Datasets:
vgainullin
/
xciting_data

Dataset card Data Studio Files
xet
 Community
1
paragraph_index
int64
sec
string
p_has_citation
int64
cites
string
citeids
list
pmid
int64
cited_id
string
sentences
string
all_sent_cites
list
sent_len
int64
sentence_batch_index
int64
sent_has_citation
float64
qc_fail
bool
cited_sentence
string
cites_in_sentence
list
cln_sentence
string
is_cap
bool
is_alpha
bool
ends_wp
bool
cit_qc
bool
lgtm
bool
__index_level_0__
int64
2
DISCUSSION
1
61
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
Until more is known about this process, the evidentiary strength of indels will be open to question, and such characters would be better used not as absolute evidence as in the work of Gupta et al.
[ "61", "16", "17", "36" ]
197
8,500
0
false
Until more is known about this process, the evidentiary strength of indels will be open to question, and such characters would be better used not as absolute evidence as in the work of Gupta et al.
[]
Until more is known about this process, the evidentiary strength of indels will be open to question, and such characters would be better used not as absolute evidence as in the work of Gupta et al.
true
true
true
true
true
1,366
2
DISCUSSION
1
61
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
but simply as relative evidence to decide among competing hypotheses.
[ "61", "16", "17", "36" ]
69
8,501
0
false
but simply as relative evidence to decide among competing hypotheses.
[]
but simply as relative evidence to decide among competing hypotheses.
false
true
true
true
false
1,366
2
DISCUSSION
1
61
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
Another type of discrete character—gene order—has been judged relatively weak for deep bacterial phylogeny (61), yet valuable for eukaryotic phylogeny based on mitochondrial genomes (16,17).
[ "61", "16", "17", "36" ]
190
8,502
1
false
Another type of discrete character—gene order—has been judged relatively weak for deep bacterial phylogeny, yet valuable for eukaryotic phylogeny based on mitochondrial genomes.
[ "61", "16,17" ]
Another type of discrete character—gene order—has been judged relatively weak for deep bacterial phylogeny, yet valuable for eukaryotic phylogeny based on mitochondrial genomes.
true
true
true
true
true
1,366
2
DISCUSSION
1
61
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
Other basic questions also remain to be explored.
[ "61", "16", "17", "36" ]
49
8,503
0
false
Other basic questions also remain to be explored.
[]
Other basic questions also remain to be explored.
true
true
true
true
true
1,366
2
DISCUSSION
1
61
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
Is our classification of synapomorphy types adequate, or would a more detailed and exhaustive classification be useful?
[ "61", "16", "17", "36" ]
119
8,504
0
false
Is our classification of synapomorphy types adequate, or would a more detailed and exhaustive classification be useful?
[]
Is our classification of synapomorphy types adequate, or would a more detailed and exhaustive classification be useful?
true
true
true
true
true
1,366
2
DISCUSSION
1
61
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
Will the discovery of new molecular synapomorphies elucidate epochal events [e.g.
[ "61", "16", "17", "36" ]
81
8,505
0
false
Will the discovery of new molecular synapomorphies elucidate epochal events [e.g.
[]
Will the discovery of new molecular synapomorphies elucidate epochal events [e.g.
true
true
true
true
true
1,366
2
DISCUSSION
1
36
[ "b61", "b16", "b17", "b36" ]
16,936,320
pmid-7984417|pmid-15034147|pmid-8783936|pmid-10101183|pmid-7603565|pmid-15535883
colonization of land (36)] in the history of prokaryotic life?
[ "61", "16", "17", "36" ]
62
8,506
1
false
colonization of land ] in the history of prokaryotic life?
[ "36" ]
colonization of land ] in the history of prokaryotic life?
false
true
true
true
false
1,366
0
INTRODUCTION
1
1
[ "B1", "B2", "B3" ]
17,341,465
pmid-16408028|pmid-1340131|pmid-15267220
Chemical modification of nucleic acids is being studied extensively as an approach for the development of nucleic acid-based therapies, such as antigene, antisense and small interfering RNA (siRNA) methods and for applications in biotechnology.
[ "1", "2", "3" ]
244
8,507
0
false
Chemical modification of nucleic acids is being studied extensively as an approach for the development of nucleic acid-based therapies, such as antigene, antisense and small interfering RNA (siRNA) methods and for applications in biotechnology.
[]
Chemical modification of nucleic acids is being studied extensively as an approach for the development of nucleic acid-based therapies, such as antigene, antisense and small interfering RNA (siRNA) methods and for applications in biotechnology.
true
true
true
true
true
1,367
0
INTRODUCTION
1
1
[ "B1", "B2", "B3" ]
17,341,465
pmid-16408028|pmid-1340131|pmid-15267220
In the case of the antigene method, oligonucleotides are aimed at DNA molecules in the single-stranded (1) or double-stranded state (2) to block gene transcription.
[ "1", "2", "3" ]
164
8,508
1
false
In the case of the antigene method, oligonucleotides are aimed at DNA molecules in the single-stranded or double-stranded state to block gene transcription.
[ "1", "2" ]
In the case of the antigene method, oligonucleotides are aimed at DNA molecules in the single-stranded or double-stranded state to block gene transcription.
true
true
true
true
true
1,367
0
INTRODUCTION
1
3
[ "B1", "B2", "B3" ]
17,341,465
pmid-16408028|pmid-1340131|pmid-15267220
On the other hand, the goal with antisense oligonucleotides is to form a hybrid duplex with the mRNA, thereby inhibiting gene expression at the level of translation (3).
[ "1", "2", "3" ]
169
8,509
1
false
On the other hand, the goal with antisense oligonucleotides is to form a hybrid duplex with the mRNA, thereby inhibiting gene expression at the level of translation.
[ "3" ]
On the other hand, the goal with antisense oligonucleotides is to form a hybrid duplex with the mRNA, thereby inhibiting gene expression at the level of translation.
true
true
true
true
true
1,367
0
INTRODUCTION
1
1
[ "B1", "B2", "B3" ]
17,341,465
pmid-16408028|pmid-1340131|pmid-15267220
Regardless of the target nucleic acids and the levels at which they inhibit gene expression, it is desirable for these putative oligonucleotide therapies to possess high target binding affinity and specificity, resistance against nuclease degradation and cell permeability.
[ "1", "2", "3" ]
273
8,510
0
false
Regardless of the target nucleic acids and the levels at which they inhibit gene expression, it is desirable for these putative oligonucleotide therapies to possess high target binding affinity and specificity, resistance against nuclease degradation and cell permeability.
[]
Regardless of the target nucleic acids and the levels at which they inhibit gene expression, it is desirable for these putative oligonucleotide therapies to possess high target binding affinity and specificity, resistance against nuclease degradation and cell permeability.
true
true
true
true
true
1,367
0
INTRODUCTION
1
1
[ "B1", "B2", "B3" ]
17,341,465
pmid-16408028|pmid-1340131|pmid-15267220
The efficacy of antisense oligonucleotides can be enhanced by activating RNase H to cleave only the RNA strand of the RNA:DNA hybrid duplex.
