Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
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 Community
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2
INTRODUCTION
1
11–13
[ "B11 B12 B13", "B14", "B15", "B15 B16 B17 B18", "B19", "B20" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
PcG proteins are epigenetic chromatin modifiers involved in transcription regulation, maintenance of embryonic and adult stem cells and cancer development (14,15).
[ "11–13", "14", "15", "15–18", "19", "20" ]
163
40,616
0
false
PcG proteins are epigenetic chromatin modifiers involved in transcription regulation, maintenance of embryonic and adult stem cells and cancer development.
[ "14,15" ]
PcG proteins are epigenetic chromatin modifiers involved in transcription regulation, maintenance of embryonic and adult stem cells and cancer development.
true
true
true
true
true
7,006
2
INTRODUCTION
1
11–13
[ "B11 B12 B13", "B14", "B15", "B15 B16 B17 B18", "B19", "B20" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
PcG genes were first identified by their requirement for the maintenance of the stable repression of Hox genes during the development of Drosophila melanogaster and are highly conserved throughout evolution.
[ "11–13", "14", "15", "15–18", "19", "20" ]
207
40,617
0
false
PcG genes were first identified by their requirement for the maintenance of the stable repression of Hox genes during the development of Drosophila melanogaster and are highly conserved throughout evolution.
[]
PcG genes were first identified by their requirement for the maintenance of the stable repression of Hox genes during the development of Drosophila melanogaster and are highly conserved throughout evolution.
true
true
true
true
true
7,006
2
INTRODUCTION
1
11–13
[ "B11 B12 B13", "B14", "B15", "B15 B16 B17 B18", "B19", "B20" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
In mammals, PcG genes are also implicated in Homeobox (Hox) gene regulation.
[ "11–13", "14", "15", "15–18", "19", "20" ]
76
40,618
0
false
In mammals, PcG genes are also implicated in Homeobox (Hox) gene regulation.
[]
In mammals, PcG genes are also implicated in Homeobox (Hox) gene regulation.
true
true
true
true
true
7,006
2
INTRODUCTION
1
11–13
[ "B11 B12 B13", "B14", "B15", "B15 B16 B17 B18", "B19", "B20" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Their biological activity lies in stable silencing of specific sets of genes through chromatin modifications.
[ "11–13", "14", "15", "15–18", "19", "20" ]
109
40,619
0
false
Their biological activity lies in stable silencing of specific sets of genes through chromatin modifications.
[]
Their biological activity lies in stable silencing of specific sets of genes through chromatin modifications.
true
true
true
true
true
7,006
2
INTRODUCTION
1
15–18
[ "B11 B12 B13", "B14", "B15", "B15 B16 B17 B18", "B19", "B20" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Recently, emerging evidence implicates the PcG proteins in cellular proliferation and tumorigenesis (15–18).
[ "11–13", "14", "15", "15–18", "19", "20" ]
108
40,620
1
false
Recently, emerging evidence implicates the PcG proteins in cellular proliferation and tumorigenesis.
[ "15–18" ]
Recently, emerging evidence implicates the PcG proteins in cellular proliferation and tumorigenesis.
true
true
true
true
true
7,006
2
INTRODUCTION
1
19
[ "B11 B12 B13", "B14", "B15", "B15 B16 B17 B18", "B19", "B20" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Furthermore, overexpression of a PcG protein, EZH2, in breast epithelial cells reduced Rad51 paralogs both in the mRNA and protein levels which are required for proper HR DNA repair (19), and heterozygosity for mutations in either extra sex combs (Esc) or Enhance of Polycomb [E(PC)] increases the lever of HR and enhanc...
[ "11–13", "14", "15", "15–18", "19", "20" ]
381
40,621
1
false
Furthermore, overexpression of a PcG protein, EZH2, in breast epithelial cells reduced Rad51 paralogs both in the mRNA and protein levels which are required for proper HR DNA repair, and heterozygosity for mutations in either extra sex combs (Esc) or Enhance of Polycomb [E(PC)] increases the lever of HR and enhances ge...
[ "19", "20" ]
Furthermore, overexpression of a PcG protein, EZH2, in breast epithelial cells reduced Rad51 paralogs both in the mRNA and protein levels which are required for proper HR DNA repair, and heterozygosity for mutations in either extra sex combs (Esc) or Enhance of Polycomb [E(PC)] increases the lever of HR and enhances ge...
true
true
true
true
true
7,006
3
INTRODUCTION
1
11
[ "B11", "B12", "B21" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Laser micro-irradiation makes it possible to introduce various types of DNA damage at restricted regions in the nucleus of a single cell and to analyze the response of proteins to the damage with antibody by immuno-staining or with transfected GFP-tagged proteins under microscope in a real-time image.
[ "11", "12", "21" ]
302
40,622
0
false
Laser micro-irradiation makes it possible to introduce various types of DNA damage at restricted regions in the nucleus of a single cell and to analyze the response of proteins to the damage with antibody by immuno-staining or with transfected GFP-tagged proteins under microscope in a real-time image.
[]
Laser micro-irradiation makes it possible to introduce various types of DNA damage at restricted regions in the nucleus of a single cell and to analyze the response of proteins to the damage with antibody by immuno-staining or with transfected GFP-tagged proteins under microscope in a real-time image.
true
true
true
true
true
7,007
3
INTRODUCTION
1
11
[ "B11", "B12", "B21" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
So far as laser light dose and exposed time are extremely limited, there is no effect of heat production.
[ "11", "12", "21" ]
105
40,623
0
false
So far as laser light dose and exposed time are extremely limited, there is no effect of heat production.
[]
So far as laser light dose and exposed time are extremely limited, there is no effect of heat production.
true
true
true
true
true
7,007
3
INTRODUCTION
1
11
[ "B11", "B12", "B21" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
The major product of irradiation with UVA laser light is DNA damage, because UVA laser light is either absorbed directly by DNA or by photosensitizers around DNA creating radicals, which attack DNA.
[ "11", "12", "21" ]
198
40,624
0
false
The major product of irradiation with UVA laser light is DNA damage, because UVA laser light is either absorbed directly by DNA or by photosensitizers around DNA creating radicals, which attack DNA.
[]
The major product of irradiation with UVA laser light is DNA damage, because UVA laser light is either absorbed directly by DNA or by photosensitizers around DNA creating radicals, which attack DNA.
true
true
true
true
true
7,007
3
INTRODUCTION
1
11
[ "B11", "B12", "B21" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Therefore, major types of DNA damage produced by UVA laser irradiation are oxidative ones, and with increasing laser dose and by addition of photosensitizers, DNA single-strand breaks, DSBs and base damage can be produced effectively (11,12,21).
[ "11", "12", "21" ]
245
40,625
0
false
Therefore, major types of DNA damage produced by UVA laser irradiation are oxidative ones, and with increasing laser dose and by addition of photosensitizers, DNA single-strand breaks, DSBs and base damage can be produced effectively.
