text stringlengths 57 1.72k | relation stringclasses 125
values | h dict | t dict |
|---|---|---|---|
the assay of 65-66 split proinsulin does not cross-react with insulin , proinsulin or 32-33 split proinsulin . | NA | {
"id": "C0021641",
"name": "insulin",
"pos": [
28,
35
]
} | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
98,
108
]
} |
the proinsulin assay cross-reacted 66 % with 65-66 split proinsulin but not with insulin or 32-33 split proinsulin . | NA | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
4,
14
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
107,
114
]
} |
the insulin assay cross-reacted 5.3 , 62 and 5.0 % with intact proinsulin , 65-66 split proinsulin and 32-33 split proinsulin respectively . | NA | {
"id": "C0021641",
"name": "insulin",
"pos": [
4,
11
]
} | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
115,
125
]
} |
the very low concentration of 65-66 split proinsulin meant that this derivative did not interfere significantly with the specificity of the assays of proinsulin and insulin . | NA | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
42,
52
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
165,
172
]
} |
the mean concentrations of insulin , proinsulin and 32-33 split proinsulin in eight young male subjects in the fasting state were ( pm +/- s.e.m . ) | NA | {
"id": "C0021641",
"name": "insulin",
"pos": [
27,
34
]
} | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
64,
74
]
} |
the mechanism of action of the enzyme is discussed in the light of the present findings on the persistence of the oxo group in the reduced enzyme complexes , as well as of related evidence [ george & bray ( 1988 ) biochemistry 27 , 3603-3609 ] for an oxo group in the catalytic intermediate that gives the mo ( v ) e.p.r . signal known as very rapid . | NA | {
"id": "C0014442",
"name": "enzyme",
"pos": [
31,
37
]
} | {
"id": "C0026741",
"name": "enzyme complexes",
"pos": [
139,
155
]
} |
c14-24 saturated and mono-unsaturated fatty acids formed from released acetate either by synthesis de novo or by elongation of endogenous fatty acids , fatty acids formed by 2-6-carbon elongation of added substrates , and a number of water-soluble compounds , some of which were tentatively identified as the amino acids glutamine , glutamic acid and asparagine . | NA | {
"id": "C0015690",
"name": "unsaturated",
"pos": [
26,
37
]
} | {
"id": "C0015684",
"name": "fatty acids",
"pos": [
152,
163
]
} |
c14-24 saturated and mono-unsaturated fatty acids formed from released acetate either by synthesis de novo or by elongation of endogenous fatty acids , fatty acids formed by 2-6-carbon elongation of added substrates , and a number of water-soluble compounds , some of which were tentatively identified as the amino acids glutamine , glutamic acid and asparagine . | NA | {
"id": "C0017797",
"name": "glutamine",
"pos": [
321,
330
]
} | {
"id": "C0061472",
"name": "glutamic acid",
"pos": [
333,
346
]
} |
these results provide qualitative as well as quantitative evidence for a modulation by cyclic amp of the nuclear rii and c subunit levels in rat h4iie hepatoma cells , and indicate a relatively rapid but temporarily limited dibutyryl cyclic amp-induced translocation of the rii and c subunits to nuclear sites . | NA | {
"id": "C0001455",
"name": "cyclic amp",
"pos": [
87,
97
]
} | {
"id": "C0012054",
"name": "dibutyryl cyclic",
"pos": [
224,
240
]
} |
the distribution of myelin-specific proteins was also examined , and revealed that 2',3'-cyclic nucleotide 3'-phosphohydrolase ( cnpase ) i and most of the myelin basic proteins ( mbps ) were equally distributed between both fractions . | NA | {
"id": "C0026972",
"name": "myelin",
"pos": [
20,
26
]
} | {
"id": "C0014063",
"name": "myelin basic proteins",
"pos": [
156,
177
]
} |
the rat gastric mucin was pure as compared with mucin isolated without the chaotropic reagent . | NA | {
"id": "C0017135",
"name": "gastric mucin",
"pos": [
8,
21
]
} | {
"id": "C0026682",
"name": "mucin",
"pos": [
48,
53
]
} |
comparison of these sequences with other ca2+ pump sequences reveals that the class 1 isoform corresponds to the sarcoplasmic reticulum ca2+ pump of slow-twitch skeletal/cardiac muscle , whereas the class 2 protein corresponds to a ca2+ pump recently detected in non-muscle tissues . | NA | {
"id": "C0597298",
"name": "isoform",
"pos": [
86,
93
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
207,
214
]
} |
gssg selectively elutes two gsh transferases from a mixture of rat gsh transferases bound to a gsh-agarose affinity matrix . | NA | {
