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PMC1555579_F5_6770.jpg | What key item or scene is captured in this photo? | Intracellular localization of TBLR1. 3T3 cells respond to apoptotic stimuli with increased expression of TBLR1 and with translocation of TBLR1 into the nucleus. grown in on collagen coated cover slips were used. Untreated cells in the log phase of growth are shown in panels a and d. Apoptosis was induced by either serum starvation (incubation with DMEM containing 0.2% FBS for 16 h) [panels b and e] or growth with100 ng/ml of the topoisomerase inhibitor, camptothecin (CPT) [panels d and f]. The top panels [a, b, and c] were stained for total TBLR1 with anti TBLR1 [117-125] while the bottom panels [d, e, and f] were stained with anti TBLR1β. The images were obtained using a Leitz Orthlux microscope at an original magnification of 600 × with a Nikon 60X phase fluorescence objective (NA 1.4) and captured with a Nikon digital camera. |
PMC1555579_F5_6769.jpg | What does this image primarily show? | Intracellular localization of TBLR1. 3T3 cells respond to apoptotic stimuli with increased expression of TBLR1 and with translocation of TBLR1 into the nucleus. grown in on collagen coated cover slips were used. Untreated cells in the log phase of growth are shown in panels a and d. Apoptosis was induced by either serum starvation (incubation with DMEM containing 0.2% FBS for 16 h) [panels b and e] or growth with100 ng/ml of the topoisomerase inhibitor, camptothecin (CPT) [panels d and f]. The top panels [a, b, and c] were stained for total TBLR1 with anti TBLR1 [117-125] while the bottom panels [d, e, and f] were stained with anti TBLR1β. The images were obtained using a Leitz Orthlux microscope at an original magnification of 600 × with a Nikon 60X phase fluorescence objective (NA 1.4) and captured with a Nikon digital camera. |
PMC1555579_F5_6771.jpg | What is shown in this image? | Intracellular localization of TBLR1. 3T3 cells respond to apoptotic stimuli with increased expression of TBLR1 and with translocation of TBLR1 into the nucleus. grown in on collagen coated cover slips were used. Untreated cells in the log phase of growth are shown in panels a and d. Apoptosis was induced by either serum starvation (incubation with DMEM containing 0.2% FBS for 16 h) [panels b and e] or growth with100 ng/ml of the topoisomerase inhibitor, camptothecin (CPT) [panels d and f]. The top panels [a, b, and c] were stained for total TBLR1 with anti TBLR1 [117-125] while the bottom panels [d, e, and f] were stained with anti TBLR1β. The images were obtained using a Leitz Orthlux microscope at an original magnification of 600 × with a Nikon 60X phase fluorescence objective (NA 1.4) and captured with a Nikon digital camera. |
PMC1555579_F5_6772.jpg | What is the central feature of this picture? | Intracellular localization of TBLR1. 3T3 cells respond to apoptotic stimuli with increased expression of TBLR1 and with translocation of TBLR1 into the nucleus. grown in on collagen coated cover slips were used. Untreated cells in the log phase of growth are shown in panels a and d. Apoptosis was induced by either serum starvation (incubation with DMEM containing 0.2% FBS for 16 h) [panels b and e] or growth with100 ng/ml of the topoisomerase inhibitor, camptothecin (CPT) [panels d and f]. The top panels [a, b, and c] were stained for total TBLR1 with anti TBLR1 [117-125] while the bottom panels [d, e, and f] were stained with anti TBLR1β. The images were obtained using a Leitz Orthlux microscope at an original magnification of 600 × with a Nikon 60X phase fluorescence objective (NA 1.4) and captured with a Nikon digital camera. |
PMC1555595_F2_6788.jpg | What is the core subject represented in this visual? | Syntaxin 1 is associated with conventional synapses in the inner plexiform layer (IPL). A–C: Confocal microscopy of double labeling for syntaxin 1 and synapsin I, a marker for conventional synapses made by amacrine cells. There is appreciable colocalization of labeling in conventional synaptic terminals (arrows). However, syntaxin 1 labeling is not restricted the presynaptic terminal and is often seen independent of synapsin I labeling. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 1 and synapsin I double labeling from the area indicated by the box in panels A–C confirms the localization of syntaxin 1 to conventional synapses. GCL, ganglion cell layer. Images shown from a projection of 12 optical sections with total thickness of 1.6 μm. Scale bars = 10 μm for A–C; 5 μm for D–F. |
PMC1555595_F3_6777.jpg | What stands out most in this visual? | Syntaxin 3 does not colocalize with syntaxin 1. A–C: Syntaxin 3 is localized to the glutamatergic photoreceptor terminals in the outer plexiform layer (OPL) and numerous bipolar cell terminals in the inner plexiform layer (IPL). Labeling is also present in the cell bodies and inner segments of the photoreceptors and in bipolar cell bodies and axons. Syntaxin 1 labeling is restricted to amacrine cells and amacrine cell processes. Images shown from a projection of 10 optical sections with total thickness of 1.93 μm. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and syntaxin 1 immunolabeling at higher magnification confirms that these syntaxin isoforms are present in different sets of synaptic terminals. Arrows indicate bipolar cell terminals labeled for syntaxin 3. Images shown from a projection of 15 optical sections with total thickness of 2.04 μm. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F3_6775.jpg | What is the dominant medical problem in this image? | Syntaxin 3 does not colocalize with syntaxin 1. A–C: Syntaxin 3 is localized to the glutamatergic photoreceptor terminals in the outer plexiform layer (OPL) and numerous bipolar cell terminals in the inner plexiform layer (IPL). Labeling is also present in the cell bodies and inner segments of the photoreceptors and in bipolar cell bodies and axons. Syntaxin 1 labeling is restricted to amacrine cells and amacrine cell processes. Images shown from a projection of 10 optical sections with total thickness of 1.93 μm. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and syntaxin 1 immunolabeling at higher magnification confirms that these syntaxin isoforms are present in different sets of synaptic terminals. Arrows indicate bipolar cell terminals labeled for syntaxin 3. Images shown from a projection of 15 optical sections with total thickness of 2.04 μm. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F3_6776.jpg | What is the principal component of this image? | Syntaxin 3 does not colocalize with syntaxin 1. A–C: Syntaxin 3 is localized to the glutamatergic photoreceptor terminals in the outer plexiform layer (OPL) and numerous bipolar cell terminals in the inner plexiform layer (IPL). Labeling is also present in the cell bodies and inner segments of the photoreceptors and in bipolar cell bodies and axons. Syntaxin 1 labeling is restricted to amacrine cells and amacrine cell processes. Images shown from a projection of 10 optical sections with total thickness of 1.93 μm. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and syntaxin 1 immunolabeling at higher magnification confirms that these syntaxin isoforms are present in different sets of synaptic terminals. Arrows indicate bipolar cell terminals labeled for syntaxin 3. Images shown from a projection of 15 optical sections with total thickness of 2.04 μm. