image stringlengths 20 66 | question stringclasses 16
values | answer stringlengths 3 10.7k |
|---|---|---|
PMC1569370_F6_7194.jpg | What does this image primarily show? | HCV NS3/4A colocalizes with Cardif at mitochondrial membrane. The localization of HCV proteins and Cardif was studied in Huh7 cells. The cells were transfected with HCV protein expression constructs (NS3/4A, core or NS5A) and 48 h later cells were fixed and stained. The colocalization was visualised by confocal microscopy. Cells were stained for Cardif (endogenous) (A, D, G, J), mitochondria with Mitotracker Red 580 (B), NS3/4A (E), core (H) and NS5A (K) and the signals were merged (C, F, I, L). |
PMC1569372_F1_7196.jpg | What object or scene is depicted here? | Preoperative CT scan of the sinuses. Coronal view demonstrating opacified left maxillary sinus. |
PMC1569858_F1_7203.jpg | What is the dominant medical problem in this image? | Hypoperfusion pattern in Frontotemporal Lobar Degeneration ApoE ε4+ compared to ApoE ε4- carriers: bilateral hippocampal structure involvement. The direct comparison of the perfusion pattern between Apolipoprotein (ApoE) ε4+ vs. ApoE ε4- demonstrated a significant bilateral hypoperfusion in uncus and in parahippocampal gyrus. Talairach and Tournoux coordinates. Coronal slices y = -8 to -13, axial slice z = -24,p < 0.01, T > 2.41, minimum cluster size = 40 voxels. Functional patterns superimposed to standard T1 weighted MRI. |
PMC1569858_F1_7202.jpg | What key item or scene is captured in this photo? | Hypoperfusion pattern in Frontotemporal Lobar Degeneration ApoE ε4+ compared to ApoE ε4- carriers: bilateral hippocampal structure involvement. The direct comparison of the perfusion pattern between Apolipoprotein (ApoE) ε4+ vs. ApoE ε4- demonstrated a significant bilateral hypoperfusion in uncus and in parahippocampal gyrus. Talairach and Tournoux coordinates. Coronal slices y = -8 to -13, axial slice z = -24,p < 0.01, T > 2.41, minimum cluster size = 40 voxels. Functional patterns superimposed to standard T1 weighted MRI. |
PMC1569897_F6A_7206.jpg | Describe the main subject of this image. | Basket mapping of normal sinus rhythm. The right upper panel shows the potential map, the earliest activation (red color) at the site of sinus node. In addition, the splines of basket catheters are aligned with the endocardial wall. The right lower panel shows isochronal map of the sinus rhythm. The left panel shows the electrogram from the different splines. SN= Sinus node; TV= tricuspid valve; AVN= Atrioventricular node. |
PMC1569897_F6A_7204.jpg | What is the core subject represented in this visual? | Basket mapping of normal sinus rhythm. The right upper panel shows the potential map, the earliest activation (red color) at the site of sinus node. In addition, the splines of basket catheters are aligned with the endocardial wall. The right lower panel shows isochronal map of the sinus rhythm. The left panel shows the electrogram from the different splines. SN= Sinus node; TV= tricuspid valve; AVN= Atrioventricular node. |
PMC1569897_F6A_7205.jpg | What is the focal point of this photograph? | Basket mapping of normal sinus rhythm. The right upper panel shows the potential map, the earliest activation (red color) at the site of sinus node. In addition, the splines of basket catheters are aligned with the endocardial wall. The right lower panel shows isochronal map of the sinus rhythm. The left panel shows the electrogram from the different splines. SN= Sinus node; TV= tricuspid valve; AVN= Atrioventricular node. |
PMC1570091_f2-ehp0114-001432_7209.jpg | What is the dominant medical problem in this image? | Eleven-year-old boy with frontal (A) and lateral (B) CXRs that demonstrate hyperinflation. The lateral film shows an increase in the anterior clear space, increased anterior–posterior diameter, and flattening of the hemidiaphragms. |
PMC1570091_f5-ehp0114-001432_7208.jpg | What key item or scene is captured in this photo? | High-resolution axial CT of a 10-year-old boy demonstrating mildly dilated central airways (blue arrows) and mild peribronchial thickening (white arrow). |
PMC1570091_f6-ehp0114-001432_7211.jpg | What is the main focus of this visual representation? | High-resolution expiratory CT of a 9-year-old boy demonstrating air trapping at the level of the secondary pulmonary lobule (arrows). |
