Search is not available for this dataset
article
stringlengths
4.36k
149k
summary
stringlengths
32
3.35k
section_headings
listlengths
1
91
keywords
listlengths
0
141
year
stringclasses
13 values
title
stringlengths
20
281
Lymphedema management programs have been shown to decrease episodes of adenolymphangitis ( ADLA ) , but the impact on lymphedema progression and of program compliance have not been thoroughly explored . Our objectives were to determine the rate of ADLA episodes and lymphedema progression over time for patients enrolled...
Lymphatic filariasis ( LF ) is characterized by clinical manifestations of limb swelling , lymphedema , and elephantiasis . LF is the world's second-leading cause of disability , with up to 15 million people with limb lymphedema or elephantiasis . The Global Programme to Eliminate LF aims to eliminate the disease throu...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "helminth", "infections", "infectious", "diseases", "medicine", "and", "health", "sciences", "filariasis", "neglected", "tropical", "diseases", "lymphatic", "filariasis", "tropical", "diseases", "parasitic", "diseases" ]
2014
Impact of a Community-Based Lymphedema Management Program on Episodes of Adenolymphangitis (ADLA) and Lymphedema Progression - Odisha State, India
The island of Martha's Vineyard , Massachusetts , is the site of a sustained outbreak of tularemia due to Francisella tularensis tularensis . Dog ticks , Dermacentor variabilis , appear to be critical in the perpetuation of the agent there . Tularemia has long been characterized as an agent of natural focality , stably...
We present evidence , for the first time , that the agent of tularemia persists in microfoci for at least four years . The existing literature alludes to the natural nidality of this bacterium and the importance thereof in its long-term survival in nature , but “natural foci” that have been described to date have been ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "public", "health", "and", "epidemiology/infectious", "diseases", "ecology/environmental", "microbiology" ]
2009
Nonrandom Distribution of Vector Ticks (Dermacentor variabilis) Infected by Francisella tularensis
Currently , knowledge does not allow early prediction of which cases of dengue fever ( DF ) will progress to dengue hemorrhagic fever ( DHF ) , to allow early intervention to prevent progression or to limit severity . The objective of this study is to investigate the hypothesis that some specific comorbidities increase...
Dengue is a virus disease that has already reached more than 100 countries worldwide , transmitted by Aedes mosquitos , mainly Aedes aegypti . It is estimated that annually nearly 96 million symptomatic cases and about 22 , 000 deaths occur . This virus most often manifests itself in the form of Dengue Fever ( DF ) , w...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Arterial Hypertension and Skin Allergy Are Risk Factors for Progression from Dengue to Dengue Hemorrhagic Fever: A Case Control Study
It has been recently shown that the coarse-graining of the structures of polypeptide chains as self-avoiding tubes can provide an effective representation of the conformational space of proteins . In order to fully exploit the opportunities offered by such a ‘tube model’ approach , we present here a strategy to combine...
Modelling protein behaviour using computer simulations has progressively emerged in the last 50 years as a powerful strategy in structural and molecular biology . Over this period there has been a continuing interest in pushing the boundaries of this approach in terms of the size of the systems and the timescale of the...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[]
2015
Mapping the Protein Fold Universe Using the CamTube Force Field in Molecular Dynamics Simulations
Well-studied innate immune systems exist throughout bacteria and archaea , but a more recently discovered genomic locus may offer prokaryotes surprising immunological adaptability . Mediated by a cassette-like genomic locus termed Clustered Regularly Interspaced Short Palindromic Repeats ( CRISPR ) , the microbial adap...
Most microbes appear unculturable in the laboratory , limiting our knowledge of how virus and prokaryotic host evolve in natural systems . However , a genomic locus found in many prokaryotes , CRISPR , may offer cultivation-independent probes of virus-microbe coevolution . Utilizing nearby genes , CRISPR can serially i...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genomics", "environmental", "sciences", "theoretical", "biology", "ecology", "earth", "sciences", "immunology", "biology", "computational", "biology", "evolutionary", "biology", "microbiology", "population", "biology", "genetics", "and", "genomics" ]
2012
Persisting Viral Sequences Shape Microbial CRISPR-based Immunity
The spatial patterning of proteins in bacteria plays an important role in many processes , from cell division to chemotaxis . In the asymmetrically dividing bacteria Caulobacter crescentus , a scaffolding protein , PopZ , localizes to both poles and aids the differential patterning of proteins between mother and daught...
A key process in biology is the self-assembly of biomolecules into highly organized structures . This spontaneous assembly can give rise to complex spatial patterns that help give spatial order to the cellular environment . In many bacteria , the patterning of proteins to the cell poles allows the bacteria to different...
[ "Abstract", "Introduction", "Results", "Discussion", "Model" ]
[ "cell", "biology", "biophysics/theory", "and", "simulation", "computational", "biology" ]
2010
Chromosome Driven Spatial Patterning of Proteins in Bacteria
Intracellular traffic in Aspergillus nidulans hyphae must cope with the challenges that the high rates of apical extension ( 1μm/min ) and the long intracellular distances ( >100 μm ) impose . Understanding the ways in which the hyphal tip cell coordinates traffic to meet these challenges is of basic importance , but i...
Filamentous fungi form long tubular cells , called hyphae , which grow rapidly by apical extension , enabling these sessile organisms to explore substrates and facilitating tissue invasion in the case of pathogenic species . Because the shape of the hyphae is determined by an external cell wall , hyphal growth requires...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "chitin", "cell", "physiology", "vesicles", "aspergillus", "cell", "processes", "fungal", "structure", "cell", "polarity", "aspergillus", "nidulans", "fungi", "dyneins", "molecular", "motors", "materials", "science", "experimental", "organism", "systems", "cellular", "s...
2018
Endocytic recycling via the TGN underlies the polarized hyphal mode of life
The tumor necrosis factor ( TNF ) -receptor 1–associated death domain protein ( TRADD ) mediates induction of apoptosis as well as activation of NF-κB by cellular TNF-receptor 1 ( TNFR1 ) . TRADD is also recruited by the latent membrane protein 1 ( LMP1 ) oncoprotein of Epstein-Barr virus , but its role in LMP1 signali...
For viral infection to succeed , viral proteins must interact with the cellular signaling machinery of its target cell . An oncoprotein encoded by the Epstein-Barr virus ( EBV ) called latent membrane protein 1 ( LMP1 ) is a primary contributor to the transformation of human B cells by the virus and the development of ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "cell", "biology", "virology", "genetics", "and", "genomics" ]
2008
The Viral Oncoprotein LMP1 Exploits TRADD for Signaling by Masking Its Apoptotic Activity
Heterotrimeric G-protein signaling pathways are vital components of physiology , and many are amenable to pharmacologic manipulation . Here , we identify functional heterotrimeric G-protein subunits in Entamoeba histolytica , the causative agent of amoebic colitis . The E . histolytica Gα subunit EhGα1 exhibits convent...
Entamoeba histolytica causes an estimated 50 million intestinal infections and 100 , 000 deaths per year worldwide . Here , we identify functional heterotrimeric G-protein subunits in Entamoeba histolytica , constituting a signaling pathway which , when perturbed , is seen to regulate multiple cellular processes requir...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "proteins", "protein", "structure", "genetics", "biology", "microbiology", "genetics", "and", "genomics", "parasitology" ]
2012
Heterotrimeric G-protein Signaling Is Critical to Pathogenic Processes in Entamoeba histolytica
The mammalian circadian clock and the cell cycle are two major biological oscillators whose coupling influences cell fate decisions . In the present study , we use a model-driven experimental approach to investigate the interplay between clock and cell cycle components and the dysregulatory effects of RAS on this coupl...
In mammals , the circadian clock controls the punctual regulation of biological processes , which , in turn , affect physiology and behaviour , allowing for the synchronisation of internal time to environmental light-dark cycles . Malfunctions of the circadian clock are associated with pathological phenotypes including...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "cycle", "and", "cell", "division", "cell", "processes", "biological", "cultures", "mathematical", "models", "circadian", "oscillators", "chronobiology", "molecular", "biology", "techniques", "research", "and", "analysis", "methods", "sw480", "cells", "mathemati...
2017
The Ink4a/Arf locus operates as a regulator of the circadian clock modulating RAS activity
The molecular mechanisms through which dendritic cells ( DCs ) prime T helper 2 ( Th2 ) responses , including those elicited by parasitic helminths , remain incompletely understood . Here , we report that soluble egg antigen ( SEA ) from Schistosoma mansoni , which is well known to drive potent Th2 responses , triggers...
T helper 2 ( Th2 ) responses , which are initiated by dendritic cells ( DCs ) , can cause allergic diseases , but they can also provide protection against metabolic disorders and parasitic helminth infections . As such , there is great interest in better understanding how their activity is induced and regulated by DCs ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "flow", "cytometry", "schistosoma", "invertebrates", "schistosoma", "mansoni", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "cytokines", "chemical", "compounds", "helminths", "immunol...
2018
Dectin-1/2–induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
Abilities of bacterial pathogens to adapt to the iron limitation present in hosts is critical to their virulence . Bacterial pathogens have evolved diverse strategies to coordinately regulate iron metabolism and virulence associated functions to maintain iron homeostasis in response to changing iron availability in the...
Pathogenic bacteria exhibit tight regulation of iron homeostasis in order to meet iron requirements of living in different environmental conditions , including in the host . The ferric uptake regulator ( Fur ) regulates the expression of genes involved in iron metabolism in response to change in iron availability in se...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "motility", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "regulator", "genes", "plant", "science", "gene", "types", "plant", "pathology", "bacteria", "gene", "expression", "plant", ...
2016
Co-regulation of Iron Metabolism and Virulence Associated Functions by Iron and XibR, a Novel Iron Binding Transcription Factor, in the Plant Pathogen Xanthomonas
Gene expression regulators , such as transcription factors ( TFs ) and microRNAs ( miRNAs ) , have varying regulatory targets based on the tissue and physiological state ( context ) within which they are expressed . While the emergence of regulator-characterizing experiments has inferred the target genes of many regula...
