Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
xet
 Community
1
paragraph_index
int64
sec
string
p_has_citation
int64
cites
string
citeids
list
pmid
int64
cited_id
string
sentences
string
all_sent_cites
list
sent_len
int64
sentence_batch_index
int64
sent_has_citation
float64
qc_fail
bool
cited_sentence
string
cites_in_sentence
list
cln_sentence
string
is_cap
bool
is_alpha
bool
ends_wp
bool
cit_qc
bool
lgtm
bool
__index_level_0__
int64
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
This phenomenon may be attributed to NO-caused impaired nuclear import of APE1 that helped in preventing the already exported APE1 from reimporting into nucleus.
[ "51" ]
161
10,100
0
false
This phenomenon may be attributed to NO-caused impaired nuclear import of APE1 that helped in preventing the already exported APE1 from reimporting into nucleus.
[]
This phenomenon may be attributed to NO-caused impaired nuclear import of APE1 that helped in preventing the already exported APE1 from reimporting into nucleus.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
Different from the APE1-overexpressed cell system, the nuclear-cytosolic shuttling of APE1 may be interfered by some of its binding proteins at normal physiological conditions.
[ "51" ]
176
10,101
0
false
Different from the APE1-overexpressed cell system, the nuclear-cytosolic shuttling of APE1 may be interfered by some of its binding proteins at normal physiological conditions.
[]
Different from the APE1-overexpressed cell system, the nuclear-cytosolic shuttling of APE1 may be interfered by some of its binding proteins at normal physiological conditions.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
It is possible that the protein–protein interactions can repress export of APE1 from nucleus to cytosol.
[ "51" ]
104
10,102
0
false
It is possible that the protein–protein interactions can repress export of APE1 from nucleus to cytosol.
[]
It is possible that the protein–protein interactions can repress export of APE1 from nucleus to cytosol.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
HDAC2 and p50 proteins may act as such intra-nuclear gatekeepers for their ability to antagonize NO-induced APE1 trafficking (Figure 7).
[ "51" ]
136
10,103
0
false
HDAC2 and p50 proteins may act as such intra-nuclear gatekeepers for their ability to antagonize NO-induced APE1 trafficking (Figure 7).
[]
HDAC2 and p50 proteins may act as such intra-nuclear gatekeepers for their ability to antagonize NO-induced APE1 trafficking (Figure 7).
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
The differential intra-nuclear levels of APE1-binding proteins may partly contribute to the cell-type-specific phenomena in APE1 nuclear export.
[ "51" ]
144
10,104
0
false
The differential intra-nuclear levels of APE1-binding proteins may partly contribute to the cell-type-specific phenomena in APE1 nuclear export.
[]
The differential intra-nuclear levels of APE1-binding proteins may partly contribute to the cell-type-specific phenomena in APE1 nuclear export.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
Therefore, the NO-caused APE1 cytosolic translocation may be a complicated process, simultaneously involved in S-nitrosation of Cys93 and Cys310 leading to “NES” exposure, interaction of APE1 with other nuclear proteins and defect in importin nuclear import pathway.
[ "51" ]
266
10,105
0
false
Therefore, the NO-caused APE1 cytosolic translocation may be a complicated process, simultaneously involved in S-nitrosation of Cys93 and Cys310 leading to “NES” exposure, interaction of APE1 with other nuclear proteins and defect in importin nuclear import pathway.
[]
Therefore, the NO-caused APE1 cytosolic translocation may be a complicated process, simultaneously involved in S-nitrosation of Cys93 and Cys310 leading to “NES” exposure, interaction of APE1 with other nuclear proteins and defect in importin nuclear import pathway.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
Figure 8.A model for nuclear-cytosolic translocation of APE1 in response to NO stimulation.
[ "51" ]
91
10,106
0
false
Figure 8.A model for nuclear-cytosolic translocation of APE1 in response to NO stimulation.
[]
Figure 8.A model for nuclear-cytosolic translocation of APE1 in response to NO stimulation.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
APE1 carries an importin-dependent NLS at the N-terminal end (not shown).
[ "51" ]
73
10,107
0
false
APE1 carries an importin-dependent NLS at the N-terminal end (not shown).
[]
APE1 carries an importin-dependent NLS at the N-terminal end (not shown).
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
Two antiparellel beta-strands (B1 and B2) in close proximity to C93 and C310 are masked in the internal structure.
[ "51" ]
114
10,108
0
false
Two antiparellel beta-strands (B1 and B2) in close proximity to C93 and C310 are masked in the internal structure.
[]
Two antiparellel beta-strands in close proximity to C93 and C310 are masked in the internal structure.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
In rested cells, APE1 resides in the nucleus due to the existence of NLS.
[ "51" ]
73
10,109
0
false
In rested cells, APE1 resides in the nucleus due to the existence of NLS.
[]
In rested cells, APE1 resides in the nucleus due to the existence of NLS.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
Upon nitrosative stress, S-nitrosation of C93 and C310 contributes to unmasking of the B1 and/or B2 by changing conformation, which may facilitate the nuclear export of APE1 in a CRM1-independet manner (perhaps mediated by an unknown transport protein).
[ "51" ]
253
10,110
0
false
Upon nitrosative stress, S-nitrosation of C93 and C310 contributes to unmasking of the B1 and/or B2 by changing conformation, which may facilitate the nuclear export of APE1 in a CRM1-independet manner (perhaps mediated by an unknown transport protein).
[]
Upon nitrosative stress, S-nitrosation of C93 and C310 contributes to unmasking of the B1 and/or B2 by changing conformation, which may facilitate the nuclear export of APE1 in a CRM1-independet manner (perhaps mediated by an unknown transport protein).
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
At the same time, importin-dependent nuclear import pathway is repressed by NO insult, which may prevent the already exported cytosolic APE1 from re-import into the nucleus.
[ "51" ]
173
10,111
0
false
At the same time, importin-dependent nuclear import pathway is repressed by NO insult, which may prevent the already exported cytosolic APE1 from re-import into the nucleus.
