text stringlengths 57 1.72k | relation stringclasses 125
values | h dict | t dict |
|---|---|---|---|
we examined the level of 1,2-diacylglycerol and inositol phosphates in normal and ej-h-ras-transformed balb/3t3 fibroblasts by prelabelling the cells with [ 3h ] glycerol , [ 3h ] inositol , [ 14c ] glucose , [ 14c ] arachidonic acid , and [ 14c ] palmitic acid . | NA | {
"id": "C0021554",
"name": "inositol phosphates",
"pos": [
48,
67
]
} | {
"id": "C0021547",
"name": "inositol",
"pos": [
180,
188
]
} |
we examined the level of 1,2-diacylglycerol and inositol phosphates in normal and ej-h-ras-transformed balb/3t3 fibroblasts by prelabelling the cells with [ 3h ] glycerol , [ 3h ] inositol , [ 14c ] glucose , [ 14c ] arachidonic acid , and [ 14c ] palmitic acid . | NA | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
112,
123
]
} | {
"id": "C0007634",
"name": "the cells",
"pos": [
140,
149
]
} |
insulin dramatically increased diacylglycerol labeling by [ 14c ] glucose in normal cells , whereas it did not affect ras-transformed fibroblasts . | NA | {
"id": "C0007634",
"name": "cells",
"pos": [
84,
89
]
} | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
134,
145
]
} |
conversely , platelet-derived growth factor and thrombin-stimulated normal cells to a greater extent than transformed fibroblasts . | NA | {
"id": "C0007634",
"name": "normal cells",
"pos": [
68,
80
]
} | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
118,
129
]
} |
moreover , we have shown for the first time that this kinase or a kinase that coeluted from the column with pkc could be activated by a phorbol ester , pma , in a phospholipid-free system , i.e . in the absence of any cofactor of pkc . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
136,
149
]
} | {
"id": "C0039654",
"name": "pma",
"pos": [
152,
155
]
} |
we suggest that the toxin-binding site is a glycoconjugate of the g1b type ( a polysialoganglioside or a glycoprotein-proteoglycan ) which is localized on the cytoplasmic face of the granule membrane and might directly be involved in exocytotic membrane fusion . | NA | {
"id": "C0017906",
"name": "glycoconjugate",
"pos": [
44,
58
]
} | {
"id": "C0017968",
"name": "glycoprotein",
"pos": [
105,
117
]
} |
whereas the antibody directed against the epitope common to every ifp ( intermediate filament antigen or ifa ) and located on the carboxy-terminal end of the rod domain recognizes the carboxy-terminal half , the me 101 antibody , as the present study illustrates , recognizes specifically the amino-terminal half . | NA | {
"id": "C0003316",
"name": "epitope",
"pos": [
42,
49
]
} | {
"id": "C0003320",
"name": "antigen",
"pos": [
94,
101
]
} |
the cells were dialyzed with solutions containing different concentrations of ionised ca and non-hydrolyzable gtp analogues ( gtp-gamma-s and gmp-pnp ) to activate g-proteins . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
110,
113
]
} | {
"id": "C0079469",
"name": "gtp-gamma-s",
"pos": [
126,
137
]
} |
the cells were dialyzed with solutions containing different concentrations of ionised ca and non-hydrolyzable gtp analogues ( gtp-gamma-s and gmp-pnp ) to activate g-proteins . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
110,
113
]
} | {
"id": "C0018366",
"name": "gmp-pnp",
"pos": [
142,
149
]
} |
the dna histograms showed a dna ploidy pattern of type ia or ib in the cases negative for these antigens and a non-diploid or aneuploid pattern of type ii or iii in many of the positive cases . | NA | {
"id": "C0012854",
"name": "dna",
"pos": [
4,
7
]
} | {
"id": "C0000702",
"name": "a dna",
"pos": [
26,
31
]
} |
the titres of iga against epstein-barr virus , viral capsid antigens and the titres of igg against early antigen were found to be useful markers for the diagnosis of different histopathological types of nasopharyngeal carcinoma . | NA | {
"id": "C0003342",
"name": "viral",
"pos": [
47,
52
]
} | {
"id": "C0003320",
"name": "antigen",
"pos": [
105,
112
]
} |
in 13 healthy male subjects performing an increasing work on veloergometer in conditions of resistive respiratory loads , the moments of sensation of respiratory difficulties and refusal from the work , were recorded . | NA | {
"id": "C0035204",
"name": "conditions",
"pos": [
78,
88
]
} | {
"id": "C0013404",
"name": "respiratory difficulties",
"pos": [
150,
174
]
} |
a significant and important decrease of both the number of patients having insulin antibodies and the level of insulin antibodies was observed 18 months after the switch-over . | NA | {