"id": "C0061516",
"name": "gssg",
"pos": [
0,
4
]
} | {
"id": "C0017817",
"name": "gsh",
"pos": [
95,
98
]
} |
a labile gsh transferase homodimer termed 11-11 was purified from rat testis by gsh-agarose affinity chromatography followed by anion-exchange f.p.l.c . | NA | {
"id": "C0003075",
"name": "anion",
"pos": [
128,
133
]
} | {
"id": "C0016327",
"name": "f",
"pos": [
143,
144
]
} |
as assessed both by cholera-toxin-catalysed adp-ribosylation and by immunoblotting with an anti-peptide antiserum raised against the c-terminal decapeptide of forms of gs alpha ( the alpha subunit of the stimulatory guanine nucleotide-binding protein ) , rat glioma c6 bu1 cells express two forms of gs alpha : a major 44 kda form and a much less prevalent 42 kda form . | NA | {
"id": "C0887847",
"name": "alpha subunit",
"pos": [
183,
196
]
} | {
"id": "C0086903",
"name": "gs",
"pos": [
300,
302
]
} |
however , since pmnls could not be labelled sufficiently with [ 3h ] choline , we developed an h.p.l.c . assay to quantify mass levels of phosphorylcholine after enzymic conversion to [ 32p ] cdp-choline with ctp-phosphorylcholine ( choline phosphate ) cytidylyltransferase ( ec 2.7.7.15 ) . | NA | {
"id": "C0008405",
"name": "choline",
"pos": [
69,
76
]
} | {
"id": "C0010725",
"name": "cdp-choline",
"pos": [
192,
203
]
} |
however , since pmnls could not be labelled sufficiently with [ 3h ] choline , we developed an h.p.l.c . assay to quantify mass levels of phosphorylcholine after enzymic conversion to [ 32p ] cdp-choline with ctp-phosphorylcholine ( choline phosphate ) cytidylyltransferase ( ec 2.7.7.15 ) . | NA | {
"id": "C0008405",
"name": "choline",
"pos": [
69,
76
]
} | {
"id": "C0031716",
"name": "choline",
"pos": [
233,
240
]
} |
since lambda gt11 lacks part of the open reading frame ( orf ) 221 , previously shown to encode a protein with sequence similarity to protein phosphatase-1 and protein phosphatase-2a of mammalian cells [ cohen , collins , coulson , berndt & da cruz e silva ( 1988 ) gene 69 , 131-134 ] , the results indicate that orf221 is the protein phosphatase detected in cells infected with lambda gt10 . | NA | {
"id": "C1956003",
"name": "protein phosphatase-1",
"pos": [
134,
155
]
} | {
"id": "C0031686",
"name": "protein",
"pos": [
328,
335
]
} |
when albumin or copper ( an essential component of the free radical generating systems used ) were encapsulated , protein damage was further reduced . | NA | {
"id": "C0001924",
"name": "albumin",
"pos": [
5,
12
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
114,
121
]
} |
this model system was utilized to study the effects of fsh on the quaternary structure of fsh receptor-associated gtp-binding protein by comparing the gel filtration profiles of proteoliposomes solubilized with triton x-100 after exposure to [ 3h ] gpp ( nh ) p in the presence or absence of fsh . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
114,
117
]
} | {
"id": "C0018366",
"name": "gpp ( nh ) p",
"pos": [
249,
261
]
} |
analysis of endothelin related peptides in culture supernatant of porcine aortic endothelial cells : evidence for biosynthetic pathway of endothelin-1 . | NA | {
"id": "C0079284",
"name": "endothelin",
"pos": [
12,
22
]
} | {
"id": "C0079281",
"name": "endothelin-1",
"pos": [
138,
150
]
} |
the protein is then transported via a vesicular system to the albuminal side where it is externalized to the space of disse . | NA | {
"id": "C0033684",
"name": "protein",
"pos": [
4,
11
]
} | {
"id": "C0001924",
"name": "albuminal",
"pos": [
62,
71
]
} |
dna sequence analysis revealed that this botulinum dna insert encoded an open reading frame of 35 amino acid residues of which 34 corresponded to the n-terminal residues of botulinum neurotoxin type a . | NA | {
"id": "C0006055",
"name": "botulinum",
"pos": [
41,
50
]
} | {
"id": "C0006050",
"name": "botulinum neurotoxin type a",
"pos": [
173,
200
]
} |
we made a monoclonal antibody specifically recognizing smg p25a among many ras p21-like gtp-binding proteins and investigated the tissue distribution of smg p25a by use of this antibody . | NA | {
"id": "C0003250",
"name": "monoclonal antibody",
"pos": [
10,
29
]
} | {
"id": "C0003241",
"name": "antibody",
"pos": [
177,
185
]
} |
we utilized this enzyme to generate fragments for determining the amino acid sequence of the d-aspartyl/l-isoaspartyl methyltransferase isozyme i from human erythrocytes . | NA | {
"id": "C0014442",
"name": "enzyme",
"pos": [
17,
23
]
} | {
"id": "C0022173",
