Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
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3
INTRODUCTION
1
24
[ "B24", "B25", "B24", "B25", "B26", "B27 B28 B29", "B28", "B12" ]
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
Figure 1.Characterization of the vRNPs of hRSV in live cells using molecular beacon imaging.
[ "24", "25", "24", "25", "26", "27–29", "28", "12" ]
92
5,200
0
false
Figure 1.Characterization of the vRNPs of hRSV in live cells using molecular beacon imaging.
[]
Figure 1.Characterization of the vRNPs of hRSV in live cells using molecular beacon imaging.
true
true
true
true
true
867
3
INTRODUCTION
1
24
[ "B24", "B25", "B24", "B25", "B26", "B27 B28 B29", "B28", "B12" ]
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
A schematic showing the genomic organization of hRSV with black ellipses indicating the sequence locations where the molecular beacon probe was targeted.
[ "24", "25", "24", "25", "26", "27–29", "28", "12" ]
153
5,201
0
false
A schematic showing the genomic organization of hRSV with black ellipses indicating the sequence locations where the molecular beacon probe was targeted.
[]
A schematic showing the genomic organization of hRSV with black ellipses indicating the sequence locations where the molecular beacon probe was targeted.
true
true
true
true
true
867
3
INTRODUCTION
1
24
[ "B24", "B25", "B24", "B25", "B26", "B27 B28 B29", "B28", "B12" ]
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
(B) Image of non-infected (NI) and 2-day post-infection (IN) Vero cells, 30 min after MBs targeting the genomic vRNA of hRSV were delivered into them; infected cells exhibit highly localized signal in both inclusion bodies (aggregates of vRNA) and groups of filamentous vRNPs.
[ "24", "25", "24", "25", "26", "27–29", "28", "12" ]
276
5,202
0
false
(B) Image of non-infected (NI) and 2-day post-infection (IN) Vero cells, 30 min after MBs targeting the genomic vRNA of hRSV were delivered into them; infected cells exhibit highly localized signal in both inclusion bodies (aggregates of vRNA) and groups of filamentous vRNPs.
[]
(B) Image of non-infected (NI) and 2-day post-infection (IN) Vero cells, 30 min after MBs targeting the genomic vRNA of hRSV were delivered into them; infected cells exhibit highly localized signal in both inclusion bodies (aggregates of vRNA) and groups of filamentous vRNPs.
false
false
true
true
false
867
3
INTRODUCTION
1
24
[ "B24", "B25", "B24", "B25", "B26", "B27 B28 B29", "B28", "B12" ]
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
(C) PCR results from non-infected (NI) and infected (IN) cells 2-day post-infection; no product was formed for the NI case, while the IN case showed a strong PCR product at 940 bp.
[ "24", "25", "24", "25", "26", "27–29", "28", "12" ]
180
5,203
0
false
(C) PCR results from non-infected (NI) and infected (IN) cells 2-day post-infection; no product was formed for the NI case, while the IN case showed a strong PCR product at 940 bp.
[]
(C) PCR results from non-infected (NI) and infected (IN) cells 2-day post-infection; no product was formed for the NI case, while the IN case showed a strong PCR product at 940 bp.
false
false
true
true
false
867
3
INTRODUCTION
1
24
[ "B24", "B25", "B24", "B25", "B26", "B27 B28 B29", "B28", "B12" ]
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
(D) Results from live-cell delivery and hybridization of Quasar570-labeled MB to vRNA in infected cells, together with fixation and antibody staining (Alexa488) for hRSV F protein, indicating that molecular beacons could hybridize to vRNPs when they are being packaged for egress.
[ "24", "25", "24", "25", "26", "27–29", "28", "12" ]
280
5,204
0
false
(D) Results from live-cell delivery and hybridization of Quasar570-labeled MB to vRNA in infected cells, together with fixation and antibody staining (Alexa488) for hRSV F protein, indicating that molecular beacons could hybridize to vRNPs when they are being packaged for egress.
[]
(D) Results from live-cell delivery and hybridization of Quasar570-labeled MB to vRNA in infected cells, together with fixation and antibody staining for hRSV F protein, indicating that molecular beacons could hybridize to vRNPs when they are being packaged for egress.
false
false
true
true
false
867
4
INTRODUCTION
0
null
null
17,485,480
null
Characterization of the vRNPs of hRSV in live cells using molecular beacon imaging.
null
83
5,205
0
false
null
null
Characterization of the vRNPs of hRSV in live cells using molecular beacon imaging.
true
true
true
true
true
868
4
INTRODUCTION
0
null
null
17,485,480
null
A schematic showing the genomic organization of hRSV with black ellipses indicating the sequence locations where the molecular beacon probe was targeted.
null
153
5,206
0
false
null
null
A schematic showing the genomic organization of hRSV with black ellipses indicating the sequence locations where the molecular beacon probe was targeted.
true
true
true
true
true
868
4
INTRODUCTION
0
null
null
17,485,480
null
(B) Image of non-infected (NI) and 2-day post-infection (IN) Vero cells, 30 min after MBs targeting the genomic vRNA of hRSV were delivered into them; infected cells exhibit highly localized signal in both inclusion bodies (aggregates of vRNA) and groups of filamentous vRNPs.
null
276
5,207
0
false
null
null
(B) Image of non-infected (NI) and 2-day post-infection (IN) Vero cells, 30 min after MBs targeting the genomic vRNA of hRSV were delivered into them; infected cells exhibit highly localized signal in both inclusion bodies (aggregates of vRNA) and groups of filamentous vRNPs.
false
false
true
true
false
868
4
INTRODUCTION
0
null
null
17,485,480
null
(C) PCR results from non-infected (NI) and infected (IN) cells 2-day post-infection; no product was formed for the NI case, while the IN case showed a strong PCR product at 940 bp.
null
180
5,208
0
false
null
null
(C) PCR results from non-infected (NI) and infected (IN) cells 2-day post-infection; no product was formed for the NI case, while the IN case showed a strong PCR product at 940 bp.
false
false
true
true
false
868
4
INTRODUCTION
0
null
null
17,485,480
null
(D) Results from live-cell delivery and hybridization of Quasar570-labeled MB to vRNA in infected cells, together with fixation and antibody staining (Alexa488) for hRSV F protein, indicating that molecular beacons could hybridize to vRNPs when they are being packaged for egress.
null
280
5,209
0
false
null
null
(D) Results from live-cell delivery and hybridization of Quasar570-labeled MB to vRNA in infected cells, together with fixation and antibody staining (Alexa488) for hRSV F protein, indicating that molecular beacons could hybridize to vRNPs when they are being packaged for egress.
false
false
true
true
false
868
0
DISCUSSION
1
34–36
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
In the previous section, evidence of the directed motion of the RSV filamentous vRNPs was presented.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
100
5,210
0
false
In the previous section, evidence of the directed motion of the RSV filamentous vRNPs was presented.