[]
At the same time, importin-dependent nuclear import pathway is repressed by NO insult, which may prevent the already exported cytosolic APE1 from re-import into the nucleus.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
However, once the intracellular environment becomes reductive (e.g.
[ "51" ]
67
10,112
0
false
However, once the intracellular environment becomes reductive (e.g.
[]
However, once the intracellular environment becomes reductive (e.g.
true
true
true
true
true
1,613
2
DISCUSSION
1
51
[ "B51" ]
17,403,694
pmid-9628873
the increased reducing molecules such as DTT), both NO-elicited S-nitrosation of APE1 and NO-caused repression of importin can be abrogated; thus, the inducible nuclear export of APE1 by NO could be prevented or reversed.
[ "51" ]
221
10,113
0
false
the increased reducing molecules such as DTT), both NO-elicited S-nitrosation of APE1 and NO-caused repression of importin can be abrogated; thus, the inducible nuclear export of APE1 by NO could be prevented or reversed.
[]
the increased reducing molecules such as DTT), both NO-elicited S-nitrosation of APE1 and NO-caused repression of importin can be abrogated; thus, the inducible nuclear export of APE1 by NO could be prevented or reversed.
false
true
true
true
false
1,613
3
DISCUSSION
0
null
null
17,403,694
null
A model for nuclear-cytosolic translocation of APE1 in response to NO stimulation.
null
82
10,114
0
false
null
null
A model for nuclear-cytosolic translocation of APE1 in response to NO stimulation.
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
APE1 carries an importin-dependent NLS at the N-terminal end (not shown).
null
73
10,115
0
false
null
null
APE1 carries an importin-dependent NLS at the N-terminal end (not shown).
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
Two antiparellel beta-strands (B1 and B2) in close proximity to C93 and C310 are masked in the internal structure.
null
114
10,116
0
false
null
null
Two antiparellel beta-strands (B1 and B2) in close proximity to C93 and C310 are masked in the internal structure.
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
In rested cells, APE1 resides in the nucleus due to the existence of NLS.
null
73
10,117
0
false
null
null
In rested cells, APE1 resides in the nucleus due to the existence of NLS.
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
Upon nitrosative stress, S-nitrosation of C93 and C310 contributes to unmasking of the B1 and/or B2 by changing conformation, which may facilitate the nuclear export of APE1 in a CRM1-independet manner (perhaps mediated by an unknown transport protein).
null
253
10,118
0
false
null
null
Upon nitrosative stress, S-nitrosation of C93 and C310 contributes to unmasking of the B1 and/or B2 by changing conformation, which may facilitate the nuclear export of APE1 in a CRM1-independet manner (perhaps mediated by an unknown transport protein).
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
At the same time, importin-dependent nuclear import pathway is repressed by NO insult, which may prevent the already exported cytosolic APE1 from re-import into the nucleus.
null
173
10,119
0
false
null
null
At the same time, importin-dependent nuclear import pathway is repressed by NO insult, which may prevent the already exported cytosolic APE1 from re-import into the nucleus.
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
However, once the intracellular environment becomes reductive (e.g.
null
67
10,120
0
false
null
null
However, once the intracellular environment becomes reductive (e.g.
true
true
true
true
true
1,614
3
DISCUSSION
0
null
null
17,403,694
null
the increased reducing molecules such as DTT), both NO-elicited S-nitrosation of APE1 and NO-caused repression of importin can be abrogated; thus, the inducible nuclear export of APE1 by NO could be prevented or reversed.
null
221
10,121
0
false
null
null
the increased reducing molecules such as DTT), both NO-elicited S-nitrosation of APE1 and NO-caused repression of importin can be abrogated; thus, the inducible nuclear export of APE1 by NO could be prevented or reversed.
false
true
true
true
false
1,614
4
DISCUSSION
1
14
[ "B14" ]
17,403,694
pmid-11554453
In summary, we have reported here that nitric oxide controlled the nuclear export of APE1 through S-nitrosation of Cysteines 93 and 310, which described a novel link between the nitrosative stress and DNA repair/transcriptional regulation system.
[ "14" ]
246
10,122
0
false
In summary, we have reported here that nitric oxide controlled the nuclear export of APE1 through S-nitrosation of Cysteines 93 and 310, which described a novel link between the nitrosative stress and DNA repair/transcriptional regulation system.
[]
In summary, we have reported here that nitric oxide controlled the nuclear export of APE1 through S-nitrosation of Cysteines 93 and 310, which described a novel link between the nitrosative stress and DNA repair/transcriptional regulation system.
true
true
true
true
true
1,615
4
DISCUSSION
1
14
[ "B14" ]
17,403,694
pmid-11554453
It has been reported that redox modification of Cys310 of APE1 can repress its AP endonuclease activity (14).
[ "14" ]
109
10,123
1
false
It has been reported that redox modification of Cys310 of APE1 can repress its AP endonuclease activity.
[ "14" ]
It has been reported that redox modification of Cys310 of APE1 can repress its AP endonuclease activity.
true
true
true
true
true
1,615
4
DISCUSSION
1
14
[ "B14" ]
17,403,694
pmid-11554453
Thus, it is reasonable to expect that NO may exert influence on APE1 DNA repair function through nitrosation of this site.
[ "14" ]
122
10,124
0
false
Thus, it is reasonable to expect that NO may exert influence on APE1 DNA repair function through nitrosation of this site.
[]
Thus, it is reasonable to expect that NO may exert influence on APE1 DNA repair function through nitrosation of this site.
true
true
true
true
true
1,615
4
DISCUSSION
1
14
[ "B14" ]
17,403,694
pmid-11554453
Unraveling the detailed mechanism by which S-nitrosation of APE1 controlled its nuclear export (such as whether a vehicle protein is required for the translocation) and revealing whether S-nitrosation of APE1 can affect its other biological functions present an exciting and relevant challenge for future research.
[ "14" ]
314
10,125
0
false
Unraveling the detailed mechanism by which S-nitrosation of APE1 controlled its nuclear export (such as whether a vehicle protein is required for the translocation) and revealing whether S-nitrosation of APE1 can affect its other biological functions present an exciting and relevant challenge for future research.
