant', 'Male', 'Neoplasm, Residual', 'Oncogene Proteins, Fusion', 'Precursor Cell Lymphoblastic Leukemia-Lymphoma', 'RNA-Binding Protein FUS', 'Translocation, Genetic', 'Treatment Outcome']
| 16,263,589
|
[['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.183'], ['A11.284.187.520.300.415.420', 'G05.360.162.520.300.415.420'], ['A11.284.187.520.300.505.510', 'G05.360.162.520.300.505.510'], ['D03.383.742.680.245.453', 'D13.570.065.300', 'D13.570.685.245.453'], ['D02.455.426.559.847.638.960.675.250', 'D04.615.638.960.675.250', 'D09.408.348.275'], ['G05.360.340.024.340.137.232'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.455.426.559.847.562.050.200.300', 'D04.615.562.050.200.300', 'D09.408.051.059.200.300'], ['M01.060.703'], ['C04.697.700', 'C23.550.727.700'], ['D12.776.602.500.500', 'D12.776.624.664.500'], ['C04.557.337.428.600', 'C15.604.515.560.600', 'C20.683.515.528.600'], ['D12.776.157.725.813.750.905', 'D12.776.624.664.700.915', 'D12.776.664.962.813.750.902'], ['C23.550.210.870', 'G05.365.590.175.870', 'G05.558.860'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Named Groups [M]', 'Diseases [C]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Evaluation of sucromalt digestion in healthy children using breath hydrogen as a biomarker of carbohydrate malabsorption.
|
The measurement of hydrogen in exhaled breath is widely accepted as a non-invasive yet efficient means to evaluate carbohydrate malabsorption. Hydrogen is not normally produced by mammalian cells and its appearance in breath indicates incomplete small intestinal carbohydrate absorption with subsequent breakdown of the carbohydrate by anaerobic bacteria in the colon. This study was undertaken to evaluate the absorption of a novel, slowly digestible carbohydrate sweetener, sucromalt. Two experiments occurred approximately 2 weeks apart with the participants randomly consuming one of two test foods on each visit. Following baseline breath hydrogen measurements, healthy 8-10 year-old children (n = 10) consumed a yogurt breakfast containing either 15 g of inulin (positive control) or 30 g of sucromalt. Every 15 min during the next 6 h, samples of exhaled breath were taken from each participant for hydrogen content analysis, thereby establishing 24 total data points. Participants' 6 h breath hydrogen responses were plotted against their baseline measurement and appropriate statistical evaluations were applied to the data. Following ingestion of inulin, breath hydrogen stayed near baseline for approximately 2 h but rose rapidly thereafter to a steady state of 20-30 ppm, which continued to the end of the study period. In contrast, exhaled hydrogen following sucromalt ingestion remained at or near baseline for the entire 6 h test period. A significantly higher level of hydrogen was exhaled with inulin ingestion compared to sucromalt (incremental area under the curve, p = 0.002). Results indicated complete absorption of sucromalt's saccharide constituents in children.
|
['Biomarkers', 'Breath Tests', 'Child', 'Dietary Carbohydrates', 'Digestion', 'Disaccharides', 'Female', 'Fructose', 'Humans', 'Hydrogen', 'Intestinal Absorption', 'Malabsorption Syndromes', 'Male', 'Sweetening Agents']
| 22,166,954
|
[['D23.101'], ['E01.370.100'], ['M01.060.406'], ['D09.301', 'G07.203.300.362', 'J02.500.362'], ['G07.203.650.250', 'G10.261.190'], ['D09.698.629.305', 'D09.947.750'], ['D09.947.875.359.250', 'D09.947.875.465.354'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.268.406', 'D01.362.340'], ['G03.015.500.374.500', 'G03.787.024.500.374.500', 'G07.203.650.372.500', 'G07.690.725.015.500.374.500', 'G10.261.353.500'], ['C06.405.469.637', 'C18.452.603'], ['D27.720.372.300.353.609', 'G07.203.300.514.500.400.700', 'J02.500.514.500.400.700']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
|
Intracorporeal Traction of the Rectum with a Beaded Plastic Urinary Drainage Bag Hanger: Comparison with Conventional Laparoscopic Rectal Cancer Surgery.
|
BACKGROUND: Laparoscopic rectal cancer surgery with proper total mesorectal excision is a challenge for colorectal surgeons during trouble shooting. We used a beaded plastic urinary drainage bag hanger to encircle the rectum and clamp laparoscopic rectal transaction in this study.METHODS: Sixty-three patients with rectal cancer underwent laparoscopic radical rectal resection with curative intent between February 2015 and December 2015. Plastic beaded form urinary Foley catheter bag hanger was inserted intracorporeally via right lower 12-mm trocar, encircling the rectal tube distal to the rectal lesion followed by fastening. Thirty patients in the rectal resection group (28 laparoscopic, 2 robotic-assisted) using the commercial beaded plastic hanger for Foley catheter drainage were compared to 33 patients who underwent conventional laparoscopic rectal resection.RESULTS: Low anterior resection was performed for both groups. The Foley bag hanger group had less operation time (162.6 min vs. 187.3 min, p = 0.006) and fewer numbers of stapler cartilage (1.6 vs. 2.1, p = 0.001).CONCLUSIONS: Intracorporeal ligation of the rectum with a beaded plastic Foley catheter bag hanger could be used as a valuable method for rectal handling and transaction in laparoscopic rectal cancer surgery.
|
['Aged', 'Digestive System Surgical Procedures', 'Female', 'Humans', 'Laparoscopy', 'Male', 'Middle Aged', 'Operative Time', 'Plastics', 'Rectal Neoplasms', 'Rectum', 'Traction', 'Treatment Outcome', 'Urinary Catheterization']
| 28,748,421
|
[['M01.060.116.100'], ['E04.210'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.388.250.520', 'E04.502.250.520'], ['M01.060.116.630'], ['E04.614.374.500', 'N02.421.585.753.374.500'], ['D05.750.716', 'D25.720.716', 'J01.637.051.720.716'], ['C04.588.274.476.411.307.790', 'C06.301.371.411.307.790', 'C06.405.249.411.307.790', 'C06.405.469.491.307.790', 'C06.405.469.860.180.500'], ['A03.556.124.526.767', 'A03.556.249.249.767'], ['E04.555.720'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['E01.370.390.820', 'E02.148.947', 'E05.157.500']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
Conversion of 1-naphthol to naphthoquinone metabolites by rat liver microsomes: demonstration by high-performance liquid chromatography with reductive electrochemical detection.
|
1-Naphthol has recently been shown to be selectively toxic to short-term organ cultures of human colorectal tumor tissue. The mechanism underlying 1-naphthol's selective toxicity is as yet unknown, but may be due to the formation of naphthoquinone metabolites, which are known to be highly toxic to tumor cells. By using high-performance liquid chromatography with reductive electrochemical detection, it has been possible to show that 1-naphthol is converted to naphthoquinone metabolites by rat liver microsomes. At least two metabolic pathways, independent of cytochrome P-450, appear to be involved. Iron-dependent lipid peroxidation appears to be responsible for at least part of the conversion of 1-naphthol to predominantly 1,4-naphthoquinone, and it seems likely that superoxide anion radical generation by NADPH-cytochrome P-450 reductase could also catalyze this conversion. 1-Naphthol therefore seems to be converted to cytotoxic naphthoquinone metabolites by mechanism(s) dependent upon the generation of free radicals in rat liver microsomes. The results also demonstrate the utility of HPLC with reductive electrochemical detection for investigations of quinone metabolite formation and the measurement of quinones of both physiological and environmental interest.