[]
In the previous section, evidence of the directed motion of the RSV filamentous vRNPs was presented.
true
true
true
true
true
869
0
DISCUSSION
1
34–36
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
This evidence, in conjunction with previous studies of RSV filaments (34–36,42–46), points to motor driven transport on the cytoskeleton as the likely driver of the observed migratory motion, and possibly the rotational motion.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
227
5,211
0
false
This evidence, in conjunction with previous studies of RSV filaments, points to motor driven transport on the cytoskeleton as the likely driver of the observed migratory motion, and possibly the rotational motion.
[ "34–36,42–46" ]
This evidence, in conjunction with previous studies of RSV filaments, points to motor driven transport on the cytoskeleton as the likely driver of the observed migratory motion, and possibly the rotational motion.
true
true
true
true
true
869
0
DISCUSSION
1
43
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
Previous studies of RSV have demonstrated that actin filaments (42,46) and their growth, mediated by profilin (43) and rhoA activation (44), are vital to virion egress.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
168
5,212
1
false
Previous studies of RSV have demonstrated that actin filaments and their growth, mediated by profilin and rhoA activation, are vital to virion egress.
[ "42,46", "43", "44" ]
Previous studies of RSV have demonstrated that actin filaments and their growth, mediated by profilin and rhoA activation, are vital to virion egress.
true
true
true
true
true
869
0
DISCUSSION
1
34–36
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
In many of those studies, infected cells were exposed to cytoskeletal depolymerizing agents.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
92
5,213
0
false
In many of those studies, infected cells were exposed to cytoskeletal depolymerizing agents.
[]
In many of those studies, infected cells were exposed to cytoskeletal depolymerizing agents.
true
true
true
true
true
869
0
DISCUSSION
1
34–36
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
The amount of free virion or viral RNA in the cell culture media, or changes in virion morphology, as measured via immunofluorescence imaging, were used to gauge the effect of these agents on virion egress.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
206
5,214
0
false
The amount of free virion or viral RNA in the cell culture media, or changes in virion morphology, as measured via immunofluorescence imaging, were used to gauge the effect of these agents on virion egress.
[]
The amount of free virion or viral RNA in the cell culture media, or changes in virion morphology, as measured via immunofluorescence imaging, were used to gauge the effect of these agents on virion egress.
true
true
true
true
true
869
0
DISCUSSION
1
34–36
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
In previous studies, actin depolymerization via cytochalasin D had the most adverse effects on virion egress, therefore making actin/myosin the most likely cytoskeletal/motor combination involved in the motion observed in our investigation.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
240
5,215
0
false
In previous studies, actin depolymerization via cytochalasin D had the most adverse effects on virion egress, therefore making actin/myosin the most likely cytoskeletal/motor combination involved in the motion observed in our investigation.
[]
In previous studies, actin depolymerization via cytochalasin D had the most adverse effects on virion egress, therefore making actin/myosin the most likely cytoskeletal/motor combination involved in the motion observed in our investigation.
true
true
true
true
true
869
0
DISCUSSION
1
39
[ "B34 B35 B36", "B42 B43 B44 B45 B46", "B42", "B46", "B43", "B44", "B39" ]
17,485,480
pmid-21016866|pmid-13483636|pmid-9576955|NA|pmid-4128827|pmid-4213002|pmid-7884920|pmid-4100527|pmid-10769073|pmid-7884920|pmid-13483636|pmid-9576955|pmid-11842256|pmid-12124448|pmid-12642099|pmid-9875324|pmid-12740026|pmid-15827147|pmid-15582654|pmid-9617766|pmid-9875324|pmid-9617766|pmid-12740026|pmid-15827147|pmid-1...
In addition, since assembly occurs at the lipid rafts and the rafts are linked to the actin network (39), depolymerzing them should effect the observed motion.
[ "34–36", "42–46", "42", "46", "43", "44", "39" ]
159
5,216
1
false
In addition, since assembly occurs at the lipid rafts and the rafts are linked to the actin network, depolymerzing them should effect the observed motion.
[ "39" ]
In addition, since assembly occurs at the lipid rafts and the rafts are linked to the actin network, depolymerzing them should effect the observed motion.
true
true
true
true
true
869
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
Live-cell MB hybridization, cell fixation and permeabilization, followed by immunostaining of myosin Va, and confocal microscopy imaging was performed in order to confirm the presence and possible role of myosin.
[ "47", "48", "38" ]
212
5,217
0
false
Live-cell MB hybridization, cell fixation and permeabilization, followed by immunostaining of myosin Va, and confocal microscopy imaging was performed in order to confirm the presence and possible role of myosin.
[]
Live-cell MB hybridization, cell fixation and permeabilization, followed by immunostaining of myosin Va, and confocal microscopy imaging was performed in order to confirm the presence and possible role of myosin.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
Myosin V has previously been implicated in trafficking messenger RNP (47), granules on the actin network near the plasma membrane (48), and RSV proteins to the apical membrane in polarized cells (38).
[ "47", "48", "38" ]
200
5,218
1
false
Myosin V has previously been implicated in trafficking messenger RNP, granules on the actin network near the plasma membrane, and RSV proteins to the apical membrane in polarized cells.
