Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
xet
 Community
1
paragraph_index
int64
sec
string
p_has_citation
int64
cites
string
citeids
list
pmid
int64
cited_id
string
sentences
string
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list
sent_len
int64
sentence_batch_index
int64
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bool
cited_sentence
string
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bool
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__index_level_0__
int64
4
DISCUSSION
0
null
null
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-11741897
These complexes repress ribonuclease activity of Kid, but allow efficient transcription.
null
88
5,000
0
false
null
null
These complexes repress ribonuclease activity of Kid, but allow efficient transcription.
true
true
true
true
true
834
4
DISCUSSION
0
null
null
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-11741897
When the concentration of Kid is equal or lower than that of Kis, mostly 1:1 complexes are formed.
null
98
5,001
0
false
null
null
When the concentration of Kid is equal or lower than that of Kis, mostly 1:1 complexes are formed.
true
true
true
true
true
834
4
DISCUSSION
0
null
null
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-11741897
These complexes do not only repress the ribonuclease activity of Kid, but also the transcription process.
null
105
5,002
0
false
null
null
These complexes do not only repress the ribonuclease activity of Kid, but also the transcription process.
true
true
true
true
true
834
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
Schematic model of the transcription autoregulation of the parD operon.
null
71
5,003
0
false
null
null
Schematic model of the transcription autoregulation of the parD operon.
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
The kid gene and the Kid protein are shown in blue and the kis gene and the Kis protein in orange.
null
98
5,004
0
false
null
null
The kid gene and the Kid protein are shown in blue and the kis gene and the Kis protein in orange.
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
Each protein complex is represented by an appropriate combination of blue rectangles (Kid) and orange ellipses (Kis).
null
117
5,005
0
false
null
null
Each protein complex is represented by an appropriate combination of blue rectangles (Kid) and orange ellipses (Kis).
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
Free Kid inhibits cell growth.
null
30
5,006
0
false
null
null
Free Kid inhibits cell growth.
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
In conditions in which the concentration of Kid is higher than that of Kis Kid2–Kis1 and Kid2–Kis2–Kid2 complexes are formed.
null
125
5,007
0
false
null
null
In conditions in which the concentration of Kid is higher than that of Kis Kid2–Kis1 and Kid2–Kis2–Kid2 complexes are formed.
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
These complexes repress ribonuclease activity of Kid, but allow efficient transcription.
null
88
5,008
0
false
null
null
These complexes repress ribonuclease activity of Kid, but allow efficient transcription.
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
When the concentration of Kid is equal or lower than that of Kis, mostly 1:1 complexes are formed.
null
98
5,009
0
false
null
null
When the concentration of Kid is equal or lower than that of Kis, mostly 1:1 complexes are formed.
true
true
true
true
true
835
5
DISCUSSION
0
null
null
17,317,682
pmid-15056667|NA|pmid-16869714|pmid-17073415|pmid-16594706
These complexes do not only repress the ribonuclease activity of Kid, but also the transcription process.
null
105
5,010
0
false
null
null
These complexes do not only repress the ribonuclease activity of Kid, but also the transcription process.
true
true
true
true
true
835
6
DISCUSSION
1
23–25
[ "B23 B24 B25", "B37", "B25", "B37", "B4", "B10", "B18" ]
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-17007877|pmid-11454201|pmid-17007877|pmid-1324324|pmid-15838042|pmid-16413033
Similar mechanisms of transcription autoregulation have been proposed for the ccd and the mazEF addiction systems (23–25,37).
[ "23–25", "37", "25", "37", "4", "10", "18" ]
125
5,011
0
false
Similar mechanisms of transcription autoregulation have been proposed for the ccd and the mazEF addiction systems.
[ "23–25,37" ]
Similar mechanisms of transcription autoregulation have been proposed for the ccd and the mazEF addiction systems.
true
true
true
true
true
836
6
DISCUSSION
1
25
[ "B23 B24 B25", "B37", "B25", "B37", "B4", "B10", "B18" ]
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-17007877|pmid-11454201|pmid-17007877|pmid-1324324|pmid-15838042|pmid-16413033
Electrophoretic mobility shift assays have shown that multimers of CcdA2CcdB2 have multiple DNA-binding sites and spirals around the promoter region (25).
[ "23–25", "37", "25", "37", "4", "10", "18" ]
154
5,012
1
false
Electrophoretic mobility shift assays have shown that multimers of CcdA2CcdB2 have multiple DNA-binding sites and spirals around the promoter region.
[ "25" ]
Electrophoretic mobility shift assays have shown that multimers of CcdA2CcdB2 have multiple DNA-binding sites and spirals around the promoter region.
true
true
true
true
true
836
6
DISCUSSION
1
23–25
[ "B23 B24 B25", "B37", "B25", "B37", "B4", "B10", "B18" ]
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-17007877|pmid-11454201|pmid-17007877|pmid-1324324|pmid-15838042|pmid-16413033
It has also been proposed that when CcdB is present in a molar excess over CcdA, binding of a CcdB2 dimer to a (CcdB2-CcdA2) – DNA complex causes steric hindrance and, therefore, loosens the interaction of the protein complex with DNA.
[ "23–25", "37", "25", "37", "4", "10", "18" ]
235
5,013
0
false
It has also been proposed that when CcdB is present in a molar excess over CcdA, binding of a CcdB2 dimer to a (CcdB2-CcdA2) – DNA complex causes steric hindrance and, therefore, loosens the interaction of the protein complex with DNA.
[]
It has also been proposed that when CcdB is present in a molar excess over CcdA, binding of a CcdB2 dimer to a – DNA complex causes steric hindrance and, therefore, loosens the interaction of the protein complex with DNA.
true
true
true
true
true
836
6
DISCUSSION
1
37
[ "B23 B24 B25", "B37", "B25", "B37", "B4", "B10", "B18" ]
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-17007877|pmid-11454201|pmid-17007877|pmid-1324324|pmid-15838042|pmid-16413033
This will alleviate the inhibition of transcription (37).
[ "23–25", "37", "25", "37", "4", "10", "18" ]
57
5,014
1
false
This will alleviate the inhibition of transcription.
[ "37" ]
This will alleviate the inhibition of transcription.
true
true
true
true
true
836
6
DISCUSSION
1
23–25
[ "B23 B24 B25", "B37", "B25", "B37", "B4", "B10", "B18" ]
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-17007877|pmid-11454201|pmid-17007877|pmid-1324324|pmid-15838042|pmid-16413033
On the other hand, for the parD system, the binding of Kis or Kid–Kis complexes to the parD operator-promoter region occurs only in two discrete regions (I and II) spaced by 33 bp; the distribution of the specific contacts that Kis and Kid–Kis complexes make on each of the DNA regions are spaced 11–13 bp, indicating th...