|
['Animals', 'Chromatography, High Pressure Liquid', 'Cytochrome P-450 Enzyme System', 'Electrochemistry', 'In Vitro Techniques', 'Iron', 'Lipid Peroxides', 'Male', 'Microsomes, Liver', 'NADP', 'Naphthols', 'Naphthoquinones', 'Oxidation-Reduction', 'Rats', 'Rats, Inbred Strains']
| 6,517,596
|
[['B01.050'], ['E05.196.181.400.300'], ['D08.244.453', 'D08.811.682.690.708.170', 'D12.776.422.220.453'], ['H01.181.529.307'], ['E05.481'], ['D01.268.556.412', 'D01.268.956.287', 'D01.552.544.412'], ['D01.248.497.158.685.750.637', 'D01.339.431.374.637', 'D01.650.550.750.600', 'D02.389.338.450', 'D10.440'], ['A11.284.835.540.541'], ['D03.633.100.759.646.138.749', 'D08.211.625', 'D13.695.667.138.749', 'D13.695.827.068.749'], ['D02.455.426.559.847.638.638', 'D04.615.638.638'], ['D02.455.426.559.847.638.721', 'D02.806.550', 'D04.615.638.721'], ['G02.700', 'G03.295.531'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Deaf college students' reading comprehension and strategy use.
|
Two comprehension studies were conducted with 46 deaf college students. In the first, 20 deaf college students representing higher and lower reading-ability levels were tested for correctly stating the main idea of a passage, answering content questions, indicating their understanding of the words and phrases, and recognizing a topically incongruent sentence embedded in the passage. The results suggest that deaf students profess a better understanding of what they read than they are able to demonstrate. The students' inability to identify a topically incongruent sentence in the passage further suggests a need for them to more carefully and accurately evaluate their understanding of what they are reading. A second study investigated the effect of strategy review instruction on deaf college students' comprehension of short reading passages. Students reading at a higher level showed improved comprehension on the posttraining passage, but students reading at a lower level did not. Similarly, the control group of deaf students comparable to the higher-level readers did not show improved comprehension.
|
['Cognition', 'Deafness', 'Humans', 'Reading', 'Students', 'Universities']
| 11,865,569
|
[['F02.463.188'], ['C09.218.458.341.186', 'C10.597.751.418.341.186', 'C23.888.592.763.393.341.186'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.559.423.557'], ['M01.848'], ['I02.783.830', 'J03.832.830']]
|
['Psychiatry and Psychology [F]', 'Diseases [C]', 'Organisms [B]', 'Information Science [L]', 'Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 0
| 0
|
Interviews of female patients with borderline personality disorder who dropped out of group psychotherapy.
|
Premature termination from group psychotherapy continues to be a serious problem in the treatment of patients with borderline personality disorder (BPD). Qualitative research is regarded as an important means to shed light upon the complex dynamics leading to dropout. We conducted an interview study with patients having a diagnosis of BPD (n = 8) who dropped out from long-term group psychotherapy as a continuation therapy following intensive day treatment. The group therapists for these patients were interviewed as well (n = 12). The findings suggest the operation of many processes that contribute to dropout. Most significant appeared to be experiences of separation and loss of the day hospital that were not worked through and a failure of the group to regulate and contain the patients' affects. To integrate patients at risk of premature termination it seems necessary to pay attention to the strong negative emotions that they experience in the group. A higher treatment intensity than weekly group sessions may help to promote more beneficial group processes.
|
['Affect', 'Ambulatory Care', 'Borderline Personality Disorder', 'Day Care, Medical', 'Emotions', 'Female', 'Group Processes', 'Humans', 'Interview, Psychological', 'Motivation', 'Norway', 'Patient Care Planning', 'Patient Dropouts', 'Psychotherapy, Group', 'Risk Factors']
| 17,266,430
|
[['F01.470.047'], ['E02.760.106', 'N02.421.585.106'], ['F03.675.100'], ['E02.760.246', 'N02.421.585.246'], ['F01.470'], ['F01.829.316'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F04.669.599'], ['F01.658', 'F01.752.543.500.750'], ['Z01.542.816.374'], ['N04.590.233.624'], ['F01.100.150.750.500.610', 'F01.145.488.887.500.610', 'N05.300.150.800.500.610'], ['F04.754.864.581'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Formulation and evaluation of taste masked oral reconstitutable suspension of primaquine phosphate.
|
The purpose of this research was to mask the intensely bitter taste of primaquine phosphate (PRM) and to formulate suspension powder (cachets) of the taste masked drug. Taste masking was done using beta-cyclodextrin. To characterize and formulate taste masked cachets of PRM, the 1:25 M physical mixture was selected based on bitterness score. Phase solubility studies, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and X-ray powder diffraction (XRPD) were performed to identify the physicochemical interaction between drug and carrier, hence its effect on dissolution. Cachets were evaluated for angle of repose, sedimentation characterization and pH. In vitro drug release studies for physical mixture and kneaded system were performed at pH, 1.2 and 6.8. Bitterness score was evaluated using gustatory sensation test. Phase solubility studies showed weak interaction between PRM and CD. The FTIR, DSC and XRPD studies indicated inclusion complexation in physical mixture and kneaded system. In addition, kneaded system and physical mixture exhibited better drug release at pH 1.2 and negligible effect at pH 6.8. Cachets prepared using physical mixture, (DS24), showed complete bitter taste masking and easy redispersibility. Taste evaluation of cachets in human volunteers rated tasteless with a score of 0 to DS24 and 3 to DS25. Thus, results conclusively demonstrated successful taste masking and formulation of cachets with taste masked drug.
|
['Administration, Oral', 'Adult', 'Drug Evaluation, Preclinical', 'Female', 'Humans', 'Male', 'Pharmaceutical Solutions', 'Primaquine', 'Solubility', 'Taste Perception', 'Young Adult']
| 18,770,047
|
[['E02.319.267.100'], ['M01.060.116'], ['E05.290.750', 'E05.337.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D26.776.708', 'D27.505.954.578', 'D27.720.752'], ['D03.633.100.810.050.650'], ['G02.805'], ['F02.463.593.817'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[30 years of computer-based clinical documentation at the Heidelberg University Orthopedic Clinic. From basic documentation to medical controlling].
|
An overview of the 30 years history and development of documentation and information systems in the Orthopedic University Hospital Heidelberg is presented. Since the foundation in 1967 four developmental phases can be described: first initiatives of medical doctors, establishment of a basic documentation system for scientific purposes, strategic information system planning and realisation of information systems with the possibility of controlling in medical areas and thereby steering of the services. Planning and realisation were accomplished within the framework of the masterplans and concepts of the university clinics of the state of Baden-W?rttemberg.