"name": "isozyme",
"pos": [
136,
143
]
} |
in a panel of 10 monoclonal antibodies raised against human ldl we detected three antibodies ( named 5a , 6b , and 6e ) which recognize both apolipoprotein b-100 and b-48 . | NA | {
"id": "C0003250",
"name": "monoclonal antibodies",
"pos": [
17,
38
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
82,
92
]
} |
the purified glycoprotein revealed immunochemically most antigenic or allergenic and demonstrated homogeneous after reaction with p. juliflora pollen antiserum , characterized by gel diffusion , immunoelectrophoresis and radioallergosorbent test . | NA | {
"id": "C0003320",
"name": "antigenic",
"pos": [
57,
66
]
} | {
"id": "C0002092",
"name": "allergenic",
"pos": [
70,
80
]
} |
the canine sequence encodes a preprogastrin of 104 amino acids which consists of a signal peptide , a 37-amino acid prosegment , and a 34-amino acid gastrin sequence , followed by a glycine ( the amide donor ) , and flanked by pairs of arginine residues . | NA | {
"id": "C0002520",
"name": "amino acid",
"pos": [
51,
61
]
} | {
"id": "C0017890",
"name": "glycine",
"pos": [
182,
189
]
} |
by comparison with prior findings on the effects of both exogenous insulin and anti-insulin serum upon proinsulin synthesis , these results argue against the view that insulin would normally exert a negative feedback control upon the biosynthetic and secretory activities of islet b-cells . | NA | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
103,
113
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
168,
175
]
} |
two of the tricyclic compounds , 1,3-diamino-8-chlorobenzo [ f ] quinazoline and 1,3-diamino-9-chlorobenzo [ f ] quinazoline , proved to have activity similar to tmq and bw301u in this system . | NA | {
"id": "C0034407",
"name": "quinazoline",
"pos": [
65,
76
]
} | {
"id": "C0085176",
"name": "tmq",
"pos": [
162,
165
]
} |
the results provide further evidence for existence of a protein occurring exclusively in mitochondria , the phosphorylation of which is selectively modified by both vanadate anion and vanadyl cation in a way differing from those hitherto described . | NA | {
"id": "C0042305",
"name": "vanadate",
"pos": [
165,
173
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
174,
179
]
} |
the influence on the area and numerical density of nuclei was investigated in 5-mm-thick slices of guinea pig liver for different fixatives and variations in tissue processing : delay in fixation , air drying , degree of acidity of 10 % formalin ( = 4 % formaldehyde ) , bouin and mercury-formalin fixatives , acetone and ethanol dehydration and understretching and overstretching of the paraffin-embedded sections . | NA | {
"id": "C0016564",
"name": "formaldehyde",
"pos": [
254,
266
]
} | {
"id": "C0949307",
"name": "formalin",
"pos": [
289,
297
]
} |
no cytologic features clearly distinguished adenocarcinomas having only focal bronchioloalveolar differentiation from those with a pure or dominant bac pattern . | NA | {
"id": "C0001418",
"name": "adenocarcinomas",
"pos": [
44,
59
]
} | {
"id": "C0007120",
"name": "bac",
"pos": [
148,
151
]
} |
hapten inhibition tests between antigen and antibody showed that galactose as well as glucose were the most potent inhibitors , suggesting their involvement in the antigenic determinant . | NA | {
"id": "C0018593",
"name": "hapten",
"pos": [
0,
6
]
} | {
"id": "C0003316",
"name": "antigenic determinant",
"pos": [
164,
185
]
} |
lymphoid cell populations from non-immunized , non-obese diabetic ( nod ) mice were investigated for spontaneous t-cell reactivity in vitro to rat insulin ( identical to mouse insulin ) and to porcine insulin ( identical to mouse insulin in the immunogenic part of the a chain loop ) . | NA | {
"id": "C0724601",
"name": "porcine insulin",
"pos": [
193,
208
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
230,
237
]
} |
in vivo priming of non-diabetic nod mice with rat insulin in cfa , or of diabetic or non-diabetic nod mice with porcine insulin failed to elicit insulin-specific t-cell responses upon restimulation in vitro . | NA | {
"id": "C0724601",
"name": "porcine insulin",
"pos": [
112,
127
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
145,
152
]
} |
lymph node cells from nod mice primed with porcine insulin and treated with anti-lyt 2 antibodies and c also failed to show insulin-specific reactivity , indicating that suppression by lyt 2 cells is not involved in the non-responsiveness observed . | NA | {
"id": "C0724601",
"name": "porcine insulin",
