Title
stringlengths 1
395
⌀ | abstractText
stringlengths 57
5.98k
| meshMajor
stringlengths 14
1.03k
| pmid
int64 22
33.2M
| meshid
stringlengths 2
3.14k
| meshroot
stringlengths 2
421
| A
int64 0
1
| B
int64 0
1
| C
int64 0
1
| D
int64 0
1
| E
int64 0
1
| F
int64 0
1
| G
int64 0
1
| H
int64 0
1
| I
int64 0
1
| J
int64 0
1
| L
int64 0
1
| M
int64 0
1
| N
int64 0
1
| Z
int64 0
1
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
[Morphological changes after permanent nerve block by freezing and ethanol injection of the sciatic nerve of the rabbit (author's transl)].
|
Permanent nerve blocks by intraneurally injected alcohol are often complicated by alcohol-neuritis. Encouraging clinical experiences with permanent blocks by freezing raises the question whether morphological differences between the nerve lesions could explain the difference in their side effects. On 30 rabbits both sciatic nerves were blocked after surgical preparation. The one by intraneural injection of 0,5 ml of 96% Ethanol, the other by freezing with a cryoprobe. The resulting degeneration and the beginning of recovery of the nerves was followed by histological evaluations of the sciatic nerves during the first 28 days after the blockade. The nerve lesions of both types of blockade were complete. That produced by the cryoprobe was limited to the small area of local freezing, whereas the alcohol-block produced the same type of nerve degeneration but with a wide-spread extension reaching the sacral plexus. We discuss whether this slight morphological difference might be sufficient to explain the higher complication rate of alcohol blocks.
|
['Animals', 'Ethanol', 'Freezing', 'Nerve Block', 'Rabbits', 'Sciatic Nerve']
| 7,071,393
|
[['B01.050'], ['D02.033.375'], ['G01.645.500', 'G01.906.595.272.437', 'G02.734.466'], ['E03.155.086.711', 'E04.525.210.550'], ['B01.050.150.900.649.313.968.700'], ['A08.800.800.720.450.760']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Histological diagnosis of mediastinal lymph node metastases from renal cell carcinoma by endobronchial ultrasound-guided transbronchial needle aspiration.
|
Evaluation of mediastinal lymphadenopathy in patients with an intrathoracic nodule post malignancy is crucial for the determination of further treatment. Different radiological modalities are available for the detection of mediastinal lymph node metastases such as multidetector helical CT, PET-scan and PET-CT. However, tissue sampling is required for a firm diagnosis. A minimally invasive method of tissue sampling of mediastinal and hilar lymph nodes using direct real-time endobronchial ultrasound-guided transbronchial needle aspiration has been reported. This method is appropriate not only for cytodiagnosis but also for histological diagnosis. This current study reports a case of mediastinal lymph node metastases from renal cell carcinoma successfully diagnosed histologically by endobronchial ultrasound-guided transbronchial needle aspiration.
|
['Biopsy, Fine-Needle', 'Bronchoscopy', 'Carcinoma, Renal Cell', 'Diagnosis, Differential', 'Endosonography', 'Humans', 'Kidney Neoplasms', 'Lymph Nodes', 'Lymphatic Metastasis', 'Male', 'Mediastinum', 'Middle Aged']
| 17,298,469
|
[['E01.370.225.500.384.100.119.500', 'E01.370.225.998.054.119.500', 'E01.370.388.100.100.500', 'E04.074.119.500', 'E04.665.100.500', 'E05.200.500.384.100.119.500', 'E05.200.998.054.119.500', 'E05.242.384.100.119.500'], ['E01.370.386.105', 'E01.370.388.250.100', 'E04.502.250.100', 'E04.928.600.080'], ['C04.557.470.200.025.390', 'C04.588.945.947.535.160', 'C12.758.820.750.160', 'C12.777.419.473.160', 'C13.351.937.820.535.160', 'C13.351.968.419.473.160'], ['E01.171'], ['E01.370.350.850.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.945.947.535', 'C12.758.820.750', 'C12.777.419.473', 'C13.351.937.820.535', 'C13.351.968.419.473'], ['A10.549.400', 'A15.382.520.604.412'], ['C04.697.650.560', 'C23.550.727.650.560'], ['A01.923.761.800.500'], ['M01.060.116.630']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Named Groups [M]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Medical ethics and truth telling in the case of androgen insensitivity syndrome.
|
Should a physician always tell the truth to a patient? Is biomedical ethics too "politically correct" in certain situations? The second-place winner in the 1995 Logie Medical Ethics Essay Contest discusses whether telling the truth is the proper course for a physician dealing with certain patients.
|
['Androgen-Insensitivity Syndrome', 'Ethics, Medical', 'Female', 'Humans', 'Male', 'Truth Disclosure']
| 8,630,847
|
[['C12.706.316.096.500', 'C13.351.875.253.096.500', 'C16.131.939.316.096.500', 'C16.320.322.061', 'C19.391.119.096.500'], ['K01.752.566.479.171.132.750', 'N05.350.340.162.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.401.046.800', 'I01.880.604.583.080.134.800']]
|
['Diseases [C]', 'Humanities [K]', 'Health Care [N]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
|
Genetic characterization of the secretory mutant MS-1 of Tetrahymena thermophila: vacuolarization and block in secretion of lysosomal hydrolases are caused by a single gene mutation.
|
The genetics and phenotypic features (light and electron microscopy) of a secretory mutant, MS-1 of Tetrahymena thermophila blocked in secretion of lysosomal acid hydrolases have been analyzed. Although blocked constitutively in secretion, MS-1 contains active lysosomal hydrolases in amounts equivalent to the wild type. The 3:1 segregation in F-2 in sib crosses and the 1:1 segregation in test crosses indicate that the block in secretion of lysosomal hydrolases is controlled by a recessive single gene locus termed sec. The sec allele of MS-1 proved also to be responsible for the highly vacuolarized phenotype the mutant developed when it was transferred from nutrient medium into buffers of low ionic strength. Deletion mapping by crossing MS-1 with nullisomic strains, all secreting lysosomal hydrolases at wild-type rates, was performed. The sec phenotype was expressed in monosomic-4 progeny only, indicating that the sec allele is located on chromosome 4 of T. thermophila.
|
['Alleles', 'Animals', 'Chromosome Mapping', 'Crosses, Genetic', 'Hydrolases', 'Lysosomes', 'Mutation', 'Phenotype', 'Tetrahymena thermophila', 'Vacuoles']
| 1,499,158
|
[['G05.360.340.024.340.030'], ['B01.050'], ['E05.393.183'], ['E05.393.281'], ['D08.811.277'], ['A11.284.430.214.190.875.190.550'], ['G05.365.590'], ['G05.695'], ['B01.043.185.650.375.750.850.875'], ['A11.284.430.214.190.875.190.920']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Aging and ESRD demographics: consequences for the practice of dialysis.
|
The disproportionate increase in the prevalence of chronic kidney disease (CKD) and end-stage renal disease (ESRD) in the elderly is now recognized as a national and global reality. Among the major contributing factors are the aging of the population, a growing prevalence of CKD, greater access to care, and increased comorbidities. The utilization of renal replacement therapy in the geriatric population has concomitantly increased. It is imposing enormous challenges to the practice of ESRD care, the largest of which may be to determine the best application of clinical performance targets to a population with limitations in life expectancy. Concurrently, increased focus on quality of life will be required. The effective dialysis practitioner will need to adapt to the aging ESRD demographics with an increased focus on physical and mental well-being of the geriatric patient.
|
['Age Factors', 'Aged', 'Humans', 'Kidney Failure, Chronic', 'Quality of Life', 'Renal Dialysis']
| 23,067,122
|
[['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.777.419.780.750.500', 'C13.351.968.419.780.750.500'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E02.870.300', 'E02.912.800']]
|
['Health Care [N]', 'Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Humanities [K]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Entomological indices of malaria transmission in Chikhwawa district, Southern Malawi.
|
BACKGROUND: Although malaria is highly prevalent throughout Malawi, little is known of its transmission dynamics. This paper describes the seasonal activity of the different vectors, human biting indices, sporozoite rates and the entomological inoculation rate in a low-lying rural area in southern Malawi.METHODS: Vectors were sampled over 52 weeks from January 2002 to January 2003, by pyrethrum knockdown catch in two villages in Chikhwawa district, in the Lower Shire Valley.RESULTS: In total, 7,717 anophelines were collected of which 55.1% were Anopheles gambiae sensu lato and 44.9% were Anopheles funestus. Three members of the An. gambiae complex were identified by PCR: Anopheles arabiensis (75%) was abundant throughout the year, An. gambiae s.s. (25%) was most common during the wet season and Anopheles quadriannulatus occurred at a very low frequency (n=16). An. funestus was found in all samples but was most common during the dry season.Anopheles gambiae s.s. and An. funestus were highly anthropophilic with human blood indices of 99.2% and 96.3%, respectively. Anopheles arabiensis had fed predominantly on humans (85.0%) and less commonly on cattle (10.9%; 1.2% of blood meals were of mixed origin). Plasmodium falciparum (192/3,984) and Plasmodium malariae (1/3,984) sporozoites were detected by PCR in An. arabiensis (3.2%) and An. funestus (4.5%), and in a significantly higher proportion of An. gambiae s.s. (10.6%)(p<0.01). All three vectors were present throughout the year and malaria transmission occurred in every month, although with greatest intensity during the rainy season (January to April). The combined human blood index exceeded 92% and the P. falciparum sporozoite rate was 4.8%, resulting in estimated inoculation rates of 183 infective bites/ person per annum, or an average rate of ~15 infective bites/person/month.CONCLUSIONS: The results demonstrate the importance of An. gambiae s.s., An. arabiensis and An. funestus in driving the high levels of malaria transmission in the south of Malawi. Sustained and high coverage or roll out of current approaches to malaria control (primarily insecticide-treated bed nets and indoor residual house spraying) in the area are likely to reduce the observed high malaria transmission rate and consequently the incidence of human infections, unless impeded by increasing resistance of vectors to insecticides.
|
['Animals', 'Anopheles', 'Cattle', 'Female', 'Humans', 'Insect Vectors', 'Malaria', 'Malawi', 'Mosquito Control', 'Oocysts', 'Plasmodium', 'Rural Population', 'Seasons', 'Sporozoites']
| 23,171,123
|
[['B01.050'], ['B01.050.500.131.617.720.500.500.750.712.500.875.120'], ['B01.050.150.900.649.313.500.380.271'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N06.850.335.188.100.500', 'N06.850.520.203.375.100.500'], ['C01.610.752.530', 'C01.920.875'], ['Z01.058.290.175.500'], ['N06.850.780.200.650.425.500'], ['A11.870.740.600', 'B05.500.675', 'B05.775.740.600', 'G07.345.500.550.500.675'], ['B01.043.075.380.611'], ['N01.600.725'], ['G01.910.645.661', 'G16.500.275.071.590', 'N06.230.300.100.250.525'], ['A11.870.740.600.800', 'B05.500.675.800', 'B05.775.740.600.800', 'G07.345.500.550.500.675.800']]
|
['Organisms [B]', 'Health Care [N]', 'Diseases [C]', 'Geographicals [Z]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Enhanced cytotoxic activity of macrophages and suppressed tumor metastases in mice irradiated with low doses of X- rays.
|
We showed in our previous report that a single exposure of mice to 0.1 or 0.2 Gy X-rays led to the significant inhibition of the development of artificial tumor metastases in the lungs and that the effect was related to the enhanced activity of natural killer cells. In the present study, a possible involvement of cytotoxic macrophages in the anti-metastatic effect of the low-level X-ray exposures was investigated. We now demonstrate that irradiation of mice with either of the two low doses of X-rays significantly stimulates the macrophage-mediated cytolysis of the susceptible tumor targets and that the effect coincides with the enhanced production of nitric oxide in the collected effector cells. We also show that suppression of the in vivo function of macrophages by carrageenan eliminates the inhibitory effect of the two low doses of X-rays on the development of pulmonary tumor colonies as well as significantly suppresses the macrophage-mediated cytotoxicity and nitric oxide production. Finally, aminoguanidine added to the culture medium of the assayed macrophages not only shuts down the nitric oxide synthesis in these cells but also significantly suppresses their cytolytic activity. Overall, the obtained results indicate that inhibition of the tumor metastases by a single exposure of mice to 0.1 or 0.2 Gy X-rays results, to a large extent, from the radiation-induced stimulation of the cytocidal activity of macrophages which secrete enhanced amounts of nitric oxide.
|
['Animals', 'Cell Line, Tumor', 'Cell Proliferation', 'Cell Survival', 'Dose-Response Relationship, Radiation', 'Lung Neoplasms', 'Macrophage Activation', 'Macrophages', 'Male', 'Mice', 'Mice, Inbred BALB C', 'Radiation Dosage', 'X-Rays']
| 16,936,416
|
[['B01.050'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.161.750', 'G07.345.249.410.750'], ['G04.346'], ['E05.799.513.500', 'G01.750.740.500', 'G04.712.500', 'G07.225', 'G07.738.500', 'N06.850.810.250.180'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['G12.287.500'], ['A11.329.372', 'A11.627.482', 'A11.733.397', 'A15.382.670.522', 'A15.382.680.397'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.338', 'B01.050.150.900.649.313.992.635.505.500.400.338'], ['E05.799.513', 'G01.750.740', 'N06.850.810.250'], ['G01.358.500.505.970', 'G01.750.250.970', 'G01.750.750.918']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Investigating linear and interactive effects of shared mental models on safety and efficiency in a field setting.
|
Linkages between 2 types of shared mental models (SMMs)--that is, positional-goal interdependencies and cue-strategy associations--and effectiveness in an air traffic control environment were investigated. Two types of SMMs were expected to contribute uniquely, as well as interact, to predict tower safety and efficiency. Using SMM data from 306 air traffic controllers, and corresponding archival efficiency and safety measures for 47 airports, the authors found no significant linear relationships between SMMs and either outcome measure. However, the 2 SMMs interacted with one another to predict both outcomes. Results are discussed in terms of the importance of measuring multiple types of SMMs, the examination of complex relationships, and the importance of indexing decisions.
|
['Adult', 'Aircraft', 'Cooperative Behavior', 'Cues', 'Efficiency', 'Female', 'Humans', 'Linear Models', 'Male', 'Models, Psychological', 'Organizational Objectives', 'Personal Construct Theory', 'Problem Solving', 'Safety']
| 15,910,147
|
[['M01.060.116'], ['J01.937.285.100'], ['F01.145.813.115'], ['F02.463.425.234'], ['F02.784.692.351', 'N04.452.209'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.500.500', 'E05.318.740.750.425', 'E05.599.835.750', 'N05.715.360.750.530.460', 'N05.715.360.750.695.460', 'N06.850.520.830.500.500', 'N06.850.520.830.750.425'], ['E05.599.695'], ['N04.452.615'], ['F02.739.660'], ['F02.463.425.725', 'F02.463.785.810'], ['N06.850.135.060.075']]
|
['Named Groups [M]', 'Technology, Industry, and Agriculture [J]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 1
| 0
|
[Picotamide does not interfere with the anticoagulant activity of warfarin in patients wearing heart valve prostheses].
|
Picotamide, a new antiplatelet drug which has only slight effects on bleeding time, could be useful, in combination with oral anticoagulants, for the prevention of thromboembolic complications in patients with heart valve prostheses. We have evaluated in a randomized, controlled, double-blind, cross-over study, the effect of picotamide on the anticoagulant activity of warfarin. Administration of 300 mg t.i.d. for 10 days to 10 patients with aortic or mitral valve prostheses did not modify significantly either the level of anticoagulation or the mean daily dosage of warfarin. We observed a trend towards a reduction of plasma levels of beta-thromboglobulin. In conclusion the results of this study show that picotamide does not interfere with the anticoagulant activity of warfarin.
|
['Aged', 'Aortic Valve', 'Double-Blind Method', 'Drug Interactions', 'Drug Therapy, Combination', 'Female', 'Heart Valve Prosthesis', 'Humans', 'Male', 'Middle Aged', 'Mitral Valve', 'Phthalic Acids', 'Platelet Aggregation Inhibitors', 'Thromboembolism', 'Warfarin']
| 2,151,435
|
[['M01.060.116.100'], ['A07.541.510.110'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['G07.690.773.968'], ['E02.319.310'], ['E07.695.310'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A07.541.510.507'], ['D02.241.223.805'], ['D27.505.954.502.780'], ['C14.907.355.590'], ['D03.383.663.283.446.520.914', 'D03.633.100.150.446.520.914']]
|
['Named Groups [M]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Caregiving, Transport-Related, and Demographic Correlates of Sedentary Behavior in Older Adults: The Senior Neighborhood Quality of Life Study.
|
OBJECTIVE: Excess sedentary time predicts negative health outcomes independent of physical activity. The present investigation examined informal caregiving duties and transportation-related factors as potential correlates of sedentary behavior in older adults.METHOD: Average daily sedentary time was measured via accelerometer in adults ages 66 years and older (N = 861). Caregiving variables included dog ownership and informal family caregiving status. Transportation variables included driver status, walking distance to public transit, and reported presence of pedestrians and bicyclists in one's neighborhood.RESULTS: In multivariate models, owning a dog and being a driver were associated with less sedentary time (p ? .01). Educational status and geographic region modified the association between dog ownership and sedentary time, and age modified the association between driver status and sedentary time.DISCUSSION: This study identified that older adult dog owners and drivers were less sedentary. Both factors may create opportunities for older adults to get out of their homes.
|
['Age Factors', 'Aged', 'Animals', 'Automobile Driving', 'Caregivers', 'Dogs', 'Educational Status', 'Female', 'Geography', 'Humans', 'Male', 'Multivariate Analysis', 'Ownership', 'Quality of Life', 'Residence Characteristics', 'Sedentary Behavior', 'Transportation', 'Walking']
| 26,538,268
|
[['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['B01.050'], ['I03.125'], ['M01.085', 'M01.526.485.200', 'N02.360.200'], ['B01.050.150.900.649.313.750.250.216.200'], ['N01.824.196'], ['H01.277.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.740.150.500', 'N05.715.360.750.125.500', 'N06.850.520.830.150.500'], ['I01.880.604.583.594', 'N04.452.633'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['N01.224.791', 'N06.850.505.400.800'], ['F01.145.749', 'F01.829.458.705'], ['J01.937'], ['G11.427.410.568.900', 'G11.427.410.698.277.937', 'I03.350.937', 'I03.450.642.845.940']]
|
['Health Care [N]', 'Named Groups [M]', 'Organisms [B]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Humanities [K]', 'Psychiatry and Psychology [F]', 'Technology, Industry, and Agriculture [J]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
|
Conversion of AFLP bands into high-throughput DNA markers.
|
The conversion of AFLP bands into polymorphic sequence-tagged-site (STS) markers is necessary for high-throughput genotype scoring. Technical hurdles that must be overcome arise from genome complexity (particularly sequence duplication), from the low-molecular-weight nature of the AFLP bands and from the location of the polymorphism within the AFLP band. We generated six STS markers from ten AFLP bands (four AFLPs were from co-dominant pairs of bands) in soybean (Glycine max). The markers were all linked to one of two loci, rhg1 on linkage group G and Rhg4 on linkage group A2, that confer resistance to the soybean cyst nematode (Heterodera glycines I.). When the polymorphic AFLP band sequence contained a duplicated sequence or could not be converted to a locus-specific STS marker, direct sequencing of BAC clones anchored to a physical map generated locus-specific flanking sequences at the polymorphic locus. When the polymorphism was adjacent to the restriction site used in the AFLP analysis, single primer extension was performed to reconstruct the polymorphism. The six converted AFLP markers represented 996 bp of sequence from alleles of each of two cultivars and identified eight insertions or deletions, two microsatellites and eight single-nucleotide polymorphisms (SNPs). The polymorphic sequences were used to design a non-electrophoretic, fluorometric assay (based on the TaqMan technology) and/or develop electrophoretic STS markers for high-throughput genotype determination during marker-assisted breeding for resistance to cyst nematode. We conclude that the converted AFLP markers contained polymorphism at a 10- to 20-fold higher frequency than expected for adapted soybean cultivars and that the efficiency of AFLP band conversion to STS can be improved using BAC libraries and physical maps. The method provides an efficient tool for SNP and STS discovery suitable for marker-assisted breeding and genomics.
|
['Alleles', 'Base Sequence', 'Cloning, Molecular', 'DNA, Plant', 'Genetic Markers', 'Molecular Sequence Data', 'Polymerase Chain Reaction', 'Polymorphism, Genetic', 'Sequence Tagged Sites', 'Soybeans']
| 11,361,330
|
[['G05.360.340.024.340.030'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.220'], ['D13.444.308.435'], ['D23.101.387', 'G05.695.450'], ['L01.453.245.667'], ['E05.393.620.500'], ['G05.365.795'], ['G05.360.340.024.810'], ['B01.650.940.800.575.912.250.401.750']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Rapid diagnosis of oral herpes simplex or zoster virus infections by immunofluorescence: comparison with Tzanck cell preparations and viral culture.
|
This study compared viral culture with routine cytology and immunofluorescent staining of oral epithelial cell smears for the diagnosis of orofacial infections due to herpes simplex virus (HSV) or herpes zoster (HZ). Twenty-one patients were studied. Viral culture gave the greatest number of positive results, with an assumed sensitivity and specificity of 100%, but the test took at least 24 hours. For haematoxylin and eosin cytology, the corresponding figures for sensitivity and specificity were 54% and 100%, respectively. Direct staining of epithelial smears with FITC-conjugated monoclonal antibodies to HSV type 1, HSV type 2 and HZ had a sensitivity of 82% and specificity of 71%. Smears prepared from oral washings, and stained with the same fluorescent antibodies, yielded a sensitivity of 83% and specificity of 86%. To allow rapid diagnosis, oral epithelial smears for immunofluorescent examination should also be prepared, since in more than 80% of cases such smears will confirm an herpetic infection in less than one hour.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Antibodies, Monoclonal', 'Child', 'Child, Preschool', 'Fluorescent Antibody Technique', 'Herpes Zoster', 'Histological Techniques', 'Humans', 'Middle Aged', 'Mouth Diseases', 'Sensitivity and Specificity', 'Stomatitis, Herpetic']
| 2,675,949
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['M01.060.406'], ['M01.060.406.448'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['C01.925.256.466.930.750'], ['E01.370.225.750', 'E05.200.750'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C07.465'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['C01.925.256.466.382.834', 'C07.465.864.937']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
7-Substituted 2-phenyl-benzofurans as ER beta selective ligands.
|
A series of 2-(4-hydroxy-phenyl)-benzofuran-5-ols with relatively lipophilic groups in the 7-position of the benzofuran was prepared and the affinity and selectivity for ER beta was measured. Many of the analogues were found to be potent and selective ER beta ligands. Additional modifications at the benzofuran 4-position as well as at the 3'-position of the 2-phenyl group were found to further increase selectivity. Such modifications led to compounds with <10 nM potency and >100-fold selectivity for ER beta.
|
['Benzofurans', 'Cell Line, Tumor', 'Estrogen Receptor beta', 'Humans', 'Insulin-Like Growth Factor Binding Protein 4', 'Ligands', 'RNA, Messenger', 'Structure-Activity Relationship']
| 15,341,953
|
[['D03.633.100.127'], ['A11.251.210.190', 'A11.251.860.180'], ['D12.776.826.750.350.262'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.157.420.280'], ['D27.720.470.480'], ['D13.444.735.544'], ['G02.111.830', 'G07.690.773.997']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Recurrent childhood anaplastic large cell lymphoma: a retrospective analysis of registered cases in Japan.
|
This report presents a retrospective study of 26 Japanese children with recurrent anaplastic large cell lymphoma. The first relapses were documented at a median of 10.5 months after the initial diagnosis. Twenty-four patients achieved a second remission. After a median follow-up period of 47 months, 18 patients are still alive: 15 patients are in second complete remission (CR), three patients are in third CR or later. The 5 year overall and relapse-free survival rates were 61 +/- 12% and 51 +/- 12% respectively. The patients who received allogeneic haematopoietic stem cell transplantation during second CR showed a superior outcome to other patients.
|
['Adolescent', 'Antineoplastic Combined Chemotherapy Protocols', 'Child', 'Child, Preschool', 'Disease Progression', 'Female', 'Follow-Up Studies', 'Hematopoietic Stem Cell Transplantation', 'Humans', 'Infant', 'Japan', 'Lymphoma, Large-Cell, Anaplastic', 'Male', 'Neoplasm Recurrence, Local', 'Remission Induction', 'Retrospective Studies', 'Survival Rate', 'Transplantation, Homologous', 'Treatment Outcome']
| 16,445,832
|
[['M01.060.057'], ['E02.183.750.500', 'E02.319.077.500', 'E02.319.310.037'], ['M01.060.406'], ['M01.060.406.448'], ['C23.550.291.656'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['E02.095.147.500.500.500', 'E04.936.225.687.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['Z01.252.474.463', 'Z01.639.595'], ['C04.557.386.480.750.399', 'C15.604.515.569.480.750.600', 'C20.683.515.761.480.750.399'], ['C04.697.655', 'C23.550.727.655'], ['E02.860'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['E04.936.864'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Subclavian steal syndrome from the ipsilateral vertebral artery.