[ "1", "2", "3" ]
140
8,511
0
false
The efficacy of antisense oligonucleotides can be enhanced by activating RNase H to cleave only the RNA strand of the RNA:DNA hybrid duplex.
[]
The efficacy of antisense oligonucleotides can be enhanced by activating RNase H to cleave only the RNA strand of the RNA:DNA hybrid duplex.
true
true
true
true
true
1,367
1
INTRODUCTION
1
12
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
In view of these, a wide variety of oligonucleotides containing modifications at the nucleobase (4–11), the sugar ring or the phosphodiester backbone have been introduced (12).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
176
8,512
1
false
In view of these, a wide variety of oligonucleotides containing modifications at the nucleobase, the sugar ring or the phosphodiester backbone have been introduced.
[ "4–11", "12" ]
In view of these, a wide variety of oligonucleotides containing modifications at the nucleobase, the sugar ring or the phosphodiester backbone have been introduced.
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
The most common phosphodiester-modified oligonucleotide is phosphorothioate DNA (PS DNA) in which one of the non-bridging phosphate oxygen atoms is replaced with sulfur.
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
169
8,513
0
false
The most common phosphodiester-modified oligonucleotide is phosphorothioate DNA (PS DNA) in which one of the non-bridging phosphate oxygen atoms is replaced with sulfur.
[]
The most common phosphodiester-modified oligonucleotide is phosphorothioate DNA (PS DNA) in which one of the non-bridging phosphate oxygen atoms is replaced with sulfur.
true
true
true
true
true
1,368
1
INTRODUCTION
1
13
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
PS DNAs are easy to synthesize, and have increased nuclease resistance compared to unmodified DNA oligonucleotides (13).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
120
8,514
1
false
PS DNAs are easy to synthesize, and have increased nuclease resistance compared to unmodified DNA oligonucleotides.
[ "13" ]
PS DNAs are easy to synthesize, and have increased nuclease resistance compared to unmodified DNA oligonucleotides.
true
true
true
true
true
1,368
1
INTRODUCTION
1
14
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
However, there are also some disadvantages of PS DNAs, such as low binding affinity for RNA targets (14) and non-sequence-specific activity (15,16).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
148
8,515
1
false
However, there are also some disadvantages of PS DNAs, such as low binding affinity for RNA targets and non-sequence-specific activity.
[ "14", "15,16" ]
However, there are also some disadvantages of PS DNAs, such as low binding affinity for RNA targets and non-sequence-specific activity.
true
true
true
true
true
1,368
1
INTRODUCTION
1
17
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
On the contrary, polyamines are known to have high binding affinity for DNA and to increase duplex (17) and triplex stabilities (18).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
133
8,516
1
false
On the contrary, polyamines are known to have high binding affinity for DNA and to increase duplex and triplex stabilities.
[ "17", "18" ]
On the contrary, polyamines are known to have high binding affinity for DNA and to increase duplex and triplex stabilities.
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
Thus, we synthesized oligonucleotides carrying various polyamines at the nucleobases (19–23) or at the sugar rings (24–26).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
123
8,517
0
false
Thus, we synthesized oligonucleotides carrying various polyamines at the nucleobases or at the sugar rings.
[ "19–23", "24–26" ]
Thus, we synthesized oligonucleotides carrying various polyamines at the nucleobases or at the sugar rings.
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
Some of them stabilized duplexes and triplexes and enhanced nuclease resistance.
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
80
8,518
0
false
Some of them stabilized duplexes and triplexes and enhanced nuclease resistance.
[]
Some of them stabilized duplexes and triplexes and enhanced nuclease resistance.
true
true
true
true
true
1,368
1
INTRODUCTION
1
20
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
In particular, incorporation of 5-(N-aminohexyl)carbamoyl-2′-deoxyuridine (NU; Figure 1a) into DNA strands increased the thermal stability of the corresponding DNA:DNA duplexes by ∼4.3°C (20) (see Figure 1c).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
208
8,519
1
false
In particular, incorporation of 5-(N-aminohexyl)carbamoyl-2′-deoxyuridine (NU; Figure 1a) into DNA strands increased the thermal stability of the corresponding DNA:DNA duplexes by ∼4.3°C (see Figure 1c).
[ "20" ]
In particular, incorporation of 5-(N-aminohexyl)carbamoyl-2′-deoxyuridine (NU; Figure 1a) into DNA strands increased the thermal stability of the corresponding DNA:DNA duplexes by ∼4.3°C (see Figure 1c).
true
true
true
true
true
1,368
1
INTRODUCTION
1
23
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
Since 2′-modifications are known to increase binding affinity for RNA and to improve nuclease resistance (27), we also synthesized oligonucleotides containing 5-(N-aminohexyl)carbamoyl-2′-O-methyluridine (23) (NUm in Figure 1a).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
228
8,520
1
false
Since 2′-modifications are known to increase binding affinity for RNA and to improve nuclease resistance, we also synthesized oligonucleotides containing 5-(N-aminohexyl)carbamoyl-2′-O-methyluridine (NUm in Figure 1a).
[ "27", "23" ]
Since 2′-modifications are known to increase binding affinity for RNA and to improve nuclease resistance, we also synthesized oligonucleotides containing 5-(N-aminohexyl)carbamoyl-2′-O-methyluridine (NUm in Figure 1a).
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
Such oligonucleotides formed stable hybrid duplexes with complementary RNAs, with an increase of ∼3.9°C relative to their unmodified DNA:RNA counterparts.
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
154
8,521
0
false
Such oligonucleotides formed stable hybrid duplexes with complementary RNAs, with an increase of ∼3.9°C relative to their unmodified DNA:RNA counterparts.
[]
Such oligonucleotides formed stable hybrid duplexes with complementary RNAs, with an increase of ∼3.9°C relative to their unmodified DNA:RNA counterparts.
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
They also showed slightly higher binding affinities for RNA compared to the oligonucleotides containing NU.
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
107
8,522
0
false
They also showed slightly higher binding affinities for RNA compared to the oligonucleotides containing NU.
[]
They also showed slightly higher binding affinities for RNA compared to the oligonucleotides containing NU.
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
However, NUm residues decreased binding affinity for DNA (Figure 1c).
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
69
8,523
0
false
However, NUm residues decreased binding affinity for DNA (Figure 1c).
[]
However, NUm residues decreased binding affinity for DNA (Figure 1c).
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
Interestingly, the oligonucleotides containing NUm further exceed the improved resistance of those containing NU against degradation by both exo- and endo-nucleases.
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
165
8,524
0
false
Interestingly, the oligonucleotides containing NUm further exceed the improved resistance of those containing NU against degradation by both exo- and endo-nucleases.