"pos": [
43,
58
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
124,
131
]
} |
these studies confirmed our earlier finding that d10 activation by antigen : self class ii mhc is more difficult to inhibit with clonotypic fab fragments binding to three distinct clonotypic epitopes than are responses to non-self mhc . | NA | {
"id": "C0003320",
"name": "antigen",
"pos": [
67,
74
]
} | {
"id": "C0003316",
"name": "epitopes",
"pos": [
191,
199
]
} |
among the 5 cell lines containing an il-1 message , three expressed exclusively il-1 alpha , one contained traces of il-1 beta , and only one line contained both . | NA | {
"id": "C0021755",
"name": "il-1",
"pos": [
37,
41
]
} | {
"id": "C0600251",
"name": "il-1 alpha",
"pos": [
80,
90
]
} |
the mitogenic actions of igf-i are modulated by the 21-41 kda class of igf-binding proteins ( igf-bps ) . | NA | {
"id": "C0021665",
"name": "igf-i",
"pos": [
25,
30
]
} | {
"id": "C0037657",
"name": "igf",
"pos": [
94,
97
]
} |
antibody content in tlx and tbx antisera is not directed toward the classically defined hla class i or class ii antigens and is not linked to the hla gene complex . | NA | {
"id": "C0003241",
"name": "antibody",
"pos": [
0,
8
]
} | {
"id": "C0020960",
"name": "antisera",
"pos": [
32,
40
]
} |
in summary , clinically significant asa-h are present in a substantial number of infertile women undergoing ivf/et , particularly those whose infertility is unexplained . | NA | {
"id": "C0021361",
"name": "infertile women",
"pos": [
81,
96
]
} | {
"id": "C0021359",
"name": "infertility",
"pos": [
142,
153
]
} |
in 15 out of 35 cases resulting in spontaneous abortion and 15 out of the 34 pregnancies resulting in missed abortion , cytotoxic activity of the lymphocytes during pregnancy was higher than normal . | NA | {
"id": "C0000786",
"name": "spontaneous",
"pos": [
35,
46
]
} | {
"id": "C0000814",
"name": "missed abortion",
"pos": [
102,
117
]
} |
this paper reports the determination of strychnine and brucine in different parts of semen strychni ( the seeds of strychnos nux-vomica and s. pierriana ) , and makes a comparison of the contents between the crude forms and processed products of the two seeds . | NA | {
"id": "C0202474",
"name": "strychnine",
"pos": [
40,
50
]
} | {
"id": "C0006312",
"name": "different",
"pos": [
66,
75
]
} |
other interactions affect the metabolism , transport and storage of minerals , and ultimately their excretion in urine or in endogenous gut secretions . | NA | {
"id": "C0025519",
"name": "metabolism",
"pos": [
30,
40
]
} | {
"id": "C0221102",
"name": "excretion",
"pos": [
100,
109
]
} |
naloxone and naltrexone were compared with their quaternary analogues naloxone methobromide and naltrexone methobromide for efficacy in suppressing intracranial self-stimulation behavior . | NA | {
"id": "C0027358",
"name": "naloxone",
"pos": [
0,
8
]
} | {
"id": "C0027360",
"name": "naltrexone",
"pos": [
96,
106
]
} |
in the first experiment , lateral ventricular ( lv ) but not fourth ventricular ( 4v ) ang ii elicited water and saline intake , and lv but not 4v sarile , a competitive ang ii receptor blocker , reduced saline intake aroused by ip injections of 10 mg/kg furosemide and 6 mg/kg captopril . | NA | {
"id": "C0003009",
"name": "ang ii",
"pos": [
87,
93
]
} | {
"id": "C0000107",
"name": "sarile",
"pos": [
147,
153
]
} |
effects of an anesthetic concentration of ethanol ( 100 mm ) on agonist binding in the presence and absence of the guanine nucleotide gtp were also evaluated in vitro in mouse brain . | NA | {
"id": "C0018328",
"name": "guanine nucleotide",
"pos": [
115,
133
]
} | {
"id": "C0018353",
"name": "gtp",
"pos": [
134,
137
]
} |
this study suggests that neurological disorders are among the main clinical features of aids and hiv disease in africa . | NA | {
"id": "C0001175",
"name": "aids",
"pos": [
88,
92
]
} | {
"id": "C0019693",
"name": "hiv disease",
"pos": [
97,
108
]
} |
three chondroitin sulphates and five chondroitin polysulphates , with molecular weights ranging from 3000 to 30,000 daltons , were evaluated applying the mt-4 cell-culture assay for inhibition of hiv-1 replication . | NA | {
"id": "C0008466",
"name": "chondroitin sulphates",
"pos": [
6,
27
]
} | {
"id": "C0008454",
"name": "chondroitin",
"pos": [
37,
48
]
} |
the different distribution of cytochemically demonstrable enzymes : lactate dehydrogenase ( ldh , 1.1.1.27 ) , succinate dehydrogenase ( sdh , 1.3.99.1 ) , dihydrofolate reductase ( dhfr , 1.5.1.3 ) , acid phosphatase ( acp , 3.1.3.2 ) and alkaline phosphatase ( alp , 3.1.3.1 ) , has been documented in yoshida ascites hepatoma cells in vivo or stored at 80 degrees c . | NA | {