[ "47", "48", "38" ]
Myosin V has previously been implicated in trafficking messenger RNP, granules on the actin network near the plasma membrane, and RSV proteins to the apical membrane in polarized cells.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
The results from our simultaneous labeling experiments can be seen in Figure 5, where the blue represents myosin Va, the red represents vRNPs (hybridized MBs) and the purple represents their colocalization.
[ "47", "48", "38" ]
206
5,219
0
false
The results from our simultaneous labeling experiments can be seen in Figure 5, where the blue represents myosin Va, the red represents vRNPs (hybridized MBs) and the purple represents their colocalization.
[]
The results from our simultaneous labeling experiments can be seen in Figure 5, where the blue represents myosin Va, the red represents vRNPs (hybridized MBs) and the purple represents their colocalization.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
In Figure 5A, like Figure 2A, four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 μm from the glass surface are shown.
[ "47", "48", "38" ]
160
5,220
0
false
In Figure 5A, like Figure 2A, four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 ΞΌm from the glass surface are shown.
[]
In Figure 5A, like Figure 2A, four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 ΞΌm from the glass surface are shown.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
The 0 μm view represents all of the fluorescent signal from within the infected cells, displayed in two dimensions; the cross section is ∼12 μm thick from the coverglass to the tops of the viral filaments.
[ "47", "48", "38" ]
205
5,221
0
false
The 0 μm view represents all of the fluorescent signal from within the infected cells, displayed in two dimensions; the cross section is ∼12 μm thick from the coverglass to the tops of the viral filaments.
[]
The 0 μm view represents all of the fluorescent signal from within the infected cells, displayed in two dimensions; the cross section is ∼12 μm thick from the coverglass to the tops of the viral filaments.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
In Figure 5B, XZ and YZ cross-sectional views are shown, with crossed lines representing where they were taken in the XY plane.
[ "47", "48", "38" ]
127
5,222
0
false
In Figure 5B, XZ and YZ cross-sectional views are shown, with crossed lines representing where they were taken in the XY plane.
[]
In Figure 5B, XZ and YZ cross-sectional views are shown, with crossed lines representing where they were taken in the XY plane.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
From the data in Figure 5A and B, it can be seen that the filaments are largely distributed along the apical surface, and that they colocalize well with myosin Va (purple color).
[ "47", "48", "38" ]
178
5,223
0
false
From the data in Figure 5A and B, it can be seen that the filaments are largely distributed along the apical surface, and that they colocalize well with myosin Va (purple color).
[]
From the data in Figure 5A and B, it can be seen that the filaments are largely distributed along the apical surface, and that they colocalize well with myosin Va (purple color).
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
Throughout the volume of the cell monolayer, both myosin Va (blue), represented as small dots, and vRNPs (MB, red), predominately in cytoplasmic inclusions, do not colocalize and appear independent.
[ "47", "48", "38" ]
198
5,224
0
false
Throughout the volume of the cell monolayer, both myosin Va (blue), represented as small dots, and vRNPs (MB, red), predominately in cytoplasmic inclusions, do not colocalize and appear independent.
[]
Throughout the volume of the cell monolayer, both myosin Va (blue), represented as small dots, and vRNPs (MB, red), predominately in cytoplasmic inclusions, do not colocalize and appear independent.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
It is clear from this figure that the strongest colocalization occurs predominately in the filaments and near the surface of the monolayer, implicating myosin Va's role in the assembly and egress process.
[ "47", "48", "38" ]
204
5,225
0
false
It is clear from this figure that the strongest colocalization occurs predominately in the filaments and near the surface of the monolayer, implicating myosin Va's role in the assembly and egress process.
[]
It is clear from this figure that the strongest colocalization occurs predominately in the filaments and near the surface of the monolayer, implicating myosin Va's role in the assembly and egress process.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
Figure 5.Simultaneous imaging of vRNPs and Myosin Va. (A) Four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 μm from the glass surface are shown.
[ "47", "48", "38" ]
188
5,226
0
false
Figure 5.Simultaneous imaging of vRNPs and Myosin Va. (A) Four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 ΞΌm from the glass surface are shown.
[]
Figure 5.Simultaneous imaging of vRNPs and Myosin Va. (A) Four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 ΞΌm from the glass surface are shown.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
The cross section is 12 μm thick from the coverglass to the tops of the filaments.
[ "47", "48", "38" ]
82
5,227
0
false
The cross section is 12 ΞΌm thick from the coverglass to the tops of the filaments.
[]
The cross section is 12 ΞΌm thick from the coverglass to the tops of the filaments.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
Myosin Va is represented in blue, vRNPs in red and the colocalization in purple.
[ "47", "48", "38" ]
80
5,228
0
false
Myosin Va is represented in blue, vRNPs in red and the colocalization in purple.
[]
Myosin Va is represented in blue, vRNPs in red and the colocalization in purple.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
(B) XZ and YZ cross-sectional views.
[ "47", "48", "38" ]
36
5,229
0
false
(B) XZ and YZ cross-sectional views.
[]
(B) XZ and YZ cross-sectional views.
false
false
true
true
false
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
The filaments are largely distributed along the apical surface, and colocalize with myosin Va.
[ "47", "48", "38" ]
94
5,230
0
false
The filaments are largely distributed along the apical surface, and colocalize with myosin Va.
[]
The filaments are largely distributed along the apical surface, and colocalize with myosin Va.
true
true
true
true
true
870
1
DISCUSSION
1
47
[ "B47", "B48", "B38" ]
17,485,480
pmid-3182934|pmid-10064612|pmid-17052171|pmid-14630951
Throughout the volume of the cell monolayer, myosin Va, small dots and vRNPs, predominately in cytoplasmic inclusions, do not colocalize and appear independent.
[ "47", "48", "38" ]
160
5,231
0
false
Throughout the volume of the cell monolayer, myosin Va, small dots and vRNPs, predominately in cytoplasmic inclusions, do not colocalize and appear independent.