|
We present two cases of subclavian steal syndrome from the ipsilateral vertebral artery (VA) to the subclavian artery (SCA), one with the left VA originating from the aortic arch and the other with the left VA originating very close to the origin of the SCA, proximal to the occluded segment. In both cases, angiographic demonstration of these anatomic variations and successful endovascular treatment are presented.
|
['Aged', 'Humans', 'Male', 'Middle Aged', 'Radiography', 'Subclavian Artery', 'Subclavian Steal Syndrome', 'Vertebral Artery']
| 15,205,155
|
[['M01.060.116.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E01.370.350.700'], ['A07.015.114.839'], ['C10.228.140.300.150.956.700', 'C14.907.253.092.956.700'], ['A07.015.114.955']]
|
['Named Groups [M]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Forced detachment of the CD2-CD58 complex.
|
The force-induced detachment of the adhesion protein complex CD2-CD58 was studied by steered molecular dynamics simulations. The forced detachment of CD2 and CD58 shows that the system can respond to an external force by two mechanisms, which depend on the loading rate. At the rapid loading rates of 70 and 35 pN/ps (pulling speeds of 1 and 0.5 A/ps) the two proteins unfold before they separate, whereas at slower loading rates of 7 and 3.5 pN/ps (pulling speeds of 0.1 and 0.05 A/ps), the proteins separate before the domains can unfold. When subjected to a constant force of 400 pN, the two proteins separated without significant structural distortion. These findings suggest that protein unfolding is not coupled to the adhesive function of CD2 and CD58. The simulations further confirm that salt bridges primarily determine the tensile strength of the protein-to-protein bond, and that the order of salt bridge rupture depends mainly on the position of the bond, relative to the line of action of the applied force. Salt bridges close to this line break first. The importance of each of the salt bridges for adhesion, determined from the simulations, correlates closely with their role in cell-to-cell adhesion and equilibrium binding determined by site-directed mutagenesis experiments.
|
['Binding Sites', 'CD2 Antigens', 'CD58 Antigens', 'Cell Adhesion', 'Computer Simulation', 'Crystallography', 'Macromolecular Substances', 'Models, Molecular', 'Motion', 'Protein Binding', 'Protein Conformation', 'Protein Denaturation', 'Protein Folding', 'Protein Structure, Tertiary', 'Static Electricity', 'Stress, Mechanical', 'Structure-Activity Relationship', 'Tensile Strength']
| 12,668,431
|
[['G02.111.570.120'], ['D23.050.301.264.894.090', 'D23.101.100.894.090'], ['D12.776.395.550.034', 'D12.776.543.550.158'], ['G04.022'], ['L01.224.160'], ['E05.196.309', 'H01.181.529.240'], ['D05'], ['E05.599.595'], ['G01.482'], ['G02.111.679', 'G03.808'], ['G02.111.570.820.709'], ['G01.154.651.750.500', 'G02.111.688.750.500'], ['G01.154.651', 'G02.111.688'], ['G02.111.570.820.709.610'], ['G01.358.500.249.820'], ['G01.374.835'], ['G02.111.830', 'G07.690.773.997'], ['G01.374.850']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Detection of prefracture hip lesions in atypical subtrochanteric fracture with dual-energy x-ray absorptiometry images.
|
PURPOSE: To retrospectively assess how often and how early hip dual-energy x-ray absorptiometry (DXA) images show prefracture lesions in patients with atypical subtrochanteric fracture (ASF) and determine whether DXA images with assessment of prodromal symptoms could be used for early ASF prediction.MATERIALS AND METHODS: The retrospective research protocol complied with HIPAA and was institutional review board approved, with waiver of informed consent. Among 62 women with ASF, nine without hip DXA images and seven without clear documentation of prodromal symptoms were excluded. Serial DXA images of 52 hips in 46 patients were included. Among them, 33 hips were assessed with ipsilateral DXA. For this ipsilateral group, each hip was assessed for prodromal symptoms and focal cortical changes in the lateral subtrochanteric femur cortex at DXA. Overall and cumulative detection rates for prodromal symptoms, DXA, and DXA with prodromal symptoms were measured and compared with a general linear model for overall detection rate and Cox proportional hazard models for cumulative detection rate. Thirty-three representative ipsilateral images and 199 images from subjects without fractures were reviewed in random order for prefracture lesions by three musculoskeletal radiologists independently, and the performance of DXA in ASF prediction was assessed.RESULTS: Overall detection rates for DXA, prodromal symptoms, and DXA with prodromal symptoms were 61% (20 of 33), 42% (14 of 33), and 73% (24 of 33), respectively, in the ipsilateral group. Overall detection rate comparisons showed that DXA with prodromal symptoms was superior to prodromal symptoms alone (P = .0377). The cumulative detection rate curve for DXA with prodromal symptoms was also superior to that of prodromal symptoms alone (P = .0018). Sensitivity and specificity of DXA in ASF prediction ranged from 52% (17 of 33) to 58% (19 of 33) and 99% (197 of 199) to 100% (199 of 199), respectively.CONCLUSION: Assessment of hip DXA images combined with conventional assessment of prodromal symptoms enables detection of more ASFs earlier than assessment based on prodromal symptoms alone.
|
['Absorptiometry, Photon', 'Aged', 'Aged, 80 and over', 'Early Diagnosis', 'Female', 'Hip Fractures', 'Humans', 'Middle Aged', 'Predictive Value of Tests', 'Retrospective Studies', 'Risk Factors']
| 24,126,368
|
[['E01.370.350.700.024', 'E05.196.712.224.187'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['E01.390'], ['C26.404.061.425', 'C26.531.750', 'C26.558.276.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Risk factors for ciliochoroidal effusion after panretinal photocoagulation.
|
PURPOSE: To determine the incidence, duration, and risk factors for ciliochoroidal effusion after panretinal photocoagulation.METHODS: Thirty-nine consecutive patients with diabetic retinopathy underwent ultrasound biomicroscopy of both eyes to image the ciliochoroidal space immediately before and 1 day after unilateral argon-green panretinal photocoagulation. Imaging was repeated on days 3, 7, and 14 in patients in whom ciliochoroidal effusion developed.RESULTS: Low-lying ciliochoroidal effusions were imaged in 23 (59%) of 39 eyes. Of 23 eyes, effusions resolved in 6 (26%), 12 (52%), and 5 (22%) eyes by 3, 7, and 14 days respectively. The number of laser applications (P = 0.02), shorter axial length (P = 0.01), and percentage of retinal surface area treated (P = 0.02) were associated with systemic hypertension, location of treatment, previous panretinal photocoagulation of cataract surgery, retinal surface area treated, and mean blood pressure before photocoagulation were not associated with effusion. All fellow, untreated eyes remained effusion-free.CONCLUSION: Ciliochoroidal effusion develops commonly after panretinal photocoagulation. Limiting the number of laser applications and the percentage of retinal surface area treated reduces the likelihood of this complication. Eyes with shorter axial lengths are at higher risk
|
['Adult', 'Aged', 'Anterior Eye Segment', 'Choroid Diseases', 'Ciliary Body', 'Diabetic Retinopathy', 'Female', 'Humans', 'Incidence', 'Laser Coagulation', 'Male', 'Middle Aged', 'Postoperative Complications', 'Prospective Studies', 'Retina', 'Risk Factors', 'Ultrasonography']
| 8,637,695
|
[['M01.060.116'], ['M01.060.116.100'], ['A09.371.060'], ['C11.941.160'], ['A09.371.060.160', 'A09.371.894.280'], ['C11.768.257', 'C14.907.320.382', 'C19.246.099.500.382'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E02.520.745.410', 'E02.594.530', 'E04.014.520.530', 'E04.350.750.410', 'E04.540.630.410'], ['M01.060.116.630'], ['C23.550.767'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['A09.371.729'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.370.350.850']]
|
['Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Internuclear ophthalmoplegia associated with anti-TNFá medication.
|
AIM: To describe the presentation of an internuclear ophthalmoplegia (INO) associated with the use of anti-tumor necrosis factor á (anti-TNFá) medication.METHODS: A case report of a woman, aged 27 years, who developed facial numbness, blurred vision, and diplopia on right gaze. She had a history of Crohn's disease, which was being treated by the anti-TNFá drug, adalimumab. On examination, a left INO was found.TREATMENT: The patient was prescribed a short course of corticosteroids and adalimumab treatment was discontinued.RESULTS: Magnetic resonance imaging demonstrated typical demyelinating lesions including one responsible for the INO. Following a short course of corticosteroids and the discontinuation of the adalimumab treatment, the INO resolved, resulting in a swift improvement of ocular motility over a 2-week period.CONCLUSION: Anti-TNFá therapies have been associated with the development of demyelinating diseases. The presentation of a brainstem syndrome in a patient on anti-TNFá therapy should lead to investigation for central nervous system demyelination and cessation of the medication.
|
['Adalimumab', 'Adult', 'Anti-Inflammatory Agents', 'Crohn Disease', 'Female', 'Glucocorticoids', 'Humans', 'Magnetic Resonance Imaging', 'Methylprednisolone', 'Ocular Motility Disorders', 'Tumor Necrosis Factor-alpha']
| 25,790,247
|
[['D12.776.124.486.485.114.224.060.250', 'D12.776.124.790.651.114.224.060.250', 'D12.776.377.715.548.114.224.200.250'], ['M01.060.116'], ['D27.505.954.158'], ['C06.405.205.731.500', 'C06.405.469.432.500'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['D04.210.500.745.432.769.795.539'], ['C10.228.758', 'C10.292.562', 'C11.590'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[102 patients with suspected myocarditis : Clinical presentation, diagnostics, therapy and prognosis].
|
INTRODUCTION: Myocarditis is a disease which is difficult to diagnose and which includes a risk of the development of dilated cardiomyopathy and sudden cardiac death.METHODS AND PATIENTS: In this study 102 patients were included from the time period 2003-2013 after diagnosis or suspected diagnosis of myocarditis in the department of internal medicine at the University Hospital Halle (Saale).RESULTS: Of the study participants 77.5% were male and the average age was 35.5 ± 14.1 years. The symptoms reported by the patients were angina in 46.1%, dyspnea in 38.2%, performance deterioration in 29.4%, palpitations in 9.8% and syncope in 8.8%. In 45.1% of patients, symptoms were preceded by a respiratory infection. All patients underwent an echocardiogram and in 36.5% it was possible to demonstrate a regional wall motion abnormality and in 20.4% a pericardial effusion. A myocardial biopsy was performed in 15.6% of the patients. The presence of cardiotropic viruses was investigated in 37.3% of patients but was detected in only 5.9%. Cardiac magnetic resonance imaging (MRI) was performed in 82 patients of whom 33.3% showed a late enhancement and 11.9% a wall movement disorder. In this study four patients, all male, died and three suffered recurrent myocarditis.CONCLUSION: This study showed the wide range of symptoms in myocarditis. Myocarditis is rarely severely manifested and in this study the mortality was 3.9%. For further optimization of the diagnostic and treatment algorithms, prospective, randomized studies would be desirable.
|
['Adult', 'Algorithms', 'Biomarkers', 'Biopsy', 'Cardiomyopathy, Dilated', 'Death, Sudden, Cardiac', 'Diagnosis, Differential', 'Female', 'Humans', 'Magnetic Resonance Imaging', 'Male', 'Middle Aged', 'Myocarditis', 'Myocardium', 'Prognosis', 'Recurrence', 'Retrospective Studies', 'Risk Factors', 'Young Adult']
| 28,101,623
|
[['M01.060.116'], ['G17.035', 'L01.224.050'], ['D23.101'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['C14.280.195.160', 'C14.280.238.070', 'C16.320.488.750'], ['C14.280.383.220', 'C23.550.260.322.250'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.825.500'], ['M01.060.116.630'], ['C14.280.238.625'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['E01.789'], ['C23.550.291.937'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Analysis of endocrine activity in drinking water, surface water and treated wastewater from six countries.
|
The aquatic environment can contain numerous micropollutants and there are concerns about endocrine activity in environmental waters and the potential impacts on human and ecosystem health. In this study a complementary chemical analysis and in vitro bioassay approach was applied to evaluate endocrine activity in treated wastewater, surface water and drinking water samples from six countries (Germany, Australia, France, South Africa, the Netherlands and Spain). The bioassay test battery included assays indicative of seven endocrine pathways, while 58 different chemicals, including pesticides, pharmaceuticals and industrial compounds, were analysed by targeted chemical analysis. Endocrine activity was below the limit of quantification for most water samples, with only two of six treated wastewater samples and two of six surface water samples exhibiting estrogenic, glucocorticoid, progestagenic and/or anti-mineralocorticoid activity above the limit of quantification. Based on available effect-based trigger values (EBT) for estrogenic and glucocorticoid activity, some of the wastewater and surface water samples were found to exceed the EBT, suggesting these environmental waters may pose a potential risk to ecosystem health. In contrast, the lack of bioassay activity and low detected chemical concentrations in the drinking water samples do not suggest a risk to human endocrine health, with all samples below the relevant EBTs.
|
['Biological Assay', 'Drinking Water', 'Ecosystem', 'Endocrine Disruptors', 'Environmental Monitoring', 'Glucocorticoids', 'Pesticides', 'Pharmaceutical Preparations', 'Receptors, Steroid', 'Receptors, Thyroid Hormone', 'Retinoid X Receptors', 'Waste Water', 'Water Pollutants, Chemical']
| 29,621,713
|
[['E05.091'], ['D01.045.250.875.300', 'D01.248.497.158.459.650.300', 'D01.650.550.925.199', 'G07.203.100.418', 'J02.200.418'], ['G16.500.275.157', 'N06.230.124'], ['D27.505.696.353', 'D27.888.141'], ['N06.850.460.350.080', 'N06.850.780.375'], ['D06.472.040.543', 'D27.505.696.399.472.488'], ['D27.720.031.700', 'D27.888.723'], ['D26'], ['D12.776.826.750', 'D12.776.930.778'], ['D12.776.624.664.700.830', 'D12.776.826.850'], ['D12.776.826.701.500', 'D12.776.930.775.500'], ['D20.944.932', 'N06.850.460.710.865'], ['D27.888.284.903.655']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Optimism for the Future in Younger and Older Adults.
|
OBJECTIVES: Research has suggested that older adults are less optimistic about their future than younger adults; however, a limitation of prior studies is that younger and older adults were forecasting to different ages and stages of life. To address this, we investigated whether there are age differences in future optimism when people project to the exact same age. We also tested whether optimism differs when projecting one's own future versus another person's future.METHOD: Participants were 285 younger and 292 older adults recruited from Amazon Mechanical Turk. Participants completed writing and word-rating tasks in which they imagined their own future in 15 years, their own future at age 85, or the average person's future at age 85.RESULTS: Younger adults were more optimistic than older adults about their own future in 15 years. In contrast, both age groups were similarly optimistic about their future at age 85 and expected it to be more positive than others' future at age 85.DISCUSSION: Contrary to previous research, younger and older adults had comparable future forecasts when projecting to the exact same age. These findings emphasize the need to consider age and stage of life when examining age differences in future optimism.
|
['Adolescent', 'Age Factors', 'Aged, 80 and over', 'Aging', 'Female', 'Forecasting', 'Human Development', 'Humans', 'Imagination', 'Male', 'Optimism', 'Psychology, Social']
| 29,325,140
|
[['M01.060.057'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100.080'], ['G07.345.124'], ['I01.320'], ['F01.525', 'G07.345.374'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463.188.634'], ['F01.100.787'], ['F01.829', 'F04.096.628.829']]
|
['Named Groups [M]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Psychiatry and Psychology [F]', 'Organisms [B]']
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Regulation of Anisotropic Tissue Growth by Two Orthogonal Signaling Centers.
|
The Drosophila wing has served as a paradigm to mechanistically characterize the role of morphogens in patterning and growth. Wingless (Wg) and Decapentaplegic (Dpp) are expressed in two orthogonal signaling centers, and their gradients organize patterning by regulating the expression of well-defined target genes. By contrast, graded activity of these morphogens is not an absolute requirement for wing growth. Despite their permissive role in regulating growth, here we show that Wg and Dpp are utilized in a non-interchangeable manner by the two existing orthogonal signaling centers to promote preferential growth along the two different axes of the developing wing. Our data indicate that these morphogens promote anisotropic growth by making use of distinct and non-interchangeable molecular mechanisms. Whereas Dpp drives growth along the anterior-posterior axis by maintaining Brinker levels below a growth-repressing threshold, Wg exerts its action along the proximal-distal axis through a double repression mechanism involving T cell factor (TCF).
|
['Animals', 'Drosophila Proteins', 'Drosophila melanogaster', 'Morphogenesis', 'Repressor Proteins', 'Signal Transduction', 'TCF Transcription Factors', 'Wings, Animal', 'Wnt1 Protein']
| 32,084,357
|
[['B01.050'], ['D12.776.093.500.462'], ['B01.050.500.131.617.720.500.500.750.310.250.500'], ['G07.345.500'], ['D12.776.260.703', 'D12.776.930.780'], ['G02.111.820', 'G04.835'], ['D12.776.260.730', 'D12.776.660.235.400.800', 'D12.776.664.235.400.800', 'D12.776.930.875'], ['A13.395.823'], ['D12.776.467.984.100', 'D12.776.624.664.700.967', 'D23.529.984.100']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
2,4-Dichlorophenoxyacetic acid influx is mediated by an active transport system in Chinese hamster ovary cells.
|
The influx of 2,4-dichlorophenoxyacetic acid (2,4-D) into Chinese hamster ovary (CHO) cells was studied. The cells mainly took up but did not metabolize the undissociated form of the herbicide. The uptake of 2,4-D was carried out against a concentration gradient and was inhibited by sodium azide and dinitrophenol. The results presented here show that the herbicide influx was an active, energy dependent process. (Na+ + K+)ATPase does not seem to be involved because ouabain, an inhibitor of the enzyme, did not affect the 2,4-D uptake.
|
['2,4-Dichlorophenoxyacetic Acid', '3-O-Methylglucose', 'Aminoisobutyric Acids', 'Animals', 'Azides', 'Biological Transport, Active', 'CHO Cells', 'Cricetinae', 'Culture Media, Conditioned', 'Dinitrophenols', 'Enzyme Inhibitors', 'Herbicides', 'Hydrogen-Ion Concentration', 'Kinetics', 'Methylglucosides', 'Ouabain', 'S Phase', 'Sodium Azide', 'Sodium-Potassium-Exchanging ATPase']
| 8,553,371
|
[['D02.241.081.018.386.682.224', 'D02.241.511.316.682.149'], ['D09.408.348.500.500', 'D09.408.514.622.500'], ['D02.241.081.114.968.249', 'D12.125.070.075', 'D12.125.190.055'], ['B01.050'], ['D01.625.100', 'D02.159'], ['G03.143.310'], ['A11.251.210.200', 'A11.436.155'], ['B01.050.150.900.649.313.992.635.075.250'], ['D27.720.470.305.250', 'E07.206.250'], ['D02.455.426.559.389.657.566.304', 'D02.640.743.304'], ['D27.505.519.389'], ['D27.720.031.700.366', 'D27.888.723.366'], ['G02.300'], ['G01.374.661', 'G02.111.490'], ['D09.408.348.500', 'D09.408.514.622'], ['D04.210.500.155.580.130.750.600', 'D09.408.180.810.600'], ['G02.111.225.880', 'G04.144.500.800', 'G05.226.880'], ['D01.625.100.750', 'D01.857.462'], ['D08.811.277.040.025.314.750', 'D12.776.157.530.450.162.780', 'D12.776.157.530.450.250.880', 'D12.776.157.530.813.750', 'D12.776.543.585.450.162.800', 'D12.776.543.585.450.250.890', 'D12.776.543.585.813.750']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Serration pattern analysis for differentiating epidermolysis bullosa acquisita from other pemphigoid diseases.
|
BACKGROUND: Direct immunofluorescence (DIF) microscopy of a skin biopsy specimen is the reference standard for the diagnosis of pemphigoid diseases (PDs). Serration pattern analysis enables the differentiation of epidermolysis bullosa acquisita (EBA) from other PDs using DIF microscopy alone. However, practice gaps need to be addressed in order to implement this technique in the routine diagnostic procedure.OBJECTIVE: We sought to determine and optimize the technical requirements for serration pattern analysis of DIF microscopy and determine interrater conformity of serration pattern analysis.METHODS: We compared serration pattern analysis of routine DIF microscopy from laboratories in Groningen, The Netherlands and L?beck, Germany with 4 blinded observers. Skin biopsy specimens from 20 patients with EBA and other PDs were exchanged and analyzed. Various factors were evaluated, including section thickness, transport medium, and biopsy specimen processing.RESULTS: The interrater conformity of our 4 observers was 95.7%. Recognition of serration patterns was comparable in samples transported in saline and in Michel's medium and with section thicknesses of 4, 6, and 8 ìm.LIMITATIONS: Limitations include our small sample size and the availability of 20 samples that were compared retrospectively.CONCLUSION: DIF serration pattern analysis is not restricted by variation in laboratory procedures, transport medium, or experience of observers. This learnable technique can be implemented as a routine diagnostic method as an extension of DIF microscopy for subtyping PD.
|
['Diagnosis, Differential', 'Epidermolysis Bullosa Acquisita', 'Humans', 'Microscopy, Fluorescence', 'Observer Variation', 'Pemphigoid, Bullous', 'Retrospective Studies']
| 29,154,993
|
[['E01.171'], ['C16.131.831.493.080', 'C17.800.804.493.080', 'C17.800.827.275.080', 'C17.800.865.410.080'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.515.458', 'E05.595.458'], ['E01.354.753', 'N02.421.450.600', 'N05.715.350.150.675', 'N06.850.490.500.250'], ['C17.800.865.690', 'C20.111.730'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Relationships between socioeconomic and lifestyle factors and indoor air quality in French dwellings.
|
BACKGROUND: To date, few studies have analyzed the relationships between socioeconomic status (SES) and indoor air quality (IAQ).OBJECTIVE: The aim of this study was to examine the relationships between socioeconomic and other factors and indoor air pollutant levels in French homes.METHODS: The indoor air concentrations of thirty chemical, biological and physical parameters were measured over one week in a sample of 567 dwellings representative of the French housing stock between September 2003 and December 2005. Information on SES (household structure, educational attainment, income, and occupation), building characteristics, and occupants' habits and activities (smoking, cooking, cleaning, etc.) were collected through administered questionnaires. Separate stepwise linear regression models were fitted to log-transformed concentrations on SES and other factors. Logistic regression was performed on fungal contamination data.RESULTS: Households with lower income were more likely to have higher indoor concentrations of formaldehyde, but lower perchloroethylene indoor concentrations. Formaldehyde indoor concentrations were also associated with newly built buildings. Smoking was associated with increasing acetaldehyde and PM2.5 levels and the risk of a positive fungal contamination index. BTEX levels were also associated with occupant density and having an attached garage. The major predictors for fungal contamination were dampness and absolute humidity.CONCLUSION: These results, obtained from a large sample of dwellings, show for the first time in France the relationships between SES factors and indoor air pollutants, and believe they should be considered alongside occupant activities and building characteristics when study IAQ in homes.
|
['Air Pollution, Indoor', 'Aldehydes', 'France', 'Humans', 'Life Style', 'Socioeconomic Factors', 'Volatile Organic Compounds']
| 25,935,319
|
[['N06.850.460.100.080'], ['D02.047'], ['Z01.542.286'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.829.458'], ['I01.880.853.996', 'N01.824'], ['D02.974']]
|
['Health Care [N]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
|
Resistance to medical therapy of gastric ulcers in rheumatic disease patients taking aspirin. A double-blind study with cimetidine and follow-up.
|
Little is known about healing or recurrence of aspirin-induced gastric ulcers if aspirin intake is continued. A double-blind controlled study compared cimetidine plus antacids as needed (prn) with placebo plus prn antacids in healing aspirin-associated gastric ulcers during continued salicylate ingestion in 18 rheumatic disease patients over a 2-month period. Healing occurred in 44% of the placebo and 56% of the cimetidine-treated patients (P greater than 0.05). Subjects in both groups ingested potentially therapeutic doses of antacid. Ulcer size had an effect on healing rate, irrespective of treatment. Ninety percent of gastric ulcers less than 0.5 cm in diameter healed in 2 months but only 25% of ulcers greater than 0.5 cm. Six of seven patients with unhealed ulcers at 2 months eventually healed medically at intervals of 6--26 months. Of 11 patients managed medically and followed endoscopically for a mean of 15 months after healing, only one had a recurrent ulcer. In conclusion, placebo and antacid therapy were as effective as cimetidine and antacids in healing ulcers over a 2-month period. In spite of continued aspirin intake, most benign gastric ulcers less than 0.5 cm in diameter heal medically in two months. Aspirin-induced ulcers greater than or equal to 1 cm in diameter are relatively resistant to therapy but can be healed with prolonged cimetidine and antacid treatment; once healed, recurrence rate is low with prophylactic therapy even with continued aspirin intake.
