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PMC1550268_ppat-0020085-g003_6616.jpg | Can you identify the primary element in this image? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6614.jpg | What is the main focus of this visual representation? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6615.jpg | What is the central feature of this picture? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6617.jpg | What is being portrayed in this visual content? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6618.jpg | What can you see in this picture? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6625.jpg | What does this image primarily show? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6619.jpg | What is the central feature of this picture? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g003_6621.jpg | What object or scene is depicted here? | Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the CellNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body. |
PMC1550268_ppat-0020085-g004_6633.jpg | Describe the main subject of this image. | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6637.jpg | Can you identify the primary element in this image? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6629.jpg | What is the dominant medical problem in this image? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6644.jpg | Can you identify the primary element in this image? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6635.jpg | What object or scene is depicted here? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6627.jpg | What is shown in this image? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6630.jpg | What can you see in this picture? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6638.jpg | What stands out most in this visual? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6643.jpg | What is shown in this image? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6640.jpg | What stands out most in this visual? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6631.jpg | What is being portrayed in this visual content? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6626.jpg | What does this image primarily show? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6632.jpg | What is the central feature of this picture? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g004_6634.jpg | What is the main focus of this visual representation? | Time Course of Actin Filament Formation and Capsid Assembly in the Nucleus(A) SCG neurons were infected with PRV expressing GFP-VP26 at fixed times shown. The inset at 6 hpi is an enlargement of the nucleus from the cell on the right, which has small nuclear actin filaments. The brightness of the inset image has been enhanced in order to more clearly visualize the filaments. The arrowheads indicate actin filaments that appear to emanate from the nuclear envelope at 9 hpi. A single focal plane through the nucleus is shown. Scale bar = 20 μm.(B) Chart showing the relative formation of nuclear actin filaments, the presence of GFP-VP26 fluorescence, and emergence of GFP-VP26 foci over the course of infection. |
PMC1550268_ppat-0020085-g006_6660.jpg | What is the main focus of this visual representation? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6664.jpg | Can you identify the primary element in this image? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6667.jpg | What is the principal component of this image? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6655.jpg | What is the core subject represented in this visual? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6663.jpg | What object or scene is depicted here? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6659.jpg | What object or scene is depicted here? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6658.jpg | What's the most prominent thing you notice in this picture? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6657.jpg | What is the focal point of this photograph? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6665.jpg | What is the central feature of this picture? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6656.jpg | What is the focal point of this photograph? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6661.jpg | What is the core subject represented in this visual? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550268_ppat-0020085-g006_6662.jpg | What is the focal point of this photograph? | Drug Effects on Actin Filament Formation and Capsid Assembly Organization in the NucleusInfected cells were treated with latA, jasp, or DMSO as indicated.(A) Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm. The contrast of the α-LAP2 signal has been enhanced in jasp-treated cells.(B) TEM of infected cells treated with with latA, jasp, or DMSO as indicated. Asterisks indicate capsid assemblies; arrows point out individual capsids. Inset shows filaments from a different jasp-treated cell; arrows point to filaments. Scale bar = 2 μm. |