[]
Throughout the volume of the cell monolayer, myosin Va, small dots and vRNPs, predominately in cytoplasmic inclusions, do not colocalize and appear independent.
true
true
true
true
true
870
2
DISCUSSION
0
null
null
17,485,480
pmid-9630890|pmid-15084672|pmid-15377515|pmid-14583593|pmid-15809226|pmid-15084673|pmid-16378971|pmid-15942027|pmid-7691830|pmid-9653126|pmid-16483318|pmid-15454466|pmid-15205532
Simultaneous imaging of vRNPs and Myosin Va. (A) Four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 μm from the glass surface are shown.
null
179
5,232
0
false
null
null
Simultaneous imaging of vRNPs and Myosin Va. (A) Four images showing only the fluorescence information at distances greater than 9, 6, 3 and 0 μm from the glass surface are shown.
true
true
true
true
true
871
2
DISCUSSION
0
null
null
17,485,480
pmid-9630890|pmid-15084672|pmid-15377515|pmid-14583593|pmid-15809226|pmid-15084673|pmid-16378971|pmid-15942027|pmid-7691830|pmid-9653126|pmid-16483318|pmid-15454466|pmid-15205532
The cross section is 12 μm thick from the coverglass to the tops of the filaments.
null
82
5,233
0
false
null
null
The cross section is 12 μm thick from the coverglass to the tops of the filaments.
true
true
true
true
true
871
2
DISCUSSION
0
null
null
17,485,480
pmid-9630890|pmid-15084672|pmid-15377515|pmid-14583593|pmid-15809226|pmid-15084673|pmid-16378971|pmid-15942027|pmid-7691830|pmid-9653126|pmid-16483318|pmid-15454466|pmid-15205532
Myosin Va is represented in blue, vRNPs in red and the colocalization in purple.
null
80
5,234
0
false
null
null
Myosin Va is represented in blue, vRNPs in red and the colocalization in purple.
true
true
true
true
true
871
2
DISCUSSION
0
null
null
17,485,480
pmid-9630890|pmid-15084672|pmid-15377515|pmid-14583593|pmid-15809226|pmid-15084673|pmid-16378971|pmid-15942027|pmid-7691830|pmid-9653126|pmid-16483318|pmid-15454466|pmid-15205532
(B) XZ and YZ cross-sectional views.
null
36
5,235
0
false
null
null
(B) XZ and YZ cross-sectional views.
false
false
true
true
false
871
2
DISCUSSION
0
null
null
17,485,480
pmid-9630890|pmid-15084672|pmid-15377515|pmid-14583593|pmid-15809226|pmid-15084673|pmid-16378971|pmid-15942027|pmid-7691830|pmid-9653126|pmid-16483318|pmid-15454466|pmid-15205532
The filaments are largely distributed along the apical surface, and colocalize with myosin Va.
null
94
5,236
0
false
null
null
The filaments are largely distributed along the apical surface, and colocalize with myosin Va.
true
true
true
true
true
871
2
DISCUSSION
0
null
null
17,485,480
pmid-9630890|pmid-15084672|pmid-15377515|pmid-14583593|pmid-15809226|pmid-15084673|pmid-16378971|pmid-15942027|pmid-7691830|pmid-9653126|pmid-16483318|pmid-15454466|pmid-15205532
Throughout the volume of the cell monolayer, myosin Va, small dots and vRNPs, predominately in cytoplasmic inclusions, do not colocalize and appear independent.
null
160
5,237
0
false
null
null
Throughout the volume of the cell monolayer, myosin Va, small dots and vRNPs, predominately in cytoplasmic inclusions, do not colocalize and appear independent.
true
true
true
true
true
871
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
Vero cells 2-day PI and hybridized with RSV targeted MBs, were exposed to cytochalasin D, an actin depolymerising agent, in order to provide further evidence for the role of actin on the vRNP morphology and dynamics.
null
216
5,238
0
false
null
null
Vero cells 2-day PI and hybridized with RSV targeted MBs, were exposed to cytochalasin D, an actin depolymerising agent, in order to provide further evidence for the role of actin on the vRNP morphology and dynamics.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
We observed that many filamentous vRNPs exposed to cytochalasin D, tended to aggregate and form circular, inclusion-like structures of vRNPs within ∼1 min (Figure 6A and Supplementary Movie 6).
null
193
5,239
0
false
null
null
We observed that many filamentous vRNPs exposed to cytochalasin D, tended to aggregate and form circular, inclusion-like structures of vRNPs within ∼1 min (Figure 6A and Supplementary Movie 6).
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
Two minutes later, these circular aggregates tended to move from the surface of the cell into the cell cytoplasm.
null
113
5,240
0
false
null
null
Two minutes later, these circular aggregates tended to move from the surface of the cell into the cell cytoplasm.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
After 15 min, almost all of the vRNPs had separated from the cell surface and were concentrated in granules.
null
108
5,241
0
false
null
null
After 15 min, almost all of the vRNPs had separated from the cell surface and were concentrated in granules.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
In Figure 6B, a typical syncytia is shown; high concentrations of filamentous structures surround the syncytia, while a lower density of filaments and particles lie along the apical membrane.
null
191
5,242
0
false
null
null
In Figure 6B, a typical syncytia is shown; high concentrations of filamentous structures surround the syncytia, while a lower density of filaments and particles lie along the apical membrane.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
However, as shown in Figure 6C, a syncytia 15 min after cytochalasin D exposure composed of predominately circular granules.
null
124
5,243
0
false
null
null
However, as shown in Figure 6C, a syncytia 15 min after cytochalasin D exposure composed of predominately circular granules.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
This experiment helps confirm filamentous actin's role in governing vRNP morphology, and explain why virion egress is decreased when actin filaments are depolymerized.
null
167
5,244
0
false
null
null
This experiment helps confirm filamentous actin's role in governing vRNP morphology, and explain why virion egress is decreased when actin filaments are depolymerized.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
Figure 6.Actin's role in vRNP dynamics and morphology.
null
54
5,245
0
false
null
null
Figure 6.Actin's role in vRNP dynamics and morphology.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
(A) Seven images representing the time-lapse effects of cytochalasin D on the morphology and location of filamentous vRNPs.
null
123
5,246
0
false
null
null
(A) Seven images representing the time-lapse effects of cytochalasin D on the morphology and location of filamentous vRNPs.
false
false
true
true
false
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
Initially filamentous vRNPs tended to aggregate and form circular aggregates within 1 min of exposure to media containing cytochalasin D.