Gene expression regulators , such as transcription factors and microRNAs , are critical actors in cellular physiology and pathophysiology and act by modulating the expression levels of sets of target genes . Given their significance , numerous experiments have sought to characterize the specific target genes of specifi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "breast", "tumors", "clinical", "research", "design", "gene", "regulation", "cancers", "and", "neoplasms", "oncology", "research", "design", "genomic", "databases", "regulator", "genes", "micrornas", "mathematics", "statistics", ...
2017
Contextual Refinement of Regulatory Targets Reveals Effects on Breast Cancer Prognosis of the Regulome
The adult human intestine contains trillions of bacteria , representing hundreds of species and thousands of subspecies . Little is known about the selective pressures that have shaped and are shaping this community's component species , which are dominated by members of the Bacteroidetes and Firmicutes divisions . To ...
The total number of microbes that colonize the surfaces of our adult bodies is thought to be ten times greater than the total number of our human cells . Our microbial partners provide us with certain features that we have not had to evolve on our own . In this sense , we should consider ourselves to be a supraorganism...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "ecology", "gastroenterology", "and", "hepatology", "microbiology", "computational", "biology", "evolutionary", "biology", "homo", "(human)", "genetics", "and", "genomics", "eubacteria" ]
2007
Evolution of Symbiotic Bacteria in the Distal Human Intestine
The Arabidopsis protein DELAY OF GERMINATION 1 ( DOG1 ) is a key regulator of seed dormancy , which is a life history trait that determines the timing of seedling emergence . The amount of DOG1 protein in freshly harvested seeds determines their dormancy level . DOG1 has been identified as a major dormancy QTL and vari...
The Arabidopsis protein DELAY OF GERMINATION 1 ( DOG1 ) is an important regulator of seed dormancy and controls the timing of seedling emergence . The amount of DOG1 protein in mature seeds correlates with their dormancy level . It has been demonstrated that DOG1 is an important contributor to natural variation for see...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Seed Dormancy in Arabidopsis Requires Self-Binding Ability of DOG1 Protein and the Presence of Multiple Isoforms Generated by Alternative Splicing
Naja annulifera is a medically important venomous snake occurring in some of the countries in Sub-Saharan Africa . Accidental bites result in severe coagulation disturbances , systemic inflammation and heart damage , as reported in dogs , and death , by respiratory arrest , in humans . Despite the medical importance of...
N . annulifera is a dangerous snake that belongs to the Elapidae family . It is found in some of the countries in Sub-Saharan Africa and has caused accidents in humans and dogs . In this study , we characterized some of the biochemical , toxic and immunogenic properties of N . annulifera venom . We showed that the veno...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "toxins", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "enzymes", "immunology", "enzymology", "vertebrates", "animals", "mammals", "toxicology", "toxic", "agents", "dogs", "serine", "proteases", "animal",...
2019
Naja annulifera Snake: New insights into the venom components and pathogenesis of envenomation
Various approaches can be applied to uncover the genetic basis of natural phenotypic variation , each with their specific strengths and limitations . Here , we use a replicated genome-wide association approach ( Pool-GWAS ) to fine-scale map genomic regions contributing to natural variation in female abdominal pigmenta...
Phenotypic variation is abundant in natural populations , but its genetic basis is not always well-understood . Here , we examine the genetic basis of body pigmentation in Drosophila , a trait with a long history of study in Drosophila genetics and evolution . We conducted the first genome-wide scan for polymorphism as...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "genome-wide", "association", "studies", "genetics", "population", "genetics", "biology", "evolutionary", "biology", "evolutionary", "genetics" ]
2013
A Genome-Wide, Fine-Scale Map of Natural Pigmentation Variation in Drosophila melanogaster
The majority of Mycobacterium tuberculosis ( Mtb ) infections are clinically latent , characterized by drug tolerance and little or no bacterial replication . Low oxygen tension is a major host factor inducing bacteriostasis , but the molecular mechanisms driving oxygen-dependent replication are poorly understood . Her...
Exposure to Mycobacterium tuberculosis ( Mtb ) can result in a latent form of tuberculosis ( TB ) infection , which can then reactivate and progress to active disease . With 1 . 8 billion infected persons and no tools to predict who will proceed to active disease , latency and reactivation are among the major challenge...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacterial", "pathogens", "biology", "microbiology" ]
2014
Mycobacterium tuberculosis Ser/Thr Protein Kinase B Mediates an Oxygen-Dependent Replication Switch
Chromatin remodeling complexes are essential for gene expression programs that coordinate cell function with metabolic status . However , how these remodelers are integrated in metabolic stability pathways is not well known . Here , we report an expansive genetic screen with chromatin remodelers and metabolic regulator...
Cells coordinate their metabolism with the nutrient environment in order to adapt and thrive . One of the key ways that cells regulate their metabolism is through changes in metabolic gene expression . The transcription of genes is often regulated by manipulating chromatin , which is the packaging material of eukaryoti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "chemical", "compounds", "gene", "regulation", "dna-binding", "proteins", "organic", "compounds", "regulator", "genes", "gene", "types", "epigenetics", "genetic", "interactions", "alcohols", "chromatin", "chromosome", "biology", "proteins", "gene", "expression", "chemistr...
2018
The INO80 chromatin remodeler sustains metabolic stability by promoting TOR signaling and regulating histone acetylation
A central mechanism of virulence of extracellular bacterial pathogens is the injection into host cells of effector proteins that modify host cellular functions . HopW1 is an effector injected by the type III secretion system that increases the growth of the plant pathogen Pseudomonas syringae on the Columbia accession ...
Eukaryotic cells require a dynamic actin cytoskeleton for basic functions , some of which are important for immune responses . Such functions include the transport of cellular material to and from different cellular compartments . The plant pathogen Pseudomonas syringae is extracellular and causes disease by injecting ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "pathogens", "plant", "pathology", "biology", "and", "life", "sciences", "plant", "science" ]
2014
HopW1 from Pseudomonas syringae Disrupts the Actin Cytoskeleton to Promote Virulence in Arabidopsis
Antimicrobial resistance in Staphylococcus aureus is a major public health threat , compounded by emergence of strains with resistance to vancomycin and daptomycin , both last line antimicrobials . Here we have performed high throughput DNA sequencing and comparative genomics for five clinical pairs of vancomycin-susce...
The treatment of serious infections caused by Staphylococcus aureus is complicated by the development of antibiotic resistance , and recently resistance to one of the last available antibiotics to treat resistant S . aureus infections , vancomycin , has also emerged . Here we have shown using whole genome sequencing of...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "genome", "expression", "analysis", "microbial", "mutation", "functional", "genomics", "microbiology", "staphylococcus", "aureus", "genome", "sequencing", "bacterial", "diseases", "methicillin-resistant", "staphylococcus", "aureus", "staphylococci", "bacterial", "...
2011
Evolution of Multidrug Resistance during Staphylococcus aureus Infection Involves Mutation of the Essential Two Component Regulator WalKR
The significance of introgression as an evolutionary force shaping natural populations is well established , especially in animal and plant systems . However , the abundance and size of introgression tracts , and to what degree interspecific gene flow is the result of adaptive processes , are largely unknown . In this ...
Introgression is a process by which genetic material from one species becomes infiltrated into another , genetically distinct species . Introgression usually occurs via sexual reproduction: individuals of two species mate and produce a hybrid offspring , then the offspring repeatedly backcross with one of the parental ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "model", "organisms", "genetics", "biology", "genomics", "evolutionary", "biology", "microbiology", "genetics", "and", "genomics" ]
2012
Large-Scale Introgression Shapes the Evolution of the Mating-Type Chromosomes of the Filamentous Ascomycete Neurospora tetrasperma
Symptoms of schizophrenia may arise from a failure of cortical circuits to filter-out irrelevant inputs . Schizophrenia has also been linked to disruptions in cortical inhibitory interneurons , consistent with the possibility that in the normally functioning brain , these cells are in some part responsible for determin...
Individuals with schizophrenia have difficulty ignoring ideas and experiences that most people would treat as unimportant . There is evidence that this may be due to changes in neuronal inhibition , suggesting that inhibitory neurons may be involved in learning to ignore irrelevant inputs . By developing a computationa...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "learning", "medicine", "and", "health", "sciences", "neural", "networks", "nervous", "system", "brain", "social", "sciences", "electrophysiology", "neuroscience", "learning", "and", "memory", "simulation", "and", "modeling", "cognitive", "psychology", "neuronal", "plas...
2018
Irrelevance by inhibition: Learning, computation, and implications for schizophrenia
The Myc family of transcription factors regulates a variety of biological processes , including the cell cycle , growth , proliferation , metabolism , and apoptosis . In Caenorhabditis elegans , the “Myc interaction network” consists of two opposing heterodimeric complexes with antagonistic functions in transcriptional...
Transcription factors are essential proteins that regulate the expression of genes and play an important role in most biological processes . The results of our study presented here demonstrate for the first time a role in aging for a small family of transcription factors in the nematode worm Caenorhabditis elegans . Im...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "aging", "biochemistry", "developmental", "biology", "physiological", "processes", "medicine", "and", "health", "sciences", "organism", "development", "cell", "biology", "physiology", "gene", "expression", "genetics", "biology", "and", "life", "sciences", "molecular", "...
2014
The Caenorhabditis elegans Myc-Mondo/Mad Complexes Integrate Diverse Longevity Signals
Macroparasite infections ( e . g . , helminths ) remain a major human health concern . However , assessing transmission dynamics is problematic because the direct observation of macroparasite dispersal among hosts is not possible . We used a novel landscape genetics approach to examine transmission of the human roundwo...