[]
Interestingly, the oligonucleotides containing NUm further exceed the improved resistance of those containing NU against degradation by both exo- and endo-nucleases.
true
true
true
true
true
1,368
1
INTRODUCTION
1
4
[ "B4", "B11", "B12", "B13", "B14", "B15", "B16", "B17", "B18", "B19", "B23", "B24", "B26", "B20", "B27", "B23" ]
17,341,465
pmid-10600379|pmid-12062382|pmid-9358149|pmid-2411211|pmid-1711677|pmid-7852327|pmid-9177251|pmid-13918835|pmid-2021635|NA|pmid-12736300|pmid-8305519|pmid-11087344|pmid-9177831|pmid-7688569|pmid-12736300
The hybrid duplexes formed between DNA strands containing NUm and RNA could act as substrates for RNase H, on the condition that the NUm residues are separated by at least five continuous unmodified 2′-deoxyribonucleotides.
[ "4", "11", "12", "13", "14", "15", "16", "17", "18", "19", "23", "24", "26", "20", "27", "23" ]
223
8,525
0
false
The hybrid duplexes formed between DNA strands containing NUm and RNA could act as substrates for RNase H, on the condition that the NUm residues are separated by at least five continuous unmodified 2′-deoxyribonucleotides.
[]
The hybrid duplexes formed between DNA strands containing NUm and RNA could act as substrates for RNase H, on the condition that the NUm residues are separated by at least five continuous unmodified 2′-deoxyribonucleotides.
true
true
true
true
true
1,368
2
INTRODUCTION
1
23
[ "B23" ]
17,341,465
pmid-12736300
Structures of the modified nucleoside analogs (a), sequences and numbering schemes (b), and thermal denaturation of the DNA:DNA and DNA:RNA duplexes (c).
[ "23" ]
153
8,526
0
false
Structures of the modified nucleoside analogs (a), sequences and numbering schemes (b), and thermal denaturation of the DNA:DNA and DNA:RNA duplexes (c).
[]
Structures of the modified nucleoside analogs (a), sequences and numbering schemes (b), and thermal denaturation of the DNA:DNA and DNA:RNA duplexes (c).
true
true
true
true
true
1,369
2
INTRODUCTION
1
23
[ "B23" ]
17,341,465
pmid-12736300
The thermal denaturation of the duplexes was performed as described in (23).
[ "23" ]
76
8,527
1
false
The thermal denaturation of the duplexes was performed as described in.
[ "23" ]
The thermal denaturation of the duplexes was performed as described in.
true
true
true
true
true
1,369
3
INTRODUCTION
0
null
null
17,341,465
null
In this paper, we report the X-ray crystal structures of B-form DNA:DNA duplexes containing either NU or NUm, and of an A-form DNA:RNA hybrid duplex containing NUm.
null
164
8,528
0
false
null
null
In this paper, we report the X-ray crystal structures of B-form DNA:DNA duplexes containing either NU or NUm, and of an A-form DNA:RNA hybrid duplex containing NUm.
true
true
true
true
true
1,370
3
INTRODUCTION
0
null
null
17,341,465
null
Based on these duplex structures, we analyzed the correlations between the conformational changes brought about by the NU and NUm residues and the desirable therapeutic properties, such as target binding affinity and nuclease resistance.
null
237
8,529
0
false
null
null
Based on these duplex structures, we analyzed the correlations between the conformational changes brought about by the NU and NUm residues and the desirable therapeutic properties, such as target binding affinity and nuclease resistance.
true
true
true
true
true
1,370
3
INTRODUCTION
0
null
null
17,341,465
null
In addition, we determined the effects of the NU and NUm residues on the minor groove dimensions and hydration structures, since such parameters are expected to affect recognition by nucleases and RNase H.
null
205
8,530
0
false
null
null
In addition, we determined the effects of the NU and NUm residues on the minor groove dimensions and hydration structures, since such parameters are expected to affect recognition by nucleases and RNase H.
true
true
true
true
true
1,370
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
DNA replication in eukaryotes is initiated through the coordinated actions of replication initiation proteins.
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
110
8,531
0
false
DNA replication in eukaryotes is initiated through the coordinated actions of replication initiation proteins.
[]
DNA replication in eukaryotes is initiated through the coordinated actions of replication initiation proteins.
true
true
true
true
true
1,371
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
Studies in yeast and other species have identified many initiation proteins and revealed the outline of the mechanism through which DNA replication begins in eukaryotic cells (1–3).
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
181
8,532
0
false
Studies in yeast and other species have identified many initiation proteins and revealed the outline of the mechanism through which DNA replication begins in eukaryotic cells.
[ "1–3" ]
Studies in yeast and other species have identified many initiation proteins and revealed the outline of the mechanism through which DNA replication begins in eukaryotic cells.
true
true
true
true
true
1,371
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
In brief, an origin recognition complex (ORC) consisting of the ORC1–6 subunits binds to DNA and then, in cooperation with CDC6 and CDT1, loads a mini-chromosome maintenance (MCM) 2–7 protein complex on to the DNA to form a specific protein–DNA complex, which is called the pre-replicative complex (pre-RC).
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
307
8,533
0
false
In brief, an origin recognition complex (ORC) consisting of the ORC1–6 subunits binds to DNA and then, in cooperation with CDC6 and CDT1, loads a mini-chromosome maintenance (MCM) 2–7 protein complex on to the DNA to form a specific protein–DNA complex, which is called the pre-replicative complex (pre-RC).
[]
In brief, an origin recognition complex (ORC) consisting of the ORC1–6 subunits binds to DNA and then, in cooperation with CDC6 and CDT1, loads a mini-chromosome maintenance (MCM) 2–7 protein complex on to the DNA to form a specific protein–DNA complex, which is called the pre-replicative complex (pre-RC).
true
true
true
true
true
1,371
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
MCM2–7 is thought to function as a DNA helicase during the initiation and elongation steps (4–9).
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
97
8,534
0
false
MCM2–7 is thought to function as a DNA helicase during the initiation and elongation steps.
[ "4–9" ]
MCM2–7 is thought to function as a DNA helicase during the initiation and elongation steps.
true
true
true
true
true
1,371
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
Geminin is known to inhibit pre-RC formation during S, G2 and M phases of the cell cycle and thus is considered as the major regulator of pre-RC formation during the cell cycle (2,10).
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
184
8,535
0
false
Geminin is known to inhibit pre-RC formation during S, G2 and M phases of the cell cycle and thus is considered as the major regulator of pre-RC formation during the cell cycle.
[ "2,10" ]
Geminin is known to inhibit pre-RC formation during S, G2 and M phases of the cell cycle and thus is considered as the major regulator of pre-RC formation during the cell cycle.
true
true
true
true
true
1,371
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
However, although the basic mechanism of pre-RC formation is understood, many details remain to be defined.