"id": "C0021644",
"name": "insulin antibodies",
"pos": [
75,
93
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
119,
129
]
} |
on the other hand , 3 months after the transfer , there was a significant and persistent decrease ( by half ) of non-specific immune complexes ( 39 % of the patients with porcine insulins ) which did not appear to correlate with the level of insulin antibodies . | NA | {
"id": "C0003313",
"name": "immune",
"pos": [
126,
132
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
250,
260
]
} |
male chickens ( gallus domesticus ) were treated with a single intramuscular injection of oestradiol-17 beta , then changes in the liver and plasma levels of triacylglycerol , phospholipid , nonesterified fatty acids and in the hepatic activities of acetyl-coa carboxylase and fatty acid synthetase were measured at various times after injection . | NA | {
"id": "C0015688",
"name": "nonesterified fatty acids",
"pos": [
191,
216
]
} | {
"id": "C0015684",
"name": "fatty acid",
"pos": [
277,
287
]
} |
incubation of isolated plasma membranes with [ gamma-32p ] atp in the presence of ca2+ and egta and immunochemical analysis by western blotting with an anti pp75 xenoantiserum detected the pp75 in human melanoma , neuroblastoma , ovarian carcinoma and lymphoid t cells and fibroblasts but not in b-lymphoid cells , renal carcinoma cells , peripheral blood lymphocytes and splenocytes . | NA | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
273,
284
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
331,
336
]
} |
pretreatment of some proteins ( albumin , immunoglobulin g , elastin and fibrinogen ) with hypochlorite or with the mpo-h2o2-cl- system increased their susceptibility to proteolysis by trypsin , chymotrypsin or elastase . | NA | {
"id": "C0033684",
"name": "proteins",
"pos": [
21,
29
]
} | {
"id": "C0001924",
"name": "albumin",
"pos": [
32,
39
]
} |
molar pregnancy and postmolar malignant trophoblastic disease occurred much less frequently among the igbo women of enugu than the yorubas of western nigeria . | NA | {
"id": "C0020217",
"name": "molar pregnancy",
"pos": [
0,
15
]
} | {
"id": "C2931618",
"name": "trophoblastic disease",
"pos": [
40,
61
]
} |
the protein had a molecular weight of 67 kda , an isoelectric point of 5.0 and was identified as albumin after western blotting using an antibody against rat serum albumin . | NA | {
"id": "C0033684",
"name": "protein",
"pos": [
4,
11
]
} | {
"id": "C0001924",
"name": "albumin",
"pos": [
164,
171
]
} |
albumin in this fraction gave a dose-dependent ( 0.1-2 % protein , w/v ) increase in lh-induced pregnenolone production , up to 4-fold , and the increase in specific bioactivity when compared to rtf was 1.4-fold . | NA | {
"id": "C0001924",
"name": "albumin",
"pos": [
0,
7
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
57,
64
]
} |
previous in vitro studies concerning the renal metabolism of 25-hydroxyvitamin d3 ( 25 ( oh ) d3 ) to form 1,25-dihydroxyvitamin d3 ( 1,25 ( oh ) 2d3 ) and 24,25r-dihydroxyvitamin d3 ( 24,25 ( oh ) 2d3 ) have utilized intact cell systems . | NA | {
"id": "C0006657",
"name": "25-hydroxyvitamin",
"pos": [
61,
78
]
} | {
"id": "C2713074",
"name": "25 ( oh ) d3",
"pos": [
84,
96
]
} |
to evaluate the possible role of intracellular phosphatases in the local regulation of prostatic functions , the effect of sodium orthovanadate ( vo4 ) , an inhibitor of phosphotyrosyl protein phosphatases , was studied on both protein phosphorylation and acid phosphatase activity . | NA | {
"id": "C0031678",
"name": "phosphatases",
"pos": [
47,
59
]
} | {
"id": "C0085536",
"name": "phosphotyrosyl protein phosphatases",
"pos": [
170,
205
]
} |
the phosphoproteins were separated by electrophoresis and the gels were either directly submitted to autoradiography or after an alkali treatment to reveal those proteins enriched in phosphotyrosine . | NA | {
"id": "C0031689",
"name": "phosphoproteins",
"pos": [
4,
19
]
} | {
"id": "C0033684",
"name": "proteins",
"pos": [
162,
170
]
} |
we conclude that peritoneal glucose reflects blood glucose at basal state and during variations of glycaemia , nevertheless , presenting heterogeneous kinetics . | NA | {
"id": "C0017725",
"name": "glucose",
"pos": [
28,
35
]
} | {
"id": "C0005802",
"name": "blood glucose",
"pos": [
45,
58
]
} |