PMC1550409_F2_6668.jpg | What is the dominant medical problem in this image? | CT scan showing multiple areas of pulmonary infarction. |
PMC1550409_F2_6669.jpg | What does this image primarily show? | CT scan showing multiple areas of pulmonary infarction. |
PMC1550415_F1_6670.jpg | Describe the main subject of this image. | Micrographs of immunocytochemically labeled coronal sections of female P. shermani vomeronasal epithelium (VNE) demonstrating agmatine uptake by stimulation with PRF (A) or a saline control solution (B). (A) Application of PRF resulted in the labeling of numerous vomeronasal receptor neurons (arrow head illustrates one of the labeled cell bodies) throughout all depths of the epithelial layer. Labeling extended from the dendritic knob at the epithelial surface (small arrow) to the proximal portion of the axon (large arrow) within the organ. PRF application resulted in moderate-to-heavy intensity of labeling. (B) Application of saline produced very few labeled vomeronasal receptor neurons (arrow head) and these were very lightly labeled. Bars = 40 μm. |
PMC1550415_F5_6673.jpg | What's the most prominent thing you notice in this picture? | Micrographs of immunocytochemically labeled coronal sections of female P. shermani vomeronasal epithelium (VNE) demonstrating agmatine uptake by stimulation with PMF. (A) or a saline control solution (B). (A) Application of PMF resulted in the labeling of a moderate number of vomeronasal receptor neurons (arrow head) throughout all depths of the epithelial layer. Labeling of vomeronasal neurons by PMF was less intense than that by PRF. (B) Application of saline produced very few lightly labeled vomeronasal receptor neurons. Bars = 40 μm. |
PMC1550421_F4_6674.jpg | What is the core subject represented in this visual? | Positive ER staining confirming the breast origin of the metastasis. (original magnification ×200). |
PMC1550422_F2_6677.jpg | What can you see in this picture? | Immunohistochemical findings in breast, ovarian, and cervical cancer and malignant melanoma, and corresponding benign tissue. (magnification 250×) a) Normal breast tissue with no detectable expression of the TRAP protein by immunohistochemistry. The brown staining in the duct is due to nonspecific staining of secretions. b) Invasive ductal carcinoma of the breast with significant expression of TRAP protein. (brown = DAB) c) Benign ovarian celomic epithelium with no expression of TRAP. d) Serous papillary ovarian cancer cells expressing TRAP (brown = DAB). e) No TRAP expression is detectable in non-transformed cervical tissue. f) Only weak expression of TRAP is seen in this cervical cancer specimen. Tumor-infiltrating monocytes clearly stain for TRAP, as shown in the enlarged section (brown = DAB). g) No expression of TRAP is detectable by immunohistochemistry in normal skin (VIP). The slight brown staining is due to skin pigmentation. h) There is marked overexpression of TRAP in the malignant melanoma in the same specimen as in (g), allowing differentiation of the malignant tumor from the adjacent normal skin. The slight brown staining is due to skin pigmentation. (purple = VIP) |
PMC1550422_F2_6675.jpg | What is the main focus of this visual representation? | Immunohistochemical findings in breast, ovarian, and cervical cancer and malignant melanoma, and corresponding benign tissue. (magnification 250×) a) Normal breast tissue with no detectable expression of the TRAP protein by immunohistochemistry. The brown staining in the duct is due to nonspecific staining of secretions. b) Invasive ductal carcinoma of the breast with significant expression of TRAP protein. (brown = DAB) c) Benign ovarian celomic epithelium with no expression of TRAP. d) Serous papillary ovarian cancer cells expressing TRAP (brown = DAB). e) No TRAP expression is detectable in non-transformed cervical tissue. f) Only weak expression of TRAP is seen in this cervical cancer specimen. Tumor-infiltrating monocytes clearly stain for TRAP, as shown in the enlarged section (brown = DAB). g) No expression of TRAP is detectable by immunohistochemistry in normal skin (VIP). The slight brown staining is due to skin pigmentation. h) There is marked overexpression of TRAP in the malignant melanoma in the same specimen as in (g), allowing differentiation of the malignant tumor from the adjacent normal skin. The slight brown staining is due to skin pigmentation. (purple = VIP) |