null
137
5,247
0
false
null
null
Initially filamentous vRNPs tended to aggregate and form circular aggregates within 1 min of exposure to media containing cytochalasin D.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
By 3 min, these circular aggregates, tended to move from the surface of the cell into the cell cytoplasm.
null
105
5,248
0
false
null
null
By 3 min, these circular aggregates, tended to move from the surface of the cell into the cell cytoplasm.
true
true
true
true
true
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
(B) Representative example of a typical syncytia, showing high concentrations of filamentous structures around the outside of the syncytia and a lower concentration along the apical surface.
null
190
5,249
0
false
null
null
(B) Representative example of a typical syncytia, showing high concentrations of filamentous structures around the outside of the syncytia and a lower concentration along the apical surface.
false
false
true
true
false
872
3
DISCUSSION
0
null
null
17,485,480
pmid-9671772|pmid-9037376|pmid-9671772|pmid-9037376|pmid-12771228|pmid-9547284|pmid-2726482|pmid-12669716|pmid-2726482|pmid-15084672
(C) Representative image of a syncytia after cytochalasin D exposure, composed of predominately circular granules.
null
114
5,250
0
false
null
null
(C) Representative image of a syncytia after cytochalasin D exposure, composed of predominately circular granules.
false
false
true
true
false
872
4
DISCUSSION
0
null
null
17,485,480
null
Actin's role in vRNP dynamics and morphology.
null
45
5,251
0
false
null
null
Actin's role in vRNP dynamics and morphology.
true
true
true
true
true
873
4
DISCUSSION
0
null
null
17,485,480
null
(A) Seven images representing the time-lapse effects of cytochalasin D on the morphology and location of filamentous vRNPs.
null
123
5,252
0
false
null
null
(A) Seven images representing the time-lapse effects of cytochalasin D on the morphology and location of filamentous vRNPs.
false
false
true
true
false
873
4
DISCUSSION
0
null
null
17,485,480
null
Initially filamentous vRNPs tended to aggregate and form circular aggregates within 1 min of exposure to media containing cytochalasin D.
null
137
5,253
0
false
null
null
Initially filamentous vRNPs tended to aggregate and form circular aggregates within 1 min of exposure to media containing cytochalasin D.
true
true
true
true
true
873
4
DISCUSSION
0
null
null
17,485,480
null
By 3 min, these circular aggregates, tended to move from the surface of the cell into the cell cytoplasm.
null
105
5,254
0
false
null
null
By 3 min, these circular aggregates, tended to move from the surface of the cell into the cell cytoplasm.
true
true
true
true
true
873
4
DISCUSSION
0
null
null
17,485,480
null
(B) Representative example of a typical syncytia, showing high concentrations of filamentous structures around the outside of the syncytia and a lower concentration along the apical surface.
null
190
5,255
0
false
null
null
(B) Representative example of a typical syncytia, showing high concentrations of filamentous structures around the outside of the syncytia and a lower concentration along the apical surface.
false
false
true
true
false
873
4
DISCUSSION
0
null
null
17,485,480
null
(C) Representative image of a syncytia after cytochalasin D exposure, composed of predominately circular granules.
null
114
5,256
0
false
null
null
(C) Representative image of a syncytia after cytochalasin D exposure, composed of predominately circular granules.
false
false
true
true
false
873
5
DISCUSSION
0
null
null
17,485,480
null
From the data presented in this investigation and from previous studies, we hypothesize that actin filaments and myosin-driven motion are the root cause of the motion observed, and such motion is mechanically separating the packaged vRNPs from the actin network, thus allowing them to leave the cell.
null
300
5,257
0
false
null
null
From the data presented in this investigation and from previous studies, we hypothesize that actin filaments and myosin-driven motion are the root cause of the motion observed, and such motion is mechanically separating the packaged vRNPs from the actin network, thus allowing them to leave the cell.
true
true
true
true
true
874
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
In order to further explain the observed dynamics of filamentous vRNPs in live cells, we propose a biophysical model.
[ "31", "49", "37", "31" ]
117
5,258
0
false
In order to further explain the observed dynamics of filamentous vRNPs in live cells, we propose a biophysical model.
[]
In order to further explain the observed dynamics of filamentous vRNPs in live cells, we propose a biophysical model.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
Presented in Figure 7A–D are sketches of the possible actin network/nucleocapsid interactions for all three types of motion discussed above.
[ "31", "49", "37", "31" ]
140
5,259
0
false
Presented in Figure 7A–D are sketches of the possible actin network/nucleocapsid interactions for all three types of motion discussed above.
[]
Presented in Figure 7A–D are sketches of the possible actin network/nucleocapsid interactions for all three types of motion discussed above.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
Figure 7A and B represents how vRNP rotation might be achieved.
[ "31", "49", "37", "31" ]
63
5,260
0
false
Figure 7A and B represents how vRNP rotation might be achieved.
[]
Figure 7A and B represents how vRNP rotation might be achieved.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
Myosin motors, attached to the vRNP and moving along the actin network, could cause the virion to flip up on one end (Figure 3A), given that actin filaments are quite flexible.
[ "31", "49", "37", "31" ]
176
5,261
0
false
Myosin motors, attached to the vRNP and moving along the actin network, could cause the virion to flip up on one end (Figure 3A), given that actin filaments are quite flexible.
[]
Myosin motors, attached to the vRNP and moving along the actin network, could cause the virion to flip up on one end, given that actin filaments are quite flexible.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
It is possible that this motion frees much of the virion from the plasma membrane, allowing for more rigorous rotation (Figure 3C) or migration (Figure 4A).
[ "31", "49", "37", "31" ]
156
5,262
0
false
It is possible that this motion frees much of the virion from the plasma membrane, allowing for more rigorous rotation (Figure 3C) or migration (Figure 4A).
[]
It is possible that this motion frees much of the virion from the plasma membrane, allowing for more rigorous rotation or migration (Figure 4A).
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
The rigorous rotation may cause the separation of daughter actin strands within the virion from mother strands at the Arp2/3 interface (31,49).