in competitive insulin binding to intact cells , [ leua3 ] - , [ leub24 ] - , [ serb24 ] -insulin , and mini-proinsulin ( [ b ( 1-29 ) -ala-ala-lys-a ( 1-21 ) ] -insulin ) had the same relative binding activity in both the patient 's and the control cells , but proinsulin and igf-i were markedly less able to displace 125i-insulin in the patient 's cells . | NA | {
"id": "C0021641",
"name": "insulin",
"pos": [
15,
22
]
} | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
262,
272
]
} |
in contrast to the study in intact cells , proinsulin and igf-i as well as other insulin analogues had the same relative binding activity to bind to the partially lectin-purified insulin receptor preparations from both the patient 's and the control cells . | NA | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
43,
53
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
179,
186
]
} |
in contrast , on fibronectin , the majority of the cells becomes myosin- and desmin-negative , partially due to proliferation of undifferentiated myoblast precursor cells , partially due to dedifferentiation or modulation of myoblasts into fibroblast-like myo- cells . | NA | {
"id": "C0007634",
"name": "the",
"pos": [
31,
34
]
} | {
"id": "C0016030",
"name": "fibroblast",
"pos": [
240,
250
]
} |
loss of the myogenic phenotype on fibronectin was also observed in cloned mouse myoblasts and in cultures of a differentiating mouse satellite cell line , mm14dy , confirming that the appearance of desmin-negative cells is a result of myoblast modulation and not due simply to overgrowth by muscle fibroblasts . | NA | {
"id": "C0007634",
"name": "the",
"pos": [
8,
11
]
} | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
298,
309
]
} |
myocardial infarction is often the first event of ischaemic heart disease and usually effort angina is lacking ; | NA | {
"id": "C0027051",
"name": "myocardial infarction",
"pos": [
0,
21
]
} | {
"id": "C0151744",
"name": "the",
"pos": [
31,
34
]
} |
complementation analysis of the fs ( 2 ) revertants showed that the 24 fs ( 2 ) mutations identify 13-15 loci , calculated to be about 65-75 % of the second chromosome genes ems mutable to dominant female sterility . | NA | {
"id": "C0021361",
"name": "fs",
"pos": [
32,
34
]
} | {
"id": "C0021359",
"name": "fs",
"pos": [
71,
73
]
} |
two of the fs ( 2 ) mutations are useful tools for the dominant female sterile technique : fs ( 2 ) 1 for induction and detection of germ-line clones and fs ( 2 ) ugra for follicle cell clones . | NA | {
"id": "C0021361",
"name": "female sterile",
"pos": [
64,
78
]
} | {
"id": "C0021359",
"name": "fs",
"pos": [
154,
156
]
} |
seven of them alter egg shape , whereas the others arrest development primarily at two stages : around fertilization by five fs ( 2 ) and during cleavage divisions [ by fs ( 2 ) in three loci ] . | NA | {
"id": "C0021361",
"name": "fs",
"pos": [
125,
127
]
} | {
"id": "C0021359",
"name": "fs",
"pos": [
169,
171
]
} |
quantitative dot blot analysis of messenger rna levels of the second chromosomal locus , alcohol dehydrogenase ( adh ) , and the x chromosomal locus , rudimentary ( r ) , show that adh has reduced expression and r is partially compensated per total rna in metafemales . | NA | {
"id": "C0035696",
"name": "messenger rna",
"pos": [
34,
47
]
} | {
"id": "C0035668",
"name": "rna",
"pos": [
249,
252
]
} |
the effect of 5-bromo-2'-deoxyuridine ( budr ) radiosensitizing action on meristematic cells of wheat seedlings and human peripheral blood lymphocytes synchronized with 5-fluoro-2'-deoxyuridine at the g1-s boundary was revealed . | NA | {
"id": "C0006233",
"name": "budr",
"pos": [
40,
44
]
} | {
"id": "C0011535",
"name": "deoxyuridine",
"pos": [
181,
193
]
} |
in this case control study in consecutive prospective outpatients ( 571 cases and 1074 controls ) who had undergone diagnostic upper gastrointestinal endoscopy and routine biopsies from both antral and body mucosa , we calculated the risk of coexisting active du and/or gu in different gastritis of the antrum or body and according to grade ( superficial gastritis , mild , moderate or severe atrophic gastritis ) . | NA | {
"id": "C0017152",
"name": "gastritis",
"pos": [
286,
295
]
} | {
"id": "C0017154",
"name": "atrophic gastritis",
"pos": [
393,
411
]
} |
on the other hand , the rr of ulcer , and the rr of du in particular , was below 1 in the presence of atrophic gastritis in the gastric body , irrespective of the grade of gastritis in the antrum . | NA | {
"id": "C0017154",
"name": "atrophic gastritis",
"pos": [
102,
120
]
} | {
"id": "C0017152",
"name": "gastritis",