[ "11,12,21" ]
Therefore, major types of DNA damage produced by UVA laser irradiation are oxidative ones, and with increasing laser dose and by addition of photosensitizers, DNA single-strand breaks, DSBs and base damage can be produced effectively.
true
true
true
true
true
7,007
4
INTRODUCTION
0
null
null
18,385,154
null
For the reason that both PcG proteins play important roles in tumorigenesis, and some of PcG proteins are shown by genetic analysis to be involved in DSBs damage response, we analyzed whether or not PcG proteins are directly involved in the response to DSBs.
null
258
40,626
0
false
null
null
For the reason that both PcG proteins play important roles in tumorigenesis, and some of PcG proteins are shown by genetic analysis to be involved in DSBs damage response, we analyzed whether or not PcG proteins are directly involved in the response to DSBs.
true
true
true
true
true
7,008
4
INTRODUCTION
0
null
null
18,385,154
null
We found that PHD finger Protein 1 (PHF1) is recruited rapidly to DSBs sites, that is dependent on Ku70/Ku80, and demonstrated that PHF1 is associated with Ku70/Ku80, Rad50, DHX9, SMC1 and P53 besides PcG proteins.
null
214
40,627
0
false
null
null
We found that PHD finger Protein 1 (PHF1) is recruited rapidly to DSBs sites, that is dependent on Ku70/Ku80, and demonstrated that PHF1 is associated with Ku70/Ku80, Rad50, DHX9, SMC1 and P53 besides PcG proteins.
true
true
true
true
true
7,008
4
INTRODUCTION
0
null
null
18,385,154
null
Furthermore, knockdown of PHF1 leads to X-ray sensitivity and increases HR frequency.
null
85
40,628
0
false
null
null
Furthermore, knockdown of PHF1 leads to X-ray sensitivity and increases HR frequency.
true
true
true
true
true
7,008
4
INTRODUCTION
0
null
null
18,385,154
null
These data suggest that PHF1 is involved in DSBs damage response and may play an important role in NHEJ and maintaining genome stability.
null
137
40,629
0
false
null
null
These data suggest that PHF1 is involved in DSBs damage response and may play an important role in NHEJ and maintaining genome stability.
true
true
true
true
true
7,008
0
DISCUSSION
1
34
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
PcG proteins are epigenetic chromatin modifiers involved in gene silencing, cancer development and the maintenance of embryonic and adult stem cells.
[ "34", "35", "36" ]
149
40,630
0
false
PcG proteins are epigenetic chromatin modifiers involved in gene silencing, cancer development and the maintenance of embryonic and adult stem cells.
[]
PcG proteins are epigenetic chromatin modifiers involved in gene silencing, cancer development and the maintenance of embryonic and adult stem cells.
true
true
true
true
true
7,009
0
DISCUSSION
1
34
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
During screening of proteins responding to DNA damage by using laser micro-irradiation we found that mPcl1, a mouse homolog of polycomb-like protein (Pcl) of the D. melanogaster, accumulates at laser-irradiated site.
[ "34", "35", "36" ]
216
40,631
0
false
During screening of proteins responding to DNA damage by using laser micro-irradiation we found that mPcl1, a mouse homolog of polycomb-like protein (Pcl) of the D. melanogaster, accumulates at laser-irradiated site.
[]
During screening of proteins responding to DNA damage by using laser micro-irradiation we found that mPcl1, a mouse homolog of polycomb-like protein (Pcl) of the D. melanogaster, accumulates at laser-irradiated site.
true
true
true
true
true
7,009
0
DISCUSSION
1
34
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
It was previously shown that the Drosophila Pcl protein is associated with a 1 MDa complex containing the members of PcG, ESC, E(Z), SU(Z)12, p55 and RPD3, and, furthermore.
[ "34", "35", "36" ]
173
40,632
0
false
It was previously shown that the Drosophila Pcl protein is associated with a 1 MDa complex containing the members of PcG, ESC, E(Z), SU(Z)12, p55 and RPD3, and, furthermore.
[]
It was previously shown that the Drosophila Pcl protein is associated with a 1 MDa complex containing the members of PcG, ESC, E(Z), SU(Z)12, p55 and RPD3, and, furthermore.
true
true
true
true
true
7,009
0
DISCUSSION
1
34
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
Pcl is present in another unknown complex, which does not contain any component of the 600 kDa ESC/E(Z) complex (34).
[ "34", "35", "36" ]
117
40,633
1
false
Pcl is present in another unknown complex, which does not contain any component of the 600 kDa ESC/E(Z) complex.
[ "34" ]
Pcl is present in another unknown complex, which does not contain any component of the 600 kDa ESC/E(Z) complex.
true
true
true
true
true
7,009
0
DISCUSSION
1
34
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
Therefore, we were interested in the mechanisms and function of the damage response and isolated a human homolog of Pcl1, PHF1.
[ "34", "35", "36" ]
127
40,634
0
false
Therefore, we were interested in the mechanisms and function of the damage response and isolated a human homolog of Pcl1, PHF1.
[]
Therefore, we were interested in the mechanisms and function of the damage response and isolated a human homolog of Pcl1, PHF1.
true
true
true
true
true
7,009
0
DISCUSSION
1
35
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
PHF1 was identified several years ago as a human homolog of Drosophila Pcl that locates on 6p21.3, belonging to MHC classII region (35).
[ "34", "35", "36" ]
136
40,635
1
false
PHF1 was identified several years ago as a human homolog of Drosophila Pcl that locates on 6p21.3, belonging to MHC classII region.
[ "35" ]
PHF1 was identified several years ago as a human homolog of Drosophila Pcl that locates on 6p21.3, belonging to MHC classII region.
true
true
true
true
true
7,009
0
DISCUSSION
1
36
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
Although PHF1 shares significant sequence similarity to the Drosophila Pcl, and was shown recently to be involved in tumorigenesis and/or tumor progression of endometrial stromal sarcoma by genetic analysis (36), its function was still unknown.
[ "34", "35", "36" ]
244
40,636
1
false
Although PHF1 shares significant sequence similarity to the Drosophila Pcl, and was shown recently to be involved in tumorigenesis and/or tumor progression of endometrial stromal sarcoma by genetic analysis, its function was still unknown.
[ "36" ]
Although PHF1 shares significant sequence similarity to the Drosophila Pcl, and was shown recently to be involved in tumorigenesis and/or tumor progression of endometrial stromal sarcoma by genetic analysis, its function was still unknown.
true
true
true
true
true
7,009
0
DISCUSSION
1
34
[ "B34", "B35", "B36" ]
18,385,154
pmid-16807135|pmid-11242102|pmid-12186839|pmid-12846809|pmid-12947387|pmid-15189136|pmid-8700231|pmid-12697833|pmid-9545646|pmid-16397222
In this study, we identified PHF1 as a new component in DSBs damage response, possibly involved in the repair pathway of DSBs.
[ "34", "35", "36" ]
126
40,637
0
false
In this study, we identified PHF1 as a new component in DSBs damage response, possibly involved in the repair pathway of DSBs.
[]
In this study, we identified PHF1 as a new component in DSBs damage response, possibly involved in the repair pathway of DSBs.
true
true
true
true
true
7,009
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
Endogenous and GFP-tagged PHF1 were recruited immediately to the sites irradiated with laser micro-irradiation in HeLa cells (Figure 1).
null
136
40,638
0
false
null
null
Endogenous and GFP-tagged PHF1 were recruited immediately to the sites irradiated with laser micro-irradiation in HeLa cells (Figure 1).
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
Since PHF1 accumulates at the site irradiated with higher laser dose in the presence of BrdU and PHF1 neither accumulates at the site irradiated with lower laser dose of the laser light, nor responds to UV-irradiation (data not shown), PHF1 is possibly involved in DSBs damage response.
null
286
40,639
0
false
null
null
Since PHF1 accumulates at the site irradiated with higher laser dose in the presence of BrdU and PHF1 neither accumulates at the site irradiated with lower laser dose of the laser light, nor responds to UV-irradiation (data not shown), PHF1 is possibly involved in DSBs damage response.