[]
Unraveling the detailed mechanism by which S-nitrosation of APE1 controlled its nuclear export (such as whether a vehicle protein is required for the translocation) and revealing whether S-nitrosation of APE1 can affect its other biological functions present an exciting and relevant challenge for future research.
true
true
true
true
true
1,615
4
DISCUSSION
1
14
[ "B14" ]
17,403,694
pmid-11554453
Since NO and APE1 are connected to tumorigenesis and neurodegenerative diseases, the present findings will reasonably improve our understanding of how nitrosative stress leads to DNA damaging at the DNA-repair level and have the potential to yield therapeutic strategies to fight these diseases.
[ "14" ]
295
10,126
0
false
Since NO and APE1 are connected to tumorigenesis and neurodegenerative diseases, the present findings will reasonably improve our understanding of how nitrosative stress leads to DNA damaging at the DNA-repair level and have the potential to yield therapeutic strategies to fight these diseases.
[]
Since NO and APE1 are connected to tumorigenesis and neurodegenerative diseases, the present findings will reasonably improve our understanding of how nitrosative stress leads to DNA damaging at the DNA-repair level and have the potential to yield therapeutic strategies to fight these diseases.
true
true
true
true
true
1,615
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4", "B5", "B6", "B7" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
Opioid receptors (µ, δ and κ) mediate the diverse functions of endogenous opioid peptides and the opioid alkaloids such as morphine, including analgesia, reward, autonomic reflexes, and endocrine/immune regulation (1–3).
[ "1–3", "4", "5", "6", "7" ]
220
10,127
1
false
Opioid receptors (µ, δ and κ) mediate the diverse functions of endogenous opioid peptides and the opioid alkaloids such as morphine, including analgesia, reward, autonomic reflexes, and endocrine/immune regulation.
[ "1–3" ]
Opioid receptors (µ, δ and κ) mediate the diverse functions of endogenous opioid peptides and the opioid alkaloids such as morphine, including analgesia, reward, autonomic reflexes, and endocrine/immune regulation.
true
true
true
true
true
1,616
0
INTRODUCTION
1
4
[ "B1 B2 B3", "B4", "B5", "B6", "B7" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
The mu opioid receptor (MOR) is considered to be the main site of action for morphine (4).
[ "1–3", "4", "5", "6", "7" ]
90
10,128
1
false
The mu opioid receptor (MOR) is considered to be the main site of action for morphine.
[ "4" ]
The mu opioid receptor (MOR) is considered to be the main site of action for morphine.
true
true
true
true
true
1,616
0
INTRODUCTION
1
5
[ "B1 B2 B3", "B4", "B5", "B6", "B7" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
Many studies, including those with receptor knockout mice, have indicated that the responses to opiate agonists are dependent on the receptor level (5).
[ "1–3", "4", "5", "6", "7" ]
152
10,129
1
false
Many studies, including those with receptor knockout mice, have indicated that the responses to opiate agonists are dependent on the receptor level.
[ "5" ]
Many studies, including those with receptor knockout mice, have indicated that the responses to opiate agonists are dependent on the receptor level.
true
true
true
true
true
1,616
0
INTRODUCTION
1
6
[ "B1 B2 B3", "B4", "B5", "B6", "B7" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
MOR is first detected in rat brain at embryonic day 14 (E14) (6) followed by a slight decline during the first week of postnatal development, then increasing to peak levels 2 weeks later (7).
[ "1–3", "4", "5", "6", "7" ]
191
10,130
1
false
MOR is first detected in rat brain at embryonic day 14 followed by a slight decline during the first week of postnatal development, then increasing to peak levels 2 weeks later.
[ "E14", "6", "7" ]
MOR is first detected in rat brain at embryonic day 14 followed by a slight decline during the first week of postnatal development, then increasing to peak levels 2 weeks later.
true
true
true
true
true
1,616
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4", "B5", "B6", "B7" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
The overall mechanisms involved in such spatial and temporal regulations of MOR have not yet been elucidated.
[ "1–3", "4", "5", "6", "7" ]
109
10,131
0
false
The overall mechanisms involved in such spatial and temporal regulations of MOR have not yet been elucidated.
[]
The overall mechanisms involved in such spatial and temporal regulations of MOR have not yet been elucidated.
true
true
true
true
true
1,616
1
INTRODUCTION
1
8
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Generally, transcriptional control is mediated by transcription factors, RNA polymerase and a series of cis-acting elements located in the gene sequences.
[ "8", "9", "10", "11", "10", "12–14", "15" ]
154
10,132
0
false
Generally, transcriptional control is mediated by transcription factors, RNA polymerase and a series of cis-acting elements located in the gene sequences.
[]
Generally, transcriptional control is mediated by transcription factors, RNA polymerase and a series of cis-acting elements located in the gene sequences.
true
true
true
true
true
1,617
1
INTRODUCTION
1
8
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Such promoters, enhancers, silencers and locus-control elements are organized in a modular structure and regulate the production of pre-mRNA molecules (8).
[ "8", "9", "10", "11", "10", "12–14", "15" ]
155
10,133
1
false
Such promoters, enhancers, silencers and locus-control elements are organized in a modular structure and regulate the production of pre-mRNA molecules.
[ "8" ]
Such promoters, enhancers, silencers and locus-control elements are organized in a modular structure and regulate the production of pre-mRNA molecules.
true
true
true
true
true
1,617
1
INTRODUCTION
1
8
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Opioid receptor expression can be regulated by multiple mechanisms, including transcriptional and post-transcriptional events (9,10).
[ "8", "9", "10", "11", "10", "12–14", "15" ]
133
10,134
0
false
Opioid receptor expression can be regulated by multiple mechanisms, including transcriptional and post-transcriptional events.
[ "9,10" ]
Opioid receptor expression can be regulated by multiple mechanisms, including transcriptional and post-transcriptional events.
true
true
true
true
true
1,617
1
INTRODUCTION
1
11
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Our laboratory and others have demonstrated that MOR promoter activity is regulated by many enhancer elements and their related transcriptional factors such as SOX, SP1, AP2, NF-κB, PU.1, and NRSE (11).