"pos": [
172,
181
]
} |
dosing regimens with documented effectiveness in long-term controlled studies are available for both isosorbide dinitrate and isosorbide 5-mononitrate as well as for transdermal nitroglycerin systems . | NA | {
"id": "C0022252",
"name": "isosorbide dinitrate",
"pos": [
101,
121
]
} | {
"id": "C0064079",
"name": "isosorbide 5-mononitrate",
"pos": [
126,
150
]
} |
in these cultures , treatment with iron alone did not produce porphyrin accumulation , and treatment with iron + 5-aminolevulinate caused accumulation of protoporphyrin , as did treatment with 5-aminolevulinate alone . | NA | {
"id": "C0032712",
"name": "porphyrin",
"pos": [
62,
71
]
} | {
"id": "C0033733",
"name": "protoporphyrin",
"pos": [
154,
168
]
} |
heme oxygenase , the rate-limiting enzyme for heme degradation , can be inhibited by several new synthetic metalloporphyrins . | NA | {
"id": "C0018966",
"name": "heme",
"pos": [
0,
4
]
} | {
"id": "C0025541",
"name": "metalloporphyrins",
"pos": [
107,
124
]
} |
antimitochondrial antibodies in primary biliary cirrhosis recognize both specific peptides and shared epitopes of the m2 family of antigens . | NA | {
"id": "C0003316",
"name": "epitopes",
"pos": [
102,
110
]
} | {
"id": "C0003320",
"name": "antigens",
"pos": [
131,
139
]
} |
the results provide further evidence that these antibodies reliably stain perineurial cells and that in conjunction with antisera to s-100 protein and neurofilaments , the relative contributions of the perineurial fibroblast , schwann cell and neurone can be assessed within a nerve-related tumour/lesion . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
48,
58
]
} | {
"id": "C0020960",
"name": "antisera",
"pos": [
121,
129
]
} |
the prognostic value of immunophenotyping lymphomas was assessed by studying 51 cases of high-grade non-hodgkin 's lymphoma for which long term clinical follow-up ( 14-28 years ) was available . | NA | {
"id": "C0024299",
"name": "lymphomas",
"pos": [
42,
51
]
} | {
"id": "C0024305",
"name": "non-hodgkin 's",
"pos": [
100,
114
]
} |
eleven patients had aids , 10 had persistent generalised lymphadenopathy ( pgl ) , and 10 had hiv infection without pgl ( called `` '' well '' '' ) . | NA | {
"id": "C0001175",
"name": "aids",
"pos": [
20,
24
]
} | {
"id": "C0019693",
"name": "hiv infection",
"pos": [
94,
107
]
} |
the study highlights the incidence of nervous system dysfunction in hiv infection even in people who do not have aids . | NA | {
"id": "C0019693",
"name": "hiv infection",
"pos": [
68,
81
]
} | {
"id": "C0001175",
"name": "aids",
"pos": [
113,
117
]
} |
other proteoglycan molecules , such as chondroitin sulphate , hyaluronic acid and heparin , and fibronectin , the extracellular matrix protein targeted by treponemes in parasitism of host cells and tissues neither diminished nor enhanced ldl binding by live treponemes . | NA | {
"id": "C0016055",
"name": "fibronectin",
"pos": [
96,
107
]
} | {
"id": "C0079323",
"name": "extracellular matrix protein",
"pos": [
114,
142
]
} |
no intracellular il-1 alpha or beta was identified in dc comparable to that readily demonstrated in monocytes , and il-1 release from lipopolysaccharide ( lps ) -stimulated dc was not detected in either a biological assay for il-1 or an elisa assay for il-1 beta . | NA | {
"id": "C0600251",
"name": "il-1 alpha",
"pos": [
17,
27
]
} | {
"id": "C0021755",
"name": "il-1",
"pos": [
253,
257
]
} |
furthermore , strong stimulation of allogeneic t lymphocytes by dc in the mixed leucocyte reaction ( mlr ) was noted to occur in the absence of il-1 production , and this stimulation was not inhibited by polyclonal antisera to il-1 alpha and il-1 beta , which were known to inhibit il-1-mediated thymocyte proliferation . | NA | {
"id": "C0600251",
"name": "il-1 alpha",
"pos": [
227,
237
]
} | {
"id": "C0021755",
"name": "il-1",
"pos": [
282,
286
]
} |
these characteristics of the antigen recognized by cmrf-17 and its pattern of reactivity distinguish this antibody from other monoclonal antibodies recognizing b-cell activation markers . | NA | {
"id": "C0003241",
"name": "antibody",
"pos": [
106,
114
]
} | {
"id": "C0003250",
"name": "monoclonal antibodies",
"pos": [
126,
147
]
} |
on day 5 , they were challenged with 20 microliters of 0.2 % dnfb on each left ear , and on day 33 challenged again with either painting 20 microliters of 0.2 % dnfb on their both ears or intravenous administration of 15 mg of dinitrobenzene sulphonic acid ( dnbs ) . | NA | {