[ "31", "49", "37", "31" ]
143
5,263
0
false
The rigorous rotation may cause the separation of daughter actin strands within the virion from mother strands at the Arp2/3 interface.
[ "31,49" ]
The rigorous rotation may cause the separation of daughter actin strands within the virion from mother strands at the Arp2/3 interface.
true
true
true
true
true
875
6
DISCUSSION
1
37
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
The dissociation constant for Arp2/3 with the mother strand is ∼50 times higher than with the daughter strand, and thus the weakest part of the structure (37).
[ "31", "49", "37", "31" ]
159
5,264
1
false
The dissociation constant for Arp2/3 with the mother strand is ∼50 times higher than with the daughter strand, and thus the weakest part of the structure.
[ "37" ]
The dissociation constant for Arp2/3 with the mother strand is ∼50 times higher than with the daughter strand, and thus the weakest part of the structure.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
This separation would then allow the virion to leave the cell.
[ "31", "49", "37", "31" ]
62
5,265
0
false
This separation would then allow the virion to leave the cell.
[]
This separation would then allow the virion to leave the cell.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
The initiation of migration (Figure 4A) may be the result of myosin motors as a part of the virion, and their intermittent function once the vRNPs are physically separated from the actin network.
[ "31", "49", "37", "31" ]
195
5,266
0
false
The initiation of migration (Figure 4A) may be the result of myosin motors as a part of the virion, and their intermittent function once the vRNPs are physically separated from the actin network.
[]
The initiation of migration (Figure 4A) may be the result of myosin motors as a part of the virion, and their intermittent function once the vRNPs are physically separated from the actin network.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
This is supported by our observation of myosin Va colocalization with vRNPs (see Figure 5A and B).
[ "31", "49", "37", "31" ]
98
5,267
0
false
This is supported by our observation of myosin Va colocalization with vRNPs (see Figure 5A and B).
[]
This is supported by our observation of myosin Va colocalization with vRNPs (see Figure 5A and B).
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
Non-directed motion or anomalous diffusion (Figure 4C) may occur for several reasons; for example, if vRNPs lack the ATP necessary to cause directed motion or if the vRNPs are separated from the actin network via the ADF/cofilin mechanism (31) too early and not by mechanical motion, they may be left trapped within the ...
[ "31", "49", "37", "31" ]
336
5,268
1
false
Non-directed motion or anomalous diffusion (Figure 4C) may occur for several reasons; for example, if vRNPs lack the ATP necessary to cause directed motion or if the vRNPs are separated from the actin network via the ADF/cofilin mechanism too early and not by mechanical motion, they may be left trapped within the plasm...
[ "31" ]
Non-directed motion or anomalous diffusion (Figure 4C) may occur for several reasons; for example, if vRNPs lack the ATP necessary to cause directed motion or if the vRNPs are separated from the actin network via the ADF/cofilin mechanism too early and not by mechanical motion, they may be left trapped within the plasm...
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
It is also possible that these virions were released from an infected cell earlier and reabsorbed, or they are involved in genome replication, and not competent for egress.
[ "31", "49", "37", "31" ]
172
5,269
0
false
It is also possible that these virions were released from an infected cell earlier and reabsorbed, or they are involved in genome replication, and not competent for egress.
[]
It is also possible that these virions were released from an infected cell earlier and reabsorbed, or they are involved in genome replication, and not competent for egress.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
Future studies of the protein/vRNP interactions at the membrane are required to reveal the exact reasons of the non-directed motions, and identify conclusively the proteins involved in this process.
[ "31", "49", "37", "31" ]
198
5,270
0
false
Future studies of the protein/vRNP interactions at the membrane are required to reveal the exact reasons of the non-directed motions, and identify conclusively the proteins involved in this process.
[]
Future studies of the protein/vRNP interactions at the membrane are required to reveal the exact reasons of the non-directed motions, and identify conclusively the proteins involved in this process.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
Figure 7.Hypothesized biophysical model of vRNP motion.
[ "31", "49", "37", "31" ]
55
5,271
0
false
Figure 7.Hypothesized biophysical model of vRNP motion.
[]
Figure 7.Hypothesized biophysical model of vRNP motion.
true
true
true
true
true
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
(A) Active myosin V motor proteins, attached to both the viral genomic RNP and the actin network, cause the rotation of the virion and partial separation from the plasma membrane, via their translation along the actin network.
[ "31", "49", "37", "31" ]
226
5,272
0
false
(A) Active myosin V motor proteins, attached to both the viral genomic RNP and the actin network, cause the rotation of the virion and partial separation from the plasma membrane, via their translation along the actin network.
[]
(A) Active myosin V motor proteins, attached to both the viral genomic RNP and the actin network, cause the rotation of the virion and partial separation from the plasma membrane, via their translation along the actin network.
false
false
true
true
false
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
(B and C) Once the virion is partially free from the cell surface due to vigorous motion (B), motor motion could possibly cause daughter actin filaments within the virion to break from the mother filaments at the Arp2/3 protein complex, allowing for rigorous rotation (Figure 3C and Supplementary Movie 2) or (C) migrati...
[ "31", "49", "37", "31" ]
385
5,273
0
false
(B and C) Once the virion is partially free from the cell surface due to vigorous motion (B), motor motion could possibly cause daughter actin filaments within the virion to break from the mother filaments at the Arp2/3 protein complex, allowing for rigorous rotation (Figure 3C and Supplementary Movie 2) or (C) migrati...
[]
(B and C) Once the virion is partially free from the cell surface due to vigorous motion (B), motor motion could possibly cause daughter actin filaments within the virion to break from the mother filaments at the Arp2/3 protein complex, allowing for rigorous rotation or (C) migration along the actin network.
false
false
true
true
false
875
6
DISCUSSION
1
31
[ "B31", "B49", "B37", "B31" ]
17,485,480
pmid-12600310|pmid-10352018|NA|pmid-12600310
(D) Some particles exhibit β€˜non-directed’ or obstructed diffusion (Figure 3C and Supplementary Movie 4), possibly due to the action of the ADF/cofilin complex leaving it embedded in the membrane without the actin network/motors to propel it from the cell.