|
['Antacids', 'Aspirin', 'Cimetidine', 'Double-Blind Method', 'Follow-Up Studies', 'Guanidines', 'Humans', 'Placebos', 'Prospective Studies', 'Rheumatic Heart Disease', 'Stomach Ulcer']
| 7,140,494
|
[['D27.505.519.170', 'D27.505.954.483.080'], ['D02.455.426.559.389.657.410.595.176'], ['D02.078.370.200', 'D03.383.129.308.130'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['D02.078.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D26.660', 'E02.785'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['C01.150.252.410.890.731.649', 'C14.280.874'], ['C06.405.469.275.800.849', 'C06.405.748.586.849']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Pharmacokinetic and pharmacodynamic properties of batifiban coadministered with antithrombin agents in Chinese healthy volunteers.
|
The combined use of batifiban, a synthetic platelet GPII b/ IIIa receptor antagonist, and antithrombin agents is an attractive option for the treatment of patients with non-ST-segment elevation (NSTE) acute coronary syndrome (ACS) and those scheduled for percutaneous coronary intervention. To observe whether antithrombin agents affect the pharmacokinetic and pharmacodynamic properties of batifiban in combination therapy and optimize clinical administration dosage of batifiban, an open-label and parallel study was conducted. Thirty healthy subjects were randomly divided into three groups, which were sequentially treated with batifiban alone, or oral coadministration of clopidogrel, aspirin and UFH, or batifiban coadministered with these antithrombin agents. Blood samples were collected at pre-specified time points. The evaluation index included the inhibition of platelet aggregation and pharmacokinetic parameters. The pharmacokinetic parameters of batifiban and batifiban coadministered with antithrombin agents showed no significant differences. The mean inhibition rate of platelet aggregation (%) suggested that neither batifiban alone nor antithrombin agents alone could provide such potent inhibition rate (>80%) to obtain the best clinical efficacy, but they had a synergistic effect on platelet inhibition. No serious adverse effects were observed. The results in these healthy subjects suggest that batifiban coadministrated with antithrombin agents could achieve optimum clinical treatment effect for patients with NSTE ACS, and also those scheduled for percutaneous coronary intervention.
|
['Administration, Oral', 'Adolescent', 'Adult', 'Area Under Curve', 'Aspirin', 'China', 'Clopidogrel', 'Drug Administration Schedule', 'Female', 'Fibrinolytic Agents', 'Heparin', 'Humans', 'Infusions, Intravenous', 'Injections, Intravenous', 'Male', 'Metabolic Clearance Rate', 'Peptides, Cyclic', 'Platelet Aggregation Inhibitors', 'Ticlopidine', 'Time Factors', 'Young Adult']
| 24,142,738
|
[['E02.319.267.100'], ['M01.060.057'], ['M01.060.116'], ['E05.318.740.200', 'G03.787.101', 'G07.690.725.064', 'N06.850.520.830.200'], ['D02.455.426.559.389.657.410.595.176'], ['Z01.252.474.164'], ['D02.886.778.823.500.500', 'D03.383.725.849.500.500', 'D03.383.903.830.500.500', 'D03.633.100.928.500.500'], ['E02.319.283'], ['D27.505.519.421.750', 'D27.505.954.411.320', 'D27.505.954.502.427'], ['D09.698.373.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.319.267.082.500', 'E02.319.267.510.590'], ['E02.319.267.082.750', 'E02.319.267.530.540'], ['E01.370.225.843', 'E05.200.843', 'G03.490', 'G07.690.595', 'G07.690.725.513'], ['D04.345.566', 'D12.644.641'], ['D27.505.954.502.780'], ['D02.886.778.823.500', 'D03.383.725.849.500', 'D03.383.903.830.500', 'D03.633.100.928.500'], ['G01.910.857'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Comprehensive 3D-QSAR and binding mode of BACE-1 inhibitors using R-group search and molecular docking.
|
The â-enzyme (BACE), which takes an active part in the processing of amyloid precursor protein, thereby leads to the production of amyloid-â (Aâ) in the brain, is a major therapeutic target against Alzheimer's disease. The present study is aimed at studying 3D-QSAR of BACE-1 inhibitors and their binding mode. We build a 3D-QSAR model involving 99 training BACE-1 inhibitors based on Topomer CoMFA, and 26 molecules are employed to validate the external predictive power of the model obtained. The multiple correlation coefficients of fitting modeling, leave one out cross validation, and external validation are 0.966, 0.767 and 0.784, respectively. Topomer search is used as a tool for virtual screening in lead-like compounds of ZINC databases (2012); as a result, we successfully design 30 new molecules with higher activity than that of all training and test inhibitors. Besides, Surflex-dock is employed to explore binding mode of the inhibitors studied when acting with BACE-1 enzyme. The result shows that the inhibitors closely interact with the key sites related to ASP93, THR133, GLN134, ASP289, GLY291, THR292, THR293, ASN294, ARG296 and SER386 of BACE-1.
|
['Amyloid Precursor Protein Secretases', 'Aspartic Acid Endopeptidases', 'Binding Sites', 'Drug Design', 'Humans', 'Hydrogen Bonding', 'Models, Molecular', 'Molecular Docking Simulation', 'Molecular Structure', 'Protease Inhibitors', 'Protein Binding', 'Quantitative Structure-Activity Relationship']
| 24,004,830
|
[['D08.811.277.656.300.032'], ['D08.811.277.656.074.500', 'D08.811.277.656.300.048'], ['G02.111.570.120'], ['E05.290.500', 'H01.158.703.007.338.500', 'H01.181.466.338.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.282'], ['E05.599.595'], ['E05.599.595.249', 'L01.224.160.249'], ['G02.111.570', 'G02.466'], ['D27.505.519.389.745'], ['G02.111.679', 'G03.808'], ['G02.111.830.500', 'G07.690.773.997.500']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Information Science [L]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Asymmetric retraction of growth cone filopodia following focal inactivation of calcineurin.
|
The neuronal growth cone is thought to be the site of decision making in nerve growth and guidance. One likely mechanism of how the growth cone translates various extracellular cues into directed motility involves rises in intracellular calcium. A variety of physiological cues, such as adhesion molecules and neurotransmitters, increases intracellular calcium, and artificial manipulations of growth cone calcium levels affect growth cone morphology and neurite outgrowth. The molecular events downstream of calcium fluxes are incompletely understood. Here we show that calcineurin, a protein phosphatase enriched in growth cones that is dependent on calcium ions and calmodulin, functions in neurite outgrowth and directed filopodial motility in cultured chick dorsal root ganglia neurons. Cyclosporin A and FK506, inhibitors of calcineurin, delayed neuritogenesis and inhibited neurite extension. Chromophore-assisted laser inactivation of calcineurin in regions of growth cones causes localized filopodial and lamellipodial retraction and influences the direction of subsequent outgrowth. We suggest that a spatial distribution of calcineurin activity within the growth cone can regulate motility and direct outgrowth.
|
['Animals', 'Calcineurin', 'Calmodulin-Binding Proteins', 'Cattle', 'Cell Division', 'Cell Movement', 'Cells, Cultured', 'Chick Embryo', 'Cyclosporine', 'Ganglia, Spinal', 'Lasers', 'Neurites', 'Phosphoprotein Phosphatases', 'Polyenes', 'Rosaniline Dyes', 'Signal Transduction', 'Sirolimus', 'Tacrolimus']
| 7,544,441
|
[['B01.050'], ['D08.811.277.352.650.625.150', 'D12.644.360.087', 'D12.776.476.087'], ['D12.776.157.142'], ['B01.050.150.900.649.313.500.380.271'], ['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['G04.198', 'G07.568.500.180'], ['A11.251'], ['A13.350.150', 'A16.331.200'], ['D04.345.566.235.300', 'D12.644.641.235.300'], ['A08.340.390.340', 'A08.800.350.340', 'A08.800.800.720.725.350'], ['E07.632.490', 'E07.710.520'], ['A08.675.256.500', 'A08.675.542.145.500', 'A11.284.180.610', 'A11.671.501.145.500', 'A11.671.543'], ['D08.811.277.352.650.625'], ['D02.455.326.271.665'], ['D02.092.146.755'], ['G02.111.820', 'G04.835'], ['D02.540.505.760'], ['D02.540.505.810']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Neonatology nurses' knowledge about Peripherally Inserted Central Venous Catheter].
|
The Peripherally Inserted Central Catheter (PICC) has been used as a safe venous access for infants at risk. The aim of this study was to describe the knowledge and practice of nurses from the five public Neonatal Intensive Care Units, of Recife-PE, Brazil, about the use of the PICC. The sample was comprised by 52 nurses; data were collected from January to February/2010. It was found that 64,8% of nurses did not have license for insertion of the PICC. Only two units routinely used the PICC. About the indication of the access, the accuracy was above 70%. In unit B only 8,3% of nurses reported adequate initial location of the catheter tip. It was concluded that is necessary greater incentives to train nurses to use the PICC.
|
['Adult', 'Catheterization, Peripheral', 'Central Venous Catheters', 'Clinical Competence', 'Female', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Infant, Newborn', 'Intensive Care Units, Neonatal', 'Male', 'Neonatal Nursing']
| 22,751,707
|
[['M01.060.116'], ['E02.148.224', 'E04.100.814.529.937', 'E04.502.382.937', 'E05.157.375'], ['E07.132.750.500'], ['I02.399.630.210', 'N04.761.210', 'N05.715.175'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['N02.278.388.493.390.380'], ['H02.478.676.390.600', 'H02.478.676.631.600', 'N02.421.533.460.600', 'N02.421.533.691.600']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
|
Competition between two virulent Marek's disease virus strains in vivo.
|
Previous studies have demonstrated the presence of multiple strains of Marek's disease virus simultaneously circulating within poultry flocks, leading to the assumption that individual birds are repeatedly exposed to a variety of virus strains in their lifetime. Virus competition within individual birds may be an important factor that influences the outcome of co-infection under field conditions, including the potential outcome of emergence or evolution of more virulent strains. A series of experiments was designed to evaluate virus competition within chickens following simultaneous challenge with two virulent serotype 1 Marek's disease virus strains, using either pathogenically similar (rMd5 and rMd5/pp38CVI) or dissimilar (JM/102W and rMd5/pp38CVI) virus pairs. Bursa of Fabricius, feather follicle epithelium, spleen, and tumour samples were collected at multiple time points to determine the frequency and distribution of each virus present using pyrosequencing, immunohistochemistry and virus isolation. In the similar pair, rMd5 appeared to have a competitive advantage over rMd5/pp38CVI, which in turn had a competitive advantage over the less virulent JM/102W in the dissimilar virus pair. Dominance of one strain over the other was not absolute for either virus pair, as the subordinate virus was rarely eliminated. Interestingly, competition between two viruses with either pair rarely ended in a draw. Further work is needed to identify factors that influence virus-specific dominance to better understand what characteristics favour emergence of one strain in chicken populations at the expense of other strains.
|
['Animals', 'Antibodies, Monoclonal', 'Chickens', 'Coinfection', 'Herpesvirus 2, Gallid', 'Immunohistochemistry', 'Marek Disease', 'Microbial Interactions', 'Population Dynamics', 'Sequence Analysis, DNA', 'Species Specificity', 'Statistics, Nonparametric', 'Virulence']
| 22,702,454
|
[['B01.050'], ['D12.776.124.486.485.114.224', 'D12.776.124.790.651.114.224', 'D12.776.377.715.548.114.224'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['C01.218'], ['B04.280.382.100.562.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['C01.925.256.466.650', 'C01.925.928.489', 'C15.604.515.700', 'C20.683.515.840', 'C22.131.546'], ['G06.550'], ['I01.240.600', 'N01.224.625', 'N06.850.505.400.700'], ['E05.393.760.700'], ['G16.824'], ['E05.318.740.995', 'N05.715.360.750.760', 'N06.850.520.830.995'], ['G06.930']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
|
Effect of soil pH on as hyperaccumulation capacity in fern species, Pityrogramma calomelanos.
|
Arsenic uptake by hyperaccumulator plant species depends on many different environmental factors. Soil pH is one of the most important factors due to its combined effect on both chemical and biological processes. In greenhouse experiment, the effect of pH (within the pH range 3.6 - 8.9) on As uptake as well as biomass of Pityrogramma calomelanos was evaluated. The plants were grown in mining soil containing 645.6 mg As kg(-1) for 14 weeks. Within this time, the plant biomass growth was 3.78 - 8.64 g d. wt. per plant and the removal amounted 6.3-18.4 mg As per plant. Translocation factor (ratio of As in fronds to roots) of the fern was 3.6 - 9.7, indicating its potential in phytoremediation of As contaminated soil. Influence of pH on As bioavailability was visible as the available As concentration was higher in acidic soil compared to alkaline soil. Furthermore, it was found that As accumulation by Pityrogramma calomelanos was optimum in the soil of pH 3.6. Nevertheless, the results of this study demonstrate that remediation of As-contaminated mining soils, by this fern, can be improved by changing the soil pH from 4.6 to 6.8.
|
['Arsenic', 'Biodegradation, Environmental', 'Ferns', 'Hydrogen-Ion Concentration', 'Mining', 'Soil']
| 24,620,585
|
[['D01.268.513.249'], ['N06.230.080.600.500', 'N06.850.460.375.500'], ['B01.650.940.800.575.912.063'], ['G02.300'], ['J01.576.655.875.500'], ['D20.721', 'G01.311.820', 'G16.500.275.815', 'N06.230.600']]
|
['Chemicals and Drugs [D]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
A complex pattern of disposition of phenytoin in severe intoxication.
|
A 5-year-old child developed phenytoin (diphenylhydantoin, DPH) toxicity after receiving 500 mg of the drug daily for 3 weeks. Plasma, urine, and duodenal fluid were collected for assay of DPH and its metabolites. The peak plasma concentration of DPH was 108 mug/ml, and the decline in plasma level did not fit first-order kinetics. The para-hydroxy, meta-hydroxy, and dihydrodiol metabolites of DPH were measured in urine; duodenal aspirate contained both DPH and the para-hydroxy metabolite. Plasma pH may affect distribution of DPH since in vitro binding of DPH to human albumin increased as pH increased.
|
['Child, Preschool', 'Duodenum', 'Glucuronates', 'Humans', 'Hydrogen-Ion Concentration', 'Kinetics', 'Male', 'Medication Errors', 'Phenytoin', 'Protein Binding', 'Seizures', 'Serum Albumin', 'Solubility', 'Time Factors']
| 238,781
|
[['M01.060.406.448'], ['A03.556.124.684.124', 'A03.556.875.249'], ['D02.241.081.844.915.162', 'D02.241.152.811.162', 'D02.241.511.902.915.162', 'D09.811.922.162'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['G01.374.661', 'G02.111.490'], ['E02.319.529', 'N02.421.450.500'], ['D03.383.129.308.432.555.730'], ['G02.111.679', 'G03.808'], ['C10.597.742', 'C23.888.592.742'], ['D12.776.034.841', 'D12.776.124.727'], ['G02.805'], ['G01.910.857']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Differences in pain knowledge in cancer patients with and without pain.
|
PURPOSE: The authors determined: 1) whether there were differences in knowledge about pain between oncology outpatients with and without cancer-related pain and 2) whether there were relationships between selected patient characteristics (age, gender, education, Karnofsky performance status, pain intensity, and pain duration) and the knowledge about pain of oncology outpatients with cancer-related pain.DESCRIPTION OF STUDY: Three hundred sixty-nine oncology outpatients completed several self-report questionnaires including a demographic questionnaire, a pain experience scale that measured knowledge about pain and pain management, the Karnofsky performance scale, and descriptive numeric rating scales that measured pain intensity and pain duration.RESULTS: Patients with cancer-related pain knew significantly more about pain and its management than pain-free patients (P < 0.004). However, in both groups, mean knowledge scores were below 60%. Older patients with cancer-related pain had less knowledge about pain than younger patients (P < 0.0001). In addition, patients with cancer pain who had more education and those with higher reported pain intensity scores had more knowledge about pain and pain management. Finally, women with cancer pain had more knowledge than men about pain and pain management.CLINICAL IMPLICATIONS: Results of this study suggest that oncology patients with and without pain need more education about pain and effective pain management strategies.
|
['Adult', 'Educational Measurement', 'Female', 'Humans', 'Male', 'Middle Aged', 'Neoplasms', 'Outpatients', 'Pain', 'Patient Education as Topic', 'Retrospective Studies', 'Surveys and Questionnaires']
| 9,128,495
|
[['M01.060.116'], ['I02.399'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C04'], ['M01.643.630'], ['C23.888.592.612', 'F02.830.816.444', 'G11.561.790.444'], ['I02.233.332.500', 'N02.421.726.407.680'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Mammalian cAMP-responsive element can activate transcription in yeast and binds a yeast factor(s) that resembles the mammalian transcription factor ANF.
|
The human ATF and AP1 transcription factors bind to highly related DNA sequences. Their consensus binding sites differ by a single nucleotide, but this single change is crucial in determining factor binding specificity. We have previously identified an AP1 (yAP1) binding activity in yeast. In this report we identify a yeast ATF (yATF) binding activity whose specificity can be distinguished from that of yAP1 by the same crucial nucleotide that distinguishes binding of human ATF and AP1. The ATF binding site can act as an efficient upstream activating sequence in vivo, suggesting that yATF is a transcriptional activator. The yATF DNA-binding complex is phosphorylated and the binding activity of partially purified yATF can be enhanced in vitro by the addition of protein kinase A, indicating that the phosphorylation state of yATF may be important in determining its ability to bind DNA.
|
['Activating Transcription Factors', 'Adenoviruses, Human', 'Alkaline Phosphatase', 'Base Sequence', 'Blood Proteins', 'Cell Nucleus', 'Cyclic AMP', 'Gene Expression Regulation', 'Genes, Fungal', 'HeLa Cells', 'Humans', 'Molecular Sequence Data', 'Phosphorylation', 'Promoter Regions, Genetic', 'Protein Kinases', 'Saccharomyces cerevisiae', 'Schizosaccharomyces', 'Transcription Factors', 'Transcription, Genetic']
| 2,538,834
|
[['D12.776.260.108.061', 'D12.776.930.127.061'], ['B04.280.030.500.350'], ['D08.811.277.352.650.035'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D12.776.124'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['D03.633.100.759.646.138.395', 'D13.695.462.200', 'D13.695.667.138.395', 'D13.695.827.068.395'], ['G05.308'], ['G05.360.340.024.340.364.500', 'G05.360.340.358.024.500', 'G05.360.340.358.365.500'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453.245.667'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['D08.811.913.696.620.682'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['B01.300.107.797', 'B01.300.930.720'], ['D12.776.930'], ['G02.111.873', 'G05.297.700']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Uptake of aqueous and dietary metals by mussel Perna viridis with different Cd exposure histories.
|
The influences of different Cd pre-exposure regimes (route, concentration, and duration of Cd exposure) on the bioavailability of Cd, Ag, Hg, and Zn to the green mussels Perna viridis were quantified in this study. Following pre-exposing the mussels to Cd, we measured the mussel's tissue Cd concentration and clearance rate, as well as the metal dietary assimilation efficiency (AE) and the influx rate from the dissolved phase of the four studied metals. Differences in the route (aqueous and dietary pathways) and the history of pre-exposure (combined Cd concentration and duration) did not significantly affect the subsequent Cd dietary and aqueous uptake. The Cd dietary AEs increased following both the dissolved and dietary Cd pre-exposure. There was a significant correlation between the Cd AE and the accumulated Cd body concentration in the mussels. Dietary assimilation of Hg and Zn also increased slightly (but not significantly) after Cd pre-exposure, but the AEs of Ag remained constant. Except for the significant decrease in the dissolved uptake of Hg, Cd pre-exposure did not apparently affect the uptake of the other three metals from the solution. Metal-metal interactions are likely to be affected by the specificity of metallothionein induction. Our study demonstrated that the Cd body concentration as well as the environmental Cd concentration instead of the history of pre-exposure was more important in affecting the Cd accumulation in the mussels. Such factors need to be considered in interpreting metal body concentrations in biomonitors.
|
['Animals', 'Biological Availability', 'Bivalvia', 'Body Burden', 'Cadmium', 'Metals', 'Tissue Distribution']
| 16,382,965
|
[['B01.050'], ['G03.787.151', 'G07.690.725.129'], ['B01.050.500.644.080'], ['E05.799.638.231', 'N06.850.460.200'], ['D01.268.556.137', 'D01.268.956.061', 'D01.552.544.137'], ['D01.552'], ['G03.787.917', 'G07.690.725.949']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
[Low T3 syndrome and left ventricular diastolic function].
|
BACKGROUND: Recent data suggest that low triiodothyronine (T3) syndrome may contribute to the pathophysiology of cardiac diseases. Because the development of diastolic dysfunction occurs early in a failing heart, we evaluated whether patients with low T3 syndrome show abnormalities in diastolic function, also in absence of overt cardiovascular disease.METHODS: Thirty-four patients with low T3 syndrome and 34 controls with normal levels of free T3 (FT3) underwent a complete Doppler echocardiographic examination. Criteria of exclusion from the study were the presence of cardiovascular disease or traditional cardiovascular risk factors, a primitive thyroid disease, cachexia, and the use of drugs affecting the thyroid function.RESULTS: Patients with low T3 syndrome, if compared with controls, show evidence of left ventricular diastolic dysfunction, documented by prolongation of the isovolumic relaxation time (120 vs 75 ms, p < 0.0001) and a reduction in the early diastolic mitral flow velocity/late diastolic mitral flow velocity ratio (0.66 vs 2.05, p < 0.0001), mainly due to the increased A-wave (0.96 vs 0.40 m/s, p < 0.0001). These alterations increase proportionally with FT3 reduction.CONCLUSIONS: Patients with low T3 syndrome show evidence of impaired left ventricular relaxation. Doppler echocardiography may be a useful non-invasive technique for the assessment of diastolic performance in these patients.
|
['Aged', 'Biomarkers', 'Case-Control Studies', 'Diastole', 'Echocardiography, Doppler', 'Euthyroid Sick Syndromes', 'Female', 'Heart Diseases', 'Humans', 'Male', 'Middle Aged', 'Predictive Value of Tests', 'Retrospective Studies', 'Thyrotropin', 'Thyroxine', 'Triiodothyronine', 'Ventricular Dysfunction, Left', 'Ventricular Function, Left']
| 19,771,751
|
[['M01.060.116.100'], ['D23.101'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['G09.330.580.295', 'G11.427.494.554.250', 'G11.427.494.570.295'], ['E01.370.350.130.750.220', 'E01.370.350.850.220.220', 'E01.370.350.850.850.220', 'E01.370.370.380.220.220'], ['C19.874.255'], ['C14.280'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['D06.472.699.631.525.883', 'D12.644.548.691.525.883'], ['D06.472.931.812', 'D12.125.072.050.767'], ['D06.472.931.740.385', 'D12.125.072.050.767.741.894'], ['C14.280.945.900'], ['G09.330.955.800']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Highly sensitive two-site immunoradiometric assay of parathyrin, and its clinical utility in evaluating patients with hypercalcemia.
|
We have developed a highly sensitive, two-site immunoradiometric assay (IRMA) for human parathyrin (PTH) that is specific for the intact, secreted, biologically active 84-amino-acid peptide. This assay has several technical advantages: it does not detect even high concentrations of inactive carboxyl-terminal fragments, results are available within 24 h, and the detection limit for intact hormone is low (1 ng/L). The assay readily measures concentrations of PTH in all healthy subjects and distinguishes these values from low or undetectable PTH values observed in clinical situations in which PTH secretion is expected to be suppressed. We found complete separation of results from 37 patients with surgically proven hyperparathyroidism and those from 23 patients with hypercalcemia associated with malignancy, the latter having PTH values at or below the lower limits of normal for this assay. The sensitivity, specificity, and rapid turnaround time of this two-site IRMA should advance the laboratory evaluation of patients with disorders of calcium metabolism.
|
['Humans', 'Hypercalcemia', 'Hyperparathyroidism', 'Indicators and Reagents', 'Neoplasms', 'Parathyroid Hormone', 'Radioimmunoassay', 'Reference Values']
| 3,608,153
|
[['B01.050.150.900.649.313.988.400.112.400.400'], ['C18.452.174.451', 'C18.452.950.340'], ['C19.642.355'], ['D27.720.470.410'], ['C04'], ['D06.472.699.590', 'D12.644.548.587'], ['E01.370.384.700', 'E05.478.566.639', 'E05.601.470.639'], ['E05.978.810']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Influence of gamma-aminobutyric acid on lordosis behavior and dopamine activity in estrogen primed spayed female rats.