"id": "C0012463",
"name": "dnfb",
"pos": [
61,
65
]
} | {
"id": "C0012459",
"name": "dinitrobenzene",
"pos": [
227,
241
]
} |
all the compounds , except one i.e. , compound iv , showed analgesic activity which was much higher than met-enkephalin and morphine in mice and inhibited electrically induced contractions of isolated guineapig ileum , [ d-ala2 , met5 ] -enkephalin-morpholide and [ d-ala2 , met5 ] -enkephalin-beta-ala-amide were the most potent analgesics and nearly 6 and 500 times as active as morphine and met-enkephalin respectively . | NA | {
"id": "C0014298",
"name": "enkephalin",
"pos": [
109,
119
]
} | {
"id": "C0014295",
"name": "met-enkephalin",
"pos": [
394,
408
]
} |
human foetal liver and skeletal muscle glycogen showed the normal branched structure while the liver glycogen was found to be unusually stable . | NA | {
"id": "C0017911",
"name": "glycogen",
"pos": [
39,
47
]
} | {
"id": "C0023902",
"name": "liver glycogen",
"pos": [
95,
109
]
} |
the role of insulin resistance and hyperinsulinemia in the etiology of fructose-induced hypertension was studied in male sprague-dawley rats . | NA | {
"id": "C0021655",
"name": "insulin resistance",
"pos": [
12,
30
]
} | {
"id": "C0020459",
"name": "hyperinsulinemia",
"pos": [
35,
51
]
} |
these data provide further support that the increase in blood pressure that occurs when normal rats are fed a high fructose diet is dependent on the ability of this intervention to cause insulin resistance and hyperinsulinemia . | NA | {
"id": "C0021655",
"name": "insulin",
"pos": [
187,
194
]
} | {
"id": "C0020459",
"name": "hyperinsulinemia",
"pos": [
210,
226
]
} |
plasma aldosterone averaged 7 +/- 1.8 ng/dl before nacl infusion in subjects infused with 0.5 ng angiotensin ii and was not significantly reduced with sodium chloride infusion . | NA | {
"id": "C0037494",
"name": "nacl",
"pos": [
51,
55
]
} | {
"id": "C0008203",
"name": "chloride",
"pos": [
158,
166
]
} |
patterns of congenital anomalies included multiple anomalies ( 37.68 % ) , cns malformations ( 13.33 % ) , alimentary tract anomalies ( 6.66 % ) , cardiovascular malformations ( 8.99 % ) , genitourinary malformation ( 6.66 % ) , limb anomalies ( 13.33 % ) , and anomalies of skin and appendages ( 13.33 % ) . | NA | {
"id": "C0000768",
"name": "congenital anomalies",
"pos": [
12,
32
]
} | {
"id": "C0000772",
"name": "multiple",
"pos": [
42,
50
]
} |
effects of pyocyanine , a phenazine dye from pseudomonas aeruginosa , on oxidative burst and bacterial killing in human neutrophils . | NA | {
"id": "C0034211",
"name": "pyocyanine",
"pos": [
11,
21
]
} | {
"id": "C0031375",
"name": "phenazine",
"pos": [
26,
35
]
} |
therefore , clones 17 and 22 are lps antibodies requiring both the lipid a and the kdo region for binding . | NA | {
"id": "C0023810",
"name": "lps",
"pos": [
33,
36
]
} | {
"id": "C0023767",
"name": "lipid a",
"pos": [
67,
74
]
} |
analysis of the membrane proteins of the attenuated mutants failed to reveal any alterations in detectable major outer membrane proteins , although colonies of two of the mutants exhibited a mucoid phenotype following growth on l-agar plates . | NA | {
"id": "C0025252",
"name": "membrane proteins",
"pos": [
16,
33
]
} | {
"id": "C0033684",
"name": "proteins",
"pos": [
128,
136
]
} |
polyclonal anti-idiotype 1073 ( anti-id 1073 ) , raised against a monoclonal antibody specific for the protective epitope ( s ) of eimeria tenella sporozoites , induced cell-mediated immune ( cmi ) responses in bursectomized chickens . | NA | {
"id": "C0021013",
"name": "idiotype",
"pos": [
16,
24
]
} | {
"id": "C0003316",
"name": "epitope",
"pos": [
114,
121
]
} |
these data show that anti-id 1073 mimics the protective epitope ( s ) of the parasite and primes chickens for protective cmi responses . | NA | {
"id": "C0021013",
"name": "anti-id 1073",
"pos": [
21,
33
]
} | {
"id": "C0003316",
"name": "epitope",
"pos": [
56,
63
]
} |
immunoabsorption of these o polysaccharides on a column of immobilized a-antigen-specific monoclonal antibody enriched polymer chains with a-antigen characteristics but did not eliminate m epitopes . | NA | {
"id": "C0003320",
"name": "antigen",
"pos": [
73,
80
]
} | {
"id": "C0003316",
"name": "epitopes",
"pos": [
189,
197
]
} |
m antigens ( m+ a- ) also possessed a unique m epitope as well as a tetrasaccharide determinant common to a-antigen structures . | NA | {
"id": "C0003316",
"name": "epitope",
"pos": [
47,
54
]
} | {
"id": "C0003320",
"name": "antigen",
"pos": [