PMC1550422_F2_6682.jpg | Describe the main subject of this image. | Immunohistochemical findings in breast, ovarian, and cervical cancer and malignant melanoma, and corresponding benign tissue. (magnification 250×) a) Normal breast tissue with no detectable expression of the TRAP protein by immunohistochemistry. The brown staining in the duct is due to nonspecific staining of secretions. b) Invasive ductal carcinoma of the breast with significant expression of TRAP protein. (brown = DAB) c) Benign ovarian celomic epithelium with no expression of TRAP. d) Serous papillary ovarian cancer cells expressing TRAP (brown = DAB). e) No TRAP expression is detectable in non-transformed cervical tissue. f) Only weak expression of TRAP is seen in this cervical cancer specimen. Tumor-infiltrating monocytes clearly stain for TRAP, as shown in the enlarged section (brown = DAB). g) No expression of TRAP is detectable by immunohistochemistry in normal skin (VIP). The slight brown staining is due to skin pigmentation. h) There is marked overexpression of TRAP in the malignant melanoma in the same specimen as in (g), allowing differentiation of the malignant tumor from the adjacent normal skin. The slight brown staining is due to skin pigmentation. (purple = VIP) |
PMC1550422_F2_6680.jpg | What is being portrayed in this visual content? | Immunohistochemical findings in breast, ovarian, and cervical cancer and malignant melanoma, and corresponding benign tissue. (magnification 250×) a) Normal breast tissue with no detectable expression of the TRAP protein by immunohistochemistry. The brown staining in the duct is due to nonspecific staining of secretions. b) Invasive ductal carcinoma of the breast with significant expression of TRAP protein. (brown = DAB) c) Benign ovarian celomic epithelium with no expression of TRAP. d) Serous papillary ovarian cancer cells expressing TRAP (brown = DAB). e) No TRAP expression is detectable in non-transformed cervical tissue. f) Only weak expression of TRAP is seen in this cervical cancer specimen. Tumor-infiltrating monocytes clearly stain for TRAP, as shown in the enlarged section (brown = DAB). g) No expression of TRAP is detectable by immunohistochemistry in normal skin (VIP). The slight brown staining is due to skin pigmentation. h) There is marked overexpression of TRAP in the malignant melanoma in the same specimen as in (g), allowing differentiation of the malignant tumor from the adjacent normal skin. The slight brown staining is due to skin pigmentation. (purple = VIP) |
PMC1550422_F2_6676.jpg | What is shown in this image? | Immunohistochemical findings in breast, ovarian, and cervical cancer and malignant melanoma, and corresponding benign tissue. (magnification 250×) a) Normal breast tissue with no detectable expression of the TRAP protein by immunohistochemistry. The brown staining in the duct is due to nonspecific staining of secretions. b) Invasive ductal carcinoma of the breast with significant expression of TRAP protein. (brown = DAB) c) Benign ovarian celomic epithelium with no expression of TRAP. d) Serous papillary ovarian cancer cells expressing TRAP (brown = DAB). e) No TRAP expression is detectable in non-transformed cervical tissue. f) Only weak expression of TRAP is seen in this cervical cancer specimen. Tumor-infiltrating monocytes clearly stain for TRAP, as shown in the enlarged section (brown = DAB). g) No expression of TRAP is detectable by immunohistochemistry in normal skin (VIP). The slight brown staining is due to skin pigmentation. h) There is marked overexpression of TRAP in the malignant melanoma in the same specimen as in (g), allowing differentiation of the malignant tumor from the adjacent normal skin. The slight brown staining is due to skin pigmentation. (purple = VIP) |
PMC1550422_F2_6681.jpg | What is the main focus of this visual representation? | Immunohistochemical findings in breast, ovarian, and cervical cancer and malignant melanoma, and corresponding benign tissue. (magnification 250×) a) Normal breast tissue with no detectable expression of the TRAP protein by immunohistochemistry. The brown staining in the duct is due to nonspecific staining of secretions. b) Invasive ductal carcinoma of the breast with significant expression of TRAP protein. (brown = DAB) c) Benign ovarian celomic epithelium with no expression of TRAP. d) Serous papillary ovarian cancer cells expressing TRAP (brown = DAB). e) No TRAP expression is detectable in non-transformed cervical tissue. f) Only weak expression of TRAP is seen in this cervical cancer specimen. Tumor-infiltrating monocytes clearly stain for TRAP, as shown in the enlarged section (brown = DAB). g) No expression of TRAP is detectable by immunohistochemistry in normal skin (VIP). The slight brown staining is due to skin pigmentation. h) There is marked overexpression of TRAP in the malignant melanoma in the same specimen as in (g), allowing differentiation of the malignant tumor from the adjacent normal skin. The slight brown staining is due to skin pigmentation. (purple = VIP) |