[ "23–25", "37", "25", "37", "4", "10", "18" ]
369
5,015
0
false
On the other hand, for the parD system, the binding of Kis or Kid–Kis complexes to the parD operator-promoter region occurs only in two discrete regions (I and II) spaced by 33 bp; the distribution of the specific contacts that Kis and Kid–Kis complexes make on each of the DNA regions are spaced 11–13 bp, indicating th...
[]
On the other hand, for the parD system, the binding of Kis or Kid–Kis complexes to the parD operator-promoter region occurs only in two discrete regions (I and II) spaced by 33 bp; the distribution of the specific contacts that Kis and Kid–Kis complexes make on each of the DNA regions are spaced 11–13 bp, indicating th...
true
true
true
true
true
836
6
DISCUSSION
1
4
[ "B23 B24 B25", "B37", "B25", "B37", "B4", "B10", "B18" ]
17,317,682
pmid-11741897|pmid-12718874|pmid-11454201|pmid-17007877|pmid-11454201|pmid-17007877|pmid-1324324|pmid-15838042|pmid-16413033
It should be noted here that CcdB and Kid have different activities: CcdB acts as a toxin and inhibitor for DNA gyrase, an essential enzyme that catalyses negative supercoiling of DNA (4), whereas Kid functions as a ribosome-independent RNase (10,18).
[ "23–25", "37", "25", "37", "4", "10", "18" ]
251
5,016
1
false
It should be noted here that CcdB and Kid have different activities: CcdB acts as a toxin and inhibitor for DNA gyrase, an essential enzyme that catalyses negative supercoiling of DNA, whereas Kid functions as a ribosome-independent RNase.
[ "4", "10,18" ]
It should be noted here that CcdB and Kid have different activities: CcdB acts as a toxin and inhibitor for DNA gyrase, an essential enzyme that catalyses negative supercoiling of DNA, whereas Kid functions as a ribosome-independent RNase.
true
true
true
true
true
836
7
DISCUSSION
1
38
[ "B38" ]
17,317,682
pmid-16257530
Although no stoichiometric complexes of MazF–MazE on the DNA have been identified, it has been postulated that MazE mediates assembly of heterocomplexes on DNA.
[ "38" ]
160
5,017
0
false
Although no stoichiometric complexes of MazF–MazE on the DNA have been identified, it has been postulated that MazE mediates assembly of heterocomplexes on DNA.
[]
Although no stoichiometric complexes of MazF–MazE on the DNA have been identified, it has been postulated that MazE mediates assembly of heterocomplexes on DNA.
true
true
true
true
true
837
7
DISCUSSION
1
38
[ "B38" ]
17,317,682
pmid-16257530
The resulting higher order complexes would then form stoichiometric MazF–MazE complexes.
[ "38" ]
88
5,018
0
false
The resulting higher order complexes would then form stoichiometric MazF–MazE complexes.
[]
The resulting higher order complexes would then form stoichiometric MazF–MazE complexes.
true
true
true
true
true
837
7
DISCUSSION
1
38
[ "B38" ]
17,317,682
pmid-16257530
In the mazEF system, the promoter region contains three antitoxin-binding regions (11–12-bp long) that can form an ‘alternating palindrome’.
[ "38" ]
140
5,019
0
false
In the mazEF system, the promoter region contains three antitoxin-binding regions (11–12-bp long) that can form an ‘alternating palindrome’.
[]
In the mazEF system, the promoter region contains three antitoxin-binding regions (11–12-bp long) that can form an ‘alternating palindrome’.
true
true
true
true
true
837
7
DISCUSSION
1
38
[ "B38" ]
17,317,682
pmid-16257530
It has been proposed that MazE antitoxin binds to these sites and that two MazF dimers can bridge the MazE dimers in a highly cooperative interaction (38).
[ "38" ]
155
5,020
1
false
It has been proposed that MazE antitoxin binds to these sites and that two MazF dimers can bridge the MazE dimers in a highly cooperative interaction.
[ "38" ]
It has been proposed that MazE antitoxin binds to these sites and that two MazF dimers can bridge the MazE dimers in a highly cooperative interaction.
true
true
true
true
true
837
7
DISCUSSION
1
38
[ "B38" ]
17,317,682
pmid-16257530
The situation in the kid–kis system is different in that the promoter region contains two binding regions, I and II, of 18-bp.
[ "38" ]
126
5,021
0
false
The situation in the kid–kis system is different in that the promoter region contains two binding regions, I and II, of 18-bp.
[]
The situation in the kid–kis system is different in that the promoter region contains two binding regions, I and II, of 18-bp.
true
true
true
true
true
837
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
In Drosophila melanogaster, as in many animals, the female state is defined by the presence of two X chromosomes, but males contain only a single X chromosome in addition to the gene-poor Y chromosome.
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
201
5,022
0
false
In Drosophila melanogaster, as in many animals, the female state is defined by the presence of two X chromosomes, but males contain only a single X chromosome in addition to the gene-poor Y chromosome.
[]
In Drosophila melanogaster, as in many animals, the female state is defined by the presence of two X chromosomes, but males contain only a single X chromosome in addition to the gene-poor Y chromosome.
true
true
true
true
true
838
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
A priori, the genome of male fruit flies appears unbalanced due to the halved dosage of X chromosomal genes.
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
108
5,023
0
false
A priori, the genome of male fruit flies appears unbalanced due to the halved dosage of X chromosomal genes.
[]
A priori, the genome of male fruit flies appears unbalanced due to the halved dosage of X chromosomal genes.
true
true
true
true
true
838
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Re-establishment of proper balance requires a compensatory mechanism that raises the expression levels of the single male X chromosome to match the expression from the two X chromosomes in females (1–3).
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
203
5,024
1
false
Re-establishment of proper balance requires a compensatory mechanism that raises the expression levels of the single male X chromosome to match the expression from the two X chromosomes in females.
[ "1–3" ]
Re-establishment of proper balance requires a compensatory mechanism that raises the expression levels of the single male X chromosome to match the expression from the two X chromosomes in females.
true
true
true
true
true
838
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Failure of such ‘dosage compensation’ is lethal to the affected males (for reviews see (4–8)).
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
94
5,025
0
false
Failure of such ‘dosage compensation’ is lethal to the affected males ).