"id": "C0038615",
"name": "succinate dehydrogenase",
"pos": [
111,
134
]
} | {
"id": "C0283592",
"name": "sdh",
"pos": [
137,
140
]
} |
a positive clone was sequenced and found to code for the serine protease inhibitor alpha 1-antichymotrypsin , an acute phase serum protein . | NA | {
"id": "C0002190",
"name": "alpha 1-antichymotrypsin",
"pos": [
83,
107
]
} | {
"id": "C0001347",
"name": "acute phase",
"pos": [
113,
124
]
} |
the a4 amyloid protein is the major subunit present in the amyloid of alzheimer 's disease . | NA | {
"id": "C1456454",
"name": "amyloid protein",
"pos": [
7,
22
]
} | {
"id": "C0002716",
"name": "amyloid",
"pos": [
59,
66
]
} |
however , the association of these 2 antibodies was protective with doses lower than necessary for the monoclonal antibodies alone . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
37,
47
]
} | {
"id": "C0003250",
"name": "monoclonal antibodies",
"pos": [
103,
124
]
} |
infection with the human immunodeficiency virus ( hiv ) is often followed by a prolonged latent state , and mechanisms of maintaining latency or inducing expression from latency are active areas in aids research . | NA | {
"id": "C0019693",
"name": "infection",
"pos": [
0,
9
]
} | {
"id": "C0001175",
"name": "aids",
"pos": [
198,
202
]
} |
normal macaque pbmc infected in vitro with siv/smm ( pbj14 ) , but not with siv/smm-9 or other virus pools from pbj , formed syncytia with sup-t1 cells , whereas all isolates formed syncytia with molt-4 clone 8 cells . | NA | {
"id": "C1321301",
"name": "pbmc",
"pos": [
15,
19
]
} | {
"id": "C0007634",
"name": "cells",
"pos": [
211,
216
]
} |
as a result , the major gangliosides were simple gangliosides such as gm3 ( u 251 : 7.7 % , c6 : 84.3 % ) , gm2 ( u 251 : 32.6 % ) and spg ( u 251 : 30.0 % ) on glioma cells whereas the major neutral glycosphingolipids were cdh , cth and globoside . | NA | {
"id": "C0017082",
"name": "gangliosides",
"pos": [
24,
36
]
} | {
"id": "C0016897",
"name": "gangliosides",
"pos": [
49,
61
]
} |
after treatment with neuraminidase 2.92 nmol/mg dry weight and 3.73 nmol/mg dry weight of sialic acid were freed from u 251 cells and c6 cell , but only 8.11 % ( u 251 cell ) and 11.24 % ( c 6 cell ) of these sialic acids originated from glycolipid , and thus the major part of sialic acid might be released from glycoprotein of the cells . | NA | {
"id": "C0037025",
"name": "sialic acids",
"pos": [
209,
221
]
} | {
"id": "C0067762",
"name": "sialic acid",
"pos": [
278,
289
]
} |
from our results , it is considered that the tumor secretes hcg if the serum beta-hcg value was higher than 30 iu/l , and that it does n't secrete hcg if beta-hcg value was lower than 10 iu/l or non calculable . | NA | {
"id": "C0018062",
"name": "hcg",
"pos": [
60,
63
]
} | {
"id": "C0106132",
"name": "beta-hcg",
"pos": [
154,
162
]
} |
according to these histological findings , these cysts are classified as follows : 1 multiloculated cyst , 1 ( respiratory ) epithelial cyst , 3 ( enterogenous ) epithelial cysts , 1 ependymal lined cyst and 1 neuroectodermal cyst . | NA | {
"id": "C0014511",
"name": "epithelial cysts",
"pos": [
162,
178
]
} | {
"id": "C0010709",
"name": "cyst",
"pos": [
226,
230
]
} |
using specific , h.p.l.c.-controlled rias for angiotensin peptides , the presence of both angiotensin i and angiotensin ii in all anatomical regions of the monkey and rat heart have been demonstrated ; | NA | {
"id": "C0003018",
"name": "angiotensin",
"pos": [
46,
57
]
} | {
"id": "C0003006",
"name": "angiotensin i",
"pos": [
108,
121
]
} |
using specific , h.p.l.c.-controlled rias for angiotensin peptides , the presence of both angiotensin i and angiotensin ii in all anatomical regions of the monkey and rat heart have been demonstrated ; | NA | {
"id": "C0003018",
"name": "angiotensin",
"pos": [
46,
57
]
} | {
"id": "C0003009",
"name": "angiotensin ii",
"pos": [
108,
122
]
} |
instability of expression of major histocompatibility antigens in fibroblasts expressing activated ras oncogene : constitutive and interferon-gamma induced class i and class ii antigens in a series of clonal isolates of murine fibroblasts transformed by v-ki-ras . | NA | {
"id": "C0019628",
"name": "histocompatibility antigens",
"pos": [
35,
62
]
} | {
"id": "C0019630",
"name": "class ii antigens",
"pos": [
168,
185
]
} |