|
In the first experiment the role of gamma-aminobutyric acid (GABA) in the display of lordosis behavior was examined in septal-lesioned and sham-operated ovariectomized rats. Following estradiol benzoate (EB) priming, septal-lesioned rats were tested for lordosis behavior before and after bilateral infusion of picrotoxin or saline directly into the substantia nigra (SN). Sham animals were given the same behavioral tests but received intranigral infusion of either hydrazinopropionic acid (HPA) or saline. Picrotoxin, which blocks GABA receptors, was effective in suppressing the high levels of lordosis behavior seen in the EB-primed septal-lesioned female rat 30 min after infusion, but not at 120 min. Conversely, HPA, which elevates endogenous GABA levels, was effective in facilitating lordosis behavior in sham-operated rats treated with EB only. The lordosis quotient was moderately increased 30 min after HPA infusion, reached high levels at 120 min, and returned to low levels by 360 min post-infusion, demonstating the reversibility of the drug effect. Saline infusions in lesioned and sham-operated controls were without effect. In the second experiment sepal-lesioned and sham-operated rats were primed with EB and infused with the drugs as in the first experiment, but were sacrificed at the time the macimal behavioral effect had been observed in the first experiment. Tyrosine hydroxylase (TH) activity and dopamine (DA) and homovanillic acid (HVA) levels were measured. No effect on TH activity was found. However, sham-operated rats receiving HPA infusions had lower DA and HVA levels compared to those of saline-injected controls, and septal-lesioned rats receiving picrotoxin infusions had higher DA and HVA levels than those of lesioned saline-injected controls. Septal-lesioned saline-infused rats also showed decreased DA and HVA levels relative to sham-operated saline-infused animals. These results support the concept of a GABA inhibitory neuronal feedback system which modulates DA turnover and perhaps plays a critical role in the neural control of lordosis behavior.
|
['Animals', 'Castration', 'Dopamine', 'Estradiol', 'Female', 'Muscimol', 'Picrotoxin', 'Propionates', 'Rats', 'Septal Nuclei', 'Sexual Behavior, Animal', 'Substantia Nigra', 'gamma-Aminobutyric Acid']
| 7,357,416
|
[['B01.050'], ['E04.270.282', 'E04.950.165'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['D04.210.500.365.415.248', 'D06.472.334.851.437.500'], ['D03.383.129.462.470', 'D23.946.587.587'], ['D02.455.426.392.368.367.800', 'D02.540.552'], ['D02.241.081.751', 'D10.251.400.706'], ['B01.050.150.900.649.313.992.635.505.700'], ['A08.186.211.180.750.800', 'A08.186.211.200.885.750.800'], ['F01.145.113.252.748'], ['A08.186.211.132.659.413.656'], ['D02.241.081.114.500.350', 'D12.125.190.350']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Genetic aspects of male infertility].
|
We examined 118 men with infertility. Among them we identified phenotypic syndromes associated with infertility in 4 and chromosomal abnormalities in 16. Further molecular genetic study of 98 infertile men found that microdeletions in AZFc-locus had 3, pathological AR allele had 2, CFTR gene mutation had 4 of them. In 37 infertile men an increased DNA fragmentation index (>20%) was found.
|
['Chromosome Aberrations', 'Chromosomes, Human, Y', 'Cystic Fibrosis Transmembrane Conductance Regulator', 'DNA Fragmentation', 'Female', 'Humans', 'Infertility, Male', 'Kartagener Syndrome', 'Karyotyping', 'Male', 'Mutation', 'Oligospermia', 'Pedigree', 'Receptors, Androgen']
| 24,850,600
|
[['C23.550.210', 'G05.365.590.175'], ['A11.284.187.520.300.505.757', 'A11.284.187.865.983.500', 'G05.360.162.520.300.505.757', 'G05.360.162.865.983.500'], ['D12.776.157.530.100.304.500', 'D12.776.157.530.400.175.125', 'D12.776.157.530.450.074.500.500.500.500', 'D12.776.543.550.450.175.125', 'D12.776.543.585.100.304.500', 'D12.776.543.585.400.175.125', 'D12.776.543.585.450.074.500.500.500.500'], ['G05.200.230'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C12.294.365.700'], ['C08.127.384.500', 'C08.200.531', 'C08.695.501', 'C09.150.531', 'C14.240.400.280.500', 'C14.280.400.280.500', 'C16.131.077.245.500.531', 'C16.131.240.400.280.500', 'C16.131.740.501', 'C16.131.810.250.500', 'C16.320.184.500.531', 'C16.320.480'], ['E01.370.225.500.385.315', 'E05.200.500.385.315', 'E05.242.385.315', 'E05.393.285.475'], ['G05.365.590'], ['C12.294.365.700.508'], ['E05.393.673'], ['D12.776.826.750.150']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Achievement of recommended glucose and blood pressure targets in patients with type 2 diabetes and hypertension in clinical practice - study rationale and protocol of DIALOGUE.
|
BACKGROUND: Patients with type 2 diabetes have 2-4 times greater risk for cardiovascular morbidity and mortality than those without, and this is even further aggravated if they also suffer from hypertension. Unfortunately, less than one third of hypertensive diabetic patients meet blood pressure targets, and more than half fail to achieve target HbA1c values. Thus, appropriate blood pressure and glucose control are of utmost importance. Since treatment sometimes fails in clinical practice while clinical trials generally suggest good efficacy, data from daily clinical practice, especially with regard to the use of newly developed anti-diabetic and anti-hypertensive compounds in unselected patient populations, are essential. The DIALOGUE registry aims to close this important gap by evaluating different treatment approaches in hypertensive type 2 diabetic patients with respect to their effectiveness and tolerability and their impact on outcomes. In addition, DIALOGUE is the first registry to determine treatment success based on the new individualized treatment targets recommended by the ADA and the EASD.METHODS: DIALOGUE is a prospective observational German multicentre registry and will enrol 10,000 patients with both diabetes and hypertension in up to 700 sites. After a baseline visit, further documentations are scheduled at 6, 12 and 24 months. There are two co-primary objectives referring to the most recent guidelines for the treatment of diabetes and hypertension: 1) individual HbA1c goal achievement with respect to anti-diabetic pharmacotherapy and 2) individual blood pressure goal achievement with different antihypertensive treatments. Among the secondary objectives the rate of major cardio-vascular and cerebro-vascular events (MACCE) and the rate of hospitalizations are the most important.CONCLUSION: The registry will be able to gain insights into the reasons for the obvious gap between the demonstrated efficacy and safety of anti-diabetic and anti-hypertensive drugs in clinical trials and their real world balance of effectiveness and safety.
|
['Antihypertensive Agents', 'Biomarkers', 'Blood Glucose', 'Blood Pressure', 'Cardiovascular Diseases', 'Cerebrovascular Disorders', 'Diabetes Mellitus, Type 2', 'Germany', 'Glycated Hemoglobin A', 'Guideline Adherence', 'Humans', 'Hypertension', 'Hypoglycemic Agents', 'Practice Guidelines as Topic', "Practice Patterns, Physicians'", 'Prospective Studies', 'Registries', 'Research Design', 'Time Factors', 'Treatment Outcome']
| 23,216,660
|
[['D27.505.954.411.162'], ['D23.101'], ['D09.947.875.359.448.500'], ['E01.370.600.875.249', 'G09.330.380.076'], ['C14'], ['C10.228.140.300', 'C14.907.253'], ['C18.452.394.750.149', 'C19.246.300'], ['Z01.542.315'], ['D09.400.430.937', 'D12.776.124.400.405.440', 'D12.776.395.381', 'D12.776.422.316.762.380.440'], ['N04.761.337', 'N05.715.360.395'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C14.907.489'], ['D27.505.696.422'], ['N04.761.700.350.650', 'N05.700.350.650'], ['N04.590.374.577', 'N05.300.625'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.308.970', 'N04.452.859.819', 'N05.715.360.300.715.700', 'N06.850.520.308.970'], ['E05.581.500', 'H01.770.644.728'], ['G01.910.857'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Geographicals [Z]', 'Health Care [N]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
|
Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers.
|
During cancer progression, malignant cells undergo epithelial-mesenchymal transitions (EMT) and mesenchymal-epithelial transitions (MET) as part of a broad invasion and metastasis program. We previously observed MET events among lung metastases in a preclinical model of prostate adenocarcinoma that suggested a relationship between epithelial plasticity and metastatic spread. We thus sought to translate these findings into clinical evidence by examining the existence of EMT in circulating tumor cells (CTC) from patients with progressive metastatic solid tumors, with a focus on men with castration-resistant prostate cancer (CRPC) and women with metastatic breast cancer. We showed that the majority (> 80%) of these CTCs in patients with metastatic CRPC coexpress epithelial proteins such as epithelial cell adhesion molecule (EpCAM), cytokeratins (CK), and E-cadherin, with mesenchymal proteins including vimentin, N-cadherin and O-cadherin, and the stem cell marker CD133. Equally, we found that more than 75% of CTCs from women with metastatic breast cancer coexpress CK, vimentin, and N-cadherin. The existence and high frequency of these CTCs coexpressing epithelial, mesenchymal, and stem cell markers in patients with progressive metastases has important implications for the application and interpretation of approved methods to detect CTCs.
|
['Biomarkers, Tumor', 'Breast Neoplasms', 'Epithelial-Mesenchymal Transition', 'Female', 'Humans', 'Male', 'Neoplasm Staging', 'Neoplastic Cells, Circulating', 'Prostatic Neoplasms']
| 21,665,936
|
[['D23.101.140'], ['C04.588.180', 'C17.800.090.500'], ['G04.356.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.789.625'], ['A11.642', 'C04.697.650.900', 'C23.550.727.650.900'], ['C04.588.945.440.770', 'C12.294.260.750', 'C12.294.565.625', 'C12.758.409.750']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Intravaginal energy-based devices and sexual health of female cancer survivors: a systematic review and meta-analysis.
|
A systematic review and meta-analysis was undertaken to assess the efficacy and safety of intravaginal energy-based therapies (laser and radiofrequency) on sexual health of cancer survivors (CS) (breast cancer (BCS) and/or gynecological cancer (GCS)). PubMed, Scopus, Web of Science, and Cochrane Library were searched until 21/02/2019. Quality of reporting, methodology, and body of evidence were assessed using STROBE, MINORS, and GRADE. Primary outcomes were dyspareunia, dryness, and sexual health (FSFI, FSDS-R). Secondary outcomes were burning, itching, dysuria, incontinence, Vaginal Health Index Score (VHIS), microbiome-cytokine evaluation, and adverse events. Main analyses, subgroup analyses, and sensitivity analyses were performed. Eight observational studies (n = 274) were eligible for inclusion. None of the studies evaluated radiofrequency. BCS and BCS-GCS were included in 87% and 13% of studies, respectively. All primary outcomes improved significantly with the exception of FSDS-R (dyspareunia (5 studies (n = 233), standardized mean difference (StdMD) (- 1.17), 95%CI [- 1.59, - 0.75]; p < 0.001; I2 = 55%), vaginal dryness (4 studies (n = 183), StdMD (- 1.98), 95%CI [- 3.31, - 0.65]; p = 0.003; I2 = 91%), FSFI (2 studies, n = 28, MD (12.79), 95%CI [7.69, 17.89]; p < 0.001; I2 = 0%). Itching, dysuria, and VHIS increased significantly, while burning was not improved. Serious adverse events were not observed by any of the studies. Intravaginal laser therapies appear to have a positive effect on dyspareunia, vaginal dryness, and FSFI of CS. However, the quality of evidence is "very low," with no data on intravaginal radiofrequency therapy. Further research with high-quality RCTs and long-term follow-up is needed to evaluate the value of energy-based devices as a therapeutic option for CS with sexual problems.
|
['Cancer Survivors', 'Dyspareunia', 'Female', 'Humans', 'Laser Therapy', 'Sexual Health', 'Vagina']
| 31,396,795
|
[['M01.860.350'], ['C12.294.644.242', 'C13.351.500.665.313', 'F03.835.199'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E02.594', 'E04.014.520'], ['N01.400.663'], ['A05.360.319.779']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Using uncertainty and sensitivity analyses in socioecological agent-based models to improve their analytical performance and policy relevance.
|
Agent-based models (ABMs) have been widely used to study socioecological systems. They are useful for studying such systems because of their ability to incorporate micro-level behaviors among interacting agents, and to understand emergent phenomena due to these interactions. However, ABMs are inherently stochastic and require proper handling of uncertainty. We propose a simulation framework based on quantitative uncertainty and sensitivity analyses to build parsimonious ABMs that serve two purposes: exploration of the outcome space to simulate low-probability but high-consequence events that may have significant policy implications, and explanation of model behavior to describe the system with higher accuracy. The proposed framework is applied to the problem of modeling farmland conservation resulting in land use change. We employ output variance decomposition based on quasi-random sampling of the input space and perform three computational experiments. First, we perform uncertainty analysis to improve model legitimacy, where the distribution of results informs us about the expected value that can be validated against independent data, and provides information on the variance around this mean as well as the extreme results. In our last two computational experiments, we employ sensitivity analysis to produce two simpler versions of the ABM. First, input space is reduced only to inputs that produced the variance of the initial ABM, resulting in a model with output distribution similar to the initial model. Second, we refine the value of the most influential input, producing a model that maintains the mean of the output of initial ABM but with less spread. These simplifications can be used to 1) efficiently explore model outcomes, including outliers that may be important considerations in the design of robust policies, and 2) conduct explanatory analysis that exposes the smallest number of inputs influencing the steady state of the modeled system.
|
['Agriculture', 'Computer Simulation', 'Conservation of Natural Resources', 'Ecosystem', 'Environmental Policy', 'Geography', 'Michigan', 'Models, Biological', 'Probability', 'Soil', 'Uncertainty']
| 25,340,764
|
[['J01.040'], ['L01.224.160'], ['J01.256', 'N06.230.080'], ['G16.500.275.157', 'N06.230.124'], ['I01.655.500.608.180', 'I01.880.604.825.608.180', 'N03.623.500.608.180', 'N06.230.204'], ['H01.277.500'], ['Z01.107.567.875.350.500', 'Z01.107.567.875.510.500'], ['E05.599.395'], ['E05.318.740.600', 'G17.680', 'N05.715.360.750.625', 'N06.850.520.830.600'], ['D20.721', 'G01.311.820', 'G16.500.275.815', 'N06.230.600'], ['E05.318.740.600.900', 'F02.463.785.373.820', 'G17.680.875', 'N05.715.360.750.625.850', 'N06.850.520.830.600.900']]
|
['Technology, Industry, and Agriculture [J]', 'Information Science [L]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Disciplines and Occupations [H]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]']
| 0
| 0
| 0
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
|
Prevalence and relationship of olfactory dysfunction and tinnitus among middle- and old-aged population in Korea.
|
Olfactory dysfunction and tinnitus are age-related otorhinolaryngological disorders with a high prevalence in the elderly population and share several common clinical features. However, there is no study investigating the relationship between these two diseases. We studied the prevalence of olfactory dysfunction and tinnitus among Koreans and studied the relationship between these two diseases based on the Korean National Health and Nutrition Examination Survey. The subjects of this study were enrolled from the Fifth Korean National Health and Nutrition Examination Survey (2010-2012, n = 25,534). Data of subjects aged 40 years and older who underwent physical examination and completed a self-reported questionnaire and other anthropometric variables were statistically analyzed. Odds ratios were calculated to identify the relationship between olfactory dysfunction and tinnitus, using multiple logistic regression models. Older males, non-smokers, non/lower alcohol drinker groups exhibited the relationship between olfactory dysfunction and tinnitus. Metabolic syndrome and mental health problems were associated with both olfactory dysfunction and tinnitus. After adjusting for confounding factors, olfactory dysfunction was significantly associated with tinnitus (OR 1.318). There was a dose-response relationship between tinnitus severity and the odds of olfactory dysfunction (ORs for mild, moderate and severe tinnitus were, respectively, 1.134, 1.569 and 2.044). Additional molecular genetics and animal studies are needed to determine the shared pathophysiology of the two diseases.
|
['Alcohol Drinking', 'Female', 'Humans', 'Male', 'Mental Health', 'Metabolic Syndrome', 'Middle Aged', 'Nutrition Surveys', 'Odds Ratio', 'Olfaction Disorders', 'Prevalence', 'Republic of Korea', 'Severity of Illness Index', 'Smoking', 'Surveys and Questionnaires', 'Tinnitus']
| 30,352,085
|
[['F01.145.317.269'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.418', 'N01.400.500'], ['C18.452.394.968.500.570', 'C18.452.625'], ['M01.060.116.630'], ['E05.318.308.980.485', 'N05.715.360.300.800.469', 'N06.850.505.616', 'N06.850.520.308.980.469'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['C10.597.751.600', 'C23.888.592.763.550'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['Z01.252.474.557.750'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['F01.145.805'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['C09.218.458.670', 'C10.597.751.418.670', 'C23.888.592.763.393.670']]
|
['Psychiatry and Psychology [F]', 'Organisms [B]', 'Health Care [N]', 'Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
The endometrial receptivity array for diagnosis and personalized embryo transfer as a treatment for patients with repeated implantation failure.
|
OBJECTIVE: To demonstrate the clinical value of the endometrial receptivity array (ERA) in patients with repeated implantation failure (RIF), for guiding their personalized embryo transfer (pET) as a novel therapeutic strategy.DESIGN: Prospective interventional multicenter clinical trial.SETTING: University-affiliated infertility and private clinics.PATIENT(S): Eighty-five RIF patients and 25 comparison patients.INTERVENTION(S): Endometrial sampling and pET guided by ERA.MAIN OUTCOME MEASURE(S): A receptive (R) or nonreceptive (NR) endometrial status according to ERA. Pregnancy (PR) and implantation (IR) rates after pET.RESULT(S): The ERA test gave an R result of 74.1% in RIF patients versus 88% in control subjects. Clinical follow-up was possible in 29 RIF patients, in whom pET was performed, resulting in 51.7% PR and 33.9% IR. The IRs and PRs in the 6 months after the biopsy showed that pregnancy was not related to the local injury. Twenty-two RIF patients (25.9%) were NR, and in 15 of them a second ERA validated a displacement of the window of implantation (WOI). In eight of them, pET was performed on the day designated by the ERA, resulting in 50.0% PR and 38.5% IR. These results should be considered as preliminary.CONCLUSION(S): There is an increased percentage of WOI displacement in RIF patients compared with comparison group patients, leading to the concept of pET as a therapeutic strategy. Rescue of NR patients by pET in a displaced WOI results in similar PR and IR.
|
['Abortion, Habitual', 'Adult', 'Biopsy', 'Embryo Implantation', 'Embryo Transfer', 'Endometrium', 'Female', 'Humans', 'Infertility, Female', 'Microarray Analysis', 'Middle Aged', 'Pregnancy', 'Prognosis', 'Treatment Outcome', 'Young Adult']
| 23,756,099
|
[['C13.703.039.089'], ['M01.060.116'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['G08.686.784.170.104.500'], ['E02.875.800.500', 'E05.820.800.500'], ['A05.360.319.679.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C13.351.500.365.700'], ['E05.588.570'], ['M01.060.116.630'], ['G08.686.784.769'], ['E01.789'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Evaluation of the protective efficacy of a commercial vaccine against different antigenic groups of H9N2 influenza viruses in chickens.
|
Despite the long-term vaccination programs implemented in China, H9N2 avian influenza viruses (AIVs) continue to persist in chicken populations, even in vaccinated flocks. We previously demonstrated that H9N2 AIV isolated from chickens in China also underwent antigenic drift and evolved into distinct antigenic groups (C, D and E). To understand whether antigenic drift of viruses away from the vaccine strain partially contributed to the circulation of H9N2 AIV in China, we evaluated the protective efficacy of a commercial vaccine against different antigenic groups of H9N2 AIV. Challenge experiments using vaccinated chickens indicated that the vaccine prevented shedding of antigenic group C viruses, but not those of the more recent groups D and E. Vaccinated chickens, even those with vaccine-induced HI titers of 1:1024, shed virus after being infected with A/chicken/Shandong/ZB/2007, a representative virus of antigenic group D. Genetic analysis showed that the representative viruses of antigenic groups D and E possessed greater numbers of amino acid substitutions in the hemagglutinin protein compared to the vaccine strain and the antigenic group C virus, and many of which were located in antigenic sites. Our results indicated that the persistence of H9N2 AIV in China might be due to incomplete vaccine protection, and that the avian influenza vaccine should be regularly evaluated and updated to maintain optimal protection. Furthermore, the avian influenza vaccination policy also needs to be re-assessed, and increased veterinary biosecurity on farms, rather than vaccine application alone, should be implemented to prevent and control avian influenza.
|
['Animals', 'Antigens, Viral', 'Chickens', 'China', 'Influenza A Virus, H9N2 Subtype', 'Influenza Vaccines', 'Influenza in Birds']
| 22,019,289
|
[['B01.050'], ['D23.050.327'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['Z01.252.474.164'], ['B04.820.480.968.405.400.900'], ['D20.215.894.899.302'], ['C01.925.782.620.300', 'C22.131.450']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Geographicals [Z]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
|
Detection of leachables and cytotoxicity after exposure to methacrylate- and epoxy-based root canal sealers in vitro.
|
Root canal sealing materials may have toxic potential in vitro depending on the cell line, cytotoxicity assay, material chemistry, and degree of polymer curing. The aims of the present study were to detect leaching components from epoxy- or methacrylate-based root canal sealers and to investigate the degree of cytotoxicity after exposure to extracts from these materials. Qualitative determination of substances released from the materials was performed by gas- and liquid chromatography/mass spectrometry. Submandibular salivary gland acinar cell death (apoptosis/necrosis) was determined using a fluorescence staining/microscopy technique. The major leachable monomer from the epoxy-based material was bisphenol-A diglycidyl ether (BADGE), whereas leachables from the methacrylate-based materials were mainly triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA), hydroxyethyl methacrylate (HEMA), and polyethyleneglycol dimethacrylate (PEGDMA). Exposure to diluted extracts of cured methacrylate-based materials caused a postexposure time-dependent increase in cell death. This effect was not demonstrated as a result of exposure to undiluted extract of cured epoxy-based material. Extracts of all fresh materials induced apoptosis significantly, but at lower dilutions of the epoxy- than the methacrylate-based materials. The degree of leaching, determined from the relative chromatogram peak heights of eluates from the methacrylate-based sealer materials, corresponded with the degree of cell death induced by extracts of these materials.
|
['Acinar Cells', 'Animals', 'Cell Culture Techniques', 'Cell Death', 'Chromatography, Liquid', 'Cytotoxins', 'Epoxy Resins', 'Gas Chromatography-Mass Spectrometry', 'Polymethacrylic Acids', 'Rats', 'Root Canal Filling Materials', 'Salivary Glands']
| 24,028,598
|
[['A11.031'], ['B01.050'], ['E01.370.225.500.223', 'E05.200.500.265', 'E05.242.223', 'E05.481.500.249'], ['G04.146'], ['E05.196.181.400'], ['D27.888.569.213'], ['D05.750.716.822.461', 'D25.720.716.822.461', 'J01.637.051.720.716.822.461'], ['E05.196.181.349.500', 'E05.196.566.500'], ['D02.241.081.069.800', 'D05.750.716.822.111.650', 'D25.720.716.822.111.650', 'J01.637.051.720.716.822.111.650'], ['B01.050.150.900.649.313.992.635.505.700'], ['D25.339.859', 'J01.637.051.339.859'], ['A03.556.500.760', 'A10.336.779', 'A14.549.760']]
|
['Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Autologous adipose-derived stem cell transplantation enhances healing of wound with exposed bone in a rat model.
|
OBJECTIVES: Soft tissue wounds with exposed bone often require extended healing times and can be associated with severe complications. We describe the ability of artificial dermis with autogenic adipose-derived stem cells (ADSCs) to promote the healing of wounds with exposed bone in a rat model.METHODS: Adipose tissues harvested from the bilateral inguinal regions of Wistar rats were used as ADSCs. Rats were randomly divided into control and ADSC groups to investigate the efficacy of ADSC transplantation for wound healing (n = 20 per group). Soft tissue defects were created on the heads of the rats and were covered with artificial dermis with or without the seeded ADSCs. Specimens from these rats were evaluated using digital image analysis, histology, immunohistochemistry, cell labeling, and real-time reverse-transcription polymerase chain reaction (real-time RT-PCR).RESULTS: The average global wound area was significantly smaller in the ADSC group than in the control group on days 3, 7, and 14 after surgery (p<0.05). After 14 days, the blood vessel density in the wound increased by 1.6-fold in the ADSC group compared with that in the control group (p<0.01). Real-time RT-PCR results showed higher Fgfb and Vegf expression levels at all time points, and higher Tgfb1 and Tgfb3 expression levels until 14 days after surgery in the ADSC group than in the control group (p<0.05).CONCLUSIONS: In wounds with exposed bone, autogenic ADSCs can promote vascularization and wound healing. Use of this cell source has multiple benefits, including convenient clinical application and lack of ethical concerns.
|
['Adipose Tissue', 'Animals', 'Biomarkers', 'Cell Differentiation', 'Cell Proliferation', 'Disease Models, Animal', 'Female', 'Immunohistochemistry', 'Rats', 'Real-Time Polymerase Chain Reaction', 'Soft Tissue Injuries', 'Stem Cell Transplantation', 'Stem Cells', 'Transplantation, Autologous', 'Wound Healing']
| 31,083,652
|
[['A10.165.114'], ['B01.050'], ['D23.101'], ['G04.152'], ['G04.161.750', 'G07.345.249.410.750'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['B01.050.150.900.649.313.992.635.505.700'], ['E05.393.620.500.706'], ['C26.808'], ['E02.095.147.500.500', 'E04.936.225.687'], ['A11.872'], ['E04.936.664'], ['G16.762.891']]
|
['Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Absence of ribosomal DNA amplification in the meroistic (telotrophic) ovary of the large milkweed bug Oncopeltus fasciatus (Dallas) (Hemiptera: Lygaeidae).