108,
115
]
} |
the paradigm that brucella a and m epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies . | NA | {
"id": "C0003316",
"name": "epitopes",
"pos": [
35,
43
]
} | {
"id": "C0003320",
"name": "antigen",
"pos": [
104,
111
]
} |
binding specificities were rationalized in terms of prototype a- and m-antigen structures , an interpretation supported by competitive binding studies with o polysaccharides and synthetic oligosaccharide analogs of the a and m antigens . | NA | {
"id": "C0032594",
"name": "polysaccharides",
"pos": [
158,
173
]
} | {
"id": "C0028959",
"name": "oligosaccharide",
"pos": [
188,
203
]
} |
antibodies specific for the a antigen required five contiguous alpha 1,2-linked 4,6-dideoxy-4-formamido-d-mannopyranosyl residues , while antibodies with equal affinities for a or m epitopes were effectively inhibited by alpha 1,2-linked tri- or tetrasaccharides . | NA | {
"id": "C0003320",
"name": "antigen",
"pos": [
30,
37
]
} | {
"id": "C0003316",
"name": "epitopes",
"pos": [
182,
190
]
} |
a epitopes were present in the m antigen , and the discovery of isolated alpha 1,3 linkages in the a antigen suggests that m epitopes occur in all a antigens . | NA | {
"id": "C0003316",
"name": "epitopes",
"pos": [
2,
10
]
} | {
"id": "C0003320",
"name": "antigens",
"pos": [
149,
157
]
} |
sera from 48 patients with systemic sclerosis ( ssc ) and from 62 patients with other connective tissue diseases were studied for nuclear staining patterns by indirect immunofluorescence ( iif ) using touch prints of hamster liver , hep-2 and cryostat sections of monkey esophagus as substrates . | NA | {
"id": "C0036421",
"name": "systemic sclerosis",
"pos": [
27,
45
]
} | {
"id": "C0009782",
"name": "connective tissue diseases",
"pos": [
86,
112
]
} |
the second one , on a cyanogen bromide-activated nitrocellulose filter obtained after a 10-min transfer and saturation step , was incubated overnight with patient sera , and the specific antibodies bound to antigens or allergens were detected . | NA | {
"id": "C0003320",
"name": "antigens",
"pos": [
207,
215
]
} | {
"id": "C0002092",
"name": "allergens",
"pos": [
219,
228
]
} |
a series of 14 monoclonal anti-carcinoembryonic antigen ( cea , mr 180,000 ) antibodies ( mabs ) that show a strong degree of selective reactivity for human colon carcinomas versus normal adult tissues were used to construct a serological map of the cea molecule . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
77,
87
]
} | {
"id": "C0003250",
"name": "mabs",
"pos": [
90,
94
]
} |
under these conditions ril-2 promotes both anti-tumor cytotoxicity and lymphocyte growth from tumors of several histological types . | NA | {
"id": "C0027651",
"name": "tumor",
"pos": [
48,
53
]
} | {
"id": "C0027652",
"name": "tumors of",
"pos": [
94,
103
]
} |
polyclonal tcr gamma/delta+ cells were obtained by direct stimulation of peripheral blood mononuclear cells with sepharose bead-conjugated anti-tcr gamma/delta mabs and il-2 . | NA | {
"id": "C0007634",
"name": "cells",
"pos": [
28,
33
]
} | {
"id": "C1321301",
"name": "peripheral blood",
"pos": [
73,
89
]
} |
incubation of human trabecular meshwork ( htm ) and scleral fibroblast ( hs ) cells with 10 ( -7 ) m dexamethasone ( dex ) results in a 60 % inhibition of prostaglandin production . | NA | {
"id": "C0016030",
"name": "fibroblast",
"pos": [
60,
70
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
78,
83
]
} |
pain scores before and after 20 minute treatments of individual headaches as well as patient and physician global evaluations were the primary efficacy variables . | NA | {
"id": "C0030193",
"name": "pain",
"pos": [
0,
4
]
} | {
"id": "C0018681",
"name": "headaches",
"pos": [
64,
73
]
} |
because there is a great difference between rupture of a cerebral abscess into a ventricle and rupture of the ventricle into an abscess , particularly with reference to the prognosis , the pathology of the two different complications is reviewed , based on the literature . | NA | {
"id": "C0006105",
"name": "cerebral abscess",
"pos": [
57,
73
]
} | {
"id": "C0000833",
"name": "abscess",
"pos": [
128,
135
]
} |
these methods were additional infusion of 5-fu for a short period ( 4 h ) or oral administration of uft or tegafur during long-term cvi of 5-fu that alone gave a plasma 5-fu level of about 50 ng/ml . | NA | {
"id": "C0016778",
"name": "tegafur",
"pos": [
107,
114
]
} | {
"id": "C0016360",
"name": "fu",
"pos": [
171,
173
]
} |