|
['Academic Medical Centers', 'Documentation', 'Electronic Data Processing', 'Germany', 'History, 20th Century', 'Humans', 'Medical Records Systems, Computerized', 'Orthopedics']
| 10,326,202
|
[['N02.278.020'], ['L01.453.245'], ['L01.224.085'], ['Z01.542.315'], ['K01.400.504.968'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.940.968.625', 'L01.313.500.750.300.695', 'N04.452.859.564.650', 'N05.715.360.300.715.500.530', 'N06.850.520.308.940.968.625'], ['H02.403.810.494']]
|
['Health Care [N]', 'Information Science [L]', 'Geographicals [Z]', 'Humanities [K]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
|
Bethanechol and N-acetylcysteine mimic feeding signals and reverse insulin resistance in fasted and sucrose-induced diabetic rats.
|
Meal-induced insulin sensitization (MIS) is explained by the HISS (hepatic insulin sensitizing substance) hypothesis. In the presence of two "feeding signals," a pulse of insulin results in the release of HISS from the liver. HISS acts selectively on skeletal muscle and doubles the response to insulin. HISS is not released in the fasted state or in the sucrose-supplemented diabetes model. We tested the hypothesis that provision of both feeding signals allows insulin to cause HISS release in both the normal fasted and the diabetic model. The dynamic response to insulin (50 mU/kg over 5 min) was quantified using the rapid insulin sensitivity test (RIST). Gastric injection of a liquid test meal or i.v. administration of N-acetylcysteine in 24 h fasted rats raised hepatic glutathione to a similar degree (by 46%-47%). Hepatic denervation in fed rats eliminated the parasympathetic signal and eliminated MIS, and bethanechol completely restored MIS. Both compounds administered together allowed insulin to stimulate HISS release in 24 h fasted rats and in a diabetic model (9-week, 35% liquid sucrose supplement). Neither was effective alone. Both "feeding signals" are necessary and sufficient for insulin to stimulate HISS release.
|
['Acetylcysteine', 'Animals', 'Bethanechol', 'Biomimetic Materials', 'Diabetes Mellitus, Experimental', 'Fasting', 'Feeding Behavior', 'Glutathione', 'Insulin', 'Insulin Resistance', 'Liver', 'Male', 'Muscarinic Agonists', 'Muscle, Skeletal', 'Parasympathetic Nervous System', 'Rats', 'Rats, Sprague-Dawley', 'Sucrose']
| 21,326,345
|
[['D02.886.030.230.259', 'D12.125.166.230.259'], ['B01.050'], ['D02.092.877.883.088.100', 'D02.241.081.251.133.100', 'D02.675.276.148.100'], ['J01.637.087'], ['C18.452.394.750.074', 'C19.246.240', 'E05.598.500.374'], ['F01.145.407.400', 'G07.203.650.240.587', 'G07.203.650.353.400'], ['F01.145.113.547', 'F01.145.407', 'G07.203.650.353'], ['D12.644.456.448'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['C18.452.394.968.500', 'G07.690.773.984.617'], ['A03.620'], ['D27.505.519.625.120.140.500', 'D27.505.696.577.120.140.500'], ['A02.633.567', 'A10.690.552.500'], ['A08.800.050.600'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D09.698.629.305.770', 'D09.947.750.770']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Replicative deoxyribonucleic acid synthesis in isolated mitochondria from Saccharomyces cerevisiae.
|
The characteristics of a system for the in vitro synthesis of mitochondrial deoxyribonucleic acid (mtDNA) in mitochondria isolated from Saccharomyces cerevisiae are described. In this system the exclusive product of the reaction is mtDNA. Under optimal conditions the initial rate of synthesis is close to the calculated in vivo rate; the rate is approximately linear for 20 min but then decreases gradually with time. DNA synthesis proceeds for at least 60 min and the de novo synthesis of an amount of mtDNA equivalent to 15% of the mtDNA initially present is achieved. The rate and extent of synthesis observed with mitochondria isolated from grande and petite (rho(-)) strains were similar. The mode of DNA synthesis is semiconservative; after density labeling with 5-bromodeoxyuridine triphosphate, in vitro, the majority of labeled DNA fragments of duplex molecular weight, 6 x 10(6), are of a density close to that calculated for hybrid yeast mtDNA. The density label is incorporated into one strand of the duplex molecules. These properties indicate that the synthesis resembles replicative rather than repair synthesis. This system therefore provides a convenient method for the study of mtDNA synthesis in S. cerevisiae. The observation that mtDNA synthesis is semiconservative in vitro suggests that the dispersive mode of synthesis observed in S. cerevisiae in vivo labeling studies is the result of some other process, possibly a high recombination rate.
|
['Adenosine Triphosphate', 'Centrifugation, Density Gradient', 'DNA Repair', 'DNA Replication', 'DNA, Mitochondrial', 'DNA, Single-Stranded', 'Hot Temperature', 'In Vitro Techniques', 'Kinetics', 'Magnesium', 'Mitochondria', 'Molecular Weight', 'Mutation', 'Saccharomyces cerevisiae', 'Thymine Nucleotides']
| 324,990
|
[['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['E05.181.724.336', 'E05.196.941.336'], ['G02.111.222', 'G05.219'], ['G02.111.225', 'G05.226'], ['D13.444.308.283.225'], ['D13.444.308.497', 'G02.111.570.820.486.437', 'G05.360.580.437'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['E05.481'], ['G01.374.661', 'G02.111.490'], ['D01.268.552.437', 'D01.268.557.500', 'D01.552.547.500'], ['A11.284.430.214.190.875.564', 'A11.284.835.626'], ['G02.494'], ['G05.365.590'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D03.383.742.686.706', 'D13.695.201.789', 'D13.695.740.706']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Pharmacophore modeling, 3D-QSAR and DFT studies of IWR small-molecule inhibitors of Wnt response.
|
In this study, a 5-point pharmacophore model was developed and the model was used to generate a predictive atom-based 3D quantitative structure activity relationship (3D-QSAR) analysis for the studied dataset of 50 compounds. The obtained 3D-QSAR model shows correlation coefficient (R(2)) of 0.87 for training set compounds and excellent predictive power (Q(2)) of 0.81 for cross-validated test set compounds. External validation indicated that our 3D-QSAR model has high predictive power with [Formula: see text] and [Formula: see text] values of 0.99 and 0.65, respectively. The most active and least active compounds were further optimized using density functional theory at B3LYP/3-21*G level. Further, pharmacophoric model was employed for pharmacophore-based screening to identify potential inhibitors against Wnt/â-catenin pathway. Hence, these molecules could act as selective inhibitors of Wnt/â-catenin pathway which can be experimentally validated. The backbone of these inhibitors could serve as templates for designing drug-like molecules specifically targeting Wnt/â-catenin pathway.
|
['Drug Design', 'Humans', 'Hydrogen Bonding', 'Models, Molecular', 'Molecular Structure', 'Quantitative Structure-Activity Relationship', 'Small Molecule Libraries', 'Wnt Proteins', 'Wnt Signaling Pathway', 'beta Catenin']
| 23,914,783
|
[['E05.290.500', 'H01.158.703.007.338.500', 'H01.181.466.338.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.282'], ['E05.599.595'], ['G02.111.570', 'G02.466'], ['G02.111.830.500', 'G07.690.773.997.500'], ['D27.720.470.765'], ['D12.776.467.984', 'D23.529.984'], ['G02.111.820.925', 'G04.835.925'], ['D12.776.091.249', 'D12.776.220.145.500', 'D12.776.930.130']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Transvaginal cholecystectomy learning curve.
