text stringlengths 57 1.72k | relation stringclasses 125
values | h dict | t dict |
|---|---|---|---|
moreover , analysis of the inhibition of the caseinolytic activity of papain by these kininogens indicated that hmwk , lmwk , and kinin-free derivatives obtained from both kininogens inhibit active papain with a stoichiometry of 1:1 . | NA | {
"id": "C0023922",
"name": "lmwk",
"pos": [
119,
123
]
} | {
"id": "C0022713",
"name": "kininogens",
"pos": [
172,
182
]
} |
on alkaline urea-polyacrylamide gel electrophoresis , the mr 52,000 component as well as rabbit troponin-i was found to form a stable complex with troponin-c or calmodulin in the presence of ca2+ . | NA | {
"id": "C0077401",
"name": "troponin-i",
"pos": [
96,
106
]
} | {
"id": "C0041199",
"name": "complex",
"pos": [
134,
141
]
} |
the sequence homology with human non-secretory rnase , bovine pancreatic rnase , and human secretory rnase are 46 , 34.6 , and 32.3 % , respectively . | NA | {
"id": "C0035541",
"name": "pancreatic rnase",
"pos": [
62,
78
]
} | {
"id": "C0014241",
"name": "rnase",
"pos": [
101,
106
]
} |
the bovine kidney rnase has two inserted sequences , a tripeptide at the n-terminus and a heptapeptide between the 113th and 114th position of bovine pancreatic rnase ; | NA | {
"id": "C0014241",
"name": "rnase",
"pos": [
18,
23
]
} | {
"id": "C0035541",
"name": "pancreatic rnase",
"pos": [
150,
166
]
} |
to identify factors potentially influencing expression of type 1 plasminogen activator inhibitor ( pai-1 ) , we characterized the human tissue-specific distribution of pai-1 mrna and the influence of epidermal growth factor ( egf ) on expression of steady state levels of pai-1 mrna and secretion of pai-1 by hep g2 cells . | NA | {
"id": "C0030190",
"name": "type 1 plasminogen",
"pos": [
58,
76
]
} | {
"id": "C0032145",
"name": "pai-1",
"pos": [
300,
305
]
} |
we have tested whether growth factors added to serum-deprived balb/c 3t3 fibroblasts alter the casein kinase ii activity measured in cell extracts . | NA | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
73,
84
]
} | {
"id": "C0007634",
"name": "cell",
"pos": [
133,
137
]
} |
these results demonstrate that casein kinase ii is a selective target for insulin and igf-i action in balb/c fibroblasts , consistent with the hypothesis that this kinase plays a role in cellular signaling by these hormones . | NA | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
109,
120
]
} | {
"id": "C0007634",
"name": "cellular",
"pos": [
187,
195
]
} |
in the two strains , inactivation of lincomycin gave rise to lincomycin 3- ( 5'-adenylate ) , whereas clindamycin was inactivated through its conversion to clindamycin 4- ( 5'-adenylate ) . | NA | {
"id": "C0023726",
"name": "lincomycin",
"pos": [
37,
47
]
} | {
"id": "C0008947",
"name": "clindamycin",
"pos": [
156,
167
]
} |
the gene lina ' encoding the 3-lincomycin , 4-clindamycin o-nucleotidyltransferase in s. aureus bm4611 has been sequenced and displays 93 % homology with the gene lina encoding the 3-lincomycin , 4-clindamycin o-nucleotidyltransferase found in s. haemolyticus bm4610 . | NA | {
"id": "C0023726",
"name": "lincomycin",
"pos": [
31,
41
]
} | {
"id": "C0008947",
"name": "clindamycin",
"pos": [
198,
209
]
} |
the topography of lactose permease , in native membrane vesicles and after reconstitution of the purified protein into proteoliposomes , has been investigated by labeling the membrane-embedded portions of the protein using photoactivatable , hydrophobic reagents and by labeling the exposed portions of the protein with water-soluble , electrophilic reagents . | NA | {
"id": "C0025252",
"name": "membrane",
"pos": [
47,
55
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
307,
314
]
} |
the apparent lack of reactivity of some of these residues may be explained either by their being buried in the protein moiety within the membrane domain , or by their close association with other ionizable residues on the surface of the protein . | NA | {
"id": "C0033684",
"name": "protein",
"pos": [
111,
118
]
} | {
"id": "C0025252",
"name": "surface",
"pos": [
222,
229
]
} |
fraction i did not correspond to either bradykinin or other known kinins , whereas fraction ii corresponded to bradykinin . | NA | {
"id": "C0022714",
"name": "kinins",
"pos": [
66,
72
]
} | {
"id": "C0006100",
"name": "bradykinin",
"pos": [
111,
121
]
} |
fraction i contained 8 amino acid residues from bradykinin minus 1 proline plus 1 additional hydroxyproline . | NA | {
"id": "C0033382",
"name": "proline",
"pos": [
67,
74
]