[ "for reviews see (4–8" ]
Failure of such ‘dosage compensation’ is lethal to the affected males ).
true
true
true
true
true
838
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Dosage compensation in flies is achieved through a male-specific ribonucleoprotein complex, the Dosage Compensation Complex (DCC, also known as the Male Specific Lethal or MSL complex), which is able to distinguish the X chromosome from the autosomes and to bind the X selectively.
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
281
5,026
0
false
Dosage compensation in flies is achieved through a male-specific ribonucleoprotein complex, the Dosage Compensation Complex (DCC, also known as the Male Specific Lethal or MSL complex), which is able to distinguish the X chromosome from the autosomes and to bind the X selectively.
[]
Dosage compensation in flies is achieved through a male-specific ribonucleoprotein complex, the Dosage Compensation Complex (DCC, also known as the Male Specific Lethal or MSL complex), which is able to distinguish the X chromosome from the autosomes and to bind the X selectively.
true
true
true
true
true
838
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
The DCC consists of five core proteins (MSL1, -2, -3, MOF and MLE) and two non-coding RNAs, roX1 and roX2 (for review see (8) and references therein).
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
150
5,027
0
false
The DCC consists of five core proteins (MSL1, -2, -3, MOF and MLE) and two non-coding RNAs, roX1 and roX2 and references therein).
[ "for review see (8" ]
The DCC consists of five core proteins and two non-coding RNAs, roX1 and roX2 and references therein).
true
true
true
true
true
838
0
INTRODUCTION
1
9–11
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Gene activation involves modification of X chromosomal chromatin by the DCC-associated acetyltransferase MOF, which acetylates histone H4 at lysine 16 (9–11), but contributions from more general factors, such as the H3 serine 10 kinase Jil1 (12,13), the supercoiling factor (14) and nuclear pore components (15) have bee...
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
332
5,028
1
false
Gene activation involves modification of X chromosomal chromatin by the DCC-associated acetyltransferase MOF, which acetylates histone H4 at lysine 16, but contributions from more general factors, such as the H3 serine 10 kinase Jil1, the supercoiling factor and nuclear pore components have been suggested.
[ "9–11", "12,13", "14", "15" ]
Gene activation involves modification of X chromosomal chromatin by the DCC-associated acetyltransferase MOF, which acetylates histone H4 at lysine 16, but contributions from more general factors, such as the H3 serine 10 kinase Jil1, the supercoiling factor and nuclear pore components have been suggested.
true
true
true
true
true
838
0
INTRODUCTION
1
16–18
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
The targeting of these effects to the X chromosome relies primarily on MSL1 and MSL2, the two DCC proteins that are able to recognize a subset of sites on the X chromosome even in the absence of all other factors (16–18).
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
221
5,029
1
false
The targeting of these effects to the X chromosome relies primarily on MSL1 and MSL2, the two DCC proteins that are able to recognize a subset of sites on the X chromosome even in the absence of all other factors.
[ "16–18" ]
The targeting of these effects to the X chromosome relies primarily on MSL1 and MSL2, the two DCC proteins that are able to recognize a subset of sites on the X chromosome even in the absence of all other factors.
true
true
true
true
true
838
0
INTRODUCTION
1
1–3
[ "B1 B2 B3", "B4 B5 B6 B7 B8", "B8", "B9 B10 B11", "B12", "B13", "B14", "B15", "B16 B17 B18", "B19", "B20" ]
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
However, faithful occupancy of all sites on the X chromosome requires additional factors, such as the activities of the acetyltransferase MOF and the RNA helicase MLE, as well as the presence of the roX RNAs (19,20).
[ "1–3", "4–8", "8", "9–11", "12", "13", "14", "15", "16–18", "19", "20" ]
216
5,030
0
false
However, faithful occupancy of all sites on the X chromosome requires additional factors, such as the activities of the acetyltransferase MOF and the RNA helicase MLE, as well as the presence of the roX RNAs.
[ "19,20" ]
However, faithful occupancy of all sites on the X chromosome requires additional factors, such as the activities of the acetyltransferase MOF and the RNA helicase MLE, as well as the presence of the roX RNAs.
true
true
true
true
true
838
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
How the DCC recognizes a single chromosome is a question of great interest.
[ "21", "22", "23", "21", "22", "21", "22" ]
75
5,031
0
false
How the DCC recognizes a single chromosome is a question of great interest.
[]
How the DCC recognizes a single chromosome is a question of great interest.
true
true
true
true
true
839
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
Ultimately, recognition must involve X-specific DNA sequences.
[ "21", "22", "23", "21", "22", "21", "22" ]
62
5,032
0
false
Ultimately, recognition must involve X-specific DNA sequences.
[]
Ultimately, recognition must involve X-specific DNA sequences.
true
true
true
true
true
839
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
Combining chromatin immunoprecipitation (ChIP) with probing of high-density oligonucleotide arrays ‘tiling’ the entire X chromosome, the interaction of the DCC with the X chromosome has recently been mapped (21,22).
[ "21", "22", "23", "21", "22", "21", "22" ]
215
5,033
0
false
Combining chromatin immunoprecipitation (ChIP) with probing of high-density oligonucleotide arrays ‘tiling’ the entire X chromosome, the interaction of the DCC with the X chromosome has recently been mapped.
[ "21,22" ]
Combining chromatin immunoprecipitation (ChIP) with probing of high-density oligonucleotide arrays ‘tiling’ the entire X chromosome, the interaction of the DCC with the X chromosome has recently been mapped.
true
true
true
true
true
839
1
INTRODUCTION
1
23
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
These studies revealed that about 25% of the X chromosomal DNA is bound by DCC in tissue culture cells or Drosophila embryos and, notably, the majority of DCC binding is found within coding sequences, reaffirming an earlier suggestion that the DCC may act to facilitate transcription elongation rather than initiation (2...
[ "21", "22", "23", "21", "22", "21", "22" ]
323
5,034
1
false
These studies revealed that about 25% of the X chromosomal DNA is bound by DCC in tissue culture cells or Drosophila embryos and, notably, the majority of DCC binding is found within coding sequences, reaffirming an earlier suggestion that the DCC may act to facilitate transcription elongation rather than initiation.
[ "23" ]
These studies revealed that about 25% of the X chromosomal DNA is bound by DCC in tissue culture cells or Drosophila embryos and, notably, the majority of DCC binding is found within coding sequences, reaffirming an earlier suggestion that the DCC may act to facilitate transcription elongation rather than initiation.
true
true
true
true
true
839
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
However, despite this wealth of interaction data it has not been possible to distil a set of ‘consensus’ DNA sequences that define DCC binding (21,22).
[ "21", "22", "23", "21", "22", "21", "22" ]
151
5,035
0
false
However, despite this wealth of interaction data it has not been possible to distil a set of ‘consensus’ DNA sequences that define DCC binding.