|
BACKGROUND: There are few surgeons in the United States, within private practice and academic centers, currently performing transvaginal cholecystectomies (TVC). The lack of exposure to TVC during residency or fellowship training, coupled with a poorly defined learning curve, further limits interested surgeons who want to apply this technique to their practice. This study describes the learning curve encountered during the introduction of TVC to our academic facility.METHODS: This study is an analysis of consecutive TVCs performed between August 14, 2009 and August 3, 2012 at an academic center. The TVC patients were divided into sequential quartiles (n = 15/16). The learning curve outcome was measured as the operative time of TVC patients and compared to the operative time of female laparoscopic cholecystectomy (LC) patients performed during the same time period.RESULTS: Sixty-one patients underwent a TVC with a mean age of 38 ± 12 years and mean BMI was 29 ± 6 kg/m(2). Sixty-seven female patients who underwent a LC with average age 41 ± 15 years and average BMI 33 ± 12 kg/m(2). The average operative time of LC patients and TVC patients was 48 ± 20 and 60 ± 17 min, respectively. Significant improvement in TVC operative times was seen between the first (n = 15 TVCs) and second quartiles (p = 0.04) and stayed relatively constant for third quartile, during which there was no statistically significant difference between the mean LC operative time for the second and third TVC quartilesCONCLUSIONS: The learning curve of a fellowship-trained surgeon introducing TVC to their surgical repertoire, as measured by improved operative times, can be achieved with approximately 15 cases.
|
['Adult', 'Cholecystectomy', 'Cholecystectomy, Laparoscopic', 'Female', 'Humans', 'Learning Curve', 'Operative Time']
| 25,294,548
|
[['M01.060.116'], ['E04.210.120.172'], ['E04.210.120.172.140', 'E04.502.250.520.160'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.784.629.529.274'], ['E04.614.374.500', 'N02.421.585.753.374.500']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Topical application of a corticosteroid destabilizes the host-parasite relationship in an experimental model of the oral carrier state of Candida albicans.
|
Using an experimental model in the mouse we have shown that both local and central lines of defense, involving CD4+ T cells, participate in a dynamic interaction to maintain a long-term carrier state of Candida albicans in the oral cavity. We have tested the impact of a predisposing factor to oral candidiasis in the form of a topical application of a corticosteroid (Topsyn gel) to the oral mucosa for 75 mice twice a day for a 20-day period. Very rapidly after the treatment was initiated, i.e. on day 4, the residual population of Candida increased up to 40-fold and by day 21, the population was 400-fold that of the carrier state. The resident population of intraepithelial CD4+ T cells in the oral mucosa virtually disappeared during the treatment. A topical corticosteroid application also resulted in a massive depletion of T cells in the lymph nodes and in the transient abrogation of the DTH reaction to Candida antigens. On cessation of treatment, normal levels of both Candida and intraepithelial CD4+ T cells were also quickly restored. These results suggest that resistance to superficial invasion by Candida is linked to the presence of an oral mucosal line of defense and that topical application of corticosteroids may dramatically shift the host-parasite relationship in favor of Candida.
|
['Administration, Topical', 'Animals', 'CD4-Positive T-Lymphocytes', 'Candidiasis, Oral', 'Carrier State', 'Dose-Response Relationship, Drug', 'Fluocinonide', 'Host-Parasite Interactions', 'Hypersensitivity, Delayed', 'Lymph Nodes', 'Lymphocyte Activation', 'Macrophage-1 Antigen', 'Male', 'Mice', 'Mice, Inbred DBA', 'Mouth Mucosa', 'Spleen', 'T-Lymphocyte Subsets']
| 7,599,602
|
[['E02.319.267.120'], ['B01.050'], ['A11.118.637.555.567.569.200', 'A15.145.229.637.555.567.569.200', 'A15.382.490.555.567.569.200'], ['C01.150.703.160.180', 'C07.465.130'], ['N06.850.520.169'], ['G07.690.773.875', 'G07.690.936.500'], ['D04.210.500.745.432.370.325', 'D04.210.500.908.394.300'], ['G16.527.200.400'], ['C20.543.418'], ['A10.549.400', 'A15.382.520.604.412'], ['E01.370.225.812.482', 'E05.200.812.482', 'E05.478.594.530', 'G12.450.050.400.545', 'G12.565'], ['D12.776.543.750.705.408.495.500', 'D12.776.543.750.705.408.600.500', 'D12.776.543.750.705.833.500'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.500', 'B01.050.150.900.649.313.992.635.505.500.400.500'], ['A10.615.550.599', 'A14.549.512'], ['A10.549.700', 'A15.382.520.604.700'], ['A11.118.637.555.567.550.500', 'A11.118.637.555.567.569.500', 'A15.145.229.637.555.567.550.500', 'A15.145.229.637.555.567.569.500', 'A15.382.490.555.567.550.500', 'A15.382.490.555.567.569.500']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Zinc deficiency in two infants during total parenteral alimentation for diarrhea.
|
Zinc deficiency was observed in two infants receiving total parenteral alimentation for chronic diarrhea. An acrodermatitis enteropathica-like rash occurred in both of the infants. Staphylococcus aureus was cultured from the rash. Treatment with zinc resulted in rapid cure of the rash and a subsidence of other signs consistent with zinc deficiency.
|
['Acrodermatitis', 'Child, Preschool', 'Copper', 'Deficiency Diseases', 'Diarrhea, Infantile', 'Humans', 'Infant', 'Japan', 'Male', 'Parenteral Nutrition', 'Parenteral Nutrition, Total', 'Staphylococcal Infections', 'Staphylococcus aureus', 'Zinc']
| 814,805
|
[['C16.131.831.066', 'C17.800.174.100', 'C17.800.804.066'], ['M01.060.406.448'], ['D01.268.556.195', 'D01.268.956.170', 'D01.552.544.195'], ['C18.654.521.500'], ['C23.888.821.214.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['Z01.252.474.463', 'Z01.639.595'], ['E02.421.505', 'E02.642.500.505'], ['E02.421.505.575', 'E02.642.500.505.750'], ['C01.150.252.410.868'], ['B03.300.390.400.800.750.100', 'B03.353.500.750.750.100', 'B03.510.100.750.750.100', 'B03.510.400.790.750.100'], ['D01.268.556.940', 'D01.268.956.906', 'D01.552.544.940']]
|
['Diseases [C]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
|
Risk perception and concern among brothers of men with prostate carcinoma.
|
BACKGROUND: It is important for clinicians, researchers, and others who shape public health policy to understand the demographic correlates and psychologic factors that drive health behaviors, such as screening for early detection of cancer, particularly among individuals at high risk for developing the disease.METHODS: One-hundred eleven men whose brothers were diagnosed with prostate carcinoma completed a computer-assisted telephone interview aimed to assess their perception of absolute risk and concern about developing prostate carcinoma over the next 10 years and across their lifetime. Comparisons were made between selected demographic, behavioral, family pedigree characteristics, and measures of perceived risk and concern.RESULTS: The majority of men perceived their personal risk of developing prostate carcinoma to be > or =50%. Men who at the time of the interview were younger than their affected brother were significantly more concerned about prostate carcinoma and perceived their risk to be higher than men who were older than their brother. Estimates of personal risk and concern were also uniformly higher among men with more than one first-degree relative affected with prostate carcinoma compared to men with only one affected first-degree relative. Risk perception and concern about an impending prostate carcinoma diagnosis were associated with the use of supplements marketed for prostate health.CONCLUSIONS: The findings indicated that birth order in relation to a brother diagnosed with prostate carcinoma is significantly associated with risk perception and concern in unaffected family members. These results highlight the need for further study of the familial dynamics and characteristics that drive health behaviors and stress importance of public health education to inform men of personal risk assessment as well as the risks and benefits of screening. These studies ultimately can contribute to the success of strategies for the primary prevention and early detection of cancer.