PMC1550428_F3_6683.jpg | What can you see in this picture? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6688.jpg | What's the most prominent thing you notice in this picture? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6690.jpg | Can you identify the primary element in this image? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6686.jpg | What can you see in this picture? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6684.jpg | Can you identify the primary element in this image? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6691.jpg | What key item or scene is captured in this photo? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6695.jpg | What is the focal point of this photograph? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6696.jpg | What is the focal point of this photograph? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550428_F3_6697.jpg | What is the main focus of this visual representation? | Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison. |
PMC1550558_figure2_6700.jpg | What is the principal component of this image? | Two examples of the 10 breast needle core biopsies presented to 17 pathologists or trainee pathologists using the VPS (for all 10 images, see Multimedia Appendix 3) |
PMC1550895_F1_6702.jpg | What is the core subject represented in this visual? | Wound management via vacuum-assisted closure therapy. (a) Large-scale tissue damage at hip and upper lower extremity. (b) Vacuum-assisted closure therapy. (c) Successful skin grafting. |
PMC1550895_F1_6703.jpg | Can you identify the primary element in this image? | Wound management via vacuum-assisted closure therapy. (a) Large-scale tissue damage at hip and upper lower extremity. (b) Vacuum-assisted closure therapy. (c) Successful skin grafting. |
PMC1550895_F1_6701.jpg | What is the dominant medical problem in this image? | Wound management via vacuum-assisted closure therapy. (a) Large-scale tissue damage at hip and upper lower extremity. (b) Vacuum-assisted closure therapy. (c) Successful skin grafting. |
PMC1550895_F6_6705.jpg | What key item or scene is captured in this photo? | Computed cranial tomography (CCT): Arrows indicate fluid and opaque material in the (a) ethmoid and (b) maxillary sinuses. |
PMC1551923_pbio-0040284-g004_6719.jpg | What is shown in this image? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6715.jpg | What is the core subject represented in this visual? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6713.jpg | What is being portrayed in this visual content? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6717.jpg | What's the most prominent thing you notice in this picture? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6716.jpg | What object or scene is depicted here? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6710.jpg | What is being portrayed in this visual content? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6712.jpg | What stands out most in this visual? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1551923_pbio-0040284-g004_6709.jpg | What is shown in this image? | Identification of the Pulmonary and Hepatic Cells Producing PGE2 at the Onset of LPS Fever in RatsImmunolocalization of COX-2 in the lung and liver and identification of the cell types expressing this enzyme. Top row: tissue localization of COX-2 (green immunofluorescence) in the lung and liver of rats at 40 min after i.v. injection of saline or at the onset of the first febrile phase (i.e., 40 min after i.v. injection of LPS, 10 μg/kg) at thermoneutrality. Next rows: dual localization of LPS-induced COX-2–immunoreactivity (green; left column) with either the macrophage marker ED2 or the endothelial cell marker RECA1 (red; middle column) in the lung and liver at the onset of the first febrile phase. Doubly labeled cells appear yellow in the merged confocal images (right column). White arrows and black arrowheads mark examples of doubly and singly (COX-2 only) labeled cells, respectively. Scale bars represent 40 μm. |
PMC1552053_F1_6722.jpg | What object or scene is depicted here? | Chest radiograph showing soft tissue mass within the right hemithorax (A). CT (B) demonstrates that the mass contains low attenuation soft tissue suggestive of fat, and calcification, features in keeping with a teratoma. |
PMC1552053_F1_6721.jpg | Can you identify the primary element in this image? | Chest radiograph showing soft tissue mass within the right hemithorax (A). CT (B) demonstrates that the mass contains low attenuation soft tissue suggestive of fat, and calcification, features in keeping with a teratoma. |