[ "21,22" ]
However, despite this wealth of interaction data it has not been possible to distil a set of ‘consensus’ DNA sequences that define DCC binding.
true
true
true
true
true
839
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
It therefore remains possible that the observed X chromosomal binding pattern of the DCC is governed by more than just DNA sequence.
[ "21", "22", "23", "21", "22", "21", "22" ]
132
5,036
0
false
It therefore remains possible that the observed X chromosomal binding pattern of the DCC is governed by more than just DNA sequence.
[]
It therefore remains possible that the observed X chromosomal binding pattern of the DCC is governed by more than just DNA sequence.
true
true
true
true
true
839
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
DNA sequence may just define a subset of ‘primary’ targeting sites from which the DCC is distributed to secondary sites in neighbouring chromatin.
[ "21", "22", "23", "21", "22", "21", "22" ]
146
5,037
0
false
DNA sequence may just define a subset of ‘primary’ targeting sites from which the DCC is distributed to secondary sites in neighbouring chromatin.
[]
DNA sequence may just define a subset of ‘primary’ targeting sites from which the DCC is distributed to secondary sites in neighbouring chromatin.
true
true
true
true
true
839
1
INTRODUCTION
1
21
[ "B21", "B22", "B23", "B21", "B22", "B21", "B22" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
Because the DCC interacts preferentially with active genes it is possible that the process of transcription itself or a transcription-associated epigenetic modification of chromatin generates secondary sites (21,22).
[ "21", "22", "23", "21", "22", "21", "22" ]
216
5,038
0
false
Because the DCC interacts preferentially with active genes it is possible that the process of transcription itself or a transcription-associated epigenetic modification of chromatin generates secondary sites.
[ "21,22" ]
Because the DCC interacts preferentially with active genes it is possible that the process of transcription itself or a transcription-associated epigenetic modification of chromatin generates secondary sites.
true
true
true
true
true
839
2
INTRODUCTION
1
24
[ "B24", "B25", "B25", "B25", "B26" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
While the nature of those presumed secondary sites is entirely unclear, the existence of primary sites, defined by DNA sequences with autonomous DCC recruitment activity, has been inferred from P-element-mediated insertion of X-derived sequences into autosomes, where association of the DCC with these ectopic sites can ...
[ "24", "25", "25", "25", "26" ]
382
5,039
0
false
While the nature of those presumed secondary sites is entirely unclear, the existence of primary sites, defined by DNA sequences with autonomous DCC recruitment activity, has been inferred from P-element-mediated insertion of X-derived sequences into autosomes, where association of the DCC with these ectopic sites can ...
[]
While the nature of those presumed secondary sites is entirely unclear, the existence of primary sites, defined by DNA sequences with autonomous DCC recruitment activity, has been inferred from P-element-mediated insertion of X-derived sequences into autosomes, where association of the DCC with these ectopic sites can ...
true
true
true
true
true
840
2
INTRODUCTION
1
24
[ "B24", "B25", "B25", "B25", "B26" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
The first DCC binding sites characterized in this way correspond to ∼200 bp sequences found in the coding regions of the roX1 and roX2 genes (24,25).
[ "24", "25", "25", "25", "26" ]
149
5,040
0
false
The first DCC binding sites characterized in this way correspond to ∼200 bp sequences found in the coding regions of the roX1 and roX2 genes.
[ "24,25" ]
The first DCC binding sites characterized in this way correspond to ∼200 bp sequences found in the coding regions of the roX1 and roX2 genes.
true
true
true
true
true
840
2
INTRODUCTION
1
25
[ "B24", "B25", "B25", "B25", "B26" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
A prominent feature in these sequences is an abundance of GA sequences, and mutation of GAGAG tracts significantly reduced the recruiting power of the roX2 element (25).
[ "24", "25", "25", "25", "26" ]
169
5,041
1
false
A prominent feature in these sequences is an abundance of GA sequences, and mutation of GAGAG tracts significantly reduced the recruiting power of the roX2 element.
[ "25" ]
A prominent feature in these sequences is an abundance of GA sequences, and mutation of GAGAG tracts significantly reduced the recruiting power of the roX2 element.
true
true
true
true
true
840
2
INTRODUCTION
1
25
[ "B24", "B25", "B25", "B25", "B26" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
Unfortunately, bioinformatic efforts failed to detect related DCC targeting sequences on the X chromosome (25) and the third binding site characterized in some detail lacked GAGAG sequences altogether (26).
[ "24", "25", "25", "25", "26" ]
206
5,042
1
false
Unfortunately, bioinformatic efforts failed to detect related DCC targeting sequences on the X chromosome and the third binding site characterized in some detail lacked GAGAG sequences altogether.
[ "25", "26" ]
Unfortunately, bioinformatic efforts failed to detect related DCC targeting sequences on the X chromosome and the third binding site characterized in some detail lacked GAGAG sequences altogether.
true
true
true
true
true
840
2
INTRODUCTION
1
24
[ "B24", "B25", "B25", "B25", "B26" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
Primary DCC targeting elements thus differ in DNA sequence and may therefore belong to different classes.
[ "24", "25", "25", "25", "26" ]
105
5,043
0
false
Primary DCC targeting elements thus differ in DNA sequence and may therefore belong to different classes.
[]
Primary DCC targeting elements thus differ in DNA sequence and may therefore belong to different classes.
true
true
true
true
true
840
3
INTRODUCTION
1
26–30
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
According to the currently accepted model, many targeting elements of varying affinity are spread across the X chromosome.
[ "26–30", "29", "29", "29" ]
122
5,044
0
false
According to the currently accepted model, many targeting elements of varying affinity are spread across the X chromosome.
[]
According to the currently accepted model, many targeting elements of varying affinity are spread across the X chromosome.
true
true
true
true
true
841
3
INTRODUCTION
1
26–30
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
Strong targeting elements are able to autonomously recruit the DCC to an ectopic integration site on an autosome even at reduced concentrations of MSL proteins.
[ "26–30", "29", "29", "29" ]
160
5,045
0
false
Strong targeting elements are able to autonomously recruit the DCC to an ectopic integration site on an autosome even at reduced concentrations of MSL proteins.
[]
Strong targeting elements are able to autonomously recruit the DCC to an ectopic integration site on an autosome even at reduced concentrations of MSL proteins.
true
true
true
true
true
841
3
INTRODUCTION
1
26–30
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
In contrast, weaker sites are only bound in the X chromosomal context, presumably because the density of targeting elements leads to an increased local DCC concentration (26–30).