} | {
"id": "C0020388",
"name": "hydroxyproline",
"pos": [
93,
107
]
} |
sequence analysis of fraction i showed that the proline at the third amino acid residue of bradykinin was replaced by hydroxyproline . | NA | {
"id": "C0033382",
"name": "proline",
"pos": [
48,
55
]
} | {
"id": "C0020388",
"name": "hydroxyproline",
"pos": [
118,
132
]
} |
the effect of varying the concentration of tyrosine on the ki value for 5-deaza-6-methyltetrahydropterin is consistent with the binding of this inhibitor to both the free enzyme and to an enzyme-dihydroxyphenylalanine complex . | NA | {
"id": "C0014442",
"name": "enzyme",
"pos": [
171,
177
]
} | {
"id": "C0026741",
"name": "enzyme",
"pos": [
188,
194
]
} |
the effect of changing the fixed substrate is consistent with the binding of this inhibitor to both the free enzyme and to the enzyme-tetrahydropterin complex . | NA | {
"id": "C0014442",
"name": "enzyme",
"pos": [
109,
115
]
} | {
"id": "C0026741",
"name": "enzyme",
"pos": [
127,
133
]
} |
our results demonstrate that calf-heart agglutinin binds with high affinity to oligosaccharides containing the repeating disaccharide ( 3gal beta 1-4glcnac beta 1 ) n or poly-n-acetyllactosamine sequence and that the presence of terminal beta-linked galactosyl residues is neither sufficient nor necessary for high affinity interactions . | NA | {
"id": "C0028959",
"name": "oligosaccharides",
"pos": [
79,
95
]
} | {
"id": "C0012611",
"name": "disaccharide",
"pos": [
121,
133
]
} |
expression of the fgf gene in this system can lead to an accumulation of approximately 40 mg/liter/a600 unit of bfgf . | NA | {
"id": "C0016026",
"name": "fgf",
"pos": [
18,
21
]
} | {
"id": "C0380603",
"name": "bfgf",
"pos": [
112,
116
]
} |
it is apparent , thus , that antibodies present in the xenogeneic antiserum could specifically bind to the il-1 receptor moiety within the complex . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
29,
39
]
} | {
"id": "C0020960",
"name": "antiserum",
"pos": [
66,
75
]
} |
we conclude that our antiserum contains antibodies that recognize determinants expressed on the following proteins : on nascent chains of il-1 binding proteins ; | NA | {
"id": "C0020960",
"name": "antiserum",
"pos": [
21,
30
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
40,
50
]
} |
phi x174 viral strand circular dna can be synthesized in vitro from phi x174 replicative form i ( rfi ) dna in the presence of the phi x a protein , the escherichia coli dna polymerase iii elongation system , the e. coli rep helicase , and the e. coli single-stranded dna binding protein . | NA | {
"id": "C0012939",
"name": "viral",
"pos": [
9,
14
]
} | {
"id": "C0012854",
"name": "dna",
"pos": [
268,
271
]
} |
from sequence homology to other aspartic proteases whose x-ray crystallographic structures are known , such as pepsinogen and penicillopepsin , it is clear that the processing regions are insertions to form an extended beta-hairpin loop between residues 91 and 92 ( porcine pepsin numbers ) . | NA | {
"id": "C0078989",
"name": "aspartic proteases",
"pos": [
32,
50
]
} | {
"id": "C0030909",
"name": "pepsin",
"pos": [
274,
280
]
} |
recent experiments showed that a single base pair ( g3 : u70 ) in the amino acid acceptor helix is a major determinant for the identity of escherichia coli alanine transfer rna . | NA | {
"id": "C0002520",
"name": "amino acid",
"pos": [
70,
80
]
} | {
"id": "C0001898",
"name": "alanine",
"pos": [
156,
163
]
} |
transfected cells grown in serum-free medium for 72 h expressed human gh measured by specific radioimmunoassay , incorporation of [ 35s ] methionine into newly synthesized gh , and the presence of the appropriately sized protected transcripts seen after rnase protection assay . | NA | {
"id": "C0169964",
"name": "human gh",
"pos": [
64,
72
]
} | {
"id": "C0037663",
"name": "gh",
"pos": [
172,
174
]
} |
compared with other membrane proteins , lac12 ( orfi ) protein showed sequence similarity to the human glucose and the escherichia coli xylose-h+ and arabinose-h+ transporters . | NA | {
"id": "C0025252",
"name": "membrane",
"pos": [
20,
28
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
55,
62
]
} |
preincubation of these cells for 20 min with isoproterenol , forskolin , 8-br-camp and 8- ( 4-cl-phenyl ) thio-camp did not lower carbachol-induced increases in cytosolic calcium concentration , whereas the phorbol ester 12-o-tetradecanoylphorbol 13-acetate , the atrionatriuretic factor , isobutylmethylxanthine , and 8-br-cgmp lowered cytosolic calcium . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