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F4_6784.jpg | What is the core subject represented in this visual? | Syntaxin 3 is associated with the glutamatergic ribbon synapses of photoreceptors and bipolar cells. A–C: Labeling for syntaxin 3 and VGLUT1, a marker for ribbon synapses, colocalize extensively in the photoreceptor terminals in the OPL and in bipolar cell terminals in the IPL. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and VGLUT1 immunolabeling in the IPL at higher magnification confirms that all bipolar cell terminals contain syntaxin 3. Images shown are from a single optical section. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F4_6782.jpg | What is the core subject represented in this visual? | Syntaxin 3 is associated with the glutamatergic ribbon synapses of photoreceptors and bipolar cells. A–C: Labeling for syntaxin 3 and VGLUT1, a marker for ribbon synapses, colocalize extensively in the photoreceptor terminals in the OPL and in bipolar cell terminals in the IPL. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and VGLUT1 immunolabeling in the IPL at higher magnification confirms that all bipolar cell terminals contain syntaxin 3. Images shown are from a single optical section. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F4_6785.jpg | What key item or scene is captured in this photo? | Syntaxin 3 is associated with the glutamatergic ribbon synapses of photoreceptors and bipolar cells. A–C: Labeling for syntaxin 3 and VGLUT1, a marker for ribbon synapses, colocalize extensively in the photoreceptor terminals in the OPL and in bipolar cell terminals in the IPL. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and VGLUT1 immunolabeling in the IPL at higher magnification confirms that all bipolar cell terminals contain syntaxin 3. Images shown are from a single optical section. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F4_6783.jpg | What is the core subject represented in this visual? | Syntaxin 3 is associated with the glutamatergic ribbon synapses of photoreceptors and bipolar cells. A–C: Labeling for syntaxin 3 and VGLUT1, a marker for ribbon synapses, colocalize extensively in the photoreceptor terminals in the OPL and in bipolar cell terminals in the IPL. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and VGLUT1 immunolabeling in the IPL at higher magnification confirms that all bipolar cell terminals contain syntaxin 3. Images shown are from a single optical section. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F4_6780.jpg | What is shown in this image? | Syntaxin 3 is associated with the glutamatergic ribbon synapses of photoreceptors and bipolar cells. A–C: Labeling for syntaxin 3 and VGLUT1, a marker for ribbon synapses, colocalize extensively in the photoreceptor terminals in the OPL and in bipolar cell terminals in the IPL. Box indicates area shown in panels D–F. D–F: Examination of syntaxin 3 and VGLUT1 immunolabeling in the IPL at higher magnification confirms that all bipolar cell terminals contain syntaxin 3. Images shown are from a single optical section. IS, inner segments; ONL, Outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–F. |
PMC1555595_F7_6795.jpg | What stands out most in this visual? | Syntaxin 2 shows little colocalization with syntaxin I. A–C: Syntaxin 2 labeling is present in numerous amacrine cells (A) and small puncta throughout the inner plexiform layer (IPL; arrows and arrowheads). Syntaxin 1 is widely expressed in amacrine cell bodies, processes and presynaptic terminals. At low magnification, there is some apparent colocalization of these two syntaxin isoforms in conventional terminals (arrowheads), although many syntaxin 2-positive terminals do not show labeling for syntaxin 1 (arrowheads). Similarly, Most labeling for syntaxin 1 does not colocalize with labeling for syntaxin 2. Images shown from a projection of 13 optical sections with total thickness of 1.75 μm. D–F: Inspection at higher magnification shows that syntaxin 2 labeling is typically present in puncta that show little or no syntaxin 1 labeling, although a few puncta that show strong colocalization of labels are present (arrowheads). Colocalization associated with diffuse labeling, which is probably not associated with release sites, is also present. Images shown from a single optical plane. INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars = 10 μm for A–C; 5 μm for D–F. |
PMC1555595_F14_6803.jpg | What is being portrayed in this visual content? | Syntaxin 4-positive puncta in the distal inner plexiform layer (IPL) do not arise from dopaminergic or GABAergic CD15-positive amacrine cells. A–C: Syntaxin 4-positive puncta form a plexus along the border of the inner nuclear layer (INL) and IPL that co-stratifies with the processes of dopaminergic amacrine cells (A) labeled for tyrosine hydroxylase (TH). Syntaxin 4 and TH labeling do not colocalize (see D–F), but syntaxin 4-positive puncta contact the processes and cell bodies of the dopaminergic cells (arrowheads). Images shown from a projection of 73 optical sections with total thickness of 10.5 μm. D–F: Higher magnification image of syntaxin 4-positive puncta (arrows and arrowheads) and TH-positive dopaminergic amacrine cell processes in the IPL in a single optical plane. There is no colocalization of labels indicating that the syntaxin 4-positive puncta do not arise from the dopaminergic amacrine cells, but there is often contact between the syntaxin 4-positive puncta and the TH-positive processes (arrowheads). G–I: Syntaxin 4-positive puncta (arrows) form a distinctive plexus that co-stratifies with processes from GABAergic CD15-positive amacrine cells (A). The processes of the CD15-positive amacrine cells do not label for syntaxin 4, but there is contact between syntaxin 4-positive puncta and the processes and cell body of CD15-positive amacrine cells (arrowhead). Images shown from a projection of 57 optical sections with total thickness of 8.16 μm. J–L: Higher magnification image of syntaxin 4 and CD15 double labeling visualized in a single optical plane. Syntaxin 4-positive puncta (arrows) co-stratify with the CD15-positive plexus in the IPL and often contact CD15-positive amacrine cells at the cell body or on processes (arrowhead). Images shown from a projection of 14 optical sections with total thickness of 1.89 μm. GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–I; 5 μm for J–L. |