|
In the typical meroistic insect ovary, the oocyte nucleus synthesizes little if any RNA. Nurse cells or trophocytes actively synthesize ribosomes which are transported to and accumulated by the oocyte. In the telotrophic ovary a morphological separation exists, the nurse cells being localized at the apical end of each ovariole and communicating with the ooocytes via nutritive cords. In order to determine whether the genes coding for ribosomal RNA (rRNA) are amplified in the telotrophic ovary of the milkweed bug Oncopeltus fasciatus, the percentages of the genome coding for ribosomal RNA in somatic cells, spermatogenic cells, ovarian follicles, and nurse cells were compared. The oocytes and most of the nurse cells of O. fasciatus are uninucleolate. DNA hybridizing with ribosomal RNA is localized in a satellite DNA, the density of which is 1.712 g/cm(-3). The density of main-band DNA is 1.694 g/cm(-3). The ribosomal DNA satellite accounts for approximately 0.2% of the DNA in somatic and gametogenic tissues of both males and females. RNA-DNA hybridization analysis demonstrates that approximately 0.03% of the DNA in somatic tissues, testis, ovarian follicles, and isolated nurse cells hybridizes with ribosomal RNA. The fact that the percentage of DNA hybridizing with rRNA is the same in somatic and in male and female gametogenic tissues indicates that amplification of ribosomal DNA does not occur in nurse cells and that if it occurs in oocytes, it represents less than a 50-fold increase in ribosomal DNA. An increase in total genome DNA accounted by polyploidization appears to provide for increasing the amount of ribosomal DNA in the nurse cells.
|
['Animals', 'Cell Nucleus', 'Cytosine', 'DNA', 'DNA Replication', 'DNA, Satellite', 'Female', 'Genes', 'Guanine', 'Hemiptera', 'Male', 'Oocytes', 'Ovary', 'RNA, Ribosomal', 'Testis']
| 1,158,969
|
[['B01.050'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['D03.383.742.698.421'], ['D13.444.308'], ['G02.111.225', 'G05.226'], ['D13.444.308.480', 'G02.111.570.080.708.800.150', 'G05.360.080.708.800.150', 'G05.360.340.024.220.150', 'G05.360.340.024.850.150'], ['G05.360.340.024.340'], ['D03.633.100.759.758.399.454'], ['B01.050.500.131.617.412'], ['A05.360.490.690.680', 'A11.497.497.600'], ['A05.360.319.114.630', 'A05.360.576.497', 'A06.300.312.497'], ['D13.444.735.686'], ['A05.360.444.849', 'A05.360.576.782', 'A06.300.312.782']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A case of pediatric cardiomyopathy with severely restrictive physiology.
|
A rare case of a 6-year-old male with idiopathic familial cardiomyopathy manifesting severely restrictive physiology is reported. The patient showed congestive heart failure with dilatation of both atria with a normal ventricular cavity. A square-root configuration was revealed in the ventricular pressure tracings. His elder brother had died of hypertrophic cardiomyopathy at the age of 3 years. Endomyocardial biopsy disclosed marked disorganization of muscle bundles with hypertrophy of the myocytes and interstitial fibrosis. The patient died suddenly during hospitalization. Autopsy revealed diffuse hypertrophy of both the ventricular walls and the ventricular septum with extensive myocardial disorganization and interstitial fibrosis. These advanced myopathic changes in the myocardium may have been related to the restrictive physiology in this case.
|
['Bundle-Branch Block', 'Cardiomyopathy, Hypertrophic', 'Cardiomyopathy, Restrictive', 'Child', 'Diagnosis, Differential', 'Electrocardiography', 'Hemodynamics', 'Humans', 'Male', 'Myocardial Contraction', 'Myocardium']
| 1,487,458
|
[['C14.280.067.558.323', 'C14.280.123.500.323', 'C23.550.073.425.100'], ['C14.280.238.100', 'C14.280.484.048.750.070.160'], ['C14.280.238.160'], ['M01.060.406'], ['E01.171'], ['E01.370.370.380.240', 'E01.370.405.240'], ['G09.330.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G09.330.580', 'G11.427.494.570'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Regulation of casein kinase I epsilon and casein kinase I delta by an in vivo futile phosphorylation cycle.
|
Casein kinase I delta (CKIdelta) and casein kinase I epsilon (CKIepsilon) have been implicated in the response to DNA damage, but the understanding of how these kinases are regulated remains incomplete. In vitro, these kinases rapidly autophosphorylate, predominantly on their carboxyl-terminal extensions, and this autophosphorylation markedly inhibits kinase activity (Cegielska, A., Gietzen, K. F., Rivers, A., and Virshup, D. M. (1998) J. Biol. Chem. 273, 1357-1364). However, we now report that while these kinases are able to autophosphorylate in vivo, they are actively maintained in the dephosphorylated, active state by cellular protein phosphatases. Treatment of cells with the cell-permeable serine/threonine phosphatase inhibitors okadaic acid or calyculin A leads to rapid increases in kinase intramolecular autophosphorylation. Since CKI autophosphorylation decreases kinase activity, this dynamic autophosphorylation/dephosphorylation cycle provides a mechanism for kinase regulation in vivo.
|
['3T3 Cells', 'Adenosine Triphosphate', 'Animals', 'Casein Kinases', 'Cells, Cultured', 'Enzyme Inhibitors', 'Escherichia coli', 'HeLa Cells', 'Homeostasis', 'Humans', 'Isoenzymes', 'Marine Toxins', 'Mice', 'Okadaic Acid', 'Oxazoles', 'Phosphoprotein Phosphatases', 'Phosphorylation', 'Protein Kinases', 'Rats']
| 9,632,646
|
[['A11.251.210.100', 'A11.329.228.100'], ['D03.633.100.759.646.138.236', 'D13.695.667.138.236', 'D13.695.827.068.236'], ['B01.050'], ['D08.811.913.696.620.682.700.140', 'D12.776.476.150'], ['A11.251'], ['D27.505.519.389'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['G07.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D08.811.348', 'D12.776.800.300'], ['D23.946.580'], ['B01.050.150.900.649.313.992.635.505.500'], ['D02.355.291.705', 'D23.946.580.710'], ['D03.383.129.462'], ['D08.811.277.352.650.625'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D08.811.913.696.620.682'], ['B01.050.150.900.649.313.992.635.505.700']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Immunogenetic studies of the platelet-specific antigen P1A1 (Zw(a)).
|
The phenotype frequency of the platelet-specific antigens P1A1/A2 ( = Zw(a,b)) in the German population (N = 711) is 97.75% and 2.25% respectively. The corresponding gene frequencies are P1A1 0.85; P1A2 0.15. The P1A1 determinant is codominantly inherited (19 families; 151 family members). There is a strong gene dosage effect between P1A1 homo and heterozygous individuals. No definite linkage of the locus P1A1 versus the loci HLA-ABC, ABO, or Rh was found.
|
['Antilymphocyte Serum', 'Blood Platelets', 'Chromosome Mapping', 'Female', 'Genetic Linkage', 'Genotype', 'Histocompatibility Testing', 'Humans', 'Isoantigens', 'Male', 'Pedigree', 'Phenotype']
| 7,068,170
|
[['A12.207.152.846.500.203', 'D12.776.124.486.485.114.573.203', 'D12.776.124.790.651.114.573.203', 'D12.776.377.715.548.114.573.203', 'D20.215.401.203'], ['A11.118.188', 'A15.145.229.188'], ['E05.393.183'], ['G05.348'], ['G05.380'], ['E01.370.225.812.385', 'E05.200.812.385', 'E05.478.594.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D23.050.705'], ['E05.393.673'], ['G05.695']]
|
['Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Failure of infused beta-hydroxybutyrate to decrease proteolysis in man.
|
Ketone bodies have been suggested to have a protein-sparing effect, since infusion of Na-beta-hydroxybutyrate in man decreases plasma alanine concentrations and urinary nitrogen (N) excretion. To test this hypothesis, six normal postabsorptive volunteers were infused with Na-beta-hydroxybutyrate for 3 h. Rates of glucose, leucine carbon, and alanine appearance and disappearance from the plasma space were traced with [3-3H]glucose, L-[6,6,6-2H3]leucine, and [2,3,3,3-2H4]alanine. Rates of leucine N appearance and disappearance and the rate of transfer of leucine N to alanine were assessed with [15N]leucine. During ketone body infusion, plasma alanine decreased (P less than 0.05), whereas plasma leucine increased (P less than 0.05). Rates of alanine appearance increased (5.3 +/- 0.3 to 7.8 +/- 0.6 mumol/kg X min), but the increase in its rate of disappearance was slightly greater, accounting for the decrease in plasma alanine concentration. Leucine N flux and the rate and percent of leucine N transferred to alanine increased, whereas leucine carbon flux was unchanged. To determine the effect of the alkalemia induced by Na-beta-hydroxybutyrate, four additional subjects were infused with NaHCO3. Alkalemia had no effect on leucine N or carbon flux or on the rate of appearance of alanine, but increased the rate of alanine disappearance, resulting in a decrease in the plasma alanine concentration. Since the rate of appearance of leucine carbon was unaltered during the infusion of Na-beta-hydroxybutyrate, it is unlikely that hyperketonemia per se decreases proteolysis in postabsorptive man.
|
['3-Hydroxybutyric Acid', 'Adolescent', 'Adult', 'Alanine', 'Animals', 'Bicarbonates', 'Blood Glucose', 'Blood Proteins', 'Dogs', 'Female', 'Glucagon', 'Humans', 'Hydrogen-Ion Concentration', 'Hydroxybutyrates', 'Insulin', 'Ketone Bodies', 'Kinetics', 'Leucine', 'Male', 'Sodium Bicarbonate']
| 6,298,041
|
[['D02.241.081.114.937.349', 'D02.241.511.429.349', 'D02.522.585.087', 'D10.251.400.143.781.500'], ['M01.060.057'], ['M01.060.116'], ['D12.125.042'], ['B01.050'], ['D01.200.275.150.100', 'D01.248.497.158.165.100'], ['D09.947.875.359.448.500'], ['D12.776.124'], ['B01.050.150.900.649.313.750.250.216.200'], ['D06.472.699.587.730.500', 'D12.644.548.586.730.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['D02.241.081.114.937', 'D02.241.511.429', 'D10.251.400.143.781'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D02.522.585'], ['G01.374.661', 'G02.111.490'], ['D12.125.070.637', 'D12.125.142.441'], ['D01.200.275.150.100.800', 'D01.857.625']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Rapid and simple purification of human immunodeficiency virus 1 epitope gp41.
|
In order to develop a reliable and inexpensive serodiagnostic method, part of the transmembrane glycoprotein gene of HIV-1, gp41', (HIV-env 548-646) was cloned into an expression vector, pCT10 with a sequence encoding a hydroxylamine cleavage site and with a part of Lac Z gene (Lac 2": 834 base pairs) as a fusion partner. Overexpression of Lac Z"-gp41' was induced in E. coli and the gp41' fusion protein was purified to homogeneity by centrifugation, hydroxylamine cleavage and an ion-exchange chromatography. Western blot analysis and enzyme-linked immunosorbant assay (ELISA) using the purified gp41 fragment showed high sensitivity and specificity of gp41 as an antigen to detect anti HIV-1 antibodies in testing human sera. These results suggest that this simple and rapid purification method is reliable for obtaining a large quantity of purified gp41'.
|
['Base Sequence', 'Cloning, Molecular', 'DNA, Viral', 'Enzyme-Linked Immunosorbent Assay', 'Epitopes', 'Escherichia coli', 'Genes, env', 'Genetic Vectors', 'HIV Envelope Protein gp41', 'HIV Infections', 'HIV-1', 'Humans', 'Molecular Sequence Data', 'Plasmids', 'Virology']
| 7,679,396
|
[['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['E05.393.220'], ['D13.444.308.568'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['D23.050.550'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['G05.360.340.024.340.364.875.172', 'G05.360.340.358.024.875.172', 'G05.360.340.358.840.500.172'], ['G05.360.337'], ['D12.776.543.512.500.330', 'D12.776.964.775.325.164.200', 'D12.776.964.775.562.500.200', 'D12.776.964.970.880.325.164.200', 'D12.776.964.970.880.910.330', 'D23.050.327.520.330'], ['C01.221.250.875', 'C01.221.812.640.400', 'C01.778.640.400', 'C01.925.782.815.616.400', 'C01.925.813.400', 'C20.673.480'], ['B04.820.650.589.650.350.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453.245.667'], ['G05.360.600'], ['H01.158.273.540.859']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Bi-Force: large-scale bicluster editing and its application to gene expression data biclustering.
|
The explosion of the biological data has dramatically reformed today's biological research. The need to integrate and analyze high-dimensional biological data on a large scale is driving the development of novel bioinformatics approaches. Biclustering, also known as 'simultaneous clustering' or 'co-clustering', has been successfully utilized to discover local patterns in gene expression data and similar biomedical data types. Here, we contribute a new heuristic: 'Bi-Force'. It is based on the weighted bicluster editing model, to perform biclustering on arbitrary sets of biological entities, given any kind of pairwise similarities. We first evaluated the power of Bi-Force to solve dedicated bicluster editing problems by comparing Bi-Force with two existing algorithms in the BiCluE software package. We then followed a biclustering evaluation protocol in a recent review paper from Eren et al. (2013) (A comparative analysis of biclustering algorithms for gene expressiondata. Brief. Bioinform., 14:279-292.) and compared Bi-Force against eight existing tools: FABIA, QUBIC, Cheng and Church, Plaid, BiMax, Spectral, xMOTIFs and ISA. To this end, a suite of synthetic datasets as well as nine large gene expression datasets from Gene Expression Omnibus were analyzed. All resulting biclusters were subsequently investigated by Gene Ontology enrichment analysis to evaluate their biological relevance. The distinct theoretical foundation of Bi-Force (bicluster editing) is more powerful than strict biclustering. We thus outperformed existing tools with Bi-Force at least when following the evaluation protocols from Eren et al. Bi-Force is implemented in Java and integrated into the open source software package of BiCluE. The software as well as all used datasets are publicly available at http://biclue.mpi-inf.mpg.de.
|
['Algorithms', 'Animals', 'Cluster Analysis', 'Computer Simulation', 'Databases, Genetic', 'Gene Expression Profiling', 'Gene Ontology', 'Humans', 'Models, Genetic', 'Oligonucleotide Array Sequence Analysis', 'Principal Component Analysis', 'Software']
| 24,682,815
|
[['G17.035', 'L01.224.050'], ['B01.050'], ['E05.318.740.250', 'N05.715.360.750.200', 'N06.850.520.830.250'], ['L01.224.160'], ['L01.313.500.750.300.188.400.325', 'L01.470.750.750.325'], ['E05.393.332'], ['H01.158.273.343.249.099', 'H01.770.644.145.350.124', 'L01.224.050.375.480.500.500', 'L01.313.500.750.300.550.500.500', 'L01.453.245.945.079.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.395.397'], ['E05.393.661.640', 'E05.393.760.640', 'E05.588.570.660', 'E05.601.640'], ['E05.318.740.562'], ['L01.224.900']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
|
Ultrastructure of Giardia duodenalis trophozoites group from hamster: some relevant aspects.
|
Trophozoites of Giardia duodenalis group obtained from fragments or scratched of hamster's mucosa were examined by transmission electron microscopy. The fine structure of the trophozoites are presented and compared with those described for other animals. Some of the trophozoites present the cytoplasm full of glycogen, rough endoplasmic reticulum-like structures and homogeneous inclusions not enclosed by membranes, recognized as lipid drops, which had not been observed in Giardia from other animals. The adhesive disk is composed of a layer of microtubules, from which fibrous ribbons extend into the cytoplasm; these ribbons are linked by layer of cross-bridge filaments that shows an intermediary dense band, described for the first time in this paper. The authors regard this band as the result of the cross-bridge filaments slinding in the medium region between adjacent fibrous ribbons, and suggest a contractile activity for them. The role of the adhesive disk on the trophozoite mechanism of attachment to host mucosa is also discussed.
|
['Animals', 'Cricetinae', 'Giardia', 'Mesocricetus', 'Microscopy, Electron']
| 1,842,435
|
[['B01.050'], ['B01.050.150.900.649.313.992.635.075.250'], ['B01.237.385'], ['B01.050.150.900.649.313.992.635.075.250.500'], ['E01.370.350.515.402', 'E05.595.402']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Using iron deficiency tests for colorectal cancer screening: a feasibility study in one UK general practice.
|
Iron deficiency is common at presentation in colorectal cancer. Testing for it may complement other screening tests such as faecal occult blood testing and sigmoidoscopy. We therefore examined the feasibility of offering iron deficiency testing to patients in a primary care setting in the UK, offering testing to all 1240 patients aged 55-74 years in one general practice in South Wales, UK. Patients with abnormal results were assessed and offered further investigations. Five hundred and fifty-one people (44.4%) attended for iron deficiency blood tests, of whom 26 patients (4.7%) were iron deficient and offered endoscopic assessment. This identified two cases of benign neoplasia amenable to treatment and no cases of cancer. Iron deficiency testing in a screening context appeared feasible although uptake may be low.
|
['Aged', 'Anemia, Iron-Deficiency', 'Clinical Protocols', 'Colorectal Neoplasms', 'Family Practice', 'Feasibility Studies', 'Humans', 'Middle Aged', 'Wales']
| 15,304,148
|
[['M01.060.116.100'], ['C15.378.071.196.300', 'C18.452.565.100'], ['E02.183', 'N05.715.360.330.125'], ['C04.588.274.476.411.307', 'C06.301.371.411.307', 'C06.405.249.411.307', 'C06.405.469.158.356', 'C06.405.469.491.307', 'C06.405.469.860.180'], ['H02.403.340.500'], ['E05.318.372.550', 'E05.337.675', 'N05.715.360.330.550', 'N06.850.520.450.550'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['Z01.542.363.914']]
|
['Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
|
An equilibrium study of metal ion binding to human plasma coagulation factor XIII.
|
1. The binding of Ca2+ to plasma coagulation Factor XIII from man and from cow caused a small decrease in the intrinsic fluorescence of the protein with a dissociation constant of 0.1 mM. A similar decrease was observed with the thrombin-activated Factors (Factors XIIa). The decrease in protein fluorescence was also caused by both Ni2+ and Mn2+ but not by Mg2+. 2. 45Ca2+ binding was directly demonstrated by equilibrium dialysis. Ca2+ at 0.2 mM bound to Factor XIII (a2b2) and Factor XIIIa (a'2b2) but not to isolated b2-protein. A tight-binding site for Ca2+ is associated with the a-subunits. 3. The Ca2+ essential for the enzyme activity of Factor XIII from man, pig and cow can be replaced by Ni2+, Cu2+, La3+, Mn2+, Fe3+, Y3+, Co2+, Sr2+ or Tb3+, but not by Mg2+.
|
['Animals', 'Binding Sites', 'Calcium', 'Cations, Divalent', 'Cattle', 'Dialysis', 'Factor XIII', 'Humans', 'Kinetics', 'Spectrometry, Fluorescence', 'Thrombin']
| 629,762
|
[['B01.050'], ['G02.111.570.120'], ['D01.268.552.100', 'D01.552.539.288', 'D23.119.100'], ['D01.248.497.300.333'], ['B01.050.150.900.649.313.500.380.271'], ['E05.196.353', 'G02.186'], ['D08.622.505', 'D12.776.124.125.475', 'D12.776.811.243.505', 'D23.119.475'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G01.374.661', 'G02.111.490'], ['E05.196.712.516.600.676', 'E05.196.867.726'], ['D08.811.277.656.300.760.855', 'D08.811.277.656.959.350.855', 'D12.776.124.125.890', 'D23.119.960']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Inhibitory effect of live-attenuated Listeria monocytogenes-based vaccines expressing MIA gene on malignant melanoma.
|
Listeria monocytogenes (LM), a Gram-positive facultative intracellular bacterium, can be used as an effective exogenous antigen expression vector in tumor-target therapy. But for successful clinical application, it is necessary to construct attenuated LM stain that is safe yet retains the potency of LM based on the full virulent pathogen. In this study, attenuated LM and recombinants of LM expressing melanoma inhibitory activity (MIA) were constructed successfully. The median lethal dose (LD(50)) and invasion efficiency of attenuated LM strains were detected. The recombinants were utilized for immunotherapy of animal model of B16F10 melanoma. The level of MIA mRNA expression in tumor tissue was detected by using real-time polymerase chain reaction (PCR) with specific sequence, meanwhile the anti-tumor immune response was assayed by flow cytometric analysis and enzyme-linked immunosorbent spot (ELISPOT) assay. The results showed the toxicity and invasiveness of attenuated LM were decreased as compared with LM, and attenuated LM expressing MIA, especially the double-genes attenuated LM recombinant, could significantly induce anti-tumor immune response and inhibit tumor growth. This study implicates attenuated LM may be a safer and more effective vector for immunotherapy of melanoma.
|
['Animals', 'Cancer Vaccines', 'Extracellular Matrix Proteins', 'Listeria monocytogenes', 'Male', 'Melanoma', 'Mice', 'Mice, Inbred C57BL', 'Vaccines, Attenuated']
| 22,886,976
|
[['B01.050'], ['D20.215.894.200'], ['D12.776.860.300'], ['B03.353.500.500.500', 'B03.510.100.500.500', 'B03.510.460.400.410.485.500'], ['C04.557.465.625.650.510', 'C04.557.580.625.650.510', 'C04.557.665.510'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['D20.215.894.811']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Universal diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses].
|
The diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses (IAVs) was developed. We proposed a simple method of the fluorescent labeling of genomic segments of all known IAVs subtypes, the composition of the hybridization buffer, as well as the software of the data processing. 48 IAVs strains of different subtypes were analyzed using our microarray. All of them were identified, while 45 of 48 strains were unambiguously subtyped.
|
['Animals', 'Genome, Viral', 'Humans', 'Influenza A virus', 'Influenza, Human', 'Lab-On-A-Chip Devices', 'Molecular Typing', 'Oligonucleotide Array Sequence Analysis', 'Orthomyxoviridae Infections', 'RNA, Viral', 'Software']
| 24,640,169
|
[['B01.050'], ['G05.360.340.358.840'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B04.820.480.968.405.400'], ['C01.748.310', 'C01.925.782.620.365', 'C08.730.310'], ['E07.305.343.500'], ['E01.370.225.875.150.125.457', 'E05.200.875.150.125.457', 'E05.393.542'], ['E05.393.661.640', 'E05.393.760.640', 'E05.588.570.660', 'E05.601.640'], ['C01.925.782.620'], ['D13.444.735.828'], ['L01.224.900']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Information Science [L]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
The diagnostic properties of laboratory tests for rabies.
|
We have prospectively followed 915 victims bitten by 664 dogs between January 1984 and December 1985 to assess death and severe neurological disability. These dogs were brought either by the owner or bite victims to Ta Pra Diagnostic Centre for detection of rabies. Each patient was followed every three months by mail for at least one year after the bite. In addition, intensive follow-up in the community was undertaken on 235 patients who failed to respond to mail follow-up, all 23 who reported events as well as 523 of the 720 mail-responders. Hospital records were examined for all patients requiring treatment. All patients with non-fatal events were also examined at the university hospital. Blind independent comparison of the three diagnostic tests (Sellers stain, fluorescent antibody test and mouse inoculation test) for the presence or absence of rabies in animal brains were performed to assess the accuracy of local routine diagnostic test methods. In case of disagreement between test methods, the result on mouse inoculation was considered definitive for the presence or absence of rabies. Test results were mailed to all victims. Seven hundred and twenty out of 955 subjects responded to the mail follow-up giving a response rate of 75.4%. Intensive follow-ups were successful in all subjects who responded. Of 235 subjects who did not respond to the mail follow-up, only 195 (83%) were located. The remaining 40 bite victims who did not respond to mail follow-up and could not be located were all bitten by animals who did not have rabies.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Animals', 'Clinical Laboratory Techniques', 'Dogs', 'Fluorescent Antibody Technique', 'Follow-Up Studies', 'Humans', 'Predictive Value of Tests', 'Rabies', 'Rabies Vaccines', 'Vaccination']
| 3,326,844
|
[['B01.050'], ['E01.370.225', 'E05.200'], ['B01.050.150.900.649.313.750.250.216.200'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['C01.925.782.580.830.750'], ['D20.215.894.899.700'], ['E02.095.465.425.400.530.890', 'E05.478.550.600.890', 'N02.421.726.758.310.890', 'N06.850.780.200.425.900', 'N06.850.780.680.310.890']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
The beta(2)-adrenergic receptor delivers an antiapoptotic signal to cardiac myocytes through G(i)-dependent coupling to phosphatidylinositol 3'-kinase.