207,
220
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol 13-acetate",
"pos": [
221,
257
]
} |
however , elevation of camp simultaneously with phorbol ester treatment potentiated the phorbol ester-induced release of tpa 6 times from 22.2 ng/ml with phorbol myristate acetate ( pma ) alone to 122.1 ng/ml ( pma and forskolin ) . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
48,
61
]
} | {
"id": "C0039654",
"name": "pma",
"pos": [
211,
214
]
} |
we propose that molybdate and vanadate may exert their effects on the untransformed receptor by interacting with the binding site for the endogenous metal anion . | NA | {
"id": "C0042305",
"name": "vanadate",
"pos": [
30,
38
]
} | {
"id": "C0003075",
"name": "anion",
"pos": [
155,
160
]
} |
the intracellular concentrations of polyphosphoinositides and inositol phosphates were determined , and their role in growth factor-initiated cell division was investigated in a chinese hamster ovary cell inositol auxotroph ( cho-k1-ins ) . | NA | {
"id": "C0021554",
"name": "inositol",
"pos": [
62,
70
]
} | {
"id": "C0021547",
"name": "inositol",
"pos": [
205,
213
]
} |
metabolic labeling experiments during inositol starvation of cho-k1-ins cells showed that 1 ) the lipid-linked inositol component was maintained at the expense of the soluble inositol pool , 2 ) the decreasing cellular content of phosphatidylinositol was replaced by phosphatidylglycerol , and 3 ) the concentrations of inositol polyphosphates and polyphosphoinositides were conserved at the expense of inositol and phosphatidylinositol . | NA | {
"id": "C0071655",
"name": "polyphosphoinositides",
"pos": [
348,
369
]
} | {
"id": "C0031621",
"name": "phosphatidylinositol",
"pos": [
416,
436
]
} |
these data show that homeostatic mechanisms exist for the maintenance of the polyphosphoinositide and inositol phosphate pools at the expense of inositol and phosphatidylinositol . | NA | {
"id": "C0071655",
"name": "polyphosphoinositide",
"pos": [
77,
97
]
} | {
"id": "C0031621",
"name": "phosphatidylinositol",
"pos": [
158,
178
]
} |
these data show that homeostatic mechanisms exist for the maintenance of the polyphosphoinositide and inositol phosphate pools at the expense of inositol and phosphatidylinositol . | NA | {
"id": "C0021554",
"name": "inositol phosphate",
"pos": [
102,
120
]
} | {
"id": "C0021547",
"name": "inositol",
"pos": [
170,
178
]
} |
to study the effect of triglyceride content of low density lipoprotein ( ldl ) on its physicochemical and biological properties , we have depleted the triglyceride by incubation with hepatic lipase ( hl-ldl ) and raised the triglyceride by incubation of hl-ldl with very low density lipoprotein and lipoprotein-deficient serum . | NA | {
"id": "C0023825",
"name": "very low density lipoprotein",
"pos": [
266,
294
]
} | {
"id": "C0023820",
"name": "lipoprotein",
"pos": [
299,
310
]
} |
the amino acid replacements lie in a glycine-rich region of d-serine dehydratase well removed from pyridoxal 5'-phosphate-binding lysine 118 in the primary structure of the enzyme . | NA | {
"id": "C0002520",
"name": "amino acid",
"pos": [
4,
14
]
} | {
"id": "C0017890",
"name": "glycine",
"pos": [
37,
44
]
} |
however , even though the two proteins were different , tissue-specific mitochondrial isoforms , both formed regularly-sized , perforated cube-like octameric structures with mr of 364,000 +/- 25,000 and 352,000 +/- 20,000 for the cardiac and brain isoform , respectively . | NA | {
"id": "C0033684",
"name": "proteins",
"pos": [
30,
38
]
} | {
"id": "C0597298",
"name": "isoform",
"pos": [
248,
255
]
} |
despite the similarities in the actions of tgf beta 1 and camp on transin gene expression , tgf beta 1 treatment did not significantly elevate intracellular camp levels , thus making it unlikely that camp is a second messenger system for tgf beta 1 action . | NA | {
"id": "C0001413",
"name": "camp",
"pos": [
58,
62
]
} | {
"id": "C0001455",
"name": "camp",
"pos": [
200,
204
]
} |
we report here that in oocytes alpha-sarcin specifically cuts 28 s rrna 377 nucleotides from its 3 ' end , even when used at concentrations that would degrade deproteinized rna . | NA | {
"id": "C0035701",
"name": "rrna",
"pos": [
67,
71
]
} | {
"id": "C0035668",
"name": "rna",
"pos": [
173,
176
]
} |
the proteins of the sarcotoxin i family produced by this cell line were found to have carboxyl-terminal glycine , whereas sarcotoxin i in the hemolymph has amidated amino acids . | NA | {
"id": "C0017890",
"name": "glycine",
"pos": [
104,