PMC1570144_F1_7213.jpg | What is the principal component of this image? | Neurospheres derived from the PTPσ(-/-) knockout mouse brain show normal morphological features. a) Classical appearance of neurospheres in culture. Clonal derived spheres from a PTPσ(-/-) mouse visualized by differential interference contrast microscopy. Scale bar = 50 μm b) Nestin expression in a medium sized neurosphere from a PTPσ(-/-) mouse. This stem cell/progenitor cell marker is uniformly distributed throughout the sphere. Confocal microscopy. Scale bar = 50 μm c) Double labeling for nestin (rhodamine-red) and the EGF-R (fluorescein-green) in a sphere derived from a PTPσ(+/-) mouse. Confocal microscopy. Scale bar = 50 μm d) Immunofluorescent image of the NG2 progenitor cell marker (fluorescein-green) and the neuronal marker tubulin (rhodamine-red) in a neurosphere generated from a PTPσ (-/-) mouse. Epi-fluorescence microscopy. Scale bar = 50 μm. |
PMC1570144_F1_7214.jpg | What is the dominant medical problem in this image? | Neurospheres derived from the PTPσ(-/-) knockout mouse brain show normal morphological features. a) Classical appearance of neurospheres in culture. Clonal derived spheres from a PTPσ(-/-) mouse visualized by differential interference contrast microscopy. Scale bar = 50 μm b) Nestin expression in a medium sized neurosphere from a PTPσ(-/-) mouse. This stem cell/progenitor cell marker is uniformly distributed throughout the sphere. Confocal microscopy. Scale bar = 50 μm c) Double labeling for nestin (rhodamine-red) and the EGF-R (fluorescein-green) in a sphere derived from a PTPσ(+/-) mouse. Confocal microscopy. Scale bar = 50 μm d) Immunofluorescent image of the NG2 progenitor cell marker (fluorescein-green) and the neuronal marker tubulin (rhodamine-red) in a neurosphere generated from a PTPσ (-/-) mouse. Epi-fluorescence microscopy. Scale bar = 50 μm. |
PMC1570144_F1_7215.jpg | What is the focal point of this photograph? | Neurospheres derived from the PTPσ(-/-) knockout mouse brain show normal morphological features. a) Classical appearance of neurospheres in culture. Clonal derived spheres from a PTPσ(-/-) mouse visualized by differential interference contrast microscopy. Scale bar = 50 μm b) Nestin expression in a medium sized neurosphere from a PTPσ(-/-) mouse. This stem cell/progenitor cell marker is uniformly distributed throughout the sphere. Confocal microscopy. Scale bar = 50 μm c) Double labeling for nestin (rhodamine-red) and the EGF-R (fluorescein-green) in a sphere derived from a PTPσ(+/-) mouse. Confocal microscopy. Scale bar = 50 μm d) Immunofluorescent image of the NG2 progenitor cell marker (fluorescein-green) and the neuronal marker tubulin (rhodamine-red) in a neurosphere generated from a PTPσ (-/-) mouse. Epi-fluorescence microscopy. Scale bar = 50 μm. |
PMC1570146_F4_7225.jpg | What object or scene is depicted here? | Ultrastructural examination: (a) Carcinomatous component: glandular epithelial structure with surface microvilla (arrow); in the inset: enlargement of the basement membrane (arrow) and hemidesmosomes (red arrows). (b) Sarcomatous component: spindle cells with actin cytoplasmic thin filaments and focal densities (enlargement in the inset). Scale bar: 2µm. |
PMC1570146_F4_7224.jpg | What is the dominant medical problem in this image? | Ultrastructural examination: (a) Carcinomatous component: glandular epithelial structure with surface microvilla (arrow); in the inset: enlargement of the basement membrane (arrow) and hemidesmosomes (red arrows). (b) Sarcomatous component: spindle cells with actin cytoplasmic thin filaments and focal densities (enlargement in the inset). Scale bar: 2µm. |
PMC1570340_F4_7229.jpg | Describe the main subject of this image. | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570340_F4_7227.jpg | What does this image primarily show? | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570340_F4_7233.jpg | What is being portrayed in this visual content? | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570340_F4_7226.jpg | What key item or scene is captured in this photo? | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570340_F4_7232.jpg | What is the principal component of this image? | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570340_F4_7231.jpg | What key item or scene is captured in this photo? | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570340_F4_7228.jpg | What is the principal component of this image? | Electron micrographs of virus infected BSC40 cells. MOI = 10 and cells were harvested and fixed 24 hours after infection. Dts-4 at the permissive temperature of 31C (A-C). Dts-4 (D), Cts-16 (E), Cts-34 (F), Dts-8 (G), Dts-35 (H) and Dts-93 (I) at the non-permissive temperature of 41C. Bars represent 400 nm except in C, D and F (bar = 200 nm). N, nucleus; m, mitochondria; IMV, intracellular mature virion; asterisk, immature viral particle; arrow, representative particles with asymmetrical viroplasm condensation; arrow head, nucleoids. |