PMC1555595_F14_6799.jpg | What is the principal component of this image? | Syntaxin 4-positive puncta in the distal inner plexiform layer (IPL) do not arise from dopaminergic or GABAergic CD15-positive amacrine cells. A–C: Syntaxin 4-positive puncta form a plexus along the border of the inner nuclear layer (INL) and IPL that co-stratifies with the processes of dopaminergic amacrine cells (A) labeled for tyrosine hydroxylase (TH). Syntaxin 4 and TH labeling do not colocalize (see D–F), but syntaxin 4-positive puncta contact the processes and cell bodies of the dopaminergic cells (arrowheads). Images shown from a projection of 73 optical sections with total thickness of 10.5 μm. D–F: Higher magnification image of syntaxin 4-positive puncta (arrows and arrowheads) and TH-positive dopaminergic amacrine cell processes in the IPL in a single optical plane. There is no colocalization of labels indicating that the syntaxin 4-positive puncta do not arise from the dopaminergic amacrine cells, but there is often contact between the syntaxin 4-positive puncta and the TH-positive processes (arrowheads). G–I: Syntaxin 4-positive puncta (arrows) form a distinctive plexus that co-stratifies with processes from GABAergic CD15-positive amacrine cells (A). The processes of the CD15-positive amacrine cells do not label for syntaxin 4, but there is contact between syntaxin 4-positive puncta and the processes and cell body of CD15-positive amacrine cells (arrowhead). Images shown from a projection of 57 optical sections with total thickness of 8.16 μm. J–L: Higher magnification image of syntaxin 4 and CD15 double labeling visualized in a single optical plane. Syntaxin 4-positive puncta (arrows) co-stratify with the CD15-positive plexus in the IPL and often contact CD15-positive amacrine cells at the cell body or on processes (arrowhead). Images shown from a projection of 14 optical sections with total thickness of 1.89 μm. GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–I; 5 μm for J–L. |
PMC1555595_F14_6800.jpg | Can you identify the primary element in this image? | Syntaxin 4-positive puncta in the distal inner plexiform layer (IPL) do not arise from dopaminergic or GABAergic CD15-positive amacrine cells. A–C: Syntaxin 4-positive puncta form a plexus along the border of the inner nuclear layer (INL) and IPL that co-stratifies with the processes of dopaminergic amacrine cells (A) labeled for tyrosine hydroxylase (TH). Syntaxin 4 and TH labeling do not colocalize (see D–F), but syntaxin 4-positive puncta contact the processes and cell bodies of the dopaminergic cells (arrowheads). Images shown from a projection of 73 optical sections with total thickness of 10.5 μm. D–F: Higher magnification image of syntaxin 4-positive puncta (arrows and arrowheads) and TH-positive dopaminergic amacrine cell processes in the IPL in a single optical plane. There is no colocalization of labels indicating that the syntaxin 4-positive puncta do not arise from the dopaminergic amacrine cells, but there is often contact between the syntaxin 4-positive puncta and the TH-positive processes (arrowheads). G–I: Syntaxin 4-positive puncta (arrows) form a distinctive plexus that co-stratifies with processes from GABAergic CD15-positive amacrine cells (A). The processes of the CD15-positive amacrine cells do not label for syntaxin 4, but there is contact between syntaxin 4-positive puncta and the processes and cell body of CD15-positive amacrine cells (arrowhead). Images shown from a projection of 57 optical sections with total thickness of 8.16 μm. J–L: Higher magnification image of syntaxin 4 and CD15 double labeling visualized in a single optical plane. Syntaxin 4-positive puncta (arrows) co-stratify with the CD15-positive plexus in the IPL and often contact CD15-positive amacrine cells at the cell body or on processes (arrowhead). Images shown from a projection of 14 optical sections with total thickness of 1.89 μm. GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–I; 5 μm for J–L. |
PMC1555595_F14_6807.jpg | What's the most prominent thing you notice in this picture? | Syntaxin 4-positive puncta in the distal inner plexiform layer (IPL) do not arise from dopaminergic or GABAergic CD15-positive amacrine cells. A–C: Syntaxin 4-positive puncta form a plexus along the border of the inner nuclear layer (INL) and IPL that co-stratifies with the processes of dopaminergic amacrine cells (A) labeled for tyrosine hydroxylase (TH). Syntaxin 4 and TH labeling do not colocalize (see D–F), but syntaxin 4-positive puncta contact the processes and cell bodies of the dopaminergic cells (arrowheads). Images shown from a projection of 73 optical sections with total thickness of 10.5 μm. D–F: Higher magnification image of syntaxin 4-positive puncta (arrows and arrowheads) and TH-positive dopaminergic amacrine cell processes in the IPL in a single optical plane. There is no colocalization of labels indicating that the syntaxin 4-positive puncta do not arise from the dopaminergic amacrine cells, but there is often contact between the syntaxin 4-positive puncta and the TH-positive processes (arrowheads). G–I: Syntaxin 4-positive puncta (arrows) form a distinctive plexus that co-stratifies with processes from GABAergic CD15-positive amacrine cells (A). The processes of the CD15-positive amacrine cells do not label for syntaxin 4, but there is contact between syntaxin 4-positive puncta and the processes and cell body of CD15-positive amacrine cells (arrowhead). Images shown from a projection of 57 optical sections with total thickness of 8.16 μm. J–L: Higher magnification image of syntaxin 4 and CD15 double labeling visualized in a single optical plane. Syntaxin 4-positive puncta (arrows) co-stratify with the CD15-positive plexus in the IPL and often contact CD15-positive amacrine cells at the cell body or on processes (arrowhead). Images shown from a projection of 14 optical sections with total thickness of 1.89 μm. GCL, ganglion cell layer. Scale bars = 20 μm for A–C; 10 μm for D–I; 5 μm for J–L. |
PMC1555629_F3_6810.jpg | What is the principal component of this image? | Transatrial lead implantation in a 12 year old boy with complex cardiac malformations. He had several open heart procedures and finally in 1995 a modified Fontan procedure with the addition of a fenestrated atrial baffle was accomplished. Sick sinus syndrome with bradycardia and dizziness was diagnosed in 2001. Because of the caval pulmonary connection a venous approach for lead insertion from cranial was impossible. Therefore, after median sternotomy a bipolar screw-in lead was implanted transatrially into the venous system (1) together with a right ventricular epicardial bipolar, steroid-eluting epicardial electrode (2). The pacemaker was programmed to DDDR mode, with the AV-conduction time so long that intermittent atrial pacemacer stimulation allowed physiologic stimulation of the ventricle. As an atrial back up lead a second epicardial bipolar steroid-eluting electrode was placed on the right atrium (3) with the lead body tunnelled to the generator pocket. In case of transatrial lead failure (e.g. exit block), revision would not neccessitate a rethoracotomy, but only an incision into the abdominal generator pocket and the exchange of the epicardial atrial lead. |
PMC1557485_F1_6811.jpg | What is shown in this image? | CT Angiogram obtained prior to infusion of rhAPC demonstrating no evidence of pulmonary embolism. |
PMC1557485_F2_6812.jpg | What is the core subject represented in this visual? | CT Angiogram obtained after 78 hours of infusion of rhAPC demonstrating a large saddle pulmonary embolism. |
PMC1557485_F2_6814.jpg | What is the dominant medical problem in this image? | CT Angiogram obtained after 78 hours of infusion of rhAPC demonstrating a large saddle pulmonary embolism. |
PMC1557497_F1_6815.jpg | What does this image primarily show? | Angiographic appearance of aortic CoA (white arrow) and ascending aortic aneurysm (white line arrow) in case 1. |
PMC1557507_F1_6816.jpg | What object or scene is depicted here? | Lymphoscintigraphy demonstrating the location of the primary tumor in the upper inner quadrant of the right breast (large central area of radioactivity) as well as the two sentinel lymph nodes. |