111
]
} | {
"id": "C0002520",
"name": "amino acids",
"pos": [
165,
176
]
} |
cloning and sequencing of pro-alpha 1 ( xi ) collagen cdna demonstrates that type xi belongs to the fibrillar class of collagens and reveals that the expression of the gene is not restricted to cartilagenous tissue . | NA | {
"id": "C0009325",
"name": "collagen",
"pos": [
45,
53
]
} | {
"id": "C0969669",
"name": "fibrillar",
"pos": [
100,
109
]
} |
we conclude therefore that pro-alpha 1 ( xi ) collagen belongs to the group of fibrillar collagen genes . | NA | {
"id": "C0009325",
"name": "collagen",
"pos": [
46,
54
]
} | {
"id": "C0969669",
"name": "fibrillar collagen",
"pos": [
79,
97
]
} |
using a competitive enzyme-linked immunosorbent assay , we have found that the guanine nucleotides gtp and guanosine 5'-o- ( thiotriphosphate ) ( gtp gamma s ) ( but not gdp ) and the beta-adrenergic receptor agonist isoproterenol activate gs in native s49 cell membrane by subunit dissociation . | NA | {
"id": "C0018328",
"name": "guanine",
"pos": [
79,
86
]
} | {
"id": "C0018353",
"name": "gtp",
"pos": [
146,
149
]
} |
using a competitive enzyme-linked immunosorbent assay , we have found that the guanine nucleotides gtp and guanosine 5'-o- ( thiotriphosphate ) ( gtp gamma s ) ( but not gdp ) and the beta-adrenergic receptor agonist isoproterenol activate gs in native s49 cell membrane by subunit dissociation . | NA | {
"id": "C0018328",
"name": "guanine",
"pos": [
79,
86
]
} | {
"id": "C0018340",
"name": "gdp",
"pos": [
170,
173
]
} |
using a competitive enzyme-linked immunosorbent assay , we have found that the guanine nucleotides gtp and guanosine 5'-o- ( thiotriphosphate ) ( gtp gamma s ) ( but not gdp ) and the beta-adrenergic receptor agonist isoproterenol activate gs in native s49 cell membrane by subunit dissociation . | NA | {
"id": "C0018353",
"name": "gtp",
"pos": [
99,
102
]
} | {
"id": "C0079469",
"name": "gtp gamma s",
"pos": [
146,
157
]
} |
ca2+ channel agonist ( bay k 8644 ) and antagonist ( nifedipine ) analogs , respectively , enhanced or partially inhibited lh responses to gnrh and also to k+ , the latter confirming the participation of two types of vscc ( dihydropyridine-sensitive and -insensitive ) in k+-induced secretion . | NA | {
"id": "C0028066",
"name": "nifedipine",
"pos": [
53,
63
]
} | {
"id": "C0012315",
"name": "dihydropyridine",
"pos": [
224,
239
]
} |
phorbol esters , including 12-o-tetradecanoylphorbol-13-acetate ( tpa ) , 4 beta-phorbol-12,13-dibenzoate , and 4 beta-phorbol-12,13-diacetate , stimulated lh release with ed50s of 5 , 10 , and 1000 nm , respectively , and with about 70 % of the efficacy of gnrh . | NA | {
"id": "C0031586",
"name": "phorbol esters",
"pos": [
0,
14
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
27,
63
]
} |
as in the parallel reaction with phenylalanine , both the km for the cofactor and the km for the aromatic amino acid increase with this substitution . | NA | {
"id": "C0031453",
"name": "phenylalanine",
"pos": [
33,
46
]
} | {
"id": "C0301713",
"name": "aromatic",
"pos": [
97,
105
]
} |
the metabolism of epitestosterone by the same system is characterized by a marked stereoselectivity in favor of 16 beta-hydroxylation ( 4- to 5-fold relative to 16 alpha-hydroxylation ) , formation of 15 alpha-hydroxyepitestosterone , and a rate of androstenedione formation which is three to five times higher than that observed with testosterone . | NA | {
"id": "C0014594",
"name": "epitestosterone",
"pos": [
18,
33
]
} | {
"id": "C0039601",
"name": "testosterone",
"pos": [
335,
347
]
} |
mass spectral analysis of the androstenedione formed from [ 16,16-2h2 ] testosterone and [ 16,16-2h2 ] epitestosterone indicates essentially complete retention of deuterium , thereby ruling out a mechanism of androstenedione formation via c-16 hydroxylation followed by loss of water and rearrangement . | NA | {
"id": "C0039601",
"name": "testosterone",
"pos": [
72,
84
]
} | {
"id": "C0014594",
"name": "epitestosterone",
"pos": [
103,
118
]
} |
mass spectral analysis of the c-16 hydroxylation products from incubations of testosterone or epitestosterone in 18o2 shows essentially complete incorporation of 18o ( greater than 95 % ) . | NA | {
"id": "C0039601",
"name": "testosterone",
"pos": [
78,
90
]
} | {
"id": "C0014594",
"name": "epitestosterone",
"pos": [
94,
109
]
} |
androstenedione formed from testosterone is enriched in 18o only 2-fold ( 5-8 % ) over background , while the androstenedione formed from epitestosterone shows 84 % enrichment . | NA | {
"id": "C0039601",