[ "31", "49", "37", "31" ]
255
5,274
0
false
(D) Some particles exhibit β€˜non-directed’ or obstructed diffusion (Figure 3C and Supplementary Movie 4), possibly due to the action of the ADF/cofilin complex leaving it embedded in the membrane without the actin network/motors to propel it from the cell.
[]
(D) Some particles exhibit β€˜non-directed’ or obstructed diffusion, possibly due to the action of the ADF/cofilin complex leaving it embedded in the membrane without the actin network/motors to propel it from the cell.
false
false
true
true
false
875
7
DISCUSSION
0
null
null
17,485,480
null
Hypothesized biophysical model of vRNP motion.
null
46
5,275
0
false
null
null
Hypothesized biophysical model of vRNP motion.
true
true
true
true
true
876
7
DISCUSSION
0
null
null
17,485,480
null
(A) Active myosin V motor proteins, attached to both the viral genomic RNP and the actin network, cause the rotation of the virion and partial separation from the plasma membrane, via their translation along the actin network.
null
226
5,276
0
false
null
null
(A) Active myosin V motor proteins, attached to both the viral genomic RNP and the actin network, cause the rotation of the virion and partial separation from the plasma membrane, via their translation along the actin network.
false
false
true
true
false
876
7
DISCUSSION
0
null
null
17,485,480
null
(B and C) Once the virion is partially free from the cell surface due to vigorous motion (B), motor motion could possibly cause daughter actin filaments within the virion to break from the mother filaments at the Arp2/3 protein complex, allowing for rigorous rotation (Figure 3C and Supplementary Movie 2) or (C) migrati...
null
385
5,277
0
false
null
null
(B and C) Once the virion is partially free from the cell surface due to vigorous motion (B), motor motion could possibly cause daughter actin filaments within the virion to break from the mother filaments at the Arp2/3 protein complex, allowing for rigorous rotation (Figure 3C and Supplementary Movie 2) or (C) migrati...
false
false
true
true
false
876
7
DISCUSSION
0
null
null
17,485,480
null
(D) Some particles exhibit β€˜non-directed’ or obstructed diffusion (Figure 3C and Supplementary Movie 4), possibly due to the action of the ADF/cofilin complex leaving it embedded in the membrane without the actin network/motors to propel it from the cell.
null
255
5,278
0
false
null
null
(D) Some particles exhibit β€˜non-directed’ or obstructed diffusion (Figure 3C and Supplementary Movie 4), possibly due to the action of the ADF/cofilin complex leaving it embedded in the membrane without the actin network/motors to propel it from the cell.
false
false
true
true
false
876
0
INTRODUCTION
0
null
null
17,459,890
null
In the context of the Human Genome Project, new technologies have emerged allowing the simultaneous analysis of a large number of genes in a single experiment.
null
159
5,279
0
false
null
null
In the context of the Human Genome Project, new technologies have emerged allowing the simultaneous analysis of a large number of genes in a single experiment.
true
true
true
true
true
877
0
INTRODUCTION
0
null
null
17,459,890
null
The so-called DNA micro-arrays or DNA chips constitute a prominent example.
null
75
5,280
0
false
null
null
The so-called DNA micro-arrays or DNA chips constitute a prominent example.
true
true
true
true
true
877
0
INTRODUCTION
0
null
null
17,459,890
null
The goal of many of these experiments is to identify differentially expressed genes in cultured cells or tissue samples under different physiological or pathological conditions.
null
177
5,281
0
false
null
null
The goal of many of these experiments is to identify differentially expressed genes in cultured cells or tissue samples under different physiological or pathological conditions.
true
true
true
true
true
877
0
INTRODUCTION
0
null
null
17,459,890
null
RNA expression differences are often determined by calculating the ratios of hybridization signals between a test and a reference sample.
null
137
5,282
0
false
null
null
RNA expression differences are often determined by calculating the ratios of hybridization signals between a test and a reference sample.
true
true
true
true
true
877
0
INTRODUCTION
0
null
null
17,459,890
null
One of the characteristics of expression profiling is that, in a typical experiment, thousands of genes are analyzed on a small number of experimental conditions.
null
162
5,283
0
false
null
null
One of the characteristics of expression profiling is that, in a typical experiment, thousands of genes are analyzed on a small number of experimental conditions.
true
true
true
true
true
877
0
INTRODUCTION
0
null
null
17,459,890
null
However, it has proved challenging to identify genuine expression differences while simultaneously avoiding false positives.
null
124
5,284
0
false
null
null
However, it has proved challenging to identify genuine expression differences while simultaneously avoiding false positives.
true
true
true
true
true
877
1
INTRODUCTION
0
null
null
17,459,890
NA
Since the variance is inversely proportional to the log of the signal intensities, and the signal intensities range from 1 (rare) to 105 (strongly expressed genes), although the noise is the same for each gene analyzed on a given array, the noise has more impact on the large majority of weakly expressed genes than on t...
null
366
5,285
0
false
null
null
Since the variance is inversely proportional to the log of the signal intensities, and the signal intensities range from 1 (rare) to 105 (strongly expressed genes), although the noise is the same for each gene analyzed on a given array, the noise has more impact on the large majority of weakly expressed genes than on t...
true
true
true
true
true
878
2
INTRODUCTION
1
1
[ "B1", "B2", "B3 B4 B5", "B6", "B7" ]
17,459,890
NA|NA|pmid-12169537|pmid-12111897|pmid-12169536|pmid-11470887|pmid-10445855
The variation on these experiments has many components, including the variability of the biological samples, labeling conditions, array specificity, reading efficiency for each spot, etc.
[ "1", "2", "3–5", "6", "7" ]
187
5,286
0
false
The variation on these experiments has many components, including the variability of the biological samples, labeling conditions, array specificity, reading efficiency for each spot, etc.
[]
The variation on these experiments has many components, including the variability of the biological samples, labeling conditions, array specificity, reading efficiency for each spot, etc.
true
true
true
true
true
879
2
INTRODUCTION
1
1
[ "B1", "B2", "B3 B4 B5", "B6", "B7" ]
17,459,890
NA|NA|pmid-12169537|pmid-12111897|pmid-12169536|pmid-11470887|pmid-10445855
These variations can be categorized as systematic variation, which can easily be corrected for, and are referred to as calibration and normalization as discussed by Balding et al.