PMC1557511_F1_6821.jpg | What is the principal component of this image? | ChAT in adult primate ovaries. A: ChAT is detectable using immunohistochemical methods in a monkey ovary only in the GC layer (arrows) of several large antral follicles. Note that the areas between the sectioned follicles are devoid of any immunoreactive structures. Bar = 500 μm. B: A consecutive section of the one shown in A is depicted and used as a control (incubation with non-immune mouse normal serum instead of the specific antibody). The asterisk denotes a large antral follicle. Bar = 200 μm. C. Immunoreactive GCs of a large antral follicle in a human ovary. Bar = 50 μm. |
PMC1557511_F1_6822.jpg | What object or scene is depicted here? | ChAT in adult primate ovaries. A: ChAT is detectable using immunohistochemical methods in a monkey ovary only in the GC layer (arrows) of several large antral follicles. Note that the areas between the sectioned follicles are devoid of any immunoreactive structures. Bar = 500 μm. B: A consecutive section of the one shown in A is depicted and used as a control (incubation with non-immune mouse normal serum instead of the specific antibody). The asterisk denotes a large antral follicle. Bar = 200 μm. C. Immunoreactive GCs of a large antral follicle in a human ovary. Bar = 50 μm. |
PMC1557511_F1_6820.jpg | What is the dominant medical problem in this image? | ChAT in adult primate ovaries. A: ChAT is detectable using immunohistochemical methods in a monkey ovary only in the GC layer (arrows) of several large antral follicles. Note that the areas between the sectioned follicles are devoid of any immunoreactive structures. Bar = 500 μm. B: A consecutive section of the one shown in A is depicted and used as a control (incubation with non-immune mouse normal serum instead of the specific antibody). The asterisk denotes a large antral follicle. Bar = 200 μm. C. Immunoreactive GCs of a large antral follicle in a human ovary. Bar = 50 μm. |
PMC1557511_F3_6817.jpg | What is the focal point of this photograph? | Absence of ChAT in embryonic mouse ovary and morphology of ChAT (-/-) mouse ovary. A: ChAT is not detectable using immunohistochemical methods in a mouse ovary at day 18 p.c. Bar = 50 μm. B-C: H.E. stained sections of the ovary of an embryonic (day 18 p.c.) age-matched wild-type ovary (+/+) and a mutant mouse null for ChAT (-/-). Bars = 60 μm. |
PMC1557513_F3_6824.jpg | What's the most prominent thing you notice in this picture? | Identification of mutants defective in intracellular growth in HepG2 cells. Individual transposon-insertion mutants were incubated with HepG2 cells for two hours, treated with gentamicin, incubated for an additional 24 hrs, then the HepG2 cells were lysed. The lysates were transferred to MHA plates with a 48-pin replicator and incubated for 48 hours. Representative mutants that showed no or reduced growth after 48 hrs are indicated by arrowheads. |
PMC1557518_F3_6830.jpg | What can you see in this picture? | Clinical examples of cone beam (right side) compared to diagnostic treatment planning CT (left side). |
PMC1557518_F3_6827.jpg | What is shown in this image? | Clinical examples of cone beam (right side) compared to diagnostic treatment planning CT (left side). |
PMC1557518_F3_6825.jpg | What is the central feature of this picture? | Clinical examples of cone beam (right side) compared to diagnostic treatment planning CT (left side). |
PMC1557518_F3_6828.jpg | What key item or scene is captured in this photo? | Clinical examples of cone beam (right side) compared to diagnostic treatment planning CT (left side). |
PMC1557518_F3_6831.jpg | What is being portrayed in this visual content? | Clinical examples of cone beam (right side) compared to diagnostic treatment planning CT (left side). |
PMC1557518_F3_6829.jpg | What is the dominant medical problem in this image? | Clinical examples of cone beam (right side) compared to diagnostic treatment planning CT (left side). |
PMC1557539_F1_6832.jpg | What is the principal component of this image? | Trans-thoracic echocardiography (2-D apical, four cavities view) showing a giant left atrium (73 cm2). Note the marked deviation of the atrial septum (white arrow). LA: left atrium, LV: left ventricle. |
PMC1557584_ppat-0020086-g002_6847.jpg | What is the core subject represented in this visual? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6846.jpg | What is being portrayed in this visual content? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6854.jpg | Describe the main subject of this image. | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6853.jpg | What's the most prominent thing you notice in this picture? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6845.jpg | What is shown in this image? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6855.jpg | What stands out most in this visual? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6849.jpg | What is the dominant medical problem in this image? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6848.jpg | What is the principal component of this image? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6851.jpg | What can you see in this picture? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6856.jpg | What is shown in this image? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g002_6850.jpg | What does this image primarily show? | MLN Histopathology during WT or pIB1− InfectionBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−, and MLN were processed for H&E staining 4 d later. MLN sections from uninfected (Uninf.) (A–D), WT infected (E–H), and pIB1− infected (I–L) are shown at 15×, 40×, 90×, and 450× magnification. White boxes indicate magnified areas in the next slide. White arrow points to the bacterial foci, black arrow to neutrophils. Scale bars correspond to 133 μm for 15× magnification, 50 μm for 40× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from 16 MLN infected with WT or 14 MLN infected with pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6833.jpg | What can you see in this picture? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6842.jpg | What is the principal component of this image? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6839.jpg | What is being portrayed in this visual content? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6838.jpg | What object or scene is depicted here? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6836.jpg | What can you see in this picture? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6840.jpg | What is being portrayed in this visual content? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6837.jpg | What is the core subject represented in this visual? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6834.jpg | What is the main focus of this visual representation? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6835.jpg | What is the core subject represented in this visual? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g003_6843.jpg | What stands out most in this visual? |
Yptb Localizes in the Cortex and Paracortex of the MLNBALB/c mice were intragastrically inoculated with 2 × 109 WT or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested and stained for Yptb followed by hematoxylin staining. Picture sections of uninfected (A–C), WT infected (D–I), and pIB1− infected (J–L) MLN were taken at 150×, 900×, and 4,500× magnification. White boxes indicate magnified areas in the next slide. Arrows point to Yptb microcolonies. Scale bars correspond to 133 μm for 15× magnification, 22 μm for 90× magnification, and 4.4 μm for 450× magnification. Pictures shown are representative of multiple fields and samples from MLN infected with WT or pIB1−.C, cortex; gc, germinal center; M, medulla; P, paracortex. |