Currently , knowledge of transmission patterns of human helminth parasites is based on traditional epidemiological data such as the number of parasites within hosts . Genetic markers can greatly facilitate our understanding of the transmission process because they provide an indirect means to infer dispersal . Here , w...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/helminth", "infections", "infectious", "diseases/epidemiology", "and", "control", "of", "infectious", "diseases" ]
2010
Landscape Genetics Reveals Focal Transmission of a Human Macroparasite
Invertebrates were long thought to possess only a simple , effective and hence non-adaptive defence system against microbial and parasitic attacks . However , recent studies have shown that invertebrate immunity also relies on immune receptors that diversify ( e . g . in echinoderms , insects and mollusks ( Biomphalari...
Contrary to the traditional view that immunity in invertebrates is limited to non-specific mechanisms , recent studies have shown that they have diverse , specific immune receptors . An example is provided by the FREPs of the mollusk Biomphalaria glabrata , polymorphic members of the immunoglobulin superfamily . This c...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "evolutionary", "biology/microbial", "evolution", "and", "genomics", "genetics", "and", "genomics/gene", "discovery", "infectious", "diseases/neglected", "tropical", "diseases", "molecular", "biology/molecular", "evolution", "molecular", "biology/recombination", "molecular", "b...
2008
Controlled Chaos of Polymorphic Mucins in a Metazoan Parasite (Schistosoma mansoni) Interacting with Its Invertebrate Host (Biomphalaria glabrata)
The host species composition in a household and their relative availability affect the host-feeding choices of blood-sucking insects and parasite transmission risks . We investigated four hypotheses regarding factors that affect blood-feeding rates , proportion of human-fed bugs ( human blood index ) , and daily human-...
The major vectors of Chagas disease are species of triatomine bugs that have adapted to human sleeping quarters and may feed on domestic animals and humans . There is a striking lack of information on the blood-feeding rates of Triatominae in field conditions , and factors modifying the fraction of bugs that feed on hu...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "ecology", "and", "environmental", "sciences", "medicine", "and", "health", "sciences", "ecology", "epidemiology", "biology", "and", "life", "sciences", "population", "biology", "population", "ecology" ]
2014
Domestic Animal Hosts Strongly Influence Human-Feeding Rates of the Chagas Disease Vector Triatoma infestans in Argentina
Enterohemorrhagic Escherichia coli ( EHEC ) colonize intestinal epithelium by generating characteristic attaching and effacing ( AE ) lesions . They are lysogenized by prophage that encode Shiga toxin 2 ( Stx2 ) , which is responsible for severe clinical manifestations . As a lysogen , prophage genes leading to lytic g...
Enterohemorrhagic Escherichia coli ( EHEC ) , a food-borne pathogen that produces Shiga toxin , is associated with serious disease outbreaks worldwide , including over 390 food poisoning outbreaks in the U . S . in the last two decades . Humans acquire EHEC by ingesting contaminated food or water , or through contact w...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "bacteriology", "medicine", "and", "health", "sciences", "toxins", "pathology", "and", "laboratory", "medicine", "bacteriophages", "animal", "models", "of", "disease", "microbiology", "toxicology", "toxic", "agents", "viruses", "animal", "models", "bacterial", "diseases...
2019
Prophage induction, but not production of phage particles, is required for lethal disease in a microbiome-replete murine model of enterohemorrhagic E. coli infection
The rise of transcranial current stimulation ( tCS ) techniques have sparked an increasing interest in the effects of weak extracellular electric fields on neural activity . These fields modulate ongoing neural activity through polarization of the neuronal membrane . While the somatic polarization has been investigated...
Transcranial current stimulations ( tCS ) is a brain stimulation technique consisting in the application of low amplitude electric current on a subject’s scalp . Despite evidence of its ability to modify cognitive capacity , little is known on its exact mechanism of action on neural tissues . In this modelling work , w...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "resonance", "frequency", "medicine", "and", "health", "sciences", "action", "potentials", "membrane", "potential", "electricity", "electrophysiology", "neuroscience", "ganglion", "cells", "electric", "field", "neuronal", "dendrites", "animal", "cells", "resonance", "phys...
2018
Differential polarization of cortical pyramidal neuron dendrites through weak extracellular fields
Chromosome duplication and transmission into daughter cells requires the precisely orchestrated binding and release of cohesin . We found that the Drosophila histone chaperone NAP1 is required for cohesin release and sister chromatid resolution during mitosis . Genome-wide surveys revealed that NAP1 and cohesin co-loca...
Eukaryotic DNA is assembled into a nucleo-protein structure called chromatin . Nucleosomes are the basic building blocks of chromatin , comprising 147 bp of DNA tightly wrapped around a histone protein core . Histone chaperones mediate nucleosome assembly by preventing non-productive aggregation of histones with DNA . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A
The tsetse fly , Glossina morsitans morsitans , is a significant problem in Zambia and Malawi . It is the vector for the human infective parasite Trypanosoma brucei rhodesiense , which causes human African trypanosomiasis , and various Trypanosoma species , which cause African animal trypanosomiasis . Understanding the...
Techniques from population genetics have been widely applied to assess problems in evolutionary biology , computational biology , wildlife conservation , animal breeding , etc . In this study , we used population genetics approaches to elucidate the genetic population structure of the tsetse fly Glossina morsitans mors...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "animal", "types", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "population", "genetics", "geographical", "locations", "malawi", "animals", "parasitic", "protozoans", "genetic", "mapping", "glossina", "protozoans...
2019
Genetic diversity and population structure of Glossina morsitans morsitans in the active foci of human African trypanosomiasis in Zambia and Malawi
Submarine hydrothermal vents are model systems for the Archaean Earth environment , and some sites maintain conditions that may have favored the formation and evolution of cellular life . Vents are typified by rapid fluctuations in temperature and redox potential that impose a strong selective pressure on resident micr...
Extreme environments , such as deep-sea hydrothermal vents , found 2 , 500 meters below the ocean surface , support large macrofaunal communities via microbially mediated carbon fixation processes using chemicals ( chemoautotrophy ) rather than light ( photoautotrophy ) . The genome of one such model chemoautotrophic m...
[ "Abstract", "Introduction", "Results/Discussion", "Material", "and", "Methods" ]
[ "microbiology/environmental", "microbiology", "genetics", "and", "genomics/microbial", "evolution", "and", "genomics", "ecology/evolutionary", "ecology", "genetics", "and", "genomics/comparative", "genomics", "microbiology/microbial", "evolution", "and", "genomics", "ecology/envi...
2009
Adaptations to Submarine Hydrothermal Environments Exemplified by the Genome of Nautilia profundicola
Amoebiasis is a major public health problem in tropical and subtropical countries . Currently , metronidazole is the gold choice medication for the treatment of this disease . However , reports have indicated towards the possibility of development of metronidazole-resistance in Entamoeba strains in near future . In vie...
Intestinal amoebiasis , caused by Enatmoeba histolytica continues to be a major public health problem in tropical and subtropical countries and is considered to be the third principal parasitic disease responsible for mortality in the world . In addition to the mutagenic ability and known toxicity of conventional anti-...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biology" ]
2011
Evaluation of Amoebicidal Potential of Paneth Cell Cryptdin-2 against Entamoeba histolytica
MicroRNAs ( miRNAs ) regulate gene expression and play critical roles in growth and development as well as stress responses in eukaryotes . miRNA biogenesis in plants requires a processing complex that consists of the core components DICER-LIKE 1 ( DCL1 ) , SERRATE ( SE ) and HYPONASTIC LEAVES ( HYL1 ) . Here we show t...
miRNAs play critical roles in various biological processes . miRNA biogenesis in both animals and plants are generated by a miRNA processing complex . Studies on specific miRNA function in different biological process have been extensively reported . However , how miRNA biogenesis is modulated , especially by environme...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "plant", "anatomy", "gene", "regulation", "brassica", "protein", "abundance", "biochemical", "analysis", "micrornas", "model", "organisms", "plant", "science", "enzyme", "assays", "in", "vitro", "kinase", "assay", "experimental", "organism", "systems"...
2017
The SnRK2 kinases modulate miRNA accumulation in Arabidopsis
Circadian rhythms are daily biological oscillations driven by an endogenous mechanism known as circadian clock . The protein kinase CK2 is one of the few clock components that is evolutionary conserved among different taxonomic groups . CK2 regulates the stability and nuclear localization of essential clock proteins in...
Most organisms are able to rhythmically coordinate their physiology and metabolism in consonance with the day-night cycle . The cellular mechanism responsible for generating the biological rhythms is known as circadian clock . In contrast to many other biochemical reactions , the pace of the clock does not change with ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "biology/plant-environment", "interactions", "plant", "biology/plant", "growth", "and", "development", "plant", "biology/plant", "genetics", "and", "gene", "expression", "plant", "biology" ]
2010
The Functional Interplay between Protein Kinase CK2 and CCA1 Transcriptional Activity Is Essential for Clock Temperature Compensation in Arabidopsis
Helminth infections are known to regulate cytokine responses in both pulmonary and latent tuberculosis infection . Whether helminth infections also modulate cytokine responses in extra-pulmonary tuberculosis , specifically tuberculous lymphadenitis ( TBL ) , has not been examined thus far . Hence , to determine the cyt...
Strongyloides stercoralis ( Ss ) infects about 30–100 million people worldwide and it is the main causative agent of strongyloidiasis , a chronic parasitic infection . Similarly , tuberculosis ( TB ) affects nearly 2 billion people and both Ss and TB are co-endemic as well as share a major global disease burden . Earli...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "physiology", "cytokines", "helminths", "immunology", "tropical", "diseases", "parasitic", "diseases", "animals", "bacterial", "diseases", "developmental", "biology", "lymph"...
2019
Helminth mediated modulation of the systemic and mycobacterial antigen – stimulated cytokine profiles in extra-pulmonary tuberculosis
Latency-associated nuclear antigen ( LANA ) mediates γ2-herpesvirus genome persistence and regulates transcription . We describe the crystal structure of the murine gammaherpesvirus-68 LANA C-terminal domain at 2 . 2 Å resolution . The structure reveals an alpha-beta fold that assembles as a dimer , reminiscent of Epst...