PMC1570343_F1_7235.jpg | What object or scene is depicted here? | CT scan showing right opacified maxillary sinus with medial bulging causing expansion of the sinus and obstruction of the right nasal cavity. |
PMC1570350_F6_7237.jpg | What object or scene is depicted here? | At medium power (×20 magnification), histology from the original breast excision biopsy shows infiltrating strands of tumor cells with some pleomorphism. The tumor also forms circumscribed nodules. |
PMC1570356_F3_7241.jpg | Can you identify the primary element in this image? | Histological analysis of mouse lung sections after theophylline treatment: (a) negative control, (b) untreated (OVA-challenged), (c) unmodified ('pure') chitosan nanoparticles, (d) Thiolated chitosan, (e) theophylline, (f) theophylline plus unmodified chitosan nanoparticles, (g) theophylline plus TCNs. On day 22, lungs were removed, sectioned and stained with hematoxylin/eosin. Examination of tissue sections was performed by different persons in a blinded fashion. The results of a representative experiment of 3 slides for each section are shown. |
PMC1570356_F3_7242.jpg | What object or scene is depicted here? | Histological analysis of mouse lung sections after theophylline treatment: (a) negative control, (b) untreated (OVA-challenged), (c) unmodified ('pure') chitosan nanoparticles, (d) Thiolated chitosan, (e) theophylline, (f) theophylline plus unmodified chitosan nanoparticles, (g) theophylline plus TCNs. On day 22, lungs were removed, sectioned and stained with hematoxylin/eosin. Examination of tissue sections was performed by different persons in a blinded fashion. The results of a representative experiment of 3 slides for each section are shown. |
PMC1570356_F3_7243.jpg | What is the dominant medical problem in this image? | Histological analysis of mouse lung sections after theophylline treatment: (a) negative control, (b) untreated (OVA-challenged), (c) unmodified ('pure') chitosan nanoparticles, (d) Thiolated chitosan, (e) theophylline, (f) theophylline plus unmodified chitosan nanoparticles, (g) theophylline plus TCNs. On day 22, lungs were removed, sectioned and stained with hematoxylin/eosin. Examination of tissue sections was performed by different persons in a blinded fashion. The results of a representative experiment of 3 slides for each section are shown. |
PMC1570356_F3_7238.jpg | What is being portrayed in this visual content? | Histological analysis of mouse lung sections after theophylline treatment: (a) negative control, (b) untreated (OVA-challenged), (c) unmodified ('pure') chitosan nanoparticles, (d) Thiolated chitosan, (e) theophylline, (f) theophylline plus unmodified chitosan nanoparticles, (g) theophylline plus TCNs. On day 22, lungs were removed, sectioned and stained with hematoxylin/eosin. Examination of tissue sections was performed by different persons in a blinded fashion. The results of a representative experiment of 3 slides for each section are shown. |
PMC1570356_F3_7239.jpg | What is the central feature of this picture? | Histological analysis of mouse lung sections after theophylline treatment: (a) negative control, (b) untreated (OVA-challenged), (c) unmodified ('pure') chitosan nanoparticles, (d) Thiolated chitosan, (e) theophylline, (f) theophylline plus unmodified chitosan nanoparticles, (g) theophylline plus TCNs. On day 22, lungs were removed, sectioned and stained with hematoxylin/eosin. Examination of tissue sections was performed by different persons in a blinded fashion. The results of a representative experiment of 3 slides for each section are shown. |
PMC1570356_F3_7244.jpg | What is the main focus of this visual representation? | Histological analysis of mouse lung sections after theophylline treatment: (a) negative control, (b) untreated (OVA-challenged), (c) unmodified ('pure') chitosan nanoparticles, (d) Thiolated chitosan, (e) theophylline, (f) theophylline plus unmodified chitosan nanoparticles, (g) theophylline plus TCNs. On day 22, lungs were removed, sectioned and stained with hematoxylin/eosin. Examination of tissue sections was performed by different persons in a blinded fashion. The results of a representative experiment of 3 slides for each section are shown. |