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
107
8,536
0
false
However, although the basic mechanism of pre-RC formation is understood, many details remain to be defined.
[]
However, although the basic mechanism of pre-RC formation is understood, many details remain to be defined.
true
true
true
true
true
1,371
0
INTRODUCTION
1
1
[ "b1", "b3", "b4", "b9", "b2", "b10", "b1", "b11", "b17" ]
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
After formation, the pre-RC is activated in a poorly understood process that involves many factors, including cyclin-dependent kinase (CDK) and CDC7 kinase, and activation leads to replication fork formation (1,11–17).
[ "1", "3", "4", "9", "2", "10", "1", "11", "17" ]
218
8,537
0
false
After formation, the pre-RC is activated in a poorly understood process that involves many factors, including cyclin-dependent kinase (CDK) and CDC7 kinase, and activation leads to replication fork formation.
[ "1,11–17" ]
After formation, the pre-RC is activated in a poorly understood process that involves many factors, including cyclin-dependent kinase (CDK) and CDC7 kinase, and activation leads to replication fork formation.
true
true
true
true
true
1,371
1
INTRODUCTION
1
18
[ "b18", "b20", "b21", "b19", "b18" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
To study the details of pre-RC formation and activation, a biochemical approach using in vitro analysis of the pre-RC is needed.
[ "18", "20", "21", "19", "18" ]
128
8,538
0
false
To study the details of pre-RC formation and activation, a biochemical approach using in vitro analysis of the pre-RC is needed.
[]
To study the details of pre-RC formation and activation, a biochemical approach using in vitro analysis of the pre-RC is needed.
true
true
true
true
true
1,372
1
INTRODUCTION
1
18
[ "b18", "b20", "b21", "b19", "b18" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
Several studies have taken such in vitro approaches, mainly through analysis of the pre-RC in budding yeast (18–20).
[ "18", "20", "21", "19", "18" ]
116
8,539
0
false
Several studies have taken such in vitro approaches, mainly through analysis of the pre-RC in budding yeast.
[ "18–20" ]
Several studies have taken such in vitro approaches, mainly through analysis of the pre-RC in budding yeast.
true
true
true
true
true
1,372
1
INTRODUCTION
1
21
[ "b18", "b20", "b21", "b19", "b18" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
This approach takes advantage of a property unique to budding yeast among the various species characterized to date: that the ORC binds to a well-characterized, defined replication origin, the so-called autonomously replicating sequence (ARS) (21).
[ "18", "20", "21", "19", "18" ]
248
8,540
1
false
This approach takes advantage of a property unique to budding yeast among the various species characterized to date: that the ORC binds to a well-characterized, defined replication origin, the so-called autonomously replicating sequence (ARS).
[ "21" ]
This approach takes advantage of a property unique to budding yeast among the various species characterized to date: that the ORC binds to a well-characterized, defined replication origin, the so-called autonomously replicating sequence (ARS).
true
true
true
true
true
1,372
1
INTRODUCTION
1
19
[ "b18", "b20", "b21", "b19", "b18" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
Among these studies, the experimental system reported by Seki and Diffley (19) appears to be especially useful for analysis of the pre-RC.
[ "18", "20", "21", "19", "18" ]
138
8,541
1
false
Among these studies, the experimental system reported by Seki and Diffley appears to be especially useful for analysis of the pre-RC.
[ "19" ]
Among these studies, the experimental system reported by Seki and Diffley appears to be especially useful for analysis of the pre-RC.
true
true
true
true
true
1,372
1
INTRODUCTION
1
18
[ "b18", "b20", "b21", "b19", "b18" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
In this system, the pre-RC can be successfully reconstituted on an ARS sequence coupled to paramagnetic beads, and the system can then be used to address the role of ATP hydrolysis by the yeast ORC in pre-RC formation (18).
[ "18", "20", "21", "19", "18" ]
223
8,542
1
false
In this system, the pre-RC can be successfully reconstituted on an ARS sequence coupled to paramagnetic beads, and the system can then be used to address the role of ATP hydrolysis by the yeast ORC in pre-RC formation.
[ "18" ]
In this system, the pre-RC can be successfully reconstituted on an ARS sequence coupled to paramagnetic beads, and the system can then be used to address the role of ATP hydrolysis by the yeast ORC in pre-RC formation.
true
true
true
true
true
1,372
2
INTRODUCTION
1
22
[ "b22", "b23", "b24", "b28", "b29", "b30", "b29", "b31", "b35", "b36" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
Although in vitro replication using yeast nuclear extracts has been reported (22,23), the exact activity of the reconstituted pre-RC in the above studies is unknown.
[ "22", "23", "24", "28", "29", "30", "29", "31", "35", "36" ]
165
8,543
0
false
Although in vitro replication using yeast nuclear extracts has been reported, the exact activity of the reconstituted pre-RC in the above studies is unknown.
[ "22,23" ]
Although in vitro replication using yeast nuclear extracts has been reported, the exact activity of the reconstituted pre-RC in the above studies is unknown.
true
true
true
true
true
1,373
2
INTRODUCTION
1
22
[ "b22", "b23", "b24", "b28", "b29", "b30", "b29", "b31", "b35", "b36" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
In contrast, the Xenopus replication system is able to replicate various kinds of DNA efficiently, whereas initiation generally occurs randomly with respect to the DNA sequence (24–28).
[ "22", "23", "24", "28", "29", "30", "29", "31", "35", "36" ]
185
8,544
0
false
In contrast, the Xenopus replication system is able to replicate various kinds of DNA efficiently, whereas initiation generally occurs randomly with respect to the DNA sequence.
[ "24–28" ]
In contrast, the Xenopus replication system is able to replicate various kinds of DNA efficiently, whereas initiation generally occurs randomly with respect to the DNA sequence.
true
true
true
true
true
1,373
2
INTRODUCTION
1
29
[ "b22", "b23", "b24", "b28", "b29", "b30", "b29", "b31", "b35", "b36" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
However, recent studies have established the conditions for initiation at a specific DNA region, using methylated DNA (29) or transcription factor-bound DNA (30), with one such study showing that MCM loaded on to DNA is distributed over a large region distant from the ORC (29).
[ "22", "23", "24", "28", "29", "30", "29", "31", "35", "36" ]
278
8,545
2
true
However, recent studies have established the conditions for initiation at a specific DNA region, using methylated DNA or transcription factor-bound DNA, with one such study showing that MCM loaded on to DNA is distributed over a large region distant from the ORC.
[ "29", "30", "29" ]
However, recent studies have established the conditions for initiation at a specific DNA region, using methylated DNA or transcription factor-bound DNA, with one such study showing that MCM loaded on to DNA is distributed over a large region distant from the ORC.
true
true
true
true
true
1,373
2
INTRODUCTION
1
22
[ "b22", "b23", "b24", "b28", "b29", "b30", "b29", "b31", "b35", "b36" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
In addition to free plasmids, DNA synthesis on DNA-coupled beads has also been performed with cell extracts of Xenopus eggs, and used to analyze activities such as pre-RC formation, chromatin assembly and spindle assembly (31–35).