[ "1", "2", "3–5", "6", "7" ]
179
5,287
0
false
These variations can be categorized as systematic variation, which can easily be corrected for, and are referred to as calibration and normalization as discussed by Balding et al.
[]
These variations can be categorized as systematic variation, which can easily be corrected for, and are referred to as calibration and normalization as discussed by Balding et al.
true
true
true
true
true
879
2
INTRODUCTION
1
1
[ "B1", "B2", "B3 B4 B5", "B6", "B7" ]
17,459,890
NA|NA|pmid-12169537|pmid-12111897|pmid-12169536|pmid-11470887|pmid-10445855
Other variations, however, are random, and may be accounted for through error models.
[ "1", "2", "3–5", "6", "7" ]
85
5,288
0
false
Other variations, however, are random, and may be accounted for through error models.
[]
Other variations, however, are random, and may be accounted for through error models.
true
true
true
true
true
879
2
INTRODUCTION
1
2
[ "B1", "B2", "B3 B4 B5", "B6", "B7" ]
17,459,890
NA|NA|pmid-12169537|pmid-12111897|pmid-12169536|pmid-11470887|pmid-10445855
Several models have been used with more or less success, to correct for random variation, including a model based on the generalized logarithm (glog) (2), which has been applied by a number of authors to stabilize the variance (3–5).
[ "1", "2", "3–5", "6", "7" ]
233
5,289
1
false
Several models have been used with more or less success, to correct for random variation, including a model based on the generalized logarithm (glog), which has been applied by a number of authors to stabilize the variance.
[ "2", "3–5" ]
Several models have been used with more or less success, to correct for random variation, including a model based on the generalized logarithm (glog), which has been applied by a number of authors to stabilize the variance.
true
true
true
true
true
879
2
INTRODUCTION
1
6
[ "B1", "B2", "B3 B4 B5", "B6", "B7" ]
17,459,890
NA|NA|pmid-12169537|pmid-12111897|pmid-12169536|pmid-11470887|pmid-10445855
Alternative approaches have been proposed such as noise filtering look up tables (LUT) (6), which uses a scoring system in which a given context will provide predictive values for reproducibility in fold change (FC) results (7).
[ "1", "2", "3–5", "6", "7" ]
228
5,290
1
false
Alternative approaches have been proposed such as noise filtering look up tables (LUT), which uses a scoring system in which a given context will provide predictive values for reproducibility in fold change (FC) results.
[ "6", "7" ]
Alternative approaches have been proposed such as noise filtering look up tables (LUT), which uses a scoring system in which a given context will provide predictive values for reproducibility in fold change (FC) results.
true
true
true
true
true
879
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
Here, we adapt the LMS approach, originally described by Cole and Green (8), to model gene expression profiling data.
[ "8" ]
117
5,291
1
false
Here, we adapt the LMS approach, originally described by Cole and Green, to model gene expression profiling data.
[ "8" ]
Here, we adapt the LMS approach, originally described by Cole and Green, to model gene expression profiling data.
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
The authors initially used this approach for growth charts of children.
[ "8" ]
71
5,292
0
false
The authors initially used this approach for growth charts of children.
[]
The authors initially used this approach for growth charts of children.
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
LMS allows to construct smoothing reference centile curves, which fit cubic spline curves to the Box–Cox transformation.
[ "8" ]
120
5,293
0
false
LMS allows to construct smoothing reference centile curves, which fit cubic spline curves to the Box–Cox transformation.
[]
LMS allows to construct smoothing reference centile curves, which fit cubic spline curves to the Box–Cox transformation.
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
This transformation leads to normalization of the variance and thus defines standard intervals for significant expression differences.
[ "8" ]
134
5,294
0
false
This transformation leads to normalization of the variance and thus defines standard intervals for significant expression differences.
[]
This transformation leads to normalization of the variance and thus defines standard intervals for significant expression differences.
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
Using this transformation, a confidence band adjusted to the actual distribution of the data is defined, which identifies the set of genes devoid of expression differences.
[ "8" ]
172
5,295
0
false
Using this transformation, a confidence band adjusted to the actual distribution of the data is defined, which identifies the set of genes devoid of expression differences.
[]
Using this transformation, a confidence band adjusted to the actual distribution of the data is defined, which identifies the set of genes devoid of expression differences.
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
The confidence band was determined after applying a spline fit, determining the median axis of the plot and the spline curves defining the lower and upper limits of the dispersion space (DS).
[ "8" ]
191
5,296
0
false
The confidence band was determined after applying a spline fit, determining the median axis of the plot and the spline curves defining the lower and upper limits of the dispersion space (DS).
[]
The confidence band was determined after applying a spline fit, determining the median axis of the plot and the spline curves defining the lower and upper limits of the dispersion space (DS).
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
Expression variation (EV) measurements are then based on the size of this DS.
[ "8" ]
77
5,297
0
false
Expression variation (EV) measurements are then based on the size of this DS.
[]
Expression variation (EV) measurements are then based on the size of this DS.
true
true
true
true
true
880
3
INTRODUCTION
1
8
[ "B8" ]
17,459,890
pmid-1518992
Here, we present the application of this method, and comparisons with other strategies, on a dataset generated using the Affymetrix platform.
[ "8" ]
141
5,298
0
false
Here, we present the application of this method, and comparisons with other strategies, on a dataset generated using the Affymetrix platform.
[]
Here, we present the application of this method, and comparisons with other strategies, on a dataset generated using the Affymetrix platform.
true
true
true
true
true
880
0
DISCUSSION
0
null
null
17,459,890
null
In expression profiling analyses, outlining proper thresholds to determine differential expression, avoiding false positives, still remains one of the major issues to be solved.
null
177
5,299
0
false
null
null
In expression profiling analyses, outlining proper thresholds to determine differential expression, avoiding false positives, still remains one of the major issues to be solved.
true
true
true
true
true
881