"name": "testosterone",
"pos": [
28,
40
]
} | {
"id": "C0014594",
"name": "epitestosterone",
"pos": [
138,
153
]
} |
kinetic experiments utilizing [ 17-2h ] testosterone and [ 17-2h ] epitestosterone as substrates indicate that cleavage of the c-17 carbon-hydrogen bond is involved in a rate-limiting step in the formation of androstenedione from both substrates . | NA | {
"id": "C0039601",
"name": "testosterone",
"pos": [
40,
52
]
} | {
"id": "C0014594",
"name": "epitestosterone",
"pos": [
67,
82
]
} |
thus , beta-carotene metabolism may be an important source of retinoic acid in retinoid target tissues , particularly in species such as humans that are capable of accumulating high concentrations of tissue carotenoids . | NA | {
"id": "C0035339",
"name": "retinoid",
"pos": [
79,
87
]
} | {
"id": "C0007271",
"name": "carotenoids",
"pos": [
207,
218
]
} |
phorbol ester , 12-o-tetradecanoylphorbol-13-acetate ( tpa ) , partially mimics the stimulatory effect of il-6 but reduces that effect of il-1 . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
0,
13
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
16,
52
]
} |
7 ) for several agonists , i.e . active oxygen , phorbol 12-myristate 13-acetate , and serum , tryptic peptide analysis yielded the same phosphopeptides , suggesting that a common s6 kinase is involved in these reactions . | NA | {
"id": "C0030956",
"name": "peptide",
"pos": [
103,
110
]
} | {
"id": "C0031684",
"name": "phosphopeptides",
"pos": [
137,
152
]
} |
the most interesting difference between the structures of rvv-v and other trypsin-type serine proteases is that the conservative ser214-trp215-gly216 sequence ( chymotrypsinogen numbering ) , considered as the site of antiparallel beta-sheet formation between the protein substrate and most serine proteases , has been replaced by the corresponding sequence ala-gly-gly . | NA | {
"id": "C0041236",
"name": "trypsin",
"pos": [
74,
81
]
} | {
"id": "C0036734",
"name": "serine proteases",
"pos": [
291,
307
]
} |
synthesis of gp115 by aorta smooth muscle cells and tendon fibroblasts isolated from chick embryos was investigated . | NA | {
"id": "C0007634",
"name": "cells",
"pos": [
42,
47
]
} | {
"id": "C0016030",
"name": "fibroblasts",
"pos": [
59,
70
]
} |
addition of the in vitro translated protein a to the reconstitution system resulted in low levels of hydroxylase activity suggesting that the subunits of protein a are self-assembling , a supposition supported by the presence of only one protein band on nondenaturing gels . | NA | {
"id": "C0599220",
"name": "subunits",
"pos": [
142,
150
]
} | {
"id": "C0033684",
"name": "protein",
"pos": [
238,
245
]
} |
fluorescence melting experiments indicated that binding of cap analogs stabilized the protein against thermal perturbation and demonstrated subtle differences in folding between the wild-type and mutant forms of the protein . | NA | {
"id": "C0033684",
"name": "protein",
"pos": [
86,
93
]
} | {
"id": "C1564139",
"name": "mutant",
"pos": [
196,
202
]
} |
comparative analysis of the structural organization of the rat cpb , rat cpa1 , and rat cpa2 genes ( gardell , s. j. , craik , c. s. , clauser , e. , goldsmith , e. j. , stewart , c.-b. , graf , m. , and rutter , w. j . ( 1988 ) j. biol . chem . 263 , 17828-17836 ) reveals that , with one exception , the number , position , and sequence composition of the exons in these three carboxypeptidase genes are conserved in spite of considerable divergence with respect to the lengths of their corresponding intervening sequences . | NA | {
"id": "C0054756",
"name": "cpb",
"pos": [
63,
66
]
} | {
"id": "C0007075",
"name": "carboxypeptidase",
"pos": [
379,
395
]
} |
replacement of the prosthetic group of sperm whale myoglobin with zinc protoporphyrin ix prevents h2o2-induced dimerization even when intact horse metmyoglobin is present in the incubation . | NA | {
"id": "C0027078",
"name": "myoglobin",
"pos": [
51,
60
]
} | {
"id": "C0025852",
"name": "metmyoglobin",
"pos": [
147,
159
]
} |
these data suggest that the sensitivity of xp cells towards uv light or ( ac ) 2onfln is due to the same enzymatic defect , namely impaired incision of dna containing pyrimidine dimers or ( ac ) 2onfln-dna adducts . | NA | {
"id": "C0012854",
"name": "dna",
"pos": [
152,
155
]
} | {
"id": "C0242958",
"name": "dna adducts",
"pos": [
202,
213
]
} |