hepatocytes obtained from wistar rats by collagenase treatment were found to take up lps only when it was not linked to the polysaccharide of o-antigen . | NA | {
"id": "C0023810",
"name": "lps",
"pos": [
85,
88
]
} | {
"id": "C0069180",
"name": "o-antigen",
"pos": [
142,
151
]
} |
these results indicate that the r-form of lps which lacks the o-antigen polysaccharide is taken up and deacylated in hepatocytes , and the derived fatty acids are released into the culture medium either in the free form or after conversion to triglyceride . | NA | {
"id": "C0023810",
"name": "lps",
"pos": [
42,
45
]
} | {
"id": "C0069180",
"name": "o-antigen",
"pos": [
62,
71
]
} |
interestingly , the highest levels of procollagen mrnas were observed in young ( pretreatment control ) animals , suggesting that during pulmonary development collagen metabolism in lungs is even greater than during development of fibrosis . | NA | {
"id": "C0033235",
"name": "procollagen",
"pos": [
38,
49
]
} | {
"id": "C0009325",
"name": "collagen",
"pos": [
159,
167
]
} |
simultaneous transplantation of different parts of the oviduct , endometrium , and conjunctive should expand the usefulness of the subcutaneous model in other studies on mixed infections or immune responses to infection . | NA | {
"id": "C0275524",
"name": "mixed infections",
"pos": [
170,
186
]
} | {
"id": "C0009450",
"name": "infection",
"pos": [
210,
219
]
} |
recombinant human interferon ( ifn ) alpha-2b in chronic myelogenous leukaemia : dose dependency of response and frequency of neutralizing anti-interferon antibodies . | NA | {
"id": "C0021735",
"name": "ifn",
"pos": [
31,
34
]
} | {
"id": "C0021747",
"name": "interferon",
"pos": [
144,
154
]
} |
all cells were reactive with anti-cd4 and anti-cdw29 monoclonal antibodies ( antibody against helper inducer t cells ) but were unreactive with anti-cd45r monoclonal antibody ( antibody against suppressor inducer t cells ) . | NA | {
"id": "C0003250",
"name": "monoclonal antibodies",
"pos": [
53,
74
]
} | {
"id": "C0003241",
"name": "antibody",
"pos": [
177,
185
]
} |
by analysing pregnancy and non-pregnancy serum with two-dimensional electrophoresis ( 2-de ) and western blotting , we could demonstrate that this antiserum marketed by dakopatts ( denmark ) was capable of binding haptoglobin whereas our own ( dr p. bischof ) antibody preparation did not recognize haptoglobin . | NA | {
"id": "C0020960",
"name": "antiserum",
"pos": [
147,
156
]
} | {
"id": "C0003241",
"name": "antibody",
"pos": [
260,
268
]
} |
the incidence of class iii was 9.4 per cent , with mandibular prognathism being the commonest presentation of the malocclusion . | NA | {
"id": "C0399526",
"name": "mandibular prognathism",
"pos": [
51,
73
]
} | {
"id": "C0024636",
"name": "malocclusion",
"pos": [
114,
126
]
} |
molecular cloning of dog mast cell tryptase and a related protease : structural evidence of a unique mode of serine protease activation . | NA | {
"id": "C0147080",
"name": "mast cell tryptase",
"pos": [
25,
43
]
} | {
"id": "C0036734",
"name": "serine protease",
"pos": [
109,
124
]
} |
molecular cloning of dog mast cell tryptase and a related protease : structural evidence of a unique mode of serine protease activation . | NA | {
"id": "C0030946",
"name": "protease",
"pos": [
58,
66
]
} | {
"id": "C0036734",
"name": "serine protease",
"pos": [
109,
124
]
} |
to determine the full primary structure of the catalytic domain and precursor forms of tryptase and to gain insight into its mode of activation , we cloned cdnas coding for the complete amino acid sequence of dog mast cell tryptase and a second , possibly related , serine protease . | NA | {
"id": "C0147080",
"name": "mast cell",
"pos": [
213,
222
]
} | {
"id": "C0036734",
"name": "serine protease",
"pos": [
266,
281
]
} |
the tryptase sequence includes the essential residues of the catalytic triad and an aspartic acid at the base of the putative substrate binding pocket that confers p1 arg and lys specificity on tryptic serine proteases . | NA | {
"id": "C0147080",
"name": "tryptase",
"pos": [
4,
12
]
} | {
"id": "C0036734",
"name": "serine proteases",
"pos": [
202,
218
]
} |
additional screening of the library with a trypsinogen cdna led to the isolation and sequencing of a full-length clone apparently coding for the complete sequence of a second tryptic serine protease ( dmp ) which is only 53.4 % identical with the dog tryptase sequence but which contains an apparent signal/activation peptide also terminating in a glycine . | NA | {
"id": "C0036734",
"name": "serine protease",
"pos": [
183,
198
]
} | {
"id": "C0147080",