PMC1570367_F2_7245.jpg | What is shown in this image? | Computed tomography (CT) of the oropharynx with contrast medium. The axial projection shows a smooth bounded nodular neoformation in the right-sided tonsil's region of the oropharynx. The other layers of the tomography didn't reveal signs of enlarged cervical lymph nodes. |
PMC1570367_F3_7247.jpg | What is the dominant medical problem in this image? | A biopsy of the tumor in Hematoxilin-Eosin (HE) staining. Spindle cell tumor with cellular polymorphism, mimicking a storiform growth pattern. Mitoses are also frequent supporting a malignant proliferation. HE 100 × (a), 200 × (b). |
PMC1570367_F3_7248.jpg | Describe the main subject of this image. | A biopsy of the tumor in Hematoxilin-Eosin (HE) staining. Spindle cell tumor with cellular polymorphism, mimicking a storiform growth pattern. Mitoses are also frequent supporting a malignant proliferation. HE 100 × (a), 200 × (b). |
PMC1570383_F2_7249.jpg | What is the principal component of this image? | Distribution and cellular localization of
IL-1β immunoreactivity 7 h after CHI. As
indicated by the dashed line in (a), IL-1β expression is not restricted to the site of primary
cortical injury but extends to deeper brain areas including the
corpus callosum, the basal ganglia, and the piriform cortex as
well. Boxed areas are displayed at higher magnification in
(c), (d), and (e). (b) is a high power view
showing that IL-1β-positive cells have the typical ramified
morphology of resident microglia. |
PMC1570450_F1_7251.jpg | What is the central feature of this picture? | a: Computed tomography (CT) of the cervical abscess. b: Extracted foreign body. A grass blade of 2 cm of length. |
PMC1570450_F1_7250.jpg | What is the focal point of this photograph? | a: Computed tomography (CT) of the cervical abscess. b: Extracted foreign body. A grass blade of 2 cm of length. |
PMC1570454_F1_7253.jpg | Can you identify the primary element in this image? | Observation of N2a-Rα cells by phase contrast microscopy. The parental Neuro2a cells (panel "Neuro2a") were transfected with rat AhR in the mammalian expression vector pcDNA4/V5-His and selected with zeocin. The morphology of the resultant transfectant is shown in panel "N2a-Rα ". The cells transfected with the vector without the insert are shown in panel "N2a-Vc". The N2a-Rα cells exhibited spontaneous neurite outgrowth. Note that no morphological differences in N2a-Vc were observed compared with the parental Neuro2a cells. |
PMC1570454_F1_7252.jpg | What is the core subject represented in this visual? | Observation of N2a-Rα cells by phase contrast microscopy. The parental Neuro2a cells (panel "Neuro2a") were transfected with rat AhR in the mammalian expression vector pcDNA4/V5-His and selected with zeocin. The morphology of the resultant transfectant is shown in panel "N2a-Rα ". The cells transfected with the vector without the insert are shown in panel "N2a-Vc". The N2a-Rα cells exhibited spontaneous neurite outgrowth. Note that no morphological differences in N2a-Vc were observed compared with the parental Neuro2a cells. |
PMC1570456_F1_7257.jpg | What's the most prominent thing you notice in this picture? | Chest CT scan showing a 1 cm right upper lobe nodule. |
PMC1570456_F2_7255.jpg | Can you identify the primary element in this image? | Chest CT scan showing a 1.7 cm left lower lobe nodule (left), as well as, a three months follow up Chest CT scan demonstrating enlargement of the left lower lobe nodule from 1.7 cm to 3 cm (right). |
PMC1570456_F2_7256.jpg | What is the principal component of this image? | Chest CT scan showing a 1.7 cm left lower lobe nodule (left), as well as, a three months follow up Chest CT scan demonstrating enlargement of the left lower lobe nodule from 1.7 cm to 3 cm (right). |
PMC1570460_F2_7258.jpg | What is the core subject represented in this visual? | Immunohistochemical staining for neurone-specific enolase (NSE) of a large-cell carcinoma with neuroendocrine morphology (A) and a large-cell neuroendocrine carcinoma (B). (Magnification: 100×, Stain: NSE). |
PMC1570462_F2_7264.jpg | What key item or scene is captured in this photo? | Colocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugate |
PMC1570462_F2_7263.jpg | Describe the main subject of this image. | Colocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugate |
PMC1570462_F2_7265.jpg | What is being portrayed in this visual content? | Colocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugate |
PMC1570462_F2_7261.jpg | What is the focal point of this photograph? | Colocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugate |
PMC1570462_F2_7262.jpg | What is the dominant medical problem in this image? | Colocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugate |
PMC1570462_F2_7266.jpg | What is shown in this image? | Colocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugate |
PMC1570478_F3_7268.jpg | What stands out most in this visual? | Microscopic features of ovarian fibrothecoma include round and spindle shaped nuclei without atypia or myxoid change. No mitotic figures were observed. Standard H&E (H) and vimentin stain (V), 40× magnification. |
PMC1570478_F3_7267.jpg | What is shown in this image? | Microscopic features of ovarian fibrothecoma include round and spindle shaped nuclei without atypia or myxoid change. No mitotic figures were observed. Standard H&E (H) and vimentin stain (V), 40× magnification. |
PMC1570500_pbio-0040330-g005_7274.jpg | Describe the main subject of this image. | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1570500_pbio-0040330-g005_7273.jpg | What key item or scene is captured in this photo? | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1570500_pbio-0040330-g005_7269.jpg | What is the main focus of this visual representation? | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1570500_pbio-0040330-g005_7272.jpg | Describe the main subject of this image. | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1570500_pbio-0040330-g005_7275.jpg | What stands out most in this visual? | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1570500_pbio-0040330-g005_7280.jpg | Describe the main subject of this image. | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1570500_pbio-0040330-g005_7279.jpg | What's the most prominent thing you notice in this picture? | SNAP-25 Is Essential for Synaptobrevin 2–Triggered Munc18–1 ReleaseMembrane sheets were prepared from mouse embryonal chromaffin cells (E16–E18), isolated from wild-type or snap25 knockout animals. Membrane sheets were incubated for 10 min in the absence or presence of 10 μM synaptobrevin 2, fixed, and immunostained for Munc18–1. Syntaxin was also visualized by immunostaining in order to make sure that membrane sheets were generated from chromaffin cells and not from fibroblast cells, which are also present in the culture (for details see Materials and Methods).(A) and (B) Membrane sheets generated from cells isolated from wild-type (WT) (A) or knockout (B) animals. Left panels, syntaxin staining; right panels, Munc18–1 staining. Upper and lower panels in (A) and (B), absence or presence of synaptobrevin during incubation, respectively.(C) Quantification of Munc18–1 immunostaining intensities. For clarity, values are expressed as percentage relating to values obtained in the absence of synaptobrevin. For each condition, seven independent experiments were performed. Values are given as mean ± SEM. Statistical test. n = 7, paired t-test knockout:wt p < 0.005. |
PMC1574292_F1_7281.jpg | What is the focal point of this photograph? | Structural and ultrastructural (electron microscopy) analyses of human brain tissue sections obtained from the ependymoma (case 9945) and meningitis cases. Aa. H&E staining of paraffin embedded wax tissue sections; original magnification, ×200. The presence of perivascular rosette (Ro) formation is typical of an ependymoma. Ab. Electron micrographs taken of the araldite-enhanced ependymoma cells. Original magnification, ×13500; inset: original magnification, × 23000. The white arrows indicate junction complexes between cells and the black arrows indicate microvilli (see inset). These structures reveal key characteristics of ependymal cells. Ba, Bb, Meningitis cases. Original magnification, ×100. Choroid plexus (a) and ependyma (Ep) (b) show a continuous layer of intact epithelial cells despite the presence of neutrophils (PMN) inside the ventricle (particularly in panel Bb, see inset, magnification ×1000). V, vessel; Cp, choroid plexus; Ep, ependymal layer. |
PMC1574292_F1_7283.jpg | What key item or scene is captured in this photo? | Structural and ultrastructural (electron microscopy) analyses of human brain tissue sections obtained from the ependymoma (case 9945) and meningitis cases. Aa. H&E staining of paraffin embedded wax tissue sections; original magnification, ×200. The presence of perivascular rosette (Ro) formation is typical of an ependymoma. Ab. Electron micrographs taken of the araldite-enhanced ependymoma cells. Original magnification, ×13500; inset: original magnification, × 23000. The white arrows indicate junction complexes between cells and the black arrows indicate microvilli (see inset). These structures reveal key characteristics of ependymal cells. Ba, Bb, Meningitis cases. Original magnification, ×100. Choroid plexus (a) and ependyma (Ep) (b) show a continuous layer of intact epithelial cells despite the presence of neutrophils (PMN) inside the ventricle (particularly in panel Bb, see inset, magnification ×1000). V, vessel; Cp, choroid plexus; Ep, ependymal layer. |