Herpesviruses establish life-long latent infections . During latency , gammaherpesviruses , such as Kaposi's sarcoma-associated herpesvirus ( KSHV ) , persist as multicopy , circularized genomes in the cell nucleus and express a small subset of viral genes . KSHV latency-associated nuclear antigen ( LANA ) is the predo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Crystal Structure of the Gamma-2 Herpesvirus LANA DNA Binding Domain Identifies Charged Surface Residues Which Impact Viral Latency
Flocks of starlings exhibit a remarkable ability to maintain cohesion as a group in highly uncertain environments and with limited , noisy information . Recent work demonstrated that individual starlings within large flocks respond to a fixed number of nearest neighbors , but until now it was not understood why this nu...
Starling flocks move in beautiful ways that both captivate and intrigue the observer . Previous work has shown that starlings pay attention to their seven closest neighbors , but until now it was not understood why this number is seven . Our paper explains the mystery: when uncertainty in sensing is present , interacti...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "animal", "behavior", "ecology", "evolutionary", "biology", "theoretical", "ecology", "biology", "behavioral", "ecology" ]
2013
Starling Flock Networks Manage Uncertainty in Consensus at Low Cost
Total cholesterol , low-density lipoprotein cholesterol , triglyceride , and high-density lipoprotein cholesterol ( HDL-C ) levels are among the most important risk factors for coronary artery disease . We tested for gene–gene interactions affecting the level of these four lipids based on prior knowledge of established...
Genome-wide association studies ( GWAS ) have identified many loci associated with complex human traits or diseases . However , the fraction of heritable variation explained by these loci is often relatively low . Gene–gene interactions might play a significant role in complex traits or diseases and are one of the many...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome-wide", "association", "studies", "genetic", "association", "studies", "genetics", "biology", "human", "genetics", "genetics", "and", "genomics" ]
2012
Knowledge-Driven Analysis Identifies a Gene–Gene Interaction Affecting High-Density Lipoprotein Cholesterol Levels in Multi-Ethnic Populations
Non-Hodgkin's lymphoma is a disseminated , highly malignant cancer , with resistance to drug treatment based on molecular- and tissue-scale characteristics that are intricately linked . A critical element of molecular resistance has been traced to the loss of functionality in proteins such as the tumor suppressor p53 ....
Non-Hodgkin's lymphoma is a cancer that develops from white blood cells called lymphocytes in the immune system , whose role is to fight disease throughout the body . This cancer can spread throughout the whole body and be very lethal – in the US , one third of patients will die from this disease within five years of d...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biotechnology", "medicine", "applied", "mathematics", "cancers", "and", "neoplasms", "hematologic", "cancers", "and", "related", "disorders", "biomedical", "engineering", "oncology", "mathematics", "lymphomas", "bioengineering", "non-hodgkin", "lymphoma", "computer", "scie...
2013
An Integrated Computational/Experimental Model of Lymphoma Growth
The ventral striatum ( VS ) is a central node within a distributed network that controls appetitive behavior , and neuromodulation of the VS has demonstrated therapeutic potential for appetitive disorders . Local field potential ( LFP ) oscillations recorded from deep brain stimulation ( DBS ) electrodes within the VS ...
As neuropsychiatry begins to leverage the power of computational methods to understand disease states and to develop better therapies , it is vital that we acknowledge the trade-offs between model complexity and performance . We show that computational methods can elucidate a neural signature of feeding behavior and we...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "vertebrates", "diet", "neuroscience", "animals", "mammals", "surgical", "and", "invasive", "medical", "procedures", "animal", "models", "physiological", "processes", "model", "organisms", "nutrition", "functional...
2019
Finding the balance between model complexity and performance: Using ventral striatal oscillations to classify feeding behavior in rats
Myelination is essential for rapid impulse conduction in the CNS , but what determines whether an individual axon becomes myelinated remains unknown . Here we show , using a myelinating coculture system , that there are two distinct modes of myelination , one that is independent of neuronal activity and glutamate relea...
Myelination acts as an insulator for neurons and as such is essential for normal brain function , ensuring fast neuronal communication . Oligodendrocytes are the cells that wrap their membrane around nerve cell axons to form the myelin sheath that enables fast action potential propagation . However , what determines wh...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[ "neuroglial", "development", "neurobiology", "of", "disease", "and", "regeneration", "developmental", "neuroscience", "ion", "channels", "cellular", "neuroscience", "neural", "stem", "cells", "neurotransmitters", "biology", "neuroscience" ]
2013
Neuregulin and BDNF Induce a Switch to NMDA Receptor-Dependent Myelination by Oligodendrocytes
Analysis of mycobacterial strains that have lost their ability to cause disease is a powerful approach to identify yet unknown virulence determinants and pathways involved in tuberculosis pathogenesis . Two of the most widely used attenuated strains in the history of tuberculosis research are Mycobacterium bovis BCG ( ...
Mycobacterium tuberculosis , the causative agent of human tuberculosis is an extremely successful human pathogen in spite of its lack of classical virulence factors , such as toxins . The pathogenesis of this bacterium is closely linked with its ability to circumvent destruction by the host cell , which depends on a la...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology", "infectious", "diseases", "microbiology", "eubacteria" ]
2008
Control of M. tuberculosis ESAT-6 Secretion and Specific T Cell Recognition by PhoP
Hookworms infect millions of people worldwide and can cause severe clinical symptoms in their hosts . Prospective cohort studies in Brazil show high rates of hookworm reinfection in malnourished children compared to well-nourished children , despite previous treatment . Additionally , soil-transmitted helminth ( STH ) ...
Hookworm's infection is a health problem that affects areas of poverty worldwide . Nutritional diseases , including dietary protein deficiency are also prevalent in those areas . It is well known that nutritional status can influence parasite infection , reducing host responses to infection . However , the influence of...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "nutrition", "veterinary", "parasitology", "biology", "and", "life", "sciences", "diet", "intestinal", "parasites", "malnutrition", "parasitology" ]
2014
Evaluation of Biochemical, Hematological and Parasitological Parameters of Protein-Deficient Hamsters Infected with Ancylostoma ceylanicum
Despite estimates that , each year , as many as 300 million dengue virus ( DENV ) infections result in either no perceptible symptoms ( asymptomatic ) or symptoms that are sufficiently mild to go undetected by surveillance systems ( inapparent ) , it has been assumed that these infections contribute little to onward tr...
Most dengue virus infections result in either no perceptible symptoms or symptoms that are so mild that they go undetected by surveillance systems . It is unclear how much these infections contribute to the overall transmission and burden of dengue . At an individual level , we show that people with asymptomatic infect...
[ "Abstract", "Introduction", "Results", "Discussion", "Conclusion", "Materials", "and", "methods" ]
[ "invertebrates", "dengue", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "vector-borne", "diseases", "microbiology", "geographical", "locations", "animals", "viruses", "rna", "viruses", "infectio...
2018
Contributions from the silent majority dominate dengue virus transmission
In large collections of tumor samples , it has been observed that sets of genes that are commonly involved in the same cancer pathways tend not to occur mutated together in the same patient . Such gene sets form mutually exclusive patterns of gene alterations in cancer genomic data . Computational approaches that detec...
Tumor DNA carries multiple alterations , including somatic point mutations , amplifications , and deletions . It is challenging to identify the disease-causing alterations from the plethora of random ones , and to delineate their functional relations and involvement in common pathways . One solution for this task is in...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "mathematics", "statistics", "(mathematics)", "genome", "analysis", "genetics", "statistical", "methods", "biology", "and", "life", "sciences", "physical", "sciences", "genomics", "computational", "biology" ]
2014
Modeling Mutual Exclusivity of Cancer Mutations
A group of children aged 6–17 years was recruited and followed up for 12 months to study the impact of schistosome infection on malaria parasite prevalence , density , distribution and anemia . Levels of cytokines , malaria specific antibodies in plasma and parasite growth inhibition capacities were assessed . Baseline...
Malaria and schistosomiasis are the most prevalent tropical diseases in sub-Saharan Africa and together exert a huge burden of mortality and morbidity . The geographical overlap of these diseases among the individuals and at the population level commonly occurs resulting inevitably in frequent co-infections . It is not...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "microbiology/immunity", "to", "infections", "infectious", "diseases/neglected", "tropical", "diseases", "infectious", "diseases/helminth", "infections", "public", "health", "and", "epidemiology/infectious", "diseases", "microbiology/parasitology", "infectious", "diseases/protozoal...
2010
Impact of Schistosome Infection on Plasmodium falciparum Malariometric Indices and Immune Correlates in School Age Children in Burma Valley, Zimbabwe
Calmodulin is a calcium-binding protein ubiquitous in eukaryotic cells , involved in numerous calcium-regulated biological phenomena , such as synaptic plasticity , muscle contraction , cell cycle , and circadian rhythms . It exibits a characteristic dumbell shape , with two globular domains ( N- and C-terminal lobe ) ...
Calmodulin , the ubiquitous calcium-activated second messenger in eukaryotes , is an extremely versatile molecule involved in many biological processes: muscular contraction , synaptic plasticity , circadian rhythm , and cell cycle , among others . The protein is structurally organised into two globular lobes , joined ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Modulation of Calmodulin Lobes by Different Targets: An Allosteric Model with Hemiconcerted Conformational Transitions
Studying ion channel currents generated distally from the recording site is difficult because of artifacts caused by poor space clamp and membrane filtering . A computational model can quantify artifact parameters for correction by simulating the currents only if their exact anatomical location is known . We propose th...