[ "8", "9", "10", "11", "10", "12–14", "15" ]
202
10,135
1
false
Our laboratory and others have demonstrated that MOR promoter activity is regulated by many enhancer elements and their related transcriptional factors such as SOX, SP1, AP2, NF-κB, PU.1, and NRSE.
[ "11" ]
Our laboratory and others have demonstrated that MOR promoter activity is regulated by many enhancer elements and their related transcriptional factors such as SOX, SP1, AP2, NF-κB, PU.1, and NRSE.
true
true
true
true
true
1,617
1
INTRODUCTION
1
8
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Post-transcriptional regulation occurs at the level of mRNA or protein.
[ "8", "9", "10", "11", "10", "12–14", "15" ]
71
10,136
0
false
Post-transcriptional regulation occurs at the level of mRNA or protein.
[]
Post-transcriptional regulation occurs at the level of mRNA or protein.
true
true
true
true
true
1,617
1
INTRODUCTION
1
8
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Such regulation could be due to mRNA stability, differences in translation efficiency or mRNA transport, and covalent modification of receptor molecules (10,12–14).
[ "8", "9", "10", "11", "10", "12–14", "15" ]
164
10,137
0
false
Such regulation could be due to mRNA stability, differences in translation efficiency or mRNA transport, and covalent modification of receptor molecules.
[ "10,12–14" ]
Such regulation could be due to mRNA stability, differences in translation efficiency or mRNA transport, and covalent modification of receptor molecules.
true
true
true
true
true
1,617
1
INTRODUCTION
1
15
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Our recent studies indicated that the 3′-untranslated region (UTR) of MOR mRNA could affect the overall transcript's activity (15).
[ "8", "9", "10", "11", "10", "12–14", "15" ]
131
10,138
1
false
Our recent studies indicated that the 3′-untranslated region (UTR) of MOR mRNA could affect the overall transcript's activity.
[ "15" ]
Our recent studies indicated that the 3′-untranslated region (UTR) of MOR mRNA could affect the overall transcript's activity.
true
true
true
true
true
1,617
1
INTRODUCTION
1
8
[ "B8", "B9", "B10", "B11", "B10", "B12 B13 B14", "B15" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
Thus, posttranscriptional regulation of the MOR gene could have an important role in the spatial and temporal expression of the receptor proteins.
[ "8", "9", "10", "11", "10", "12–14", "15" ]
146
10,139
0
false
Thus, posttranscriptional regulation of the MOR gene could have an important role in the spatial and temporal expression of the receptor proteins.
[]
Thus, posttranscriptional regulation of the MOR gene could have an important role in the spatial and temporal expression of the receptor proteins.
true
true
true
true
true
1,617
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
Recently, it has become increasingly clear that the 5′-UTR of eukaryotic mRNA is a key site of multiple forms of post-transcriptional regulation of gene expression, especially those containing
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
192
10,140
0
false
Recently, it has become increasingly clear that the 5′-UTR of eukaryotic mRNA is a key site of multiple forms of post-transcriptional regulation of gene expression, especially those containing
[]
Recently, it has become increasingly clear that the 5′-UTR of eukaryotic mRNA is a key site of multiple forms of post-transcriptional regulation of gene expression, especially those containing
true
true
false
true
false
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
at least one AUG codon (uAUG) upstream of the main open reading frame (ORF) (16–19).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
84
10,141
1
false
at least one AUG codon (uAUG) upstream of the main open reading frame (ORF).
[ "16–19" ]
at least one AUG codon (uAUG) upstream of the main open reading frame (ORF).
false
true
true
true
false
1,618
2
INTRODUCTION
1
20–22
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
It is postulated that, in eukaryotic cells, most translation proceeds according to the ribosome scanning model (20–22), and initiates predominantly by a cap-binding/scanning mechanism (23).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
189
10,142
1
false
It is postulated that, in eukaryotic cells, most translation proceeds according to the ribosome scanning model, and initiates predominantly by a cap-binding/scanning mechanism.
[ "20–22", "23" ]
It is postulated that, in eukaryotic cells, most translation proceeds according to the ribosome scanning model, and initiates predominantly by a cap-binding/scanning mechanism.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The scanning model predicts that an uAUG codon will interfere with translation of a downstream main ORF.
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
104
10,143
0
false
The scanning model predicts that an uAUG codon will interfere with translation of a downstream main ORF.
[]
The scanning model predicts that an uAUG codon will interfere with translation of a downstream main ORF.
true
true
true
true
true
1,618
2
INTRODUCTION
1
24
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
However, the scanning complex may bypass such uAUG codons by ‘leaky scanning’ if the surrounding nucleotide context is suboptimal or very close to the 5′ cap region of the mRNA (24).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
182
10,144
1
false
However, the scanning complex may bypass such uAUG codons by ‘leaky scanning’ if the surrounding nucleotide context is suboptimal or very close to the 5′ cap region of the mRNA.
[ "24" ]
However, the scanning complex may bypass such uAUG codons by ‘leaky scanning’ if the surrounding nucleotide context is suboptimal or very close to the 5′ cap region of the mRNA.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The 43S pre-initiation complex binds to the 5′-cap region and then migrates progressively 5′ to 3′ until it recognizes an AUG start codon.
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
138
10,145
0
false
The 43S pre-initiation complex binds to the 5′-cap region and then migrates progressively 5′ to 3′ until it recognizes an AUG start codon.
[]
The 43S pre-initiation complex binds to the 5′-cap region and then migrates progressively 5′ to 3′ until it recognizes an AUG start codon.
true
true
true
true
true
1,618
2
INTRODUCTION
1
20
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The resulting complex is joined by the large subunit to form a complete ribosome, and polypeptide synthesis begins (20).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
120
10,146
1
false
The resulting complex is joined by the large subunit to form a complete ribosome, and polypeptide synthesis begins.