PMC1574292_F1_7282.jpg | What can you see in this picture? | Structural and ultrastructural (electron microscopy) analyses of human brain tissue sections obtained from the ependymoma (case 9945) and meningitis cases. Aa. H&E staining of paraffin embedded wax tissue sections; original magnification, ×200. The presence of perivascular rosette (Ro) formation is typical of an ependymoma. Ab. Electron micrographs taken of the araldite-enhanced ependymoma cells. Original magnification, ×13500; inset: original magnification, × 23000. The white arrows indicate junction complexes between cells and the black arrows indicate microvilli (see inset). These structures reveal key characteristics of ependymal cells. Ba, Bb, Meningitis cases. Original magnification, ×100. Choroid plexus (a) and ependyma (Ep) (b) show a continuous layer of intact epithelial cells despite the presence of neutrophils (PMN) inside the ventricle (particularly in panel Bb, see inset, magnification ×1000). V, vessel; Cp, choroid plexus; Ep, ependymal layer. |
PMC1574292_F1_7284.jpg | What is the main focus of this visual representation? | Structural and ultrastructural (electron microscopy) analyses of human brain tissue sections obtained from the ependymoma (case 9945) and meningitis cases. Aa. H&E staining of paraffin embedded wax tissue sections; original magnification, ×200. The presence of perivascular rosette (Ro) formation is typical of an ependymoma. Ab. Electron micrographs taken of the araldite-enhanced ependymoma cells. Original magnification, ×13500; inset: original magnification, × 23000. The white arrows indicate junction complexes between cells and the black arrows indicate microvilli (see inset). These structures reveal key characteristics of ependymal cells. Ba, Bb, Meningitis cases. Original magnification, ×100. Choroid plexus (a) and ependyma (Ep) (b) show a continuous layer of intact epithelial cells despite the presence of neutrophils (PMN) inside the ventricle (particularly in panel Bb, see inset, magnification ×1000). V, vessel; Cp, choroid plexus; Ep, ependymal layer. |
PMC1574320_F2_7285.jpg | What is the dominant medical problem in this image? | Case 2 ; 2a. Computed tomographic scan of abdomen showing pseudomyxoma peritonei with multiple peritoneal masses (arrow) with "scalloping effect" seen. 2b. Prominent papillary architectures (arrow) (haematoxylin-eosin, × 10); 2c. Papillary architectures with mild to moderate nuclear atypia (arrow) (hematoxylin-eosin, × 20) with no invasion-representing a case of the DPAM variant of PMP. |
PMC1574320_F2_7286.jpg | What object or scene is depicted here? | Case 2 ; 2a. Computed tomographic scan of abdomen showing pseudomyxoma peritonei with multiple peritoneal masses (arrow) with "scalloping effect" seen. 2b. Prominent papillary architectures (arrow) (haematoxylin-eosin, × 10); 2c. Papillary architectures with mild to moderate nuclear atypia (arrow) (hematoxylin-eosin, × 20) with no invasion-representing a case of the DPAM variant of PMP. |
PMC1574330_F2_7289.jpg | What is being portrayed in this visual content? | Liver biopsy, showing large hyphae surrounded by strongly eosinophilic material and an inflammatory cell infiltrate containing histiocytes, multinucleated giant cells and numerous eosinophils. (a) H&E stained (b) Grocott stained sections. |
PMC1574333_F1_7291.jpg | What is the central feature of this picture? | Preoperative CT-angiography shows a complete caval obstruction (arrow) due to a tumoral mass of the right adrenal gland (A); postoperative histology revealed a primary carcinoma with kidney infiltration (B). |
PMC1574333_F2_7290.jpg | Describe the main subject of this image. | Preliminary cavography: caval involvement (arrow) from a retroperitoneal mass (pelvic leiomyomatosis). |
PMC1574333_F3_7293.jpg | What object or scene is depicted here? | Postoperative findings: tumoral thrombus after complete surgical removal (A). Dacron patch angioplasty was used to repair IVC resection to achieve complete tumour excision (B) |
PMC1574333_F3_7295.jpg | What is the main focus of this visual representation? | Postoperative findings: tumoral thrombus after complete surgical removal (A). Dacron patch angioplasty was used to repair IVC resection to achieve complete tumour excision (B) |