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
Although the response of endogenous PHF1 was only weakly shown by antibody (Figure 1), it is probably due to low sensitivity of the antibody and/or rapid dissociation of PHF1 from the accumulated sites as shown by the results obtained with GFP-tagged protein (Figure 1C).
null
271
40,640
0
false
null
null
Although the response of endogenous PHF1 was only weakly shown by antibody (Figure 1), it is probably due to low sensitivity of the antibody and/or rapid dissociation of PHF1 from the accumulated sites as shown by the results obtained with GFP-tagged protein (Figure 1C).
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
PHF1 did not accumulate at irradiated sites in Ku-deficient XR-V15B cells, but it accumulated in V15B cells expressing Ku80 (Figure 3B).
null
136
40,641
0
false
null
null
PHF1 did not accumulate at irradiated sites in Ku-deficient XR-V15B cells, but it accumulated in V15B cells expressing Ku80 (Figure 3B).
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
These data suggest that PHF1 is recruited under the influence of Ku proteins to DSBs.
null
85
40,642
0
false
null
null
These data suggest that PHF1 is recruited under the influence of Ku proteins to DSBs.
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
In contrast to Ku-deficient cells, PHF1 accumulation was not affected in other mutant cells, which are defective in DNA-PKcs, XRCC4 and NBS1 (Figure 3C–E).
null
155
40,643
0
false
null
null
In contrast to Ku-deficient cells, PHF1 accumulation was not affected in other mutant cells, which are defective in DNA-PKcs, XRCC4 and NBS1 (Figure 3C–E).
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
We thought, therefore, that there might be some physical and/or functional interactions between PHF1 and Ku70/Ku80.
null
115
40,644
0
false
null
null
We thought, therefore, that there might be some physical and/or functional interactions between PHF1 and Ku70/Ku80.
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
Co-IP experiments showed that PHF1 is indeed physically associated with Ku70/Ku80 (Figure 4A and B).
null
100
40,645
0
false
null
null
Co-IP experiments showed that PHF1 is indeed physically associated with Ku70/Ku80 (Figure 4A and B).
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
In accordance with these data, siRNA-mediated downregulation of PHF1 provided the host HeLa cells with a mild but consistent cell sensitivity to X-ray (Figure 4E).
null
163
40,646
0
false
null
null
In accordance with these data, siRNA-mediated downregulation of PHF1 provided the host HeLa cells with a mild but consistent cell sensitivity to X-ray (Figure 4E).
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
Furthermore, the downregulation of PHF1 leads the cells to an enhanced HR frequency (Figure 4F), which provides further evidence that PHF1 is involved in DSBs repair and supports NHEJ.
null
184
40,647
0
false
null
null
Furthermore, the downregulation of PHF1 leads the cells to an enhanced HR frequency (Figure 4F), which provides further evidence that PHF1 is involved in DSBs repair and supports NHEJ.
true
true
true
true
true
7,010
1
DISCUSSION
0
null
null
18,385,154
pmid-16807135|pmid-12947387|pmid-15175261|pmid-16807135|pmid-12947387|pmid-15175261|pmid-12947387|pmid-12612651|pmid-15989948
Like other NHEJ factors PHF1 is recruited to DSBs sites both G1 and S/G2 phases (Figure 3E).
null
92
40,648
0
false
null
null
Like other NHEJ factors PHF1 is recruited to DSBs sites both G1 and S/G2 phases (Figure 3E).
true
true
true
true
true
7,010
2
DISCUSSION
1
24
[ "B24", "B37 B38 B39 B40" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
PHF1 contains two zinc finger-like PHD domains and a Tudor domain (Figure 2A).
[ "24", "37–40" ]
78
40,649
0
false
PHF1 contains two zinc finger-like PHD domains and a Tudor domain (Figure 2A).
[]
PHF1 contains two zinc finger-like PHD domains and a Tudor domain.
true
true
true
true
true
7,011
2
DISCUSSION
1
24
[ "B24", "B37 B38 B39 B40" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Tudor domain of 53BP1 interacts with methylated histones and mediates its recruitment to DSBs sites (24).
[ "24", "37–40" ]
105
40,650
1
false
Tudor domain of 53BP1 interacts with methylated histones and mediates its recruitment to DSBs sites.
[ "24" ]
Tudor domain of 53BP1 interacts with methylated histones and mediates its recruitment to DSBs sites.
true
true
true
true
true
7,011
2
DISCUSSION
1
37–40
[ "B24", "B37 B38 B39 B40" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Recently, PHD domain was shown to promote both gene expression and repression through an interaction with H3K4me3 tails (37–40).
[ "24", "37–40" ]
128
40,651
1
false
Recently, PHD domain was shown to promote both gene expression and repression through an interaction with H3K4me3 tails.
[ "37–40" ]
Recently, PHD domain was shown to promote both gene expression and repression through an interaction with H3K4me3 tails.
true
true
true
true
true
7,011
2
DISCUSSION
1
24
[ "B24", "B37 B38 B39 B40" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
The data reported here showed that PHD domain is not responsible for the recruitment of PHF1 to DSBs sites, while Tudor domain and central region of PHF1 (310–431) are responsible for the recruitment (Figure 2A).
[ "24", "37–40" ]
212
40,652
0
false
The data reported here showed that PHD domain is not responsible for the recruitment of PHF1 to DSBs sites, while Tudor domain and central region of PHF1 (310–431) are responsible for the recruitment (Figure 2A).
[]
The data reported here showed that PHD domain is not responsible for the recruitment of PHF1 to DSBs sites, while Tudor domain and central region of PHF1 are responsible for the recruitment.
true
true
true
true
true
7,011
2
DISCUSSION
1
24
[ "B24", "B37 B38 B39 B40" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Preliminary data suggest that neither of the domains interacts with Ku80 (data not shown) but the whole PHF1 interacts with Ku proteins, suggesting that accumulation of PHF1 at DSBs requires Ku protein and the two domains have individual binding activities to DSBs.
[ "24", "37–40" ]
265
40,653
0
false
Preliminary data suggest that neither of the domains interacts with Ku80 (data not shown) but the whole PHF1 interacts with Ku proteins, suggesting that accumulation of PHF1 at DSBs requires Ku protein and the two domains have individual binding activities to DSBs.
[]
Preliminary data suggest that neither of the domains interacts with Ku80 (data not shown) but the whole PHF1 interacts with Ku proteins, suggesting that accumulation of PHF1 at DSBs requires Ku protein and the two domains have individual binding activities to DSBs.
true
true
true
true
true
7,011
2
DISCUSSION
1
24
[ "B24", "B37 B38 B39 B40" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17439963|pmid-12628181|pmid-15315754|pmid-15315754|pmid-17060944|pmid-17374722|pmid-17560333|pmid-16855786|pmid-16452150|pmid-15525939|pmid-16728978|pmid-16728976|pmid-16728974|pmid-16728977
Further analysis is necessary to understand the mechanisms behind the recruitment of PHF1 to DSBs.
[ "24", "37–40" ]
98
40,654
0
false
Further analysis is necessary to understand the mechanisms behind the recruitment of PHF1 to DSBs.
[]
Further analysis is necessary to understand the mechanisms behind the recruitment of PHF1 to DSBs.
true
true
true
true
true
7,011
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Besides Ku70/Ku80, PHF1 is also associated with various proteins involved in the response to DSBs and other genomic maintenance mechanisms, Rad50, DHX9, SMC1 and p53 (Figure 5).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
177
40,655
0
false
Besides Ku70/Ku80, PHF1 is also associated with various proteins involved in the response to DSBs and other genomic maintenance mechanisms, Rad50, DHX9, SMC1 and p53 (Figure 5).