[ "26–30", "29", "29", "29" ]
178
5,046
1
false
In contrast, weaker sites are only bound in the X chromosomal context, presumably because the density of targeting elements leads to an increased local DCC concentration.
[ "26–30" ]
In contrast, weaker sites are only bound in the X chromosomal context, presumably because the density of targeting elements leads to an increased local DCC concentration.
true
true
true
true
true
841
3
INTRODUCTION
1
29
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
This model was substantiated by the analysis of 11 X-chromosomal fragments of varying affinity for DCC isolated by ChIP (29).
[ "26–30", "29", "29", "29" ]
125
5,047
1
false
This model was substantiated by the analysis of 11 X-chromosomal fragments of varying affinity for DCC isolated by ChIP.
[ "29" ]
This model was substantiated by the analysis of 11 X-chromosomal fragments of varying affinity for DCC isolated by ChIP.
true
true
true
true
true
841
3
INTRODUCTION
1
29
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
An attempt to identify sequence motifs responsible for DCC recruitment from a subset of these ‘DCC Binding Fragments’ (DBFs) with high- and moderate-affinity highlighted a number of clustered motifs (29).
[ "26–30", "29", "29", "29" ]
204
5,048
1
false
An attempt to identify sequence motifs responsible for DCC recruitment from a subset of these ‘DCC Binding Fragments’ (DBFs) with high- and moderate-affinity highlighted a number of clustered motifs.
[ "29" ]
An attempt to identify sequence motifs responsible for DCC recruitment from a subset of these ‘DCC Binding Fragments’ (DBFs) with high- and moderate-affinity highlighted a number of clustered motifs.
true
true
true
true
true
841
3
INTRODUCTION
1
26–30
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
Accordingly, a DCC binding site may be composed of clusters of variable combinations of several degenerate sequence motifs.
[ "26–30", "29", "29", "29" ]
123
5,049
0
false
Accordingly, a DCC binding site may be composed of clusters of variable combinations of several degenerate sequence motifs.
[]
Accordingly, a DCC binding site may be composed of clusters of variable combinations of several degenerate sequence motifs.
true
true
true
true
true
841
3
INTRODUCTION
1
29
[ "B26 B27 B28 B29 B30", "B29", "B29", "B29" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
However, perhaps due to the relatively large size of the fragments analysed, it was not possible to predict further DCC binding based on the clustering of these motifs (29).
[ "26–30", "29", "29", "29" ]
173
5,050
1
false
However, perhaps due to the relatively large size of the fragments analysed, it was not possible to predict further DCC binding based on the clustering of these motifs.
[ "29" ]
However, perhaps due to the relatively large size of the fragments analysed, it was not possible to predict further DCC binding based on the clustering of these motifs.
true
true
true
true
true
841
4
INTRODUCTION
1
21
[ "B21", "B22", "B24", "B26", "B29", "B29" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
Thus, several previous studies indicate that the DNA sequences comprising high affinity DCC binding sites are diverse, possess varying affinities for the DCC, and can be dispersed over several kb (21,22,24,26,29).
[ "21", "22", "24", "26", "29", "29" ]
213
5,051
0
false
Thus, several previous studies indicate that the DNA sequences comprising high affinity DCC binding sites are diverse, possess varying affinities for the DCC, and can be dispersed over several kb.
[ "21,22,24,26,29" ]
Thus, several previous studies indicate that the DNA sequences comprising high affinity DCC binding sites are diverse, possess varying affinities for the DCC, and can be dispersed over several kb.
true
true
true
true
true
842
4
INTRODUCTION
1
21
[ "B21", "B22", "B24", "B26", "B29", "B29" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
Given the degeneracy of the DNA motifs seen in association with DCC interaction, one has to assume that the contributions of individual elements to overall DCC targeting may be small and hence difficult to document by established methodology.
[ "21", "22", "24", "26", "29", "29" ]
242
5,052
0
false
Given the degeneracy of the DNA motifs seen in association with DCC interaction, one has to assume that the contributions of individual elements to overall DCC targeting may be small and hence difficult to document by established methodology.
[]
Given the degeneracy of the DNA motifs seen in association with DCC interaction, one has to assume that the contributions of individual elements to overall DCC targeting may be small and hence difficult to document by established methodology.
true
true
true
true
true
842
4
INTRODUCTION
1
21
[ "B21", "B22", "B24", "B26", "B29", "B29" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
In order to monitor the effect of weak targeting determinants, we developed a sensitive transfection-based ‘one-hybrid’ assay that amplifies weak DNA interaction events into a strong transcriptional read-out.
[ "21", "22", "24", "26", "29", "29" ]
208
5,053
0
false
In order to monitor the effect of weak targeting determinants, we developed a sensitive transfection-based ‘one-hybrid’ assay that amplifies weak DNA interaction events into a strong transcriptional read-out.
[]
In order to monitor the effect of weak targeting determinants, we developed a sensitive transfection-based ‘one-hybrid’ assay that amplifies weak DNA interaction events into a strong transcriptional read-out.
true
true
true
true
true
842
4
INTRODUCTION
1
21
[ "B21", "B22", "B24", "B26", "B29", "B29" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
The assay allows rapid identification of sequence elements able to recruit the MSL2 protein.
[ "21", "22", "24", "26", "29", "29" ]
92
5,054
0
false
The assay allows rapid identification of sequence elements able to recruit the MSL2 protein.
[]
The assay allows rapid identification of sequence elements able to recruit the MSL2 protein.
true
true
true
true
true
842
4
INTRODUCTION
1
29
[ "B21", "B22", "B24", "B26", "B29", "B29" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
We applied the assay to localize the DCC binding determinants within several previously described high affinity DBFs (29).
[ "21", "22", "24", "26", "29", "29" ]
122
5,055
1
false
We applied the assay to localize the DCC binding determinants within several previously described high affinity DBFs.
[ "29" ]
We applied the assay to localize the DCC binding determinants within several previously described high affinity DBFs.
true
true
true
true
true
842
4
INTRODUCTION
1
21
[ "B21", "B22", "B24", "B26", "B29", "B29" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
We describe several sequence motifs that contribute to MSL binding and show that high affinity MSL binding sites can be generated by oligomerization of weaker elements.
[ "21", "22", "24", "26", "29", "29" ]
168
5,056
0
false
We describe several sequence motifs that contribute to MSL binding and show that high affinity MSL binding sites can be generated by oligomerization of weaker elements.