|
Recent studies have shown that chronic beta-adrenergic receptor (beta-AR) stimulation alters cardiac myocyte survival in a receptor subtype-specific manner. We examined the effect of selective beta(1)- and beta(2)-AR subtype stimulation on apoptosis induced by hypoxia or H(2)O(2) in rat neonatal cardiac myocytes. Although neither beta(1)- nor beta(2)-AR stimulation had any significant effect on the basal level of apoptosis, selective beta(2)-AR stimulation protected myocytes from apoptosis. beta(2)-AR stimulation markedly increased mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) activation as well as phosphatidylinositol-3'-kinase (PI-3K) activity and Akt/protein kinase B phosphorylation. beta(1)-AR stimulation also markedly increased MAPK/ERK activation but only minimally activated PI-3K and Akt. Pretreatment with pertussis toxin blocked beta(2)-AR-mediated protection from apoptosis as well as the beta(2)-AR-stimulated changes in MAPK/ERK, PI-3K, and Akt/protein kinase B. The selective PI-3K inhibitor, LY 294002, also blocked beta(2)-AR-mediated protection, whereas inhibition of MAPK/ERK activation at an inhibitor concentration that blocked agonist-induced activation but not the basal level of activation had no effect on beta(2)-AR-mediated protection. These findings demonstrate that beta(2)-ARs activate a PI-3K-dependent, pertussis toxin-sensitive signaling pathway in cardiac myocytes that is required for protection from apoptosis-inducing stimuli often associated with ischemic stress.
|
['Apoptosis', 'Cells, Cultured', 'Chromones', 'Enzyme Inhibitors', 'Flavonoids', 'GTP-Binding Protein alpha Subunits, Gi-Go', 'Humans', 'Hydrogen Peroxide', 'Mitogen-Activated Protein Kinases', 'Morpholines', 'Myocardium', 'Pertussis Toxin', 'Phosphatidylinositol 3-Kinases', 'Receptors, Adrenergic, beta-1', 'Receptors, Adrenergic, beta-2', 'Signal Transduction', 'Virulence Factors, Bordetella']
| 11,110,775
|
[['G04.146.954.035'], ['A11.251'], ['D03.383.663.283.266', 'D03.633.100.150.266'], ['D27.505.519.389'], ['D03.383.663.283.266.450', 'D03.633.100.150.266.450'], ['D08.811.277.040.330.300.200.100.200', 'D12.644.360.360.100.200', 'D12.776.157.325.332.100.200', 'D12.776.476.375.100.200', 'D12.776.543.325.100.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D01.248.497.158.685.750.424', 'D01.339.431.374.424', 'D01.650.550.750.400', 'D02.389.338.253'], ['D08.811.913.696.620.682.700.567', 'D12.644.360.450', 'D12.776.476.450'], ['D03.383.533.640'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['D08.811.913.400.725.115.680', 'D23.946.123.946.690', 'D23.946.896.980.690'], ['D08.811.913.696.620.500'], ['D12.776.543.750.670.300.300.340.100', 'D12.776.543.750.695.150.300.340.700', 'D12.776.543.750.720.330.300.340.100'], ['D12.776.543.750.670.300.300.340.200', 'D12.776.543.750.695.150.300.340.725', 'D12.776.543.750.720.330.300.340.200'], ['G02.111.820', 'G04.835'], ['D23.946.123.946', 'D23.946.896.980']]
|
['Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Injuries and use of protective equipment among college in-line skaters.
|
In-line skating injuries and protective gear use were explored in a sample of college students (n = 217). A minority of respondents wore protective gear. One third of skaters had experienced at least one minor injury, and a smaller percentage had experienced fractures or head injuries. Most minor injuries occurred during the first 1-2 times skating, while more serious injuries tended to occur after at least 50 times on in-line skates. Psychosocial predictors of protective gear use were explored. Four major Health Belief Model constructs (perceived barriers to wearing gear, perceived susceptibility to injury, perceived severity of injury, and perceived benefits of wearing gear) were significant predictors of protective gear use. The Health Belief Model, tested using regression and structural equation modelling, predicted gear typically worn, frequency of gear use, and injuries received while in-line skating. Implications for increasing protective gear use are described.
|
['Adolescent', 'Adult', 'Athletic Injuries', 'Female', 'Health Knowledge, Attitudes, Practice', 'Humans', 'Male', 'Protective Devices', 'Skating', 'Students']
| 9,006,646
|
[['M01.060.057'], ['M01.060.116'], ['C26.115'], ['F01.100.150.500', 'N05.300.150.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E07.700', 'J01.637.708'], ['I03.450.642.845.700'], ['M01.848']]
|
['Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
|
Cloning and expression of cDNA for arginine-specific ADP-ribosyltransferase from chicken bone marrow cells.
|
Two arginine-specific ADP-ribosyltransferase cDNAs (designated AT1 and AT2) were cloned from chicken bone marrow cells. Each cDNA encodes a different peptide of 312 amino acid residues. Homology of deduced amino acid sequences between AT1 and AT2 was 78.3%. We found all six combined peptide sequences of 222 amino acid residues derived from purified chicken heterophil ADP-ribosyltransferase (Mishima, K., Terashima, M., Obara, S., Yamada, K., Imai, K., and Shimoyama, M. (1991) J. Biochem. (Tokyo) 110, 388-394) in the deduced amino acid sequence of AT1, with two amino acid mismatches. Arginine-specific ADP-ribosyltransferase activity was detected in culture medium of COS 7 cells transiently transfected with AT1 cDNA, while activity from the cells transfected with AT2 cDNA was found in both culture medium and cell lysate. AT1 transferase required 2-mercaptoethanol for the activity. The activity was inhibited in the presence of NaCl while AT2 enzyme was activated by either agent. On zymographic in situ gel analysis, estimated molecular masses of the AT1, AT2 and purified chicken heterophil transferases were 32, 34, and 27.5 kDa, respectively. Northern blot analysis with specific probes to AT1 or AT2 cDNAs revealed about a 1.5-kilobase message in chicken bone marrow cells but no signals were observed in heterophils, spleen, and liver of chicken or human HL-60 cells. Highly conserved regions were observed among the deduced amino acid sequences of AT1, AT2, rabbit skeletal muscle transferase, and rodent T-cell surface antigen RT6s.
|
['ADP Ribose Transferases', 'Animals', 'Antigens, Differentiation, T-Lymphocyte', 'Arginine', 'Base Sequence', 'Bone Marrow', 'Chickens', 'Cloning, Molecular', 'DNA Primers', 'DNA, Complementary', 'GPI-Linked Proteins', 'Gene Expression', 'Histocompatibility Antigens', 'Membrane Glycoproteins', 'Molecular Sequence Data', 'Poly(ADP-ribose) Polymerases', 'RNA, Messenger', 'Rats', 'Recombinant Proteins', 'Sequence Alignment', 'Sequence Homology, Amino Acid']
| 7,961,658
|
[['D08.811.913.400.725.115'], ['B01.050'], ['D23.050.301.264.894', 'D23.101.100.894'], ['D12.125.068.050', 'D12.125.095.104', 'D12.125.142.087'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['A15.382.216'], ['B01.050.150.900.248.350.150', 'B01.050.150.900.248.690.192'], ['E05.393.220'], ['D13.695.578.424.450.275', 'D27.720.470.530.600.223.600'], ['D13.444.308.497.220', 'D13.444.600.223.500', 'D27.720.470.530.600.223.260'], ['D12.776.395.550.448', 'D12.776.543.484.500', 'D12.776.543.550.418'], ['G05.297'], ['D23.050.301.500', 'D23.050.705.552'], ['D12.776.395.550', 'D12.776.543.550'], ['L01.453.245.667'], ['D08.811.913.400.725.115.690'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.828'], ['E05.393.751'], ['G02.111.810.200', 'G05.810.200']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Hemodynamic effects of a new right ventricular assist device.
|
A right ventricular assist device (VAD) based on the principle of counterpulsation has been developed at our institution. The device is a valveless, pneumatically actuated, 40 cc, sac-type pump, with a single inlet-outlet port. For right ventricular support, the "Uniport" pump is anastamosed end-to-side to the pulmonary artery. In previous experimental trials, the device has been shown to impart minimal trauma to blood components. In this study, biventricular failure was induced in eight Holstein calves by normothermic ischemia during cardiopulmonary bypass. A Pierce-Donachy left VAD (LVAD) was used for left ventricular support following the ischemic insult. Hemodynamic measurements were obtained throughout the study, and each animal served as its own control. A significant increase in post injury cardiac output (33.5 +/- 11.4%) was obtained with use of the Uniport and LVAD, as compared to use of the LVAD alone (p less than or equal to 0.005). Other hemodynamic parameters of right heart failure, including right atrial pressure (RAP), pulmonary artery pressure (PAP), and left atrial pressure (LAP) were not significantly affected. These data suggest that the Uniport right ventricular assist device significantly improves cardiac output in this model of moderate right ventricular failure. Additional studies are required, however, to optimize pump stroke volume, and to further define the performance envelope of the device.
|
['Animals', 'Cardiac Output', 'Cattle', 'Equipment Design', 'Heart Failure', 'Heart-Assist Devices', 'Hemodynamics', 'Male', 'Ventricular Function, Right']
| 2,252,737
|
[['B01.050'], ['E01.370.370.380.150', 'G09.330.380.124'], ['B01.050.150.900.649.313.500.380.271'], ['E05.320'], ['C14.280.434'], ['E04.050.430', 'E07.695.300.300', 'E07.858.082.374.300'], ['G09.330.380'], ['G09.330.955.900']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Retinoic acid regulates insulin-like growth factor II expression in a neuroblastoma cell line.
|
Insulin-like growth factors (IGF-I and IGF-II) are mitogenic polypeptides that play an important role in normal growth and development. IGF-II has been shown to stimulate the growth of neuroblastoma tumors in an autocrine and paracrine fashion. Critical in determining the role of IGF-II in tumorigenesis is the necessity to delineate factors affecting the transcription of IGF-II in normal and tumor tissues. To date such factors are poorly characterized. In this study we find that retinoic acid (RA), a naturally occurring morphogen, that has been shown to be indispensable in the development of the chick limb bud, stimulates an increase in IGF-II messenger RNA (mRNA) in the Lan-1-15N neuroblastoma cell line. This increase in IGF-II is coincident with RA mediated inhibition of DNA synthesis. An increase in the steady state levels of IGF-II mRNA is detectable within 2 h of RA treatment and maximal by 24 h. In RA-treated Lan-1-15N cells, IGF-II mRNA levels are regulated at the level of new gene transcription and result in an increase in IGF-II protein in the culture supernatant. These studies suggest one mechanism affecting the production of IGF-II in vivo may be mediated by RA and detail a model system by which transcriptional regulation of IGF-II mRNA can be analyzed.
|
['Cell Division', 'Cell Line', 'Cell Nucleus', 'DNA Replication', 'Gene Expression Regulation, Neoplastic', 'Humans', 'Insulin-Like Growth Factor II', 'Kinetics', 'Neuroblastoma', 'Poly A', 'RNA', 'RNA, Messenger', 'Thymidine', 'Transcription, Genetic', 'Tretinoin']
| 1,375,906
|
[['G04.144.220', 'G04.161.750.500', 'G05.113', 'G07.345.249.410.750.500'], ['A11.251.210'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['G02.111.225', 'G05.226'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.937.420', 'D12.776.124.862.425', 'D12.776.467.937.420', 'D23.529.937.420'], ['G01.374.661', 'G02.111.490'], ['C04.557.465.625.600.590.650.550', 'C04.557.470.670.590.650.550', 'C04.557.580.625.600.590.650.550'], ['D13.695.578.550.500'], ['D13.444.735'], ['D13.444.735.544'], ['D03.383.742.680.705', 'D13.570.230.855', 'D13.570.685.705'], ['G02.111.873', 'G05.297.700'], ['D02.455.326.271.665.202.495.818.500', 'D02.455.426.392.368.367.379.249.700.860.500', 'D02.455.849.131.495.818.800', 'D02.455.849.291.925.500', 'D23.767.261.700.780']]
|
['Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Acetate-bridged platinum(III) complexes derived from cisplatin.
|
Oxidation of the acetate-bridged half-lantern platinum(II) complex cis-[Pt(II)(NH(3))(2)(ì-OAc)(2)Pt(II)(NH(3))(2)](NO(3))(2), [1](NO(3))(2), with iodobenzene dichloride or bromine generates the halide-capped platinum(III) species cis-[XPt(III)(NH(3))(2)(ì-OAc)(2)Pt(III)(NH(3))(2)X](NO(3))(2), where X is Cl in [2](NO(3))(2) or Br in [3](NO(3))(2), respectively. These three complexes, characterized structurally by X-ray crystallography, feature short (?2.6 ?) Pt-Pt separations, consistent with formation of a formal metal-metal bond upon oxidation. Elongated axial Pt-X distances occur, reflecting the strong trans influence of the metal-metal bond. The three structures are compared to those of other known dinuclear platinum complexes. A combination of (1)H, (13)C, (14)N, and (195)Pt NMR spectroscopy was used to characterize [1](2+)-[3](2+) in solution. All resonances shift downfield upon oxidation of [1](2+) to [2](2+) and [3](2+). For the platinum(III) complexes, the (14)N and (195)Pt resonances exhibit decreased line widths by comparison to those of [1](2+). Density functional theory calculations suggest that the decrease in the (14)N line width arises from a diminished electric field gradient at the (14)N nuclei in the higher valent compounds. The oxidation of [1](NO(3))(2) with the alternative oxidizing agent bis(trifluoroacetoxy)iodobenzene affords the novel tetranuclear complex cis-[(O(2)CCF(3))Pt(III)(NH(3))(2)(ì-OAc)(2)Pt(III)(NH(3))(ì-NH(2))](2)(NO(3))(4), [4](NO(3))(4), also characterized structurally by X-ray crystallography. In solution, this complex exists as a mixture of species, the identities of which are proposed.
|
['Acetates', 'Cisplatin', 'Crystallography, X-Ray', 'Magnetic Resonance Spectroscopy', 'Models, Molecular', 'Molecular Conformation', 'Organoplatinum Compounds', 'Oxidation-Reduction']
| 22,946,515
|
[['D02.241.081.018', 'D10.251.400.045'], ['D01.210.375', 'D01.625.125', 'D01.710.100'], ['E05.196.309.742.225'], ['E05.196.867.519'], ['E05.599.595'], ['G02.111.570.820'], ['D02.691.788'], ['G02.700', 'G03.295.531']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Accurate relief of the die surface of the wax pattern prior to casting.
|
A quick, simple technique has been described to mark, identify, and remove interferences from the inner surface of a wax pattern. Problems in the seating of simple and complex castings are virtually eliminated.
|
['Dental Casting Technique', 'Dental Models', 'Gold Alloys', 'Technology, Dental', 'Waxes']
| 325,202
|
[['E06.780.250', 'E06.912.115'], ['E06.261', 'J01.897.280.500.545.129.200', 'L01.178.820.090.545.129.200'], ['D01.379.375', 'D01.552.033.533', 'D25.058.451', 'D25.339.208.534', 'J01.637.051.058.451', 'J01.637.051.339.208.534'], ['E06.912', 'H02.010.800', 'J01.897.724'], ['D10.945']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
|
Thermal sensitivity and protein anti-adsorption of hydroxypropyl cellulose-g- poly(2-(methacryloyloxy) ethyl phosphorylcholine).
|
Zwitterionic graft copolymers, hydroxypropyl cellulose graft poly(2-(methacryloyloxy) ethyl phosphorylcholine) (HPC-g-PMPC) with well-defined architecture were synthesized by atom transfer radical polymerization (ATRP). The self-assembly behaviors and thermal sensitivity of HPC-g-PMPC copolymers and their correlations with graft density and side chain length were investigated in details. HPC-g-PMPC copolymers can self-assemble into spherical aggregate structure above the critical aggregation concentration (CAC) at room temperature. Meanwhile, the size of the aggregates mainly depended on the graft density. The obtained aggregates were thermal sensitive and their low critical solution temperature (LCST) was efficiently regulated by varying the graft density. Above the LCST, the aggregates were transferred into aggregates with core-shell structure, in which the HPC rich core was stabilized by the PMPC rich shell. The interaction between the HPC-g-PMPC aggregates and BSA was investigated. The results indicated that the anti-adsorption of BSA on the aggregates surface depended on the length and graft density of the PMPC zwitterionic side chains.
|
['Adsorption', 'Cellulose', 'Methacrylates', 'Phosphorylcholine', 'Polymers', 'Temperature']
| 27,987,988
|
[['G01.030', 'G02.020'], ['D05.750.078.562.180', 'D09.698.365.180', 'D25.720.099.500', 'J01.637.051.720.099.500'], ['D02.241.081.069.600'], ['D02.092.877.883.333.700', 'D02.675.276.232.700'], ['D05.750', 'D25.720', 'J01.637.051.720'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Cancer Internet search activity on a major search engine, United States 2001-2003.
|
BACKGROUND: To locate online health information, Internet users typically use a search engine, such as Yahoo! or Google. We studied Yahoo! search activity related to the 23 most common cancers in the United States.OBJECTIVE: The objective was to test three potential correlates of Yahoo! cancer search activity--estimated cancer incidence, estimated cancer mortality, and the volume of cancer news coverage--and to study the periodicity of and peaks in Yahoo! cancer search activity.METHODS: Yahoo! cancer search activity was obtained from a proprietary database called the Yahoo! Buzz Index. The American Cancer Society's estimates of cancer incidence and mortality were used. News reports associated with specific cancer types were identified using the LexisNexis "US News" database, which includes more than 400 national and regional newspapers and a variety of newswire services.RESULTS: The Yahoo! search activity associated with specific cancers correlated with their estimated incidence (Spearman rank correlation, rho = 0.50, P = .015), estimated mortality (rho = 0.66, P = .001), and volume of related news coverage (rho = 0.88, P < .001). Yahoo! cancer search activity tended to be higher on weekdays and during national cancer awareness months but lower during summer months; cancer news coverage also tended to follow these trends. Sharp increases in Yahoo! search activity scores from one day to the next appeared to be associated with increases in relevant news coverage.CONCLUSIONS: Media coverage appears to play a powerful role in prompting online searches for cancer information. Internet search activity offers an innovative tool for passive surveillance of health information-seeking behavior.
|
['Cost of Illness', 'Health Education', 'Humans', 'Incidence', 'Internet', 'Neoplasms', 'Research', 'Survival Analysis', 'United States']
| 15,998,627
|
[['N03.219.151.165', 'N05.715.360.300.800.438.375.182', 'N06.850.520.308.980.438.475.046'], ['I02.233.332', 'N02.421.726.407'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['L01.224.230.110.500'], ['C04'], ['H01.770.644'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998'], ['Z01.107.567.875']]
|
['Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 1
|
The efficacy and persistent anthelmintic effect of ivermectin in sheep.
|
The direct efficacy and the long-term persistent anthelmintic effect of an oral suspension and an injectable formulation of ivermectin at a dose rate of 0.2 mg kg-1 was studied in sheep. Lambs were infected experimentally with Haemonchus contortus, Trichostrongylus vitrinus and Cooperia curticei. After 3 weeks they were treated with an oral suspension or with an injection, and reinfected with the same dose of larvae 3, 6 or 10 days after treatment. Post-mortem worm counts showed no persistent effect of the oral suspension. The injectable formulation showed an excellent persistent effect for up to 10 days against H. contortus. Reinfection with C. curticei 3 days after treatment resulted in a 64% reduction of the worm burden, but reinfection after 6 and 10 days was 100% reduced. The reduction of T. vitrinus was 46% after 3 days and 92% after 6 and 10 days.
|
['Administration, Oral', 'Animals', 'Feces', 'Haemonchiasis', 'Injections, Subcutaneous', 'Intestinal Diseases, Parasitic', 'Ivermectin', 'Parasite Egg Count', 'Sheep', 'Sheep Diseases', 'Trichostrongyloidiasis', 'Trichostrongylosis']
| 8,291,186
|
[['E02.319.267.100'], ['B01.050'], ['A12.459'], ['C01.610.335.508.700.775.825.400'], ['E02.319.267.530.620'], ['C01.610.432', 'C06.405.469.452'], ['D02.540.576.500.997'], ['E01.370.225.932.600', 'E05.200.932.600'], ['B01.050.150.900.649.313.500.380.791'], ['C22.836'], ['C01.610.335.508.700.775.825'], ['C01.610.335.508.700.775.825.842']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Outcomes of childhood conduct problem trajectories in early adulthood: findings from the ALSPAC study.
|
Although conduct problems in childhood are stably associated with problem outcomes, not every child who presents with conduct problems is at risk. This study extends previous studies by testing whether childhood conduct problem trajectories are predictive of a wide range of other health and behavior problems in early adulthood using a general population sample. Based on 7,218 individuals from the Avon longitudinal study of parents and children, a three-step approach was used to model childhood conduct problem development and identify differences in early adult health and behavior problems. Childhood conduct problems were assessed on six occasions between age 4 and 13 and health and behavior outcomes were measured at age 18. Individuals who displayed early-onset persistent conduct problems throughout childhood were at greater risk for almost all forms of later problems. Individuals on the adolescent-onset conduct problem path consumed more tobacco and illegal drugs and engaged more often in risky sexual behavior than individuals without childhood conduct problems. Levels of health and behavior problems for individuals on the childhood-limited path were in between those for stable low and stable high trajectories. Childhood conduct problems are pervasive and substantially affect adjustment in early adulthood both in at-risk samples as shown in previous studies, but also in a general population sample. Knowing a child's developmental course can help to evaluate the risk for later maladjustment and be indicative of the need for early intervention.
|
['Adolescent', 'Age Factors', 'Child', 'Child Development', 'Child, Preschool', 'Conduct Disorder', 'Early Intervention, Educational', 'Female', 'Humans', 'Longitudinal Studies', 'Male', 'Risk Factors', 'Social Adjustment']
| 24,197,169
|
[['M01.060.057'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.406'], ['F01.525.200', 'G07.345.374.750'], ['M01.060.406.448'], ['F03.625.094.300'], ['N02.421.143.130.320', 'N02.421.726.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['F01.145.813.621']]
|
['Named Groups [M]', 'Health Care [N]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Efficacy and safety of loxapine for inhalation in the treatment of agitation in patients with schizophrenia: a randomized, double-blind, placebo-controlled trial.
|
OBJECTIVE: The objective of this study was to assess the efficacy and safety of inhaled loxapine in the treatment of agitation in patients with psychotic disorders.METHOD: In this randomized, double-blind, placebo-controlled study, 129 agitated patients with schizophrenia or schizoaffective disorder (DSM-IV criteria) were randomized to receive in a clinical or hospital setting a single inhalation of 5 or 10 mg of loxapine or placebo administered using the Staccato loxapine for inhalation device. The inhalation device delivered thermally generated drug aerosol to the deep lung for rapid absorption. The primary efficacy measure was change on the Positive and Negative Syndrome Scale-excited component (PANSS-EC) 2 hours following treatment. Secondary outcomes included the Clinical Global Impressions-Improvement scale (CGI-I), Behavioral Activity Rating Scale (BARS), and time to first rescue medication. The study was conducted between September 2006 and January 2007.RESULTS: Differences were statistically significant (P < .05) between placebo and both 5-mg and 10-mg doses on the CGI-I and the CGI-I responder analyses at 2 hours and in time to first rescue medication, and they were statistically significant (P < .05) between placebo and 10-mg loxapine on the PANSS-EC 20 minutes after administration continuing through 2 hours and in change from baseline BARS. Three serious adverse events occurred at least 6 days after treatment, but none were judged related to study treatment. The most common adverse events were sedation and dysgeusia (22% and 17%, respectively, in the 10-mg group, and 14% and 9%, respectively, in the placebo group).CONCLUSIONS: Inhaled loxapine was generally safe and well tolerated and produced rapid improvement in agitated patients with psychotic disorders. Statistically significant differences in efficacy were found for the 10-mg dose compared with placebo, with results suggesting 5 mg may be effective. The delivery of loxapine by inhalation may provide a rapid, well-tolerated option for treating acute psychotic agitation that allows patients to avoid the aversive effects and loss of autonomy often associated with use of intramuscular medications. Further investigation of this new loxapine formulation is warranted.TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT00369577.
|
['Administration, Inhalation', 'Adult', 'Aerosols', 'Antipsychotic Agents', 'Double-Blind Method', 'Drug Administration Schedule', 'Female', 'Humans', 'Loxapine', 'Male', 'Middle Aged', 'Psychomotor Agitation', 'Psychotic Disorders', 'Treatment Outcome', 'Young Adult']
| 21,294,997
|
[['E02.319.267.050'], ['M01.060.116'], ['D20.280.055', 'D26.255.165.055'], ['D27.505.696.277.950.040', 'D27.505.954.427.210.950.040', 'D27.505.954.427.700.872.331'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['E02.319.283'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D03.633.300.347.500'], ['M01.060.116.630'], ['C10.597.350.600', 'C10.597.606.881.700', 'C23.888.592.350.600', 'C23.888.592.604.882.700', 'F01.700.875.700'], ['F03.700.675'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['M01.060.116.815']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Organisms [B]', 'Diseases [C]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Attachment of bacterial cells to carbon electrodes.