[]
Besides Ku70/Ku80, PHF1 is also associated with various proteins involved in the response to DSBs and other genomic maintenance mechanisms, Rad50, DHX9, SMC1 and p53 (Figure 5).
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Rad50 forms a complex with Mre11 and NBS1 that is essential in maintaining DNA integrity by functioning in DSBs repair, meiotic recombination and telomere maintenance (31–33).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
175
40,656
1
false
Rad50 forms a complex with Mre11 and NBS1 that is essential in maintaining DNA integrity by functioning in DSBs repair, meiotic recombination and telomere maintenance.
[ "31–33" ]
Rad50 forms a complex with Mre11 and NBS1 that is essential in maintaining DNA integrity by functioning in DSBs repair, meiotic recombination and telomere maintenance.
true
true
true
true
true
7,012
3
DISCUSSION
1
41–44
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
SMC1 and SMC3 constitute the core of the cohesion complexes which play an important role in the repair of DNA DSBs from yeast to human, and SMC1 and SMC3 also interact with Mre11-Rad50 (41–44).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
193
40,657
1
false
SMC1 and SMC3 constitute the core of the cohesion complexes which play an important role in the repair of DNA DSBs from yeast to human, and SMC1 and SMC3 also interact with Mre11-Rad50.
[ "41–44" ]
SMC1 and SMC3 constitute the core of the cohesion complexes which play an important role in the repair of DNA DSBs from yeast to human, and SMC1 and SMC3 also interact with Mre11-Rad50.
true
true
true
true
true
7,012
3
DISCUSSION
1
45
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
DHX9 is a DNA- and RNA-dependent helicase associated directly with γ-H2AX (45).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
79
40,658
1
false
DHX9 is a DNA- and RNA-dependent helicase associated directly with γ-H2AX.
[ "45" ]
DHX9 is a DNA- and RNA-dependent helicase associated directly with γ-H2AX.
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
As a tumor suppressor, p53 plays a central role in the DNA damage response involved in multiple signaling pathways (46,47).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
123
40,659
0
false
As a tumor suppressor, p53 plays a central role in the DNA damage response involved in multiple signaling pathways.
[ "46,47" ]
As a tumor suppressor, p53 plays a central role in the DNA damage response involved in multiple signaling pathways.
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Rad50, SMC1 and p53 were also demonstrated to be recruited to DSBs sites induced by laser micro-irradiation (44,48).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
116
40,660
0
false
Rad50, SMC1 and p53 were also demonstrated to be recruited to DSBs sites induced by laser micro-irradiation.
[ "44,48" ]
Rad50, SMC1 and p53 were also demonstrated to be recruited to DSBs sites induced by laser micro-irradiation.
true
true
true
true
true
7,012
3
DISCUSSION
1
41–43
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
The SMC1/SMC3 cohesion complex facilitates DSBs repair by HR and holds sister chromatids together locally at DSBs to allow strand invasion and exchange with the sister chromatid template (41–43).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
195
40,661
1
false
The SMC1/SMC3 cohesion complex facilitates DSBs repair by HR and holds sister chromatids together locally at DSBs to allow strand invasion and exchange with the sister chromatid template.
[ "41–43" ]
The SMC1/SMC3 cohesion complex facilitates DSBs repair by HR and holds sister chromatids together locally at DSBs to allow strand invasion and exchange with the sister chromatid template.
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Defect in Rad50 influences phosphorylation of SMC1 and reduces HR (31,49).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
74
40,662
0
false
Defect in Rad50 influences phosphorylation of SMC1 and reduces HR.
[ "31,49" ]
Defect in Rad50 influences phosphorylation of SMC1 and reduces HR.
true
true
true
true
true
7,012
3
DISCUSSION
1
50
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Moreover, another binding protein of PHF1, RbAp46 is a component of histone deacetylase complexes and is involved in chromatin remodeling, interacts with BRCA1 (50).
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
165
40,663
1
false
Moreover, another binding protein of PHF1, RbAp46 is a component of histone deacetylase complexes and is involved in chromatin remodeling, interacts with BRCA1.
[ "50" ]
Moreover, another binding protein of PHF1, RbAp46 is a component of histone deacetylase complexes and is involved in chromatin remodeling, interacts with BRCA1.
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
These data suggest that PHF1 may play a role in HR as well.
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
59
40,664
0
false
These data suggest that PHF1 may play a role in HR as well.
[]
These data suggest that PHF1 may play a role in HR as well.
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
Further analysis is required to understand possible functions of PHF1 in the damage response.
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
93
40,665
0
false
Further analysis is required to understand possible functions of PHF1 in the damage response.
[]
Further analysis is required to understand possible functions of PHF1 in the damage response.
true
true
true
true
true
7,012
3
DISCUSSION
1
31–33
[ "B31 B32 B33", "B41 B42 B43 B44", "B45", "B46", "B47", "B44", "B48", "B41 B42 B43", "B31", "B49", "B50" ]
18,385,154
pmid-15365186|pmid-16141234|pmid-17803946|pmid-16087684|pmid-15908798|pmid-15790808|pmid-16876157|pmid-16810316|pmid-15937217|pmid-12228239|pmid-15613478|pmid-17031865|pmid-16805666|pmid-12228239|pmid-16618811|pmid-16876157|pmid-16810316|pmid-15937217|pmid-16087684|pmid-15135728|pmid-10220405
While we do not know the exact function of PHF1 in the DSBs repair yet, we demonstrated for the first time in mammalian cells that PHF1, as known as a PcG protein, is involved in DSBs response and possibly in its repair.
[ "31–33", "41–44", "45", "46", "47", "44", "48", "41–43", "31", "49", "50" ]
220
40,666
0
false
While we do not know the exact function of PHF1 in the DSBs repair yet, we demonstrated for the first time in mammalian cells that PHF1, as known as a PcG protein, is involved in DSBs response and possibly in its repair.
[]
While we do not know the exact function of PHF1 in the DSBs repair yet, we demonstrated for the first time in mammalian cells that PHF1, as known as a PcG protein, is involved in DSBs response and possibly in its repair.
true
true
true
true
true
7,012
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
At present, there are several lines of evidence that permeant ions modulate T-type Ca2+ channel gating.
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
103
40,667
0
false
At present, there are several lines of evidence that permeant ions modulate T-type Ca2+ channel gating.
[]
At present, there are several lines of evidence that permeant ions modulate T-type Ca2+ channel gating.
true
true
true
true
true
7,013
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Shuba et al.
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
12
40,668
0
false
Shuba et al.
[]
Shuba et al.
true
true
true
true
true
7,013
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
(1991) showed that the mean open time of the T-type channel of mouse neuroblastoma cells depends on the type and concentration of the permeant ion and proposed that the channel region controlling channel closure senses a smaller fraction of the electric field due to a dynamic interaction between the ionic flux and the ...
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
339
40,669
0
false
(1991) showed that the mean open time of the T-type channel of mouse neuroblastoma cells depends on the type and concentration of the permeant ion and proposed that the channel region controlling channel closure senses a smaller fraction of the electric field due to a dynamic interaction between the ionic flux and the ...