The study of ion channels is essential both for understanding normal brain function and for finding drug targets to treat neurological disease . Traditional experimental techniques remain challenging for recording ion channel currents accurately because of their locations in the neuron . Computer modeling of the three ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Distal Spike Initiation Zone Location Estimation by Morphological Simulation of Ionic Current Filtering Demonstrated in a Novel Model of an Identified Drosophila Motoneuron
Circulating monocyte sub-sets have recently emerged as mediators of divergent immune functions during infectious disease but their role in helminth infection has not been investigated . In this study we evaluated whether ‘classical’ ( CD14brightCD16− ) , ‘intermediate’ ( CD14brightCD16+ ) , and ‘non-classical’ ( CD14di...
The parasite Schistosoma infects over 200 million people world-wide and can cause serious morbidity . Infection occurs following exposure to larvae ( cercariae ) which release excretory/secretory ( E/S ) material to aid their entry into exposed skin . Larvae mature into adult worms that produce hundreds of eggs per day...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "helminth", "infections", "infectious", "diseases", "schistosomiasis", "medicine", "and", "health", "sciences", "neglected", "tropical", "diseases", "tropical", "diseases", "parasitic", "diseases" ]
2014
Circulating CD14brightCD16+ ‘Intermediate’ Monocytes Exhibit Enhanced Parasite Pattern Recognition in Human Helminth Infection
Leukocyte Adhesion Deficiency I ( LAD-I ) is a primary immunodeficiency caused by single gene mutations in the CD18 subunit of β2 integrins which result in defective transmigration of neutrophils into the tissues . Affected patients suffer from recurrent life threatening infections and severe oral disease ( periodontit...
Leukocyte adhesion deficiency ( LAD ) is a primary immunodeficiency resulting from gene mutations in the CD18 subunit of β2 integrins that lead to defective neutrophil adhesion and transmigration into tissues . Affected patients suffer from recurrent life threatening infections and from a severe form of the oral diseas...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Subgingival Microbial Communities in Leukocyte Adhesion Deficiency and Their Relationship with Local Immunopathology
Many pathogens are able to manipulate the signaling pathways responsible for the generation of host immune responses . Here we examine and model a respiratory infection system in which disruption of host immune functions or of bacterial factors changes the dynamics of the infection . We synthesize the network of intera...
The immune response is a complex network of processes activated in a host upon infection . Pathogens seek to disrupt or evade these processes to ensure their own survival and proliferation . This article provides a systems-level analysis of the immune response against two related bacterial species in the Bordetella gen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "infectious", "diseases", "immunology", "computational", "biology", "mus", "(mouse)", "eubacteria" ]
2007
Modeling Systems-Level Regulation of Host Immune Responses
DNA Damage Tolerance ( DDT ) mechanisms help dealing with unrepaired DNA lesions that block replication and challenge genome integrity . Previous in vitro studies showed that the bacterial replicase is able to re-prime downstream of a DNA lesion , leaving behind a single-stranded DNA gap . The question remains of what ...
DNA Damage Tolerance ( DDT ) mechanisms help dealing with unrepaired DNA lesions that block replication , thus challenging genome integrity . Two DDT mechanisms have previously been described: error prone Translesion Synthesis operated by specialized DNA polymerases and error free bypass that uses the information of th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Bacterial Proliferation: Keep Dividing and Don't Mind the Gap
The evolutionary stability of cooperative traits , that are beneficial to other individuals but costly to their carrier , is considered possible only through the establishment of a sufficient degree of assortment between cooperators . Chimeric microbial populations , characterized by simple interactions between unrelat...
Although pervasive in the living world , collective behavior is a puzzle for evolutionary biology . The genetic traits that sustain it are costly for their carriers and make them vulnerable to the exploitation of asocial “free-riders” that benefit from the group without contributing to its cohesion . This paradox has s...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "organismal", "evolution", "evolutionary", "selection", "computerized", "simulations", "algorithms", "mutation", "population", "modeling", "microbial", "evolution", "population", "biology", "emergence", "theoretical", "biology", "evolutionary", "modeling", "biology", "evoluti...
2014
Differential Adhesion between Moving Particles as a Mechanism for the Evolution of Social Groups
Plasmodium falciparum is the protozoan parasite that causes the most virulent of human malarias . The blood stage parasites export several hundred proteins into their host erythrocyte that underlie modifications linked to major pathologies of the disease and parasite survival in the blood . Unfortunately , most are ‘hy...
Plasmodium falciparum , the most virulent form of human malaria , causes disease when it invades a red blood cell . It sends proteins beyond its borders into the host , changing the red cell to make it a suitable environment to live in and to interact with the host immune system . Recent findings have predicted that hu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "genetics", "and", "genomics/gene", "expression", "cell", "biology/membranes", "and", "sorting", "microbiology/parasitology", "infectious", "diseases/protozoal", "infections", "infectious", "diseases/tropical", "and", "travel-associated", ...
2008
An Erythrocyte Vesicle Protein Exported by the Malaria Parasite Promotes Tubovesicular Lipid Import from the Host Cell Surface
Directional migration of neural crest ( NC ) cells is essential for patterning the vertebrate embryo , including the craniofacial skeleton . Extensive filopodial protrusions in NC cells are thought to sense chemo-attractive/repulsive signals that provide directionality . To test this hypothesis , we generated null muta...
During vertebrate embryogenesis , neural crest ( NC ) cells migrate extensively along stereotypical migration routes and differentiate into diverse derivatives , including the craniofacial skeleton and peripheral nervous system . While defects in NC migration underlie many human birth defects and may be coopted during ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Fascin1-Dependent Filopodia are Required for Directional Migration of a Subset of Neural Crest Cells
HIV-1 groups M and N emerged within the last century following two independent cross-species transmissions of SIVcpz from chimpanzees to humans . In contrast to pandemic group M strains , HIV-1 group N viruses are exceedingly rare , with only about a dozen infections identified , all but one in individuals from Cameroo...
Differences in their degree of adaptation to humans may explain why only one of four ape-derived SIV zoonoses spawned the AIDS pandemic . Specifically , only HIV-1 strains of the pandemic M group evolved a fully functional Vpu that efficiently antagonizes human tetherin and degrades CD4 . In comparison , the rare group...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "zoonoses", "sexually", "transmitted", "diseases", "viral", "diseases" ]
2012
Human Tetherin Exerts Strong Selection Pressure on the HIV-1 Group N Vpu Protein
Sex reversal can occur in XY humans with only a single functional WT1 or SF1 allele or a duplication of the chromosome region containing WNT4 . In contrast , XY mice with only a single functional Wt1 , Sf1 , or Wnt4 allele , or mice that over-express Wnt4 from a transgene , reportedly are not sex-reversed . Because gen...
It has been proposed that mice do not adequately model human disorders of sex development because testis determination is gene dosage-sensitive in humans , but initial studies suggested it is gene dosage-insensitive in mice . For example , XY humans with reduced functional WT1 or SF1 gene-dosage or increased WNT4 gene-...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "morphogenesis", "genetics", "and", "genomics" ]
2012
Sex Reversal in C57BL/6J XY Mice Caused by Increased Expression of Ovarian Genes and Insufficient Activation of the Testis Determining Pathway
Macrophages are a diverse group of phagocytic cells acting in host protection against stress , injury , and pathogens . Here , we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells , and important for production of type I interferon . Scavenge...
Macrophages are a diverse group of phagocytic cells acting in host protection against stress , injury , and pathogens . They phenotypically and functionally adapt to their local environment , for example , peritoneal macrophages are distinct from brain-resident microglia , from liver-resident Kupffer cells or lung macr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "binding", "blood", "cells", "cell", "physiology", "transfection", "medicine", "and", "health", "sciences", "chemical", "characterization", "immune", "physiology", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "micro...
2018
Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor
Characterizing the activating and inhibiting effect of protein-protein interactions ( PPI ) is fundamental to gain insight into the complex signaling system of a human cell . A plethora of methods has been suggested to infer PPI from data on a large scale , but none of them is able to characterize the effect of this in...
Mathematical models which aim to describe cellular signaling start from constructing an interaction network of effectors , mediators and their effected target proteins . Several developments came up making it easier to put these links together . Besides tediously assembling knowledge from textbooks and research article...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "systems", "biology", "biology", "and", "life", "sciences", "computational", "biology" ]
2014
Characterizing Protein Interactions Employing a Genome-Wide siRNA Cellular Phenotyping Screen
Amoebiasis , caused by Entamoeba histolytica infection , is a global public health problem . However , available drugs to treat amoebiasis are currently limited , and no effective vaccine exists . Therefore , development of new preventive measures against amoebiasis is urgently needed . Here , to develop new drugs agai...
Amoebiasis is a parasitic disease caused by Entamoeba histolytica that is an important health problem worldwide because of high morbidity and mortality rates . However , clinical options are inadequate; therefore , developing new preventive measures , such as anti-amoebic drugs , is urgently needed . In general , for t...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "trophozoites", "parasite", "groups", "medicine", "and", "health", "sciences", "crystal", "structure", "tropical", "diseases", "condensed", "matter", "physics", "enzymology", "parasitic", "diseases", "parasitic", "protozoans", "parasitology", "apicomplexa", "amebiasis", "...
2019
Characterization of Entamoeba histolytica adenosine 5′-phosphosulfate (APS) kinase; validation as a target and provision of leads for the development of new drugs against amoebiasis
Activity in the human brain moves between diverse functional states to meet the demands of our dynamic environment , but fundamental principles guiding these transitions remain poorly understood . Here , we capitalize on recent advances in network science to analyze patterns of functional interactions between brain reg...
The human brain is a complex system in which the interactions of billions of neurons give rise to a fascinating range of behaviors . In response to its changing environment—for example , across situations involving rest , memory , focused attention , or learning—the brain dynamically switches between distinct patterns ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "computer", "and", "information", "sciences", "cognitive", "neuroscience", "neural", "networks", "network", "analysis", "computational", "neuroscience", "biology", "and", "life", "sciences", "computational", "biology", "neuroscience" ]
2015
Brain Network Adaptability across Task States
Cancer cells depend on transcription of telomerase reverse transcriptase ( TERT ) . Many transcription factors affect TERT , though regulation occurs in context of a broader network . Network effects on telomerase regulation have not been investigated , though deeper understanding of TERT transcription requires a syste...