PMC1557584_ppat-0020086-g004_6857.jpg | What can you see in this picture? | pTTSS Mutants Are Adjacent to B and T Lymphocytes Whereas WT Is Adjacent to B and T Lymphocytes and CD11b+ CellsBALB/c mice were intragastrically inoculated with 2 × 109 WT IP2666 or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested, sectioned, and examined by fluorescence microscopy. Staining with antibodies to Yptb (red) (A), (D), (G), and (J), to CD4-CD8-B220 (green) (B) and (E), or to CD11b (green) (H) and (K) was performed, and images merged (C), (F), (I), and (L). Pictures show the cortex–paracortex and are representative of multiple fields and samples for WT-infected mice and pIB1 mice stained with CD4-CD8-B220. Fewer fields had pIB1 bacteria and CD11b+ cells. Scale bars correspond to 22 μm for 900× magnification. |
PMC1557584_ppat-0020086-g004_6863.jpg | What is the principal component of this image? | pTTSS Mutants Are Adjacent to B and T Lymphocytes Whereas WT Is Adjacent to B and T Lymphocytes and CD11b+ CellsBALB/c mice were intragastrically inoculated with 2 × 109 WT IP2666 or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested, sectioned, and examined by fluorescence microscopy. Staining with antibodies to Yptb (red) (A), (D), (G), and (J), to CD4-CD8-B220 (green) (B) and (E), or to CD11b (green) (H) and (K) was performed, and images merged (C), (F), (I), and (L). Pictures show the cortex–paracortex and are representative of multiple fields and samples for WT-infected mice and pIB1 mice stained with CD4-CD8-B220. Fewer fields had pIB1 bacteria and CD11b+ cells. Scale bars correspond to 22 μm for 900× magnification. |
PMC1557584_ppat-0020086-g004_6867.jpg | What is the main focus of this visual representation? | pTTSS Mutants Are Adjacent to B and T Lymphocytes Whereas WT Is Adjacent to B and T Lymphocytes and CD11b+ CellsBALB/c mice were intragastrically inoculated with 2 × 109 WT IP2666 or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested, sectioned, and examined by fluorescence microscopy. Staining with antibodies to Yptb (red) (A), (D), (G), and (J), to CD4-CD8-B220 (green) (B) and (E), or to CD11b (green) (H) and (K) was performed, and images merged (C), (F), (I), and (L). Pictures show the cortex–paracortex and are representative of multiple fields and samples for WT-infected mice and pIB1 mice stained with CD4-CD8-B220. Fewer fields had pIB1 bacteria and CD11b+ cells. Scale bars correspond to 22 μm for 900× magnification. |
PMC1557584_ppat-0020086-g004_6865.jpg | What can you see in this picture? | pTTSS Mutants Are Adjacent to B and T Lymphocytes Whereas WT Is Adjacent to B and T Lymphocytes and CD11b+ CellsBALB/c mice were intragastrically inoculated with 2 × 109 WT IP2666 or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested, sectioned, and examined by fluorescence microscopy. Staining with antibodies to Yptb (red) (A), (D), (G), and (J), to CD4-CD8-B220 (green) (B) and (E), or to CD11b (green) (H) and (K) was performed, and images merged (C), (F), (I), and (L). Pictures show the cortex–paracortex and are representative of multiple fields and samples for WT-infected mice and pIB1 mice stained with CD4-CD8-B220. Fewer fields had pIB1 bacteria and CD11b+ cells. Scale bars correspond to 22 μm for 900× magnification. |
PMC1557584_ppat-0020086-g004_6860.jpg | What does this image primarily show? | pTTSS Mutants Are Adjacent to B and T Lymphocytes Whereas WT Is Adjacent to B and T Lymphocytes and CD11b+ CellsBALB/c mice were intragastrically inoculated with 2 × 109 WT IP2666 or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested, sectioned, and examined by fluorescence microscopy. Staining with antibodies to Yptb (red) (A), (D), (G), and (J), to CD4-CD8-B220 (green) (B) and (E), or to CD11b (green) (H) and (K) was performed, and images merged (C), (F), (I), and (L). Pictures show the cortex–paracortex and are representative of multiple fields and samples for WT-infected mice and pIB1 mice stained with CD4-CD8-B220. Fewer fields had pIB1 bacteria and CD11b+ cells. Scale bars correspond to 22 μm for 900× magnification. |
PMC1557584_ppat-0020086-g004_6864.jpg | What is being portrayed in this visual content? | pTTSS Mutants Are Adjacent to B and T Lymphocytes Whereas WT Is Adjacent to B and T Lymphocytes and CD11b+ CellsBALB/c mice were intragastrically inoculated with 2 × 109 WT IP2666 or IP2666 pIB1−. At 4 d post-inoculation, MLN were harvested, sectioned, and examined by fluorescence microscopy. Staining with antibodies to Yptb (red) (A), (D), (G), and (J), to CD4-CD8-B220 (green) (B) and (E), or to CD11b (green) (H) and (K) was performed, and images merged (C), (F), (I), and (L). Pictures show the cortex–paracortex and are representative of multiple fields and samples for WT-infected mice and pIB1 mice stained with CD4-CD8-B220. Fewer fields had pIB1 bacteria and CD11b+ cells. Scale bars correspond to 22 μm for 900× magnification. |
PMC1557590_F4_6870.jpg | What does this image primarily show? | leiomyosarcoma. (a) Malignant BSCTs cytology, cellular smear with sheets of spindle cells, Papanicolaou staining × 400. (b) Another field showing neoplastic ovoid cells, Papanicolaou staining × 400. (c) Well circumscribed tumor pushing the normal ductal cells at the periphery, H&E × 10. (d) Spindle cells are merging from blood vessels, H&E, × 10. (e) Desmin IHC highlighting cells merging from the blood vessels, DAB Hx × 400. (f) Intersecting fascicles of pleomorphic malignant spindle cells having cigar shaped blunt ended nuclei, H&E, × 400. (g) Desmin IHC, DAB, Hx × 400. See additional files 16, 17, 18, 1920, 21, 22 for higher resolution images. |
PMC1557590_F4_6874.jpg | What is the core subject represented in this visual? | leiomyosarcoma. (a) Malignant BSCTs cytology, cellular smear with sheets of spindle cells, Papanicolaou staining × 400. (b) Another field showing neoplastic ovoid cells, Papanicolaou staining × 400. (c) Well circumscribed tumor pushing the normal ductal cells at the periphery, H&E × 10. (d) Spindle cells are merging from blood vessels, H&E, × 10. (e) Desmin IHC highlighting cells merging from the blood vessels, DAB Hx × 400. (f) Intersecting fascicles of pleomorphic malignant spindle cells having cigar shaped blunt ended nuclei, H&E, × 400. (g) Desmin IHC, DAB, Hx × 400. See additional files 16, 17, 18, 1920, 21, 22 for higher resolution images. |
PMC1557590_F4_6873.jpg | What key item or scene is captured in this photo? | leiomyosarcoma. (a) Malignant BSCTs cytology, cellular smear with sheets of spindle cells, Papanicolaou staining × 400. (b) Another field showing neoplastic ovoid cells, Papanicolaou staining × 400. (c) Well circumscribed tumor pushing the normal ductal cells at the periphery, H&E × 10. (d) Spindle cells are merging from blood vessels, H&E, × 10. (e) Desmin IHC highlighting cells merging from the blood vessels, DAB Hx × 400. (f) Intersecting fascicles of pleomorphic malignant spindle cells having cigar shaped blunt ended nuclei, H&E, × 400. (g) Desmin IHC, DAB, Hx × 400. See additional files 16, 17, 18, 1920, 21, 22 for higher resolution images. |