purified golgi membranes of the human intestinal adenocarcinoma cell line caco-2 were used as an antigen to produce a monoclonal antibody , g1/93 , which specifically labels a tubulovesicular compartment near the cis side of the golgi apparatus , including the first cis-cisterna itself , as visualized by single and double immunoelectron microscopy with antibodies against galactosyltransferase . | NA | {
"id": "C0003250",
"name": "monoclonal antibody",
"pos": [
118,
137
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
355,
365
]
} |
using the ts045 mutant of vsv to uncouple the synthesis and transport of the vsv g protein we demonstrated that this distribution was not related to the presence of g protein on the cell surface . | NA | {
"id": "C0007292",
"name": "transport",
"pos": [
60,
69
]
} | {
"id": "C0086376",
"name": "g protein",
"pos": [
165,
174
]
} |
the myosin ii mabs had been shown previously to be cross-reactive with skeletal muscle myosin , with the epitope ( s ) localized to the 50-kd tryptic fragment of the subfragment-1 ( s1 ) domain . | NA | {
"id": "C0027103",
"name": "myosin ii",
"pos": [
4,
13
]
} | {
"id": "C0027096",
"name": "myosin",
"pos": [
87,
93
]
} |
pdgf receptor expression was examined by monoclonal antibodies specific for the b type pdgf receptor and by rna/rna in situ hybridization with a probe constructed from a cdna clone encoding the b type pdgf receptor . | NA | {
"id": "C0290068",
"name": "b",
"pos": [
38,
39
]
} | {
"id": "C0071253",
"name": "pdgf receptor",
"pos": [
201,
214
]
} |
analysis of the glycosaminoglycan composition of the proteoglycan shows an increased amount of chondroitin sulfate , suggesting that the increased labeling per cell may be attributed to an augmented synthesis of chondroitin sulfate glycosaminoglycan on the core protein that also bears heparan sulfate , thus altering the proportions of these two glycosaminoglycans on this hybrid proteoglycan . | NA | {
"id": "C0019143",
"name": "heparan sulfate",
"pos": [
286,
301
]
} | {
"id": "C0017973",
"name": "glycosaminoglycans",
"pos": [
347,
365
]
} |
the results show that plant dna is supercoiled , as is the dna from the other eukaryotes studied , and contains approximately the same concentration of superhelical turns but probably relatively fewer dna superhelical loops . | NA | {
"id": "C0242964",
"name": "plant dna",
"pos": [
22,
31
]
} | {
"id": "C0012854",
"name": "dna",
"pos": [
201,
204
]
} |
antigens are prepared from virus producer cell lines b95-8 , p3hr1 and hh514-c16 ( a superinducible derivative of p3hr1 ) , induced for the expression of early antigens ( ea ) or viral capsid antigens ( vca ) and from the ebv-negative cell lines bjab and ramos . | NA | {
"id": "C0003320",
"name": "antigens",
"pos": [
0,
8
]
} | {
"id": "C0003342",
"name": "viral",
"pos": [
179,
184
]
} |
systematic solid-phase synthesis of all possible overlapping nonapeptides of the 1381 amino acid sequence of the epstein-barr virus major capsid protein ( ebv-mcp ) was used to identify the position of linear antigen epitopes on this protein as recognised by human polyclonal antisera . | NA | {
"id": "C0003320",
"name": "antigen",
"pos": [
209,
216
]
} | {
"id": "C0003316",
"name": "epitopes",
"pos": [
217,
225
]
} |
the viral proteins were visualized on high resolution two dimensional polyacrylamide gels and the proteins recognized by serum antibody from patient gs were detected by hr2d western blot analysis and radioimmunoprecipitation assay . | NA | {
"id": "C0042736",
"name": "viral proteins",
"pos": [
4,
18
]
} | {
"id": "C0033684",
"name": "proteins",
"pos": [
98,
106
]
} |
antibody cross-reactivities towards hblv and other human herpesviruses were assessed by cross-correlation of viral antibody titers against all of the viruses and by cross-absorptions of antisera against the other viruses with hblv . | NA | {
"id": "C0003241",
"name": "antibody",
"pos": [
0,
8
]
} | {
"id": "C0020960",
"name": "antisera",
"pos": [
186,
194
]
} |
hsb-2 cell cultures productively infected with human herpesvirus-6 were treated with the antiviral drugs phosphonoformic acid ( pfa ) , acyclovir ( acv ) , and gancyclovir ( dhpg ) . | NA | {
"id": "C0001367",
"name": "acyclovir",
"pos": [
136,
145
]
} | {
"id": "C0017066",
"name": "dhpg",
"pos": [
174,
178
]
} |
acv and dhpg showed significant toxic effects on uninfected hsb-2 cells , yet only incompletely inhibited viral expression upon infection of the cells . | NA | {
"id": "C0001367",
"name": "acv",
"pos": [
0,
3
]
} | {
"id": "C0017066",
"name": "dhpg",
"pos": [
8,
12
]
} |
in addition , new elisa tests have been developed which measure antibodies in different immunoglobulin classes against the purified polypeptides . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