Tumour cells acquire the ability to divide and multiply indefinitely whereas normal cells can undergo only a limited number of divisions . The switch to immortalisation of the tumour cell is dependent on maintaining the integrity of telomere DNA which forms chromosome ends and is achieved through activation of the telo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "systems", "biology", "medicine", "biology", "computational", "biology", "molecular", "cell", "biology" ]
2014
Mathematical Model of a Telomerase Transcriptional Regulatory Network Developed by Cell-Based Screening: Analysis of Inhibitor Effects and Telomerase Expression Mechanisms
T cell vaccines against Mycobacterium tuberculosis ( Mtb ) and other pathogens are based on the principle that memory T cells rapidly generate effector responses upon challenge , leading to pathogen clearance . Despite eliciting a robust memory CD8+ T cell response to the immunodominant Mtb antigen TB10 . 4 ( EsxH ) , ...
CD8+ T cells are important for enforcing latency of tuberculosis , and for Mtb control in patients with HIV and low CD4 counts . While vaccines that primarily elicit CD4+ T cell responses have had difficulty preventing active pulmonary TB , a TB vaccine that elicits a potent memory CD8+ T cells is a logical alternative...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
A Higher Activation Threshold of Memory CD8+ T Cells Has a Fitness Cost That Is Modified by TCR Affinity during Tuberculosis
Cutaneous Leishmaniasis ( CL ) is a neglected tropical vector-borne disease . Sand fly vectors ( SF ) and Leishmania spp parasites are sensitive to changes in weather conditions , rendering disease transmission susceptible to changes in local and global scale climatic patterns . Nevertheless , it is unclear how SF abun...
In this study we analyze data on sand fly ( SF ) abundance and cutaneous leishmaniasis ( CL ) cases from Panamá . We asked whether weather patterns and climatic variability could have an impact on vector abundance that is ultimately reflected in CL transmission . We found that large epidemics of CL occur during the col...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusion" ]
[ "epidemiological", "statistics", "infectious", "diseases", "veterinary", "diseases", "zoonoses", "medicine", "and", "health", "sciences", "population", "modeling", "epidemiology", "disease", "dynamics", "leishmaniasis", "protozoan", "infections", "biology", "and", "life", ...
2014
Cutaneous Leishmaniasis and Sand Fly Fluctuations Are Associated with El Niño in Panamá
The genome of P . marneffei , the most important thermal dimorphic fungus causing respiratory , skin and systemic mycosis in China and Southeast Asia , possesses 23 polyketide synthase ( PKS ) genes and 2 polyketide synthase nonribosomal peptide synthase hybrid ( PKS-NRPS ) genes , which is of high diversity compared t...
Penicillium marneffei is the most important thermal dimorphic fungus causing respiratory , skin and systemic mycosis in China and Southeast Asia . Its genome possesses a large number of polyketide synthase ( PKS ) genes , which should be responsible for synthesis of secondary metabolites such as pigments , antibiotics ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biology" ]
2012
First Discovery of Two Polyketide Synthase Genes for Mitorubrinic Acid and Mitorubrinol Yellow Pigment Biosynthesis and Implications in Virulence of Penicillium marneffei
Crohn's disease ( CD ) and celiac disease ( CelD ) are chronic intestinal inflammatory diseases , involving genetic and environmental factors in their pathogenesis . The two diseases can co-occur within families , and studies suggest that CelD patients have a higher risk to develop CD than the general population . Thes...
Celiac disease and Crohn's disease are both chronic inflammatory diseases of the digestive tract . Both of these diseases are complex genetic traits with multiple genetic and non-genetic risk factors . Recent genome-wide association ( GWA ) studies have identified some of the genetic risk factors for these diseases . I...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "discovery", "genetics", "and", "genomics/complex", "traits", "gastroenterology", "and", "hepatology/inflammatory", "bowel", "disease", "genetics", "and", "genomics/genetics", "of", "disease", "gastroenterology", "and", "hepatology/small", ...
2011
A Meta-Analysis of Genome-Wide Association Scans Identifies IL18RAP, PTPN2, TAGAP, and PUS10 As Shared Risk Loci for Crohn's Disease and Celiac Disease
Resting memory CD4+ T-cells harboring latent HIV proviruses represent a critical barrier to viral eradication . Histone deacetylase inhibitors ( HDACis ) , such as suberanilohydroxamic acid ( SAHA ) , romidepsin , and panobinostat have been shown to induce HIV expression in these resting cells . Recently , it has been ...
The advent of antiretroviral therapy has greatly improved the prognosis for HIV-infected individuals with access to care . However , current therapies are unable to cure infection , committing treated individuals to a lifetime of medication with significant economic burden . Furthermore , it has become clear that antir...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "hiv", "immunopathogenesis", "immunology", "microbiology", "immunodeficiency", "viruses", "immunomodulation", "infectious", "diseases", "white", "blood", "cells", "animal", "cells", "medical", ...
2014
Histone Deacetylase Inhibitors Impair the Elimination of HIV-Infected Cells by Cytotoxic T-Lymphocytes
The Epstein-Barr virus ( EBV ) latent-lytic switch is mediated by the BZLF1 immediate-early protein . EBV is normally latent in memory B cells , but cellular factors which promote viral latency specifically in B cells have not been identified . In this report , we demonstrate that the B-cell specific transcription fact...
Epstein-Barr virus ( EBV ) is a human herpesvirus associated with B-cell malignancies . EBV infection of cells can result in either lytic replication or latency . Memory B cells are the primary site of EBV latency within the human host , while oropharyngeal epithelial cells support the lytic form of infection . However...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "biology", "microbiology", "molecular", "cell", "biology" ]
2012
The B-Cell Specific Transcription Factor, Oct-2, Promotes Epstein-Barr Virus Latency by Inhibiting the Viral Immediate-Early Protein, BZLF1
Meiosis is a specialized form of cellular division that results in the precise halving of the genome to produce gametes for sexual reproduction . Checkpoints function during meiosis to detect errors and subsequently to activate a signaling cascade that prevents the formation of aneuploid gametes . Indeed , asynapsis of...
Meiosis results in the generation of non-identical haploid gametes and maintenance of chromosome number during sexual reproduction . Precise meiotic chromosome segregation is essential for life , and in humans errors in this process contribute to aneuploidy or failure in meiosis , which manifests as spontaneous abortio...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2011
Caenorhabditis elegans Histone Methyltransferase MET-2 Shields the Male X Chromosome from Checkpoint Machinery and Mediates Meiotic Sex Chromosome Inactivation
The dramatic range expansion of the dengue vector Aedes aegypti is associated with various anthropogenic transport activities , but little is known about the underlying mechanisms driving this geographic expansion . We longitudinally characterized infestation of different vehicle types ( cars , boats , etc . ) to estim...
The dengue vector , Aedes aegypti , is an invasive mosquito that is currently in the process of expanding geographically from urban to peri-urban and rural sites throughout Latin America . To improve our understanding of Ae . aegypti population mixing and how it is introduced to new areas , we investigated the infestat...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
River Boats Contribute to the Regional Spread of the Dengue Vector Aedes aegypti in the Peruvian Amazon
Deregulation of the translational machinery is emerging as a critical contributor to cancer development . The contribution of microRNAs in translational gene control has been established however; the role of microRNAs in disrupting the cap-dependent translation regulation complex has not been previously described . Her...
Control of gene expression on the translational level is critical for proper function of major cellular processes and deregulation of translation can promote cellular transformation . Emerging actors in this post-transcriptional gene regulation are small non-coding RNAs referred to as microRNAs ( miRNAs ) . We establis...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine" ]
2014
Down-Regulation of eIF4GII by miR-520c-3p Represses Diffuse Large B Cell Lymphoma Development
Previous studies in narcolepsy , an autoimmune disorder affecting hypocretin ( orexin ) neurons and recently associated with H1N1 influenza , have demonstrated significant associations with five loci . Using a well-characterized Chinese cohort , we refined known associations in TRA@ and P2RY11-DNMT1 and identified new ...
Narcolepsy-hypocretin deficiency results from a highly specific autoimmune attack on hypocretin cells . Recent studies have established antigen presentation by specific class II proteins encoded by ( HLA DQB1*06:02 and DQA1*01:02 ) to the cognate T cell receptor as the main disease pathway , with a role for H1N1 influe...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Genome Wide Analysis of Narcolepsy in China Implicates Novel Immune Loci and Reveals Changes in Association Prior to Versus After the 2009 H1N1 Influenza Pandemic
Tri-methylated H3 lysine 4 ( H3K4me3 ) is associated with transcriptionally active genes , but its function in the transcription process is still unclear . Point mutations in the catalytic domain of ATX1 ( ARABIDOPSIS TRITHORAX1 ) , a H3K4 methyltransferase , and RNAi knockdowns of subunits of the AtCOMPASS–like ( Arab...
We provide a definitive answer to the question regarding the role of histone H3 lysine 4 tri-methylation marks in the transcription of two ATX1-regulated genes . Despite the proven correlation between the gene transcriptional activity and the level of H3K4me3 modification on the nucleosomes , whether H3K4me3 contribute...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2012
ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes
A variety of filtering methods enable the recursive estimation of system state variables and inference of model parameters . These methods have found application in a range of disciplines and settings , including engineering design and forecasting , and , over the last two decades , have been applied to infectious dise...
Influenza , or the flu , is a significant public health burden in the U . S . that annually causes between 3 , 000 and 49 , 000 deaths . Predictions of influenza , if reliable , would provide public health officials valuable advanced warning that could aid efforts to reduce the burden of this disease . For instance , m...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "computer", "and", "information", "sciences", "mathematical", "computing", "mathematics", "statistics", "(mathematics)", "population", "modeling", "biology", "and", "life", "sciences", "computing", "methods", "infectious", "disease", "modeling", "computational", "biology", ...