PMC1552057_F2_6727.jpg | What does this image primarily show? | Expression of activated DRok induces eye and wing defects. (A-C) Scanning Electron Microscopy photographs of wild-type (A), one-copy GMR-Drok-cat transgenic (B) or two-copy GMR-Drok-cat transgenic (C) eyes. Expression of one copy of DRok-cat results in a slightly rough eye (B), of two copies in a rougher eye phenotype (C), which provides a basis for a convenient assay to identify genetic modifiers. (D-F) Tangential retinal sections of wild-type (D), one-copy GMR-Drok-cat transgenic (E) or one-copy GMR-Drok-cat and one-copy GMR-p35 (F) eyes. In the one copy-transgenic eye, the underlying retina is severely disrupted, associated with loss of cells (E, arrow). This phenotype can be rescued by overexpressing the baculoviral caspase inhibitor p35 (F). (G, H) Light microscopy photographs of a wild-type (G) or a en-GAL4<UAS-Drok-cat expressing (H) wing. Expression of DRok-cat in the posterior part of the wing results in the disappearance of the crossveins (H, arrows). |
PMC1552057_F2_6726.jpg | Can you identify the primary element in this image? | Expression of activated DRok induces eye and wing defects. (A-C) Scanning Electron Microscopy photographs of wild-type (A), one-copy GMR-Drok-cat transgenic (B) or two-copy GMR-Drok-cat transgenic (C) eyes. Expression of one copy of DRok-cat results in a slightly rough eye (B), of two copies in a rougher eye phenotype (C), which provides a basis for a convenient assay to identify genetic modifiers. (D-F) Tangential retinal sections of wild-type (D), one-copy GMR-Drok-cat transgenic (E) or one-copy GMR-Drok-cat and one-copy GMR-p35 (F) eyes. In the one copy-transgenic eye, the underlying retina is severely disrupted, associated with loss of cells (E, arrow). This phenotype can be rescued by overexpressing the baculoviral caspase inhibitor p35 (F). (G, H) Light microscopy photographs of a wild-type (G) or a en-GAL4<UAS-Drok-cat expressing (H) wing. Expression of DRok-cat in the posterior part of the wing results in the disappearance of the crossveins (H, arrows). |
PMC1552057_F2_6723.jpg | What stands out most in this visual? | Expression of activated DRok induces eye and wing defects. (A-C) Scanning Electron Microscopy photographs of wild-type (A), one-copy GMR-Drok-cat transgenic (B) or two-copy GMR-Drok-cat transgenic (C) eyes. Expression of one copy of DRok-cat results in a slightly rough eye (B), of two copies in a rougher eye phenotype (C), which provides a basis for a convenient assay to identify genetic modifiers. (D-F) Tangential retinal sections of wild-type (D), one-copy GMR-Drok-cat transgenic (E) or one-copy GMR-Drok-cat and one-copy GMR-p35 (F) eyes. In the one copy-transgenic eye, the underlying retina is severely disrupted, associated with loss of cells (E, arrow). This phenotype can be rescued by overexpressing the baculoviral caspase inhibitor p35 (F). (G, H) Light microscopy photographs of a wild-type (G) or a en-GAL4<UAS-Drok-cat expressing (H) wing. Expression of DRok-cat in the posterior part of the wing results in the disappearance of the crossveins (H, arrows). |
PMC1552057_F2_6730.jpg | What's the most prominent thing you notice in this picture? | Expression of activated DRok induces eye and wing defects. (A-C) Scanning Electron Microscopy photographs of wild-type (A), one-copy GMR-Drok-cat transgenic (B) or two-copy GMR-Drok-cat transgenic (C) eyes. Expression of one copy of DRok-cat results in a slightly rough eye (B), of two copies in a rougher eye phenotype (C), which provides a basis for a convenient assay to identify genetic modifiers. (D-F) Tangential retinal sections of wild-type (D), one-copy GMR-Drok-cat transgenic (E) or one-copy GMR-Drok-cat and one-copy GMR-p35 (F) eyes. In the one copy-transgenic eye, the underlying retina is severely disrupted, associated with loss of cells (E, arrow). This phenotype can be rescued by overexpressing the baculoviral caspase inhibitor p35 (F). (G, H) Light microscopy photographs of a wild-type (G) or a en-GAL4<UAS-Drok-cat expressing (H) wing. Expression of DRok-cat in the posterior part of the wing results in the disappearance of the crossveins (H, arrows). |