[ "20" ]
The resulting complex is joined by the large subunit to form a complete ribosome, and polypeptide synthesis begins.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
Ribosomes reaching the main AUG of these mRNAs do so mainly via context-dependent leaky scanning and/or reinitiation mechanisms, although it is widely believed that these are inefficient mechanisms (24,25).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
206
10,147
0
false
Ribosomes reaching the main AUG of these mRNAs do so mainly via context-dependent leaky scanning and/or reinitiation mechanisms, although it is widely believed that these are inefficient mechanisms.
[ "24,25" ]
Ribosomes reaching the main AUG of these mRNAs do so mainly via context-dependent leaky scanning and/or reinitiation mechanisms, although it is widely believed that these are inefficient mechanisms.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The context-dependent leaky scanning mechanism accounts for the observation that some 40S subunits will fail to initiate at AUG codons with a less than optimal context and continue scanning along the 5′-UTR.
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
207
10,148
0
false
The context-dependent leaky scanning mechanism accounts for the observation that some 40S subunits will fail to initiate at AUG codons with a less than optimal context and continue scanning along the 5′-UTR.
[]
The context-dependent leaky scanning mechanism accounts for the observation that some 40S subunits will fail to initiate at AUG codons with a less than optimal context and continue scanning along the 5′-UTR.
true
true
true
true
true
1,618
2
INTRODUCTION
1
26
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The most efficient context for initiation of protein translation is known as the Kozak sequence (GCCRCCAUGG), which was identified initially as a consensus sequence in vertebrate mRNA (26).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
189
10,149
1
false
The most efficient context for initiation of protein translation is known as the Kozak sequence (GCCRCCAUGG), which was identified initially as a consensus sequence in vertebrate mRNA.
[ "26" ]
The most efficient context for initiation of protein translation is known as the Kozak sequence (GCCRCCAUGG), which was identified initially as a consensus sequence in vertebrate mRNA.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The reinitiation mechanism describes the ability of 40S subunits to continue to scan and initiate at a downstream main AUG codon after translating a small independent uORF.
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
172
10,150
0
false
The reinitiation mechanism describes the ability of 40S subunits to continue to scan and initiate at a downstream main AUG codon after translating a small independent uORF.
[]
The reinitiation mechanism describes the ability of 40S subunits to continue to scan and initiate at a downstream main AUG codon after translating a small independent uORF.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
Reinitiation is considered to be a rare event.
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
46
10,151
0
false
Reinitiation is considered to be a rare event.
[]
Reinitiation is considered to be a rare event.
true
true
true
true
true
1,618
2
INTRODUCTION
1
24
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
Although the incidence of this mechanism may be much greater, only a few mRNAs with uORFs have been examined (24).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
114
10,152
1
false
Although the incidence of this mechanism may be much greater, only a few mRNAs with uORFs have been examined.
[ "24" ]
Although the incidence of this mechanism may be much greater, only a few mRNAs with uORFs have been examined.
true
true
true
true
true
1,618
2
INTRODUCTION
1
26
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
These uAUGs or uORFs are features of at least a few percent of the mRNAs in yeast, plants and mammals (26), and can be important players in negative translational control.
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
171
10,153
1
false
These uAUGs or uORFs are features of at least a few percent of the mRNAs in yeast, plants and mammals, and can be important players in negative translational control.
[ "26" ]
These uAUGs or uORFs are features of at least a few percent of the mRNAs in yeast, plants and mammals, and can be important players in negative translational control.
true
true
true
true
true
1,618
2
INTRODUCTION
1
16–19
[ "B16 B17 B18 B19", "B20 B21 B22", "B23", "B24", "B20", "B24", "B25", "B26", "B24", "B26", "B16", "B27" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
However, in some cases, the upstream regulatory sequences stimulated translation of the major ORF (16,27).
[ "16–19", "20–22", "23", "24", "20", "24", "25", "26", "24", "26", "16", "27" ]
106
10,154
0
false
However, in some cases, the upstream regulatory sequences stimulated translation of the major ORF.
[ "16,27" ]
However, in some cases, the upstream regulatory sequences stimulated translation of the major ORF.
true
true
true
true
true
1,618
3
INTRODUCTION
0
null
null
17,284,463
null
Within the 5′-UTR region of the mouse MOR gene, between the basal proximal promoter at nucleotides −445 to −240 and the AUG of the main ORF, three uORFs with variable lengths are identified.
null
190
10,155
0
false
null
null
Within the 5′-UTR region of the mouse MOR gene, between the basal proximal promoter at nucleotides −445 to −240 and the AUG of the main ORF, three uORFs with variable lengths are identified.
true
true
true
true
true
1,619
3
INTRODUCTION
0
null
null
17,284,463
null
Whether these uORFs can regulate the translation of the MOR transcripts was examined in this study.
null
99
10,156
0
false
null
null
Whether these uORFs can regulate the translation of the MOR transcripts was examined in this study.
true
true
true
true
true
1,619
3
INTRODUCTION
0
null
null
17,284,463
null
The uAUG-directed initiation and uORF-peptide-dependent regulations of the MOR transcript's translation were examined by mutational analyses of the uAUGs and internal sequences.
null
177
10,157
0
false
null
null
The uAUG-directed initiation and uORF-peptide-dependent regulations of the MOR transcript's translation were examined by mutational analyses of the uAUGs and internal sequences.
true
true
true
true
true
1,619
3
INTRODUCTION
0
null
null
17,284,463
null
We demonstrate that the translation of the MOR transcript is negatively regulated by these uORFs, and that such down-regulation is mediated via ribosome leaky scanning mechanism.
null
178
10,158
0
false
null
null
We demonstrate that the translation of the MOR transcript is negatively regulated by these uORFs, and that such down-regulation is mediated via ribosome leaky scanning mechanism.
true
true
true
true
true
1,619
3
INTRODUCTION
0
null
null
17,284,463
null
The initiation of peptide syntheses at these uAUGs of the MOR transcript provides a novel mechanism for the regulation of expression of the mouse MOR gene product.
null
163
10,159
0
false
null
null
The initiation of peptide syntheses at these uAUGs of the MOR transcript provides a novel mechanism for the regulation of expression of the mouse MOR gene product.
true
true
true
true
true
1,619
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
The distribution of opioid receptors and the expression patterns of their mRNAs have been extensively examined (39,40).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
119
10,160
0
false
The distribution of opioid receptors and the expression patterns of their mRNAs have been extensively examined.