[]
We describe several sequence motifs that contribute to MSL binding and show that high affinity MSL binding sites can be generated by oligomerization of weaker elements.
true
true
true
true
true
842
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
How the DCC of Drosophila recognizes the X chromosome for selective interaction is an unsolved question.
null
104
5,057
0
false
null
null
How the DCC of Drosophila recognizes the X chromosome for selective interaction is an unsolved question.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Although there is ample evidence that DNA sequences are involved, defining consensus sequence elements that may serve as binding sites for DCC components has been difficult.
null
173
5,058
0
false
null
null
Although there is ample evidence that DNA sequences are involved, defining consensus sequence elements that may serve as binding sites for DCC components has been difficult.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
The available evidence points to the existence of different sequence motifs, clustering in regions covering several kb, which form the highest affinity binding sites.
null
166
5,059
0
false
null
null
The available evidence points to the existence of different sequence motifs, clustering in regions covering several kb, which form the highest affinity binding sites.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Such a definition necessitates testing numerous candidate binding sites and extensive mutagenesis.
null
98
5,060
0
false
null
null
Such a definition necessitates testing numerous candidate binding sites and extensive mutagenesis.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
So far, the established method to evaluate X chromosomal sequences for DCC recruitment is time consuming since it involves generating stable fly lines containing candidate sequences integrated into an autosome.
null
210
5,061
0
false
null
null
So far, the established method to evaluate X chromosomal sequences for DCC recruitment is time consuming since it involves generating stable fly lines containing candidate sequences integrated into an autosome.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
The ‘one-hybrid’ strategy we introduced abbreviates this process dramatically.
null
78
5,062
0
false
null
null
The ‘one-hybrid’ strategy we introduced abbreviates this process dramatically.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Fusing the VP16 transactivation domain to MSL2 leads to a robust activation of a reporter gene provided that MSL2 is targeted to the candidate DNA upstream of a minimal promoter.
null
178
5,063
0
false
null
null
Fusing the VP16 transactivation domain to MSL2 leads to a robust activation of a reporter gene provided that MSL2 is targeted to the candidate DNA upstream of a minimal promoter.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
This strategy has several important features.
null
45
5,064
0
false
null
null
This strategy has several important features.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
First, the assay solely measures chromosome binding of MSL2 without constraints imposed by a requirement for normal function in dosage compensation.
null
148
5,065
0
false
null
null
First, the assay solely measures chromosome binding of MSL2 without constraints imposed by a requirement for normal function in dosage compensation.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
This allows mutating MSL2 regardless of potential consequences on functions other than recruitment.
null
99
5,066
0
false
null
null
This allows mutating MSL2 regardless of potential consequences on functions other than recruitment.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Second, for the assay to work it does not matter whether MSL2 binds the chromosome directly or indirectly via an adaptor molecule or even the entire DCC.
null
153
5,067
0
false
null
null
Second, for the assay to work it does not matter whether MSL2 binds the chromosome directly or indirectly via an adaptor molecule or even the entire DCC.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Third, the assay appears to be more sensitive for the identification of minimal targeting determinants than the polytene chromosome recruitment assay.
null
150
5,068
0
false
null
null
Third, the assay appears to be more sensitive for the identification of minimal targeting determinants than the polytene chromosome recruitment assay.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
The DBF12-L15 fragment would have been missed in the chromosome recruitment assay because its affinity for MSL2 is too weak if present as a monomer.
null
148
5,069
0
false
null
null
The DBF12-L15 fragment would have been missed in the chromosome recruitment assay because its affinity for MSL2 is too weak if present as a monomer.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
The element is nonetheless essential for DCC binding.
null
53
5,070
0
false
null
null
The element is nonetheless essential for DCC binding.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
However, the dramatic increase in MSL2-responsiveness upon trimerization led to uncovering its autonomous recruitment potential in flies.
null
137
5,071
0
false
null
null
However, the dramatic increase in MSL2-responsiveness upon trimerization led to uncovering its autonomous recruitment potential in flies.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
This enhanced sensitivity may be due to the fact that MSL2 is overexpressed in SL2 cells and hence present in artificially high concentrations that allow recognition of weak elements.
null
183
5,072
0
false
null
null
This enhanced sensitivity may be due to the fact that MSL2 is overexpressed in SL2 cells and hence present in artificially high concentrations that allow recognition of weak elements.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Native high affinity sites may be composites of several weak elements (see below) that individually are unable to attract DCC autonomously, but which can be detected in the one-hybrid assay.
null
190
5,073
0
false
null
null
Native high affinity sites may be composites of several weak elements (see below) that individually are unable to attract DCC autonomously, but which can be detected in the one-hybrid assay.
true
true
true
true
true
843
0
DISCUSSION
0
null
null
17,483,514
pmid-16204180|pmid-16204179|pmid-16507155|pmid-15165886|pmid-16515717|pmid-16821137|pmid-16285873|pmid-17173057|pmid-17173057|pmid-8288132|pmid-10882077|pmid-10594033|pmid-10831604|pmid-16307450|pmid-17035293|pmid-16543150|pmid-7781064|pmid-9409833|pmid-9736618|pmid-11013222|pmid-11867536
Finally, the assay may be adapted to a high-throughput format, which should allow screening many DNA sequences in parallel.
null
123
5,074
0
false
null
null
Finally, the assay may be adapted to a high-throughput format, which should allow screening many DNA sequences in parallel.
true
true
true
true
true
843
1
DISCUSSION
1
22
[ "B22", "B25", "B29", "B21", "B22", "B8", "B42", "B8" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
Notably, the DNA sequence elements identified as targeting determinants resemble those found earlier with the more established assay (see below (22,25,29)).
[ "22", "25", "29", "21", "22", "8", "42", "8" ]
156
5,075
0
false
Notably, the DNA sequence elements identified as targeting determinants resemble those found earlier with the more established assay ).
[ "see below (22,25,29" ]
Notably, the DNA sequence elements identified as targeting determinants resemble those found earlier with the more established assay ).
true
true
true
true
true
844
1
DISCUSSION
1
22
[ "B22", "B25", "B29", "B21", "B22", "B8", "B42", "B8" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
However, at this point we cannot exclude that the assay only detects a subset of DCC binding sites with special characteristics.
[ "22", "25", "29", "21", "22", "8", "42", "8" ]
128
5,076
0
false
However, at this point we cannot exclude that the assay only detects a subset of DCC binding sites with special characteristics.
[]
However, at this point we cannot exclude that the assay only detects a subset of DCC binding sites with special characteristics.
true
true
true
true
true
844
1
DISCUSSION
1
22
[ "B22", "B25", "B29", "B21", "B22", "B8", "B42", "B8" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
For example, all the core sequences identified to recruit MSL2 in this analysis lie in non-coding regions of the genome, whereas the majority of DCC binding is seen in coding regions (21,22).