PMC1552057_F4_6731.jpg | What's the most prominent thing you notice in this picture? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F4_6739.jpg | What is shown in this image? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F4_6736.jpg | What is the core subject represented in this visual? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F4_6732.jpg | What is being portrayed in this visual content? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F4_6733.jpg | What object or scene is depicted here? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F4_6740.jpg | What is the principal component of this image? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F4_6737.jpg | Can you identify the primary element in this image? | zipper as a genetic interactor of Drok in a screen for dominant suppressors of the DRok-cat expression-induced rough eye phenotype. (A-C) Expression of two copies of the GMR-Drok-cat transgene (GMR-Drok-cat1, GMR-Drok-cat2) induces a rough eye phenotype associated with a smaller eye size (B) compared to a wild-type eye (A). This phenotype was dominantly suppressed by four independent EMS-induced mutations which all map to the zipper locus, the Drosophila non-muscle myosin heavy chain gene. The loss-of-function zip1 mutation also rescued the GMR-Drok-cat1, GMR-Drok-cat2-induced eye phenotype (C). (D-G) Tangential retinal sections of eyes of the following genotype: wild-type (D), one-copy GMR-Drok-cat transgenic (E), or one-copy GMR-Drok-cat and one mutant loss-of-function allele of either zipper (zip1) or spaghetti squash (sqh2) (F or G, respectively). Unlike zip1, sqh2, a loss-of-function mutant of the Drosophila non-muscle myosin light chain, does not suppress the GMR-Drok-cat-induced eye phenotype. (H-J) Light microscopy photographs of a wild-type wing (H), a wing expressing en-GAL4<UAS-Drok-cat with missing crossveins (I, arrow) or a wing expressing en-GAL4<UAS-Drok-cat in a heterozygous zip1 background (J). Heterozygosity for zipper did partially rescue the missing crossvein phenotype (J, arrow). |
PMC1552057_F5_6745.jpg | What is the dominant medical problem in this image? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6741.jpg | What key item or scene is captured in this photo? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6744.jpg | What is being portrayed in this visual content? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6743.jpg | What is the focal point of this photograph? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6747.jpg | Describe the main subject of this image. | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6750.jpg | What is the main focus of this visual representation? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6753.jpg | Can you identify the primary element in this image? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552057_F5_6749.jpg | What is being portrayed in this visual content? | DRok in axonal development. (A, B) Immunostaining of either wild-type (A) or DRok-cat-expressing (B) photoreceptor neurons which send axonal projections from the developing 3rd instar larval eye disc into the optic lobe of the brain. Axonal guidance and targeting appear normal in DRok-cat-expressing larval eye discs. GMR-Drok-cat/+ photoreceptors project correctly to the lamina and medulla layers into the optic lobe. In B, the axons are folded due to tissue mounting. (C-H) Double immunostaining of either wild-type (C-E), or GMR-Drok-cat/+ (F-H) 3rd instar larval eye discs, with phalloidin (C, F) and an anti-Elav antibody (D, G) to detect actin and differentiating neurons, respectively. The overall morphology and differentiation pattern in photoreceptors is undistinguishable between wild-type and DRok-cat-expressing 3rd instar larval eye discs. (I-L) Scanning electron microscopy pictures of wild-type (I), GMR-Dlimk/+ (J), GMR-Drok-cat/+ (K) or GMR-Dlimk/GMR-Drok-cat (L) eyes. Whereas overexpression of DLimk or expression of DRok-cat, separately, does not perturb the external morphology of the eye, co-expression of DLimk and DRok-cat results in a strong rough eye phenotype associated with decreased eye size. (M-P) Immunostaining of the embryonic CNS (BP102 antibody) of the following genotypes: wild-type (M), elav-Gal4>UAS-Drok-cat (N), elav-Gal4>UAS-Dlimk (O) or elav-Gal4>UAS-Drok-cat, UAS-Dlimk (P). As observed for the eyes, whereas overexpression of DLimk or expression of DRok-cat alone does not alter the proper organization of the embryonic CNS marked by adjacent patterns of connected neurons (M), co-expression of DLimk and DRok-cat leads to the disruption of connecting neurons (P, arrow). |
PMC1552085_F1_6755.jpg | What is the principal component of this image? | Immunohistochemical detection of AFP in progenitor cells. Immunohistochemical detection of AFP in progenitor cells (oval cells) in the rat liver. The right panel represents a higher magnification of the portal field admitted by black box. |
PMC1552085_F1_6756.jpg | What is the principal component of this image? | Immunohistochemical detection of AFP in progenitor cells. Immunohistochemical detection of AFP in progenitor cells (oval cells) in the rat liver. The right panel represents a higher magnification of the portal field admitted by black box. |