[ "39,40" ]
The distribution of opioid receptors and the expression patterns of their mRNAs have been extensively examined.
true
true
true
true
true
1,620
0
DISCUSSION
1
10
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
Opioid receptor proteins are also regulated by variations in the mRNA sequences (10).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
85
10,161
1
false
Opioid receptor proteins are also regulated by variations in the mRNA sequences.
[ "10" ]
Opioid receptor proteins are also regulated by variations in the mRNA sequences.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
These mRNA variants differ mainly in the 5′- or 3′-UTR regions.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
63
10,162
0
false
These mRNA variants differ mainly in the 5′- or 3′-UTR regions.
[]
These mRNA variants differ mainly in the 5′- or 3′-UTR regions.
true
true
true
true
true
1,620
0
DISCUSSION
1
11
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
As such, regulation of opioid receptor expression involves not only transcriptional control at the DNA level, but also posttranscriptional control at the RNA level (11).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
169
10,163
1
false
As such, regulation of opioid receptor expression involves not only transcriptional control at the DNA level, but also posttranscriptional control at the RNA level.
[ "11" ]
As such, regulation of opioid receptor expression involves not only transcriptional control at the DNA level, but also posttranscriptional control at the RNA level.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
We have characterized a novel regulatory mechanism of mouse MOR expression mediated by uAUG/uORF in this study.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
111
10,164
0
false
We have characterized a novel regulatory mechanism of mouse MOR expression mediated by uAUG/uORF in this study.
[]
We have characterized a novel regulatory mechanism of mouse MOR expression mediated by uAUG/uORF in this study.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
The 301-bp extension of the mouse MOR 5′-UTR region includes the major MOR mRNA transcription sequence (derived from the proximal MOR promoter), and contains three uORFs.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
170
10,165
0
false
The 301-bp extension of the mouse MOR 5′-UTR region includes the major MOR mRNA transcription sequence (derived from the proximal MOR promoter), and contains three uORFs.
[]
The 301-bp extension of the mouse MOR 5′-UTR region includes the major MOR mRNA transcription sequence (derived from the proximal MOR promoter), and contains three uORFs.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
These uORFs use the same termination codon (Figure 1).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
54
10,166
0
false
These uORFs use the same termination codon (Figure 1).
[]
These uORFs use the same termination codon (Figure 1).
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
In most well-characterized examples of regulatory uORFs, mutation of the uAUG codon alters protein expression without affecting mRNA abundance.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
143
10,167
0
false
In most well-characterized examples of regulatory uORFs, mutation of the uAUG codon alters protein expression without affecting mRNA abundance.
[]
In most well-characterized examples of regulatory uORFs, mutation of the uAUG codon alters protein expression without affecting mRNA abundance.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
However, uORFs do not always inhibit translation; cases have been described in which uORFs stimulated expression of downstream ORFs (41,42).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
140
10,168
0
false
However, uORFs do not always inhibit translation; cases have been described in which uORFs stimulated expression of downstream ORFs.
[ "41,42" ]
However, uORFs do not always inhibit translation; cases have been described in which uORFs stimulated expression of downstream ORFs.
true
true
true
true
true
1,620
0
DISCUSSION
1
16
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
If removal of uAUGs alters mRNA levels, then the AUG nucleotides may alter transcription or RNA stability, independent of translation of the uORF (16).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
151
10,169
1
false
If removal of uAUGs alters mRNA levels, then the AUG nucleotides may alter transcription or RNA stability, independent of translation of the uORF.
[ "16" ]
If removal of uAUGs alters mRNA levels, then the AUG nucleotides may alter transcription or RNA stability, independent of translation of the uORF.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
We found that the presence of the uORF region suppressed translation without changing MOR transcription levels.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
111
10,170
0
false
We found that the presence of the uORF region suppressed translation without changing MOR transcription levels.
[]
We found that the presence of the uORF region suppressed translation without changing MOR transcription levels.
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
This was due to three uAUGs present in the region from nucleotides −301 to +1 bp in the MOR 5′-UTR.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
99
10,171
0
false
This was due to three uAUGs present in the region from nucleotides −301 to +1 bp in the MOR 5′-UTR.
[]
This was due to three uAUGs present in the region from nucleotides −301 to +1 bp in the MOR 5′-UTR.
true
true
true
true
true
1,620
0
DISCUSSION
1
23
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
As uORF mechanisms usually function in a cap-dependent manner (23), we investigated this well-characterized regulatory mechanism by using capped and uncapped transcripts for in vitro translation (Figure 1E and F).
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
213
10,172
1
false
As uORF mechanisms usually function in a cap-dependent manner, we investigated this well-characterized regulatory mechanism by using capped and uncapped transcripts for in vitro translation (Figure 1E and F).
[ "23" ]
As uORF mechanisms usually function in a cap-dependent manner, we investigated this well-characterized regulatory mechanism by using capped and uncapped transcripts for in vitro translation (Figure 1E and F).
true
true
true
true
true
1,620
0
DISCUSSION
1
39
[ "B39", "B40", "B10", "B11", "B41", "B42", "B16", "B23" ]
17,284,463
pmid-8719033|NA|pmid-9437729|NA|pmid-9555078|pmid-9864069|pmid-10836142|NA|NA|pmid-14977578|pmid-15464146|pmid-11106749|pmid-10747993|pmid-11073965|pmid-10395892
We determined that mouse MOR containing uORFs do indeed function in a cap-dependent manner.
[ "39", "40", "10", "11", "41", "42", "16", "23" ]
91
10,173
0
false
We determined that mouse MOR containing uORFs do indeed function in a cap-dependent manner.