[ "22", "23", "24", "28", "29", "30", "29", "31", "35", "36" ]
230
8,546
0
false
In addition to free plasmids, DNA synthesis on DNA-coupled beads has also been performed with cell extracts of Xenopus eggs, and used to analyze activities such as pre-RC formation, chromatin assembly and spindle assembly.
[ "31–35" ]
In addition to free plasmids, DNA synthesis on DNA-coupled beads has also been performed with cell extracts of Xenopus eggs, and used to analyze activities such as pre-RC formation, chromatin assembly and spindle assembly.
true
true
true
true
true
1,373
2
INTRODUCTION
1
36
[ "b22", "b23", "b24", "b28", "b29", "b30", "b29", "b31", "b35", "b36" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
Using circular plasmid-coupled magnetic beads, we recently showed that only one or a few MCM complexes may be loaded per ORC DNA-binding event (36), but DNA synthesis on the beads in the egg extracts has not been fully characterized.
[ "22", "23", "24", "28", "29", "30", "29", "31", "35", "36" ]
233
8,547
1
false
Using circular plasmid-coupled magnetic beads, we recently showed that only one or a few MCM complexes may be loaded per ORC DNA-binding event, but DNA synthesis on the beads in the egg extracts has not been fully characterized.
[ "36" ]
Using circular plasmid-coupled magnetic beads, we recently showed that only one or a few MCM complexes may be loaded per ORC DNA-binding event, but DNA synthesis on the beads in the egg extracts has not been fully characterized.
true
true
true
true
true
1,373
3
INTRODUCTION
0
null
null
16,870,720
pmid-9660936
Here, we report the characterization of DNA replication on plasmid-coupled magnetic beads in Xenopus egg extracts.
null
114
8,548
0
false
null
null
Here, we report the characterization of DNA replication on plasmid-coupled magnetic beads in Xenopus egg extracts.
true
true
true
true
true
1,374
3
INTRODUCTION
0
null
null
16,870,720
pmid-9660936
Our data show that DNA replication occurs in a pre-RC- and CDK-dependent manner when a circular plasmid is coupled to magnetic beads and then incubated in Xenopus egg extracts.
null
176
8,549
0
false
null
null
Our data show that DNA replication occurs in a pre-RC- and CDK-dependent manner when a circular plasmid is coupled to magnetic beads and then incubated in Xenopus egg extracts.
true
true
true
true
true
1,374
3
INTRODUCTION
0
null
null
16,870,720
pmid-9660936
Furthermore, we were able to detect regulated DNA binding of replication fork proteins, including three different DNA polymerases, suggesting that bona fide replication forks are formed de novo on the circular plasmid.
null
218
8,550
0
false
null
null
Furthermore, we were able to detect regulated DNA binding of replication fork proteins, including three different DNA polymerases, suggesting that bona fide replication forks are formed de novo on the circular plasmid.
true
true
true
true
true
1,374
3
INTRODUCTION
0
null
null
16,870,720
pmid-9660936
Therefore, this DNA replication model based on plasmid immobilization on beads is likely to be useful for the analysis of pre-RC activation.
null
140
8,551
0
false
null
null
Therefore, this DNA replication model based on plasmid immobilization on beads is likely to be useful for the analysis of pre-RC activation.
true
true
true
true
true
1,374
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
The data in this study show that DNA synthesis on linear plasmid-coupled beads takes place in egg extracts, even under conditions where loading of MCM proteins on to DNA is inhibited.
null
183
8,552
0
false
null
null
The data in this study show that DNA synthesis on linear plasmid-coupled beads takes place in egg extracts, even under conditions where loading of MCM proteins on to DNA is inhibited.
true
true
true
true
true
1,375
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
Although it cannot be formally excluded that MCM proteins may still be involved in DNA synthesis on linear DNA, it seems likely that this synthesis is mediated by other nuclear activities, such as DNA repair.
null
208
8,553
0
false
null
null
Although it cannot be formally excluded that MCM proteins may still be involved in DNA synthesis on linear DNA, it seems likely that this synthesis is mediated by other nuclear activities, such as DNA repair.
true
true
true
true
true
1,375
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
In contrast with the data for linearized plasmids, several lines of evidence suggest that genuine DNA replication takes place on immobilized circular plasmids in the egg extracts.
null
179
8,554
0
false
null
null
In contrast with the data for linearized plasmids, several lines of evidence suggest that genuine DNA replication takes place on immobilized circular plasmids in the egg extracts.
true
true
true
true
true
1,375
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
First, MCM3 and MCM6, probably as the MCM2–7 protein complex, were loaded on to plasmid beads in an ORC-dependent manner, and this MCM loading was inhibited by geminin.
null
168
8,555
0
false
null
null
First, MCM3 and MCM6, probably as the MCM2–7 protein complex, were loaded on to plasmid beads in an ORC-dependent manner, and this MCM loading was inhibited by geminin.
true
true
true
true
true
1,375
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
Second, DNA synthesis occurred in a semi-conservative manner and was also inhibited by geminin and p21, indicating that pre-RC formation and CDK activity-dependent DNA replication occurs.
null
187
8,556
0
false
null
null
Second, DNA synthesis occurred in a semi-conservative manner and was also inhibited by geminin and p21, indicating that pre-RC formation and CDK activity-dependent DNA replication occurs.
true
true
true
true
true
1,375
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
Third, the start of DNA synthesis correlated with the DNA binding of replication fork proteins such as CDC45, RPA, DNA polymerases and PCNA, and binding of these proteins was inhibited by p21.
null
192
8,557
0
false
null
null
Third, the start of DNA synthesis correlated with the DNA binding of replication fork proteins such as CDC45, RPA, DNA polymerases and PCNA, and binding of these proteins was inhibited by p21.
true
true
true
true
true
1,375
0
DISCUSSION
0
null
null
16,870,720
pmid-12045100|pmid-15680967|pmid-15329670|pmid-11741544|pmid-15928711|pmid-9635433|pmid-12045100|pmid-11807498|pmid-12768207
Finally, both binding of replication fork proteins and DNA synthesis were dependent on the formation of a functional nuclear structure.
null
135
8,558
0
false
null
null
Finally, both binding of replication fork proteins and DNA synthesis were dependent on the formation of a functional nuclear structure.
true
true
true
true
true
1,375
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
Although the pre-RC can be formed efficiently on circular plasmid-coupled beads [as shown herein, and also see Ref.
[ "36" ]
115
8,559
0
false
Although the pre-RC can be formed efficiently on circular plasmid-coupled beads [as shown herein, and also see Ref.