PMC1574343_F2_7296.jpg | What is the principal component of this image? | Immuno-precipitation to determine that the lower MW protein in Figure 1 was GIRK1. Two samples of MDA-MB-453 cells were immuno-precipitated with goat polyclonal antibody (Santa Cruz) for GIRK1, then separated by Western blot, and probed with rabbit polyclonal antibody used for Figure 1 (Upstate). The highest expression of GIRK1 was at the lower molecular weight in these immuno-precipitated cells. This is a representative gel of two separate experiments. |
PMC1578562_F9_7305.jpg | What is shown in this image? | Radiograph taken with the faxitron machine: The technique, where the entire bone or bone samples already cut in blocks are radiographed with the faxitron machine, allows the assessment of bony changes, such as bone resorption (arrow head) or bone sclerosis (arrow) as a means for biocompatibility of the inserted biomaterials. In Fig. 9a an entire femur is shown (arrow: Bone-fixation device), whereas in Fig.9b only the original defect with a rim of adjacent bone is pictured. |
PMC1578562_F9_7304.jpg | What can you see in this picture? | Radiograph taken with the faxitron machine: The technique, where the entire bone or bone samples already cut in blocks are radiographed with the faxitron machine, allows the assessment of bony changes, such as bone resorption (arrow head) or bone sclerosis (arrow) as a means for biocompatibility of the inserted biomaterials. In Fig. 9a an entire femur is shown (arrow: Bone-fixation device), whereas in Fig.9b only the original defect with a rim of adjacent bone is pictured. |
PMC1578562_F10_7302.jpg | What key item or scene is captured in this photo? | Microradiographs taken from two different biomaterial samples: Microradiographs show the stage of calcification of the bone after the application of bone enhancing materials (Fig. 10a) or both, remnants of bone cements based on calcium phosphate and replacement by new bone (Fig. 10b). |
PMC1578562_F10_7303.jpg | What's the most prominent thing you notice in this picture? | Microradiographs taken from two different biomaterial samples: Microradiographs show the stage of calcification of the bone after the application of bone enhancing materials (Fig. 10a) or both, remnants of bone cements based on calcium phosphate and replacement by new bone (Fig. 10b). |
PMC1578562_F11_7300.jpg | What is the core subject represented in this visual? | Histology samples: While ground sections (30–40 μm, surface stained with toluidine blue) are well suited for the assessment of osseointegration, new bone formation, materials resorption and histomorphometrical measurement (Fig. 11a), thin sections allow assessing cellular reactions such as degradation and elimination of biomaterials through macrophages (Fig. 11b, arrow) or the appearance of foreign body cells (Fig. 11c, arrow). |
PMC1578562_F11_7299.jpg | What is the principal component of this image? | Histology samples: While ground sections (30–40 μm, surface stained with toluidine blue) are well suited for the assessment of osseointegration, new bone formation, materials resorption and histomorphometrical measurement (Fig. 11a), thin sections allow assessing cellular reactions such as degradation and elimination of biomaterials through macrophages (Fig. 11b, arrow) or the appearance of foreign body cells (Fig. 11c, arrow). |
PMC1578562_F11_7301.jpg | Can you identify the primary element in this image? | Histology samples: While ground sections (30–40 μm, surface stained with toluidine blue) are well suited for the assessment of osseointegration, new bone formation, materials resorption and histomorphometrical measurement (Fig. 11a), thin sections allow assessing cellular reactions such as degradation and elimination of biomaterials through macrophages (Fig. 11b, arrow) or the appearance of foreign body cells (Fig. 11c, arrow). |
PMC1579220_F1_7307.jpg | What is the principal component of this image? | a) Abdominal CT shows the 1.5-cm diameter low-density mass in size in the S3. b) This lesion was slightly enhanced, and its periphery was noticeably enhanced on equilibrium phase. |
PMC1579220_F2_7308.jpg | Can you identify the primary element in this image? | a) MRI showed a low-intensity lesion on the T1-weighted image. b) The border of the lesion was clearly visualized on the T2-weighted image with SPIO. |
PMC1579220_F2_7309.jpg | What's the most prominent thing you notice in this picture? | a) MRI showed a low-intensity lesion on the T1-weighted image. b) The border of the lesion was clearly visualized on the T2-weighted image with SPIO. |