2014
Comparison of Filtering Methods for the Modeling and Retrospective Forecasting of Influenza Epidemics
Over the last decade a significant number of studies have highlighted the central role of host antimicrobial ( or defence ) peptides in modulating the response of innate immune cells to pathogen-associated ligands . In humans , the most widely studied antimicrobial peptide is LL-37 , a 37-residue peptide containing an ...
The study of pharmacologically active peptides is central to the understanding of disease and the development of novel therapies . Of particular interest is the array of short cationic peptides , collectively known as antimicrobial ( or host defence ) peptides . In humans , the most widely studied host defence peptide ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "immunology", "microbiology", "parasitic", "diseases", "parasitology", "bacterial", "diseases", "protein", "structure", "zoology", "immunomodulation", "immune", "system", "proteins", "infectious", "diseases", "proteins", "biology", "immune", "response", "helmint...
2011
A Family of Helminth Molecules that Modulate Innate Cell Responses via Molecular Mimicry of Host Antimicrobial Peptides
Alveolar echinococcosis ( AE ) , caused by the metacestode of the tapeworm Echinococcus multilocularis , is a lethal zoonosis associated with host immunomodulation . T helper cells are instrumental to control the disease in the host . Whereas Th1 cells can restrict parasite proliferation , Th2 immune responses are asso...
E . multilocularis is a parasitic helminth causing the chronic human disease alveolar echinococcosis . Current disease control measures are very limited resulting in a high case-fatality rate . A transiently dominating Th1 immune response is mounted at the early phase of the infection , potentially limiting parasite pr...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2014
EmTIP, a T-Cell Immunomodulatory Protein Secreted by the Tapeworm Echinococcus multilocularis Is Important for Early Metacestode Development
The formation of a membrane-enveloped virus starts with the assembly of a curved layer of capsid proteins lining the interior of the plasma membrane ( PM ) of the host cell . This layer develops into a spherical shell ( capsid ) enveloped by a lipid-rich membrane . In many cases , the budding process stalls prior to th...
Despite intense study , the life-cycle of the HIV-1 virus continues to pose mysteries . One of these is the fact that the assembly of an HIV-1 virus along the plasma membrane ( PM ) of the host cell—the budding process—stalls prior to release of the virus . Many other important viral pathogens with a surrounding lipid ...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "membrane", "potential", "microbiology", "electrophysiology", "geometry", "membrane", "proteins", "mathematics", "cellular", "structures", "and", "organelles", "thermodynamics", "lipids", "viral", "packaging", "viral", "replication",...
2019
Gaussian curvature and the budding kinetics of enveloped viruses
Incidence of Entamoeba histolytica infection and clinical manifestations and treatment response of invasive amebiasis ( IA ) in HIV-infected patients have rarely been investigated before . At the National Taiwan University Hospital , medical records of HIV-infected patients who received a diagnosis of IA between 1994 a...
Entamoeba histolytica , morphologically identical to but genetically different from E . dispar and E . moshkovskii , is the causative agent of amebiasis . Recently there have been reports of increased risk for amebiasis among men who have sex with men ( MSM ) due to oral-anal sexual contact in several developed countri...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/protozoal", "infections", "infectious", "diseases/hiv", "infection", "and", "aids", "infectious", "diseases/neglected", "tropical", "diseases" ]
2008
Increased Risk for Entamoeba histolytica Infection and Invasive Amebiasis in HIV Seropositive Men Who Have Sex with Men in Taiwan
Early detection of Mycobacterium leprae is a key strategy for disrupting the transmission chain of leprosy and preventing the potential onset of physical disabilities . Clinical diagnosis is essential , but some of the presented symptoms may go unnoticed , even by specialists . In areas of greater endemicity , serologi...
Leprosy is a chronic infectious disease caused by Mycobacterium leprae ( M . leprae ) that can infect cells in the skin and nerves . Despite efforts to eliminate leprosy , the number of M . leprae infected individuals who develop leprosy is still substantial in the world . The diagnosis relies mainly on clinical parame...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "mycobacterium", "leprae", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "engineering", "and", "technology", "tropical", "diseases", "decision", "analysis", "bacterial", "diseases", "management", "e...
2019
A novel integrated molecular and serological analysis method to predict new cases of leprosy amongst household contacts
The extracellular bloodstream form parasite Trypanosoma brucei is supremely adapted to escape the host innate and adaptive immune system . Evasion is mediated through an antigenically variable Variant Surface Glycoprotein ( VSG ) coat , which is recycled at extraordinarily high rates . Blocking VSG synthesis triggers a...
Bloodstream form African trypanosomes infect the mammalian bloodstream as extracellular parasites , where they excel at escaping the immune system including elimination by macrophages . Key for survival is a dense Variant Surface Glycoprotein ( VSG ) coat which is antigenically varied , and which is recycled from the c...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "flow", "cytometry", "complement", "system", "medicine", "and", "health", "sciences", "swimming", "immune", "cells", "rna", "interference", "immune", "physiology", "immunology", "cell", "processes", "parasitic", "protozoans", "biomechanics", "biologica...
2016
Blocking Synthesis of the Variant Surface Glycoprotein Coat in Trypanosoma brucei Leads to an Increase in Macrophage Phagocytosis Due to Reduced Clearance of Surface Coat Antibodies
Sexually reproducing parasites , such as malaria parasites , experience a trade-off between the allocation of resources to asexual replication and the production of sexual forms . Allocation by malaria parasites to sexual forms ( the conversion rate ) is variable but the evolutionary drivers of this plasticity are poor...
Malaria parasites in the host replicate asexually and , during each replication cycle , some asexuals transform into sexual stages that enable between-host transmission . It is not understood why the rate of conversion to sexual stages varies during infections despite its importance for the severity and spread of the d...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "parasite", "groups", "plasmodium", "dose", "prediction", "methods", "gametocytes", "drugs", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "parasitology", "antimalarials", "germ", "cells", "apicomplexa...
2018
Adaptive plasticity in the gametocyte conversion rate of malaria parasites
Cholera outbreaks are proposed to propagate in explosive cycles powered by hyperinfectious Vibrio cholerae and quenched by lytic vibriophage . However , studies to elucidate how these factors affect transmission are lacking because the field experiments are almost intractable . One reason for this is that V . cholerae ...
The biological factors that control the transmission of water-borne pathogens like Vibrio cholerae during outbreaks are ill defined . In this study , a molecular analysis of the active but non-culturable ( ABNC ) state of V . cholerae provides insights into the physiology of environmental adaptation . The ABNC state , ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "microbiology/environmental", "microbiology", "infectious", "diseases", "public", "health", "and", "epidemiology", "infectious", "diseases/neglected", "tropical", "diseases", "molecular", "biology", "infectious", "diseases/epidemiology", "and", "control", "of", "infectious", ...
2008
Transmission of Vibrio cholerae Is Antagonized by Lytic Phage and Entry into the Aquatic Environment
The ecologically significant shift in developmental strategy from planktotrophic ( feeding ) to lecithotrophic ( nonfeeding ) development in the sea urchin genus Heliocidaris is one of the most comprehensively studied life history transitions in any animal . Although the evolution of lecithotrophy involved substantial ...
An important challenge in both evolutionary and developmental biology is understanding the genetic and molecular basis for the differences in phenotype that we observe between species . Although changes to regulatory interactions during development often play a critical role in this process , we lack detailed examples ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "gene", "regulation", "computational", "biology", "animals", "animal", "models", "developmental", "biology", "model", "organisms", "genome", "analysis", "echinoderms", "research", "and", "analysis", "methods", "ectoderm", "embryology", "gene", "expression...
2016
Comparative Developmental Transcriptomics Reveals Rewiring of a Highly Conserved Gene Regulatory Network during a Major Life History Switch in the Sea Urchin Genus Heliocidaris
The yellow fever virus ( YFV ) recently reemerged in the large outbreaks in Africa and Brazil , and the first imported patients into Asia have recalled the concerns of YFV evolution . Here we show phylogenomics of YFV with serial clinical samples of the 2016 YFV infections . Phylogenetics exhibited that the 2016 strain...
The first importation of infections into China in 2016 and the following outbreaks in Africa and Brazil of yellow fever virus ( YFV ) have raised again the concerns of the potential viral spread into new territories . In this study , we aimed to know the evolution dynamics of YFV by using intrahost phylogenomics and to...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "sequencing", "techniques", "taxonomy", "organismal", "evolution", "angola", "geographical", "locations", "microbiology", "genome", "sequencing", "phylogenetics", "data", "management", "phylogenetic", "analysis", "microbial", "evolution", "molecular", "biology", "techniques",...
2018
Phylogenomic analysis unravels evolution of yellow fever virus within hosts
Antimony resistance complicates the treatment of infections caused by the parasite Leishmania . Using next generation sequencing , we sequenced the genome of four independent Leishmania guyanensis antimony-resistant ( SbR ) mutants and found different chromosomal alterations including aneuploidy , intrachromosomal gene...
Drug resistance remains a major concern in leishmaniasis chemotherapy , a neglected tropical disease that causes 60 , 000 deaths around the world annually . To better understand the molecular mechanisms behind drug resistance , we selected L . guyanensis parasites resistant to antimony , the first-line drug against thi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Intrachromosomal Amplification, Locus Deletion and Point Mutation in the Aquaglyceroporin AQP1 Gene in Antimony Resistant Leishmania (Viannia) guyanensis
Investigating the emergence of a particular cell type is a recurring theme in models of growing cellular populations . The evolution of resistance to therapy is a classic example . Common questions are: when does the cell type first occur , and via which sequence of steps is it most likely to emerge ? For growing popul...