[]
Although the pre-RC can be formed efficiently on circular plasmid-coupled beads [as shown herein, and also see Ref.
true
true
true
true
true
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
(36)], it is quite possible that the biotin–streptavidin link between the beads and the DNA affects certain processes during DNA replication, particularly in the elongation step.
[ "36" ]
178
8,560
1
false
], it is quite possible that the biotin–streptavidin link between the beads and the DNA affects certain processes during DNA replication, particularly in the elongation step.
[ "36" ]
], it is quite possible that the biotin–streptavidin link between the beads and the DNA affects certain processes during DNA replication, particularly in the elongation step.
false
false
true
true
false
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
One can imagine that, if a replication fork encounters this link, it may stall immediately.
[ "36" ]
91
8,561
0
false
One can imagine that, if a replication fork encounters this link, it may stall immediately.
[]
One can imagine that, if a replication fork encounters this link, it may stall immediately.
true
true
true
true
true
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
Nevertheless, a closed circular form of replicated DNA was clearly produced, as shown in Figure 1.
[ "36" ]
98
8,562
0
false
Nevertheless, a closed circular form of replicated DNA was clearly produced, as shown in Figure 1.
[]
Nevertheless, a closed circular form of replicated DNA was clearly produced, as shown in Figure 1.
true
true
true
true
true
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
Although we could not determine the number of biotin–streptavidin links formed on each plasmid, some plasmids may have only one biotin–streptavidin link per plasmid.
[ "36" ]
165
8,563
0
false
Although we could not determine the number of biotin–streptavidin links formed on each plasmid, some plasmids may have only one biotin–streptavidin link per plasmid.
[]
Although we could not determine the number of biotin–streptavidin links formed on each plasmid, some plasmids may have only one biotin–streptavidin link per plasmid.
true
true
true
true
true
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
Under these circumstances, two replication forks could meet each other at the link, thereby allowing the DNA strand that is not directly connected to the bead to be replicated completely, and producing a closed circular daughter molecule.
[ "36" ]
238
8,564
0
false
Under these circumstances, two replication forks could meet each other at the link, thereby allowing the DNA strand that is not directly connected to the bead to be replicated completely, and producing a closed circular daughter molecule.
[]
Under these circumstances, two replication forks could meet each other at the link, thereby allowing the DNA strand that is not directly connected to the bead to be replicated completely, and producing a closed circular daughter molecule.
true
true
true
true
true
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
In contrast, if biotin–streptavidin links exist at multiple sites on one plasmid, replication cannot easily be completed and the plasmid may tend to remain bound to the bead, even after the treatment to induce link breakage.
[ "36" ]
224
8,565
0
false
In contrast, if biotin–streptavidin links exist at multiple sites on one plasmid, replication cannot easily be completed and the plasmid may tend to remain bound to the bead, even after the treatment to induce link breakage.
[]
In contrast, if biotin–streptavidin links exist at multiple sites on one plasmid, replication cannot easily be completed and the plasmid may tend to remain bound to the bead, even after the treatment to induce link breakage.
true
true
true
true
true
1,376
1
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-15610739|pmid-16228006|pmid-1579162|pmid-11121019|pmid-15610739|pmid-16497662
This may explain why it is so difficult to recover DNA from the plasmid beads, particularly after prolonged incubation in the egg extracts.
[ "36" ]
139
8,566
0
false
This may explain why it is so difficult to recover DNA from the plasmid beads, particularly after prolonged incubation in the egg extracts.
[]
This may explain why it is so difficult to recover DNA from the plasmid beads, particularly after prolonged incubation in the egg extracts.
true
true
true
true
true
1,376
2
DISCUSSION
1
43
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
The efficiency of DNA synthesis under the conditions described here is relatively low.
[ "43", "44" ]
86
8,567
0
false
The efficiency of DNA synthesis under the conditions described here is relatively low.
[]
The efficiency of DNA synthesis under the conditions described here is relatively low.
true
true
true
true
true
1,377
2
DISCUSSION
1
43
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
Consistent with this, the levels of MCM proteins loaded on to DNA did not decrease during DNA synthesis (Figure 3B).
[ "43", "44" ]
116
8,568
0
false
Consistent with this, the levels of MCM proteins loaded on to DNA did not decrease during DNA synthesis (Figure 3B).
[]
Consistent with this, the levels of MCM proteins loaded on to DNA did not decrease during DNA synthesis.
true
true
true
true
true
1,377
2
DISCUSSION
1
43
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
However, it should be noted that, in addition to Polα and Polδ, Polɛ binds to plasmid-coupled beads during DNA synthesis (Figure 3B).
[ "43", "44" ]
133
8,569
0
false
However, it should be noted that, in addition to Polα and Polδ, Polɛ binds to plasmid-coupled beads during DNA synthesis (Figure 3B).
[]
However, it should be noted that, in addition to Polα and Polδ, Polɛ binds to plasmid-coupled beads during DNA synthesis.
true
true
true
true
true
1,377
2
DISCUSSION
1
43
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
This implies that Polɛ, as well as Polδ, may be a component of the replication fork and may be required for plasmid replication.
[ "43", "44" ]
128
8,570
0
false
This implies that Polɛ, as well as Polδ, may be a component of the replication fork and may be required for plasmid replication.
[]
This implies that Polɛ, as well as Polδ, may be a component of the replication fork and may be required for plasmid replication.
true
true
true
true
true
1,377
2
DISCUSSION
1
43
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
If so, this plasmid replication system may allow elucidation of the role of Polɛ at the replication fork, which has not been possible to determine in previous studies using in vitro SV40 DNA replication (43).
[ "43", "44" ]
208
8,571
1
false
If so, this plasmid replication system may allow elucidation of the role of Polɛ at the replication fork, which has not been possible to determine in previous studies using in vitro SV40 DNA replication.
[ "43" ]
If so, this plasmid replication system may allow elucidation of the role of Polɛ at the replication fork, which has not been possible to determine in previous studies using in vitro SV40 DNA replication.
true
true
true
true
true
1,377
2
DISCUSSION
1
44
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
Consistent with our results, a recent study has shown enrichment of Polα, Polδ and Polɛ at the replication barrier on a plasmid in Xenopus egg extracts (44).
[ "43", "44" ]
157
8,572
1
false
Consistent with our results, a recent study has shown enrichment of Polα, Polδ and Polɛ at the replication barrier on a plasmid in Xenopus egg extracts.
[ "44" ]
Consistent with our results, a recent study has shown enrichment of Polα, Polδ and Polɛ at the replication barrier on a plasmid in Xenopus egg extracts.
true
true
true
true
true
1,377
2
DISCUSSION
1
43
[ "b43", "b44" ]
16,870,720
pmid-9710536|pmid-9203578|pmid-11149917|pmid-1579437|pmid-12972597|pmid-15247921|pmid-12972597|pmid-8684481|pmid-12087101|pmid-16497662|pmid-8144677|pmid-16483939
Although we have prepared Polɛ-immunodepleted egg extracts and used them in the plasmid replication system, clear and reproducible results are yet to be obtained, because DNA synthesis activity in the extracts decreases dramatically after depletion treatment, even with mock depletion.