|
['Adult', 'Aged', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Male', 'Middle Aged', 'Prostatic Neoplasms', 'Risk Assessment', 'Siblings', 'Surveys and Questionnaires']
| 15,042,690
|
[['M01.060.116'], ['M01.060.116.100'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750'], ['E05.318.740.600.800.715', 'N04.452.871.715', 'N05.715.360.750.625.700.690', 'N06.850.505.715', 'N06.850.520.830.600.800.715'], ['F01.829.263.500.490', 'I01.880.853.150.500.505', 'M01.781'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
All-case post-marketing surveillance of 1371 patients treated with pirfenidone for idiopathic pulmonary fibrosis.
|
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic fibrotic lung disease with a median survival of 2-5 years and limited treatment options. In 2008, pirfenidone became the first drug to be approved for IPF treatment in Japan. The aim of this study was to assess the safety of pirfenidone for IPF treatment in a clinical setting.METHODS: We conducted a safety-oriented post-marketing surveillance of all patients with IPF who were administered pirfenidone in the first year after its launch in Japan. This was a prospective, non-interventional, observational study. Case report forms were used to collect survey data, comprising adverse events, acute exacerbations, patient demographics, concomitant drug use, and concurrent treatment data.RESULTS: Of the 1371 patients available for safety evaluation, two-thirds had stage III-IV disease. The incidence of total adverse drug reactions (ADRs) was 64.6%. ADRs with an incidence of ?3% were decreased appetite, photosensitivity reaction, nausea, abdominal discomfort, malaise, somnolence, and hepatic function abnormal. This safety profile was consistent with the findings in phase II and III trials in Japan. The discontinuation rates due to adverse events at 12 months for each disease stage were similar; however, discontinuation caused by disease progression increased with disease severity. Treatment with pirfenidone stabilized both vital capacity and subjective symptoms in most patients (70-80%) treated for at least 6 months.CONCLUSIONS: This post-marketing surveillance in Japan showed that pirfenidone was generally well tolerated in patients with IPF, including those with severe lung function impairment.
|
['Aged', 'Disease Progression', 'Female', 'Humans', 'Idiopathic Pulmonary Fibrosis', 'Male', 'Middle Aged', 'Product Surveillance, Postmarketing', 'Prospective Studies', 'Pyridones', 'Severity of Illness Index', 'Treatment Outcome', 'Vital Capacity']
| 26,344,613
|
[['M01.060.116.100'], ['C23.550.291.656'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C08.381.765.500'], ['M01.060.116.630'], ['E05.337.800'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['D03.383.725.791'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['E01.370.386.700.485.750.900', 'G09.772.850.970']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III.
|
Escherichia coli contains two type 1 topoisomerases, topoisomerase I and III. Although topoisomerase III can be purified as a potent decatenase, its role in DNA metabolism is unclear. In order to address this issue, the gene encoding topoisomerase III from E. coli has been molecularly cloned and its DNA sequence determined. The cloned fragment of DNA contains an open reading frame that can encode a polypeptide of 73.2 kDa. The first 20 amino acids of this open reading frame are identical to those of topoisomerase III as determined by amino-terminal gas-phase microsequencing. Expression of the polypeptide encoded by this open reading frame, using a bacteriophage T7 transient expression system, results in the accumulation of a 74-kDa polypeptide. Soluble extracts prepared from cells overexpressing this gene product show a dramatic increase in topoisomerase activity when compared with control extracts. We propose that this gene be designated topB.
|
['Amino Acid Sequence', 'Base Sequence', 'Cloning, Molecular', 'Codon', 'DNA Topoisomerases, Type I', 'DNA, Bacterial', 'Escherichia coli', 'Genes, Bacterial', 'Molecular Sequence Data', 'Restriction Mapping', 'Sequence Homology, Nucleic Acid']
| 2,553,698
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.220'], ['D13.444.735.544.355', 'G05.360.335.355', 'G05.360.340.024.340.137.190'], ['D08.811.399.403.483', 'D12.776.157.687.375', 'D12.776.660.720.375'], ['D13.444.308.212'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['G05.360.340.024.340.364.249', 'G05.360.340.358.024.249', 'G05.360.340.358.207.249'], ['L01.453.245.667'], ['E05.393.183.620.650', 'E05.393.712'], ['G02.111.810.550', 'G05.810.550']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Regulation of the c-fos promoter by the ternary complex factor Sap-1a and its coactivator CBP.
|
The c-fos proto-oncogene is activated by a plethora of signals via the transcription factors Sap-1a and CREB. Recently, the coactivator CBP has been demonstrated to act in concert with CREB when CREB is phosphorylated by protein kinase A. We show that CBP also binds directly to Sap-1a. While phosphorylation of Sap-1a by mitogen-activated protein kinases is not necessary for CBP/Sap-1a interaction, functional cooperation between these two proteins requires Sap-1a to become phosphorylated. CBP-antagonists impair Sap-1a-mediated transactivation. Similarly, the CBP antagonist E1A suppresses c-fos upregulation by phosphorylated CREB, indicating that CBP is a central component of c-fos regulation. Furthermore, CBP is phosphorylated by protein kinase A in vitro and the transactivation potential of the carboxy-terminal region of CBP is enhanced in the presence of active protein kinase A in vivo. Thus, CBP, in addition to CREB, is a target for cAMP-dependent signaling. However, combined phosphorylation of CBP by protein kinase A and mitogen-activated protein kinases appears to be non-cooperative, suggesting that CBP serves the function of a dampening integrator of two different signaling pathways.
|
['Animals', 'Cell Line', 'Cyclic AMP Response Element-Binding Protein', 'Cyclic AMP-Dependent Protein Kinases', 'Genes, fos', 'Phosphorylation', 'Promoter Regions, Genetic', 'Protein Binding', 'Rabbits', 'Trans-Activators']
| 8,649,857
|
[['B01.050'], ['A11.251.210'], ['D12.776.260.108.184', 'D12.776.930.127.184'], ['D08.811.913.696.620.682.700.150.125', 'D12.644.360.200.125', 'D12.776.476.200.125'], ['G05.360.340.024.340.375.500.791.330'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['G02.111.679', 'G03.808'], ['B01.050.150.900.649.313.968.700'], ['D12.776.260.755', 'D12.776.930.900', 'D12.776.964.925.984']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Comparison of threshold estimation in infants with hearing loss or normal hearing using auditory steady-state response evoked by narrow band CE-chirps and auditory brainstem response evoked by tone pips.