[]
We determined that mouse MOR containing uORFs do indeed function in a cap-dependent manner.
true
true
true
true
true
1,620
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
The initiation efficiencies of each uAUG are important features of the codons in MOR 5′-UTR leaky scanning models (43,44).
[ "43", "44" ]
122
10,174
0
false
The initiation efficiencies of each uAUG are important features of the codons in MOR 5′-UTR leaky scanning models.
[ "43,44" ]
The initiation efficiencies of each uAUG are important features of the codons in MOR 5′-UTR leaky scanning models.
true
true
true
true
true
1,621
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
The relative strengths of the initiation codons were tested by in vitro translation (Figure 2).
[ "43", "44" ]
95
10,175
0
false
The relative strengths of the initiation codons were tested by in vitro translation (Figure 2).
[]
The relative strengths of the initiation codons were tested by in vitro translation (Figure 2).
true
true
true
true
true
1,621
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
The uAUG#1 had a very weak initiation context, while uAUG#2 had a slightly stronger initiation context.
[ "43", "44" ]
103
10,176
0
false
The uAUG#1 had a very weak initiation context, while uAUG#2 had a slightly stronger initiation context.
[]
The uAUG#1 had a very weak initiation context, while uAUG#2 had a slightly stronger initiation context.
true
true
true
true
true
1,621
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
The uAUG#3 had the strongest initiation context.
[ "43", "44" ]
48
10,177
0
false
The uAUG#3 had the strongest initiation context.
[]
The uAUG#3 had the strongest initiation context.
true
true
true
true
true
1,621
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
This likely facilitates higher levels of leaky scanning, enabling peptide synthesis from the uAUG#3 initiation codon on the MOR 5′-UTR.
[ "43", "44" ]
135
10,178
0
false
This likely facilitates higher levels of leaky scanning, enabling peptide synthesis from the uAUG#3 initiation codon on the MOR 5′-UTR.
[]
This likely facilitates higher levels of leaky scanning, enabling peptide synthesis from the uAUG#3 initiation codon on the MOR 5′-UTR.
true
true
true
true
true
1,621
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
When the sequence was mutated at all three uAUGs [uAUG(−)], only peptide products initiated from the main uAUG were produced.
[ "43", "44" ]
125
10,179
0
false
When the sequence was mutated at all three uAUGs [uAUG(−)], only peptide products initiated from the main uAUG were produced.
[]
When the sequence was mutated at all three uAUGs [uAUG(−)], only peptide products initiated from the main uAUG were produced.
true
true
true
true
true
1,621
1
DISCUSSION
1
43
[ "B43", "B44" ]
17,284,463
NA|pmid-11786311|pmid-14977578|pmid-15464146|pmid-14977578|pmid-10216946|pmid-11283722|pmid-11416182|pmid-15879516|pmid-7687304|pmid-1566578
These results indicate that all three uAUGs can effectively be initiated and produce peptides, and that uORFs can negatively regulate downstream MOR ORF initiation.
[ "43", "44" ]
164
10,180
0
false
These results indicate that all three uAUGs can effectively be initiated and produce peptides, and that uORFs can negatively regulate downstream MOR ORF initiation.
[]
These results indicate that all three uAUGs can effectively be initiated and produce peptides, and that uORFs can negatively regulate downstream MOR ORF initiation.
true
true
true
true
true
1,621
2
DISCUSSION
1
45
[ "B45", "B46" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
We also performed FACS analyses and receptor binding assays to examine cell surface expression of the receptor (45,46).
[ "45", "46" ]
119
10,181
0
false
We also performed FACS analyses and receptor binding assays to examine cell surface expression of the receptor.
[ "45,46" ]
We also performed FACS analyses and receptor binding assays to examine cell surface expression of the receptor.
true
true
true
true
true
1,622
2
DISCUSSION
1
45
[ "B45", "B46" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
The results confirmed that these uORFs mediated down-regulation of MOR expression.
[ "45", "46" ]
82
10,182
0
false
The results confirmed that these uORFs mediated down-regulation of MOR expression.
[]
The results confirmed that these uORFs mediated down-regulation of MOR expression.
true
true
true
true
true
1,622
2
DISCUSSION
1
45
[ "B45", "B46" ]
17,284,463
pmid-11073965|pmid-11718907|pmid-11164040|pmid-14990743|pmid-12435632|pmid-681367|pmid-2645293|pmid-10395892|pmid-12799445|pmid-12435632|pmid-12799445|pmid-12459250|pmid-1955461|pmid-12799445|pmid-1955461|pmid-11073965|pmid-12890013|pmid-15657030|pmid-12851402
Indeed, the presence of the uORFs decreased expression by up to 3.5-fold relative to samples lacking the uORFs (Figure 3).
[ "45", "46" ]
122
10,183
0
false
Indeed, the presence of the uORFs decreased expression by up to 3.5-fold relative to samples lacking the uORFs (Figure 3).
[]
Indeed, the presence of the uORFs decreased expression by up to 3.5-fold relative to samples lacking the uORFs (Figure 3).
true
true
true
true
true
1,622
3
DISCUSSION
0
null
null
17,284,463
null
We further confirmed the context-dependent initiation activity of each uAUG by mutation.
null
88
10,184
0
false
null
null
We further confirmed the context-dependent initiation activity of each uAUG by mutation.
true
true
true
true
true
1,623
3
DISCUSSION
0
null
null
17,284,463
null
Translation initiation strength was most efficient in the third uAUG (Figure 4B).
null
81
10,185
0
false
null
null
Translation initiation strength was most efficient in the third uAUG (Figure 4B).
true
true
true
true
true
1,623
3
DISCUSSION
0
null
null
17,284,463
null
If the uAUGs reside in a non-optimal context, the scanning ribosomal complex may bypass possible starting AUGs by leaky scanning.
null
129
10,186
0
false
null
null
If the uAUGs reside in a non-optimal context, the scanning ribosomal complex may bypass possible starting AUGs by leaky scanning.
true
true
true
true
true
1,623
3
DISCUSSION
0
null
null
17,284,463
null
Transient transfection results suggest that the inhibition of the main AUG imposed by one of the uORF (uAUG#3) was greater than that produced by the other uORFs (uAUG#1 or uAUG#2).
null
180
10,187
0
false
null
null
Transient transfection results suggest that the inhibition of the main AUG imposed by one of the uORF (uAUG#3) was greater than that produced by the other uORFs (uAUG#1 or uAUG#2).
true
true
true
true
true
1,623
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
Moreover, some peptides produced by translation of uORFs have been reported to interact with the ribosome, further diminishing the efficiency of protein translation (47,48).