[ "43", "44" ]
285
8,573
0
false
Although we have prepared Polɛ-immunodepleted egg extracts and used them in the plasmid replication system, clear and reproducible results are yet to be obtained, because DNA synthesis activity in the extracts decreases dramatically after depletion treatment, even with mock depletion.
[]
Although we have prepared Polɛ-immunodepleted egg extracts and used them in the plasmid replication system, clear and reproducible results are yet to be obtained, because DNA synthesis activity in the extracts decreases dramatically after depletion treatment, even with mock depletion.
true
true
true
true
true
1,377
3
DISCUSSION
1
26
[ "b26" ]
16,870,720
pmid-9660936
A nuclear-free Xenopus DNA replication system in which free plasmid can be replicated synchronously and very efficiently has been reported previously (26).
[ "26" ]
155
8,574
1
false
A nuclear-free Xenopus DNA replication system in which free plasmid can be replicated synchronously and very efficiently has been reported previously.
[ "26" ]
A nuclear-free Xenopus DNA replication system in which free plasmid can be replicated synchronously and very efficiently has been reported previously.
true
true
true
true
true
1,378
3
DISCUSSION
1
26
[ "b26" ]
16,870,720
pmid-9660936
Our preliminary data for quantification of PCNA bound to plasmid beads suggest that the pre-RC may be activated and the replication fork may eventually be formed on most of the immobilized plasmids under the conditions described here.
[ "26" ]
234
8,575
0
false
Our preliminary data for quantification of PCNA bound to plasmid beads suggest that the pre-RC may be activated and the replication fork may eventually be formed on most of the immobilized plasmids under the conditions described here.
[]
Our preliminary data for quantification of PCNA bound to plasmid beads suggest that the pre-RC may be activated and the replication fork may eventually be formed on most of the immobilized plasmids under the conditions described here.
true
true
true
true
true
1,378
3
DISCUSSION
1
26
[ "b26" ]
16,870,720
pmid-9660936
However, in contrast with the nuclear-free system, pre-RC activation on plasmid beads in LSS occurs asynchronously over a long period of time.
[ "26" ]
142
8,576
0
false
However, in contrast with the nuclear-free system, pre-RC activation on plasmid beads in LSS occurs asynchronously over a long period of time.
[]
However, in contrast with the nuclear-free system, pre-RC activation on plasmid beads in LSS occurs asynchronously over a long period of time.
true
true
true
true
true
1,378
3
DISCUSSION
1
26
[ "b26" ]
16,870,720
pmid-9660936
This is probably because functional nuclear structure may not be formed efficiently on the plasmid beads.
[ "26" ]
105
8,577
0
false
This is probably because functional nuclear structure may not be formed efficiently on the plasmid beads.
[]
This is probably because functional nuclear structure may not be formed efficiently on the plasmid beads.
true
true
true
true
true
1,378
3
DISCUSSION
1
26
[ "b26" ]
16,870,720
pmid-9660936
Alternatively, pre-RC activating proteins such as cyclin E may not accumulate efficiently within the nucleus in the presence of the paramagnetic beads.
[ "26" ]
151
8,578
0
false
Alternatively, pre-RC activating proteins such as cyclin E may not accumulate efficiently within the nucleus in the presence of the paramagnetic beads.
[]
Alternatively, pre-RC activating proteins such as cyclin E may not accumulate efficiently within the nucleus in the presence of the paramagnetic beads.
true
true
true
true
true
1,378
4
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-16497662
Despite relatively low DNA synthesis activity, the plasmid replication system described here should still be useful for studying eukaryotic DNA replication and particularly for analyzing replication proteins bound to a plasmid template.
[ "36" ]
236
8,579
0
false
Despite relatively low DNA synthesis activity, the plasmid replication system described here should still be useful for studying eukaryotic DNA replication and particularly for analyzing replication proteins bound to a plasmid template.
[]
Despite relatively low DNA synthesis activity, the plasmid replication system described here should still be useful for studying eukaryotic DNA replication and particularly for analyzing replication proteins bound to a plasmid template.
true
true
true
true
true
1,379
4
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-16497662
The use of magnetic beads allows rapid and simple isolation, and analysis of protein–DNA complexes on the plasmid-coupled beads.
[ "36" ]
128
8,580
0
false
The use of magnetic beads allows rapid and simple isolation, and analysis of protein–DNA complexes on the plasmid-coupled beads.
[]
The use of magnetic beads allows rapid and simple isolation, and analysis of protein–DNA complexes on the plasmid-coupled beads.
true
true
true
true
true
1,379
4
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-16497662
Moreover, because of the rapid timeframe, isolation of relatively unstable complexes may also be possible using this system and, in fact, we have successfully isolated such complexes using circular plasmid beads (36).
[ "36" ]
217
8,581
1
false
Moreover, because of the rapid timeframe, isolation of relatively unstable complexes may also be possible using this system and, in fact, we have successfully isolated such complexes using circular plasmid beads.
[ "36" ]
Moreover, because of the rapid timeframe, isolation of relatively unstable complexes may also be possible using this system and, in fact, we have successfully isolated such complexes using circular plasmid beads.
true
true
true
true
true
1,379
4
DISCUSSION
1
36
[ "b36" ]
16,870,720
pmid-16497662
We believe that the circular plasmid-coupled beads can also be applied for studies using extracts prepared from mammalian cells.
[ "36" ]
128
8,582
0
false
We believe that the circular plasmid-coupled beads can also be applied for studies using extracts prepared from mammalian cells.
[]
We believe that the circular plasmid-coupled beads can also be applied for studies using extracts prepared from mammalian cells.
true
true
true
true
true
1,379
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
Another feature of the replication system is that the supercoiled state in circular DNA is changeable during incubation, despite the attachment of the plasmid to the beads.
[ "45" ]
172
8,583
0
false
Another feature of the replication system is that the supercoiled state in circular DNA is changeable during incubation, despite the attachment of the plasmid to the beads.
[]
Another feature of the replication system is that the supercoiled state in circular DNA is changeable during incubation, despite the attachment of the plasmid to the beads.
true
true
true
true
true
1,380
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
It is known that DNA topology can affect many activities occurring on DNA.
[ "45" ]
74
8,584
0
false
It is known that DNA topology can affect many activities occurring on DNA.
[]
It is known that DNA topology can affect many activities occurring on DNA.
true
true
true
true
true
1,380
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
In fact, our data indicate that MCM proteins can be loaded on to circular DNA more efficiently than onto linear DNA, although ORC and CDC6 seem to bind to both forms of DNA about equally.