|
OBJECTIVE: The objective of this study is to compare air-conduction thresholds obtained with ASSR evoked by narrow band (NB) CE-chirps and ABR evoked by tone pips (tpABR) in infants with various degrees of hearing loss.DESIGN: Thresholds were measured at 500, 1000, 2000 and 4000 Hz. Data on each participant were collected at the same day.STUDY SAMPLE: Sixty-seven infants aged 4 d to 22 months (median age = 96 days), resulting in 57, 52, 87 and 56 ears for 500, 1000, 2000 and 4000 Hz, respectively.RESULTS: Statistical analysis was performed for ears with hearing loss (HL) and showed a very strong correlation between tpABR and ASSR evoked by NB CE-chirps: 0.90 (n = 28), 0.90 (n = 28), 0.96 (n = 42) and 0.95 (n = 30) for 500, 1000, 2000 and 4000 Hz, respectively. At these frequencies, the mean difference between tpABR and ASSR was -3.6 dB (± 7.0), -5.2 dB (± 7.3), -3.9 dB (± 5.2) and -5.2 dB (± 4.7). Linear regression analysis indicated that the relationship was not influenced by the degree of hearing loss.CONCLUSION: We propose that dB nHL to dB eHL correction values for ASSR evoked by NB CE-chirps should be 5 dB lower than values used for tpABR.
|
['Acoustic Stimulation', 'Audiometry', 'Auditory Perception', 'Bone Conduction', 'Case-Control Studies', 'Evoked Potentials, Auditory, Brain Stem', 'Hearing', 'Hearing Loss', 'Humans', 'Infant', 'Infant, Newborn', 'Linear Models', 'Neonatal Screening', 'Predictive Value of Tests', 'Reproducibility of Results', 'Severity of Illness Index']
| 27,715,342
|
[['E02.037', 'E02.190.888.030', 'E05.723.136'], ['E01.370.382.375.060'], ['F02.463.593.071', 'G07.888.125'], ['F02.830.816.263.500', 'G07.888.500.500', 'G11.561.790.263.398'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['G07.265.216.500.370.300', 'G07.888.250.300', 'G11.561.200.500.370.300'], ['F02.830.816.263', 'G07.888.500', 'G11.561.790.263'], ['C09.218.458.341', 'C10.597.751.418.341', 'C23.888.592.763.393.341'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['M01.060.703.520'], ['E05.318.740.500.500', 'E05.318.740.750.425', 'E05.599.835.750', 'N05.715.360.750.530.460', 'N05.715.360.750.695.460', 'N06.850.520.830.500.500', 'N06.850.520.830.750.425'], ['E01.370.225.910', 'E01.370.500.580', 'E05.200.910', 'E05.318.308.980.438.580.580', 'N02.421.726.233.443.816', 'N05.715.360.300.800.438.500.575', 'N06.850.520.308.980.438.580.580', 'N06.850.780.500.580'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
In vitro activities of semisynthetic pneumocandin L-733,560 against fluconazole-resistant and -susceptible Candida albicans isolates.
|
Lipopeptide L-733,560 is a water-soluble derivative of pneumocandin B0 that exhibits enhanced anti-Candida activity. We investigated the in vitro activity of L-733,560 compared with those of amphotericin B, flucytosine, and itraconazole, against fluconazole-resistant (n = 44) and fluconazole-susceptible (n = 46) Candida albicans isolates. Tests were performed with a photometer-read broth microdilution method with RPMI-2% glucose and National Committee for Clinical Laboratory Standards reference strains. Except for those of itraconazole, MICs were not significantly different between the two groups of isolates, as expected for agents with different mechanisms of action. L-733,560 was the most active agent against C.albicans, with MICs for 50 and 90% of the strains tested of 0.01 and 0.06 microgram/ml, respectively.
|
['Amphotericin B', 'Anti-Bacterial Agents', 'Antifungal Agents', 'Candida albicans', 'Drug Resistance, Microbial', 'Fluconazole', 'Flucytosine', 'Itraconazole', 'Microbial Sensitivity Tests', 'Peptides']
| 8,723,483
|
[['D02.540.576.500.500'], ['D27.505.954.122.085'], ['D27.505.954.122.136'], ['B01.300.107.795.095.326', 'B01.300.381.147.326', 'B01.300.930.176.326'], ['G06.225', 'G07.690.773.984.269'], ['D03.383.129.799.450'], ['D03.383.742.698.421.431'], ['D03.383.129.799.550', 'D03.383.606.530'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['D12.644']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Evidence that associative interactions between synapses during the induction of long-term potentiation occur within local dendritic domains.
|
The present study evaluates whether the associative interactions between synapses that lead to long-term potentiation and depression (LTP and LTD) can occur between spatially segregated synapses of the medial and lateral temporodentate pathway of the rat. Coconditioning of crossed and ipsilateral pathways resulted in LTP of the crossed system only when the current sinks of the two conditioned pathways overlapped sufficiently. Likewise, conditioning of an ipsilateral pathway alone resulted in LTD of the crossed pathway only when those current sinks overlapped sufficiently. These observations support the idea that associative events that lead to LTP or LTD can be restricted to a local dendritic domain. The postsynaptic cell can therefore serve as more than one unit of integration for synaptic modification.
|
['Animals', 'Dendrites', 'Evoked Potentials', 'Models, Neurological', 'Rats', 'Synapses']
| 3,353,384
|
[['B01.050'], ['A08.675.256', 'A11.284.180.225', 'A11.671.240'], ['G07.265.216.500', 'G11.561.200.500'], ['E05.599.395.642'], ['B01.050.150.900.649.313.992.635.505.700'], ['A08.850', 'A11.284.149.165.420.780']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The effect of a creative art program on self-esteem, hope, perceived social support, and self-efficacy in individuals with multiple sclerosis: a pilot study.
|
BACKGROUND: Creative art has been found to be beneficial to some patients with chronic illness. Little is understood about how creative art can benefit individuals living with multiple sclerosis (MS).OBJECTIVES: The purpose of the pilot study was to determine if there was a difference in self-esteem, hope, perceived social support, and self-efficacy in individuals with MS after a 4-week creative art program.METHODS: A one-group, pretest/posttest design was used. The convenience sample of 14 individuals was recruited from MS Centers and the National MS Society. They ranged in age from 29 to 70 years (M = 51.3 years, SD = 12.5 years). Participants included 14 women. The creative art program included week 1-watercolor, week 2-collage making, week 3-beading, and week 4-knitting. Each of the four weekly sessions was facilitated by a registered nurse with expertise in MS and lasted 2 hours. Creative artists instructed participants and provided a hands-on experience for each of the creative projects. Participants were free to share thoughts, experiences, and words of support and encouragement during each session. The variables were measured before starting the creative art program and after the final session. The instruments included the Rosenberg Self-Esteem Scale, the Herth Hope Index, the Modified Social Support Survey, the MS Self-Efficacy Scale, and a sociodemographic questionnaire. The Statistical Package for the Social Sciences Version 16.0 was used to analyze the data.RESULTS: There was a significant increase in all variables after the creative art program as follows: self-esteem (t = -3.05, p = 009), hope (t = -3.96, p = .002), social support (t = -2.21, p = .046), self-efficacy to function with MS (t = -2.68, p = .019), and self-efficacy to control MS (t = 3.22, p = .007). The power analysis revealed a large effect size for hope (d = 1.06), self-esteem (d = 0.82), and self-efficacy (control; d = 0.86). A medium effect size was found for self-efficacy (function; d = 0.72) and social support (d = 0.59).CONCLUSIONS: The creative art program was found to be effective and had a positive influence on self-esteem, hope, social support, and self-efficacy to function and control MS. Creative art has the potential to enhance the lives of those living with MS and should be investigated with a larger sample of participants.