PMC1579220_F5_7310.jpg | What key item or scene is captured in this photo? | Dynamic MRI. a) Plain MRI showed the 1.0-cm diameter low-intensity mass in size in the S2. b) This nodular lesion was enhanced at an early phase. c) At a late phase, this lesion was showed as low-intensity mass than surrounding liver tissue. |
PMC1579220_F5_7312.jpg | What is the principal component of this image? | Dynamic MRI. a) Plain MRI showed the 1.0-cm diameter low-intensity mass in size in the S2. b) This nodular lesion was enhanced at an early phase. c) At a late phase, this lesion was showed as low-intensity mass than surrounding liver tissue. |
PMC1579243_ppat-0020099-g006_7314.jpg | What can you see in this picture? | Virions Showing Two Different Modes of Budding(A) The majority of the virions is emerging horizontally from the cell surface and contains nucleocapsids (B), whereas vertically budding virions appear empty. Insets in (A) and (B) are larger magnifications of the areas identified in boxes of the main pictures, depicting cross sections of VLPs. (C) VLPs induced by the expression of VP40 alone bud vertically and lack NC-like structures. Bars, 400 nm. |
PMC1579243_ppat-0020099-g006_7313.jpg | What key item or scene is captured in this photo? | Virions Showing Two Different Modes of Budding(A) The majority of the virions is emerging horizontally from the cell surface and contains nucleocapsids (B), whereas vertically budding virions appear empty. Insets in (A) and (B) are larger magnifications of the areas identified in boxes of the main pictures, depicting cross sections of VLPs. (C) VLPs induced by the expression of VP40 alone bud vertically and lack NC-like structures. Bars, 400 nm. |
PMC1579245_ppat-0020101-g006_7316.jpg | What is the central feature of this picture? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7325.jpg | Describe the main subject of this image. | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7318.jpg | What stands out most in this visual? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7326.jpg | What key item or scene is captured in this photo? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7320.jpg | What object or scene is depicted here? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7324.jpg | What is the focal point of this photograph? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7323.jpg | What is the principal component of this image? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7322.jpg | What does this image primarily show? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7327.jpg | Can you identify the primary element in this image? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g006_7317.jpg | What's the most prominent thing you notice in this picture? | FAZ Replication Is Not Sufficient to Drive Cytokinesis in Bloodstream Form ParasitesImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-FAZ monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7339.jpg | What's the most prominent thing you notice in this picture? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7330.jpg | Can you identify the primary element in this image? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7336.jpg | What object or scene is depicted here? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7329.jpg | What can you see in this picture? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7333.jpg | What is the focal point of this photograph? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7332.jpg | What is the core subject represented in this visual? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
PMC1579245_ppat-0020101-g007_7331.jpg | What object or scene is depicted here? | BSF-TPN Mutants Proceed through Multiple Rounds of Flagellum Replication, but Ultimately Fail to Initiate CytokinesisImmunofluorescence analysis of BSF-TPN-B cells grown in the absence (A) or presence (B–F) of tetracycline for 24 h. Cytoskeletons were prepared by detergent extraction and used for immunofluorescence with α-PFR-2 (A–F) monoclonal antibodies. Phase-contrast images are shown in the left panels and merged images are shown in the right panels, with antibody staining in red and DAPI staining in blue. Arrowheads indicate examples of cells with more than two flagella and a single diffuse nucleus. Arrows indicate examples of cells with more than two flagella and two or more nuclei. Scalebar is 10 μm. |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.