[ "45" ]
187
8,585
0
false
In fact, our data indicate that MCM proteins can be loaded on to circular DNA more efficiently than onto linear DNA, although ORC and CDC6 seem to bind to both forms of DNA about equally.
[]
In fact, our data indicate that MCM proteins can be loaded on to circular DNA more efficiently than onto linear DNA, although ORC and CDC6 seem to bind to both forms of DNA about equally.
true
true
true
true
true
1,380
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
This latter observation is somewhat different from the previous finding that purified Drosophila ORC binds preferentially to supercoiled DNA (45).
[ "45" ]
146
8,586
1
false
This latter observation is somewhat different from the previous finding that purified Drosophila ORC binds preferentially to supercoiled DNA.
[ "45" ]
This latter observation is somewhat different from the previous finding that purified Drosophila ORC binds preferentially to supercoiled DNA.
true
true
true
true
true
1,380
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
This discrepancy may be due to different conditions for ORC binding in these experiments; i.e.
[ "45" ]
94
8,587
0
false
This discrepancy may be due to different conditions for ORC binding in these experiments; i.e.
[]
This discrepancy may be due to different conditions for ORC binding in these experiments; i.e.
true
true
true
true
true
1,380
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
a large excess of ORC was used in egg extracts under our conditions.
[ "45" ]
68
8,588
0
false
a large excess of ORC was used in egg extracts under our conditions.
[]
a large excess of ORC was used in egg extracts under our conditions.
false
true
true
true
false
1,380
5
DISCUSSION
1
45
[ "b45" ]
16,870,720
pmid-14765124
Nevertheless, our data suggest that DNA topology may also affect the MCM loading step, in addition to DNA binding of ORC.
[ "45" ]
121
8,589
0
false
Nevertheless, our data suggest that DNA topology may also affect the MCM loading step, in addition to DNA binding of ORC.
[]
Nevertheless, our data suggest that DNA topology may also affect the MCM loading step, in addition to DNA binding of ORC.
true
true
true
true
true
1,380
6
DISCUSSION
1
46
[ "b46" ]
16,870,720
pmid-11172708
In addition to pre-RC formation, nucleosomes are assembled on plasmid beads in egg extracts (A. Zembutsu and S.Waga, unpublished data) and there may be competition among nucleosome assembly, ORC binding and MCM loading on to DNA.
[ "46" ]
229
8,590
0
false
In addition to pre-RC formation, nucleosomes are assembled on plasmid beads in egg extracts (A. Zembutsu and S.Waga, unpublished data) and there may be competition among nucleosome assembly, ORC binding and MCM loading on to DNA.
[]
In addition to pre-RC formation, nucleosomes are assembled on plasmid beads in egg extracts (A. Zembutsu and S.Waga, unpublished data) and there may be competition among nucleosome assembly, ORC binding and MCM loading on to DNA.
true
true
true
true
true
1,381
6
DISCUSSION
1
46
[ "b46" ]
16,870,720
pmid-11172708
Thus, it will be interesting to see whether the nucleosome itself plays any role in the formation of the pre-RC as suggested previously (46).
[ "46" ]
141
8,591
1
false
Thus, it will be interesting to see whether the nucleosome itself plays any role in the formation of the pre-RC as suggested previously.
[ "46" ]
Thus, it will be interesting to see whether the nucleosome itself plays any role in the formation of the pre-RC as suggested previously.
true
true
true
true
true
1,381
6
DISCUSSION
1
46
[ "b46" ]
16,870,720
pmid-11172708
This specific question can be answered using the circular-plasmid replication system presented here.
[ "46" ]
100
8,592
0
false
This specific question can be answered using the circular-plasmid replication system presented here.
[]
This specific question can be answered using the circular-plasmid replication system presented here.
true
true
true
true
true
1,381
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
An understanding of the rate and specificity of assembly of biomolecular complexes is essential for a full appreciation of the mechanisms of biological events.
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
159
8,593
0
false
An understanding of the rate and specificity of assembly of biomolecular complexes is essential for a full appreciation of the mechanisms of biological events.
[]
An understanding of the rate and specificity of assembly of biomolecular complexes is essential for a full appreciation of the mechanisms of biological events.
true
true
true
true
true
1,382
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
Further, currently available information on genome sequences of various organisms can be exploited as a resource for characterizing novel functions of proteins or hypothetical proteins.
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
185
8,594
0
false
Further, currently available information on genome sequences of various organisms can be exploited as a resource for characterizing novel functions of proteins or hypothetical proteins.
[]
Further, currently available information on genome sequences of various organisms can be exploited as a resource for characterizing novel functions of proteins or hypothetical proteins.
true
true
true
true
true
1,382
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
For this purpose, a high-throughput method is required for functional protein analysis.
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
87
8,595
0
false
For this purpose, a high-throughput method is required for functional protein analysis.
[]
For this purpose, a high-throughput method is required for functional protein analysis.
true
true
true
true
true
1,382
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
Fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) have recently been applied to such important biological problems (1–11).
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
171
8,596
0
false
Fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) have recently been applied to such important biological problems.
[ "1–11" ]
Fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) have recently been applied to such important biological problems.
true
true
true
true
true
1,382
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
FCS allows monitoring of the individual movements of fluorescence-labeled molecules through a very tiny area (1,2).
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
115
8,597
0
false
FCS allows monitoring of the individual movements of fluorescence-labeled molecules through a very tiny area.
[ "1,2" ]
FCS allows monitoring of the individual movements of fluorescence-labeled molecules through a very tiny area.
true
true
true
true
true
1,382
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
The time-dependent fluorescence autocorrelation function allows us to analyze the relative proportions of species involved in the diffusion.
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
140
8,598
0
false
The time-dependent fluorescence autocorrelation function allows us to analyze the relative proportions of species involved in the diffusion.
[]
The time-dependent fluorescence autocorrelation function allows us to analyze the relative proportions of species involved in the diffusion.
true
true
true
true
true
1,382
0
INTRODUCTION
1
1
[ "b1", "b11", "b1", "b2", "b3", "b4", "b8", "b5", "b5", "b9", "b6", "b10", "b11" ]
16,914,444
pmid-4818131|pmid-11875038|pmid-4818131|pmid-7517036|pmid-10404975|pmid-12813031|pmid-9657874|pmid-9083691|pmid-9083691|pmid-9772751|pmid-9465029|pmid-9990031|pmid-11875038|pmid-11875038|pmid-8948639|pmid-11168395|pmid-8948639|pmid-3196313
Changes of the proportions can be used to calculate the binding kinetics (3,4,8).
[ "1", "11", "1", "2", "3", "4", "8", "5", "5", "9", "6", "10", "11" ]
81
8,599
0
false
Changes of the proportions can be used to calculate the binding kinetics.
[ "3,4,8" ]
Changes of the proportions can be used to calculate the binding kinetics.
true
true
true
true
true
1,382