64,
74
]
} | {
"id": "C0021017",
"name": "immunoglobulin classes",
"pos": [
88,
110
]
} |
human insulin was delivered in a pulsatile manner ( 1.3 mu/kg.min for 2 min , followed by 11 min during which no insulin was infused ) or continuously ( 0.2 mu/kg.min ) for 325 min . | NA | {
"id": "C0795635",
"name": "human insulin",
"pos": [
0,
13
]
} | {
"id": "C0021641",
"name": "insulin",
"pos": [
113,
120
]
} |
thus , we measured the maximal steady state binding in pbmc by incubating the cells with [ 3h ] e2 at 4 c for 150 min . | NA | {
"id": "C1321301",
"name": "pbmc",
"pos": [
55,
59
]
} | {
"id": "C0007634",
"name": "the cells",
"pos": [
74,
83
]
} |
the purpose of this study was to determine if the phorbol ester 12-o-tetradecanoylphorbol-13-acetate ( tpa ) , a potent stimulator of protein kinase-c activity , stimulates steroidogenesis in fz cells and to characterize protein kinase-c activity in fz , neocortex zone , and anencephalic adrenal tissues . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
50,
63
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
64,
100
]
} |
we tested the possibility that the activation of protein kinase-c by the tumor-promoter phorbol ester 12-o-tetradecanoylphorbol-13-acetate ( tpa ) or diacylglycerol stimulates the production of prostaglandin e2 ( pge2 ) by the amnion . | NA | {
"id": "C0031586",
"name": "phorbol ester",
"pos": [
88,
101
]
} | {
"id": "C0039654",
"name": "12-o-tetradecanoylphorbol-13-acetate",
"pos": [
102,
138
]
} |
similar to igg response kinetics , igm levels rose to a peak 6-10 days after detection of parasitemia and showed an apparent serum half-life of less than 25 days . | NA | {
"id": "C0022702",
"name": "kinetics",
"pos": [
24,
32
]
} | {
"id": "C0018517",
"name": "half-life",
"pos": [
131,
140
]
} |
these cells suppressed the production of anti-dna by both autologous peripheral blood mononuclear cells ( pbmc ) and allogeneic pbmc derived from systemic lupus erythematosus ( sle ) patients . | NA | {
"id": "C0007634",
"name": "cells",
"pos": [
6,
11
]
} | {
"id": "C1321301",
"name": "peripheral blood",
"pos": [
69,
85
]
} |
the samples were analysed with regard to pregnanediol glucuronide ( p2-g ) , oestrone glucuronide ( e1-g ) , oestradiol ( e2 ) , progesterone ( p4 ) , lh and creatinine . | NA | {
"id": "C0033004",
"name": "pregnanediol",
"pos": [
41,
53
]
} | {
"id": "C0033308",
"name": "progesterone",
"pos": [
129,
141
]
} |
of antibodies to eight different serotypes present in the serum samples collected 7 to 12 years previously were present in the freshly collected sera , indicating that of antibody persists in human antisera for many years . | NA | {
"id": "C0003241",
"name": "antibody",
"pos": [
171,
179
]
} | {
"id": "C0020960",
"name": "antisera",
"pos": [
198,
206
]
} |
we tested urine specimens from 222 patients with serogroup 1 legionella pneumophila pneumonia in two enzyme-linked immunosorbent assays ( elisas ) which used different monoclonal antibodies ( a and b ) as detector antibodies . | NA | {
"id": "C0003250",
"name": "monoclonal antibodies",
"pos": [
168,
189
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
214,
224
]
} |
we conclude that at least two different epitopes exist on the antigen molecules in urine from patients with serogroup 1 l. pneumophila pneumonia and that the subtyping of urinary antigens can be useful epidemiologically . | NA | {
"id": "C0003316",
"name": "epitopes",
"pos": [
40,
48
]
} | {
"id": "C0003320",
"name": "antigens",
"pos": [
179,
187
]
} |
the use of anti-pc or d3114/63 mabs instead of a pooled serum such as omniserum has several advantages : ( i ) use of a single cross-reactive antibody rather than 83 pooled antibodies ; | NA | {
"id": "C0003250",
"name": "mabs",
"pos": [
31,
35
]
} | {
"id": "C0003241",
"name": "antibodies",
"pos": [
173,
183
]
} |
vibrio-like isolates from atlantic salmon ( salmo salar linnaeas ) and a few from rainbow trout ( s. gairdneri richardson ) suffering from hemorrhagic syndrome ( hitra disease ) , also called cold-water vibriosis , a disease of great importance in norwegian fish farming , were examined for plasmid content . | NA | {
"id": "C0039082",
"name": "syndrome",
"pos": [
151,
159
]
} | {
"id": "C0012634",
"name": "disease",
"pos": [
217,
224
]
} |
the elisa employs a monoclonal antibody specific for a 65-kilodalton surface polypeptide of t. vaginalis as the capture antibody in a sandwich format . | NA | {
"id": "C0003250",
"name": "monoclonal",
"pos": [
20,