|
Anodic stripping method was applied to analyze the process of bacterial attachment to the surface of carbon-paste electrodes (CPE). The electrode was immersed for various times in a bacterial cell suspension to allow the cells to attach to its surface. The number of bacterial cells attached to the electrode surface increased along with time. On the other hand, the current derived from the oxidation of a dye, Hoechst, which was adsorbed to the surface after attaching the bacterial cells, decreased along with time. It was considered that the current output, correlated with the amount of dye, adsorbed onto regions where no bacterial cell attached. These results indicate that the bacterial-attachment process can be analyzed by measuring the electric current derived from the dye instead of counting the number of attached cells.
|
['Bacterial Adhesion', 'Carbon', 'Coloring Agents', 'Electrodes', 'Oxidation-Reduction']
| 10,790,776
|
[['G06.099.050'], ['D01.268.150'], ['D27.720.233'], ['E07.305.250'], ['G02.700', 'G03.295.531']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Synthesis and biological activity of unusual nucleosides having 3,4-bis(hydroxymethyl) thietane ring as a sugar moiety.
|
The enantiomerically pure synthesis of 9-[(2'S, 3'S)-bis(hydroxymethyl)thietan-1'-yl]adenine 2,3'-thio analog of oxetanocin A, was achieved via coupling of silylated 6-chloropurine and sulfoxide 16 under Pummerer reaction conditions.
|
['Adenine', 'Antiviral Agents', 'Indicators and Reagents', 'Molecular Structure']
| 10,780,351
|
[['D03.633.100.759.138'], ['D27.505.954.122.388'], ['D27.720.470.410'], ['G02.111.570', 'G02.466']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Agenesis of the corpus callosum: magnetic resonance imaging.
|
Three patients who had complete agenesis and two patients who had partial agenesis of the corpus callosum (ACC) underwent magnetic resonance (MR) imaging. In addition to excellent visualization of the indirect signs of ACC, direct vivid display (short T1) of the corpus callosum on sagittal images allowed better evaluation of subtle abnormalities than has been possible with other modalities. Associated abnormalities were also well-displayed. MR is the initial procedure of choice in evaluation of the corpus callosum.
|
['Agenesis of Corpus Callosum', 'Humans', 'Magnetic Resonance Spectroscopy']
| 3,983,387
|
[['C10.500.034', 'C16.131.666.034', 'C23.300.008'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.196.867.519']]
|
['Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Impact of the transition to ICD-10 on Medicare inpatient hospital payments.
|
OBJECTIVE: On October 1, 2013, the reporting of diagnoses and procedures in the U.S. will transition from the clinical modification of the ninth revision of the International Classification of Diseases (ICD-9-CM) to the tenth revision (ICD-10). We estimate the impact of conversion to ICD-10 on Medicare MS-DRG payments to hospitals using 2009 Medicare data.METHODS: Using the ICD-9-CM MS-DRG v27 (FY 2010), the converted ICD-10 MS-DRG v27, and the ICD-10 to ICD-9-CM Reimbursement Map for fiscal year 2010, we estimate the impact on aggregate payments to hospitals and the distribution of payments across hospitals.RESULTS: Although the transition from the ICD-9-CM to the ICD-10 version of MS-DRGs resulted in 1.68 percent of the patients being assigned to a different MS-DRG, payment increases and decreases due to the changes in MS-DRG assignment essentially netted out, resulting in a minimal impact on aggregate payments to hospitals (+0.05 percent) and on the distribution of payments across hospital types (-0.01 to +0.18 percent). Mapping ICD-10 data back to ICD-9-CM, and using the ICD-9-CM MS-DRGs, resulted in 3.66 percent of patients being assigned to a different MS-DRG, a modest decrease in aggregate payments to hospitals (-0.34 percent), and modest changes in the distribution of payments across hospital types (-0.14 to -0.46 percent).DISCUSSION: As demonstrated by MS-DRGs, a direct conversion of an application to ICD-10 can produce consistent results with the ICD-9-CM version of the application. However, the use of mappings between ICD-10 and ICD-9-CM will produce less consistent results, especially if the mapping is not tailored to the specific application.
|
['Diagnosis-Related Groups', 'Economics, Hospital', 'Humans', 'Inpatients', 'International Classification of Diseases', 'Medicare', 'Reimbursement Mechanisms', 'United States']
| 22,340,773
|
[['N03.219.521.710.305.200.080'], ['N03.219.262'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.643.470'], ['L01.453.245.945.400'], ['N03.219.521.346.506.564.663', 'N03.219.521.576.343.840', 'N03.706.615.696'], ['N03.219.521.710.305'], ['Z01.107.567.875']]
|
['Health Care [N]', 'Organisms [B]', 'Named Groups [M]', 'Information Science [L]', 'Geographicals [Z]']
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 1
|
Anatomopathological findings in hangings: a retrospective autopsy study.
|
BACKGROUND: In this retrospective autopsy study, we aimed to review the anatomopathological findings observed in cases of hanging death for a five year period and to evaluate the role of contributing factors such as age, sex, type of hanging and localization of the ligature knot.METHODS: Autopsy reports of 102 hanging cases performed by the Department of Forensic Medicine, Faculty of Medicine of Pamukkale University, between January 2007 and September 2011, were retrospectively reviewed.RESULTS: In the 102 hanging cases 73 of the victims were males (71.6%) and 29 (28.4%) were females, with a mean age of 40.97 ± 17.41 years. All cases were suicidal hanging. Fifty four cases (52.9%) were typical hanging, with the ligature knot located posteriorly. There were petechial hemorrhage on the face and eye lids in 46 (45.1%), ecchymoses of the cervicale muscles in 43 (42.2%), and fractures of the neck structure(s) in 69 cases (67.6%).CONCLUSIONS: The incidence of neck structure fractures increased with age. In addition, there was no correlation between the incidence of neck structure fractures and sex or type of hanging.
|
['Adolescent', 'Adult', 'Age Distribution', 'Aged', 'Aged, 80 and over', 'Asphyxia', 'Ecchymosis', 'Female', 'Forensic Pathology', 'Humans', 'Male', 'Middle Aged', 'Neck Injuries', 'Purpura', 'Retrospective Studies', 'Sex Distribution', 'Suicide', 'Turkey', 'Young Adult']
| 23,275,431
|
[['M01.060.057'], ['M01.060.116'], ['I01.240.050', 'N01.224.033', 'N06.850.505.400.050'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C23.550.260.095', 'C26.103'], ['C15.378.100.452', 'C23.550.414.625', 'C23.888.885.312'], ['H02.403.330.300', 'H02.403.650.249', 'I01.198.780.937.460'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C26.700'], ['C15.378.100.802', 'C23.550.414.950', 'C23.888.885.687'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['F01.145.126.980.875', 'I01.880.735.856'], ['Z01.252.245.500.850'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
Prophylaxis of neutropenic fever with ciprofloxacin in patients with acute myeloid leukemia treated with intensive chemotherapy.
|
AIMS: Neutropenic fever is one of the most serious complications after induction chemotherapy in acute myeloid leukemia (AML). Prophylaxis with antibiotics for prevention of neutropenic fever in AML is controversial and there are few studies on this issue from developing countries.METHODS: In this retrospective study, we analyzed the clinical data and outcome of patients with AML who did or did not receive prophylactic ciprofloxacin 500 mg BD for neutropenic fever.RESULTS: A total of 69 AML patients were treated by "3 + 7" protocol for their first induction chemotherapy. Prophylaxis was given to 25 of them. Incidence of neutropenic fever was the same in both groups (80% vs 82%). Duration of fever and the mortality rate were also similar in both groups.CONCLUSION: It seems that in developing countries, using prophylactic ciprofloxacin has no significant effect on the incidence of neutropenic fever and the outcome of the AML patients.
|
['Adolescent', 'Aged', 'Antibiotic Prophylaxis', 'Ciprofloxacin', 'Female', 'Fever', 'Humans', 'Incidence', 'Induction Chemotherapy', 'Leukemia, Myeloid, Acute', 'Male', 'Middle Aged', 'Neutropenia', 'Retrospective Studies']
| 24,330,539
|
[['M01.060.057'], ['M01.060.116.100'], ['E02.319.162.150', 'E02.319.703.150'], ['D03.633.100.810.835.322.186'], ['C23.888.119.344'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E02.319.499', 'E02.860.500'], ['C04.557.337.539.275'], ['M01.060.116.630'], ['C15.378.553.546.184.564'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses.
|
Alphaviruses cause serious diseases that pose a potential health threat to both humans and livestock. The nonstructural protein 2 (nsp2) encoded by alphaviruses is a multifunctional enzyme that is essential for viral replication and maturation. Its 39-kDa C-terminal domain (nsp2pro) is a cysteine protease that is responsible for cleaving a viral polyprotein at three sites to generate nonstructural proteins 1, 2, 3 and 4. In the present study, we evaluated nsp2pro domains from the following three sources as reagents for site-specific cleavage of fusion proteins: Venezuelan Equine Encephalitis Virus (VEEV), Semliki Forest Virus (SFV) and Sindbis Virus (SIN). All three alphavirus proteases cleaved model fusion protein substrates with high specificity but they were much less efficient enzymes than potyviral proteases from tobacco etch virus (TEV) and tobacco vein mottling virus (TVMV). Oligopeptide substrates were also cleaved with very low efficiency by the alphavirus proteases. We conclude that, in general, alphavirus nsp2pro proteases are not very useful tools for the removal of affinity tags from recombinant proteins although they do remain promising therapeutic targets for the treatment of a variety of diseases.
|
['Alphavirus', 'Cloning, Molecular', 'Cysteine Endopeptidases', 'Dithiothreitol', 'Edetic Acid', 'Glycerol', 'Humans', 'Hydrogen-Ion Concentration', 'Mercaptoethanol', 'Molecular Weight', 'Phosphines', 'Protein Structure, Tertiary', 'Recombinant Fusion Proteins', 'Sodium Chloride', 'Substrate Specificity', 'Temperature']
| 19,013,248
|
[['B04.820.578.875.054'], ['E05.393.220'], ['D08.811.277.656.262.500', 'D08.811.277.656.300.200'], ['D02.033.800.196', 'D02.886.740.224', 'D09.853.196'], ['D02.092.782.258.368.250', 'D02.241.081.018.253'], ['D02.033.800.875.500', 'D09.853.875.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.300'], ['D02.033.375.534', 'D02.886.489.409'], ['G02.494'], ['D01.695.525', 'D02.705.621'], ['G02.111.570.820.709.610'], ['D12.776.828.300'], ['D01.210.450.150.875', 'D01.857.650'], ['G02.111.835'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Hip fracture rates in Hong Kong and the United States, 1988 through 1989.
|
OBJECTIVES: Prior studies have suggested that hip fracture rates are substantially lower in Asian countries than in the United States. However, comparisons have been limited by unavailability of recent data, differences in case definition, lack of data from similar time periods, and small sample sizes. This study sought to examine trends by age and sex, with separate statistics for those aged 85 or older.METHODS: Hospital discharge data were used to obtain hip fracture incidence in Hong Kong and the United States from 1988 through 1989.RESULTS: Within each population, women had higher hip fracture rates than men. Fracture rates in the United States were significantly higher for both sexes than rates in Hong Kong. For persons over the age of 80, rates of hip fracture among White US males exceeded those for Hong Kong women. Inclusion of transferred cases in hip fracture rates minimized differences between the countries.CONCLUSIONS: Despite increasing hip fracture rates in Hong Kong, those rates are still substantially lower than the rates in the United States. Identifying factors responsible for this variation may prove useful in the search for preventive strategies.
|
['Aged', 'Aged, 80 and over', 'Female', 'Hip Fractures', 'Hong Kong', 'Hospital Records', 'Humans', 'Male', 'Middle Aged', 'Sex Ratio', 'United States']
| 8,484,451
|
[['M01.060.116.100'], ['M01.060.116.100.080'], ['C26.404.061.425', 'C26.531.750', 'C26.558.276.425'], ['Z01.252.474.164.450'], ['E05.318.308.940.425', 'L01.399.250.900.425', 'N04.452.859.380', 'N05.715.360.300.715.380', 'N06.850.520.308.940.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['G05.815', 'I01.240.800.815', 'N01.224.803.815', 'N06.850.505.400.850.815'], ['Z01.107.567.875']]
|
['Named Groups [M]', 'Diseases [C]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]', 'Health Care [N]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 1
| 1
| 1
| 1
|
Non-ionic surfactants as novel intranasal absorption enhancers: in vitro and in vivo characterization.
|
OBJECTIVE: To explore the potential of non-ionic surfactants as novel intranasal absorption enhancers.METHODS: Taking sumatriptan succinate (SMS) as a model drug, influence of different non-ionic surfactants, including laurate sucrose ester (SE), cremophor EL and poloxamer 188, on the intranasal absorption of SMS was investigated using an in situ nasal perfusion technique in rats. Ciliotoxicity of the non-ionic surfactants was evaluated using an in situ toad palate model. In vivo behavior of the selected formulations was studied in rats.RESULTS: All the non-ionic surfactants investigated increased the intranasal absorption of SMS remarkably but with varied extent and trend. Moreover, it was revealed that at the same concentration, laurate SE had better permeation-enhancing effect than that of cremophor EL and poloxamer 188. The ciliotoxicity results showed that all the non-ionic surfactants were regarded as safe at selected concentrations. Based on the in situ absorption data and ciliotoxicity results, the following three samples, 0.5% laurate SE, 0.1% cremophor EL and 0.5% poloxamer 188 were selected for in vivo absorption studies in rats. Among them, 0.5% laurate SE group presented the highest enhancing effect, followed by 0.1% cremophor EL and 0.5% poloxamer 188 group, with absolute bioavailability 29.99%, 22.64% and 20.90%, respectively.CONCLUSIONS: Laurate SE is a promising intranasal absorption enhancer.
|
['Administration, Intranasal', 'Animals', 'Biological Availability', 'Chemistry, Pharmaceutical', 'Glycerol', 'Laurates', 'Male', 'Nasal Absorption', 'Nasal Mucosa', 'Poloxamer', 'Rats', 'Rats, Sprague-Dawley', 'Sumatriptan', 'Surface-Active Agents']
| 25,347,689
|
[['E02.319.267.120.655.500'], ['B01.050'], ['G03.787.151', 'G07.690.725.129'], ['H01.158.703.007', 'H01.181.466'], ['D02.033.800.875.500', 'D09.853.875.500'], ['D10.251.500.410'], ['G03.015.500.703.500', 'G03.787.024.500.703.500', 'G07.690.725.015.500.703.500', 'G09.772.813.500'], ['A04.531.520', 'A04.760.600', 'A10.615.550.760.600'], ['D02.033.455.250.700.682', 'D05.750.741.667', 'D25.720.741.667', 'J01.637.051.720.741.667'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D02.065.884.750', 'D02.886.590.700.750', 'D03.633.100.473.914.907'], ['D27.720.877']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
NOV (CCN3) regulation in the growth plate and CCN family member expression in cartilage neoplasia.
|
Growth plate chondrocytes undergo a coordinated differentiation process resulting in terminal differentiation and new bone formation. Enchondromas are pre-malignant, benign cartilaginous lesions that arise from growth plate chondrocytes that fail to undergo terminal differentiation. NOV (nephroblastoma overexpressed) is a member of the CCN family of proteins, which share a common multi-modular organization. While the role of NOV in chondrocyte development and cartilage neoplasia is not known, other CCN family members play a role in chondrocyte differentiation, or are differentially regulated in cartilage neoplasia. In embryonic murine growth plates, NOV was expressed in pre-hypertrophic and early hypertrophic chondrocytes. PTHrP treatment (which inhibits terminal differentiation) decreased NOV expression in murine femurs maintained in organ culture, and decreased the activity of a NOV reporter construct in vitro. Expression of the CCN family members NOV, CTGF, CYR61, and WISP-1 was examined in 15 chondrosarcomas of various grades and in three enchondromas. Expression of all of the family members was lower in the higher-grade tumours. As identification of the grade of cartilage neoplasia can sometimes be difficult using histology alone, the level of expression of CCN family members could be a useful adjunct in the determination of tumour grade.
|
['Animals', 'Bone Neoplasms', 'CCN Intercellular Signaling Proteins', 'Cartilage Diseases', 'Chondrocytes', 'Chondroma', 'Chondrosarcoma', 'Connective Tissue Growth Factor', 'Culture Media', 'Cysteine-Rich Protein 61', 'Femur', 'Gene Expression Regulation, Neoplastic', 'Growth Plate', 'Humans', 'Hypertrophy', 'Immediate-Early Proteins', 'Immunohistochemistry', 'Intercellular Signaling Peptides and Proteins', 'Intracellular Signaling Peptides and Proteins', 'Mice', 'Mice, Inbred Strains', 'Mitogens', 'Nephroblastoma Overexpressed Protein', 'Oncogene Proteins', 'Organ Culture Techniques', 'Parathyroid Hormone-Related Protein', 'Polymerase Chain Reaction', 'Promoter Regions, Genetic', 'Proto-Oncogene Proteins']
| 14,648,665
|
[['B01.050'], ['C04.588.149', 'C05.116.231'], ['D12.644.276.200', 'D12.776.467.200', 'D12.776.860.300.200', 'D23.529.237'], ['C05.182', 'C17.300.182'], ['A11.329.171'], ['C04.557.450.565.265'], ['C04.557.450.565.280', 'C04.557.450.795.300'], ['D12.644.276.200.100', 'D12.776.467.200.100', 'D12.776.860.300.200.100', 'D23.529.237.100'], ['D27.720.470.305', 'E07.206'], ['D12.644.276.200.200', 'D12.776.467.200.200', 'D12.776.860.300.200.200', 'D23.529.237.200'], ['A02.835.232.043.150'], ['G05.308.370'], ['A02.835.232.251.352'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.300.775'], ['D12.776.460', 'D12.776.964.925.968'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D12.644.276', 'D12.776.467', 'D23.529'], ['D12.644.360', 'D12.776.476'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520', 'B01.050.150.900.649.313.992.635.505.500.400'], ['D27.505.519.593.624'], ['D12.644.276.200.500', 'D12.776.467.200.500', 'D12.776.860.300.200.500', 'D23.101.140.450', 'D23.529.237.600'], ['D12.776.624.664'], ['E05.481.500.484'], ['D06.472.699.591', 'D12.644.276.908', 'D12.644.548.588', 'D12.776.467.890', 'D23.529.890'], ['E05.393.620.500'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['D12.776.624.664.700']]
|
['Organisms [B]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Structural characteristics and bioactive properties of a novel polysaccharide from Flammulina velutipes.
|
A new water-soluble polysaccharide (FVP1) was extracted from Flammulina velutipes by traditional method "water extraction and alcohol precipitation" and purified by column chromatography. Physicochemical characterization showed that FVP1 was a homogeneous polysaccharide with a relative molecular weight of 54.78 kDa. It is composed of mannose (7.74%), glucose (70.41%), and galactose (16.38%). FVP1 (1000 mg/mL) possessed significant immune activity by increasing the secretion of nitric oxide (NO), tumour necrosis factor-á (TNF-á) (3183 ± 133.84 pg/mL), interleukin (IL)-6 (1133.21 ± 39.05 pg/mL), and IL-12 (579.96 ± 74.53 pg/mL) in macrophages. Furthermore, FVP1 showed significant hepatitis B surface antibody (anti-HBV) activity through reducing the expression of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA replication. These results suggest a novel role for FVP1 to be applied as an immunomodulators in dietary supplements to prevent HBV infection.
|
['Animals', 'Cell Survival', 'Cells, Cultured', 'DNA, Viral', 'Dose-Response Relationship, Drug', 'Flammulina', 'Hep G2 Cells', 'Hepatitis B Surface Antigens', 'Hepatitis B e Antigens', 'Hepatitis B virus', 'Humans', 'Mice', 'Microbial Sensitivity Tests', 'Molecular Weight', 'Polysaccharides', 'RAW 264.7 Cells', 'Structure-Activity Relationship', 'Virus Replication']
| 30,007,599
|
[['B01.050'], ['G04.346'], ['A11.251'], ['D13.444.308.568'], ['G07.690.773.875', 'G07.690.936.500'], ['B01.300.179.100.320'], ['A11.251.860.180.432', 'A11.436.348.500'], ['D23.050.327.495.500.475'], ['D23.050.327.495.500.469'], ['B04.280.375.650.425', 'B04.450.390.650.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['G02.494'], ['D09.698'], ['A11.251.210.172.875', 'A11.733.397.815'], ['G02.111.830', 'G07.690.773.997'], ['G06.920.925']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Pituitary and adrenal control of pancreatic endocrine function in the duck.
|
Recent clinical and immunological data suggest that the classical concept of "idiopathic autoimmune diseases" is to be revised. In a normal population, autoimmunity reactions against thyroid gland, gastric mucosa and adrenals develop slowly with increasing age and are found more frequently in women than in men (at least so far as thyroid antibodies are concerned) as is lowering of the functional activity of T lymphocytes. Diabetes takes its place among a series of factors diminishing immunocyte reactivity, and thus enhancing the development of the autoimmunity process. This may perhaps be promoted in some way by genetic factors, perhaps those which also play a definite though as yet ill-defined role in determining the emergence of diabetes. For the present, diabetes mellitus itself must only rarely be considered a consequence of an autoimmune process and then only in certain insulin-dependent cases. By contrast, diabetes appears frequently to be an activator of autoimmune phenomena against tissues other than pancreas, namely thyroid gland, adrenals and gastric mucosa. Awareness of these associations should encourage physicians to seek latent humoral or cellular evidence of autoimmune phenomena in diabetics; this would favour the early recognition of clinically important abnormalities which may accompany the diabetes.
|
['Animals', 'Blood Glucose', 'Corticosterone', 'Ducks', 'Fasting', 'Glucagon', 'Glucose', 'Growth Hormone', 'Hypophysectomy', 'Islets of Langerhans', 'Male', 'Pituitary Gland', 'Pituitary Gland, Anterior', 'Pituitary-Adrenal System']
| 789,141
|
[['B01.050'], ['D09.947.875.359.448.500'], ['D04.210.500.745.745.654.237', 'D06.472.040.585.353.237'], ['B01.050.150.900.248.050.200', 'B01.050.150.900.248.690.345'], ['F01.145.407.400', 'G07.203.650.240.587', 'G07.203.650.353.400'], ['D06.472.699.587.730.500', 'D12.644.548.586.730.500'], ['D09.947.875.359.448'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['E04.270.532', 'E04.525.400'], ['A03.734.414', 'A06.300.414'], ['A06.300.747', 'A06.688.357.750', 'A08.186.211.180.497.352.435.500', 'A08.186.211.200.317.357.352.435.500', 'A08.713.357.750'], ['A06.300.747.500', 'A06.688.357.750.500', 'A08.186.211.180.497.352.435.500.500', 'A08.186.211.200.317.357.352.435.500.500', 'A08.713.357.750.500'], ['A06.300.691']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Ultrasound assessment of endometrial cavity in perimenopausal women on oral progesterone for abnormal uterine bleeding: comparison of diagnostic accuracy of imaging with hysteroscopy-guided biopsy.
|
AIM: To investigate the effect of oral progesterone on the accuracy of imaging studies performed to detect endometrial pathology in comparison to hysteroscopy-guided biopsy in perimenopausal women on progesterone treatment for abnormal uterine bleeding.METHODS: The study population comprised of women aged 40-55 years with complaints of abnormal uterine bleeding who were also undergoing oral progesterone therapy. Women with a uterus ? 12 weeks' gestation size, previous abnormal endometrial biopsy, cervical lesion on speculum examination, abnormal Pap smear, active pelvic infection, adnexal mass on clinical examination or during ultrasound scan and a positive pregnancy test were excluded. A transvaginal ultrasound followed by saline infusion sonography were done. On the following day, a hysteroscopy followed by a guided biopsy of the endometrium or any endometrial lesion was performed. Comparison between the results of the imaging study with the hysteroscopy and guided biopsy was done.RESULTS: The final analysis included 83 patients. For detection of overall pathology, polyp and fibroid transvaginal ultrasound had a positive likelihood ratio of 1.65, 5.45 and 5.4, respectively, and a negative likelihood ratio of 0.47, 0.6 and 0.43, respectively. For detection of overall pathology, polyp and fibroid saline infusion sonography had a positive likelihood ratio of 4.4, 5.35 and 11.8, respectively, and a negative likelihood ratio of 0.3, 0.2 and 0.15, respectively.CONCLUSION: In perimenopausal women on oral progesterone therapy for abnormal uterine bleeding, imaging studies cannot be considered as an accurate method for diagnosing endometrial pathology when compared to hysteroscopy and guided biopsy.