PMC1552087_F3_6757.jpg | What is the main focus of this visual representation? | Immunohistochemical staining group I (control): Apoptosis are represented by red clusters. The index represented the number of visible apoptotic cancer cells in five fields |
PMC1553433_F2_6758.jpg | What does this image primarily show? | Imaging of subject 006. A) Subject 006 pre-MRI mammogram demonstrating heterogeneously dense breast tissue. There is no evidence of a cancerous lesion. B) Pre-contrast MR image showing an approximately 1.5 cm, smooth, round lesion in the right breast just above the nipple level medial and close to the chest wall (arrowhead). Core biopsy of this lesion demonstrated benign pathology, specifically, fibrosis with focal ductal epithelial hyperplasia [44]. C) Post-contrast MR images showing a small (approximately 1 cm), round, well-delineated enhancing mass (arrow) in the left breast at the 1:00 position. This mass was seen on both the initial delay after contrast injection (left) and the delayed contrast enhanced subtraction images (right). Core biopsy of this lesion indicated infiltrating ductal carcinoma, which was confirmed after removal via modified radical mastectomy [44]. |
PMC1553433_F2_6759.jpg | What is the principal component of this image? | Imaging of subject 006. A) Subject 006 pre-MRI mammogram demonstrating heterogeneously dense breast tissue. There is no evidence of a cancerous lesion. B) Pre-contrast MR image showing an approximately 1.5 cm, smooth, round lesion in the right breast just above the nipple level medial and close to the chest wall (arrowhead). Core biopsy of this lesion demonstrated benign pathology, specifically, fibrosis with focal ductal epithelial hyperplasia [44]. C) Post-contrast MR images showing a small (approximately 1 cm), round, well-delineated enhancing mass (arrow) in the left breast at the 1:00 position. This mass was seen on both the initial delay after contrast injection (left) and the delayed contrast enhanced subtraction images (right). Core biopsy of this lesion indicated infiltrating ductal carcinoma, which was confirmed after removal via modified radical mastectomy [44]. |
PMC1553433_F2_6760.jpg | What is being portrayed in this visual content? | Imaging of subject 006. A) Subject 006 pre-MRI mammogram demonstrating heterogeneously dense breast tissue. There is no evidence of a cancerous lesion. B) Pre-contrast MR image showing an approximately 1.5 cm, smooth, round lesion in the right breast just above the nipple level medial and close to the chest wall (arrowhead). Core biopsy of this lesion demonstrated benign pathology, specifically, fibrosis with focal ductal epithelial hyperplasia [44]. C) Post-contrast MR images showing a small (approximately 1 cm), round, well-delineated enhancing mass (arrow) in the left breast at the 1:00 position. This mass was seen on both the initial delay after contrast injection (left) and the delayed contrast enhanced subtraction images (right). Core biopsy of this lesion indicated infiltrating ductal carcinoma, which was confirmed after removal via modified radical mastectomy [44]. |
PMC1553457_F3_6761.jpg | What can you see in this picture? | Dose distribution (protons) in an axial CT slice through the center of the breast for an early breast cancer patient treated with partial breast irradiation. The isodose contours are represented by different colors (corresponding values are displayed on the upper-right border of the figure). |
PMC1553472_F6_6762.jpg | What key item or scene is captured in this photo? | MR spectroscopy of the auditory cortex. (A) Anatomical transverse MR image of the left auditory cortex with the superimposed borders of the MRS voxel (1.5 × 1.5 × 1.5 cm3). The image shows the anterior borders of the voxel, which extends 1.5 cm in the posterior direction. (B) 1H-MRS spectrum from the auditory cortex of a single subject. NAA denotes N-acetylaspartate; Glx, glutamate/glutamine; Cr, creatine/phosphocreatine; Cho, choline-containing compounds. The original and the fitted spectrum (using the linear combination model, black line) are displayed. |
PMC1553475_F1_6764.jpg | What does this image primarily show? | Patient information included dorsal and forward bending photographs, as well as AP and latero-lateral radiographs. |
PMC1553475_F1_6766.jpg | What is the focal point of this photograph? | Patient information included dorsal and forward bending photographs, as well as AP and latero-lateral radiographs. |
PMC1555578_F1_6768.jpg | What is the focal point of this photograph? | Radiograph of the ankle at presentation: an undisplaced fracture of the medial malleolus is evident. |
PMC1555578_F1_6767.jpg | What is the central feature of this picture? | Radiograph of the ankle at presentation: an undisplaced fracture of the medial malleolus is evident. |
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