a series of thiourea and isothiouronium salt derivatives of the aglycone of teicoplanin was prepared by reaction of the terminal amino group with isothiocyanates , followed by s-alkylation of the thiourea compounds . | NA | {
"id": "C0022258",
"name": "isothiouronium",
"pos": [
25,
39
]
} | {
"id": "C0039958",
"name": "thiourea",
"pos": [
196,
204
]
} |
thiourea compounds , due to the lack of a positively charged n-terminus group , have a 10-fold lower binding constant to ac-d-ala-d-ala , a bacterial cell-wall model , than the parent antibiotic and isothiouronium salt derivatives . | NA | {
"id": "C0039958",
"name": "thiourea",
"pos": [
0,
8
]
} | {
"id": "C0022258",
"name": "isothiouronium",
"pos": [
199,
213
]
} |
in contrast to our findings in other phagocytes , there was no evidence that active membrane transport was involved in monocyte uptake of clindamycin , erythromycin and roxithromycin . | NA | {
"id": "C0014806",
"name": "erythromycin",
"pos": [
152,
164
]
} | {
"id": "C0035891",
"name": "roxithromycin",
"pos": [
169,
182
]
} |
venous blood samples and urine samples were taken at intervals for 6 and 12 h respectively post-tablet ingestion and assayed for free ampicillin or amoxycillin concentration with bacillus subtilis as indicator organism . | NA | {
"id": "C0002680",
"name": "ampicillin",
"pos": [
134,
144
]
} | {
"id": "C0002645",
"name": "amoxycillin",
"pos": [
148,
159
]
} |
the relative risk for each antimicrobial agent was greatest with clindamycin , followed by cefotaxime , cephamandole and ceftriaxone . | NA | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
91,
101
]
} | {
"id": "C0007561",
"name": "ceftriaxone",
"pos": [
121,
132
]
} |
use of proton nuclear magnetic resonance spectroscopy and the lanthanide shift reagent , eu ( hfc ) 3 , resulted in a simple and reliable method for determination of the diastereomeric purity of trans-2-phenylcyclopropylamine sulfate ( tranylcypromine sulfate ) . | NA | {
"id": "C0040778",
"name": "trans-2-phenylcyclopropylamine",
"pos": [
195,
225
]
} | {
"id": "C0304369",
"name": "tranylcypromine sulfate",
"pos": [
236,
259
]
} |
the essential amino acid data of the formulas were compared with that of human milk for the calculation of amino acid scores ( based on the single most limiting amino acid ) . | NA | {
"id": "C0002520",
"name": "amino acid",
"pos": [
14,
24
]
} | {
"id": "C0301714",
"name": "based",
"pos": [
127,
132
]
} |
ascorbic acid and dehydroascorbic acid are extracted with sulfuric acid solution , and the dehydroascorbic acid in the extract is reduced to ascorbic acid by dithiothreitol at ph 7 . | NA | {
"id": "C0003968",
"name": "ascorbic acid",
"pos": [
0,
13
]
} | {
"id": "C0011177",
"name": "dehydroascorbic acid",
"pos": [
91,
111
]
} |
the dehydroascorbic acid content can be estimated as the difference in ascorbic acid content measured with and without reduction by dithiothreitol . | NA | {
"id": "C0011177",
"name": "dehydroascorbic acid",
"pos": [
4,
24
]
} | {
"id": "C0003968",
"name": "ascorbic acid",
"pos": [
71,
84
]
} |
two types of apoe alleles from a genomic dna library of a subject with the apoe2/e5 phenotype have been cloned . | NA | {
"id": "C0003595",
"name": "apoe",
"pos": [
13,
17
]
} | {
"id": "C0052198",
"name": "apoe2",
"pos": [
75,
80
]
} |
one of these alleles encodes an apoe isoprotein having cysteine , arginine , and cysteine at positions 112 , 145 , and 158 of mature apoe isoproteins , respectively , indicating an epsilon apoe2 allele . | NA | {
"id": "C0003595",
"name": "apoe",
"pos": [
32,
36
]
} | {
"id": "C0052198",
"name": "apoe2",
"pos": [
189,
194
]
} |
free amino groups of the sugar chains were acetylated and the reducing-end sugar residues were tagged with 2-aminopyridine under new reaction conditions in which no sialic acid residue was hydrolyzed . | NA | {
"id": "C0002531",
"name": "amino",
"pos": [
5,
10
]
} | {
"id": "C0007004",
"name": "sugar",
"pos": [
75,
80
]
} |
soybean beta-amylase was modified with 2,3-epoxypropyl alpha-d- [ u-14c ] glucopyranoside ( [ 14c ] alpha-epg ) , a radioactive affinity-labeling reagent for beta-amylase , until it lost 95 % of its enzyme activity . | NA | {
"id": "C0005194",
"name": "beta-amylase",
"pos": [
8,
20
]
} | {
"id": "C0002712",
"name": "amylase",
"pos": [
163,
170
]
} |
since the surface of the lipid particle was saturated with 7 apoe molecules , the particle seemed to require to have at least 4 apoe molecules on its surface in order to obtain high binding affinity to ldl receptor . | NA | {