PMC1557590_F4_6875.jpg | What is the main focus of this visual representation? | leiomyosarcoma. (a) Malignant BSCTs cytology, cellular smear with sheets of spindle cells, Papanicolaou staining × 400. (b) Another field showing neoplastic ovoid cells, Papanicolaou staining × 400. (c) Well circumscribed tumor pushing the normal ductal cells at the periphery, H&E × 10. (d) Spindle cells are merging from blood vessels, H&E, × 10. (e) Desmin IHC highlighting cells merging from the blood vessels, DAB Hx × 400. (f) Intersecting fascicles of pleomorphic malignant spindle cells having cigar shaped blunt ended nuclei, H&E, × 400. (g) Desmin IHC, DAB, Hx × 400. See additional files 16, 17, 18, 1920, 21, 22 for higher resolution images. |
PMC1557590_F4_6871.jpg | What is the main focus of this visual representation? | leiomyosarcoma. (a) Malignant BSCTs cytology, cellular smear with sheets of spindle cells, Papanicolaou staining × 400. (b) Another field showing neoplastic ovoid cells, Papanicolaou staining × 400. (c) Well circumscribed tumor pushing the normal ductal cells at the periphery, H&E × 10. (d) Spindle cells are merging from blood vessels, H&E, × 10. (e) Desmin IHC highlighting cells merging from the blood vessels, DAB Hx × 400. (f) Intersecting fascicles of pleomorphic malignant spindle cells having cigar shaped blunt ended nuclei, H&E, × 400. (g) Desmin IHC, DAB, Hx × 400. See additional files 16, 17, 18, 1920, 21, 22 for higher resolution images. |
PMC1557590_F4_6869.jpg | What object or scene is depicted here? | leiomyosarcoma. (a) Malignant BSCTs cytology, cellular smear with sheets of spindle cells, Papanicolaou staining × 400. (b) Another field showing neoplastic ovoid cells, Papanicolaou staining × 400. (c) Well circumscribed tumor pushing the normal ductal cells at the periphery, H&E × 10. (d) Spindle cells are merging from blood vessels, H&E, × 10. (e) Desmin IHC highlighting cells merging from the blood vessels, DAB Hx × 400. (f) Intersecting fascicles of pleomorphic malignant spindle cells having cigar shaped blunt ended nuclei, H&E, × 400. (g) Desmin IHC, DAB, Hx × 400. See additional files 16, 17, 18, 1920, 21, 22 for higher resolution images. |
PMC1557671_F4_6877.jpg | What is the core subject represented in this visual? | Expression of CD164 in prostate cancer cell lines is responsive to CXCL12. To determine if the prostate cancer cells express CD164 protein, and whether its expression was altered by CXCL12, PC3 and LNCaP C4-2B cells were treated with PBS or 200 ng/ml CXCL12 for 2 h, fixed and stained with anti-CD164 mAb or an IgG isotype matched control, and photographed at 40X. Scale bars = 100 μm. The cancer cells demonstatrate perinuclear and cytoplasmic expression of CD164 in nearly all of the cells under basal conditions, and abundantly express CD164 following CXCL12 stimulation. |
PMC1557671_F4_6884.jpg | What is the principal component of this image? | Expression of CD164 in prostate cancer cell lines is responsive to CXCL12. To determine if the prostate cancer cells express CD164 protein, and whether its expression was altered by CXCL12, PC3 and LNCaP C4-2B cells were treated with PBS or 200 ng/ml CXCL12 for 2 h, fixed and stained with anti-CD164 mAb or an IgG isotype matched control, and photographed at 40X. Scale bars = 100 μm. The cancer cells demonstatrate perinuclear and cytoplasmic expression of CD164 in nearly all of the cells under basal conditions, and abundantly express CD164 following CXCL12 stimulation. |
PMC1557671_F4_6879.jpg | What stands out most in this visual? | Expression of CD164 in prostate cancer cell lines is responsive to CXCL12. To determine if the prostate cancer cells express CD164 protein, and whether its expression was altered by CXCL12, PC3 and LNCaP C4-2B cells were treated with PBS or 200 ng/ml CXCL12 for 2 h, fixed and stained with anti-CD164 mAb or an IgG isotype matched control, and photographed at 40X. Scale bars = 100 μm. The cancer cells demonstatrate perinuclear and cytoplasmic expression of CD164 in nearly all of the cells under basal conditions, and abundantly express CD164 following CXCL12 stimulation. |
PMC1557671_F4_6883.jpg | What's the most prominent thing you notice in this picture? | Expression of CD164 in prostate cancer cell lines is responsive to CXCL12. To determine if the prostate cancer cells express CD164 protein, and whether its expression was altered by CXCL12, PC3 and LNCaP C4-2B cells were treated with PBS or 200 ng/ml CXCL12 for 2 h, fixed and stained with anti-CD164 mAb or an IgG isotype matched control, and photographed at 40X. Scale bars = 100 μm. The cancer cells demonstatrate perinuclear and cytoplasmic expression of CD164 in nearly all of the cells under basal conditions, and abundantly express CD164 following CXCL12 stimulation. |
PMC1557733_F4_6895.jpg | What's the most prominent thing you notice in this picture? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6888.jpg | What is the main focus of this visual representation? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6889.jpg | What is the main focus of this visual representation? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6892.jpg | What can you see in this picture? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6896.jpg | What key item or scene is captured in this photo? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6890.jpg | What is shown in this image? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6885.jpg | What object or scene is depicted here? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6891.jpg | What is the principal component of this image? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6893.jpg | Can you identify the primary element in this image? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6887.jpg | What object or scene is depicted here? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6886.jpg | What is the central feature of this picture? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557733_F4_6894.jpg | What stands out most in this visual? | Keratin 6ab (K6ab)-null mammary gland whole-mount analysis. Mammary glands were isolated from intact wild-type (WT) or K6ab-null animals at 6 weeks (a–d) or transplanted WT or K6ab-null tissues were harvested after either 4 weeks of outgrowth (e–h) or 10 weeks of outgrowth after 2 days of treatment with estrogen and progesterone (i–l). Whole-mount staining of the glands to reveal ductal structures is shown at two magnifications. Scale bar, 1 mm. LN, lymph node. |
PMC1557774_pgen-0020112-g001_6899.jpg | What is shown in this image? | Mammary Anlagen(A) Mammary anlagen 3 and 4 are visible at E10.75/E11.0 (38–40 somites) in histological sagittal sections of mouse embryos stained with hematoxylin.FL, forelimb bud; HL, hindlimb bud; MLM, mammary line mesenchyme; NT, neural tube(B) Higher magnification of mammary region from (A). Anlagen 3 and 4 at higher magnification. |
PMC1557774_pgen-0020112-g001_6897.jpg | What object or scene is depicted here? | Mammary Anlagen(A) Mammary anlagen 3 and 4 are visible at E10.75/E11.0 (38–40 somites) in histological sagittal sections of mouse embryos stained with hematoxylin.FL, forelimb bud; HL, hindlimb bud; MLM, mammary line mesenchyme; NT, neural tube(B) Higher magnification of mammary region from (A). Anlagen 3 and 4 at higher magnification. |