"name": "tryptase",
"pos": [
251,
259
]
} |
neither secretagogue influences triacylglycerol labeling , and neither induces release of [ 3h ] choline or [ 3h ] phosphocholine from islets prelabeled with [ 3h ] choline . | NA | {
"id": "C0031716",
"name": "phosphocholine",
"pos": [
115,
129
]
} | {
"id": "C0008405",
"name": "choline",
"pos": [
165,
172
]
} |
however , when membranes were incubated with dithiothreitol ( dtt ) , followed by bam , and subsequently washed , 90 % of the 0.25 nm [ 3h ] [ d-ala2 , ( me ) phe4 , gly ( ol ) 5 ] enkephalin ( dago ) binding was irreversibly inhibited as a result of the specific alkylation of a sulfhydryl group at the mu binding site . | NA | {
"id": "C0014298",
"name": "enkephalin",
"pos": [
181,
191
]
} | {
"id": "C0525773",
"name": "dago",
"pos": [
194,
198
]
} |
the mu receptor specificity of bam alkylation was demonstrated by the ability of bam alkylated membranes to still bind the delta-selective peptide [ 3h ] [ d-penicillamine2 , d-penicillamine5 ] enkephalin ( dpdpe ) and ( - ) - [ 3h ] bremazocine in the presence of mu and delta blockers , selective for kappa binding sites . | NA | {
"id": "C0014298",
"name": "enkephalin",
"pos": [
194,
204
]
} | {
"id": "C0525856",
"name": "dpdpe",
"pos": [
207,
212
]
} |
the chromophoric divalent metal ion chelators 4- ( 2-pyridylazo ) resorcinol ( par ) and 2,2',2 '' '' -terpyridine ( terpy ) are used as kinetic and spectroscopic probes to investigate in solution the scn- -induced conformational transformations of the insulin , proinsulin , and miniproinsulin hexamers ( miniproinsulin is a proinsulin analogue wherein the c-chain is replaced by a dipeptide cross-link between gly-a1 and ala-b30 ) . | NA | {
"id": "C0021641",
"name": "insuli",
"pos": [
253,
259
]
} | {
"id": "C0033362",
"name": "proinsuli",
"pos": [
263,
272
]
} |
under t6 conditions , the kinetics of the reactions between insulin , proinsulin , and miniproinsulin and a variable excess of terpy are similar and biphasic . | NA | {
"id": "C0021641",
"name": "insulin",
"pos": [
60,
67
]
} | {
"id": "C0033362",
"name": "proinsulin",
"pos": [
91,
101
]
} |
under anaerobic conditions the carbon-centered radical generated selectively at c-5 ' of deoxyribose of thymidylate residues in dna by the antitumor antibiotic neocarzinostatin reacts with misonidazole to produce a dna damage product in the form of 3'- ( formyl phosphate ) -ended dna . | NA | {
"id": "C0000702",
"name": "a dna",
"pos": [
213,
218
]
} | {
"id": "C0012854",
"name": "dna",
"pos": [
281,
284
]
} |
by use of [ 3h ] camptothecin and an equilibrium dialysis assay , the alkaloid was shown to bind reversibly to a dna-topoisomerase i complex , but not to isolated enzyme or isolated dna . | NA | {
"id": "C0006812",
"name": "camptothecin",
"pos": [
17,
29
]
} | {
"id": "C0002062",
"name": "alkaloid",
"pos": [
70,
78
]
} |
values for dihydrofolate association rate constants ( kon ) with enzyme and enzyme-nadph complexes were measured by stopped-flow techniques . | NA | {
"id": "C0014442",
"name": "enzyme",
"pos": [
65,
71
]
} | {
"id": "C0026741",
"name": "enzyme",
"pos": [
76,
82
]
} |
kinetic modeling shows further that the predominant steady-state enzyme form is the enzyme-product complex ( e-p ) , which is expected to show a correspondingly large ( approximately 70 % of total copper ) epr signal for bound cu2+ . | NA | {
"id": "C0014442",
"name": "enzyme",
"pos": [
65,
71
]
} | {
"id": "C0026741",
"name": "enzyme",
"pos": [
84,
90
]
} |
the encoded protein sequence is also longer by one amino acid at its c-terminal end than the protein isolated from f1-atpase , but this alanine residue may have been removed artifactually during release of the f1-atpase particle from the inner mitochondrial membrane . | NA | {
"id": "C0002520",
"name": "amino acid",
"pos": [
51,
61
]
} | {
"id": "C0001898",
"name": "alanine",
"pos": [
136,
143
]
} |
thus , a concept of protein antigenicity emerges that involves the active , attractive contributions mediated by the energetic antigenic epitopes and the passive surface complementarity contributed by the surrounding contact area . | NA | {
"id": "C0003320",
"name": "antigenic",
"pos": [
28,
37
]
} | {
"id": "C0003316",
"name": "epitopes",
"pos": [
137,
145
]
} |
the assay system consisted of polystyrene balls with immobilized antibody f ( ab ' ) 2 fragments and the same antibody fab ' fragments labeled with beta-d-galactosidase from escherichia coli . | NA | {
"id": "C0021009",
"name": "antibody",
"pos": [
65,
73
]