[]
showed that the mean open time of the T-type channel of mouse neuroblastoma cells depends on the type and concentration of the permeant ion and proposed that the channel region controlling channel closure senses a smaller fraction of the electric field due to a dynamic interaction between the ionic flux and the selecti...
false
true
true
true
false
7,013
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In frog-atrial cardiomyocytes, Alvarez et al.
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
45
40,670
0
false
In frog-atrial cardiomyocytes, Alvarez et al.
[]
In frog-atrial cardiomyocytes, Alvarez et al.
true
true
true
true
true
7,013
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
(2000) showed that channel reopening after strong depolarizing prepulses was enhanced in low-Na+ extracellular solutions and proposed that a competition between Na+ and Ca2+ for binding sites within the channel modulates the amplitude of the voltage-facilitated T-type current.
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
277
40,671
0
false
(2000) showed that channel reopening after strong depolarizing prepulses was enhanced in low-Na+ extracellular solutions and proposed that a competition between Na+ and Ca2+ for binding sites within the channel modulates the amplitude of the voltage-facilitated T-type current.
[]
(2000) showed that channel reopening after strong depolarizing prepulses was enhanced in low-Na+ extracellular solutions and proposed that a competition between Na+ and Ca2+ for binding sites within the channel modulates the amplitude of the voltage-facilitated T-type current.
false
false
true
true
false
7,013
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
It is also known that the α1G clone (CaV3.1) shows faster macroscopic inactivation in the presence of Ba2+ than in Ca2+
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
119
40,672
0
false
It is also known that the α1G clone (CaV3.1) shows faster macroscopic inactivation in the presence of Ba2+ than in Ca2+
[]
It is also known that the α1G clone shows faster macroscopic inactivation in the presence of Ba2+ than in Ca2+
true
true
false
true
false
7,013
0
INTRODUCTION
1
Shuba et al. (1991)
[ "bib26", "bib1", "bib14", "bib28" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
(Klugbauer et al., 1999; Staes et al., 2001).
[ "Shuba et al. (1991)", "Alvarez et al. (2000)", "Klugbauer et al., 1999", "Staes et al., 2001" ]
45
40,673
0
false
.
[ "Klugbauer et al., 1999; Staes et al., 2001" ]
.
false
false
true
true
false
7,013
1
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
T-type Ca2+ channels are also modulated by extracellular protons.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
65
40,674
0
false
T-type Ca2+ channels are also modulated by extracellular protons.
[]
T-type Ca2+ channels are also modulated by extracellular protons.
true
true
true
true
true
7,014
1
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
Tytgat et al.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
13
40,675
0
false
Tytgat et al.
[]
Tytgat et al.
true
true
true
true
true
7,014
1
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
(1990) reported that, in guinea pig cardiomyocytes, low extracellular pH (pHe) induced a positive shift in the voltages for half-maximal activation (V act) and inactivation (V inac) together with an increase in the slope factor of channel activation (s act).
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
258
40,676
0
false
(1990) reported that, in guinea pig cardiomyocytes, low extracellular pH (pHe) induced a positive shift in the voltages for half-maximal activation (V act) and inactivation (V inac) together with an increase in the slope factor of channel activation (s act).
[]
reported that, in guinea pig cardiomyocytes, low extracellular pH induced a positive shift in the voltages for half-maximal activation and inactivation together with an increase in the slope factor of channel activation.
false
true
true
true
false
7,014
1
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
In a recent paper, Delisle and Satin (2000) concluded that proton block of T-type currents (α1H, CaV3.2) under physiological conditions is due to gating modifications.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
167
40,677
0
false
In a recent paper, Delisle and Satin (2000) concluded that proton block of T-type currents (α1H, CaV3.2) under physiological conditions is due to gating modifications.
[]
In a recent paper, Delisle and Satin concluded that proton block of T-type currents under physiological conditions is due to gating modifications.
true
true
true
true
true
7,014
1
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
These authors related the shift in the voltage for half-maximal activation by high extracellular proton concentrations to the titration of negative surface charges.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
164
40,678
0
false
These authors related the shift in the voltage for half-maximal activation by high extracellular proton concentrations to the titration of negative surface charges.
[]
These authors related the shift in the voltage for half-maximal activation by high extracellular proton concentrations to the titration of negative surface charges.
true
true
true
true
true
7,014
1
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
To explain the increased slope factor of activation, they proposed that external acidification titrates some of the charges involved in the voltage sensing, but suggested already to test the influence of intrapore ions on the gating mechanisms.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
244
40,679
0
false
To explain the increased slope factor of activation, they proposed that external acidification titrates some of the charges involved in the voltage sensing, but suggested already to test the influence of intrapore ions on the gating mechanisms.
[]
To explain the increased slope factor of activation, they proposed that external acidification titrates some of the charges involved in the voltage sensing, but suggested already to test the influence of intrapore ions on the gating mechanisms.
true
true
true
true
true
7,014
2
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
Protons have also been shown to affect the open pore conduction of T-type channels.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
83
40,680
0
false
Protons have also been shown to affect the open pore conduction of T-type channels.
[]
Protons have also been shown to affect the open pore conduction of T-type channels.
true
true
true
true
true
7,015
2
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
Tytgat et al.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
13
40,681
0
false
Tytgat et al.
[]
Tytgat et al.
true
true
true
true
true
7,015
2
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
(1990) showed that extracellular acidification from pH 9.0 to 6 decreases the single channel conductance of the cardiac T-type channel of the guinea pig using 110 mM Ca2+ as charge carrier.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
189
40,682
0
false
(1990) showed that extracellular acidification from pH 9.0 to 6 decreases the single channel conductance of the cardiac T-type channel of the guinea pig using 110 mM Ca2+ as charge carrier.
[]
showed that extracellular acidification from pH 9.0 to 6 decreases the single channel conductance of the cardiac T-type channel of the guinea pig using 110 mM Ca2+ as charge carrier.
false
true
true
true
false
7,015
2
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
For the α1H T-type clone, Delisle and Satin (2000) found that open pore block was not the main determinant for the inhibition of whole-cell currents by extracellular protons in the presence of 2.5 mM
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
199
40,683
0
false
For the α1H T-type clone, Delisle and Satin (2000) found that open pore block was not the main determinant for the inhibition of whole-cell currents by extracellular protons in the presence of 2.5 mM
[]
For the α1H T-type clone, Delisle and Satin found that open pore block was not the main determinant for the inhibition of whole-cell currents by extracellular protons in the presence of 2.5 mM
true
true
false
true
false
7,015
2
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
extracellular Ca2+ and 140 mM Na+.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
34
40,684
0
false
extracellular Ca2+ and 140 mM Na+.
[]
extracellular Ca2+ and 140 mM Na+.
false
true
true
true
false
7,015
2
INTRODUCTION
1
Tytgat et al. (1990)
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
They reported a negative shift of the reversal potential after extracellular acidification and suggested that channel protonation shifts channel selectivity toward monovalent ions.
[ "Tytgat et al. (1990)", "Delisle and Satin (2000)" ]
180
40,685
0
false
They reported a negative shift of the reversal potential after extracellular acidification and suggested that channel protonation shifts channel selectivity toward monovalent ions.
[]
They reported a negative shift of the reversal potential after extracellular acidification and suggested that channel protonation shifts channel selectivity toward monovalent ions.
true
true
true
true
true
7,015
3
INTRODUCTION
1
Pietrobon et al., 1989
[ "bib20", "bib22", "bib16", "bib32", "bib36", "bib27", "bib25", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Protons have been shown to modulate the gating processes of high voltage–activated (HVA)* Ca2+ channels (Pietrobon et al., 1989; Prod'hom et al., 1989; Kwan and Kass, 1993; Tombaugh and Somjen, 1996; Zhou and Jones, 1996; Smirnov et al., 2000; Shah et al., 2001).