[ "22", "25", "29", "21", "22", "8", "42", "8" ]
191
5,077
0
false
For example, all the core sequences identified to recruit MSL2 in this analysis lie in non-coding regions of the genome, whereas the majority of DCC binding is seen in coding regions.
[ "21,22" ]
For example, all the core sequences identified to recruit MSL2 in this analysis lie in non-coding regions of the genome, whereas the majority of DCC binding is seen in coding regions.
true
true
true
true
true
844
1
DISCUSSION
1
8
[ "B22", "B25", "B29", "B21", "B22", "B8", "B42", "B8" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
According to a recent model (8), the DCC may interact with chromosomes in two (or more) distinct binding modes: a primary mode, determined largely by DNA sequences, and a secondary mode employing transcription-associated epigenetic features.
[ "22", "25", "29", "21", "22", "8", "42", "8" ]
241
5,078
1
false
According to a recent model, the DCC may interact with chromosomes in two (or more) distinct binding modes: a primary mode, determined largely by DNA sequences, and a secondary mode employing transcription-associated epigenetic features.
[ "8" ]
According to a recent model, the DCC may interact with chromosomes in two (or more) distinct binding modes: a primary mode, determined largely by DNA sequences, and a secondary mode employing transcription-associated epigenetic features.
true
true
true
true
true
844
1
DISCUSSION
1
42
[ "B22", "B25", "B29", "B21", "B22", "B8", "B42", "B8" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
A similar model has recently been proposed for dosage compensation in C. elegans (42).
[ "22", "25", "29", "21", "22", "8", "42", "8" ]
86
5,079
1
false
A similar model has recently been proposed for dosage compensation in C. elegans.
[ "42" ]
A similar model has recently been proposed for dosage compensation in C. elegans.
true
true
true
true
true
844
1
DISCUSSION
1
8
[ "B22", "B25", "B29", "B21", "B22", "B8", "B42", "B8" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11445559|pmid-16547173|pmid-16547172|pmid-16547173|pmid-16547172|pmid-16547172|pmid-12718883|pmid-16462942|pmid-16547173|pmid-16547172|pmid-17173057|NA|pmid-17173057
Accordingly, distribution of the DCC over the X chromosome may involve primary recruitment to a subset of sites (possibly including those identified in this analysis) from which DCC is distributed to the majority of secondary sites (8).
[ "22", "25", "29", "21", "22", "8", "42", "8" ]
236
5,080
1
false
Accordingly, distribution of the DCC over the X chromosome may involve primary recruitment to a subset of sites (possibly including those identified in this analysis) from which DCC is distributed to the majority of secondary sites.
[ "8" ]
Accordingly, distribution of the DCC over the X chromosome may involve primary recruitment to a subset of sites (possibly including those identified in this analysis) from which DCC is distributed to the majority of secondary sites.
true
true
true
true
true
844
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
The one-hybrid assay allowed the fast mapping of minimal MSL2 targeting elements within larger DBFs identified by conventional means.
[ "25", "29" ]
133
5,081
0
false
The one-hybrid assay allowed the fast mapping of minimal MSL2 targeting elements within larger DBFs identified by conventional means.
[]
The one-hybrid assay allowed the fast mapping of minimal MSL2 targeting elements within larger DBFs identified by conventional means.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
These are the smallest known binding sites for the DCC.
[ "25", "29" ]
55
5,082
0
false
These are the smallest known binding sites for the DCC.
[]
These are the smallest known binding sites for the DCC.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
Deleting sequences from the DBFs in our quest for minimal elements we noticed the existence of ‘accessory’ elements, which by themselves are not sufficient to recruit the DCC in the transfection assay, but in the vicinity of a ‘core’ element contribute to the overall affinity.
[ "25", "29" ]
277
5,083
0
false
Deleting sequences from the DBFs in our quest for minimal elements we noticed the existence of ‘accessory’ elements, which by themselves are not sufficient to recruit the DCC in the transfection assay, but in the vicinity of a ‘core’ element contribute to the overall affinity.
[]
Deleting sequences from the DBFs in our quest for minimal elements we noticed the existence of ‘accessory’ elements, which by themselves are not sufficient to recruit the DCC in the transfection assay, but in the vicinity of a ‘core’ element contribute to the overall affinity.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
One such accessory motif consists of short CA dinucleotide repeats.
[ "25", "29" ]
67
5,084
0
false
One such accessory motif consists of short CA dinucleotide repeats.
[]
One such accessory motif consists of short CA dinucleotide repeats.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
The core elements appear purine-rich on one strand, and although purine-pyrimidine transversion affected activity of one nucleotide position, it could not account for all observed changes in activity, nor was the length of purine tract required for activity consistent between the different clones.
[ "25", "29" ]
298
5,085
0
false
The core elements appear purine-rich on one strand, and although purine-pyrimidine transversion affected activity of one nucleotide position, it could not account for all observed changes in activity, nor was the length of purine tract required for activity consistent between the different clones.
[]
The core elements appear purine-rich on one strand, and although purine-pyrimidine transversion affected activity of one nucleotide position, it could not account for all observed changes in activity, nor was the length of purine tract required for activity consistent between the different clones.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
The results therefore suggested that despite a general tolerance of mutation, some nucleotide positions within the core may be more important than others.
[ "25", "29" ]
154
5,086
0
false
The results therefore suggested that despite a general tolerance of mutation, some nucleotide positions within the core may be more important than others.
[]
The results therefore suggested that despite a general tolerance of mutation, some nucleotide positions within the core may be more important than others.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
However, the only sequence motifs shared between the three shortest elements isolated are AGA, GAG and AAA, and attempts to build longer, more flexible motifs, did not produce convincing results.
[ "25", "29" ]
195
5,087
0
false
However, the only sequence motifs shared between the three shortest elements isolated are AGA, GAG and AAA, and attempts to build longer, more flexible motifs, did not produce convincing results.
[]
However, the only sequence motifs shared between the three shortest elements isolated are AGA, GAG and AAA, and attempts to build longer, more flexible motifs, did not produce convincing results.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
The importance of GA-rich sequences for DCC recruitment has already been established for the roX loci (25), and they are also common in the DBF clones (29).
[ "25", "29" ]
156
5,088
1
false
The importance of GA-rich sequences for DCC recruitment has already been established for the roX loci, and they are also common in the DBF clones.