"id": "C0003595",
"name": "apoe",
"pos": [
61,
65
]
} | {
"id": "C0052201",
"name": "4 apoe",
"pos": [
126,
132
]
} |
in order to elucidate the mechanism of the electron transfer reaction of mitochondrial steroid hydroxylase , the reduction reaction of cytochrome p-450scc ( p-450scc ) catalyzed by covalently cross-linked complexes between adrenodoxin reductase ( ar ) and adrenodoxin ( ad ) was studied . | NA | {
"id": "C0038311",
"name": "steroid hydroxylase",
"pos": [
87,
106
]
} | {
"id": "C0008381",
"name": "cytochrome p-450scc",
"pos": [
135,
154
]
} |
the alpha-glucosidase showed relatively high activity not only toward maltose but also toward alpha-glucans , such as shellfish glycogen , soluble starch , beta-limit dextrin , amylopectin , and amylose . | NA | {
"id": "C0017696",
"name": "glucans",
"pos": [
100,
107
]
} | {
"id": "C0017911",
"name": "glycogen",
"pos": [
128,
136
]
} |
the alpha-glucosidase showed relatively high activity not only toward maltose but also toward alpha-glucans , such as shellfish glycogen , soluble starch , beta-limit dextrin , amylopectin , and amylose . | NA | {
"id": "C0017696",
"name": "glucans",
"pos": [
100,
107
]
} | {
"id": "C0038179",
"name": "starch",
"pos": [
147,
153
]
} |
the alpha-glucosidase showed relatively high activity not only toward maltose but also toward alpha-glucans , such as shellfish glycogen , soluble starch , beta-limit dextrin , amylopectin , and amylose . | NA | {
"id": "C0038179",
"name": "starch",
"pos": [
147,
153
]
} | {
"id": "C0002728",
"name": "amylopectin",
"pos": [
177,
188
]
} |
the alpha-glucosidase showed relatively high activity not only toward maltose but also toward alpha-glucans , such as shellfish glycogen , soluble starch , beta-limit dextrin , amylopectin , and amylose . | NA | {
"id": "C0038179",
"name": "starch",
"pos": [
147,
153
]
} | {
"id": "C0002732",
"name": "amylose",
"pos": [
195,
202
]
} |
this substitution converts codon 2153 from glutamine ( caa ) in apo b100 mrna to a stop codon ( uaa ) in apob48 mrna ( powell , l. m. , wallis , s. c. , pease , r. j. , edwards , y. h. , knott , t. j. , and scott , j . ( 1987 ) cell 50 , 831-840 ) . | NA | {
"id": "C0009221",
"name": "codon",
"pos": [
27,
32
]
} | {
"id": "C0242611",
"name": "stop codon",
"pos": [
83,
93
]
} |
in contrast , our studies have shown that macrophages may process some incoming ligands in endosomes ( diment , s. , and stahl , p. d. ( 1985 ) j. biol . chem . 260 , 15311-15317 ) and that cathepsin d , an aspartyl protease , is localized in these organelles ( diment , s. , leech , m. s. , and stahl , p. d. ( 1988 ) j. biol . chem . 263 , 6901-6907 ) . | NA | {
"id": "C0007427",
"name": "cathepsin d",
"pos": [
190,
201
]
} | {
"id": "C0078989",
"name": "aspartyl protease",
"pos": [
207,
224
]
} |
the genomic organization of cathepsin g is similar to that of human neutrophil elastase , rat mast cell protease ii , murine adipsin , and murine cytotoxic t-cell serine proteases , with protease catalytic residues located near the borders of exons 2 , 3 , and 5 . | NA | {
"id": "C0147080",
"name": "mast cell protease ii",
"pos": [
94,
115
]
} | {
"id": "C0036734",
"name": "serine proteases",
"pos": [
163,
179
]
} |
the genomic organization of cathepsin g is similar to that of human neutrophil elastase , rat mast cell protease ii , murine adipsin , and murine cytotoxic t-cell serine proteases , with protease catalytic residues located near the borders of exons 2 , 3 , and 5 . | NA | {
"id": "C0036734",
"name": "serine proteases",
"pos": [
163,
179
]
} | {
"id": "C0030946",
"name": "protease",
"pos": [
187,
195
]
} |
formation of intrasubunit disulfide bonds was not sufficient for ligand-binding activity and is likely to precede the conformational change , which resulted in increased resistance toward trypsin , formation of highly antigenic epitopes , and association to dimers , concomitant with the acquisition of ligand-binding activity . | NA | {
"id": "C0003320",
"name": "antigenic",
"pos": [
218,
227
]
} | {
"id": "C0003316",
"name": "epitopes",
"pos": [
228,
236
]
} |
northern blot analysis of poly ( a ) + rna from nitrogen-limited p. chrysosporium cultures indicates that the level of messenger rna correlates with expression of the enzyme and its activity . | NA | {
"id": "C0035668",
"name": "rna",
"pos": [
39,
42
]
} | {
"id": "C0035696",
"name": "messenger rna",
"pos": [
119,
132
]
} |
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