[ "Pietrobon et al., 1989", "Prod'hom et al., 1989", "Kwan and Kass, 1993", "Tombaugh and Somjen, 1996", "Zhou and Jones, 1996", "Smirnov et al., 2000", "Shah et al., 2001", "Kwan and Kass, 1993", "Chen et al., 1996", "Chen and Tsien, 1997" ]
263
40,686
0
false
Protons have been shown to modulate the gating processes of high voltage–activated (HVA)* Ca2+ channels.
[ "Pietrobon et al., 1989; Prod'hom et al., 1989; Kwan and Kass, 1993; Tombaugh and Somjen, 1996; Zhou and Jones, 1996; Smirnov et al., 2000; Shah et al., 2001" ]
Protons have been shown to modulate the gating processes of high voltage–activated (HVA)* Ca2+ channels.
true
true
true
true
true
7,016
3
INTRODUCTION
1
Kwan and Kass, 1993
[ "bib20", "bib22", "bib16", "bib32", "bib36", "bib27", "bib25", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In these channels, Ca2+ has been shown to be a major competitor of protons for the neutralization of surface charges (Kwan and Kass, 1993) and for binding to pore residues that control ion permeation and selectivity (Chen et al., 1996; Chen and Tsien, 1997).
[ "Pietrobon et al., 1989", "Prod'hom et al., 1989", "Kwan and Kass, 1993", "Tombaugh and Somjen, 1996", "Zhou and Jones, 1996", "Smirnov et al., 2000", "Shah et al., 2001", "Kwan and Kass, 1993", "Chen et al., 1996", "Chen and Tsien, 1997" ]
258
40,687
1
false
In these channels, Ca2+ has been shown to be a major competitor of protons for the neutralization of surface charges and for binding to pore residues that control ion permeation and selectivity.
[ "Kwan and Kass, 1993", "Chen et al., 1996; Chen and Tsien, 1997" ]
In these channels, Ca2+ has been shown to be a major competitor of protons for the neutralization of surface charges and for binding to pore residues that control ion permeation and selectivity.
true
true
true
true
true
7,016
3
INTRODUCTION
1
Pietrobon et al., 1989
[ "bib20", "bib22", "bib16", "bib32", "bib36", "bib27", "bib25", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
To achieve further insight in the mechanisms of ionic modulation of T-type channel function, we investigated the influence of the extracellular Ca2+ concentration ([Ca2+]e) on the proton-induced modifications of gating and permeation mechanisms of the α1G T-type subunit and the role of the selectivity filter (the EEDD ...
[ "Pietrobon et al., 1989", "Prod'hom et al., 1989", "Kwan and Kass, 1993", "Tombaugh and Somjen, 1996", "Zhou and Jones, 1996", "Smirnov et al., 2000", "Shah et al., 2001", "Kwan and Kass, 1993", "Chen et al., 1996", "Chen and Tsien, 1997" ]
399
40,688
0
false
To achieve further insight in the mechanisms of ionic modulation of T-type channel function, we investigated the influence of the extracellular Ca2+ concentration ([Ca2+]e) on the proton-induced modifications of gating and permeation mechanisms of the α1G T-type subunit and the role of the selectivity filter (the EEDD ...
[]
To achieve further insight in the mechanisms of ionic modulation of T-type channel function, we investigated the influence of the extracellular Ca2+ concentration on the proton-induced modifications of gating and permeation mechanisms of the α1G T-type subunit and the role of the selectivity filter in the modulation of...
true
true
true
true
true
7,016
3
INTRODUCTION
1
Pietrobon et al., 1989
[ "bib20", "bib22", "bib16", "bib32", "bib36", "bib27", "bib25", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Our results suggest for three substrates of competition between Ca2+ and protons, resulting in modifications of channel gating and ionic conduction.
[ "Pietrobon et al., 1989", "Prod'hom et al., 1989", "Kwan and Kass, 1993", "Tombaugh and Somjen, 1996", "Zhou and Jones, 1996", "Smirnov et al., 2000", "Shah et al., 2001", "Kwan and Kass, 1993", "Chen et al., 1996", "Chen and Tsien, 1997" ]
148
40,689
0
false
Our results suggest for three substrates of competition between Ca2+ and protons, resulting in modifications of channel gating and ionic conduction.
[]
Our results suggest for three substrates of competition between Ca2+ and protons, resulting in modifications of channel gating and ionic conduction.
true
true
true
true
true
7,016
0
DISCUSSION
1
Prod'hom et al., 1989
[ "bib22", "bib33", "bib26", "bib16", "bib6", "bib21", "bib1", "bib8", "bib25", "bib33", "bib8", "bib25", "bib33", "bib8", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Both gating and permeation mechanisms of voltage-dependent Ca2+ channels are modulated by ionic conditions and particularly by extracellular protons and Ca2+ (Prod'hom et al., 1989; Tytgat et al., 1990; Shuba et al., 1991; Kwan and Kass, 1993; Chen et al., 1996; Polo-Parada and Korn, 1997; Alvarez et al., 2000; Delisle...
[ "Prod'hom et al., 1989", "Tytgat et al., 1990", "Shuba et al., 1991", "Kwan and Kass, 1993", "Chen et al., 1996", "Polo-Parada and Korn, 1997", "Alvarez et al., 2000", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytgat et al., 1990", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytg...
357
40,690
0
false
Both gating and permeation mechanisms of voltage-dependent Ca2+ channels are modulated by ionic conditions and particularly by extracellular protons and Ca2+.
[ "Prod'hom et al., 1989; Tytgat et al., 1990; Shuba et al., 1991; Kwan and Kass, 1993; Chen et al., 1996; Polo-Parada and Korn, 1997; Alvarez et al., 2000; Delisle and Satin, 2000; Shah et al., 2001" ]
Both gating and permeation mechanisms of voltage-dependent Ca2+ channels are modulated by ionic conditions and particularly by extracellular protons and Ca2+.
true
true
true
true
true
7,017
0
DISCUSSION
1
Prod'hom et al., 1989
[ "bib22", "bib33", "bib26", "bib16", "bib6", "bib21", "bib1", "bib8", "bib25", "bib33", "bib8", "bib25", "bib33", "bib8", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
For T-type Ca2+ channels in particular it has been shown previously that extracellular protons shift the voltage dependence of channel activation due to neutralization of surface charges and decrease the voltage sensitivity of channel activation, which is not consistent with the surface charge hypothesis (Tytgat et al....
[ "Prod'hom et al., 1989", "Tytgat et al., 1990", "Shuba et al., 1991", "Kwan and Kass, 1993", "Chen et al., 1996", "Polo-Parada and Korn, 1997", "Alvarez et al., 2000", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytgat et al., 1990", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytg...
372
40,691
0
false
For T-type Ca2+ channels in particular it has been shown previously that extracellular protons shift the voltage dependence of channel activation due to neutralization of surface charges and decrease the voltage sensitivity of channel activation, which is not consistent with the surface charge hypothesis.