|
['Adult', 'Art Therapy', 'Female', 'Hope', 'Humans', 'Middle Aged', 'Missouri', 'Multiple Sclerosis', 'Pilot Projects', 'Self Efficacy', 'Social Perception', 'Social Support', 'Surveys and Questionnaires']
| 25,285,594
|
[['M01.060.116'], ['E02.190.888.124', 'E02.760.169.063.500.100', 'E02.831.100', 'F04.754.070'], ['F01.470.572'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['Z01.107.567.875.510.515'], ['C10.114.375.500', 'C10.314.350.500', 'C20.111.258.250.500'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['F01.752.747.792.700'], ['F02.463.593.752'], ['I01.880.853.500.600'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Geographicals [Z]', 'Diseases [C]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
Bipolar sensation seeking is associated with a propensity to abuse rather than to temperamental characteristics.
|
OBJECTIVE: As some temperament profiles may be markers of genetic vulnerability traits, we aimed to compare sensation seeking in euthymic bipolar patients and in controls.METHODS: One hundred ninety-four patients fulfilling DSM-IV diagnostic criteria for bipolar disorders (BP), 81% of whom presented type I BP, and 95 controls were included in this study. Euthymia was assessed using both the MADRS and Bech mania scales. Subjects were evaluated using the French abbreviated form of Zuckerman's Sensation Seeking Scale (SSS), which provide a total score (TS) and four subscores: Thrill and Adventure Seeking (TAS), Experience Seeking (ES), Disinhibition (Dis), and Boredom Susceptibility (BS).RESULTS: SSS total score differed significantly between men (17.2 +/- 0.5) and women (15.3 +/- 0.6) (P = 0.02) and all the subscores were negatively correlated with age. On adjustment for sex and age, we found that bipolar patients had a high Dis score (P = 0.003). However, if the same analysis was performed with a lifetime history of alcohol abuse or dependence as a covariable, no such difference was found (P = 0.436). The SSS demonstrated a high degree of test-retest reliability (ICC = 0.91).CONCLUSION: These results suggest that sensation seeking assessed with the SSS is not a temperament characteristic associated with bipolar disorders but is instead linked to a tendency towards alcohol abuse.
|
['Adult', 'Age Distribution', 'Bipolar Disorder', 'Comorbidity', 'Exploratory Behavior', 'Female', 'Humans', 'Male', 'Middle Aged', 'Psychiatric Status Rating Scales', 'Sex Distribution', 'Substance-Related Disorders', 'Temperament']
| 11,514,131
|
[['M01.060.116'], ['I01.240.050', 'N01.224.033', 'N06.850.505.400.050'], ['F03.084.500'], ['N05.715.350.225', 'N06.850.490.687'], ['F01.145.387', 'F01.658.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F04.711.513.653'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['C25.775', 'F03.900'], ['F01.752.898']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Risk of diabetes-associated diseases in subgroups of patients with recent-onset diabetes: a 5-year follow-up study.
|
BACKGROUND: Cluster analyses have proposed different diabetes phenotypes using age, BMI, glycaemia, homoeostasis model estimates, and islet autoantibodies. We tested whether comprehensive phenotyping validates and further characterises these clusters at diagnosis and whether relevant diabetes-related complications differ among these clusters, during 5-years of follow-up.METHODS: Patients with newly diagnosed type 1 or type 2 diabetes in the German Diabetes Study underwent comprehensive phenotyping and assessment of laboratory variables. Insulin sensitivity was assessed using hyperinsulinaemic-euglycaemic clamps, hepatocellular lipid content using magnetic resonance spectroscopy, hepatic fibrosis using non-invasive scores, and peripheral and autonomic neuropathy using functional and clinical criteria. Patients were reassessed after 5 years. The German Diabetes Study is registered with ClinicalTrials.gov, number NCT01055093, and is ongoing.FINDINGS: 1105 patients were classified at baseline into five clusters, with 386 (35%) assigned to mild age-related diabetes (MARD), 323 (29%) to mild obesity-related diabetes (MOD), 247 (22%) to severe autoimmune diabetes (SAID), 121 (11%) to severe insulin-resistant diabetes (SIRD), and 28 (3%) to severe insulin-deficient diabetes (SIDD). At 5-year follow-up, 367 patients were reassessed, 128 (35%) with MARD, 106 (29%) with MOD, 88 (24%) with SAID, 35 (10%) with SIRD, and ten (3%) with SIDD. Whole-body insulin sensitivity was lowest in patients with SIRD at baseline (mean 4·3 mg/kg per min [SD 2·0]) compared with those with SAID (8·4 mg/kg per min [3·2]; p<0·0001), MARD (7·5 mg/kg per min [2·5]; p<0·0001), MOD (6·6 mg/kg per min [2·6]; p=0·0011), and SIDD (5·5 mg/kg per min [2·4]; p=0·0035). The fasting adipose-tissue insulin resistance index at baseline was highest in patients with SIRD (median 15·6 [IQR 9·3-20·9]) and MOD (11·6 [7·4-17·9]) compared with those with MARD (6·0 [3·9-10·3]; both p<0·0001) and SAID (6·0 [3·0-9·5]; both p<0·0001). In patients with newly diagnosed diabetes, hepatocellular lipid content was highest at baseline in patients assigned to the SIRD cluster (median 19% [IQR 11-22]) compared with all other clusters (7% [2-15] for MOD, p=0·00052; 5% [2-11] for MARD, p<0·0001; 2% [0-13] for SIDD, p=0·0083; and 1% [0-3] for SAID, p<0·0001), even after adjustments for baseline medication. Accordingly, hepatic fibrosis at 5-year follow-up was more prevalent in patients with SIRD (n=7 [26%]) than in patients with SAID (n=5 [7%], p=0·0011), MARD (n=12 [12%], p=0·012), MOD (n=13 [15%], p=0·050), and SIDD (n=0 [0%], p value not available). Confirmed diabetic sensorimotor polyneuropathy was more prevalent at baseline in patients with SIDD (n=9 [36%]) compared with patients with SAID (n=10 [5%], p<0·0001), MARD (n=39 [15%], p=0·00066), MOD (n=26 [11%], p<0·0001), and SIRD (n=10 [17%], p<0·0001).INTERPRETATION: Cluster analysis can characterise cohorts with different degrees of whole-body and adipose-tissue insulin resistance. Specific diabetes clusters show different prevalence of diabetes complications at early stages of non-alcoholic fatty liver disease and diabetic neuropathy. These findings could help improve targeted prevention and treatment and enable precision medicine for diabetes and its comorbidities.FUNDING: German Diabetes Center, German Federal Ministry of Health, Ministry of Culture and Science of the state of North Rhine-Westphalia, German Federal Ministry of Education and Research, German Diabetes Association, German Center for Diabetes Research, Research Network SFB 1116 of the German Research Foundation, and Schmutzler Stiftung.