PMC1557774_pgen-0020112-g001_6898.jpg | What is the focal point of this photograph? | Mammary Anlagen(A) Mammary anlagen 3 and 4 are visible at E10.75/E11.0 (38–40 somites) in histological sagittal sections of mouse embryos stained with hematoxylin.FL, forelimb bud; HL, hindlimb bud; MLM, mammary line mesenchyme; NT, neural tube(B) Higher magnification of mammary region from (A). Anlagen 3 and 4 at higher magnification. |
PMC1557784_pgen-0020136-g006_6906.jpg | What is shown in this image? | Immunolocalization of SCP1 (Green), Centromeres (Red), and Staining of the Chromatin with DAPI (Blue)Several focal planes are superimposed and projected in a single plane in each picture.(A–C) Pachytene. SCP1 appears as continuous lines along autosomes and is completely absent on the sex chromosomes. (A' and C'). Detailed view of the sex chromosomes in the same cell.(D–F) Diplotene. SCP1 mostly appears as fragmented lines that run along chromosomes. These lines are continuous in some regions. SCP1 remains associated at the centromeric regions of autosomes and also appears associated to the X chromosome centromere (arrowheads). A structure labeled with SCP1 appears at the periphery of the sex body (arrow). (D' and F') At a higher magnification this structure is clearly detected near the centromere of the Y chromosome.(G–I) Prometaphase I. SCP1 signal has completely disappeared from the chromosomes. However, a small structure is clearly labeled (arrow). Bivalents are not oriented at the metaphase plate. Sex chromosomes (X, Y) are clearly discernible, and they remain associated. (G' and I'). Enlargement of the sex chromosomes. The SCP1-labeled structure appears at the region of contact between sex chromosomes (arrow).(J–L) Metaphase I. The SCP1-labeled structure is present in the region where sex chromosomes (X, Y) are located. (J'–L') At a higher magnification it can be seen that the SCP1-labeled structure (arrow) is associated to the sex chromosomes.(M–O). Anaphase I. Homologous chromosomes have segregated and the SCP1-labeled structure is still visible (arrow). However, it does not maintain any association with the chromatin masses near the cell poles. Bar: 5 μm in (A–O); 1 μm in (A'–L'). |
PMC1557784_pgen-0020136-g006_6901.jpg | Can you identify the primary element in this image? | Immunolocalization of SCP1 (Green), Centromeres (Red), and Staining of the Chromatin with DAPI (Blue)Several focal planes are superimposed and projected in a single plane in each picture.(A–C) Pachytene. SCP1 appears as continuous lines along autosomes and is completely absent on the sex chromosomes. (A' and C'). Detailed view of the sex chromosomes in the same cell.(D–F) Diplotene. SCP1 mostly appears as fragmented lines that run along chromosomes. These lines are continuous in some regions. SCP1 remains associated at the centromeric regions of autosomes and also appears associated to the X chromosome centromere (arrowheads). A structure labeled with SCP1 appears at the periphery of the sex body (arrow). (D' and F') At a higher magnification this structure is clearly detected near the centromere of the Y chromosome.(G–I) Prometaphase I. SCP1 signal has completely disappeared from the chromosomes. However, a small structure is clearly labeled (arrow). Bivalents are not oriented at the metaphase plate. Sex chromosomes (X, Y) are clearly discernible, and they remain associated. (G' and I'). Enlargement of the sex chromosomes. The SCP1-labeled structure appears at the region of contact between sex chromosomes (arrow).(J–L) Metaphase I. The SCP1-labeled structure is present in the region where sex chromosomes (X, Y) are located. (J'–L') At a higher magnification it can be seen that the SCP1-labeled structure (arrow) is associated to the sex chromosomes.(M–O). Anaphase I. Homologous chromosomes have segregated and the SCP1-labeled structure is still visible (arrow). However, it does not maintain any association with the chromatin masses near the cell poles. Bar: 5 μm in (A–O); 1 μm in (A'–L'). |
PMC1557784_pgen-0020136-g006_6902.jpg | What is the dominant medical problem in this image? | Immunolocalization of SCP1 (Green), Centromeres (Red), and Staining of the Chromatin with DAPI (Blue)Several focal planes are superimposed and projected in a single plane in each picture.(A–C) Pachytene. SCP1 appears as continuous lines along autosomes and is completely absent on the sex chromosomes. (A' and C'). Detailed view of the sex chromosomes in the same cell.(D–F) Diplotene. SCP1 mostly appears as fragmented lines that run along chromosomes. These lines are continuous in some regions. SCP1 remains associated at the centromeric regions of autosomes and also appears associated to the X chromosome centromere (arrowheads). A structure labeled with SCP1 appears at the periphery of the sex body (arrow). (D' and F') At a higher magnification this structure is clearly detected near the centromere of the Y chromosome.(G–I) Prometaphase I. SCP1 signal has completely disappeared from the chromosomes. However, a small structure is clearly labeled (arrow). Bivalents are not oriented at the metaphase plate. Sex chromosomes (X, Y) are clearly discernible, and they remain associated. (G' and I'). Enlargement of the sex chromosomes. The SCP1-labeled structure appears at the region of contact between sex chromosomes (arrow).(J–L) Metaphase I. The SCP1-labeled structure is present in the region where sex chromosomes (X, Y) are located. (J'–L') At a higher magnification it can be seen that the SCP1-labeled structure (arrow) is associated to the sex chromosomes.(M–O). Anaphase I. Homologous chromosomes have segregated and the SCP1-labeled structure is still visible (arrow). However, it does not maintain any association with the chromatin masses near the cell poles. Bar: 5 μm in (A–O); 1 μm in (A'–L'). |
PMC1557784_pgen-0020136-g006_6913.jpg | What is the focal point of this photograph? | Immunolocalization of SCP1 (Green), Centromeres (Red), and Staining of the Chromatin with DAPI (Blue)Several focal planes are superimposed and projected in a single plane in each picture.(A–C) Pachytene. SCP1 appears as continuous lines along autosomes and is completely absent on the sex chromosomes. (A' and C'). Detailed view of the sex chromosomes in the same cell.(D–F) Diplotene. SCP1 mostly appears as fragmented lines that run along chromosomes. These lines are continuous in some regions. SCP1 remains associated at the centromeric regions of autosomes and also appears associated to the X chromosome centromere (arrowheads). A structure labeled with SCP1 appears at the periphery of the sex body (arrow). (D' and F') At a higher magnification this structure is clearly detected near the centromere of the Y chromosome.(G–I) Prometaphase I. SCP1 signal has completely disappeared from the chromosomes. However, a small structure is clearly labeled (arrow). Bivalents are not oriented at the metaphase plate. Sex chromosomes (X, Y) are clearly discernible, and they remain associated. (G' and I'). Enlargement of the sex chromosomes. The SCP1-labeled structure appears at the region of contact between sex chromosomes (arrow).(J–L) Metaphase I. The SCP1-labeled structure is present in the region where sex chromosomes (X, Y) are located. (J'–L') At a higher magnification it can be seen that the SCP1-labeled structure (arrow) is associated to the sex chromosomes.(M–O). Anaphase I. Homologous chromosomes have segregated and the SCP1-labeled structure is still visible (arrow). However, it does not maintain any association with the chromatin masses near the cell poles. Bar: 5 μm in (A–O); 1 μm in (A'–L'). |