[ "Tytgat et al., 1990; Delisle and Satin, 2000; Shah et al., 2001" ]
For T-type Ca2+ channels in particular it has been shown previously that extracellular protons shift the voltage dependence of channel activation due to neutralization of surface charges and decrease the voltage sensitivity of channel activation, which is not consistent with the surface charge hypothesis.
true
true
true
true
true
7,017
0
DISCUSSION
1
Prod'hom et al., 1989
[ "bib22", "bib33", "bib26", "bib16", "bib6", "bib21", "bib1", "bib8", "bib25", "bib33", "bib8", "bib25", "bib33", "bib8", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In addition, protons either reduce single channel conductance in 110 mM Ca2+
[ "Prod'hom et al., 1989", "Tytgat et al., 1990", "Shuba et al., 1991", "Kwan and Kass, 1993", "Chen et al., 1996", "Polo-Parada and Korn, 1997", "Alvarez et al., 2000", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytgat et al., 1990", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytg...
76
40,692
0
false
In addition, protons either reduce single channel conductance in 110 mM Ca2+
[]
In addition, protons either reduce single channel conductance in 110 mM Ca2+
true
true
false
true
false
7,017
0
DISCUSSION
1
Tytgat et al., 1990
[ "bib22", "bib33", "bib26", "bib16", "bib6", "bib21", "bib1", "bib8", "bib25", "bib33", "bib8", "bib25", "bib33", "bib8", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
(Tytgat et al., 1990), or increase the whole-cell conductance and shift of channel selectivity toward monovalent ions in nearly physiological conditions (2.5 mM Ca2+ and 140 mM Na+) (Delisle and Satin, 2000).
[ "Prod'hom et al., 1989", "Tytgat et al., 1990", "Shuba et al., 1991", "Kwan and Kass, 1993", "Chen et al., 1996", "Polo-Parada and Korn, 1997", "Alvarez et al., 2000", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytgat et al., 1990", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytg...
208
40,693
1
false
, or increase the whole-cell conductance and shift of channel selectivity toward monovalent ions in nearly physiological conditions (2.5 mM Ca2+ and 140 mM Na+).
[ "Tytgat et al., 1990", "Delisle and Satin, 2000" ]
, or increase the whole-cell conductance and shift of channel selectivity toward monovalent ions in nearly physiological conditions.
false
false
true
true
false
7,017
0
DISCUSSION
1
Prod'hom et al., 1989
[ "bib22", "bib33", "bib26", "bib16", "bib6", "bib21", "bib1", "bib8", "bib25", "bib33", "bib8", "bib25", "bib33", "bib8", "bib16", "bib6", "bib7" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Taking into account that Ca2+ and protons interact in the modulation of channel gating and ion permeation in HVA Ca2+ channels (Kwan and Kass, 1993; Chen et al., 1996; Chen and Tsien, 1997), we were interested to investigate if Ca2+ modulates the effects of pHe on T-type channel function.
[ "Prod'hom et al., 1989", "Tytgat et al., 1990", "Shuba et al., 1991", "Kwan and Kass, 1993", "Chen et al., 1996", "Polo-Parada and Korn, 1997", "Alvarez et al., 2000", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytgat et al., 1990", "Delisle and Satin, 2000", "Shah et al., 2001", "Tytg...
289
40,694
0
false
Taking into account that Ca2+ and protons interact in the modulation of channel gating and ion permeation in HVA Ca2+ channels, we were interested to investigate if Ca2+ modulates the effects of pHe on T-type channel function.
[ "Kwan and Kass, 1993; Chen et al., 1996; Chen and Tsien, 1997" ]
Taking into account that Ca2+ and protons interact in the modulation of channel gating and ion permeation in HVA Ca2+ channels, we were interested to investigate if Ca2+ modulates the effects of pHe on T-type channel function.
true
true
true
true
true
7,017
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
Tytgat et al.
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
14
40,695
0
false
Tytgat et al.
[]
Tytgat et al.
true
true
true
true
true
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
(1990) reported that changes in pHe from 9 to 6 did not shift the voltage for half-maximal activation V act in the presence of 110 mM Ca2+, but significantly changed it in 5.4 mM Ca2+.
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
184
40,696
0
false
(1990) reported that changes in pHe from 9 to 6 did not shift the voltage for half-maximal activation V act in the presence of 110 mM Ca2+, but significantly changed it in 5.4 mM Ca2+.
[]
reported that changes in pHe from 9 to 6 did not shift the voltage for half-maximal activation V act in the presence of 110 mM Ca2+, but significantly changed it in 5.4 mM Ca2+.
false
true
true
true
false
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
In the present study, we show that extracellular acidification induced a shift of V act and increased the slope factor of activation s act in the presence of either 0, 2 or 20 mM Ca2+, and that the magnitudes of these effects were more prominent at lower [Ca2+]e.
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
263
40,697
0
false
In the present study, we show that extracellular acidification induced a shift of V act and increased the slope factor of activation s act in the presence of either 0, 2 or 20 mM Ca2+, and that the magnitudes of these effects were more prominent at lower [Ca2+]e.
[]
In the present study, we show that extracellular acidification induced a shift of V act and increased the slope factor of activation s act in the presence of either 0, 2 or 20 mM Ca2+, and that the magnitudes of these effects were more prominent at lower e.
true
true
true
true
true
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
Remarkably, protons shifted V act to more positive values in Ca2+ free solutions than in 2 or 20 mM Ca2+
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
104
40,698
0
false
Remarkably, protons shifted V act to more positive values in Ca2+ free solutions than in 2 or 20 mM Ca2+
[]
Remarkably, protons shifted V act to more positive values in Ca2+ free solutions than in 2 or 20 mM Ca2+
true
true
false
true
false
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
4), indicating that protons induce an extra positive shift in the voltage dependence of the activation process that cannot be explained by the neutralization of surface charges.
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
177
40,699
0
false
4), indicating that protons induce an extra positive shift in the voltage dependence of the activation process that cannot be explained by the neutralization of surface charges.
[]
4), indicating that protons induce an extra positive shift in the voltage dependence of the activation process that cannot be explained by the neutralization of surface charges.
false
false
true
true
false
7,018
1
DISCUSSION
1
Woodhull, 1973
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
The shift of V act and increase of s act could be interpreted as a voltage-dependent open pore block by protons (Woodhull, 1973).
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
129
40,700
1
false
The shift of V act and increase of s act could be interpreted as a voltage-dependent open pore block by protons.
[ "Woodhull, 1973" ]
The shift of V act and increase of s act could be interpreted as a voltage-dependent open pore block by protons.
true
true
true
true
true
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
However, the finding that Ca2+-dependent proton effects on the voltage dependence of τact were similar to those on steady-state activation indicate that the proton effects on the shape of the current-voltage relationship are to a large extent due to modifications in channel gating.
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
282
40,701
0
false
However, the finding that Ca2+-dependent proton effects on the voltage dependence of τact were similar to those on steady-state activation indicate that the proton effects on the shape of the current-voltage relationship are to a large extent due to modifications in channel gating.
[]
However, the finding that Ca2+-dependent proton effects on the voltage dependence of τact were similar to those on steady-state activation indicate that the proton effects on the shape of the current-voltage relationship are to a large extent due to modifications in channel gating.
true
true
true
true
true
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
Moreover, the open pore block by protons was proven to be voltage independent when NMDG+ was present in the bath solution.
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
122
40,702
0
false
Moreover, the open pore block by protons was proven to be voltage independent when NMDG+ was present in the bath solution.