|
['Adult', 'Cluster Analysis', 'Diabetes Complications', 'Diabetes Mellitus, Type 1', 'Diabetes Mellitus, Type 2', 'Female', 'Follow-Up Studies', 'Gas Chromatography-Mass Spectrometry', 'Germany', 'Glucose Clamp Technique', 'Humans', 'Insulin Resistance', 'Male', 'Middle Aged', 'Phenotype', 'Time Factors']
| 31,345,776
|
[['M01.060.116'], ['E05.318.740.250', 'N05.715.360.750.200', 'N06.850.520.830.250'], ['C19.246.099'], ['C18.452.394.750.124', 'C19.246.267', 'C20.111.327'], ['C18.452.394.750.149', 'C19.246.300'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['E05.196.181.349.500', 'E05.196.566.500'], ['Z01.542.315'], ['E01.370.225.124.100.350', 'E05.196.500', 'E05.200.124.100.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.394.968.500', 'G07.690.773.984.617'], ['M01.060.116.630'], ['G05.695'], ['G01.910.857']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Geographicals [Z]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175.
|
Herpes simplex virus (HSV)-specific proteins fall into at least three kinetic classes whose synthesis is sequentially and coordinaely regulated. Temperature-sensitive (ts) mutants of one complementation group (1-2) are defective in the transition from immediate early to early and late protein synthesis. To elucidate the function of the 1-2 gene product in the HSV type 1 replicative cycle, nine ts mutants in this group were mapped by fine-structure analysis and characterized members of the group lie within the terminally repeated sequences of the S region of the genome. Fine-structure genetic and physical mapping permitted the mutations to be ordered within these sequences. Because it has been shown that the message for VP175 and the DNA template specifying this protein extend beyond the limits of the physical map of the mutations, it follows that the mutations must lie within the structural gene for VP175. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that most members of the group overproduced the immediate early proteins VP175, -136, -110, and -63 and markedly underproduced early and late proteins at the nonpermissive temperature. In temperature shiftup experiments, it was fund that the synthesis of early and late proteins ceased, whereas the synthesis of immediate early proteins began again. Thus, it is postulated that VP175 is (i) involved in the transition from immediate early to early protein synthesis, (ii) requird continuously to maintain early protein synthesis, (iii) autoregulated, acting to inhibit immediate early protein synthesis.
|
['Chromosome Mapping', 'Gene Expression Regulation', 'Genes', 'Genes, Viral', 'Genetic Markers', 'Mutation', 'Simplexvirus', 'Transcription, Genetic', 'Viral Proteins']
| 6,255,206
|
[['E05.393.183'], ['G05.308'], ['G05.360.340.024.340'], ['G05.360.340.024.340.364.875', 'G05.360.340.358.024.875', 'G05.360.340.358.840.500'], ['D23.101.387', 'G05.695.450'], ['G05.365.590'], ['B04.280.382.100.750'], ['G02.111.873', 'G05.297.700'], ['D12.776.964']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A custom speech valve retainer for the laryngectomee.
|
A technique is presented for the fabrication of a custom silicone speech valve retainer that is easily fabricated, may be tinted to match the skin tone, is well tolerated, noninvasive, and functions well in patients who can generate sufficient air pressure to close the valve. This device can greatly improve the quality of life for laryngectomees by allowing hands-free, alaryngeal oral speech.
|
['Colloids', 'Esthetics', 'Humans', 'Laryngectomy', 'Larynx, Artificial', 'Prosthesis Design', 'Silicone Elastomers', 'Silicones']
| 3,470,512
|
[['D20.280', 'D26.255.165'], ['F02.463.785.477', 'K01.752.210'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E04.580.369'], ['E07.695.450', 'E07.858.082.595'], ['E05.320.550', 'E07.695.680'], ['D05.750.900.850.900', 'D25.720.327.900', 'D25.720.900.850.900', 'J01.637.051.720.327.900', 'J01.637.051.720.900.850.900', 'J01.637.412.900'], ['D02.756.650.700', 'D05.750.900.850', 'D25.720.900.850', 'J01.637.051.720.900.850']]
|
['Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Humanities [K]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
A qualitative investigation of the attitudes and beliefs about physical activity and post-traumatic osteoarthritis in young adults 3-10 years after an intra-articular knee injury.
|
OBJECTIVE: To understand the influence of the injury experience on current attitudes and beliefs about physical activity (PA) and the development of post-traumatic osteoarthritis (PTOA) in youth and young adults 3-10 years after a sport-related knee injury.DESIGN: Qualitative study.SETTING: University Sports Medical/Research Center.PARTICIPANTS: 20 young adults 3-10 years subsequent to intra-articular knee injury.MAIN OUTCOME MEASURES: Semi-structured interviews were conducted and transcribed verbatim. Analysis used a constant comparative approach with conceptual labels and categories, axial coding, and selective coding to reveal main themes.RESULTS: The four main themes were: acceptance; resiliency and determination; knee confidence; and athletic identity. Participants accepted the impact of the injury on their sporting ability and future PTOA to varying degrees. Participants were often highly motivated to recover and met the injury with resilience. Knee confidence was a major concern. Most participants' athletic identity had evolved; impacted both by life and injury experiences.CONCLUSIONS: This study provides insight into the knee injury experience and resultant attitudes and beliefs regarding PA and PTOA in adolescents. Physiotherapists may assist in secondary prevention of PTOA by promoting PA, addressing knee confidence, and educating about long-term joint health.
|
['Adaptation, Psychological', 'Adolescent', 'Athletes', 'Athletic Injuries', 'Attitude', 'Exercise', 'Female', 'Humans', 'Knee Injuries', 'Male', 'Osteoarthritis, Knee', 'Self Concept', 'Young Adult']
| 29,778,829
|
[['F01.058'], ['M01.060.057'], ['M01.072'], ['C26.115'], ['F01.100'], ['G11.427.410.698.277', 'I03.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C26.558.554'], ['C05.550.114.606.500', 'C05.799.613.500'], ['F01.752.747.792'], ['M01.060.116.815']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
|
Neurobehavioral toxicity of long-term exposure to tetrachloroethylene.
|
One hundred and one employees of dry cleaning shops exposed to tetrachloroethylene (time weighted average 205 mg/m3) and 84 employees of departmental stores and hotels were compared from the results of a psychological examination. Age, gender, the daily consumption of alcohol and the intellectual level were taken into consideration analysing the effects of tetrachloroethylene. Perceptual speed, digit reproduction as a memory test, the digit symbol test as a substitution task and variables of a choice reaction test as well as a cancellation test differed significantly between the controls on one hand, and the groups of low and high exposure on the other. But, the differences between the exposure groups were not significant. There was no effect of alcohol on the exposure-related group differences. By means of discriminant analyses the diagnostic effectiveness of the biochemical, neurological and psychological methods were compared to classify the subjects into exposure groups. The highest rate of correct classifications was performed by the multidisciplinary combination of approaches.
|
['Alcohol Drinking', 'Behavior', 'Discriminant Analysis', 'Hazardous Substances', 'Humans', 'Intelligence', 'Memory', 'Perception', 'Personality', 'Psychomotor Performance', 'Tetrachloroethylene']
| 2,626,148
|
[['F01.145.317.269'], ['F01.145'], ['E05.318.740.350', 'N05.715.360.750.325', 'N06.850.520.830.350'], ['D27.888.426'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.752.543'], ['F02.463.425.540'], ['F02.463.593'], ['F01.752'], ['F02.808', 'G11.427.700', 'G11.561.660'], ['D02.455.526.439.880']]
|
['Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.