30
]
} | {
"id": "C0003241",
"name": "antibody",
"pos": [
120,
128
]
} |
we evaluated the relative sensitivities of two cell systems ( rabbit kidney [ rk ] and human diploid fibroblast [ df ; human embryonic tonsil ] ) in standard tube cultures versus df cells in a 48-well microtiter plate system for the detection of both symptomatic and asymptomatic herpes simplex virus ( hsv ) infection . | NA | {
"id": "C0007634",
"name": "the",
"pos": [
13,
16
]
} | {
"id": "C0016030",
"name": "fibroblast",
"pos": [
101,
111
]
} |
these results suggest that the a antigen can be described by more than one epitope and that a superior serotyping reagent can be prepared from a defined mixture of mabs . | NA | {
"id": "C0003320",
"name": "antigen",
"pos": [
33,
40
]
} | {
"id": "C0003316",
"name": "epitope",
"pos": [
75,
82
]
} |
g alpha g antibodies were mainly distributed between immunoglobulin classes g and m in patients with active ka and in antimonate-treated patients with clinically cured ka . | NA | {
"id": "C0003241",
"name": "antibodies",
"pos": [
10,
20
]
} | {
"id": "C0021017",
"name": "immunoglobulin classes",
"pos": [
53,
75
]
} |
a dna molecular hybridization technique employing a purified adw subtype hepatitis b virus ( hbv ) cloned dna of 3.2 kilobase pairs as a probe was used to screen for the presence of hbv dna in blood samples collected from 486 apparently healthy blood donors . | NA | {
"id": "C0000702",
"name": "a dna",
"pos": [
0,
5
]
} | {
"id": "C0012854",
"name": "dna",
"pos": [
186,
189
]
} |
with cefotaxime , ceftizoxime , and ceftriaxone , a single susceptible-zone size , greater than or equal to 26 mm , is proposed . | NA | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
5,
15
]
} | {
"id": "C0007560",
"name": "ceftizoxime",
"pos": [
18,
29
]
} |
with cefotaxime , ceftizoxime , and ceftriaxone , a single susceptible-zone size , greater than or equal to 26 mm , is proposed . | NA | {
"id": "C0007554",
"name": "cefotaxime",
"pos": [
5,
15
]
} | {
"id": "C0007561",
"name": "ceftriaxone",
"pos": [
36,
47
]
} |
the abbott enzyme immunoassay detected hiv earlier than rta whether or not the patients were antigenemic and appears to be superior to rta for detecting hiv in cultures used for monitoring clinical trials . | NA | {
"id": "C0035379",
"name": "rta",
"pos": [
56,
59
]
} | {
"id": "C1955880",
"name": "rta",
"pos": [
135,
138
]
} |
we studied the expression and distribution of phosphorylated neurofilament ( pnf ) epitopes , nonphosphorylated neurofilament ( npnf ) epitopes , synapse-associated antigens , glial fibrillary acidic protein ( gfap ) and myelin basic protein ( mbp ) . | NA | {
"id": "C0003316",
"name": "epitopes",
"pos": [
83,
91
]
} | {
"id": "C0003320",
"name": "antigens",
"pos": [
165,
173
]
} |
the dna , rna , and protein of apo c-ii have been analyzed in a patient with apo c-ii deficiency ( apo c-iihamburg ) . | NA | {
"id": "C0052190",
"name": "apo c-ii",
"pos": [
31,
39
]
} | {
"id": "C0003594",
"name": "apo c",
"pos": [
99,
104
]
} |
in two cases of gastric cancer , dna from the metastatic tumor had a different dna fingerprint from that found in the primary carcinoma . | NA | {
"id": "C0012854",
"name": "dna",
"pos": [
33,
36
]
} | {
"id": "C0000702",
"name": "a",
"pos": [
134,
135
]
} |
in contrast , neither hemorrhagic hypotension ( 50 mmhg ) nor hypoxia ( 6-8 % o2 ) increased pancreatic ne output ( delta = +80 +/- 110 and -20 +/- 60 pg/min , respectively , p less than 0.01 vs. neuroglucopenia ) despite both producing increases of arterial plasma ne and epinephrine similar to glucopenia . | NA | {
"id": "C0028351",
"name": "ne",
"pos": [
14,
16
]
} | {
"id": "C0014563",
"name": "epinephrine",
"pos": [
273,
284
]
} |
sm-1.2 , a monoclonal antibody that recognizes igf-i and igf-ii but not insulin , inhibited basal dna synthesis in frtl5 and m12 cells and the mitogenic effects in frtl5 of agents that are synergized by igf , such as tsh , forskolin , bt2camp , and graves'-igg . | NA | {
"id": "C0021665",
"name": "igf-i",
"pos": [
47,
52
]
} | {
"id": "C0037657",
"name": "igf",
"pos": [
203,
206
]
} |
sm-1.2 , a monoclonal antibody that recognizes igf-i and igf-ii but not insulin , inhibited basal dna synthesis in frtl5 and m12 cells and the mitogenic effects in frtl5 of agents that are synergized by igf , such as tsh , forskolin , bt2camp , and graves'-igg . | NA | {
"id": "C0021666",
"name": "igf-ii",
"pos": [
57,
63
]
} | {
"id": "C0037657",
"name": "igf",
"pos": [
203,
206
]
} |
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