PMC1557784_pgen-0020136-g008_6918.jpg | What is being portrayed in this visual content? | Triple Immunolocalization of SCP3 (Green), SCP1 (Red), and Centromeres (Red), and Chromatin Staining with DAPI (Blue) in a Spermatocyte at Metaphase ITwo focal planes are superimposed in each picture.(A) Immunolocalization of SCP1 (red) and DAPI counterstaining (blue) of a spermatocyte in metaphase I. A small structure associated with the chromatin appears labeled (arrow).(B) Immunolocalization of SCP3 (green) and centromeres (red) on the same spermatocyte shown in (A). SCP3 appears on the three bivalents shown in these focal planes. The labeling runs on the interchromatid domain and interrupts at the chiasma sites (arrowheads). The SCP3 labeling allows the identification of the sex chromosomes (X, Y) and the DP. Sex chromosomes have already initiated their segregation and have lost contact.(C) Merge of the images (A) and (B) reveals that the structure labeled with anti-SCP1 is the DP, which in this spermatocyte has remained associated with the Y chromosome.(D) Detail of the sex chromosomes.(E) Schematic representation of sex chromosomes and the DP.Bar: 5 μm in (A–C); 1 μm in (D). |
PMC1557784_pgen-0020136-g008_6919.jpg | What is the dominant medical problem in this image? | Triple Immunolocalization of SCP3 (Green), SCP1 (Red), and Centromeres (Red), and Chromatin Staining with DAPI (Blue) in a Spermatocyte at Metaphase ITwo focal planes are superimposed in each picture.(A) Immunolocalization of SCP1 (red) and DAPI counterstaining (blue) of a spermatocyte in metaphase I. A small structure associated with the chromatin appears labeled (arrow).(B) Immunolocalization of SCP3 (green) and centromeres (red) on the same spermatocyte shown in (A). SCP3 appears on the three bivalents shown in these focal planes. The labeling runs on the interchromatid domain and interrupts at the chiasma sites (arrowheads). The SCP3 labeling allows the identification of the sex chromosomes (X, Y) and the DP. Sex chromosomes have already initiated their segregation and have lost contact.(C) Merge of the images (A) and (B) reveals that the structure labeled with anti-SCP1 is the DP, which in this spermatocyte has remained associated with the Y chromosome.(D) Detail of the sex chromosomes.(E) Schematic representation of sex chromosomes and the DP.Bar: 5 μm in (A–C); 1 μm in (D). |
PMC1557797_F2_6922.jpg | What is being portrayed in this visual content? | MRI (coronal view) showed the tumor in the right
infratemporal fossa with intracranial extension and invasion of
the maxillary sinus and mandible. |
PMC1557838_F6_6931.jpg | What is the dominant medical problem in this image? | A Stat1-dependent host response restricts the spread of HSV-1 strain KOS-GFP into the central nervous system. Strain 129 mice, rag2-/- mice, stat1-/- mice, or rag2-/- stat1-/- mice were inoculated with 2 × 105 pfu per eye of HSV-1 strain KOS-GFP. A. The mean ± sem of the logarithm of viral titers recovered from homogenates of mouse eyes, TG, and hindbrain is plotted as a function of the time p.i. at which tissues were harvested (n = 5 per time point). Asterisks denote significant differences between stat1+/+ versus stat1-/- tissues (p < 0.001, as determined by two-way ANOVA). Dashed lines indicate the lower limit of detection of each plaque assay. B. GFP expression in tissues of KOS-GFP-infected mice. Representative photographs are shown of eyes harvested on day 3 p.i. (4× magnification, 250 ms exposure), TG harvested on day 5 p.i. (2× magnification, 500 ms exposure), and the ventral side of brains harvested on day 7 p.i. (2× magnification, 1000 ms exposure). |
PMC1557838_F6_6932.jpg | What is the central feature of this picture? | A Stat1-dependent host response restricts the spread of HSV-1 strain KOS-GFP into the central nervous system. Strain 129 mice, rag2-/- mice, stat1-/- mice, or rag2-/- stat1-/- mice were inoculated with 2 × 105 pfu per eye of HSV-1 strain KOS-GFP. A. The mean ± sem of the logarithm of viral titers recovered from homogenates of mouse eyes, TG, and hindbrain is plotted as a function of the time p.i. at which tissues were harvested (n = 5 per time point). Asterisks denote significant differences between stat1+/+ versus stat1-/- tissues (p < 0.001, as determined by two-way ANOVA). Dashed lines indicate the lower limit of detection of each plaque assay. B. GFP expression in tissues of KOS-GFP-infected mice. Representative photographs are shown of eyes harvested on day 3 p.i. (4× magnification, 250 ms exposure), TG harvested on day 5 p.i. (2× magnification, 500 ms exposure), and the ventral side of brains harvested on day 7 p.i. (2× magnification, 1000 ms exposure). |
PMC1557838_F6_6933.jpg | What is the dominant medical problem in this image? | A Stat1-dependent host response restricts the spread of HSV-1 strain KOS-GFP into the central nervous system. Strain 129 mice, rag2-/- mice, stat1-/- mice, or rag2-/- stat1-/- mice were inoculated with 2 × 105 pfu per eye of HSV-1 strain KOS-GFP. A. The mean ± sem of the logarithm of viral titers recovered from homogenates of mouse eyes, TG, and hindbrain is plotted as a function of the time p.i. at which tissues were harvested (n = 5 per time point). Asterisks denote significant differences between stat1+/+ versus stat1-/- tissues (p < 0.001, as determined by two-way ANOVA). Dashed lines indicate the lower limit of detection of each plaque assay. B. GFP expression in tissues of KOS-GFP-infected mice. Representative photographs are shown of eyes harvested on day 3 p.i. (4× magnification, 250 ms exposure), TG harvested on day 5 p.i. (2× magnification, 500 ms exposure), and the ventral side of brains harvested on day 7 p.i. (2× magnification, 1000 ms exposure). |
PMC1557838_F7_6925.jpg | What key item or scene is captured in this photo? | Loss of IFN-α/β receptors or Stat 1 impairs an innate host response that represses KOS-GFP and 0--GFP at the site of inoculation. Mice were inoculated with 2 × 105 pfu per eye of A. HSV-1 strain KOS-GFP, or B. the ICP0- virus, 0--GFP. GFP fluorescence was recorded in the right eyes of strain 129 mice, rag2-/- mice (lymphocyte-deficient), ifngr-/- mice (IFN-γ receptor-null), ifnar-/- mice (IFN-α/β receptor-null), ifnar-/- ifngr-/- mice, stat1-/- mice, rag2-/- stat1-/- mice, and rag2-/- ifnar-/- mice. Representative photographs are shown of GFP fluorescence in the virus-infected eye of one mouse per group photographed over time at 36, 60, and 84 hours p.i. (4× magnification; 39 ms exposure for KOS-GFP; 63 ms exposure for 0--GFP). |
PMC1557838_F7_6924.jpg | What is the principal component of this image? | Loss of IFN-α/β receptors or Stat 1 impairs an innate host response that represses KOS-GFP and 0--GFP at the site of inoculation. Mice were inoculated with 2 × 105 pfu per eye of A. HSV-1 strain KOS-GFP, or B. the ICP0- virus, 0--GFP. GFP fluorescence was recorded in the right eyes of strain 129 mice, rag2-/- mice (lymphocyte-deficient), ifngr-/- mice (IFN-γ receptor-null), ifnar-/- mice (IFN-α/β receptor-null), ifnar-/- ifngr-/- mice, stat1-/- mice, rag2-/- stat1-/- mice, and rag2-/- ifnar-/- mice. Representative photographs are shown of GFP fluorescence in the virus-infected eye of one mouse per group photographed over time at 36, 60, and 84 hours p.i. (4× magnification; 39 ms exposure for KOS-GFP; 63 ms exposure for 0--GFP). |
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