[]
Moreover, the open pore block by protons was proven to be voltage independent when NMDG+ was present in the bath solution.
true
true
true
true
true
7,018
1
DISCUSSION
1
Tytgat et al. (1990)
[ "bib33", "bib35" ]
12,743,167
NA|NA|NA|NA
In a more physiological condition (150 mM extracellular Na+), the voltage dependence of the proton block shows an opposite slope to that expected from a Woodhull-like open pore block (see below).
[ "Tytgat et al. (1990)", "Woodhull, 1973" ]
195
40,703
0
false
In a more physiological condition (150 mM extracellular Na+), the voltage dependence of the proton block shows an opposite slope to that expected from a Woodhull-like open pore block (see below).
[]
In a more physiological condition, the voltage dependence of the proton block shows an opposite slope to that expected from a Woodhull-like open pore block.
true
true
true
true
true
7,018
2
DISCUSSION
1
Tytgat et al., 1990
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
Extracellular protons also affected inactivation of T-type channels by shifting the voltage for half-maximal inactivation V inac, as it has been reported previously for cardiac T-type Ca2+ channels (Tytgat et al., 1990) and the T-type α1H subunit (Delisle and Satin, 2000).
[ "Tytgat et al., 1990", "Delisle and Satin, 2000" ]
273
40,704
1
false
Extracellular protons also affected inactivation of T-type channels by shifting the voltage for half-maximal inactivation V inac, as it has been reported previously for cardiac T-type Ca2+ channels and the T-type α1H subunit.
[ "Tytgat et al., 1990", "Delisle and Satin, 2000" ]
Extracellular protons also affected inactivation of T-type channels by shifting the voltage for half-maximal inactivation V inac, as it has been reported previously for cardiac T-type Ca2+ channels and the T-type α1H subunit.
true
true
true
true
true
7,019
2
DISCUSSION
1
Tytgat et al., 1990
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
Changes in the slope factor of the inactivation curve s inac had not been reported so far.
[ "Tytgat et al., 1990", "Delisle and Satin, 2000" ]
90
40,705
0
false
Changes in the slope factor of the inactivation curve s inac had not been reported so far.
[]
Changes in the slope factor of the inactivation curve s inac had not been reported so far.
true
true
true
true
true
7,019
2
DISCUSSION
1
Tytgat et al., 1990
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
Our results show that extracellular Ca2+ antagonizes the effects of pHe on both V inac and s inac of α1G, in the sense that significant changes in these parameters require larger changes in pHe at higher Ca2+ concentrations.
[ "Tytgat et al., 1990", "Delisle and Satin, 2000" ]
224
40,706
0
false
Our results show that extracellular Ca2+ antagonizes the effects of pHe on both V inac and s inac of α1G, in the sense that significant changes in these parameters require larger changes in pHe at higher Ca2+ concentrations.
[]
Our results show that extracellular Ca2+ antagonizes the effects of pHe on both V inac and s inac of α1G, in the sense that significant changes in these parameters require larger changes in pHe at higher Ca2+ concentrations.
true
true
true
true
true
7,019
2
DISCUSSION
1
Tytgat et al., 1990
[ "bib33", "bib8" ]
12,743,167
NA|NA|NA|NA
These effects of protons on channel inactivation might be indirect, due to a coupling between activation and inactivation, as discussed in the next section.
[ "Tytgat et al., 1990", "Delisle and Satin, 2000" ]
156
40,707
0
false
These effects of protons on channel inactivation might be indirect, due to a coupling between activation and inactivation, as discussed in the next section.
[]
These effects of protons on channel inactivation might be indirect, due to a coupling between activation and inactivation, as discussed in the next section.
true
true
true
true
true
7,019
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Extracellular protons shift the voltage dependence of the time constant of deactivation τdeac of α1G along the voltage axis, in agreement with the results of Delisle and Satin (2000) for α1H.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
191
40,708
0
false
Extracellular protons shift the voltage dependence of the time constant of deactivation τdeac of α1G along the voltage axis, in agreement with the results of Delisle and Satin (2000) for α1H.
[]
Extracellular protons shift the voltage dependence of the time constant of deactivation τdeac of α1G along the voltage axis, in agreement with the results of Delisle and Satin for α1H.
true
true
true
true
true
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Furthermore, we show that this shift, as for the other gating processes, depends on [Ca2+]e.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
92
40,709
0
false
Furthermore, we show that this shift, as for the other gating processes, depends on [Ca2+]e.
[]
Furthermore, we show that this shift, as for the other gating processes, depends on e.
true
true
true
true
true
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Delisle and Satin (2000) found similar depolarizing shifts of the activation and deactivation processes for a pHe change from 8.2 to 5.5 in 2.5 mM Ca2+, and suggested that these might be due to the neutralization of surface charges by extracellular protons.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
257
40,710
0
false
Delisle and Satin (2000) found similar depolarizing shifts of the activation and deactivation processes for a pHe change from 8.2 to 5.5 in 2.5 mM Ca2+, and suggested that these might be due to the neutralization of surface charges by extracellular protons.
[]
Delisle and Satin found similar depolarizing shifts of the activation and deactivation processes for a pHe change from 8.2 to 5.5 in 2.5 mM Ca2+, and suggested that these might be due to the neutralization of surface charges by extracellular protons.
true
true
true
true
true
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
However, a closer examination of the Ca2+ effects reveals peculiar features of the proton modulation.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
101
40,711
0
false
However, a closer examination of the Ca2+ effects reveals peculiar features of the proton modulation.
[]
However, a closer examination of the Ca2+ effects reveals peculiar features of the proton modulation.
true
true
true
true
true
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
With a milder acidification we observed that the proton-induced shift in Vτdeac was larger than that in V act at high [Ca2+]e.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
126
40,712
0
false
With a milder acidification we observed that the proton-induced shift in Vτdeac was larger than that in V act at high [Ca2+]e.
[]
With a milder acidification we observed that the proton-induced shift in Vτdeac was larger than that in V act at high e.
true
true
true
true
true
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Extracellular acidification from pH 9.1 to 6.2 shifted Vτdeac and V act, respectively, by 19 and 20.1 mV in 2 mM Ca2+, but by 13 and 7 mV in 20 mM Ca2+ (compare Figs.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
166
40,713
0
false
Extracellular acidification from pH 9.1 to 6.2 shifted Vτdeac and V act, respectively, by 19 and 20.1 mV in 2 mM Ca2+, but by 13 and 7 mV in 20 mM Ca2+ (compare Figs.
[]
Extracellular acidification from pH 9.1 to 6.2 shifted Vτdeac and V act, respectively, by 19 and 20.1 mV in 2 mM Ca2+, but by 13 and 7 mV in 20 mM Ca2+ (compare Figs.
true
true
true
true
true
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
4 B and 5 C with Fig.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
21
40,714
0
false
4 B and 5 C with Fig.
[]
4 B and 5 C with Fig.
false
false
true
true
false
7,020
3
DISCUSSION
1
Delisle and Satin (2000)
[ "bib8", "bib8", "bib8", "bib11", "bib12" ]
12,743,167
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
We believe that these results are not in contradiction with those of Delisle and Satin (2000) because they worked in proton-favoring conditions that possibly override Ca2+ effects.
[ "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Delisle and Satin (2000)", "Frankenhaeuser and Hodgkin, 1957", "Hille, 2001" ]
180
40,715
0
false
We believe that these results are not in contradiction with those of Delisle and Satin (2000) because they worked in proton-favoring conditions that possibly override Ca2+ effects.
[]
We believe that these results are not in contradiction with those of Delisle and Satin because they worked in proton-favoring conditions that possibly override Ca2+ effects.
true
true
true
true
true
7,020