[ "47", "48", "49", "50", "51" ]
173
10,188
0
false
Moreover, some peptides produced by translation of uORFs have been reported to interact with the ribosome, further diminishing the efficiency of protein translation.
[ "47,48" ]
Moreover, some peptides produced by translation of uORFs have been reported to interact with the ribosome, further diminishing the efficiency of protein translation.
true
true
true
true
true
1,624
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
In contrast, regulation of yeast GCN4 translation by nutrient levels was independent of the peptide sequences encoded by its uORFs (49,50).
[ "47", "48", "49", "50", "51" ]
139
10,189
0
false
In contrast, regulation of yeast GCN4 translation by nutrient levels was independent of the peptide sequences encoded by its uORFs.
[ "49,50" ]
In contrast, regulation of yeast GCN4 translation by nutrient levels was independent of the peptide sequences encoded by its uORFs.
true
true
true
true
true
1,624
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
Our current study showed that introduction of stop codon constructs (+S), thereby changing the sequence of peptide encoded by the uORF, exhibited changes similar to those seen in the translation of the downstream cistron (Figure 5B).
[ "47", "48", "49", "50", "51" ]
233
10,190
0
false
Our current study showed that introduction of stop codon constructs (+S), thereby changing the sequence of peptide encoded by the uORF, exhibited changes similar to those seen in the translation of the downstream cistron (Figure 5B).
[]
Our current study showed that introduction of stop codon constructs (+S), thereby changing the sequence of peptide encoded by the uORF, exhibited changes similar to those seen in the translation of the downstream cistron (Figure 5B).
true
true
true
true
true
1,624
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
Furthermore, mutating the uAUGs while also introducing the stop codon (M + S) caused a marked increase in the translation of the downstream cistron (Figure 5D).
[ "47", "48", "49", "50", "51" ]
160
10,191
0
false
Furthermore, mutating the uAUGs while also introducing the stop codon (M + S) caused a marked increase in the translation of the downstream cistron (Figure 5D).
[]
Furthermore, mutating the uAUGs while also introducing the stop codon (M + S) caused a marked increase in the translation of the downstream cistron (Figure 5D).
true
true
true
true
true
1,624
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
These data show that regulation of the MOR uORF is independent of the peptide sequence of the uORF.
[ "47", "48", "49", "50", "51" ]
99
10,192
0
false
These data show that regulation of the MOR uORF is independent of the peptide sequence of the uORF.
[]
These data show that regulation of the MOR uORF is independent of the peptide sequence of the uORF.
true
true
true
true
true
1,624
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
Thus, the inhibitory effect of the uORF during MOR translation is a protein-independent mechanism.
[ "47", "48", "49", "50", "51" ]
98
10,193
0
false
Thus, the inhibitory effect of the uORF during MOR translation is a protein-independent mechanism.
[]
Thus, the inhibitory effect of the uORF during MOR translation is a protein-independent mechanism.
true
true
true
true
true
1,624
4
DISCUSSION
1
47
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
Although the mechanisms involved in explaining the inhibitory effects of the uORF peptide on translation are not understood, several models could be proposed.
[ "47", "48", "49", "50", "51" ]
158
10,194
0
false
Although the mechanisms involved in explaining the inhibitory effects of the uORF peptide on translation are not understood, several models could be proposed.
[]
Although the mechanisms involved in explaining the inhibitory effects of the uORF peptide on translation are not understood, several models could be proposed.
true
true
true
true
true
1,624
4
DISCUSSION
1
51
[ "B47", "B48", "B49", "B50", "B51" ]
17,284,463
pmid-11328869|pmid-12228716|NA|pmid-11707416|pmid-8308019
For example, the peptide of the uORF could be synthesized and have the ability to inhibit translation only at high concentrations in the local microenvironment (51).
[ "47", "48", "49", "50", "51" ]
165
10,195
1
false
For example, the peptide of the uORF could be synthesized and have the ability to inhibit translation only at high concentrations in the local microenvironment.
[ "51" ]
For example, the peptide of the uORF could be synthesized and have the ability to inhibit translation only at high concentrations in the local microenvironment.
true
true
true
true
true
1,624
5
DISCUSSION
0
null
null
17,284,463
null
As shown in Figure 6, differences in LUC levels were detected between constructs with different uAUG contexts.
null
110
10,196
0
false
null
null
As shown in Figure 6, differences in LUC levels were detected between constructs with different uAUG contexts.
true
true
true
true
true
1,625
5
DISCUSSION
0
null
null
17,284,463
null
This indicates that ribosomes initiating at the main AUG must have scanned the uAUGs, especially when these uAUGs contain strong or adequate contexts.
null
150
10,197
0
false
null
null
This indicates that ribosomes initiating at the main AUG must have scanned the uAUGs, especially when these uAUGs contain strong or adequate contexts.
true
true
true
true
true
1,625
5
DISCUSSION
0
null
null
17,284,463
null
Leaky scanning is an extension of the scanning model and suggests that an AUG might be bypassed by scanning ribosomes if it resides in a suboptimal context.
null
156
10,198
0
false
null
null
Leaky scanning is an extension of the scanning model and suggests that an AUG might be bypassed by scanning ribosomes if it resides in a suboptimal context.
true
true
true
true
true
1,625
5
DISCUSSION
0
null
null
17,284,463
null
This mechanism could only occur by context-dependent leaky scanning.
null
68
10,199
0
false
null
null
This mechanism could only occur by context-dependent leaky scanning.
true
true
true
true
true
1,625