|
['Adult', 'Biopsy', 'Endometrium', 'Female', 'Humans', 'Hysteroscopy', 'Middle Aged', 'Perimenopause', 'Progesterone', 'Progestins', 'Sensitivity and Specificity', 'Ultrasonography', 'Uterine Hemorrhage']
| 21,733,032
|
[['M01.060.116'], ['E01.370.225.500.384.100', 'E01.370.225.998.054', 'E01.370.388.100', 'E04.074', 'E05.200.500.384.100', 'E05.200.998.054', 'E05.242.384.100'], ['A05.360.319.679.490'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.378.330', 'E01.370.388.250.360', 'E04.502.250.360', 'E04.520.360', 'E04.950.300.539'], ['M01.060.116.630'], ['G08.686.157.500.562', 'G08.686.841.249.500.562'], ['D04.210.500.745.745.654.829', 'D06.472.334.734.623', 'D06.472.334.851.687.750'], ['D27.505.696.399.472.858'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['E01.370.350.850'], ['C13.351.500.852.691', 'C23.550.414.993']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
The first cohort of Iranian patients with hyper immunoglobulin E syndrome: A long-term follow-up and genetic analysis.
|
BACKGROUND: Hyper-IgE syndromes (HIES) are distinct diseases characterized by recurrent cutaneous and lung infections, eczema, and elevated serum IgE level.METHODS: In this study, clinical manifestations, immunologic findings, and genetic studies of all patients with HIES in the Iranian national registry database were evaluated.RESULTS: A total of 129 HIES patients with a median age of 14.0 (9.0-24.0) years were followed up for a total of 307.8 patient-years. Genetic studies showed heterozygous STAT3 mutations in 19 patients and homozygous DOCK8 mutation in 16 patients. The mean of National Institutes of Health score in STAT3-deficient patients was higher than in patients with DOCK8 mutation (P = 0.001). It was shown that the presence of pneumatocele and hematologic complication were significantly frequent in STAT3-deficient cases compared to patients with DOCK8 deficiency (P = 0.001 and P = 0.002, respectively). Moreover, the median IgE serum levels were higher in patients with STAT3 gene mutation than in patients with DOCK8 gene mutation (P = 0.02). The eosinophils' count was enhanced in patients with DOCK8 deficiency than in patients with STAT3 gene defects (P = 0.02).CONCLUSION: Specific molecular study of STAT3 and DOCK8 mutations in patients with HIES clinical phenotype could help the physician to definitively characterize the disease. Since HIES showed the highest rate of unsolved combined immunodeficiency, investigation of other genetic and environmental factors could also help in understanding the mechanism of remaining patients as well as providing strategy into therapeutic modalities.
|
['Adolescent', 'Adult', 'Child', 'Cohort Studies', 'Female', 'Follow-Up Studies', 'Guanine Nucleotide Exchange Factors', 'Humans', 'Immunoglobulin E', 'Infections', 'Iran', 'Job Syndrome', 'Lung', 'Male', 'Mutation', 'STAT3 Transcription Factor', 'Skin', 'Young Adult']
| 30,801,830
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.406'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['D12.644.360.325.300', 'D12.776.476.325.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.124.486.485.114.619.312', 'D12.776.124.790.651.114.619.312', 'D12.776.377.715.548.114.619.312'], ['C01'], ['Z01.252.245.500.350'], ['C15.378.553.774.600', 'C16.320.798.688', 'C20.673.774.600', 'C20.673.795.688'], ['A04.411'], ['G05.365.590'], ['D12.644.360.024.342.300', 'D12.776.157.057.186.300', 'D12.776.476.024.430.300', 'D12.776.930.840.300'], ['A17.815'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Diseases [C]', 'Geographicals [Z]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Reproduction and hatchling performance in freshwater turtles associated with a remediated coal fly-ash spill.
|
In 2008 an impoundment retaining wall failed at the Tennessee Valley Authority's coal burning plant in Kingston, Tennessee, releasing large quantities of coal-fly ash into the Emory River. Following extensive remediation of the spill, we captured (in 2011 and 2012) gravid turtles of multiple species in three rivers (two impacted and one reference) within the vicinity of the spill to determine whether there was evidence of the spill influencing reproduction. There was little evidence that river of origin affected reproductive output, hatching success, hatchling size, or hatchling locomotor performance. Although hatching success and hatchling righting ability of pond sliders, Trachemys scripta, was higher in our reference river than in the Emory or Clinch River, respectively, these differences could not be attributed to differences in individual element concentrations in turtle tissues and effect sizes were relatively small. For example, hatching success was reduced by 11% in the spill zone compared to the reference river, an effect that is unlikely substantial enough to influence local population dynamics in light of turtle life history. Our results suggest that residual contamination that remains in the Emory-Clinch system after its remediation poses low risk of excessive element exposure and limited adverse reproductive effects to freshwater turtles. Future monitoring could reveal whether the observed reduction in hatching success gradually attenuates with time, or whether any long-term effects of chronic exposure to low-level contamination emerge over time.
|
['Animals', 'Coal Ash', 'Environmental Exposure', 'Environmental Monitoring', 'Female', 'Male', 'Mass Spectrometry', 'Reproduction', 'Rivers', 'Species Specificity', 'Tennessee', 'Turtles', 'Water Pollutants, Chemical']
| 25,682,257
|
[['B01.050'], ['D20.633.110'], ['N06.850.460.350'], ['N06.850.460.350.080', 'N06.850.780.375'], ['E05.196.566'], ['G08.686.784'], ['G01.311.750', 'G16.500.275.280.650', 'N06.230.232.650'], ['G16.824'], ['Z01.107.567.875.075.775'], ['B01.050.150.900.833.848'], ['D27.888.284.903.655']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Geographicals [Z]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
|
Theflavins and theasinensin A derived from fermented tea have antihyperglycemic and hypotriacylglycerolemic effects in KK-A(y) mice and Sprague-Dawley rats.
|
Although tea polyphenols are reported to improve serum glucose and lipid levels by inhibiting amylase activity and reducing lipid absorption, in vivo data are lacking. We evaluated in vivo the antihyperglycemic and hypotriacylglycerolemic effects of theaflavins (TFs) and theasinensin A (TSA) refined from fermented tea to purities of 12 and 59%, respectively. Feeding male KK-A(y) mice diets with 0.1% TFs or TSA for 6 weeks reduced serum glucose levels by >30% compared to a control diet. Rats fed diets containing 0.2% TFs or TSA for 4 weeks had higher fecal fat excretion and 33% lower hepatic triacylglycerol; hepatic fatty acid synthase activity was not affected. Oral administration of TFs or TSA reduced the increase in serum triacylglycerol after an oral bolus of a fat emulsion. These results indicate TFs and TSA induce antihyperglycemic responses in diabetic mice and are hypotriacylglycerolemic in rats by suppressing intestinal fat absorption.
|
['Animals', 'Benzopyrans', 'Biflavonoids', 'Camellia sinensis', 'Catechin', 'Diabetes Mellitus, Type 2', 'Dietary Supplements', 'Eriobotrya', 'Fermentation', 'Gallic Acid', 'Hypertriglyceridemia', 'Hypoglycemic Agents', 'Hypolipidemic Agents', 'Japan', 'Male', 'Mice', 'Mice, Inbred Strains', 'Phenols', 'Plant Leaves', 'Rats', 'Rats, Sprague-Dawley', 'Tea', 'Triglycerides']
| 24,011,231
|
[['B01.050'], ['D03.383.663.283', 'D03.633.100.150'], ['D03.383.663.283.266.450.190', 'D03.633.100.150.266.450.190'], ['B01.650.940.800.575.912.250.341.998.500.500'], ['D03.383.663.283.240.190', 'D03.383.663.283.266.450.206', 'D03.633.100.150.240.190', 'D03.633.100.150.266.450.206'], ['C18.452.394.750.149', 'C19.246.300'], ['G07.203.300.456', 'J02.500.456'], ['B01.650.940.800.575.912.250.859.937.500.222'], ['G02.111.158.249', 'G03.191.249'], ['D02.241.223.100.300.200', 'D02.241.511.390.200', 'D02.455.426.559.389.127.281.200', 'D02.455.426.559.389.657.410.200'], ['C18.452.584.500.500.851'], ['D27.505.696.422'], ['D27.505.519.186.071', 'D27.505.954.557.500'], ['Z01.252.474.463', 'Z01.639.595'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520', 'B01.050.150.900.649.313.992.635.505.500.400'], ['D02.455.426.559.389.657'], ['A18.024.812'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.750'], ['D20.215.784.844', 'G07.203.100.831', 'J02.200.831'], ['D10.351.801']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]', 'Geographicals [Z]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
|
Lipid specificity of the membrane binding domain of coagulation factor X.
|
Essentials Membrane-binding GLA domains of coagulation factors are essential for proper clot formation. Factor X (FX) is specific to phosphatidylserine (PS) lipids through unknown atomic-level interactions. Molecular dynamics simulations were used to develop the first membrane-bound model of FX-GLA. PS binding modes of FX-GLA were described, and potential PS-specific binding sites identified.SUMMARY: Background Factor X (FX) binds to cell membranes in a highly phospholipid-dependent manner and, in complex with tissue factor and factor VIIa (FVIIa), initiates the clotting cascade. Experimental information concerning the membrane-bound structure of FX with atomic resolution has remained elusive because of the fluid nature of cellular membranes. FX is known to bind preferentially to phosphatidylserine (PS). Objectives To develop the first membrane-bound model of the FX-GLA domain to PS at atomic level, and to identify PS-specific binding sites of the FX-GLA domain. Methods Molecular dynamics (MD) simulations were performed to develop an atomic-level model for the FX-GLA domain bound to PS bilayers. We utilized a membrane representation with enhanced lipid mobility, termed the highly mobile membrane mimetic (HMMM), permitting spontaneous membrane binding and insertion by FX-GLA in multiple 100-ns simulations. In 14 independent simulations, FX-GLA bound spontaneously to the membrane. The resulting membrane-bound models were converted from HMMM to conventional membrane and simulated for an additional 100 ns. Results The final membrane-bound FX-GLA model allowed for detailed characterization of the orientation, insertion depth and lipid interactions of the domain, providing insight into the molecular basis of its PS specificity. All binding simulations converged to the same configuration despite differing initial orientations. Conclusions Analysis of interactions between residues in FX-GLA and lipid-charged groups allowed for potential PS-specific binding sites to be identified. This new structural and dynamic information provides an additional step towards a full understanding of the role of atomic-level lipid-protein interactions in regulating the critical and complex clotting cascade.
|
['1-Carboxyglutamic Acid', 'Animals', 'Binding Sites', 'Cattle', 'Cell Membrane', 'Factor X', 'Kinetics', 'Molecular Docking Simulation', 'Phosphatidylserines', 'Protein Binding', 'Protein Interaction Domains and Motifs', 'Structure-Activity Relationship']
| 28,782,177
|
[['D02.241.081.901.400', 'D12.125.067.625.174', 'D12.125.119.409.174'], ['B01.050'], ['G02.111.570.120'], ['B01.050.150.900.649.313.500.380.271'], ['A11.284.149'], ['D08.622.471', 'D12.776.124.125.400', 'D12.776.811.243.471', 'D23.119.400'], ['G01.374.661', 'G02.111.490'], ['E05.599.595.249', 'L01.224.160.249'], ['D10.570.755.375.760.400.971'], ['G02.111.679', 'G03.808'], ['G02.111.570.820.709.275.750.500'], ['G02.111.830', 'G07.690.773.997']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Information Science [L]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Ventilator-associated pneumonia in patients assisted by veno-arterial extracorporeal membrane oxygenation support: Epidemiology and risk factors of treatment failure.
|
INTRODUCTION: Ventilator-associated pneumonia (VAP) is frequent in Intensive Care Unit (ICU) patients. In the specific case of patients treated with Veno-Arterial Extracorporeal Membrane Oxygenation Support (VA-ECMO), VAP treatment failures (VAP-TF) have been incompletely investigated.METHODS: To investigate the risk factors of treatment failure (VAP-TF) in a large cohort of ICU patients treated with VA-ECMO, we conducted a retrospective study in a Surgical ICU about patients assisted with VA-ECMO between January 1, 2013, and December 31, 2014. Diagnosis of VAP was confirmed by a positive quantitative culture of a respiratory sample. VAP-TF was defined as composite of death attributable to pneumonia and relapse within 28 days of the first episode.RESULTS: In total, 152 patients underwent ECMO support for > 48h. During the VA-ECMO support, 85 (55.9%) patients developed a VAP, for a rate of 60.6 per 1000 ECMO days. The main pathogens identified were Pseudomonas aeruginosa and Enterobacteriaceae. VAP-TF occurred in 37.2% of patients and was associated with an increased 28-day mortality (Hazard Ratio 3.05 [1.66; 5.63], P<0.001), and VA-ECMO assistance duration (HR 1.47 [1.05-2.05], P = 0.025). Risk factors for VAP-TF were renal replacement therapy (HR 13.05 [1.73; 98.56], P = 0.013) and documentation of Pseudomonas aeruginosa (HR 2.36 [1.04; 5.35], P = 0.04).CONCLUSIONS: VAP in patients treated with VA-ECMO is associated with an increased morbidity and mortality. RRT and infection by Pseudomonas aeruginosa appear as strong risks factors of treatment failure. Further studies seem necessary to precise the best antibiotic management in these patients.
|
['Adult', 'Aged', 'Comorbidity', 'Extracorporeal Membrane Oxygenation', 'Female', 'Humans', 'Male', 'Middle Aged', 'Pneumonia, Ventilator-Associated', 'Risk Factors', 'Treatment Failure', 'Treatment Outcome']
| 29,652,913
|
[['M01.060.116'], ['M01.060.116.100'], ['N05.715.350.225', 'N06.850.490.687'], ['E02.880.301', 'E04.292.451'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C01.248.250.500', 'C01.748.610.300.500', 'C08.381.677.300.500', 'C08.730.610.300.500', 'C23.550.291.875.500.500.500'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['E01.789.800.760', 'N04.761.559.590.800.760', 'N05.715.360.575.575.800.760'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Named Groups [M]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
On possible existence of pseudobinary mixed valence fluorides of Ag(I)/Ag(II): a DFT study.
|
The DFT calculations performed within local density approximation disclose conceivable existence of two novel mixed-valence Ag(I)/Ag(II) fluorides, Ag(2)F(3), i.e., Ag(I)Ag(II)F(3) and Ag(3)F(4), i.e., Ag(I)(2)Ag(II)F(4). Ag(2)F(3) is predicted to crystallize in three equally stable NaCuF(3)-, KAgF(3)-, or CuTeO(3)-type structures, while Ag(3)F(4) should be isostructural to Na(2)CuF(4). The calculated vibration-corrected energies of formation at 0 K of Ag(2)F(3) and Ag(3)F(4) (in their most stable polytypes) from binary fluorides are negative but small (respectively, -0.09 eV and -0.21 eV per formula unit). Formation of Ag(3)F(5) (which, in fact, is a mixed valence Ag(I)/Ag(III) salt) from binary fluorides is much less likely, since the energy of formation is quite positive of about a quarter eV. The predicted volumes per formula unit for all forms of Ag(2)F(3) are larger and that for K(2)CuF(4)-type Ag(3)F(4) is smaller than the sum of volumes of the corresponding binary fluorides; Ag(2)F(3) should not form at high pressure conditions due to a decomposition to the binary constituents. Ag(2)F(3) and Ag(3)F(4) should exhibit genuine mixed- and not intermediate-valence with quite different coordination spheres of Ag(I) and Ag(II). Nevertheless, they should not be electric insulators. Ag(2)F(3) is predicted to be a metallic ferrimagnet with a magnetic superexchange coupling constant, J, of -2 meV while Ag(3)F(4) should be a metallic ferromagnet with J of +52 meV. Since Ag(2)F(3) and Ag(3)F(4) are at the verge of thermodynamic stability, a handful of exothermic reactions have been proposed which could yield these as yet unknown compounds.
|
['Algorithms', 'Computer Simulation', 'Crystallography', 'Electrochemistry', 'Fluorides', 'Models, Chemical', 'Molecular Structure', 'Silver Compounds', 'Thermodynamics']
| 21,258,832
|
[['G17.035', 'L01.224.050'], ['L01.224.160'], ['E05.196.309', 'H01.181.529.240'], ['H01.181.529.307'], ['D01.248.497.158.380', 'D01.303.350.300'], ['E05.599.495'], ['G02.111.570', 'G02.466'], ['D01.847'], ['G01.906']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
|
Role of cranial sutures in normal and abnormal skull development.
|
In a series of experimental studies, the effects of induced premature closure of a normal cranial suture on the normal growth pattern of the rabbit skull are reported. In general, the results indicate that the craniofacial skeleton is an integrated growing unit in which alteration in growth of one component has profound effects on growth of other components. The magnitude and type of compensatory response depend on the type of suture, i.e., cranial or facial, its location, and timing of the growth insult. It is also important to note that the timing of surgical intervention has a direct effect on the capability of the growing craniofacial skeleton to obtain a more typical growth pattern.
|
['Animals', 'Cranial Sutures', 'Rabbits', 'Skull']
| 1,821,301
|
[['B01.050'], ['A02.835.232.781.200'], ['B01.050.150.900.649.313.968.700'], ['A02.835.232.781']]
|
['Organisms [B]', 'Anatomy [A]']
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Expression of equine herpesvirus 1 glycoprotein D by using a recombinant baculovirus.
|
Glycoprotein D (gD) of equine herpesvirus 1 (EHV-1) was expressed at the surface of insect cells infected by a recombinant baculovirus. EHV-1 gD was detected as multiple forms (56, 52, and 48 kDa) from 18 to 96 h postinfection. Laboratory animals inoculated with the recombinant EHV-1 gD developed neutralizing antibody responses against both EHV-1 and EHV-4.
|
['Animals', 'Cloning, Molecular', 'Fluorescent Antibody Technique', 'Herpesvirus 1, Equid', 'Moths', 'Neutralization Tests', 'Nucleopolyhedroviruses', 'Viral Envelope Proteins']
| 8,411,384
|
[['B01.050'], ['E05.393.220'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['B04.280.382.100.900.430'], ['B01.050.500.131.617.720.500.500.937.650'], ['E01.370.225.812.735.550', 'E05.200.812.735.550', 'E05.478.594.760.550'], ['B04.280.065.500', 'B04.525.100.500'], ['D09.400.430.968', 'D12.776.395.550.993', 'D12.776.543.550.993', 'D12.776.964.970.880']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Modeling variation in interaction strength between barnacles and fucoids.
|
The strength by which species interact can vary throughout their ontogeny, as environments vary in space and time, and with the density of their populations. Characterizing strengths of interaction in situ for even a small number of species is logistically difficult and may apply only to those conditions under which the estimates were derived. We sought to combine data from field experiments estimating interaction strength of life stages of the barnacle, Semibalanus balanoides, on germlings of Ascophyllum nodosum, with a model that explored the consequences of variability at per capita and per population levels to the abundance of year-old algal recruits. We further simulated how this interaction affected fucoid germling abundance as the timing of their respective settlements varied relative to one another, as occurs regionally across the Gulf of Maine, USA. Juvenile S. balanoides have a weak estimated per capita effect on germlings. Germling populations are sensitive to variation in per capita effects of juvenile barnacles because of the typically large population sizes of the latter. However, high mortality of juvenile barnacles weakens the population interaction strength over time. Adult barnacles probably weakly facilitate fucoid germlings, but greater survival of adults sustains the strength of that interaction at the population level. Germling abundance is positively associated with densities of adult barnacles and negatively associated with that of juvenile barnacles. Metamorphosing cyprid larvae have the strongest per capita effect on germling abundance, but the interaction between the two stages is so short-lived that germling abundance is altered little. Variation in the timing of barnacle and A. nodosum settlement relative to one another had very little influence on the abundance of yearling germlings. Interactions between barnacles and germlings may influence the demographic structure of A. nodosum populations and the persistence of fucoid-dominated communities on sheltered rocky shores in New England.
|
['Animals', 'Ascophyllum', 'Models, Biological', 'Mortality', 'New England', 'Population Density', 'Population Dynamics', 'Reproduction', 'Thoracica', 'Time Factors']
| 18,975,013
|
[['B01.050'], ['B01.750.600.040'], ['E05.599.395'], ['E05.318.308.985.550', 'N01.224.935.698', 'N06.850.505.400.975.550', 'N06.850.520.308.985.550'], ['Z01.107.567.875.550'], ['N01.224.600', 'N06.850.505.400.600'], ['I01.240.600', 'N01.224.625', 'N06.850.505.400.700'], ['G08.686.784'], ['B01.050.500.131.365.880'], ['G01.910.857']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Phenomena and Processes [G]']
| 0
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
|
Capillary electrophoresis to quantitate gossypol enantiomers in cotton flower petals and seed.
|
Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50ìm, effective length of 24.5cm, 15kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6min.
|
['Electrophoresis, Capillary', 'Flowers', 'Gossypium', 'Gossypol', 'Hydrogen-Ion Concentration', 'Limit of Detection', 'Reproducibility of Results', 'Seeds', 'Spectrophotometry, Ultraviolet', 'Stereoisomerism', 'Temperature']
| 23,122,406
|
[['E05.196.401.190', 'E05.301.300.190'], ['A18.024.249.500'], ['B01.650.940.800.575.912.250.859.821.500.244'], ['D02.455.849.765.444'], ['G02.300'], ['E05.318.740.872.374', 'N05.715.360.750.725.500', 'N06.850.520.830.872.500'], ['E05.318.370.725', 'E05.337.851', 'N05.715.360.325.685', 'N06.850.520.445.725'], ['A18.024.500.750', 'G07.203.300.775', 'J02.500.775'], ['E05.196.712.726.802', 'E05.196.867.826.802'], ['G02.607.445.682'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
Double termination of the alimentary tract in females: a report of 12 cases and a literature review.
|
Twelve female infants with double termination of the alimentary tract were reported. One patient had a high rectovaginal fistula, but in the other 11 cases the tract opened into the bowel uniformly at the level of the levator ani (anorectal-vestibular fistula). In these patients, diagnosis of the anatomical level of the fistula was made definitely with our radiological technique. Excision of not only the fistulous tract but also the anterior half of the rectum below the fistula is essential to achieve a cure without recurrence. The pathogenesis of this condition is discussed and the pertinent literature reviewed.
|
['Anal Canal', 'Female', 'Humans', 'Infant, Newborn', 'Methods', 'Pregnancy', 'Radiography', 'Rectal Fistula', 'Rectovaginal Fistula', 'Rectum', 'Vagina']
| 6,747,793
|
[['A03.556.124.526.070', 'A03.556.249.249.070'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['E05.581'], ['G08.686.784.769'], ['E01.370.350.700'], ['C06.267.550.600', 'C06.405.469.471.600', 'C06.405.469.860.752', 'C23.300.575.185.550.600'], ['C06.267.550.600.650', 'C06.405.469.471.600.650', 'C06.405.469.860.752.650', 'C13.351.500.894.767.249', 'C23.300.575.185.550.600.650', 'C23.300.575.925.558'], ['A03.556.124.526.767', 'A03.556.249.249.767'], ['A05.360.319.779']]
|
['Anatomy [A]', 'Organisms [B]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Association between sphericity, ventricular function and size of the infarction in rats].
|
BACKGROUND: The left ventricular (LV) sphericity is a factor associated with ventricular dysfunction, but it is not well-characterized in the experimental infarction model in rats.OBJECTIVE: To analyze the association between the sphericity index (SI), the ventricular function and the infarcted area in an experimental rat model.METHODS: Six months after the infarction (AMI, n=33) or simulated surgery (SHAM, n=18), the animals were submitted to an echocardiogram. The SI was obtained through the ratio between the diastolic areas at the LV long axis and the short axis.RESULTS: The AMI group presented the lowest index of sphericity (1.32 x 0.23 vs 1.57 x 0.33; p=0.002), systolic function and relative thickness (0.13 x 0.003 vs 0.18 x 0.04; p<0.001) and the highest index of parietal stress (1.27 x 0.33 vs 0.88 x 0.25; p<0.001). There was a significant correlation between the infarct size and sphericity (p=0.046). At the linear regression analysis, the infarct size (p=0.014), but not the sphericity (p=0.683) and the parietal stress (p=0.176), was the predictive factor of the systolic function. Eccentric remodeling (p=0.011), but not sphericity (p=0.183) or the infarct size (p=0.101), was a predictive factor of parietal stress. Additionally, the infarct size (p=0.046), but not the eccentric remodeling (0.705), was a predictive factor of sphericity. The infarct size (p=0.015) and the parietal stress (p=0.011), but not the sphericity (p=0.705), were the predictors of eccentric remodeling.CONCLUSION: The sphericity is associated, but it is not a determinant factor of parietal stress, of eccentric remodeling and ventricular systolic function in an experimental infarction model in rats.
|
['Animals', 'Disease Models, Animal', 'Heart Ventricles', 'Linear Models', 'Male', 'Myocardial Infarction', 'Rats', 'Stress, Mechanical', 'Ventricular Function, Left', 'Ventricular Remodeling']
| 20,379,618
|
[['B01.050'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['A07.541.560'], ['E05.318.740.500.500', 'E05.318.740.750.425', 'E05.599.835.750', 'N05.715.360.750.530.460', 'N05.715.360.750.695.460', 'N06.850.520.830.500.500', 'N06.850.520.830.750.425'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['B01.050.150.900.649.313.992.635.505.700'], ['G01.374.835'], ['G09.330.955.800'], ['C23.300.985', 'G09.330.955.975']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Health Care [N]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.