} | {
"id": "C0021031",
"name": "antibody fab",
"pos": [
110,
122
]
} |
amyloid a is derived by proteolysis from serum amyloid a component ( saa ) , a major acute-phase reactant in many species . | NA | {
"id": "C0002723",
"name": "saa",
"pos": [
69,
72
]
} | {
"id": "C0001347",
"name": "acute-phase reactant",
"pos": [
85,
105
]
} |
the results are consistent with a model in which the photoreaction is influenced by the binding of an anion ( either chloride or nitrate ) to site ii in hr : when an anion is bound , the hrk-initiated hrl-type photocycle is observed , but when the site is not occupied , the hr ' k-initiated hro-type photocycle is seen . | NA | {
"id": "C0008203",
"name": "chloride",
"pos": [
117,
125
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
166,
171
]
} |
the results are consistent with a model in which the photoreaction is influenced by the binding of an anion ( either chloride or nitrate ) to site ii in hr : when an anion is bound , the hrk-initiated hrl-type photocycle is observed , but when the site is not occupied , the hr ' k-initiated hro-type photocycle is seen . | NA | {
"id": "C0028125",
"name": "nitrate",
"pos": [
129,
136
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
166,
171
]
} |
the individual hydrogen ion equilibria of 22-26 histidyl residues of hemoglobin have been measured in anion-free 0.1 m hepes buffer and in the presence of 0.18 m chloride or 0.1 m inorganic phosphate ions in both deoxy and carbonmonoxy forms . | NA | {
"id": "C0003075",
"name": "anion",
"pos": [
102,
107
]
} | {
"id": "C0008203",
"name": "chloride",
"pos": [
162,
170
]
} |
the individual hydrogen ion equilibria of 22-26 histidyl residues of hemoglobin have been measured in anion-free 0.1 m hepes buffer and in the presence of 0.18 m chloride or 0.1 m inorganic phosphate ions in both deoxy and carbonmonoxy forms . | NA | {
"id": "C0003075",
"name": "anion",
"pos": [
102,
107
]
} | {
"id": "C0031603",
"name": "inorganic phosphate ions",
"pos": [
180,
204
]
} |
down-regulation of protein kinase c with prolonged phorbol ester ( 12-o-tetradecanoylphorbol 13-acetate ( tpa ] treatment leads to a refractory state in which the cells do not respond to pth ( 10 ( -8 ) m ) , camp ( 10 ( -4 ) m ) or rechallenge of tpa ( 200 nm ) even though na+/pi cotransport is similar to control cells ( 8.1 +/- 0.1 nmol.mg-1 protein.5 min-1 ) . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
51,
64
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol 13-acetate",
"pos": [
67,
103
]
} |
the phorbol ester pma ( 10 nm ) caused a transient rise in calcium and inhibited the calcium rise caused by acetylcholine : it did not modify the effect of acetylcholine on inositol phosphates . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
4,
17
]
} | {
"id": "C0039654",
"name": "pma",
"pos": [
18,
21
]
} |
the affinity purified antibody , when added to cultured heart cells , increased the beat rate 40-80 % depending on the titer of the antiserum . | NA | {
"id": "C0003241",
"name": "antibody",
"pos": [
22,
30
]
} | {
"id": "C0020960",
"name": "antiserum",
"pos": [
132,
141
]
} |
in the li+ and choline medium , stimulation of the pancreas by carbachol or cck-8 increases the fluid secretion rate . | NA | {
"id": "C0008405",
"name": "choline",
"pos": [
15,
22
]
} | {
"id": "C0006945",
"name": "carbachol",
"pos": [
63,
72
]
} |
synaptosomal membrane potential , as estimated from synaptosomal accumulation of the permeant cation [ 3h ] tetraphenylphosphonium , was not affected by n-alcohols in the concentrations used in this study , suggesting that neither the inhibitory or enhancing effects of these n-alcohols were attributable to changes in trans-synaptosomal membrane ion gradients . | NA | {
"id": "C0007447",
"name": "cation",
"pos": [
94,
100
]
} | {
"id": "C0022023",
"name": "ion",
"pos": [
347,
350
]
} |
the highly ordered lipids in the prostasome membrane thus seemed to be minimally perturbed by proteins in the membrane and esr spectra showed no signs of immobilized lipids . | NA | {
"id": "C0025249",
"name": "lipids",
"pos": [
19,
25
]
} | {
"id": "C0023779",
"name": "lipids",
"pos": [
166,
172
]
} |
the analytical data for the quenching of tryptophan fluorescence by i- anion suggest that the tryptophan residue in the peptide is not deeply buried in the hydrophobic core of the bilayers . | NA | {
"id": "C0021966",
"name": "i",
"pos": [
10,
11
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
71,
76
]
} |
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