[ "25", "29" ]
The importance of GA-rich sequences for DCC recruitment has already been established for the roX loci, and they are also common in the DBF clones.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
However, the removal of the GAG motif from the shortest clones isolated from DBF9 (clones DBF9-L13 and -L14) does not lead to complete loss of MSL2 recruitment, confirming that not even this is an essential motif.
[ "25", "29" ]
213
5,089
0
false
However, the removal of the GAG motif from the shortest clones isolated from DBF9 (clones DBF9-L13 and -L14) does not lead to complete loss of MSL2 recruitment, confirming that not even this is an essential motif.
[]
However, the removal of the GAG motif from the shortest clones isolated from DBF9 (clones DBF9-L13 and -L14) does not lead to complete loss of MSL2 recruitment, confirming that not even this is an essential motif.
true
true
true
true
true
845
2
DISCUSSION
1
25
[ "B25", "B29" ]
17,483,514
pmid-11331589|pmid-12718883|pmid-12718883|pmid-12718883|pmid-15043812|pmid-12718883|pmid-16462942
On the other hand, trimerization of an element that essentially only consists of GA repeats can recruit MSL2 in our assay.
[ "25", "29" ]
122
5,090
0
false
On the other hand, trimerization of an element that essentially only consists of GA repeats can recruit MSL2 in our assay.
[]
On the other hand, trimerization of an element that essentially only consists of GA repeats can recruit MSL2 in our assay.
true
true
true
true
true
845
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
Together, these observations strengthen the earlier hypothesis that high affinity DBFs are composites of several distinct sequence motifs with variable DCC recruitment potential that synergise to generate a high affinity site.
[ "36", "43", "44", "42" ]
226
5,091
0
false
Together, these observations strengthen the earlier hypothesis that high affinity DBFs are composites of several distinct sequence motifs with variable DCC recruitment potential that synergise to generate a high affinity site.
[]
Together, these observations strengthen the earlier hypothesis that high affinity DBFs are composites of several distinct sequence motifs with variable DCC recruitment potential that synergise to generate a high affinity site.
true
true
true
true
true
846
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
These motifs may be dispersed, but cluster to form high affinity DBFs.
[ "36", "43", "44", "42" ]
70
5,092
0
false
These motifs may be dispersed, but cluster to form high affinity DBFs.
[]
These motifs may be dispersed, but cluster to form high affinity DBFs.
true
true
true
true
true
846
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
However, diversity in sequence appears not to be a fundamental requirement since high affinity sites can also be generated from homotypic elements by oligomerization, as shown here for two examples and as was also previously observed for a larger element (36).
[ "36", "43", "44", "42" ]
260
5,093
1
false
However, diversity in sequence appears not to be a fundamental requirement since high affinity sites can also be generated from homotypic elements by oligomerization, as shown here for two examples and as was also previously observed for a larger element.
[ "36" ]
However, diversity in sequence appears not to be a fundamental requirement since high affinity sites can also be generated from homotypic elements by oligomerization, as shown here for two examples and as was also previously observed for a larger element.
true
true
true
true
true
846
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
The relative tolerance towards point mutations emphasizes that these elements are degenerate.
[ "36", "43", "44", "42" ]
93
5,094
0
false
The relative tolerance towards point mutations emphasizes that these elements are degenerate.
[]
The relative tolerance towards point mutations emphasizes that these elements are degenerate.
true
true
true
true
true
846
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
The binding specificity of the DCC therefore seems surprisingly plastic, which may explain the failure of genome-wide binding analyses to define a single consensus.
[ "36", "43", "44", "42" ]
164
5,095
0
false
The binding specificity of the DCC therefore seems surprisingly plastic, which may explain the failure of genome-wide binding analyses to define a single consensus.
[]
The binding specificity of the DCC therefore seems surprisingly plastic, which may explain the failure of genome-wide binding analyses to define a single consensus.
true
true
true
true
true
846
3
DISCUSSION
1
43
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
The observed degeneracy implies that each single targeting determinant has a relatively low affinity for DCC, and we have to assume that the sum of many weak interactions effectively generate high-affinity DBFs (43).
[ "36", "43", "44", "42" ]
216
5,096
1
false
The observed degeneracy implies that each single targeting determinant has a relatively low affinity for DCC, and we have to assume that the sum of many weak interactions effectively generate high-affinity DBFs.
[ "43" ]
The observed degeneracy implies that each single targeting determinant has a relatively low affinity for DCC, and we have to assume that the sum of many weak interactions effectively generate high-affinity DBFs.
true
true
true
true
true
846
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
Ultimately, the future identification of a greater number of targeting elements should allow a better definition of the motifs recognized by the DCC.
[ "36", "43", "44", "42" ]
149
5,097
0
false
Ultimately, the future identification of a greater number of targeting elements should allow a better definition of the motifs recognized by the DCC.
[]
Ultimately, the future identification of a greater number of targeting elements should allow a better definition of the motifs recognized by the DCC.
true
true
true
true
true
846
3
DISCUSSION
1
36
[ "B36", "B43", "B42", "B44" ]
17,483,514
pmid-15043812|pmid-14579126|pmid-15502872|pmid-16462942|pmid-15943970|pmid-16462942|pmid-16462942|pmid-16462942|pmid-16117636|pmid-3360135|NA|pmid-17122774
Our conclusions are in broad agreement with recent results from C. elegans, where isolated or clustered motifs can render a high affinity DBF, but are not sufficient to explain all observed DCC binding (44,42).
[ "36", "43", "44", "42" ]
210
5,098
0
false
Our conclusions are in broad agreement with recent results from C. elegans, where isolated or clustered motifs can render a high affinity DBF, but are not sufficient to explain all observed DCC binding.
[ "44,42" ]
Our conclusions are in broad agreement with recent results from C. elegans, where isolated or clustered motifs can render a high affinity DBF, but are not sufficient to explain all observed DCC binding.
true
true
true
true
true
846
4
DISCUSSION
1
24–26
[ "B24 B25 B26", "B26", "B45", "B46", "B47" ]
17,483,514
pmid-16547173|pmid-16547172|pmid-11331589|pmid-15043812|pmid-16462942|pmid-16462942|pmid-11331589|pmid-12718883|pmid-15043812|pmid-15043812|pmid-15961632|pmid-8107823|pmid-11583616
Emulating previous studies (24–26), DH sites were found in all of the five high affinity DCC binding sites studied in this analysis.
[ "24–26", "26", "45", "46", "47" ]
132
5,099
1
false
Emulating previous studies, DH sites were found in all of the five high affinity DCC binding sites studied in this analysis.
[ "24–26" ]
Emulating previous studies, DH sites were found in all of the five high affinity DCC binding sites studied in this analysis.
true
true
true
true
true
847