How long does it take for a treatment naive , growing bacterial colony to be able to survive exposure to a cocktail of antibiotics ? En route to multidrug resistance , what order did the drugs become impotent in ? Questions such as these that pertain to the emergence of a significant cell type in a growing population a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[]
2019
Competing evolutionary paths in growing populations with applications to multidrug resistance
After entry into target cells , retroviruses encounter the host restriction factors such as Fv1 and TRIM5α . While it is clear that these factors target retrovirus capsid proteins ( CA ) , recognition remains poorly defined in the absence of structural information . To better understand the binding interaction between ...
Host restriction factors such as TRIM5α are important for preventing cross species transmission of a variety of retroviruses . They act to block viral replication but their mode of virus recognition is poorly understood . To address this question we have developed a procedure for isolating viruses that replicate in the...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "biology", "microbiology", "evolutionary", "biology", "genetics", "and", "genomics" ]
2011
Novel Escape Mutants Suggest an Extensive TRIM5α Binding Site Spanning the Entire Outer Surface of the Murine Leukemia Virus Capsid Protein
Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis , a debilitating disease of global public health importance . These relatively large parasites are able to survive prolonged periods in the human vasculature without inducing stable blood clots around them . We show here that the intravascular li...
Schistosomiasis affects more than 200 million people worldwide and causes up to 280 , 000 deaths per year . In terms of global mortality and morbidity , this disease is the most important human helminth infection . Schistosoma mansoni parasites can live for years within human blood vessels and seem to be refractory to ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Schistosomes Enhance Plasminogen Activation: The Role of Tegumental Enolase
Persistent latent reservoir of replication-competent proviruses in memory CD4 T cells is a major obstacle to curing HIV infection . Pharmacological activation of HIV expression in latently infected cells is being explored as one of the strategies to deplete the latent HIV reservoir . In this study , we characterized th...
Combination antiretroviral therapy has greatly improved the clinical outcome of HIV infection treatment . However , latent viral reservoirs established primarily in memory CD4 T cells persist even after long periods of suppressive antiretroviral therapy , which hinders the ability to achieve a prolonged drug-free remis...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunodeficiency", "viruses", "infectious", "diseases", "medicine", "and", "health", "sciences", "medical", "microbiology", "hiv", "viral", "pathogens", "microbial", "pathogens", "biology", "and", "life", "sciences", "microbiology", "viral", "diseases" ]
2014
Histone Deacetylase Inhibitor Romidepsin Induces HIV Expression in CD4 T Cells from Patients on Suppressive Antiretroviral Therapy at Concentrations Achieved by Clinical Dosing
Reduction in ploidy to generate haploid gametes during sexual reproduction is accomplished by the specialized cell division program of meiosis . Pairing between homologous chromosomes and assembly of the synaptonemal complex at their interface ( synapsis ) represent intermediate steps in the meiotic program that are es...
Diploid organisms must produce haploid gametes prior to sexual reproduction in order to maintain a constant number of chromosomes from one generation to the next . Ploidy reduction is accomplished during meiosis and requires crossover recombination-based linkages between homologous chromosomes . Here , we manipulate ka...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Evidence That Masking of Synapsis Imperfections Counterbalances Quality Control to Promote Efficient Meiosis
Mycobacterium tuberculosis ( Mtb ) employs multiple strategies to evade host immune responses and persist within macrophages . We have previously shown that the cell envelope-associated Mtb serine hydrolase , Hip1 , prevents robust macrophage activation and dampens host pro-inflammatory responses , allowing Mtb to dela...
Mycobacterium tuberculosis ( Mtb ) faces adverse conditions within host cells and has evolved many mechanisms to adapt quickly to the hostile immune environment . We have previously shown that an Mtb factor , Hip1 , is important for Mtb virulence and for modulating host immunity . While Hip1 was predicted to be a prote...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "medicine", "and", "health", "sciences", "hydrolases", "pathology", "and", "laboratory", "medicine", "proteins", "immunity", "medical", "microbiology", "host-pathogen", "interactions", "enzymes", "chaperone", "proteins", "microbial", "pathogens", "biology", ...
2014
Mycobacterium tuberculosis Hip1 Modulates Macrophage Responses through Proteolysis of GroEL2
The unicellular parasite , Entamoeba histolytica , is exposed to numerous adverse conditions , such as nutrient deprivation , during its life cycle stages in the human host . In the present study , we examined whether the parasite virulence could be influenced by glucose starvation ( GS ) . The migratory behaviour of t...
During infection , pathogens are exposed to different environmental stresses that are mostly the consequence of the host immune defense . The most studied of these environmental stresses are the response of pathogens to nitric oxide and to hydrogen peroxide , both produced by phagocytes . In contrast , the overall know...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biology", "microbiology", "parasitology" ]
2011
Glucose Starvation Boosts Entamoeba histolytica Virulence
The CD8+ T cell effector mechanisms that mediate control of HIV-1 and SIV infections remain poorly understood . Recent work suggests that the mechanism may be primarily non-lytic . This is in apparent conflict with the observation that SIV and HIV-1 variants that escape CD8+ T cell surveillance are frequently selected ...
The interplay between viruses and the immune system cannot always be studied with current experimental techniques or commonly used mathematical models . Consequently , many important questions remain unanswered . The questions we wished to address fall into this category . Recent evidence strongly suggests that CD8+ T ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2013
Can Non-lytic CD8+ T Cells Drive HIV-1 Escape?
Cellular permissiveness to HIV infection is highly heterogeneous across individuals . Heterogeneity is also found across CD4+ T cells from the same individual , where only a fraction of cells gets infected . To explore the basis of permissiveness , we performed single-cell RNA-seq analysis of non-infected CD4+ T cells ...
CD4+ T cells are the main target of human immunodeficiency virus ( HIV ) infection . However , CD4+ T cells are not equally permissive to infection , varying between individuals and across cells isolated from the same individual . We explored cellular heterogeneity by analyzing the transcriptome profile of CD4+ T cells...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "cdna", "libraries", "hiv", "infections", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "computational", "biology", "microbiology", "biomarkers", "retroviruses", "vi...
2017
Single-cell analysis identifies cellular markers of the HIV permissive cell
Humans have a remarkable ability to simulate the minds of others . How the brain distinguishes between mental states attributed to self and mental states attributed to someone else is unknown . Here , we investigated how fundamental neural learning signals are selectively attributed to different agents . Specifically ,...
In order for people to have meaningful social interactions , they need to infer each other’s beliefs . Converging evidence from humans and nonhuman primates suggests that a person’s brain can represent a second person’s beliefs by simulating that second person’s brain activity . However , it is not known how the output...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "recreation", "learning", "decision", "making", "social", "sciences", "mathematical", "models", "neuroscience", "learning", "and", "memory", "cognitive", "psychology", "cognition", "brain", "mapping", "neuroimaging", "research", "and", "analysis", "methods", "random", "...
2018
Agent-specific learning signals for self–other distinction during mentalising
Hebbian forms of synaptic plasticity are required for the orderly development of sensory circuits in the brain and are powerful modulators of learning and memory in adulthood . During development , emergence of Hebbian plasticity leads to formation of functional circuits . By modeling the dynamics of neurotransmitter r...
Neurotransmitter release is the principal form of chemical communication in the brain . When an action potential reaches a synapse , calcium influx activates the machinery for neurotransmitter release . During early neuronal development this machinery matures such that neurotransmitter release becomes time-locked to ac...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
A Developmental Switch for Hebbian Plasticity
Patients with Herpes Simplex Virus-2 ( HSV-2 ) infection face a significantly higher risk of contracting HIV-1 . This is thought to be due to herpetic lesions serving as entry points for HIV-1 and tissue-resident CD4+ T cell counts increasing during HSV-2 lesional events . We have created a stochastic and spatial mathe...
The risk of contracting HIV-1 is significantly higher in people who have genital HSV-2 infections . Here , we put forward a new mathematical model to describe HSV-2 infection and the process of HIV-1 infection in the genital mucosa surrounding HSV-2 lesions . We determine how the characteristics of HSV-2 infection affe...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "herpes", "simplex", "virus", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "cytokines", "pathogens", "immunology", "microbiology", "ret...
2018
Effects of spatiotemporal HSV-2 lesion dynamics and antiviral treatment on the risk of HIV-1 acquisition
Trio exome sequencing has been successful in identifying genes with de novo mutations ( DNMs ) causing epileptic encephalopathy ( EE ) and other neurodevelopmental disorders . Here , we evaluate how well a case-control collapsing analysis recovers genes causing dominant forms of EE originally implicated by DNM analysis...
Trio exome sequencing and de novo mutation ( DNM ) analysis has been the main approach to discovering genes responsible for severe sporadic disorders , including a range of neurodevelopmental disorders . This approach requires sequencing parents , identifying DNMs from trio sequence data , and comparing the observed ra...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "medicine", "and", "health", "sciences", "alleles", "multivariate", "analysis", "genome", "sequencing", "gene", "sequencing", "mathematics", "statistics", "(mathematics)", "genome", "analysis", "molecular", "biology", "techniques", "discrete", ...
2017
A case-control collapsing analysis identifies epilepsy genes implicated in trio sequencing studies focused on de novo mutations
In many organisms , the regenerative capacity of tissues progressively decreases as development progresses . However , the developmental mechanisms that restrict regenerative potential remain unclear . In Drosophila , wing imaginal discs become unable to regenerate upon damage during the third larval stage ( L3 ) . Her...
While some organisms exhibit remarkable regenerative abilities throughout their life , many animals , including mammals , present limited regenerative potential that progressively decreases during development . Understanding the mechanisms underlying this progressive loss is important to devise therapeutic approaches a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "rna", "interference", "gene", "regulation", "regulatory", "proteins", "dna-binding", "proteins", "green", "fluorescent", "protein", "cloning", "cell", "differentiation", "developmental", "biology", "luminescent", "proteins", "organism", "development", "transcription", "fac...
2019
Developmental regulation of regenerative potential in Drosophila by ecdysone through a bistable loop of ZBTB transcription factors